Rmu_sc0001192.1_g000031

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001192.1
OG0014867
Physical Location & Seq
Reverse (-)
126207 .. 127441
1235 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 768 bp
atgtggcaagaattggcttgcggaaattatctgaactcccatgatggtagtcatcagggggaggtgcagacatcagagggagatgtctacatgttcgtctctaatcgtgaagggtgtgttgtgctctttttccccttcgaccgagcggaggcaatccaatccctctctgccatcctcctctccctcatctaccgcctctaccgccgctcgatcgacgcggccgtctgcatccccgagatcttccgatccgagccggagctagcccagacggaattggctccggtgctattcgaggagctcttcctcatccacctcctcctggttctccagtggtacaatcgccggcgatcgcagattctgccgtcgctgtccccaaatgtcagccacaaaatcaacgacatttcgatcaacgacatgtcattggattttccggaggggtcgaatttgctatcgaagatgagtgggaaccagacggcggggctgaaggagatggcgagcagccacaagagggttttggatgagaattgtagggttttcaatgagcatttcagggaggttttggagaataaagatgaaggaagagtgattgatccatcggctgtggtgttggaattgagtgaaggaagtgatgagttcctatatgatgattggagcaccggagaagatgaaggtctggattcaactggtgatggtggtgctggagatggtggccggaagtggtggtcttcgatgatggcagaggaagagaagaagaagaagagaggttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box WD40

Protein Analysis

255

Amino Acids

28.8

Weight (kDa)

4.71

Isoelectric Point (pI)

