Rmu_sc0001181.1_g000008

2,3-bisphosphoglycerate-independent phosphoglycerate mutase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001181.1
OG0005100
Physical Location & Seq
Reverse (-)
41486 .. 42456
971 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 711 bp
atggtagctccaacaaaaatcattgctggattgggcttatctcttggcattgatatcctggaagctcctggagcaactggagactacagaacacttctaactttcaaagcaactgcaatagccaaggcactctcggctcctctgcagtcttgccccaatgtgtttgtgccaggggaagatgaacacaaaccaggccgatcagatggctatgattttgggttcctccacataaaggctatagatgatgcaggtcatgacaaggcaaccattttcaaagtcaaaggattggaagctgtggatcgagctatagggcagctggccaggctcctttgggagactgaatcaactggaaaatttcagtactttctttgtgttacaggagaccactctaccccagttgaatatggagaccacagctttgagtcagttccgttcacaatatgttcattgaaagattttgtgggtgttgtaggtgtggaaacgattttgggttgttcgcttgatccatttcctcttccaaccgtaaaagagtgtgaatatccaacagaacatgtcgagatggaacaagagcccaccaaacagcctcgagctttcagtggtgattcagtttgtgagtttaatgagatagcggcagcaagaggatgtcttggacgtttttctggcggtgagatgatgggagtaataaagaatttcctggagctagaagcatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metalloenzyme PhosphMutase

Protein Analysis

236

Amino Acids

25.47

Weight (kDa)

4.8

Isoelectric Point (pI)

