Rmu_sc0001218.1_g000051

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001218.1
OG0016470
Physical Location & Seq
Forward (+)
211814 .. 213845
2032 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1467 bp
atgtgtcaggttaccttgaaggagaaggttgctattggagaactaaacactagcggaagtgatgatgtattgacgttggctttggggactcctgagcatcctggaagagtgagaggcgtgggaggttatgtgaacccctccatttacttccacttccctaaacgtagaaaggagagcatccatgaggcggtgaaaataattgcagaagaaagggcattttggattgctaaatttgcagagctagaagcaaaggtagatggaaggcgtagtgggacaagcgacaaaagtaatctgcatggttctgtgggtagttgttcacaaaccgatattcccggtgttggtgaagtgagatgtgatgtgatggaagaagcagcgactcgttttgatgggaaatcagttcaaaaagttctagctgcattggaagaaaagctggtgcttaaaaaggtttccaagaagaaggagaaggatgccaaatctgtccccattaaccacaaacataatgaccagggcaagttggctgaggtggtagataatgcaaaactggaagaagatcaagctcaggtgcatgatgaccctatagggtatatagatttgacaacaaagcaagaaaaatcaaaggttatgggagttgagattgatgccaagttggcattagggtcaattgaccatattgtagctctagctactgttatggaatgcgatgactcctctcaaacttgtcatggttatccactaggggataataatgtgcgagtttcagtctattgtgttgtagatgagaaagccttggttccatttcctgtggccgatgaaatactaactgttaggcaagccatggggagctgggtagcatggccaaaagagctggtcataatgtcacatgacaagaaatttaccaaggagaaagctgagaagaggaagagaaaaaaattggtactggaagaagacgaaaatgctaaggtactaattagcttaaagtctttagcaccttccttgccagctccattgaagatactttgcaattggggtgaagaggcatttaaagatgggcgtgctatcacgttcgaaatggagtctgaagtatttgggtatcaacgaaagacgtttatactaggatcagatgttgaaagaattgctagcatgagggaagttactggaaactgcatagtggtataccagaggtaccttcttgatttattgactcgatataaaatgctggatatggttgcttttgttgacccttcaatgatcggtgcaatccggtacggggatggtcatgcccagtcccagcatatcaaggataggcttttaacggcaaaaccaaaccagattttcttgctgccttataacgcagataatcattggatgcttactgttatgaatccagaggaaaacactgtctatttcatggatccgttgaagaggcggttacccactggagaatggagatcaattgttgatacgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

488

Amino Acids

54.66

Weight (kDa)

6.39

Isoelectric Point (pI)

31.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8039 PF26133 219 - 292 2.9e-12 Domain of unknown function (DUF8039)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1347
Acc65I GGTACC 1 cut(s) 1182
AccB1I GGYRCC 1 cut(s) 1182
AccI GTMKAC 1 cut(s) 1173
AciI CCGC 3 cut(s) 54, 188, 1426
AclWI GGATC 3 cut(s) 1123, 1406, 1419
