Rmu_sc0001118.1_g000022

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001118.1
OG0011757
Physical Location & Seq
Forward (+)
136587 .. 139067
2481 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2187 bp
atgcatttcgagacacaagccaaaccggttgttgagtgttggcagagttcagatgagattgtggaattactttcatcccatgagattaaagaggagagcaaagtttcagttcttgactcaaaggatttggatttcgatatgcattttgagacgcaagccaaaccggttgttgagtgttgtcccagttcagatgagattgtggaattactttcatcccatgagattaaagaggagagcaaagtttcagttcttgactcaaaggatttggggcagaatggagaagaagttgaagaaaatgagcaagctgaagatactactagtactcgagcaacaccagcctcttgtcatgaggatgacagtcgatcaaatgctgctgacatagtaggaagagacattgattcagatgttcatcaagatgagattgtggtactttcatcccatgagattaaagaggagaacaaagtttcagttcttgactcaaaggatttggggcagaatggagaagaagttgaagaaaatgagcaagctgaagatactactagtactcgagcaacaccatcctcttgtcatgaggatgacagtcaatcaaatgctgctgacatagtaggaagagacattgattcagatgttcatcaagcaacaactgaggatgatgacatccatattcggaacgatgagattgatacagaggtctcaatagggactgatattcctgatcatgatatattcaatgaggatattcaatcagctcatgaacttctacattcttatcccggtgtgcaagaagatccttctacaagttctcccaatgctgaaattgatgataatcatggtgctaagcaagaagaagagtttctagaattcaagaccttatcagttgaaacaagtgagaaagaacaaaatagttatttctcattaggttcagaacttaatgagattgaggaagagaaggttcttcctgatacaccaacttctatagacaatttccatcagttgtataatagaatgctactaactgagagaagggaatcaggaacagaagcatcattggatggaagtgtagtgagcgacattgaatgtggtgatggagtcctgactatggagaagctgaaatcggtgttgagagctgaacggaaagctttaaatagagtatattcagaacttgaagaagagaggagtgcttctgcaatagcagccaaccagacaatggcaatgataaataggctacaagaagaaaaggcagcaatgcagatggaagctttgcagtaccaaaggatgatggatgagcagtctgagtacgaccaggaagctatgcagctcttaaacgagctcatggtaaagagggaaaaggaaaagcaagaacttgaaaaggagctagaaatatgtcgaaagaaggtccaagattatgaggccaaagagaaaatgatgattttgaggagaatgaaagagggctgcacaagaagtaggacttcatctggtccttgtagcaatgctgaggatagtgatggactttctattgatttgaatcatgaagtgaaggaagaagataacctctcgggacatgaagagagtagcaatcaaaacacaccaagggatgcggtgttatatttagaagaatctttggctagctttgaggaagagaagttatccattctagaccaactcaaggaattggaagagaagcttttgaccttgaatgacgaagaggaagaagaacattacgaggatataaaaccaattgatcatttattttcagagaatgggaatggctaccatgagaatggtgatgttggcggtgaagaaaatggcattgcaaatggccactccaaagaaatgaatggaacacatcatcaagatagaaaaattatgggatcaattaaggcaaagagacttcttccacttttcaatgaagctgaagatgaagacgaggagttgaatggagatgtagaaggttatgattctgttgcatcacaagattacaagaaactcgctattgaagaggaggttgcgcatgtctatgagaggctacaagcacttgaagcggatagagagtttctaaagcactgtattagctccttaaggaaaggtgacaagggattagatcttctccaagaaattttgcaacatcttcgtgacctgagaagtgtggaaacccgtctaaggaattcaggagagggtatcctataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