59.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 54 - 194 1.8e-13 E3 ubiquitin-protein ligase LIN-like, ARM repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 221
AccBSI CCGCTC 2 cut(s) 146, 207
AccI GTMKAC 1 cut(s) 87
AccII CGCG 1 cut(s) 218
AccIII TCCGGA 1 cut(s) 430
AciI CCGC 7 cut(s) 21, 146, 193, 202, 205, 218, 476
AclWI GGATC 2 cut(s) 240, 584
AcoI YGGCCR 2 cut(s) 219, 709
AcsI RAATTY 1 cut(s) 442
AcuI CTGAAG 1 cut(s) 503
AfaI GTAC 1 cut(s) 335
AfiI CCNNNNNNNGG 4 cut(s) 148, 319, 475, 508
AflIII ACRYGT 2 cut(s) 90, 414
AgsI TTSAA 2 cut(s) 538, 681
AjnI CCWGG 1 cut(s) 318
AluBI AGCT 2 cut(s) 259, 298
AluI AGCT 2 cut(s) 259, 298
Alw21I GWGCWC 3 cut(s) 126, 300, 656
Alw26I GTCTC 1 cut(s) 103
AlwI GGATC 2 cut(s) 240, 584
AlwNI CAGNNNCTG 1 cut(s) 358
Ama87I CYCGRG 1 cut(s) 233
Aor13HI TCCGGA 1 cut(s) 430
AoxI GGCC 2 cut(s) 219, 709
ApeKI GCWGC 1 cut(s) 498
ApoI RAATTY 1 cut(s) 442
AsiSI GCGATCGC 1 cut(s) 350
AsuHPI GGTGA 1 cut(s) 698
AsuNHI GCTAGC 1 cut(s) 259
AvaI CYCGRG 1 cut(s) 233
BanII GRGCYC 1 cut(s) 300
BbsI GAAGAC 1 cut(s) 717
Bbv12I GWGCWC 3 cut(s) 126, 300, 656
BbvI GCAGC 1 cut(s) 510
BccI CCATC 7 cut(s) 38, 179, 484, 601, 683, 698, 727
BceAI ACGGC 3 cut(s) 206, 346, 489
BciT130I CCWGG 1 cut(s) 320
BcoDI GTCTC 1 cut(s) 103
BfaI CTAG 1 cut(s) 260
BglII AGATCT 1 cut(s) 237
BisI GCNGC 3 cut(s) 205, 219, 499
BlsI GCNGC 3 cut(s) 206, 220, 500
Bme1390I CCNGG 1 cut(s) 320
BmeT110I CYCGRG 1 cut(s) 233
BmiI GGNNCC 2 cut(s) 279, 467
BmrFI CCNGG 1 cut(s) 320
BmsI GCATC 1 cut(s) 237
BmtI GCTAGC 1 cut(s) 263
BpiI GAAGAC 1 cut(s) 717
BpmI CTGGAG 2 cut(s) 311, 720
BsaWI WCCGGW 3 cut(s) 280, 430, 656
Bsc4I CCNNNNNNNGG 4 cut(s) 148, 319, 475, 508
Bse118I RCCGGY 1 cut(s) 342
Bse1I ACTGG 2 cut(s) 328, 688
BseAI TCCGGA 1 cut(s) 430
BseBI CCWGG 1 cut(s) 320
BseGI GGATG 4 cut(s) 171, 228, 306, 523
BseLI CCNNNNNNNGG 4 cut(s) 148, 319, 475, 508
BseNI ACTGG 2 cut(s) 328, 688
BseRI GAGGAG 3 cut(s) 167, 305, 308
BseX3I CGGCCG 1 cut(s) 219
BseXI GCAGC 1 cut(s) 510
BsgI GTGCAG 1 cut(s) 86
Bsh1236I CGCG 1 cut(s) 218
Bsh1285I CGRYCG 4 cut(s) 142, 213, 222, 350
BshFI GGCC 2 cut(s) 221, 711
BsiEI CGRYCG 4 cut(s) 142, 213, 222, 350
BsiHKAI GWGCWC 3 cut(s) 126, 300, 656
BsiHKCI CYCGRG 1 cut(s) 233
BsiSI CCGG 6 cut(s) 254, 281, 343, 431, 657, 712
BslFI GGGAC 1 cut(s) 355
BslI CCNNNNNNNGG 4 cut(s) 148, 319, 475, 508
BsmAI GTCTC 1 cut(s) 103
BsmBI CGTCTC 1 cut(s) 103
BsmFI GGGAC 1 cut(s) 355
BsnI GGCC 2 cut(s) 221, 711
BsoBI CYCGRG 1 cut(s) 233
Bsp1286I GDGCHC 3 cut(s) 126, 300, 656
Bsp13I TCCGGA 1 cut(s) 430
Bsp143I GATC 6 cut(s) 210, 237, 245, 347, 405, 589
BspACI CCGC 7 cut(s) 21, 146, 193, 202, 205, 218, 476
BspANI GGCC 2 cut(s) 221, 711
