Rmu_sc0002129.1_g000049

GPI-anchored protein At3g06035-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002129.1
OG0008438
Physical Location & Seq
Reverse (-)
196216 .. 197285
1070 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 708 bp
atgctggcggtttggttcaatggctttgacgagtttgctcgaaaaagcaatggtgcagtgtcccctgaacttgaggaactcccttctttgggcaaagtctatacgagaatgcttgttgaaaagacaagtggaatagggcaaccactactagttgatgaggaggataatcttcttcaaggcatcaacagctatagggcttctgccaacttaacagcccttacaaagaatgacaatgccgagtgcctggcagatgagatagctgaccaattcaaaaagcaaccctgtacaaacaccacaggttccttcacggtaccaggcactgaaacccagtttgccaactttccagaccttttagccaaatgtcatttgaatgtctcaaacacaagggatggagtcataatgccttcttgcgttccgaatctggatcctagtctcgtccttgccaacttcactaagtctcagtactcgtcgaatcttaatcagacaaattttacaggaattggaattggttctgaagataactggattgttgttattttgaccacgagcacacccgaaggaagctatgttcctgcacaaagcactgacagtgccactggcaaccaagaggtttatcataccaattttctgcttttccgacctagagaggacaatgattctcccattggagctgcttccatggtggaattcgatttcgtacttgcataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

235

Amino Acids

25.57

Weight (kDa)

4.36

Isoelectric Point (pI)

