Rmu_sc0001551.1_g000031

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001551.1
OG0011578
Physical Location & Seq
Reverse (-)
134203 .. 135232
1030 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 831 bp
atgaattcagttgatcgaattggtttgcagaactcgtttcagctggcattcttgacctggacatggtttgaatttggccagaattcatgctttaatcgaagaacaaaagatatcgtcattaagagtagtatgggactgggtgtgcagttgatatgtggttatgtgaccctccctctttacgcactggttacccagatggggtctggcatgaggaaagcagtatttactgagctcgtagttgatggcctcaaaaactggcacaagaatgcaagacgcagactatcgaagaatggatcctctacttcaaggaaatcatcaaattctgcacacatttattcaaccgatgactcctcatttacagacaatgaatgtagctacaaaactcctaatcccaccatagtgagaagctcttcttctacctccgaaataattgaagagcgcgttccagccggtttaagtccgaagaatggatccactacttcaaggaaatcctccgattctgcacatttttattcaaatgataactcctcatttccagacaatgaatgtagcaacaaaaatcctaatcccaccatagtgagaagctcttcttctacctccgaaataattgaagagcaagttccacccggtttaattccgaacccggatactacaagtttaccaactccagaaatcaccagagaggaacagaatcccaacatcatcaacaaaataatctatgacggagagatttcttttgggagcagttggaaattaaagctggagagcatgagcaagagaagtggggaaatcacttcaataattgaagacgaacttaacttcgatcggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

276

Amino Acids

30.67

Weight (kDa)

6.35

Isoelectric Point (pI)

