Rmu_sc0001405.1_g000008

Protein of unknown function (DUF1777)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001405.1
OG0010002
Physical Location & Seq
Forward (+)
33440 .. 34248
809 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 531 bp
atgctagagtcaagtcagaactgcatggttcataacagtaatttacatgccagaatcaagaggtcacgtacgccggagcgcagcagagcacgccacacacgctcccgcacgcgctcgccggacttgtaccactcacgctccctctcccgttcccgcactccagaagaccgctcccaccgccgccgccaccaccaccgcagcccctccccggagtcgcggaagcgtgcgcggcgcgaggcgacggtggaggaggagaaggaccggcagaaggtggtgtcggaattcgtggaggggattgcgaaggagcagcagaagcagagcagcggtggtggagagggcggtgcggacgaggacgaggttgagatgatgaagaagttggggattccggtagggtttgattccacgaaggggaagccggtgcagggggccgatgtgagtggtgttagggccgtcacgaagcgccagcctagacagtacatgaaccgccgcggcggatttaaccggccattgcctgcagagcgcaatcgctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DUF1777

Protein Analysis

176

Amino Acids

20.23

Weight (kDa)

11.17

Isoelectric Point (pI)

99.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNRNP27 PF08648 117 - 172 1.3e-22 U4/U6.U5 small nuclear ribonucleoproteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 171
AccII CGCG 5 cut(s) 112, 217, 229, 234, 489
AcoI YGGCCR 1 cut(s) 503
AcsI RAATTY 1 cut(s) 281
AfaI GTAC 3 cut(s) 70, 128, 476
AfiI CCNNNNNNNGG 4 cut(s) 208, 268, 408, 422
Alw21I GWGCWC 1 cut(s) 91
AoxI GGCC 3 cut(s) 426, 447, 503
ApeKI GCWGC 4 cut(s) 81, 198, 307, 321
ApoI RAATTY 1 cut(s) 281
AspLEI GCGC 6 cut(s) 81, 114, 229, 234, 462, 522
AspS9I GGNCC 3 cut(s) 259, 426, 447
AsuC2I CCSGG 1 cut(s) 209
AvaII GGWCC 1 cut(s) 259
BbsI GAAGAC 1 cut(s) 171
Bbv12I GWGCWC 1 cut(s) 91
BbvI GCAGC 4 cut(s) 93, 210, 319, 333
BceAI ACGGC 1 cut(s) 434
BcnI CCSGG 1 cut(s) 209
BfaI CTAG 3 cut(s) 5, 468, 529
BfmI CTRYAG 1 cut(s) 513
BfoI RGCGCY 1 cut(s) 463
BisI GCNGC 9 cut(s) 82, 181, 184, 199, 230, 308, 322, 487, 490
BlsI GCNGC 9 cut(s) 83, 182, 185, 200, 231, 309, 323, 488, 491
Bme1390I CCNGG 1 cut(s) 209
Bme18I GGWCC 1 cut(s) 259
BmgT120I GGNCC 3 cut(s) 259, 426, 447
BmiI GGNNCC 1 cut(s) 427
BmrFI CCNGG 1 cut(s) 209
BpiI GAAGAC 1 cut(s) 171
BpmI CTGGAG 1 cut(s) 144
BpuMI CCSGG 1 cut(s) 209
BsaAI YACGTR 1 cut(s) 68
BsaJI CCNNGG 2 cut(s) 207, 487
BsaWI WCCGGW 1 cut(s) 385
BsaXI ACNNNNNCTCC 4 cut(s) 86, 116, 122, 152
Bsc4I CCNNNNNNNGG 4 cut(s) 208, 268, 408, 422
Bse118I RCCGGY 3 cut(s) 261, 415, 501
Bse3DI GCAATG 1 cut(s) 506
BseDI CCNNGG 2 cut(s) 207, 487
BseLI CCNNNNNNNGG 4 cut(s) 208, 268, 408, 422
BseMI GCAATG 1 cut(s) 506
BseRI GAGGAG 2 cut(s) 263, 266
BseXI GCAGC 4 cut(s) 93, 210, 319, 333