BspEI TCCGGA 1 cut(s) 430
BspFNI CGCG 1 cut(s) 218
BspLI GGNNCC 2 cut(s) 279, 467
BspOI GCTAGC 1 cut(s) 263
BspPI GGATC 2 cut(s) 240, 584
BspQI GCTCTTC 1 cut(s) 305
BsrBI CCGCTC 2 cut(s) 146, 207
BsrFI RCCGGY 1 cut(s) 342
BsrI ACTGG 2 cut(s) 328, 688
BssAI RCCGGY 1 cut(s) 342
BssMI GATC 6 cut(s) 210, 237, 245, 347, 405, 589
Bst2UI CCWGG 1 cut(s) 320
Bst6I CTCTTC 4 cut(s) 305, 574, 738, 752
BstAPI GCANNNNNTGC 1 cut(s) 358
BstC8I GCNNGC 4 cut(s) 19, 261, 344, 496
BstF5I GGATG 4 cut(s) 171, 228, 306, 523
BstFNI CGCG 1 cut(s) 218
BstKTI GATC 6 cut(s) 213, 240, 248, 350, 408, 592
BstMAI GTCTC 1 cut(s) 103
BstMBI GATC 6 cut(s) 210, 237, 245, 347, 405, 589
BstMCI CGRYCG 4 cut(s) 142, 213, 222, 350
BstMWI GCNNNNNNNGC 2 cut(s) 201, 358
BstNI CCWGG 1 cut(s) 320
BstNSI RCATGY 2 cut(s) 94, 418
BstSCI CCNGG 1 cut(s) 318
BstUI CGCG 1 cut(s) 218
BstV1I GCAGC 1 cut(s) 510
BstV2I GAAGAC 1 cut(s) 717
BstX2I RGATCY 1 cut(s) 237
BstYI RGATCY 1 cut(s) 237
BstZI CGGCCG 1 cut(s) 219
BsuRI GGCC 2 cut(s) 221, 711
BtsCI GGATG 4 cut(s) 171, 228, 306, 523
BtsIMutI CAGTG 1 cut(s) 335
Cac8I GCNNGC 4 cut(s) 19, 261, 344, 496
CaiI CAGNNNCTG 1 cut(s) 358
Cfr10I RCCGGY 1 cut(s) 342
CseI GACGC 1 cut(s) 224
Csp6I GTAC 1 cut(s) 334
CviAII CATG 3 cut(s) 41, 91, 415
CviQI GTAC 1 cut(s) 334
DpnI GATC 6 cut(s) 212, 239, 247, 349, 407, 591
DpnII GATC 6 cut(s) 210, 237, 245, 347, 405, 589
DrdI GACNNNNNNGTC 1 cut(s) 221
DseDI GACNNNNNNGTC 1 cut(s) 221
EaeI YGGCCR 2 cut(s) 219, 709
EagI CGGCCG 1 cut(s) 219
Eam1104I CTCTTC 4 cut(s) 305, 574, 738, 752
EarI CTCTTC 4 cut(s) 305, 574, 738, 752
Ecl136II GAGCTC 1 cut(s) 298
EclXI CGGCCG 1 cut(s) 219
Eco24I GRGCYC 1 cut(s) 300
Eco52I CGGCCG 1 cut(s) 219
Eco53kI GAGCTC 1 cut(s) 298
Eco57I CTGAAG 1 cut(s) 503
Eco88I CYCGRG 1 cut(s) 233
EcoICRI GAGCTC 1 cut(s) 298
EcoRII CCWGG 1 cut(s) 318
EcoT38I GRGCYC 1 cut(s) 300
Esp3I CGTCTC 1 cut(s) 103
FaeI CATG 3 cut(s) 44, 94, 418
FaiI YATR 5 cut(s) 42, 92, 416, 640, 642
FaqI GGGAC 1 cut(s) 355
FatI CATG 3 cut(s) 40, 90, 414
FauI CCCGC 1 cut(s) 469
FblI GTMKAC 1 cut(s) 87
Fnu4HI GCNGC 3 cut(s) 205, 219, 499
FokI GGATG 4 cut(s) 158, 215, 293, 530
FriOI GRGCYC 1 cut(s) 300
Fsp4HI GCNGC 3 cut(s) 205, 219, 499
FspBI CTAG 1 cut(s) 260
GluI GCNGC 3 cut(s) 205, 219, 499
GsuI CTGGAG 2 cut(s) 311, 720
HaeIII GGCC 2 cut(s) 221, 711
HapII CCGG 6 cut(s) 254, 281, 343, 431, 657, 712
HgaI GACGC 1 cut(s) 224
Hin1II CATG 3 cut(s) 44, 94, 418
HinfI GANTC 2 cut(s) 355, 677
HpaII CCGG 6 cut(s) 254, 281, 343, 431, 657, 712
HphI GGTGA 1 cut(s) 698
Hpy166II GTNNAC 1 cut(s) 88
Hpy188I TCNGA 4 cut(s) 33, 76, 245, 250
Hpy188III TCNNGA 3 cut(s) 107, 431, 674
Hpy8I GTNNAC 1 cut(s) 88
Hpy99I CGWCG 2 cut(s) 218, 367
