Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0001252.1

Orthogroup ID

OG0004385

Physical Location & Seq

Genomic Coordinates Reverse (-)

5120 .. 11246

Gene Structure 6127 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2994 bp

atgtcacatatcaaaccaacccaaaccgccatttttcgttctctgaagaagctctgcaactcaaaacacccagtgaggaagcctataaatagggacacaaagacagtgaaaatgtcatccacagtgaaaggtttactcttgaggacttcaccggaggtagcttatagacacagaatgttcaacgatcgttctttcctcaacggcgtgaacggtggctccacccgagccggccagacccggcatcagcctcacgttgctaccataacaaatgggatcaccaaccccagtcctaagaaaaatgagctgcagagccagatcggagcccagcctatgatgtcaccagttttggacccgagtactccaacaacgcacaagagggtttttacttttgggaagggaaggagtgaaggaaacaagggcatgaagtcactgttgggagggaagggtgcaaacctggctgaaatggctagcatcgggctatccgtgccacctgggcttactatctcaacagaagcatgccaagagtatcaggagaatggtaaagaattaccggaaggtttgtgggaggagatactagaaggcttggattatgtgcagaagactatgggagctactcttggtgacccttccaagcccctccttgtctctgttcgctctggtgcagcgatttctatgcctggcatgatggacactgtcctcaaccttggactgaatgatgaggtggttgctggtttagctgtaaaaagtggggagcgctttgcatatgactcatacagacgtttcctagacatgtttggagatgttgttatggaaattccacactcatcatttggggagaagttggaaaactacaaacaccaaaaaggagttgaacttgatacagagctaacagcttctgatctcaaagagctggtagaacaatacaaaagtgtctatcttgaagctacgggtcaaaaattcccttcagatccaaaacaacagctagtgttggctattaaagcagtttttaattcttgggacagcccgagggccaacaaataccggagcatcaatcagataactggtttaaagggaactgcagttaacattcaaagcatggcttttgggaacatgggaagcacttcagggacaggtgttctattcactagaaatccaagcactggtgaaaagaagctctatggggagtttctagtcaatgctcagggagaagatgtagttgccggtattaggacaccagaacctttggataccatgaaaaattgcatgccagaagcttacaaggagcttgtggagaactgtgaaattctagaaagacattacaaagatatgatggacattgagttcactgtccaagaaaatagattatggatgttgcaatgtcgtactggaaagcgtactggtaaaggtgcggtcaagatagctgtagacatggttaatgaagggcttgttgaaaagcgctccgcaatcaagatggtggaaccacaacatgttgaccaacttctacaccctcagtttgaggatccgacagcatacaaagacaaagtgcttgccactggcttgcctgcatcaccaggagcagcagtagggcaaatcgtgttcagtgcagaggacgctgaatcatggcacgcacaaggaaagaagaccattttggtaaggacggagaccagcccggaagatattgggggaatgcatgcatctgctgggatcttgactgctagaggtggcatgacatctcacgcagctgttgtcgctcgtggatggggtaaatgttgtgtttctggatgctctgatatccgagtaaatgatgatgagaaggttgttgtgattggggacactttgtttagagaaggagaatggctctccctcaatggaaacaccggtgaagtcatatcaggaaaacagccactttctcctccagctttaagcggtgatttggaaacctttatgtcttgggctgatagctttaggcgtctcaaggttatggcaaatgccgacacacctgaagatgcactaacagcaagaaacaatggtgcacaagggattggactttgcaggacagagcacatgttctttgcttcagaggaaagaattaagtcagtaagaaagatgatcatggcaaatacacttgagcagaggaaggcagcattggatcttttactaccatatcagagatctgactttgaaggaattttccgtgcaatggatggtcttccggtaaccatccgattgttagaccctccacttcatgagtttcttccagaaggtgacctagaacagattgttaaagaagtagctgcagagactgacatgactgaggatgatgtttactcgagagttgataaattaacagaagtaaacccaatgcttggtttccggggctgcaggctagggatttcgtacccagaactcacagaaatgcaagcacgtgcagtctttcaggctgcagtcttgatgagcaaccagggtgtcaaagttttccctgagataatggttcctcttgttggatcagctcaggaactgggacaccaagtgactttaatccggagtgttgcaaagaaagtgttctctgagatgggtactaccttgagtttcaaggttggaaccatgattgagattcccagagctgctctgattgcagatgagattgcaaaagaagcagagttcttctcttttggaaccaatgatctcacccagatgacctttgggtacagtagagatgatgtgggcaagttcctacctatttatttgtccaaaggcattcttcaaaatgatccatttgaggtactggatcaaagaggagttgggcagctcatcaagatggccacagaaaagggtcgtgcagctaagccaagcttaaaggttggaatatgcggtgagcatggtggggaaccttcttctgttgcatttttcgctgaggccggactagactatgtttcatgttctccattcagggtacctattgcaaggctagcagcagctcaagttgctgtctga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko00720 ko01100 ko01120 ko01200 map00620 map00710 map00720 map01100 map01120 map01200