728

Amino Acids

82.34

Weight (kDa)

4.38

Isoelectric Point (pI)

58.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 369 - 459 5.5e-32 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011757)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G70750
fragaria_vesca FvH4_4g34051
malus_domestica MD13G1021300.v1.1 MD16G1022600.v1.1
prunus_persica Prupe.1G330400_v2.0.a1
pyrus_communis pycom16g01990
rosa_chinensis RchiOBHm_Chr4g0443551
rosa_laevigata RLG00000005917
rosa_multiflora Rmu_co8317059.1_g000001 Rmu_sc0001118.1_g000021 Rmu_sc0001118.1_g000022
rosa_roxburghii Rroxscaffold_5G00384100
rosa_samantha Rh4AG402900 Rh4AG404600 Rh4BG415600 Rh4CG430200 Rh4DG410100 Rh4DG411300
rosa_wichuraiana Rw4G034740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2010
AccB7I CCANNNNNTGG 1 cut(s) 1207
AciI CCGC 3 cut(s) 1598, 1794, 2042
AclWI GGATC 2 cut(s) 782, 1879
AcoI YGGCCR 1 cut(s) 1819
AcsI RAATTY 3 cut(s) 860, 2115, 2164
AcuI CTGAAG 3 cut(s) 327, 549, 1935
AfaI GTAC 5 cut(s) 322, 429, 544, 1268, 1298
AfiI CCNNNNNNNGG 4 cut(s) 1099, 1207, 1666, 2160
AflII CTTAAG 1 cut(s) 2077
AgeI ACCGGT 2 cut(s) 25, 163
AhlI ACTAGT 2 cut(s) 317, 539
AjnI CCWGG 1 cut(s) 1302
Alw21I GWGCWC 1 cut(s) 1332
Alw26I GTCTC 6 cut(s) 5, 143, 384, 606, 697, 1882
AlwI GGATC 2 cut(s) 782, 1879
Ama87I CYCGRG 3 cut(s) 324, 546, 1555
AoxI GGCC 2 cut(s) 1410, 1819
ApeKI GCWGC 6 cut(s) 371, 593, 1193, 1241, 1315, 1452
ApoI RAATTY 3 cut(s) 860, 2115, 2164
AsiGI ACCGGT 2 cut(s) 25, 163
Asp700I GAANNNNTTC 1 cut(s) 852
AspLEI GCGC 1 cut(s) 2011
AspS9I GGNCC 2 cut(s) 1396, 1478
AsuC2I CCSGG 1 cut(s) 774
AsuHPI GGTGA 4 cut(s) 1094, 1796, 1808, 2099
AsuNHI GCTAGC 1 cut(s) 1625
AvaI CYCGRG 3 cut(s) 324, 546, 1555
AvaII GGWCC 2 cut(s) 1396, 1478
BaeI ACNNNNGTAYC 2 cut(s) 675, 708
BalI TGGCCA 1 cut(s) 1821
BanII GRGCYC 1 cut(s) 1332
BbsI GAAGAC 1 cut(s) 1929
Bbv12I GWGCWC 1 cut(s) 1332
BbvCI CCTCAGC 1 cut(s) 1494
BbvI GCAGC 6 cut(s) 358, 580, 1205, 1253, 1327, 1439
BccI CCATC 7 cut(s) 565, 996, 1046, 1079, 1246, 1273, 1499
BcgI CGANNNNNNTGC 2 cut(s) 2111, 2145
BciT130I CCWGG 1 cut(s) 1304
BclI TGATCA 2 cut(s) 715, 1741
BcnI CCSGG 1 cut(s) 774