BsgI GTGCAG 1 cut(s) 440
Bsh1236I CGCG 5 cut(s) 112, 217, 229, 234, 489
BshFI GGCC 3 cut(s) 428, 449, 505
BsiHKAI GWGCWC 1 cut(s) 91
BsiSI CCGG 7 cut(s) 74, 119, 209, 262, 386, 416, 502
BsiWI CGTACG 1 cut(s) 68
BslI CCNNNNNNNGG 4 cut(s) 208, 268, 408, 422
BsnI GGCC 3 cut(s) 428, 449, 505
Bsp1286I GDGCHC 1 cut(s) 91
BspANI GGCC 3 cut(s) 428, 449, 505
BspFNI CGCG 5 cut(s) 112, 217, 229, 234, 489
BspLI GGNNCC 1 cut(s) 427
BspMAI CTGCAG 1 cut(s) 517
BsrBI CCGCTC 1 cut(s) 171
BsrDI GCAATG 1 cut(s) 506
BsrFI RCCGGY 3 cut(s) 261, 415, 501
BssAI RCCGGY 3 cut(s) 261, 415, 501
BssECI CCNNGG 2 cut(s) 207, 487
Bst4CI ACNGT 3 cut(s) 38, 244, 474
BstBAI YACGTR 1 cut(s) 68
BstC8I GCNNGC 6 cut(s) 91, 110, 116, 225, 464, 513
BstDSI CCRYGG 1 cut(s) 487
BstFNI CGCG 5 cut(s) 112, 217, 229, 234, 489
BstH2I RGCGCY 1 cut(s) 463
BstHHI GCGC 6 cut(s) 81, 114, 229, 234, 462, 522
BstMWI GCNNNNNNNGC 6 cut(s) 90, 99, 177, 229, 313, 517
BstNSI RCATGY 1 cut(s) 50
BstSCI CCNGG 1 cut(s) 207
BstSFI CTRYAG 1 cut(s) 513
BstUI CGCG 5 cut(s) 112, 217, 229, 234, 489
BstV1I GCAGC 4 cut(s) 93, 210, 319, 333
BstV2I GAAGAC 1 cut(s) 171
BsuRI GGCC 3 cut(s) 428, 449, 505
BtgI CCRYGG 1 cut(s) 487
Cac8I GCNNGC 6 cut(s) 91, 110, 116, 225, 464, 513
CfoI GCGC 6 cut(s) 81, 114, 229, 234, 462, 522
Cfr10I RCCGGY 3 cut(s) 261, 415, 501
Cfr13I GGNCC 3 cut(s) 259, 426, 447
Cfr42I CCGCGG 1 cut(s) 490
Csp6I GTAC 3 cut(s) 69, 127, 475
CviAII CATG 3 cut(s) 25, 47, 478
CviJI RGCY 6 cut(s) 201, 415, 428, 449, 466, 505
CviKI_1 RGCY 6 cut(s) 201, 415, 428, 449, 466, 505
CviQI GTAC 3 cut(s) 69, 127, 475
EaeI YGGCCR 1 cut(s) 503
EciI GGCGGA 1 cut(s) 507
Eco47I GGWCC 1 cut(s) 259
EcoRI GAATTC 1 cut(s) 281
FaeI CATG 3 cut(s) 28, 50, 481
FaiI YATR 4 cut(s) 26, 33, 48, 479
FatI CATG 3 cut(s) 24, 46, 477
FauI CCCGC 2 cut(s) 113, 161
Fnu4HI GCNGC 9 cut(s) 82, 181, 184, 199, 230, 308, 322, 487, 490
Fsp4HI GCNGC 9 cut(s) 82, 181, 184, 199, 230, 308, 322, 487, 490
FspBI CTAG 3 cut(s) 5, 468, 529
GlaI GCGC 6 cut(s) 80, 113, 228, 233, 461, 521
GluI GCNGC 9 cut(s) 82, 181, 184, 199, 230, 308, 322, 487, 490
GsuI CTGGAG 1 cut(s) 144
HaeII RGCGCY 1 cut(s) 463
HaeIII GGCC 3 cut(s) 428, 449, 505
HapII CCGG 7 cut(s) 74, 119, 209, 262, 386, 416, 502
HhaI GCGC 6 cut(s) 81, 114, 229, 234, 462, 522
Hin1II CATG 3 cut(s) 28, 50, 481
Hin6I GCGC 6 cut(s) 79, 112, 227, 232, 460, 520
HinP1I GCGC 6 cut(s) 79, 112, 227, 232, 460, 520
HinfI GANTC 5 cut(s) 8, 54, 212, 382, 398
HpaII CCGG 7 cut(s) 74, 119, 209, 262, 386, 416, 502