Pfam Domains

Protein Families

PEP-utilizers PEP-utilizers_C PPDK_N

Protein Analysis

997

Amino Acids

109.01

Weight (kDa)

6.22

Isoelectric Point (pI)

37.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPDK_N	PF01326	143 - 179	7.5e-07	Pyruvate phosphate dikinase, AMP/ATP-binding domain
PPDK_N	PF01326	181 - 414	3.7e-23	Pyruvate phosphate dikinase, AMP/ATP-binding domain
PPDK_N	PF01326	425 - 480	1.9e-10	Pyruvate phosphate dikinase, AMP/ATP-binding domain
PEP-utilizers	PF00391	546 - 627	2.1e-22	PEP-utilising enzyme, mobile domain
PEP-utilizers_C	PF02896	642 - 996	4.1e-92	PEP-utilising enzyme, PEP-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004385)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G15530 AT4G15530 AT4G15530 AT4G15530 AT4G15530 AT4G15530 AT4G15530
fragaria_vesca	FvH4_4g16670
malus_domestica	MD13G1177400.v1.1 MD13G1177500.v1.1 MD16G1179300.v1.1 MD16G1179400.v1.1 MD16G1179500.v1.1
prunus_persica	Prupe.1G146600_v2.0.a1
pyrus_communis	pycom13g15300 pycom16g15050 pycom16g15060 pycom16g15070
rosa_chinensis	RchiOBHm_Chr4g0420131
rosa_laevigata	RLG00000007786
rosa_multiflora	Rmu_sc0001252.1_g000003 Rmu_sc0025222.1_g000001
rosa_roxburghii	Rroxscaffold_5G00362840
rosa_rugosa	Rorug04G0162400 Rorug04G0162500 Rorug04G0162600 Rorug04G0162600
rosa_samantha	Rh4AG223300 Rh4BG225700 Rh4CG236500 Rh4DG222100
rosa_wichuraiana	Rw4G019220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	2953
AccB1I	GGYRCC	1 cut(s)	2953
AccB7I	CCANNNNNTGG	2 cut(s)	1160, 2366
AccI	GTMKAC	1 cut(s)	1425
AccIII	TCCGGA	1 cut(s)	2541
AciI	CCGC	5 cut(s)	27, 1409, 1461, 1926, 2871
AclWI	GGATC	9 cut(s)	281, 962, 1514, 1527, 1712, 2157, 2512, 2765, 2796
AcoI	YGGCCR	2 cut(s)	229, 2820
AcsI	RAATTY	4 cut(s)	813, 956, 1302, 2187
AcuI	CTGAAG	5 cut(s)	65, 948, 1107, 2022, 2061
AcvI	CACGTG	1 cut(s)	2426
AcyI	GRCGYC	1 cut(s)	1969
AdeI	CACNNNGTG	2 cut(s)	73, 2530
AfaI	GTAC	8 cut(s)	358, 1384, 1396, 2399, 2578, 2708, 2784, 2955
AfeI	AGCGCT	2 cut(s)	755, 1457
AfiI	CCNNNNNNNGG	5 cut(s)	1160, 1227, 2200, 2366, 2501
AflIII	ACRYGT	3 cut(s)	789, 1486, 2064
AgeI	ACCGGT	1 cut(s)	1877