61.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 8 - 114 7.2e-36 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 441
AclWI GGATC 4 cut(s) 288, 301, 465, 478
AcoI YGGCCR 1 cut(s) 76
AcsI RAATTY 4 cut(s) 4, 71, 82, 319
AfiI CCNNNNNNNGG 3 cut(s) 63, 198, 467
AgsI TTSAA 9 cut(s) 71, 306, 339, 434, 483, 516, 611, 798, 806
AjnI CCWGG 1 cut(s) 56
AleI CACNNNNGTG 2 cut(s) 398, 575
AluBI AGCT 6 cut(s) 43, 232, 375, 408, 585, 760
AluI AGCT 6 cut(s) 43, 232, 375, 408, 585, 760
Alw21I GWGCWC 1 cut(s) 234
AlwI GGATC 4 cut(s) 288, 301, 465, 478
AoxI GGCC 2 cut(s) 76, 244
ApoI RAATTY 4 cut(s) 4, 71, 82, 319
Asp700I GAANNNNTTC 2 cut(s) 409, 586
AspLEI GCGC 1 cut(s) 441
AsuC2I CCSGG 2 cut(s) 627, 644
AsuHPI GGTGA 1 cut(s) 667
BalI TGGCCA 1 cut(s) 78
BamHI GGATCC 2 cut(s) 293, 470
BanII GRGCYC 1 cut(s) 234
BbsI GAAGAC 1 cut(s) 813
Bbv12I GWGCWC 1 cut(s) 234
BccI CCATC 2 cut(s) 190, 236
BciT130I CCWGG 1 cut(s) 58
BciVI GTATCC 1 cut(s) 640
BcnI CCSGG 2 cut(s) 627, 644
BfuI GTATCC 1 cut(s) 640
Bme1390I CCNGG 3 cut(s) 58, 627, 644
BmiI GGNNCC 2 cut(s) 295, 472
BmrFI CCNGG 3 cut(s) 58, 627, 644
BmrI ACTGGG 1 cut(s) 146
BmuI ACTGGG 1 cut(s) 146
BpiI GAAGAC 1 cut(s) 813
BpmI CTGGAG 2 cut(s) 651, 782
BpuMI CCSGG 2 cut(s) 627, 644
Bsc4I CCNNNNNNNGG 3 cut(s) 63, 198, 467
Bse118I RCCGGY 1 cut(s) 449
Bse1I ACTGG 3 cut(s) 141, 189, 260
BseBI CCWGG 1 cut(s) 58
BseLI CCNNNNNNNGG 3 cut(s) 63, 198, 467
BseMII CTCAG 1 cut(s) 219
BseNI ACTGG 3 cut(s) 141, 189, 260
BseRI GAGGAG 2 cut(s) 340, 517
BsgI GTGCAG 3 cut(s) 164, 309, 486
Bsh1236I CGCG 1 cut(s) 441
Bsh1285I CGRYCG 1 cut(s) 826
BshFI GGCC 2 cut(s) 78, 246
BsiEI CGRYCG 1 cut(s) 826
BsiHKAI GWGCWC 1 cut(s) 234
BsiSI CCGG 3 cut(s) 450, 627, 644
BslFI GGGAC 1 cut(s) 147
BslI CCNNNNNNNGG 3 cut(s) 63, 198, 467
BsmFI GGGAC 1 cut(s) 147
BsmI GAATGC 2 cut(s) 47, 271
BsnI GGCC 2 cut(s) 78, 246
Bsp1286I GDGCHC 1 cut(s) 234
Bsp143I GATC 4 cut(s) 13, 293, 470, 823
BspANI GGCC 2 cut(s) 78, 246
BspCNI CTCAG 1 cut(s) 220
BspFNI CGCG 1 cut(s) 441
BspLI GGNNCC 2 cut(s) 295, 472
BspPI GGATC 4 cut(s) 288, 301, 465, 478
BspQI GCTCTTC 4 cut(s) 415, 429, 592, 606
BsrFI RCCGGY 1 cut(s) 449
BsrI ACTGG 3 cut(s) 141, 189, 260
BssAI RCCGGY 1 cut(s) 449
BssMI GATC 4 cut(s) 13, 293, 470, 823
Bst2UI CCWGG 1 cut(s) 58
Bst6I CTCTTC 4 cut(s) 415, 429, 592, 606
BstC8I GCNNGC 1 cut(s) 45
BstDEI CTNAG 1 cut(s) 228
BstEII GGTNACC 1 cut(s) 187
BstFNI CGCG 1 cut(s) 441
BstHHI GCGC 1 cut(s) 441
BstKTI GATC 4 cut(s) 16, 296, 473, 826
BstMBI GATC 4 cut(s) 13, 293, 470, 823
BstMCI CGRYCG 1 cut(s) 826
BstNI CCWGG 1 cut(s) 58
BstPI GGTNACC 1 cut(s) 187
BstSCI CCNGG 3 cut(s) 56, 625, 642
BstUI CGCG 1 cut(s) 441
BstV2I GAAGAC 1 cut(s) 813
BstX2I RGATCY 2 cut(s) 293, 470
BstYI RGATCY 2 cut(s) 293, 470
BsuI GTATCC 1 cut(s) 640
BsuRI GGCC 2 cut(s) 78, 246
BtsIMutI CAGTG 1 cut(s) 182
Cac8I GCNNGC 1 cut(s) 45
CfoI GCGC 1 cut(s) 441
Cfr10I RCCGGY 1 cut(s) 449
CseI GACGC 1 cut(s) 282
CspCI CAANNNNNGTGG 2 cut(s) 763, 798
CviAII CATG 4 cut(s) 63, 87, 208, 769
CviJI RGCY 9 cut(s) 43, 78, 232, 246, 375, 408, 449, 585, 760
CviKI_1 RGCY 9 cut(s) 43, 78, 232, 246, 375, 408, 449, 585, 760
DdeI CTNAG 1 cut(s) 228
DpnI GATC 4 cut(s) 15, 295, 472, 825
DpnII GATC 4 cut(s) 13, 293, 470, 823
EaeI YGGCCR 1 cut(s) 76
Eam1104I CTCTTC 4 cut(s) 415, 429, 592, 606
EarI CTCTTC 4 cut(s) 415, 429, 592, 606
Ecl136II GAGCTC 1 cut(s) 232