32.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metalloenzyme PF01676 68 - 149 2.4e-14 Metalloenzyme superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 239
AciI CCGC 2 cut(s) 629, 663
AclWI GGATC 2 cut(s) 306, 497
AcoI YGGCCR 1 cut(s) 318
AcsI RAATTY 2 cut(s) 353, 688
AfaI GTAC 1 cut(s) 362
AfiI CCNNNNNNNGG 1 cut(s) 232
AflIII ACRYGT 1 cut(s) 550
AgsI TTSAA 4 cut(s) 106, 274, 401, 451
AjnI CCWGG 6 cut(s) 57, 67, 169, 190, 320, 693
AluBI AGCT 8 cut(s) 8, 65, 293, 305, 316, 417, 590, 700
AluI AGCT 8 cut(s) 8, 65, 293, 305, 316, 417, 590, 700
Alw26I GTCTC 4 cut(s) 75, 329, 375, 402
AlwI GGATC 2 cut(s) 306, 497
Ama87I CYCGRG 1 cut(s) 585
AoxI GGCC 2 cut(s) 193, 318
ApeKI GCWGC 2 cut(s) 313, 632
ApoI RAATTY 2 cut(s) 353, 688
ArsI GACNNNNNNTTYG 2 cut(s) 401, 433
AsuHPI GGTGA 2 cut(s) 611, 677
AvaI CYCGRG 1 cut(s) 585
BalI TGGCCA 1 cut(s) 320
BanII GRGCYC 1 cut(s) 573
BbvI GCAGC 2 cut(s) 325, 644
BccI CCATC 3 cut(s) 197, 553, 667
BciT130I CCWGG 6 cut(s) 59, 69, 171, 192, 322, 695
BcoDI GTCTC 4 cut(s) 75, 329, 375, 402
BfaI CTAG 1 cut(s) 701
BfmI CTRYAG 4 cut(s) 85, 143, 237, 306
BfuAI ACCTGC 1 cut(s) 239
BisI GCNGC 3 cut(s) 314, 630, 633
BlsI GCNGC 3 cut(s) 315, 631, 634
BmcAI AGTACT 1 cut(s) 362
Bme1390I CCNGG 6 cut(s) 59, 69, 171, 192, 322, 695
BmeT110I CYCGRG 1 cut(s) 585
BmiI GGNNCC 3 cut(s) 138, 221, 326
BmrFI CCNGG 6 cut(s) 59, 69, 171, 192, 322, 695
BmrI ACTGGG 1 cut(s) 389
BmsI GCATC 1 cut(s) 235
BmuI ACTGGG 1 cut(s) 389
BpmI CTGGAG 2 cut(s) 90, 99
BsaI GGTCTC 2 cut(s) 375, 402
BsaJI CCNNGG 2 cut(s) 123, 170
Bsc4I CCNNNNNNNGG 1 cut(s) 232
Bse1I ACTGG 3 cut(s) 82, 352, 395
Bse3DI GCAATG 1 cut(s) 21
BseBI CCWGG 6 cut(s) 59, 69, 171, 192, 322, 695
BseDI CCNNGG 2 cut(s) 123, 170
BseGI GGATG 1 cut(s) 647
BseLI CCNNNNNNNGG 1 cut(s) 232
BseMI GCAATG 1 cut(s) 21
BseNI ACTGG 3 cut(s) 82, 352, 395
BseRI GAGGAG 1 cut(s) 129
BseXI GCAGC 2 cut(s) 325, 644
BshFI GGCC 2 cut(s) 195, 320
BsiHKCI CYCGRG 1 cut(s) 585
BslI CCNNNNNNNGG 1 cut(s) 232
BsmAI GTCTC 4 cut(s) 75, 329, 375, 402
BsnI GGCC 2 cut(s) 195, 320
Bso31I GGTCTC 2 cut(s) 375, 402
BsoBI CYCGRG 1 cut(s) 585
Bsp1286I GDGCHC 1 cut(s) 573
Bsp143I GATC 3 cut(s) 197, 298, 502
BspACI CCGC 2 cut(s) 629, 663
BspANI GGCC 2 cut(s) 195, 320
BspHI TCATGA 1 cut(s) 253
BspLI GGNNCC 3 cut(s) 138, 221, 326
BspMAI CTGCAG 1 cut(s) 147
BspMI ACCTGC 1 cut(s) 239
BspPI GGATC 2 cut(s) 306, 497
BspTNI GGTCTC 2 cut(s) 375, 402
BsrDI GCAATG 1 cut(s) 21
BsrI ACTGG 3 cut(s) 82, 352, 395
BssECI CCNNGG 2 cut(s) 123, 170
BssMI GATC 3 cut(s) 197, 298, 502
BssT1I CCWWGG 1 cut(s) 123
Bst2UI CCWGG 6 cut(s) 59, 69, 171, 192, 322, 695
Bst4CI ACNGT 1 cut(s) 523
Bst6I CTCTTC 1 cut(s) 519
BstC8I GCNNGC 1 cut(s) 318
BstF5I GGATG 1 cut(s) 647
BstKTI GATC 3 cut(s) 200, 301, 505
BstMAI GTCTC 4 cut(s) 75, 329, 375, 402
BstMBI GATC 3 cut(s) 197, 298, 502
BstMWI GCNNNNNNNGC 3 cut(s) 71, 134, 322
BstNI CCWGG 6 cut(s) 59, 69, 171, 192, 322, 695
BstNSI RCATGY 1 cut(s) 554
BstSCI CCNGG 6 cut(s) 57, 67, 169, 190, 320, 693
BstSFI CTRYAG 4 cut(s) 85, 143, 237, 306
BstV1I GCAGC 2 cut(s) 325, 644
BsuRI GGCC 2 cut(s) 195, 320
BtsCI GGATG 1 cut(s) 647
BtsIMutI CAGTG 1 cut(s) 601
BveI ACCTGC 1 cut(s) 239
Cac8I GCNNGC 1 cut(s) 318
CciI TCATGA 1 cut(s) 253
Csp6I GTAC 1 cut(s) 361
CviAII CATG 3 cut(s) 254, 551, 708
CviQI GTAC 1 cut(s) 361
DpnI GATC 3 cut(s) 199, 300, 504
DpnII GATC 3 cut(s) 197, 298, 502
EaeI YGGCCR 1 cut(s) 318
Eam1104I CTCTTC 1 cut(s) 519
EarI CTCTTC 1 cut(s) 519
Eco130I CCWWGG 1 cut(s) 123
Eco24I GRGCYC 1 cut(s) 573
Eco31I GGTCTC 2 cut(s) 375, 402