AgsI	TTSAA	8 cut(s)	181, 872, 941, 1091, 1451, 2183, 2593, 2765
AjnI	CCWGG	5 cut(s)	453, 490, 676, 1570, 2460
AjuI	GAANNNNNNNTTGG	6 cut(s)	416, 448, 1631, 1663, 2129, 2161
AloI	GAACNNNNNNTCC	4 cut(s)	200, 232, 1325, 1357
Alw21I	GWGCWC	2 cut(s)	2035, 2064
Alw26I	GTCTC	4 cut(s)	649, 1655, 1976, 2294
Alw44I	GTGCAC	1 cut(s)	2031
AlwI	GGATC	9 cut(s)	281, 962, 1514, 1527, 1712, 2157, 2512, 2765, 2796
AlwNI	CAGNNNCTG	3 cut(s)	896, 2303, 2518
Ama87I	CYCGRG	4 cut(s)	222, 352, 1024, 2329
Aor13HI	TCCGGA	1 cut(s)	2541
Aor51HI	AGCGCT	2 cut(s)	755, 1457
AoxI	GGCC	4 cut(s)	229, 1029, 2820, 2916
ApaLI	GTGCAC	1 cut(s)	2031
ApoI	RAATTY	4 cut(s)	813, 956, 1302, 2187
AsiGI	ACCGGT	1 cut(s)	1877
Asp700I	GAANNNNTTC	2 cut(s)	1120, 2561
Asp718I	GGTACC	1 cut(s)	2953
AspLEI	GCGC	2 cut(s)	756, 1458
AspS9I	GGNCC	2 cut(s)	349, 1029
AsuC2I	CCSGG	3 cut(s)	238, 1670, 2375
AsuNHI	GCTAGC	2 cut(s)	467, 2968
AvaI	CYCGRG	4 cut(s)	222, 352, 1024, 2329
AvaII	GGWCC	1 cut(s)	349
BaeGI	GKGCMC	1 cut(s)	2035
BalI	TGGCCA	1 cut(s)	2822
BamHI	GGATCC	1 cut(s)	1519
BanI	GGYRCC	1 cut(s)	2953
BanII	GRGCYC	1 cut(s)	325
BauI	CACGAG	1 cut(s)	1752
BbrPI	CACGTG	1 cut(s)	2426
BbsI	GAAGAC	3 cut(s)	605, 1646, 2201
Bbv12I	GWGCWC	2 cut(s)	2035, 2064
BbvCI	CCTCAGC	1 cut(s)	2913
BccI	CCATC	8 cut(s)	679, 1324, 1465, 1752, 2198, 2228, 2566, 2812
BceAI	ACGGC	1 cut(s)	217
BcgI	CGANNNNNNTGC	2 cut(s)	655, 689
BciT130I	CCWGG	5 cut(s)	455, 492, 678, 1572, 2462
BciVI	GTATCC	1 cut(s)	1240
BclI	TGATCA	1 cut(s)	2109
BcnI	CCSGG	3 cut(s)	238, 1670, 2375
BcoDI	GTCTC	4 cut(s)	649, 1655, 1976, 2294
BfmI	CTRYAG	6 cut(s)	305, 1077, 1422, 2295, 2380, 2442
BfoI	RGCGCY	2 cut(s)	757, 1459
BfuI	GTATCC	1 cut(s)	1240
BglI	GCCNNNNNGGC	1 cut(s)	493
BglII	AGATCT	1 cut(s)	2171
BlpI	GCTNAGC	1 cut(s)	2844
BmcAI	AGTACT	1 cut(s)	358
Bme1390I	CCNGG	8 cut(s)	238, 455, 492, 678, 1572, 1670, 2375, 2462
Bme18I	GGWCC	1 cut(s)	349
BmeT110I	CYCGRG	4 cut(s)	222, 352, 1024, 2329
BmgT120I	GGNCC	2 cut(s)	349, 1029
BmrFI	CCNGG	8 cut(s)	238, 455, 492, 678, 1572, 1670, 2375, 2462
BmrI	ACTGGG	3 cut(s)	65, 279, 2528
BmsI	GCATC	7 cut(s)	250, 480, 1056, 1574, 1703, 1772, 1996