32.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g19230 PF25884 54 - 180 2.8e-60 Uncharacterized GPI-anchored protein At5g19230-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 310
AccB1I GGYRCC 1 cut(s) 310
AciI CCGC 1 cut(s) 8
AclWI GGATC 2 cut(s) 419, 432
AcsI RAATTY 2 cut(s) 487, 686
AcuI CTGAAG 1 cut(s) 534
AfaI GTAC 4 cut(s) 286, 312, 464, 699
AfiI CCNNNNNNNGG 2 cut(s) 88, 89
AgsI TTSAA 5 cut(s) 19, 119, 176, 271, 370
AhlI ACTAGT 1 cut(s) 148
AjnI CCWGG 2 cut(s) 243, 313
AloI GAACNNNNNNTCC 2 cut(s) 552, 584
AluBI AGCT 4 cut(s) 189, 260, 564, 671
AluI AGCT 4 cut(s) 189, 260, 564, 671
Alw21I GWGCWC 1 cut(s) 551
Alw26I GTCTC 3 cut(s) 379, 437, 462
AlwI GGATC 2 cut(s) 419, 432
AlwNI CAGNNNCTG 1 cut(s) 320
ApeKI GCWGC 1 cut(s) 671
ApoI RAATTY 2 cut(s) 487, 686
Asp700I GAANNNNTTC 1 cut(s) 508
Asp718I GGTACC 1 cut(s) 310
BamHI GGATCC 1 cut(s) 424
BanI GGYRCC 1 cut(s) 310
BauI CACGAG 1 cut(s) 544
Bbv12I GWGCWC 1 cut(s) 551
BbvI GCAGC 1 cut(s) 658
BccI CCATC 1 cut(s) 383
BciT130I CCWGG 2 cut(s) 245, 315
BcoDI GTCTC 3 cut(s) 379, 437, 462
BcuI ACTAGT 1 cut(s) 148
BfaI CTAG 3 cut(s) 149, 429, 642
BfmI CTRYAG 1 cut(s) 190
BisI GCNGC 1 cut(s) 672
BlsI GCNGC 1 cut(s) 673
BmcAI AGTACT 1 cut(s) 464
Bme1390I CCNGG 2 cut(s) 245, 315
BmiI GGNNCC 3 cut(s) 301, 312, 426
BmrFI CCNGG 2 cut(s) 245, 315
BmrI ACTGGG 1 cut(s) 322
BmsI GCATC 1 cut(s) 189
BmuI ACTGGG 1 cut(s) 322
BpuEI CTTGAG 1 cut(s) 92
BsaJI CCNNGG 1 cut(s) 678
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 89
Bse1I ACTGG 3 cut(s) 328, 527, 601
Bse3DI GCAATG 1 cut(s) 55
BseBI CCWGG 2 cut(s) 245, 315
BseDI CCNNGG 1 cut(s) 678
BseGI GGATG 1 cut(s) 394
BseLI CCNNNNNNNGG 2 cut(s) 88, 89
BseMI GCAATG 1 cut(s) 55
BseMII CTCAG 1 cut(s) 473
BseNI ACTGG 3 cut(s) 328, 527, 601
BseRI GAGGAG 1 cut(s) 173
BseXI GCAGC 1 cut(s) 658
BsgI GTGCAG 2 cut(s) 75, 558
BshNI GGYRCC 1 cut(s) 310
BsiHKAI GWGCWC 1 cut(s) 551
BslFI GGGAC 1 cut(s) 46
BslI CCNNNNNNNGG 2 cut(s) 88, 89
BsmAI GTCTC 3 cut(s) 379, 437, 462
BsmFI GGGAC 1 cut(s) 46
BsmI GAATGC 1 cut(s) 114
Bsp1286I GDGCHC 1 cut(s) 551
Bsp1407I TGTACA 1 cut(s) 284
Bsp143I GATC 1 cut(s) 424
Bsp19I CCATGG 1 cut(s) 678
BspACI CCGC 1 cut(s) 8
BspCNI CTCAG 1 cut(s) 472
BspLI GGNNCC 3 cut(s) 301, 312, 426
BspPI GGATC 2 cut(s) 419, 432
BspT107I GGYRCC 1 cut(s) 310
BsrDI GCAATG 1 cut(s) 55
BsrGI TGTACA 1 cut(s) 284
BsrI ACTGG 3 cut(s) 328, 527, 601
BssECI CCNNGG 1 cut(s) 678
BssMI GATC 1 cut(s) 424
BssSI CACGAG 1 cut(s) 544
BssT1I CCWWGG 1 cut(s) 678
Bst2BI CACGAG 1 cut(s) 544
Bst2UI CCWGG 2 cut(s) 245, 315
Bst4CI ACNGT 2 cut(s) 310, 590
BstAUI TGTACA 1 cut(s) 284
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 2 cut(s) 453, 459
BstDSI CCRYGG 1 cut(s) 678
BstF5I GGATG 1 cut(s) 394
BstKTI GATC 1 cut(s) 427
BstMAI GTCTC 3 cut(s) 379, 437, 462
BstMBI GATC 1 cut(s) 424
BstMWI GCNNNNNNNGC 1 cut(s) 186
BstNI CCWGG 2 cut(s) 245, 315
BstSCI CCNGG 2 cut(s) 243, 313
BstSFI CTRYAG 1 cut(s) 190
BstV1I GCAGC 1 cut(s) 658
BstX2I RGATCY 1 cut(s) 424
BstYI RGATCY 1 cut(s) 424
BtgI CCRYGG 1 cut(s) 678
BtsCI GGATG 1 cut(s) 394
BtsI GCAGTG 1 cut(s) 63
BtsIMutI CAGTG 5 cut(s) 63, 318, 582, 594, 595
Cac8I GCNNGC 1 cut(s) 6
CaiI CAGNNNCTG 1 cut(s) 320
Csp6I GTAC 4 cut(s) 285, 311, 463, 698
CviAII CATG 1 cut(s) 679