Eco32I GATATC 1 cut(s) 55
Eco88I CYCGRG 1 cut(s) 585
EcoRII CCWGG 6 cut(s) 57, 67, 169, 190, 320, 693
EcoRV GATATC 1 cut(s) 55
EcoT14I CCWWGG 1 cut(s) 123
EcoT38I GRGCYC 1 cut(s) 573
ErhI CCWWGG 1 cut(s) 123
FaeI CATG 3 cut(s) 257, 554, 711
FaiI YATR 9 cut(s) 210, 230, 239, 255, 308, 405, 442, 552, 709
FatI CATG 3 cut(s) 253, 550, 707
Fnu4HI GCNGC 3 cut(s) 314, 630, 633
FokI GGATG 1 cut(s) 654
FriOI GRGCYC 1 cut(s) 573
Fsp4HI GCNGC 3 cut(s) 314, 630, 633
FspBI CTAG 1 cut(s) 701
GluI GCNGC 3 cut(s) 314, 630, 633
GsuI CTGGAG 2 cut(s) 90, 99
HaeIII GGCC 2 cut(s) 195, 320
Hin1II CATG 3 cut(s) 257, 554, 711
HinfI GANTC 3 cut(s) 341, 422, 602
HphI GGTGA 2 cut(s) 611, 677
Hpy166II GTNNAC 1 cut(s) 435
Hpy188I TCNGA 1 cut(s) 202
Hpy188III TCNNGA 2 cut(s) 254, 556
Hpy8I GTNNAC 1 cut(s) 435
HpyCH4III ACNGT 1 cut(s) 523
HpyCH4IV ACGT 1 cut(s) 652
HpyCH4V TGCA 3 cut(s) 116, 145, 248
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 134, 322
HpySE526I ACGT 1 cut(s) 652
Hsp92II CATG 3 cut(s) 257, 554, 711
Kzo9I GATC 3 cut(s) 197, 298, 502
LmnI GCTCC 6 cut(s) 13, 70, 71, 142, 330, 697
Lsp1109I GCAGC 2 cut(s) 325, 644
LweI GCATC 1 cut(s) 235
MaeI CTAG 1 cut(s) 701
MaeII ACGT 1 cut(s) 652
MaeIII GTNAC 1 cut(s) 373
MalI GATC 3 cut(s) 199, 300, 504
MboI GATC 3 cut(s) 197, 298, 502
MboII GAAGA 2 cut(s) 188, 506
MhlI GDGCHC 1 cut(s) 573
MlsI TGGCCA 1 cut(s) 320
MluCI AATT 2 cut(s) 353, 688
MluNI TGGCCA 1 cut(s) 320
MlyI GAGTC 1 cut(s) 431
MmeI TCCRAC 3 cut(s) 35, 542, 566
MnlI CCTC 5 cut(s) 150, 233, 522, 594, 632
Mox20I TGGCCA 1 cut(s) 320
MscI TGGCCA 1 cut(s) 320
MseI TTAA 1 cut(s) 618
Msp20I TGGCCA 1 cut(s) 320
MspA1I CMGCKG 1 cut(s) 316
MspR9I CCNGG 6 cut(s) 59, 69, 171, 192, 322, 695
MvaI CCWGG 6 cut(s) 59, 69, 171, 192, 322, 695
MwoI GCNNNNNNNGC 3 cut(s) 71, 134, 322
NdeII GATC 3 cut(s) 197, 298, 502
NlaIII CATG 3 cut(s) 257, 554, 711
NlaIV GGNNCC 3 cut(s) 138, 221, 326
NmeAIII GCCGAG 1 cut(s) 113
NspI RCATGY 1 cut(s) 554
PaeR7I CTCGAG 1 cut(s) 585
PagI TCATGA 1 cut(s) 253
PciI ACATGT 1 cut(s) 550
PfeI GAWTC 2 cut(s) 341, 602
PfoI TCCNGGA 3 cut(s) 57, 67, 693
PkrI GCNGC 3 cut(s) 315, 631, 634
PleI GAGTC 1 cut(s) 430
PpsI GAGTC 1 cut(s) 430
PscI ACATGT 1 cut(s) 550
Psp6I CCWGG 6 cut(s) 57, 67, 169, 190, 320, 693
PspGI CCWGG 6 cut(s) 57, 67, 169, 190, 320, 693
PspN4I GGNNCC 3 cut(s) 138, 221, 326
PspXI VCTCGAGB 1 cut(s) 585
PstI CTGCAG 1 cut(s) 147
PvuII CAGCTG 1 cut(s) 316
RsaI GTAC 1 cut(s) 362
RsaNI GTAC 1 cut(s) 361
SaqAI TTAA 1 cut(s) 618
SatI GCNGC 3 cut(s) 314, 630, 633
Sau3AI GATC 3 cut(s) 197, 298, 502
ScaI AGTACT 1 cut(s) 362
SchI GAGTC 1 cut(s) 431
ScrFI CCNGG 6 cut(s) 59, 69, 171, 192, 322, 695
SduI GDGCHC 1 cut(s) 573
SfaNI GCATC 1 cut(s) 235
SfcI CTRYAG 4 cut(s) 85, 143, 237, 306
Sfr274I CTCGAG 1 cut(s) 585
SlaI CTCGAG 1 cut(s) 585
SmlI CTYRAG 1 cut(s) 585
SmoI CTYRAG 1 cut(s) 585
Sse9I AATT 2 cut(s) 353, 688
SsiI CCGC 2 cut(s) 629, 663
SspMI CTAG 1 cut(s) 701
StyD4I CCNGG 6 cut(s) 57, 67, 169, 190, 320, 693
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 1 cut(s) 523
TaiI ACGT 1 cut(s) 655
TaqI TCGA 3 cut(s) 301, 555, 586
TasI AATT 2 cut(s) 353, 688
TatI WGTACW 1 cut(s) 360
TauI GCSGC 1 cut(s) 632
TfiI GAWTC 2 cut(s) 341, 602
Tru1I TTAA 1 cut(s) 618
Tru9I TTAA 1 cut(s) 618
TscAI CASTG 1 cut(s) 601
TseI GCWGC 2 cut(s) 313, 632
TspDTI ATGAA 2 cut(s) 195, 435
TspGWI ACGGA 1 cut(s) 420
TspRI CASTG 1 cut(s) 601
XapI RAATTY 2 cut(s) 353, 688
XceI RCATGY 1 cut(s) 554
XhoI CTCGAG 1 cut(s) 585
XspI CTAG 1 cut(s) 701
ZrmI AGTACT 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.