BmtI	GCTAGC	2 cut(s)	471, 2972
BmuI	ACTGGG	3 cut(s)	65, 279, 2528
BpiI	GAAGAC	3 cut(s)	605, 1646, 2201
BplI	GAGNNNNNCTC	4 cut(s)	1842, 1874, 2651, 2683
BpmI	CTGGAG	1 cut(s)	1899
Bpu10I	CCTNAGC	3 cut(s)	1200, 2511, 2913
Bpu1102I	GCTNAGC	1 cut(s)	2844
BpuEI	CTTGAG	5 cut(s)	160, 1958, 2147, 2605, 2964
BpuMI	CCSGG	3 cut(s)	238, 1670, 2375
BsaAI	YACGTR	1 cut(s)	2426
BsaHI	GRCGYC	1 cut(s)	1969
BsaI	GGTCTC	1 cut(s)	1655
BsaJI	CCNNGG	5 cut(s)	491, 703, 1025, 2374, 2461
BsaWI	WCCGGW	6 cut(s)	151, 550, 1041, 1877, 2212, 2541
BsaXI	ACNNNNNCTCC	8 cut(s)	200, 230, 789, 819, 1197, 1227, 1566, 1596
Bsc4I	CCNNNNNNNGG	5 cut(s)	1160, 1227, 2200, 2366, 2501
Bse118I	RCCGGY	3 cut(s)	227, 1220, 1877
Bse3DI	GCAATG	2 cut(s)	1382, 2205
BseAI	TCCGGA	1 cut(s)	2541
BseBI	CCWGG	5 cut(s)	455, 492, 678, 1572, 2462
BseDI	CCNNGG	5 cut(s)	491, 703, 1025, 2374, 2461
BseGI	GGATG	7 cut(s)	116, 1374, 1763, 1787, 2209, 2220, 2323
BseLI	CCNNNNNNNGG	5 cut(s)	1160, 1227, 2200, 2366, 2501
BseMI	GCAATG	2 cut(s)	1382, 2205
BseMII	CTCAG	7 cut(s)	1214, 1523, 2304, 2472, 2525, 2559, 2904
BseRI	GAGGAG	3 cut(s)	581, 1902, 2811
BseSI	GKGCMC	1 cut(s)	2035
BseYI	CCCAGC	2 cut(s)	324, 1700
BsgI	GTGCAG	5 cut(s)	614, 681, 1623, 2448, 2859
Bsh1285I	CGRYCG	1 cut(s)	187
BshFI	GGCC	4 cut(s)	231, 1031, 2822, 2918
BshNI	GGYRCC	1 cut(s)	2953
BshTI	ACCGGT	1 cut(s)	1877
BsiEI	CGRYCG	1 cut(s)	187
BsiHKAI	GWGCWC	2 cut(s)	2035, 2064
BsiHKCI	CYCGRG	4 cut(s)	222, 352, 1024, 2329
BslFI	GGGAC	5 cut(s)	107, 1031, 1141, 1844, 2535
BslI	CCNNNNNNNGG	5 cut(s)	1160, 1227, 2200, 2366, 2501
BsmAI	GTCTC	4 cut(s)	649, 1655, 1976, 2294
BsmBI	CGTCTC	1 cut(s)	1976
BsmFI	GGGAC	5 cut(s)	107, 1031, 1141, 1844, 2535
BsmI	GAATGC	2 cut(s)	1692, 2757
BsnI	GGCC	4 cut(s)	231, 1031, 2822, 2918
Bso31I	GGTCTC	1 cut(s)	1655
BsoBI	CYCGRG	4 cut(s)	222, 352, 1024, 2329
Bsp1286I	GDGCHC	3 cut(s)	325, 2035, 2064
Bsp13I	TCCGGA	1 cut(s)	2541
Bsp1720I	GCTNAGC	1 cut(s)	2844
BspACI	CCGC	5 cut(s)	27, 1409, 1461, 1926, 2871
BspANI	GGCC	4 cut(s)	231, 1031, 2822, 2918
BspCNI	CTCAG	7 cut(s)	1213, 1522, 2305, 2473, 2524, 2560, 2905
BspEI	TCCGGA	1 cut(s)	2541
