Rmu_sc0001309.1_g000002

RRP12-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001309.1
OG0006794
Physical Location & Seq
Forward (+)
14082 .. 15210
1129 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 417 bp
atggcgatgcattgctcggtttctgtgttcttggcgaacttgaccttggtgccctccctgagagtcttgcccaactcgtacttcccgacccggtccctcatccttgacgacttggcatatgatttgggctcttgggaggagacgcaagtagagatccgcctgtctgaggtcgtgctagggtttttctgcaacctcccaattccgcccaacatggaagccattgaaatggaagagccgccgctaacccccaccagcaccaccgccttcgacgccgacgccgacgccgacgccgacatctgcaccccaatcctcacgcgcttcggcaactccacgcgcgaggaccatcagcacctctgcgccgtcatcggcggcacggcccagggtttcaaagaccagaacctccctccggtcacatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUC173

Protein Analysis

138

Amino Acids

15.11

Weight (kDa)

4.2

Isoelectric Point (pI)

48.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEAT_RRP12_N PF25772 91 - 136 1.9e-13 RRP12-like, N-terminal HEAT repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 49
AccII CGCG 3 cut(s) 316, 334, 336
AciI CCGC 6 cut(s) 157, 203, 236, 239, 261, 369
AclWI GGATC 1 cut(s) 148
AcyI GRCGYC 4 cut(s) 270, 276, 282, 288
AfaI GTAC 1 cut(s) 80
AfiI CCNNNNNNNGG 2 cut(s) 166, 406
AgsI TTSAA 2 cut(s) 224, 388
AjnI CCWGG 1 cut(s) 378
AjuI GAANNNNNNNTTGG 4 cut(s) 29, 61, 65, 97
Alw26I GTCTC 1 cut(s) 134
AlwI GGATC 1 cut(s) 148
AoxI GGCC 1 cut(s) 375
AspLEI GCGC 3 cut(s) 318, 336, 359
AspS9I GGNCC 3 cut(s) 93, 340, 376
AsuC2I CCSGG 1 cut(s) 91
AvaII GGWCC 2 cut(s) 93, 340
BaeGI GKGCMC 1 cut(s) 54
BanI GGYRCC 1 cut(s) 49
BanII GRGCYC 1 cut(s) 131
BccI CCATC 1 cut(s) 351
BceAI ACGGC 2 cut(s) 344, 390
BciT130I CCWGG 1 cut(s) 380
BcnI CCSGG 1 cut(s) 91
BcoDI GTCTC 1 cut(s) 134
BfaI CTAG 1 cut(s) 176
BisI GCNGC 3 cut(s) 236, 239, 370
BlsI GCNGC 3 cut(s) 237, 240, 371
Bme1390I CCNGG 2 cut(s) 91, 380
Bme18I GGWCC 2 cut(s) 93, 340
BmgT120I GGNCC 3 cut(s) 93, 340, 376
BmiI GGNNCC 2 cut(s) 51, 95
BmrFI CCNGG 2 cut(s) 91, 380
BpuMI CCSGG 1 cut(s) 91
BsaHI GRCGYC 4 cut(s) 270, 276, 282, 288
BsaJI CCNNGG 3 cut(s) 45, 378, 379
BsaWI WCCGGW 1 cut(s) 406
Bsc4I CCNNNNNNNGG 2 cut(s) 166, 406
Bse3DI GCAATG 1 cut(s) 10
BseBI CCWGG 1 cut(s) 380
BseDI CCNNGG 3 cut(s) 45, 378, 379
BseGI GGATG 1 cut(s) 99
BseLI CCNNNNNNNGG 2 cut(s) 166, 406
BseMI GCAATG 1 cut(s) 10
BseMII CTCAG 2 cut(s) 50, 156
BseRI GAGGAG 1 cut(s) 152
BseSI GKGCMC 1 cut(s) 54
BsgI GTGCAG 1 cut(s) 283
Bsh1236I CGCG 3 cut(s) 316, 334, 336
BshFI GGCC 1 cut(s) 377
BshNI GGYRCC 1 cut(s) 49
BsiSI CCGG 2 cut(s) 91, 407