CviJI RGCY 8 cut(s) 24, 189, 197, 215, 260, 356, 564, 671
CviKI_1 RGCY 8 cut(s) 24, 189, 197, 215, 260, 356, 564, 671
CviQI GTAC 4 cut(s) 285, 311, 463, 698
DdeI CTNAG 2 cut(s) 453, 459
DpnI GATC 1 cut(s) 426
DpnII GATC 1 cut(s) 424
Eco130I CCWWGG 1 cut(s) 678
Eco57I CTGAAG 1 cut(s) 534
EcoRI GAATTC 1 cut(s) 686
EcoRII CCWGG 2 cut(s) 243, 313
EcoT14I CCWWGG 1 cut(s) 678
ErhI CCWWGG 1 cut(s) 678
FaeI CATG 1 cut(s) 682
FaiI YATR 7 cut(s) 102, 192, 398, 567, 618, 680, 706
FaqI GGGAC 1 cut(s) 46
FatI CATG 1 cut(s) 678
Fnu4HI GCNGC 1 cut(s) 672
FokI GGATG 1 cut(s) 401
Fsp4HI GCNGC 1 cut(s) 672
FspBI CTAG 3 cut(s) 149, 429, 642
GluI GCNGC 1 cut(s) 672
Hin1II CATG 1 cut(s) 682
HinfI GANTC 4 cut(s) 393, 418, 472, 656
Hpy188I TCNGA 4 cut(s) 417, 483, 514, 638
Hpy188III TCNNGA 2 cut(s) 344, 422
Hpy99I CGWCG 1 cut(s) 472
HpyAV CCTTC 4 cut(s) 93, 313, 414, 551
HpyCH4III ACNGT 2 cut(s) 310, 590
HpyCH4V TGCA 3 cut(s) 56, 575, 704
HpyF10VI GCNNNNNNNGC 1 cut(s) 186
HpyF3I CTNAG 2 cut(s) 453, 459
Hsp92II CATG 1 cut(s) 682
KpnI GGTACC 1 cut(s) 314
Kzo9I GATC 1 cut(s) 424
LmnI GCTCC 1 cut(s) 668
Lsp1109I GCAGC 1 cut(s) 658
LweI GCATC 1 cut(s) 189
MaeI CTAG 3 cut(s) 149, 429, 642
MalI GATC 1 cut(s) 426
MboI GATC 1 cut(s) 424
MboII GAAGA 3 cut(s) 161, 164, 527
MflI RGATCY 1 cut(s) 424
MhlI GDGCHC 1 cut(s) 551
MluCI AATT 6 cut(s) 266, 487, 498, 504, 622, 686
MlyI GAGTC 1 cut(s) 402
MmeI TCCRAC 1 cut(s) 661
MnlI CCTC 5 cut(s) 67, 151, 154, 601, 640
MroXI GAANNNNTTC 1 cut(s) 508
MseI TTAA 2 cut(s) 209, 477
MslI CAYNNNNRTG 1 cut(s) 369
MspR9I CCNGG 2 cut(s) 245, 315
Mva1269I GAATGC 1 cut(s) 114
MvaI CCWGG 2 cut(s) 245, 315
MwoI GCNNNNNNNGC 1 cut(s) 186
NcoI CCATGG 1 cut(s) 678
NdeII GATC 1 cut(s) 424
NlaIII CATG 1 cut(s) 682
NlaIV GGNNCC 3 cut(s) 301, 312, 426
NmeAIII GCCGAG 1 cut(s) 262
PctI GAATGC 1 cut(s) 114
PdmI GAANNNNTTC 1 cut(s) 508
PfeI GAWTC 3 cut(s) 418, 472, 656
PkrI GCNGC 1 cut(s) 673
PleI GAGTC 1 cut(s) 401
PpsI GAGTC 1 cut(s) 401
Psp6I CCWGG 2 cut(s) 243, 313
PspGI CCWGG 2 cut(s) 243, 313
PspN4I GGNNCC 3 cut(s) 301, 312, 426
PstNI CAGNNNCTG 1 cut(s) 320
PsuI RGATCY 1 cut(s) 424
RsaI GTAC 4 cut(s) 286, 312, 464, 699
RsaNI GTAC 4 cut(s) 285, 311, 463, 698
RseI CAYNNNNRTG 1 cut(s) 369
SaqAI TTAA 2 cut(s) 209, 477
SatI GCNGC 1 cut(s) 672
Sau3AI GATC 1 cut(s) 424
ScaI AGTACT 1 cut(s) 464
SchI GAGTC 1 cut(s) 402
ScrFI CCNGG 2 cut(s) 245, 315
SduI GDGCHC 1 cut(s) 551
SetI ASST 8 cut(s) 191, 262, 301, 351, 566, 612, 643, 673
SfaNI GCATC 1 cut(s) 189
SfcI CTRYAG 1 cut(s) 190
SmiMI CAYNNNNRTG 1 cut(s) 369
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
SpeI ACTAGT 1 cut(s) 148
Sse9I AATT 6 cut(s) 266, 487, 498, 504, 622, 686
SsiI CCGC 1 cut(s) 8
SspMI CTAG 3 cut(s) 149, 429, 642
StyD4I CCNGG 2 cut(s) 243, 313
StyI CCWWGG 1 cut(s) 678
TaaI ACNGT 2 cut(s) 310, 590
TaqI TCGA 3 cut(s) 40, 470, 690
TasI AATT 6 cut(s) 266, 487, 498, 504, 622, 686
TatI WGTACW 2 cut(s) 284, 462
TfiI GAWTC 3 cut(s) 418, 472, 656
Tru1I TTAA 2 cut(s) 209, 477
Tru9I TTAA 2 cut(s) 209, 477
TscAI CASTG 5 cut(s) 63, 325, 589, 595, 601
TseI GCWGC 1 cut(s) 671
TspRI CASTG 5 cut(s) 63, 325, 589, 595, 601
XapI RAATTY 2 cut(s) 487, 686
XmnI GAANNNNTTC 1 cut(s) 508
XspI CTAG 3 cut(s) 149, 429, 642
ZrmI AGTACT 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.