BspHI	TCATGA	1 cut(s)	2245
BspMAI	CTGCAG	5 cut(s)	309, 1081, 2299, 2384, 2446
BspOI	GCTAGC	2 cut(s)	471, 2972
BspPI	GGATC	9 cut(s)	281, 962, 1514, 1527, 1712, 2157, 2512, 2765, 2796
BspT107I	GGYRCC	1 cut(s)	2953
BspTNI	GGTCTC	1 cut(s)	1655
BsrDI	GCAATG	2 cut(s)	1382, 2205
BsrFI	RCCGGY	3 cut(s)	227, 1220, 1877
BssAI	RCCGGY	3 cut(s)	227, 1220, 1877
BssECI	CCNNGG	5 cut(s)	491, 703, 1025, 2374, 2461
BssNI	GRCGYC	1 cut(s)	1969
BssSI	CACGAG	1 cut(s)	1752
BssT1I	CCWWGG	1 cut(s)	703
Bst2BI	CACGAG	1 cut(s)	1752
Bst2UI	CCWGG	5 cut(s)	455, 492, 678, 1572, 2462
Bst4CI	ACNGT	8 cut(s)	106, 124, 212, 432, 694, 1298, 1348, 2711
BstACI	GRCGYC	1 cut(s)	1969
BstBAI	YACGTR	1 cut(s)	2426
BstDEI	CTNAG	9 cut(s)	291, 1200, 1509, 2313, 2481, 2511, 2568, 2844, 2913
BstEII	GGTNACC	3 cut(s)	620, 2215, 2264
BstF5I	GGATG	7 cut(s)	116, 1374, 1763, 1787, 2209, 2220, 2323
BstH2I	RGCGCY	2 cut(s)	757, 1459
BstHHI	GCGC	2 cut(s)	756, 1458
BstMAI	GTCTC	4 cut(s)	649, 1655, 1976, 2294
BstMCI	CGRYCG	1 cut(s)	187
BstNI	CCWGG	5 cut(s)	455, 492, 678, 1572, 2462
BstNSI	RCATGY	6 cut(s)	519, 793, 1267, 1490, 1694, 2068
BstPI	GGTNACC	3 cut(s)	620, 2215, 2264
BstSCI	CCNGG	8 cut(s)	236, 453, 490, 676, 1570, 1668, 2373, 2460
BstSFI	CTRYAG	6 cut(s)	305, 1077, 1422, 2295, 2380, 2442
BstSLI	GKGCMC	1 cut(s)	2035
BstV2I	GAAGAC	3 cut(s)	605, 1646, 2201
BstX2I	RGATCY	5 cut(s)	967, 1519, 1704, 2149, 2171
BstYI	RGATCY	5 cut(s)	967, 1519, 1704, 2149, 2171
BsuI	GTATCC	1 cut(s)	1240
BsuRI	GGCC	4 cut(s)	231, 1031, 2822, 2918
BtsCI	GGATG	7 cut(s)	116, 1374, 1763, 1787, 2209, 2220, 2323
BtsIMutI	CAGTG	9 cut(s)	78, 111, 129, 428, 690, 1158, 1344, 1551, 1606
CaiI	CAGNNNCTG	3 cut(s)	896, 2303, 2518
CciI	TCATGA	1 cut(s)	2245
CfoI	GCGC	2 cut(s)	756, 1458
Cfr10I	RCCGGY	3 cut(s)	227, 1220, 1877
Cfr13I	GGNCC	2 cut(s)	349, 1029
CseI	GACGC	2 cut(s)	1619, 1958
Csp6I	GTAC	8 cut(s)	357, 1383, 1395, 2398, 2577, 2707, 2783, 2954
CspAI	ACCGGT	1 cut(s)	1877
CviQI	GTAC	8 cut(s)	357, 1383, 1395, 2398, 2577, 2707, 2783, 2954
DdeI	CTNAG	9 cut(s)	291, 1200, 1509, 2313, 2481, 2511, 2568, 2844, 2913
DraI	TTTAAA	1 cut(s)	1068