AcoI YGGCCR 2 cut(s) 804, 854
AcsI RAATTY 2 cut(s) 230, 890
AcuI CTGAAG 1 cut(s) 1098
AfaI GTAC 4 cut(s) 936, 963, 1184, 1265
AfiI CCNNNNNNNGG 3 cut(s) 187, 338, 1267
AgsI TTSAA 6 cut(s) 19, 401, 1009, 1127, 1245, 1420
AjnI CCWGG 2 cut(s) 100, 504
AlwI GGATC 3 cut(s) 1123, 1406, 1419
AoxI GGCC 2 cut(s) 804, 854
ApeKI GCWGC 3 cut(s) 371, 413, 1339
ApoI RAATTY 2 cut(s) 230, 890
ArsI GACNNNNNNTTYG 2 cut(s) 64, 96
Asp718I GGTACC 1 cut(s) 1182
AsuC2I CCSGG 1 cut(s) 333
AsuHPI GGTGA 3 cut(s) 202, 353, 1040
AsuII TTCGAA 1 cut(s) 1065
AsuNHI GCTAGC 1 cut(s) 1136
BalI TGGCCA 1 cut(s) 856
BamHI GGATCC 1 cut(s) 1411
BanI GGYRCC 1 cut(s) 1182
BbsI GAAGAC 1 cut(s) 951
BbvCI CCTCAGC 1 cut(s) 519
BbvI GCAGC 3 cut(s) 383, 400, 1326
BccI CCATC 5 cut(s) 251, 355, 380, 1040, 1265
BceAI ACGGC 1 cut(s) 1329
BciT130I CCWGG 2 cut(s) 102, 506
BcnI CCSGG 1 cut(s) 333
BfaI CTAG 7 cut(s) 51, 242, 410, 680, 734, 1112, 1137
BfmI CTRYAG 1 cut(s) 576
BglI GCCNNNNNGGC 1 cut(s) 647
BisI GCNGC 3 cut(s) 372, 414, 1340
BlsI GCNGC 3 cut(s) 373, 415, 1341
Bme1390I CCNGG 3 cut(s) 102, 333, 506
BmiI GGNNCC 3 cut(s) 792, 1184, 1413
BmrFI CCNGG 3 cut(s) 102, 333, 506
BmrI ACTGGG 1 cut(s) 1276
BmsI GCATC 5 cut(s) 106, 186, 457, 628, 1356
BmtI GCTAGC 1 cut(s) 1140
BmuI ACTGGG 1 cut(s) 1276
BpiI GAAGAC 1 cut(s) 951
BpmI CTGGAG 1 cut(s) 1458
Bpu10I CCTNAGC 4 cut(s) 93, 519, 558, 957
Bpu14I TTCGAA 1 cut(s) 1065
BpuMI CCSGG 1 cut(s) 333
BsaAI YACGTR 1 cut(s) 1464
BsaJI CCNNGG 4 cut(s) 505, 786, 834, 897
BsaWI WCCGGW 1 cut(s) 1260
BsaXI ACNNNNNCTCC 2 cut(s) 114, 144
Bsc4I CCNNNNNNNGG 3 cut(s) 187, 338, 1267
Bse1I ACTGG 5 cut(s) 546, 942, 1159, 1282, 1441
BseBI CCWGG 2 cut(s) 102, 506
BseDI CCNNGG 4 cut(s) 505, 786, 834, 897
BseGI GGATG 5 cut(s) 97, 177, 472, 1276, 1371
BseLI CCNNNNNNNGG 3 cut(s) 187, 338, 1267
BseMII CTCAG 4 cut(s) 84, 510, 572, 900
BseNI ACTGG 5 cut(s) 546, 942, 1159, 1282, 1441
BseRI GAGGAG 1 cut(s) 697
BseXI GCAGC 3 cut(s) 383, 400, 1326
BseYI CCCAGC 2 cut(s) 843, 1287
BshFI GGCC 2 cut(s) 806, 856
BshNI GGYRCC 1 cut(s) 1182
BsiSI CCGG 2 cut(s) 333, 1261
BslFI GGGAC 4 cut(s) 100, 286, 464, 1270
BslI CCNNNNNNNGG 3 cut(s) 187, 338, 1267