BcoDI GTCTC 6 cut(s) 5, 143, 384, 606, 697, 1882
BcuI ACTAGT 2 cut(s) 317, 539
BfaI CTAG 6 cut(s) 318, 540, 857, 1376, 1626, 1655
BfmI CTRYAG 1 cut(s) 975
BfrI CTTAAG 1 cut(s) 2077
BglII AGATCT 1 cut(s) 2101
BisI GCNGC 6 cut(s) 372, 594, 1194, 1242, 1316, 1453
BlpI GCTNAGC 1 cut(s) 837
BlsI GCNGC 6 cut(s) 373, 595, 1195, 1243, 1317, 1454
BmcAI AGTACT 2 cut(s) 322, 544
Bme1390I CCNGG 2 cut(s) 774, 1304
Bme18I GGWCC 2 cut(s) 1396, 1478
BmeT110I CYCGRG 3 cut(s) 324, 546, 1555
BmgT120I GGNCC 2 cut(s) 1396, 1478
BmrFI CCNGG 2 cut(s) 774, 1304
BmrI ACTGGG 1 cut(s) 177
BmsI GCATC 3 cut(s) 1052, 1585, 1976
BmtI GCTAGC 1 cut(s) 1629
BmuI ACTGGG 1 cut(s) 177
BpiI GAAGAC 1 cut(s) 1929
Bpu10I CCTNAGC 1 cut(s) 1494
Bpu1102I GCTNAGC 1 cut(s) 837
BpuEI CTTGAG 1 cut(s) 1649
BpuMI CCSGG 1 cut(s) 774
BsaBI GATNNNNATC 4 cut(s) 408, 630, 825, 1524
BsaI GGTCTC 1 cut(s) 697
BsaJI CCNNGG 1 cut(s) 1589
BsaWI WCCGGW 2 cut(s) 25, 163
BsaXI ACNNNNNCTCC 6 cut(s) 270, 300, 492, 522, 787, 817
Bsc4I CCNNNNNNNGG 4 cut(s) 1099, 1207, 1666, 2160
Bse118I RCCGGY 2 cut(s) 25, 163
Bse1I ACTGG 1 cut(s) 183
Bse3DI GCAATG 4 cut(s) 1218, 1251, 1495, 1809
Bse8I GATNNNNATC 4 cut(s) 408, 630, 825, 1524
BseBI CCWGG 1 cut(s) 1304
BseDI CCNNGG 1 cut(s) 1589
BseJI GATNNNNATC 4 cut(s) 408, 630, 825, 1524
BseLI CCNNNNNNNGG 4 cut(s) 1099, 1207, 1666, 2160
BseMI GCAATG 4 cut(s) 1218, 1251, 1495, 1809
BseMII CTCAG 5 cut(s) 636, 1008, 1284, 1485, 2129
BseNI ACTGG 1 cut(s) 183
BseRI GAGGAG 7 cut(s) 107, 245, 467, 1189, 1450, 1943, 2015
BseXI GCAGC 6 cut(s) 358, 580, 1205, 1253, 1327, 1439
BsgI GTGCAG 1 cut(s) 1438
BshFI GGCC 2 cut(s) 1412, 1821
BshTI ACCGGT 2 cut(s) 25, 163
BsiHKAI GWGCWC 1 cut(s) 1332
BsiHKCI CYCGRG 3 cut(s) 324, 546, 1555
BsiSI CCGG 3 cut(s) 26, 164, 774
BslFI GGGAC 3 cut(s) 165, 715, 1572
BslI CCNNNNNNNGG 4 cut(s) 1099, 1207, 1666, 2160
BsmAI GTCTC 6 cut(s) 5, 143, 384, 606, 697, 1882
BsmBI CGTCTC 1 cut(s) 143
BsmFI GGGAC 3 cut(s) 165, 715, 1572
BsmI GAATGC 1 cut(s) 1011
BsnI GGCC 2 cut(s) 1412, 1821
Bso31I GGTCTC 1 cut(s) 697
BsoBI CYCGRG 3 cut(s) 324, 546, 1555
Bsp1286I GDGCHC 1 cut(s) 1332
Bsp143I GATC 6 cut(s) 362, 715, 787, 1741, 1871, 2101
Bsp1720I GCTNAGC 1 cut(s) 837
BspACI CCGC 3 cut(s) 1598, 1794, 2042
BspANI GGCC 2 cut(s) 1412, 1821
BspCNI CTCAG 5 cut(s) 637, 1009, 1285, 1486, 2130