DraIII	CACNNNGTG	2 cut(s)	73, 2530
EaeI	YGGCCR	2 cut(s)	229, 2820
Eco130I	CCWWGG	1 cut(s)	703
Eco24I	GRGCYC	1 cut(s)	325
Eco31I	GGTCTC	1 cut(s)	1655
Eco32I	GATATC	1 cut(s)	1792
Eco47I	GGWCC	1 cut(s)	349
Eco47III	AGCGCT	2 cut(s)	755, 1457
Eco57I	CTGAAG	5 cut(s)	65, 948, 1107, 2022, 2061
Eco72I	CACGTG	1 cut(s)	2426
Eco88I	CYCGRG	4 cut(s)	222, 352, 1024, 2329
Eco91I	GGTNACC	3 cut(s)	620, 2215, 2264
EcoO65I	GGTNACC	3 cut(s)	620, 2215, 2264
EcoRII	CCWGG	5 cut(s)	453, 490, 676, 1570, 2460
EcoRV	GATATC	1 cut(s)	1792
EcoT14I	CCWWGG	1 cut(s)	703
EcoT22I	ATGCAT	2 cut(s)	1692, 1696
EcoT38I	GRGCYC	1 cut(s)	325
ErhI	CCWWGG	1 cut(s)	703
Esp3I	CGTCTC	1 cut(s)	1976
FalI	AAGNNNNNCTT	6 cut(s)	1084, 1116, 2745, 2777, 2837, 2869
FaqI	GGGAC	5 cut(s)	107, 1031, 1141, 1844, 2535
FauNDI	CATATG	1 cut(s)	763
FbaI	TGATCA	1 cut(s)	2109
FblI	GTMKAC	1 cut(s)	1425
FokI	GGATG	7 cut(s)	103, 1381, 1770, 1794, 2207, 2216, 2330
FriOI	GRGCYC	1 cut(s)	325
GlaI	GCGC	2 cut(s)	755, 1457
GsaI	CCCAGC	2 cut(s)	328, 1704
GsuI	CTGGAG	1 cut(s)	1899
HaeII	RGCGCY	2 cut(s)	757, 1459
HaeIII	GGCC	4 cut(s)	231, 1031, 2822, 2918
HgaI	GACGC	2 cut(s)	1619, 1958
HhaI	GCGC	2 cut(s)	756, 1458
Hin1I	GRCGYC	1 cut(s)	1969
Hin6I	GCGC	2 cut(s)	754, 1456
HinP1I	GCGC	2 cut(s)	754, 1456
HincII	GTYRAC	2 cut(s)	1084, 1492
HindII	GTYRAC	2 cut(s)	1084, 1492
HindIII	AAGCTT	2 cut(s)	1272, 2851
HinfI	GANTC	3 cut(s)	767, 1616, 2614
HpaI	GTTAAC	1 cut(s)	1084
Hpy166II	GTNNAC	9 cut(s)	134, 208, 1084, 1344, 1426, 1492, 2033, 2326, 2356
Hpy8I	GTNNAC	9 cut(s)	134, 208, 1084, 1344, 1426, 1492, 2033, 2326, 2356
HpyCH4III	ACNGT	8 cut(s)	106, 124, 212, 432, 694, 1298, 1348, 2711
HpyCH4IV	ACGT	3 cut(s)	252, 778, 2425
HpyF3I	CTNAG	9 cut(s)	291, 1200, 1509, 2313, 2481, 2511, 2568, 2844, 2913
HpySE526I	ACGT	3 cut(s)	252, 778, 2425
Hsp92I	GRCGYC	1 cut(s)	1969
HspAI	GCGC	2 cut(s)	754, 1456
Kpn2I	TCCGGA	1 cut(s)	2541
KpnI	GGTACC	1 cut(s)	2957
KroI	GCCGGC	1 cut(s)	227
KroNI	GCCGGC	1 cut(s)	229
Ksp22I	TGATCA	1 cut(s)	2109
KspAI	GTTAAC	1 cut(s)	1084
LmnI	GCTCC	8 cut(s)	221, 320, 608, 751, 1044, 1282, 1463, 1574
LweI	GCATC	7 cut(s)	250, 480, 1056, 1574, 1703, 1772, 1996