HpyAV CCTTC 6 cut(s) 104, 145, 478, 569, 614, 662
HpyCH4V TGCA 2 cut(s) 67, 228
HpyF10VI GCNNNNNNNGC 2 cut(s) 201, 358
Hsp92II CATG 3 cut(s) 44, 94, 418
Kpn2I TCCGGA 1 cut(s) 430
KroI GCCGGC 1 cut(s) 342
KroNI GCCGGC 1 cut(s) 344
Kzo9I GATC 6 cut(s) 210, 237, 245, 347, 405, 589
LguI GCTCTTC 1 cut(s) 305
LmnI GCTCC 4 cut(s) 256, 283, 295, 651
Lsp1109I GCAGC 1 cut(s) 510
LweI GCATC 1 cut(s) 237
MaeI CTAG 1 cut(s) 260
MalI GATC 6 cut(s) 212, 239, 247, 349, 407, 591
MbiI CCGCTC 2 cut(s) 146, 207
MboI GATC 6 cut(s) 210, 237, 245, 347, 405, 589
MflI RGATCY 1 cut(s) 237
MhlI GDGCHC 3 cut(s) 126, 300, 656
MluCI AATT 6 cut(s) 11, 25, 272, 442, 523, 611
MmeI TCCRAC 1 cut(s) 588
MreI CGCCGGCG 1 cut(s) 342
MroI TCCGGA 1 cut(s) 430
MroNI GCCGGC 1 cut(s) 342
MspI CCGG 6 cut(s) 254, 281, 343, 431, 657, 712
MspR9I CCNGG 1 cut(s) 320
MvaI CCWGG 1 cut(s) 320
MvnI CGCG 1 cut(s) 218
MwoI GCNNNNNNNGC 2 cut(s) 201, 358
NaeI GCCGGC 1 cut(s) 344
NdeII GATC 6 cut(s) 210, 237, 245, 347, 405, 589
NgoMIV GCCGGC 1 cut(s) 342
NheI GCTAGC 1 cut(s) 259
NlaIII CATG 3 cut(s) 44, 94, 418
NlaIV GGNNCC 2 cut(s) 279, 467
NspI RCATGY 2 cut(s) 94, 418
PciI ACATGT 2 cut(s) 90, 414
PciSI GCTCTTC 1 cut(s) 305
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PdiI GCCGGC 1 cut(s) 344
PfeI GAWTC 2 cut(s) 355, 677
PkrI GCNGC 3 cut(s) 206, 220, 500
Ple19I CGATCG 2 cut(s) 213, 350
PscI ACATGT 2 cut(s) 90, 414
Psp124BI GAGCTC 1 cut(s) 300
Psp6I CCWGG 1 cut(s) 318
PspGI CCWGG 1 cut(s) 318
PspN4I GGNNCC 2 cut(s) 279, 467
PstNI CAGNNNCTG 1 cut(s) 358
PsuI RGATCY 1 cut(s) 237
PvuI CGATCG 2 cut(s) 213, 350
RgaI GCGATCGC 1 cut(s) 350
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
SacI GAGCTC 1 cut(s) 300
SapI GCTCTTC 1 cut(s) 305
SatI GCNGC 3 cut(s) 205, 219, 499
Sau3AI GATC 6 cut(s) 210, 237, 245, 347, 405, 589
ScrFI CCNGG 1 cut(s) 320
SduI GDGCHC 3 cut(s) 126, 300, 656
SetI ASST 7 cut(s) 66, 261, 300, 315, 558, 673, 766
SfaAI GCGATCGC 1 cut(s) 350
SfaNI GCATC 1 cut(s) 237
SgfI GCGATCGC 1 cut(s) 350
SgrAI CRCCGGYG 1 cut(s) 342
Sse9I AATT 6 cut(s) 11, 25, 272, 442, 523, 611
SsiI CCGC 7 cut(s) 21, 146, 193, 202, 205, 218, 476
SspMI CTAG 1 cut(s) 260
SstI GAGCTC 1 cut(s) 300
StyD4I CCNGG 1 cut(s) 318
TaqI TCGA 8 cut(s) 138, 209, 213, 291, 404, 440, 452, 728
TaqII GACCGA 1 cut(s) 156
TasI AATT 6 cut(s) 11, 25, 272, 442, 523, 611
TauI GCSGC 2 cut(s) 207, 221
TfiI GAWTC 2 cut(s) 355, 677
TscAI CASTG 1 cut(s) 335
TseI GCWGC 1 cut(s) 498
TspDTI ATGAA 2 cut(s) 588, 681
TspGWI ACGGA 1 cut(s) 284
TspRI CASTG 1 cut(s) 335
XapI RAATTY 1 cut(s) 442
XceI RCATGY 2 cut(s) 94, 418
XmiI GTMKAC 1 cut(s) 87
XspI CTAG 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.