BspHI TCATGA 5 cut(s) 346, 568, 718, 751, 1528
BspOI GCTAGC 1 cut(s) 1629
BspPI GGATC 2 cut(s) 782, 1879
BspTI CTTAAG 1 cut(s) 2077
BspTNI GGTCTC 1 cut(s) 697
BsrDI GCAATG 4 cut(s) 1218, 1251, 1495, 1809
BsrFI RCCGGY 2 cut(s) 25, 163
BsrI ACTGG 1 cut(s) 183
BssAI RCCGGY 2 cut(s) 25, 163
BssECI CCNNGG 1 cut(s) 1589
BssMI GATC 6 cut(s) 362, 715, 787, 1741, 1871, 2101
BssT1I CCWWGG 1 cut(s) 1589
Bst2UI CCWGG 1 cut(s) 1304
Bst4CI ACNGT 3 cut(s) 359, 581, 2066
BstAFI CTTAAG 1 cut(s) 2077
BstC8I GCNNGC 4 cut(s) 156, 303, 525, 1627
BstDEI CTNAG 7 cut(s) 645, 837, 1017, 1293, 1494, 2138, 2159
BstHHI GCGC 1 cut(s) 2011
BstKTI GATC 6 cut(s) 365, 718, 790, 1744, 1874, 2104
BstMAI GTCTC 6 cut(s) 5, 143, 384, 606, 697, 1882
BstMBI GATC 6 cut(s) 362, 715, 787, 1741, 1871, 2101
BstMWI GCNNNNNNNGC 3 cut(s) 335, 1193, 2039
BstNI CCWGG 1 cut(s) 1304
BstNSI RCATGY 1 cut(s) 2015
BstSCI CCNGG 2 cut(s) 772, 1302
BstSFI CTRYAG 1 cut(s) 975
BstV1I GCAGC 6 cut(s) 358, 580, 1205, 1253, 1327, 1439
BstV2I GAAGAC 1 cut(s) 1929
BstX2I RGATCY 2 cut(s) 787, 2101
BstXI CCANNNNNNTGG 1 cut(s) 1781
BstYI RGATCY 2 cut(s) 787, 2101
BsuRI GGCC 2 cut(s) 1412, 1821
BtsIMutI CAGTG 1 cut(s) 2062
Cac8I GCNNGC 4 cut(s) 156, 303, 525, 1627
CciI TCATGA 5 cut(s) 346, 568, 718, 751, 1528
CfoI GCGC 1 cut(s) 2011
Cfr10I RCCGGY 2 cut(s) 25, 163
Cfr13I GGNCC 2 cut(s) 1396, 1478
CseI GACGC 1 cut(s) 160
Csp6I GTAC 5 cut(s) 321, 428, 543, 1267, 1297
CspAI ACCGGT 2 cut(s) 25, 163
CviQI GTAC 5 cut(s) 321, 428, 543, 1267, 1297
DdeI CTNAG 7 cut(s) 645, 837, 1017, 1293, 1494, 2138, 2159
DpnI GATC 6 cut(s) 364, 717, 789, 1743, 1873, 2103
DpnII GATC 6 cut(s) 362, 715, 787, 1741, 1871, 2101
DraI TTTAAA 1 cut(s) 1143
EaeI YGGCCR 1 cut(s) 1819
Ecl136II GAGCTC 1 cut(s) 1330
Eco130I CCWWGG 1 cut(s) 1589
Eco24I GRGCYC 1 cut(s) 1332
Eco31I GGTCTC 1 cut(s) 697
Eco47I GGWCC 2 cut(s) 1396, 1478
Eco53kI GAGCTC 1 cut(s) 1330
Eco57I CTGAAG 3 cut(s) 327, 549, 1935
Eco88I CYCGRG 3 cut(s) 324, 546, 1555
EcoICRI GAGCTC 1 cut(s) 1330
EcoRI GAATTC 2 cut(s) 860, 2164
EcoRII CCWGG 1 cut(s) 1302
EcoT14I CCWWGG 1 cut(s) 1589
EcoT22I ATGCAT 2 cut(s) 6, 144
EcoT38I GRGCYC 1 cut(s) 1332
ErhI CCWWGG 1 cut(s) 1589
Esp3I CGTCTC 1 cut(s) 143
FalI AAGNNNNNCTT 4 cut(s) 1453, 1485, 1668, 1700
FaqI GGGAC 3 cut(s) 165, 715, 1572
FbaI TGATCA 2 cut(s) 715, 1741