MaeII	ACGT	3 cut(s)	252, 778, 2425
MaeIII	GTNAC	7 cut(s)	3, 336, 426, 620, 2215, 2264, 2530
MflI	RGATCY	5 cut(s)	967, 1519, 1704, 2149, 2171
MhlI	GDGCHC	3 cut(s)	325, 2035, 2064
MlsI	TGGCCA	1 cut(s)	2822
MluCI	AATT	9 cut(s)	545, 813, 956, 1009, 1258, 1302, 2088, 2187, 2342
MluNI	TGGCCA	1 cut(s)	2822
MlyI	GAGTC	1 cut(s)	761
MmeI	TCCRAC	6 cut(s)	386, 822, 1547, 2482, 2578, 2842
Mox20I	TGGCCA	1 cut(s)	2822
Mph1103I	ATGCAT	2 cut(s)	1692, 1696
MroI	TCCGGA	1 cut(s)	2541
MroNI	GCCGGC	1 cut(s)	227
MroXI	GAANNNNTTC	2 cut(s)	1120, 2561
MscI	TGGCCA	1 cut(s)	2822
MslI	CAYNNNNRTG	3 cut(s)	2414, 2693, 2816
Msp20I	TGGCCA	1 cut(s)	2822
MspA1I	CMGCKG	1 cut(s)	1742
MspR9I	CCNGG	8 cut(s)	238, 455, 492, 678, 1572, 1670, 2375, 2462
Mva1269I	GAATGC	2 cut(s)	1692, 2757
MvaI	CCWGG	5 cut(s)	455, 492, 678, 1572, 2462
NaeI	GCCGGC	1 cut(s)	229
NciI	CCSGG	3 cut(s)	238, 1670, 2375
NdeI	CATATG	1 cut(s)	763
NgoMIV	GCCGGC	1 cut(s)	227
NheI	GCTAGC	2 cut(s)	467, 2968
NmuCI	GTSAC	6 cut(s)	3, 336, 426, 620, 2264, 2530
NsiI	ATGCAT	2 cut(s)	1692, 1696
NspI	RCATGY	6 cut(s)	519, 793, 1267, 1490, 1694, 2068
PaeI	GCATGC	3 cut(s)	519, 1267, 1694
PaeR7I	CTCGAG	1 cut(s)	2329
PagI	TCATGA	1 cut(s)	2245
PciI	ACATGT	3 cut(s)	789, 1486, 2064
PcsI	WCGNNNNNNNCGW	1 cut(s)	480
PctI	GAATGC	2 cut(s)	1692, 2757
PdiI	GCCGGC	1 cut(s)	229
PdmI	GAANNNNTTC	2 cut(s)	1120, 2561
PfeI	GAWTC	2 cut(s)	1616, 2614
PflFI	GACNNNGTC	1 cut(s)	692
PflMI	CCANNNNNTGG	2 cut(s)	1160, 2366
PinAI	ACCGGT	1 cut(s)	1877
Ple19I	CGATCG	1 cut(s)	187
PleI	GAGTC	1 cut(s)	761
PmaCI	CACGTG	1 cut(s)	2426
PmlI	CACGTG	1 cut(s)	2426
PpsI	GAGTC	1 cut(s)	761
Ppu21I	YACGTR	1 cut(s)	2426
PscI	ACATGT	3 cut(s)	789, 1486, 2064
Psp6I	CCWGG	5 cut(s)	453, 490, 676, 1570, 2460
PspCI	CACGTG	1 cut(s)	2426
PspEI	GGTNACC	3 cut(s)	620, 2215, 2264
PspFI	CCCAGC	2 cut(s)	324, 1700
PspGI	CCWGG	5 cut(s)	453, 490, 676, 1570, 2460
PspPI	GGNCC	2 cut(s)	349, 1029
PstI	CTGCAG	5 cut(s)	309, 1081, 2299, 2384, 2446
PstNI	CAGNNNCTG	3 cut(s)	896, 2303, 2518
PsuI	RGATCY	5 cut(s)	967, 1519, 1704, 2149, 2171
PsyI	GACNNNGTC	1 cut(s)	692
PvuI	CGATCG	1 cut(s)	187