BsmFI GGGAC 4 cut(s) 100, 286, 464, 1270
BsmI GAATGC 1 cut(s) 701
BsnI GGCC 2 cut(s) 806, 856
Bsp119I TTCGAA 1 cut(s) 1065
Bsp143I GATC 5 cut(s) 550, 1115, 1248, 1411, 1448
Bsp19I CCATGG 1 cut(s) 834
BspACI CCGC 3 cut(s) 54, 188, 1426
BspANI GGCC 2 cut(s) 806, 856
BspCNI CTCAG 4 cut(s) 85, 511, 571, 901
BspLI GGNNCC 3 cut(s) 792, 1184, 1413
BspOI GCTAGC 1 cut(s) 1140
BspPI GGATC 3 cut(s) 1123, 1406, 1419
BspT104I TTCGAA 1 cut(s) 1065
BspT107I GGYRCC 1 cut(s) 1182
BsrI ACTGG 5 cut(s) 546, 942, 1159, 1282, 1441
BssECI CCNNGG 4 cut(s) 505, 786, 834, 897
BssMI GATC 5 cut(s) 550, 1115, 1248, 1411, 1448
BssNAI GTATAC 1 cut(s) 1174
BssT1I CCWWGG 3 cut(s) 786, 834, 897
Bst1107I GTATAC 1 cut(s) 1174
Bst2UI CCWGG 2 cut(s) 102, 506
Bst4CI ACNGT 4 cut(s) 688, 823, 1375, 1399
Bst6I CTCTTC 5 cut(s) 100, 908, 914, 1026, 1415
BstBAI YACGTR 1 cut(s) 1464
BstBI TTCGAA 1 cut(s) 1065
BstC8I GCNNGC 4 cut(s) 831, 999, 1053, 1138
BstDEI CTNAG 5 cut(s) 93, 519, 558, 909, 957
BstDSI CCRYGG 1 cut(s) 834
BstEII GGTNACC 2 cut(s) 10, 1428
BstF5I GGATG 5 cut(s) 97, 177, 472, 1276, 1371
BstKTI GATC 5 cut(s) 553, 1118, 1251, 1414, 1451
BstMBI GATC 5 cut(s) 550, 1115, 1248, 1411, 1448
BstMWI GCNNNNNNNGC 3 cut(s) 233, 647, 862
BstNI CCWGG 2 cut(s) 102, 506
BstPI GGTNACC 2 cut(s) 10, 1428
BstSCI CCNGG 3 cut(s) 100, 331, 504
BstSFI CTRYAG 1 cut(s) 576
BstSNI TACGTA 1 cut(s) 1464
BstV1I GCAGC 3 cut(s) 383, 400, 1326
BstV2I GAAGAC 1 cut(s) 951
BstX2I RGATCY 1 cut(s) 1411
BstYI RGATCY 1 cut(s) 1411
BstZ17I GTATAC 1 cut(s) 1174
BsuRI GGCC 2 cut(s) 806, 856
BtgI CCRYGG 1 cut(s) 834
BtgZI GCGATG 1 cut(s) 714
BtsCI GGATG 5 cut(s) 97, 177, 472, 1276, 1371
BtsIMutI CAGTG 2 cut(s) 1395, 1434
Cac8I GCNNGC 4 cut(s) 831, 999, 1053, 1138
Csp6I GTAC 4 cut(s) 935, 962, 1183, 1264
CviQI GTAC 4 cut(s) 935, 962, 1183, 1264
DdeI CTNAG 5 cut(s) 93, 519, 558, 909, 957
DpnI GATC 5 cut(s) 552, 1117, 1250, 1413, 1450
DpnII GATC 5 cut(s) 550, 1115, 1248, 1411, 1448
DraI TTTAAA 1 cut(s) 1042
EaeI YGGCCR 2 cut(s) 804, 854
Eam1104I CTCTTC 5 cut(s) 100, 908, 914, 1026, 1415
EarI CTCTTC 5 cut(s) 100, 908, 914, 1026, 1415
Eco105I TACGTA 1 cut(s) 1464