Fnu4HI GCNGC 6 cut(s) 372, 594, 1194, 1242, 1316, 1453
FriOI GRGCYC 1 cut(s) 1332
Fsp4HI GCNGC 6 cut(s) 372, 594, 1194, 1242, 1316, 1453
FspBI CTAG 6 cut(s) 318, 540, 857, 1376, 1626, 1655
FspI TGCGCA 1 cut(s) 2010
GlaI GCGC 1 cut(s) 2010
GluI GCNGC 6 cut(s) 372, 594, 1194, 1242, 1316, 1453
HaeIII GGCC 2 cut(s) 1412, 1821
HapII CCGG 3 cut(s) 26, 164, 774
HgaI GACGC 1 cut(s) 160
HhaI GCGC 1 cut(s) 2011
Hin6I GCGC 1 cut(s) 2009
HinP1I GCGC 1 cut(s) 2009
HindIII AAGCTT 3 cut(s) 1137, 1257, 1682
HpaII CCGG 3 cut(s) 26, 164, 774
HphI GGTGA 4 cut(s) 1094, 1796, 1808, 2099
Hpy188I TCNGA 9 cut(s) 52, 190, 403, 625, 669, 925, 1159, 1294, 1756
HpyAV CCTTC 6 cut(s) 801, 943, 1017, 1387, 1531, 1943
HpyCH4III ACNGT 3 cut(s) 359, 581, 2066
HpyF10VI GCNNNNNNNGC 3 cut(s) 335, 1193, 2039
HpyF3I CTNAG 7 cut(s) 645, 837, 1017, 1293, 1494, 2138, 2159
HspAI GCGC 1 cut(s) 2009
Ksp22I TGATCA 2 cut(s) 715, 1741
Kzo9I GATC 6 cut(s) 362, 715, 787, 1741, 1871, 2101
LmnI GCTCC 2 cut(s) 1372, 2078
Lsp1109I GCAGC 6 cut(s) 358, 580, 1205, 1253, 1327, 1439
LweI GCATC 3 cut(s) 1052, 1585, 1976
MaeI CTAG 6 cut(s) 318, 540, 857, 1376, 1626, 1655
MaeIII GTNAC 2 cut(s) 2087, 2132
MalI GATC 6 cut(s) 364, 717, 789, 1743, 1873, 2103
MboI GATC 6 cut(s) 362, 715, 787, 1741, 1871, 2101
MfeI CAATTG 1 cut(s) 1737
MflI RGATCY 2 cut(s) 787, 2101
MhlI GDGCHC 1 cut(s) 1332
MlsI TGGCCA 1 cut(s) 1821
MluNI TGGCCA 1 cut(s) 1821
MlyI GAGTC 4 cut(s) 110, 248, 470, 1098
Mox20I TGGCCA 1 cut(s) 1821
Mph1103I ATGCAT 2 cut(s) 6, 144
MroXI GAANNNNTTC 1 cut(s) 852
MscI TGGCCA 1 cut(s) 1821
MseI TTAA 8 cut(s) 87, 225, 447, 930, 1142, 1322, 1878, 2078
MslI CAYNNNNRTG 6 cut(s) 351, 414, 573, 1779, 2016, 2130
Msp20I TGGCCA 1 cut(s) 1821
MspCI CTTAAG 1 cut(s) 2077
MspI CCGG 3 cut(s) 26, 164, 774
MspR9I CCNGG 2 cut(s) 774, 1304
MunI CAATTG 1 cut(s) 1737
Mva1269I GAATGC 1 cut(s) 1011
MvaI CCWGG 1 cut(s) 1304
MwoI GCNNNNNNNGC 3 cut(s) 335, 1193, 2039
NciI CCSGG 1 cut(s) 774
NdeII GATC 6 cut(s) 362, 715, 787, 1741, 1871, 2101
NheI GCTAGC 1 cut(s) 1625
NmuCI GTSAC 2 cut(s) 2087, 2132
NsbI TGCGCA 1 cut(s) 2010
NsiI ATGCAT 2 cut(s) 6, 144
NspI RCATGY 1 cut(s) 2015
PaeR7I CTCGAG 2 cut(s) 324, 546
PagI TCATGA 5 cut(s) 346, 568, 718, 751, 1528
PctI GAATGC 1 cut(s) 1011
PdmI GAANNNNTTC 1 cut(s) 852
PfeI GAWTC 6 cut(s) 398, 620, 1028, 1525, 1616, 1958