PvuII	CAGCTG	1 cut(s)	1742
RsaI	GTAC	8 cut(s)	358, 1384, 1396, 2399, 2578, 2708, 2784, 2955
RsaNI	GTAC	8 cut(s)	357, 1383, 1395, 2398, 2577, 2707, 2783, 2954
RseI	CAYNNNNRTG	3 cut(s)	2414, 2693, 2816
Sau96I	GGNCC	2 cut(s)	349, 1029
ScaI	AGTACT	1 cut(s)	358
SchI	GAGTC	1 cut(s)	761
ScrFI	CCNGG	8 cut(s)	238, 455, 492, 678, 1572, 1670, 2375, 2462
SduI	GDGCHC	3 cut(s)	325, 2035, 2064
SfaNI	GCATC	7 cut(s)	250, 480, 1056, 1574, 1703, 1772, 1996
SfcI	CTRYAG	6 cut(s)	305, 1077, 1422, 2295, 2380, 2442
Sfr274I	CTCGAG	1 cut(s)	2329
SgrAI	CRCCGGYG	1 cut(s)	1877
SinI	GGWCC	1 cut(s)	349
SlaI	CTCGAG	1 cut(s)	2329
SmiMI	CAYNNNNRTG	3 cut(s)	2414, 2693, 2816
SmlI	CTYRAG	6 cut(s)	139, 1973, 2126, 2329, 2584, 2979
SmoI	CTYRAG	6 cut(s)	139, 1973, 2126, 2329, 2584, 2979
SphI	GCATGC	3 cut(s)	519, 1267, 1694
Sse9I	AATT	9 cut(s)	545, 813, 956, 1009, 1258, 1302, 2088, 2187, 2342
SsiI	CCGC	5 cut(s)	27, 1409, 1461, 1926, 2871
StyD4I	CCNGG	8 cut(s)	236, 453, 490, 676, 1570, 1668, 2373, 2460
StyI	CCWWGG	1 cut(s)	703
TaaI	ACNGT	8 cut(s)	106, 124, 212, 432, 694, 1298, 1348, 2711
TaiI	ACGT	3 cut(s)	255, 781, 2428
TaqI	TCGA	1 cut(s)	2330
TasI	AATT	9 cut(s)	545, 813, 956, 1009, 1258, 1302, 2088, 2187, 2342
TatI	WGTACW	1 cut(s)	356
TfiI	GAWTC	2 cut(s)	1616, 2614
TscAI	CASTG	9 cut(s)	78, 111, 129, 435, 697, 1165, 1351, 1558, 1606
TseFI	GTSAC	6 cut(s)	3, 336, 426, 620, 2264, 2530
Tsp45I	GTSAC	6 cut(s)	3, 336, 426, 620, 2264, 2530
TspDTI	ATGAA	5 cut(s)	437, 1268, 1452, 2234, 2925
TspGWI	ACGGA	3 cut(s)	472, 1673, 2183
TspRI	CASTG	9 cut(s)	78, 111, 129, 435, 697, 1165, 1351, 1558, 1606
Tth111I	GACNNNGTC	1 cut(s)	692
Van91I	CCANNNNNTGG	2 cut(s)	1160, 2366
VneI	GTGCAC	1 cut(s)	2031
VpaK11BI	GGWCC	1 cut(s)	349
XapI	RAATTY	4 cut(s)	813, 956, 1302, 2187
XbaI	TCTAGA	1 cut(s)	1306
XceI	RCATGY	6 cut(s)	519, 793, 1267, 1490, 1694, 2068
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2699
XhoI	CTCGAG	1 cut(s)	2329
XmiI	GTMKAC	1 cut(s)	1425
XmnI	GAANNNNTTC	2 cut(s)	1120, 2561
ZrmI	AGTACT	1 cut(s)	358
Zsp2I	ATGCAT	2 cut(s)	1692, 1696

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.