BslFI GGGAC 1 cut(s) 79
BslI CCNNNNNNNGG 2 cut(s) 166, 406
BsmAI GTCTC 1 cut(s) 134
BsmBI CGTCTC 1 cut(s) 134
BsmFI GGGAC 1 cut(s) 79
BsnI GGCC 1 cut(s) 377
Bsp1286I GDGCHC 2 cut(s) 54, 131
Bsp143I GATC 1 cut(s) 153
BspACI CCGC 6 cut(s) 157, 203, 236, 239, 261, 369
BspANI GGCC 1 cut(s) 377
BspCNI CTCAG 2 cut(s) 51, 157
BspFNI CGCG 3 cut(s) 316, 334, 336
BspLI GGNNCC 2 cut(s) 51, 95
BspPI GGATC 1 cut(s) 148
BspQI GCTCTTC 1 cut(s) 225
BspT107I GGYRCC 1 cut(s) 49
BsrDI GCAATG 1 cut(s) 10
BssECI CCNNGG 3 cut(s) 45, 378, 379
BssMI GATC 1 cut(s) 153
BssNI GRCGYC 4 cut(s) 270, 276, 282, 288
BssT1I CCWWGG 1 cut(s) 45
Bst2UI CCWGG 1 cut(s) 380
Bst6I CTCTTC 1 cut(s) 225
BstACI GRCGYC 4 cut(s) 270, 276, 282, 288
BstDEI CTNAG 2 cut(s) 59, 165
BstENI CCTNNNNNAGG 1 cut(s) 164
BstF5I GGATG 1 cut(s) 99
BstFNI CGCG 3 cut(s) 316, 334, 336
BstHHI GCGC 3 cut(s) 318, 336, 359
BstKTI GATC 1 cut(s) 156
BstMAI GTCTC 1 cut(s) 134
BstMBI GATC 1 cut(s) 153
BstMWI GCNNNNNNNGC 1 cut(s) 269
BstNI CCWGG 1 cut(s) 380
BstSCI CCNGG 2 cut(s) 89, 378
BstSLI GKGCMC 1 cut(s) 54
BstUI CGCG 3 cut(s) 316, 334, 336
BstX2I RGATCY 1 cut(s) 153
BstXI CCANNNNNNTGG 1 cut(s) 226
BstYI RGATCY 1 cut(s) 153
BsuRI GGCC 1 cut(s) 377
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 1 cut(s) 99
CfoI GCGC 3 cut(s) 318, 336, 359
Cfr13I GGNCC 3 cut(s) 93, 340, 376
CseI GACGC 5 cut(s) 151, 278, 284, 290, 296
Csp6I GTAC 1 cut(s) 79
CviAII CATG 2 cut(s) 211, 414
CviJI RGCY 4 cut(s) 129, 218, 235, 377
CviKI_1 RGCY 4 cut(s) 129, 218, 235, 377
CviQI GTAC 1 cut(s) 79
DdeI CTNAG 2 cut(s) 59, 165
DpnI GATC 1 cut(s) 155
DpnII GATC 1 cut(s) 153
Eam1104I CTCTTC 1 cut(s) 225
EarI CTCTTC 1 cut(s) 225
EciI GGCGGA 2 cut(s) 146, 192
Eco130I CCWWGG 1 cut(s) 45
Eco24I GRGCYC 1 cut(s) 131
Eco47I GGWCC 2 cut(s) 93, 340
EcoNI CCTNNNNNAGG 1 cut(s) 164
EcoRII CCWGG 1 cut(s) 378
EcoT14I CCWWGG 1 cut(s) 45
EcoT22I ATGCAT 1 cut(s) 12
EcoT38I GRGCYC 1 cut(s) 131
ErhI CCWWGG 1 cut(s) 45
Esp3I CGTCTC 1 cut(s) 134
FaeI CATG 2 cut(s) 214, 417
FaiI YATR 4 cut(s) 118, 120, 212, 415
FaqI GGGAC 1 cut(s) 79
FatI CATG 2 cut(s) 210, 413
FauNDI CATATG 1 cut(s) 118
Fnu4HI GCNGC 3 cut(s) 236, 239, 370
FokI GGATG 1 cut(s) 86
FriOI GRGCYC 1 cut(s) 131
Fsp4HI GCNGC 3 cut(s) 236, 239, 370
FspBI CTAG 1 cut(s) 176
GlaI GCGC 3 cut(s) 317, 335, 358
GluI GCNGC 3 cut(s) 236, 239, 370
HaeIII GGCC 1 cut(s) 377
HapII CCGG 2 cut(s) 91, 407
HgaI GACGC 5 cut(s) 151, 278, 284, 290, 296
HhaI GCGC 3 cut(s) 318, 336, 359