PflMI CCANNNNNTGG 1 cut(s) 1207
PinAI ACCGGT 2 cut(s) 25, 163
PkrI GCNGC 6 cut(s) 373, 595, 1195, 1243, 1317, 1454
PleI GAGTC 4 cut(s) 110, 248, 470, 1097
PpsI GAGTC 4 cut(s) 110, 248, 470, 1097
Psp124BI GAGCTC 1 cut(s) 1332
Psp6I CCWGG 1 cut(s) 1302
PspGI CCWGG 1 cut(s) 1302
PspPI GGNCC 2 cut(s) 1396, 1478
PspXI VCTCGAGB 2 cut(s) 324, 546
PsuI RGATCY 2 cut(s) 787, 2101
RsaI GTAC 5 cut(s) 322, 429, 544, 1268, 1298
RsaNI GTAC 5 cut(s) 321, 428, 543, 1267, 1297
RseI CAYNNNNRTG 6 cut(s) 351, 414, 573, 1779, 2016, 2130
SacI GAGCTC 1 cut(s) 1332
SaqAI TTAA 8 cut(s) 87, 225, 447, 930, 1142, 1322, 1878, 2078
SatI GCNGC 6 cut(s) 372, 594, 1194, 1242, 1316, 1453
Sau3AI GATC 6 cut(s) 362, 715, 787, 1741, 1871, 2101
Sau96I GGNCC 2 cut(s) 1396, 1478
ScaI AGTACT 2 cut(s) 322, 544
SchI GAGTC 4 cut(s) 110, 248, 470, 1098
ScrFI CCNGG 2 cut(s) 774, 1304
SduI GDGCHC 1 cut(s) 1332
SfaNI GCATC 3 cut(s) 1052, 1585, 1976
SfcI CTRYAG 1 cut(s) 975
Sfr274I CTCGAG 2 cut(s) 324, 546
SinI GGWCC 2 cut(s) 1396, 1478
SlaI CTCGAG 2 cut(s) 324, 546
SmiMI CAYNNNNRTG 6 cut(s) 351, 414, 573, 1779, 2016, 2130
SmlI CTYRAG 4 cut(s) 324, 546, 1664, 2077
SmoI CTYRAG 4 cut(s) 324, 546, 1664, 2077
SpeI ACTAGT 2 cut(s) 317, 539
SsiI CCGC 3 cut(s) 1598, 1794, 2042
SspMI CTAG 6 cut(s) 318, 540, 857, 1376, 1626, 1655
SstI GAGCTC 1 cut(s) 1332
StyD4I CCNGG 2 cut(s) 772, 1302
StyI CCWWGG 1 cut(s) 1589
TaaI ACNGT 3 cut(s) 359, 581, 2066
TaqI TCGA 6 cut(s) 9, 135, 325, 361, 547, 1387
TatI WGTACW 2 cut(s) 320, 542
TfiI GAWTC 6 cut(s) 398, 620, 1028, 1525, 1616, 1958
Tru1I TTAA 8 cut(s) 87, 225, 447, 930, 1142, 1322, 1878, 2078
Tru9I TTAA 8 cut(s) 87, 225, 447, 930, 1142, 1322, 1878, 2078
TscAI CASTG 1 cut(s) 2069
TseFI GTSAC 2 cut(s) 2087, 2132
TseI GCWGC 6 cut(s) 371, 593, 1193, 1241, 1315, 1452
Tsp45I GTSAC 2 cut(s) 2087, 2132
TspGWI ACGGA 1 cut(s) 1147
TspRI CASTG 1 cut(s) 2069
Van91I CCANNNNNTGG 1 cut(s) 1207
Vha464I CTTAAG 1 cut(s) 2077
VpaK11BI GGWCC 2 cut(s) 1396, 1478
XapI RAATTY 3 cut(s) 860, 2115, 2164
XbaI TCTAGA 2 cut(s) 856, 1654
XceI RCATGY 1 cut(s) 2015
XcmI CCANNNNNNNNNTGG 1 cut(s) 1204
XhoI CTCGAG 2 cut(s) 324, 546
XmnI GAANNNNTTC 1 cut(s) 852
XspI CTAG 6 cut(s) 318, 540, 857, 1376, 1626, 1655
ZrmI AGTACT 2 cut(s) 322, 544
Zsp2I ATGCAT 2 cut(s) 6, 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.