Eco24I GRGCYC 1 cut(s) 234
Eco32I GATATC 1 cut(s) 112
Eco53kI GAGCTC 1 cut(s) 232
Eco91I GGTNACC 1 cut(s) 187
EcoICRI GAGCTC 1 cut(s) 232
EcoO65I GGTNACC 1 cut(s) 187
EcoRI GAATTC 2 cut(s) 4, 82
EcoRII CCWGG 1 cut(s) 56
EcoRV GATATC 1 cut(s) 112
EcoT38I GRGCYC 1 cut(s) 234
FaeI CATG 4 cut(s) 66, 90, 211, 772
FalI AAGNNNNNCTT 6 cut(s) 397, 429, 574, 606, 798, 830
FaqI GGGAC 1 cut(s) 147
FatI CATG 4 cut(s) 62, 86, 207, 768
FriOI GRGCYC 1 cut(s) 234
GlaI GCGC 1 cut(s) 440
GsuI CTGGAG 2 cut(s) 651, 782
HaeIII GGCC 2 cut(s) 78, 246
HapII CCGG 3 cut(s) 450, 627, 644
HgaI GACGC 1 cut(s) 282
HhaI GCGC 1 cut(s) 441
Hin1II CATG 4 cut(s) 66, 90, 211, 772
Hin6I GCGC 1 cut(s) 439
HinP1I GCGC 1 cut(s) 439
HinfI GANTC 3 cut(s) 347, 497, 691
HpaII CCGG 3 cut(s) 450, 627, 644
HphI GGTGA 1 cut(s) 667
Hpy166II GTNNAC 1 cut(s) 659
Hpy188I TCNGA 5 cut(s) 424, 462, 496, 601, 639
Hpy188III TCNNGA 3 cut(s) 52, 536, 668
Hpy8I GTNNAC 1 cut(s) 659
HpyCH4V TGCA 5 cut(s) 28, 145, 269, 326, 503
HpyF3I CTNAG 1 cut(s) 228
Hsp92II CATG 4 cut(s) 66, 90, 211, 772
HspAI GCGC 1 cut(s) 439
Kzo9I GATC 4 cut(s) 13, 293, 470, 823
LguI GCTCTTC 4 cut(s) 415, 429, 592, 606
LmnI GCTCC 1 cut(s) 741
MaeIII GTNAC 2 cut(s) 163, 187
MalI GATC 4 cut(s) 15, 295, 472, 825
MboI GATC 4 cut(s) 13, 293, 470, 823
MflI RGATCY 2 cut(s) 293, 470
MhlI GDGCHC 1 cut(s) 234
MlsI TGGCCA 1 cut(s) 78
MluNI TGGCCA 1 cut(s) 78
MlyI GAGTC 1 cut(s) 341
MmeI TCCRAC 1 cut(s) 728
Mox20I TGGCCA 1 cut(s) 78
MroXI GAANNNNTTC 2 cut(s) 409, 586
MscI TGGCCA 1 cut(s) 78
MseI TTAA 6 cut(s) 93, 120, 455, 632, 755, 816
MslI CAYNNNNRTG 3 cut(s) 264, 398, 575
Msp20I TGGCCA 1 cut(s) 78
MspA1I CMGCKG 1 cut(s) 43
MspI CCGG 3 cut(s) 450, 627, 644
MspR9I CCNGG 3 cut(s) 58, 627, 644
Mva1269I GAATGC 2 cut(s) 47, 271
MvaI CCWGG 1 cut(s) 58
MvnI CGCG 1 cut(s) 441
NciI CCSGG 2 cut(s) 627, 644
NdeII GATC 4 cut(s) 13, 293, 470, 823
NlaIII CATG 4 cut(s) 66, 90, 211, 772
NlaIV GGNNCC 2 cut(s) 295, 472
NmuCI GTSAC 1 cut(s) 163
OliI CACNNNNGTG 2 cut(s) 398, 575
PciSI GCTCTTC 4 cut(s) 415, 429, 592, 606
PctI GAATGC 2 cut(s) 47, 271
PdmI GAANNNNTTC 2 cut(s) 409, 586
PfeI GAWTC 2 cut(s) 497, 691
Ple19I CGATCG 1 cut(s) 826
PleI GAGTC 1 cut(s) 341
PpsI GAGTC 1 cut(s) 341
Psp124BI GAGCTC 1 cut(s) 234
Psp6I CCWGG 1 cut(s) 56
PspEI GGTNACC 1 cut(s) 187
PspGI CCWGG 1 cut(s) 56
PspN4I GGNNCC 2 cut(s) 295, 472
PsuI RGATCY 2 cut(s) 293, 470
PvuI CGATCG 1 cut(s) 826
PvuII CAGCTG 1 cut(s) 43
RseI CAYNNNNRTG 3 cut(s) 264, 398, 575
SacI GAGCTC 1 cut(s) 234
SapI GCTCTTC 4 cut(s) 415, 429, 592, 606
SaqAI TTAA 6 cut(s) 93, 120, 455, 632, 755, 816
Sau3AI GATC 4 cut(s) 13, 293, 470, 823
SchI GAGTC 1 cut(s) 341
ScrFI CCNGG 3 cut(s) 58, 627, 644
SduI GDGCHC 1 cut(s) 234
SetI ASST 9 cut(s) 45, 59, 234, 377, 410, 422, 587, 599, 762
SmiMI CAYNNNNRTG 3 cut(s) 264, 398, 575
SstI GAGCTC 1 cut(s) 234
StyD4I CCNGG 3 cut(s) 56, 625, 642
TaqI TCGA 4 cut(s) 16, 97, 284, 822
TfiI GAWTC 2 cut(s) 497, 691
Tru1I TTAA 6 cut(s) 93, 120, 455, 632, 755, 816
Tru9I TTAA 6 cut(s) 93, 120, 455, 632, 755, 816
TscAI CASTG 1 cut(s) 189
TseFI GTSAC 1 cut(s) 163
Tsp45I GTSAC 1 cut(s) 163
TspDTI ATGAA 4 cut(s) 17, 75, 381, 558
TspGWI ACGGA 1 cut(s) 738
TspRI CASTG 1 cut(s) 189
XapI RAATTY 4 cut(s) 4, 71, 82, 319
XcmI CCANNNNNNNNNTGG 1 cut(s) 200
XmnI GAANNNNTTC 2 cut(s) 409, 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.