Eco130I CCWWGG 3 cut(s) 786, 834, 897
Eco57I CTGAAG 1 cut(s) 1098
Eco91I GGTNACC 2 cut(s) 10, 1428
EcoO65I GGTNACC 2 cut(s) 10, 1428
EcoRII CCWGG 2 cut(s) 100, 504
EcoT14I CCWWGG 3 cut(s) 786, 834, 897
ErhI CCWWGG 3 cut(s) 786, 834, 897
FaqI GGGAC 4 cut(s) 100, 286, 464, 1270
FblI GTMKAC 1 cut(s) 1173
Fnu4HI GCNGC 3 cut(s) 372, 414, 1340
FokI GGATG 5 cut(s) 84, 164, 479, 1283, 1378
Fsp4HI GCNGC 3 cut(s) 372, 414, 1340
FspBI CTAG 7 cut(s) 51, 242, 410, 680, 734, 1112, 1137
GluI GCNGC 3 cut(s) 372, 414, 1340
GsaI CCCAGC 2 cut(s) 847, 1291
GsuI CTGGAG 1 cut(s) 1458
HaeIII GGCC 2 cut(s) 806, 856
HapII CCGG 2 cut(s) 333, 1261
HincII GTYRAC 1 cut(s) 1237
HindII GTYRAC 1 cut(s) 1237
HinfI GANTC 6 cut(s) 88, 376, 704, 1073, 1201, 1381
HpaII CCGG 2 cut(s) 333, 1261
HphI GGTGA 3 cut(s) 202, 353, 1040
Hpy166II GTNNAC 4 cut(s) 133, 317, 1174, 1237
Hpy188I TCNGA 2 cut(s) 1078, 1120
Hpy188III TCNNGA 3 cut(s) 92, 1190, 1385
Hpy8I GTNNAC 4 cut(s) 133, 317, 1174, 1237
HpyAV CCTTC 8 cut(s) 13, 19, 255, 451, 457, 999, 1196, 1251
HpyCH4III ACNGT 4 cut(s) 688, 823, 1375, 1399
HpyCH4IV ACGT 5 cut(s) 74, 163, 1061, 1103, 1463
HpyCH4V TGCA 9 cut(s) 203, 236, 295, 416, 536, 565, 1020, 1164, 1256
HpyF10VI GCNNNNNNNGC 3 cut(s) 233, 647, 862
HpyF3I CTNAG 5 cut(s) 93, 519, 558, 909, 957
HpySE526I ACGT 5 cut(s) 74, 163, 1061, 1103, 1463
KpnI GGTACC 1 cut(s) 1186
Kzo9I GATC 5 cut(s) 550, 1115, 1248, 1411, 1448
LmnI GCTCC 2 cut(s) 840, 1006
Lsp1109I GCAGC 3 cut(s) 383, 400, 1326
LweI GCATC 5 cut(s) 106, 186, 457, 628, 1356
MaeI CTAG 7 cut(s) 51, 242, 410, 680, 734, 1112, 1137
MaeII ACGT 5 cut(s) 74, 163, 1061, 1103, 1463
MaeIII GTNAC 4 cut(s) 10, 876, 1150, 1428
MalI GATC 5 cut(s) 552, 1117, 1250, 1413, 1450
MboI GATC 5 cut(s) 550, 1115, 1248, 1411, 1448
MfeI CAATTG 3 cut(s) 660, 1021, 1452
MflI RGATCY 1 cut(s) 1411
MlsI TGGCCA 1 cut(s) 856
MluCI AATT 9 cut(s) 198, 230, 660, 890, 929, 966, 1021, 1131, 1452
MluNI TGGCCA 1 cut(s) 856
MlyI GAGTC 5 cut(s) 82, 370, 698, 1082, 1195
Mox20I TGGCCA 1 cut(s) 856
MscI TGGCCA 1 cut(s) 856
MseI TTAA 5 cut(s) 438, 486, 974, 1041, 1310
Msp20I TGGCCA 1 cut(s) 856
MspI CCGG 2 cut(s) 333, 1261