Hin1I GRCGYC 4 cut(s) 270, 276, 282, 288
Hin1II CATG 2 cut(s) 214, 417
Hin6I GCGC 3 cut(s) 316, 334, 357
HinP1I GCGC 3 cut(s) 316, 334, 357
HinfI GANTC 1 cut(s) 63
HpaII CCGG 2 cut(s) 91, 407
Hpy188I TCNGA 1 cut(s) 166
Hpy188III TCNNGA 1 cut(s) 85
Hpy99I CGWCG 4 cut(s) 272, 278, 284, 290
HpyAV CCTTC 1 cut(s) 274
HpyCH4V TGCA 3 cut(s) 10, 189, 300
HpyF10VI GCNNNNNNNGC 1 cut(s) 269
HpyF3I CTNAG 2 cut(s) 59, 165
Hsp92I GRCGYC 4 cut(s) 270, 276, 282, 288
Hsp92II CATG 2 cut(s) 214, 417
HspAI GCGC 3 cut(s) 316, 334, 357
Kzo9I GATC 1 cut(s) 153
LguI GCTCTTC 1 cut(s) 225
LpnPI CCDG 7 cut(s) 71, 104, 173, 265, 365, 392, 407
MaeI CTAG 1 cut(s) 176
MaeIII GTNAC 1 cut(s) 409
MalI GATC 1 cut(s) 155
MboI GATC 1 cut(s) 153
MboII GAAGA 1 cut(s) 242
MflI RGATCY 1 cut(s) 153
MhlI GDGCHC 2 cut(s) 54, 131
MluCI AATT 1 cut(s) 198
MlyI GAGTC 1 cut(s) 72
Mph1103I ATGCAT 1 cut(s) 12
MslI CAYNNNNRTG 1 cut(s) 224
MspI CCGG 2 cut(s) 91, 407
MspR9I CCNGG 2 cut(s) 91, 380
MvaI CCWGG 1 cut(s) 380
MvnI CGCG 3 cut(s) 316, 334, 336
MwoI GCNNNNNNNGC 1 cut(s) 269
NciI CCSGG 1 cut(s) 91
NdeI CATATG 1 cut(s) 118
NdeII GATC 1 cut(s) 153
NlaIII CATG 2 cut(s) 214, 417
NlaIV GGNNCC 2 cut(s) 51, 95
NmuCI GTSAC 1 cut(s) 409
NsiI ATGCAT 1 cut(s) 12
PasI CCCWGGG 1 cut(s) 379
PciSI GCTCTTC 1 cut(s) 225
PcsI WCGNNNNNNNCGW 4 cut(s) 83, 276, 282, 288
PflFI GACNNNGTC 1 cut(s) 91
PkrI GCNGC 3 cut(s) 237, 240, 371
PleI GAGTC 1 cut(s) 71
PpsI GAGTC 1 cut(s) 71
Psp6I CCWGG 1 cut(s) 378
PspGI CCWGG 1 cut(s) 378
PspN4I GGNNCC 2 cut(s) 51, 95
PspPI GGNCC 3 cut(s) 93, 340, 376
PsuI RGATCY 1 cut(s) 153
PsyI GACNNNGTC 1 cut(s) 91
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
RseI CAYNNNNRTG 1 cut(s) 224
SapI GCTCTTC 1 cut(s) 225
SatI GCNGC 3 cut(s) 236, 239, 370
Sau3AI GATC 1 cut(s) 153
Sau96I GGNCC 3 cut(s) 93, 340, 376
SchI GAGTC 1 cut(s) 72
ScrFI CCNGG 2 cut(s) 91, 380
SduI GDGCHC 2 cut(s) 54, 131
SetI ASST 5 cut(s) 47, 171, 195, 354, 402
SinI GGWCC 2 cut(s) 93, 340
SmiMI CAYNNNNRTG 1 cut(s) 224
Sse9I AATT 1 cut(s) 198
SsiI CCGC 6 cut(s) 157, 203, 236, 239, 261, 369
SspMI CTAG 1 cut(s) 176
StyD4I CCNGG 2 cut(s) 89, 378
StyI CCWWGG 1 cut(s) 45
TaqI TCGA 1 cut(s) 267
TasI AATT 1 cut(s) 198
TauI GCSGC 3 cut(s) 238, 241, 372
TseFI GTSAC 1 cut(s) 409
Tsp45I GTSAC 1 cut(s) 409
Tth111I GACNNNGTC 1 cut(s) 91
VpaK11BI GGWCC 2 cut(s) 93, 340
XagI CCTNNNNNAGG 1 cut(s) 164
XspI CTAG 1 cut(s) 176
Zsp2I ATGCAT 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.