Hpy188I TCNGA 2 cut(s) 18, 280
Hpy188III TCNNGA 3 cut(s) 58, 161, 454
Hpy99I CGWCG 1 cut(s) 244
HpyAV CCTTC 4 cut(s) 250, 262, 295, 400
HpyCH4III ACNGT 3 cut(s) 38, 244, 474
HpyCH4IV ACGT 1 cut(s) 67
HpyCH4V TGCA 3 cut(s) 24, 421, 515
HpyF10VI GCNNNNNNNGC 6 cut(s) 90, 99, 177, 229, 313, 517
HpySE526I ACGT 1 cut(s) 67
Hsp92II CATG 3 cut(s) 28, 50, 481
HspAI GCGC 6 cut(s) 79, 112, 227, 232, 460, 520
KspI CCGCGG 1 cut(s) 490
LmnI GCTCC 5 cut(s) 76, 107, 143, 176, 304
Lsp1109I GCAGC 4 cut(s) 93, 210, 319, 333
MaeI CTAG 3 cut(s) 5, 468, 529
MaeII ACGT 1 cut(s) 67
MaeIII GTNAC 2 cut(s) 63, 451
MbiI CCGCTC 1 cut(s) 171
MboII GAAGA 2 cut(s) 176, 382
MhlI GDGCHC 1 cut(s) 91
MluCI AATT 2 cut(s) 40, 281
MlyI GAGTC 2 cut(s) 17, 221
MmeI TCCRAC 1 cut(s) 258
MseI TTAA 1 cut(s) 498
MspA1I CMGCKG 2 cut(s) 324, 489
MspI CCGG 7 cut(s) 74, 119, 209, 262, 386, 416, 502
MspR9I CCNGG 1 cut(s) 209
MteI GCGCNGCGC 1 cut(s) 230
MvnI CGCG 5 cut(s) 112, 217, 229, 234, 489
MwoI GCNNNNNNNGC 6 cut(s) 90, 99, 177, 229, 313, 517
NciI CCSGG 1 cut(s) 209
NlaIII CATG 3 cut(s) 28, 50, 481
NlaIV GGNNCC 1 cut(s) 427
NmuCI GTSAC 2 cut(s) 63, 451
NspI RCATGY 1 cut(s) 50
PfeI GAWTC 3 cut(s) 54, 382, 398
Pfl23II CGTACG 1 cut(s) 68
PkrI GCNGC 9 cut(s) 83, 182, 185, 200, 231, 309, 323, 488, 491
PleI GAGTC 2 cut(s) 16, 220
PpsI GAGTC 2 cut(s) 16, 220
Ppu21I YACGTR 1 cut(s) 68
PspLI CGTACG 1 cut(s) 68
PspN4I GGNNCC 1 cut(s) 427
PspPI GGNCC 3 cut(s) 259, 426, 447
PstI CTGCAG 1 cut(s) 517
RsaI GTAC 3 cut(s) 70, 128, 476
RsaNI GTAC 3 cut(s) 69, 127, 475
SacII CCGCGG 1 cut(s) 490
SaqAI TTAA 1 cut(s) 498
SatI GCNGC 9 cut(s) 82, 181, 184, 199, 230, 308, 322, 487, 490
Sau96I GGNCC 3 cut(s) 259, 426, 447
SchI GAGTC 2 cut(s) 17, 221
ScrFI CCNGG 1 cut(s) 209
SduI GDGCHC 1 cut(s) 91
SetI ASST 4 cut(s) 65, 70, 273, 360
SfcI CTRYAG 1 cut(s) 513
Sfr303I CCGCGG 1 cut(s) 490
SgrBI CCGCGG 1 cut(s) 490
SinI GGWCC 1 cut(s) 259
Sse9I AATT 2 cut(s) 40, 281
SspMI CTAG 3 cut(s) 5, 468, 529
StyD4I CCNGG 1 cut(s) 207
TaaI ACNGT 3 cut(s) 38, 244, 474
TaiI ACGT 1 cut(s) 70
TasI AATT 2 cut(s) 40, 281
TatI WGTACW 1 cut(s) 474
TauI GCSGC 5 cut(s) 183, 186, 232, 489, 492
TfiI GAWTC 3 cut(s) 54, 382, 398
Tru1I TTAA 1 cut(s) 498
Tru9I TTAA 1 cut(s) 498
TseFI GTSAC 2 cut(s) 63, 451
TseI GCWGC 4 cut(s) 81, 198, 307, 321
Tsp45I GTSAC 2 cut(s) 63, 451
TspDTI ATGAA 3 cut(s) 20, 383, 494
VpaK11BI GGWCC 1 cut(s) 259
XapI RAATTY 1 cut(s) 281
XceI RCATGY 1 cut(s) 50
XspI CTAG 3 cut(s) 5, 468, 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.