MspR9I CCNGG 3 cut(s) 102, 333, 506
MunI CAATTG 3 cut(s) 660, 1021, 1452
Mva1269I GAATGC 1 cut(s) 701
MvaI CCWGG 2 cut(s) 102, 506
MwoI GCNNNNNNNGC 3 cut(s) 233, 647, 862
NciI CCSGG 1 cut(s) 333
NcoI CCATGG 1 cut(s) 834
NdeII GATC 5 cut(s) 550, 1115, 1248, 1411, 1448
NheI GCTAGC 1 cut(s) 1136
NlaIV GGNNCC 3 cut(s) 792, 1184, 1413
NmuCI GTSAC 1 cut(s) 876
NspV TTCGAA 1 cut(s) 1065
PctI GAATGC 1 cut(s) 701
PfeI GAWTC 1 cut(s) 1381
PfoI TCCNGGA 1 cut(s) 100
PkrI GCNGC 3 cut(s) 373, 415, 1341
PleI GAGTC 5 cut(s) 82, 370, 698, 1081, 1195
PpsI GAGTC 5 cut(s) 82, 370, 698, 1081, 1195
Ppu21I YACGTR 1 cut(s) 1464
PsiI TTATAA 1 cut(s) 1347
Psp6I CCWGG 2 cut(s) 100, 504
PspEI GGTNACC 2 cut(s) 10, 1428
PspFI CCCAGC 2 cut(s) 843, 1287
PspGI CCWGG 2 cut(s) 100, 504
PspN4I GGNNCC 3 cut(s) 792, 1184, 1413
PsuI RGATCY 1 cut(s) 1411
RsaI GTAC 4 cut(s) 936, 963, 1184, 1265
RsaNI GTAC 4 cut(s) 935, 962, 1183, 1264
SaqAI TTAA 5 cut(s) 438, 486, 974, 1041, 1310
SatI GCNGC 3 cut(s) 372, 414, 1340
Sau3AI GATC 5 cut(s) 550, 1115, 1248, 1411, 1448
SchI GAGTC 5 cut(s) 82, 370, 698, 1082, 1195
ScrFI CCNGG 3 cut(s) 102, 333, 506
SfaNI GCATC 5 cut(s) 106, 186, 457, 628, 1356
SfcI CTRYAG 1 cut(s) 576
SfuI TTCGAA 1 cut(s) 1065
SnaBI TACGTA 1 cut(s) 1464
Sse9I AATT 9 cut(s) 198, 230, 660, 890, 929, 966, 1021, 1131, 1452
SsiI CCGC 3 cut(s) 54, 188, 1426
SspMI CTAG 7 cut(s) 51, 242, 410, 680, 734, 1112, 1137
StyD4I CCNGG 3 cut(s) 100, 331, 504
StyI CCWWGG 3 cut(s) 786, 834, 897
TaaI ACNGT 4 cut(s) 688, 823, 1375, 1399
TaiI ACGT 5 cut(s) 77, 166, 1064, 1106, 1466
TaqI TCGA 2 cut(s) 1065, 1204
TasI AATT 9 cut(s) 198, 230, 660, 890, 929, 966, 1021, 1131, 1452
TfiI GAWTC 1 cut(s) 1381
Tru1I TTAA 5 cut(s) 438, 486, 974, 1041, 1310
Tru9I TTAA 5 cut(s) 438, 486, 974, 1041, 1310
TscAI CASTG 2 cut(s) 1402, 1441
TseFI GTSAC 1 cut(s) 876
TseI GCWGC 3 cut(s) 371, 413, 1339
Tsp45I GTSAC 1 cut(s) 876
TspDTI ATGAA 3 cut(s) 825, 1394, 1396
TspGWI ACGGA 1 cut(s) 1404
TspRI CASTG 2 cut(s) 1402, 1441
XapI RAATTY 2 cut(s) 230, 890
XmiI GTMKAC 1 cut(s) 1173
XspI CTAG 7 cut(s) 51, 242, 410, 680, 734, 1112, 1137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.