Rmu_sc0002160.1_g000014

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002160.1
OG0010615
Physical Location & Seq
Forward (+)
70051 .. 73605
3555 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 621 bp
atgcgagagcgagactgtggccggaacttggaagagtatagtgagactgtgagacctggccagaagagatttgggatcgatcatgactgtggcctactgatagctgatgagaagctgagaactgaggatgtgggcatgtggtcgaaaaactggatttcaaatttctggtctcgatttgggatggaatcccgatttgtgaacagagagaatagggttgctgagcataaccattctctcttcccagcctcctctgccgtcaaaacacatcttcccatcgagagatctctctgtgtcttcaagacccgaatccgtggttcctctgtacatgtgttggataagaaggttgtggatgaggaggagaagcgggaggaccaccggagttccggtggaggtggtggagcattcaagccgggttctagagatgcttttgatatggctagagaaattcaggctcagtttcagagagcttccgagtcgggtaatgaaatcacaaggatgctggaggtgggaaagcatccatataatcgaaaacatggtggttacttccaagatgatatcgctagtgtcgtcgcaacttctacttccaataaggcatcatcctccgagaccggtcctgcttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

206

Amino Acids

23.23

Weight (kDa)

7.79

Isoelectric Point (pI)

43.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF632 PF04782 145 - 202 1.3e-07 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 364
AclWI GGATC 1 cut(s) 83
AcoI YGGCCR 2 cut(s) 19, 58
AcsI RAATTY 2 cut(s) 160, 444
AfaI GTAC 1 cut(s) 324
AfiI CCNNNNNNNGG 1 cut(s) 28
AflIII ACRYGT 1 cut(s) 325
AgeI ACCGGT 1 cut(s) 608
AgsI TTSAA 3 cut(s) 159, 298, 406
AjnI CCWGG 1 cut(s) 55
AluBI AGCT 3 cut(s) 104, 115, 467
AluI AGCT 3 cut(s) 104, 115, 467
Alw26I GTCTC 5 cut(s) 6, 38, 46, 174, 599
AlwI GGATC 1 cut(s) 83
AoxI GGCC 3 cut(s) 19, 58, 91
ApoI RAATTY 2 cut(s) 160, 444
ArsI GACNNNNNNTTYG 2 cut(s) 152, 184
AsiGI ACCGGT 1 cut(s) 608
AspS9I GGNCC 2 cut(s) 370, 611
AsuC2I CCSGG 1 cut(s) 411
AvaII GGWCC 2 cut(s) 370, 611
BalI TGGCCA 1 cut(s) 60
BbsI GAAGAC 1 cut(s) 286
BccI CCATC 2 cut(s) 175, 281
BceAI ACGGC 1 cut(s) 239
BciT130I CCWGG 1 cut(s) 57
BcnI CCSGG 1 cut(s) 411
BcoDI GTCTC 5 cut(s) 6, 38, 46, 174, 599
BfaI CTAG 3 cut(s) 417, 438, 561
BglII AGATCT 1 cut(s) 281
BlpI GCTNAGC 1 cut(s) 219
Bme1390I CCNGG 2 cut(s) 57, 411
Bme18I GGWCC 2 cut(s) 370, 611
BmgT120I GGNCC 2 cut(s) 370, 611
BmiI GGNNCC 1 cut(s) 316
BmrFI CCNGG 2 cut(s) 57, 411
BmsI GCATC 4 cut(s) 412, 486, 523, 602
BpiI GAAGAC 1 cut(s) 286
BpmI CTGGAG 1 cut(s) 521
Bpu1102I GCTNAGC 1 cut(s) 219
BpuMI CCSGG 1 cut(s) 411
Bsa29I ATCGAT 1 cut(s) 78
BsaI GGTCTC 3 cut(s) 46, 174, 599
BsaJI CCNNGG 1 cut(s) 310
BsaWI WCCGGW 3 cut(s) 375, 383, 608
BsaXI ACNNNNNCTCC 2 cut(s) 370, 400
Bsc4I CCNNNNNNNGG 1 cut(s) 28
Bse118I RCCGGY 1 cut(s) 608
Bse1I ACTGG 1 cut(s) 155
BseBI CCWGG 1 cut(s) 57
BseCI ATCGAT 1 cut(s) 78
BseDI CCNNGG 1 cut(s) 310
BseGI GGATG 6 cut(s) 133, 186, 355, 501, 514, 596
BseLI CCNNNNNNNGG 1 cut(s) 28
BseMII CTCAG 4 cut(s) 107, 114, 210, 467
BseNI ACTGG 1 cut(s) 155
BseRI GAGGAG 3 cut(s) 238, 368, 371
BseYI CCCAGC 1 cut(s) 241
BshFI GGCC 3 cut(s) 21, 60, 93
BshTI ACCGGT 1 cut(s) 608
BshVI ATCGAT 1 cut(s) 78
BsiSI CCGG 5 cut(s) 22, 376, 384, 410, 609
BslI CCNNNNNNNGG 1 cut(s) 28
BsmAI GTCTC 5 cut(s) 6, 38, 46, 174, 599
BsmI GAATGC 1 cut(s) 401
BsnI GGCC 3 cut(s) 21, 60, 93
Bso31I GGTCTC 3 cut(s) 46, 174, 599
Bsp1407I TGTACA 1 cut(s) 322
Bsp143I GATC 3 cut(s) 75, 79, 281
Bsp1720I GCTNAGC 1 cut(s) 219
BspACI CCGC 1 cut(s) 364
BspANI GGCC 3 cut(s) 21, 60, 93
BspCNI CTCAG 4 cut(s) 108, 115, 211, 466
BspDI ATCGAT 1 cut(s) 78
BspHI TCATGA 1 cut(s) 82
BspLI GGNNCC 1 cut(s) 316
BspPI GGATC 1 cut(s) 83
BspTNI GGTCTC 3 cut(s) 46, 174, 599
BsrFI RCCGGY 1 cut(s) 608
BsrGI TGTACA 1 cut(s) 322
BsrI ACTGG 1 cut(s) 155
BssAI RCCGGY 1 cut(s) 608
BssECI CCNNGG 1 cut(s) 310
BssMI GATC 3 cut(s) 75, 79, 281
Bst2UI CCWGG 1 cut(s) 57
Bst4CI ACNGT 3 cut(s) 17, 49, 89
Bst6I CTCTTC 3 cut(s) 27, 59, 242
BstAUI TGTACA 1 cut(s) 322
BstDEI CTNAG 5 cut(s) 116, 123, 219, 453, 618
BstDSI CCRYGG 1 cut(s) 310
BstF5I GGATG 6 cut(s) 133, 186, 355, 501, 514, 596
BstKTI GATC 3 cut(s) 78, 82, 284
BstMAI GTCTC 5 cut(s) 6, 38, 46, 174, 599
BstMBI GATC 3 cut(s) 75, 79, 281
BstMWI GCNNNNNNNGC 1 cut(s) 251
BstNI CCWGG 1 cut(s) 57
BstNSI RCATGY 2 cut(s) 139, 329
BstSCI CCNGG 2 cut(s) 55, 409
BstV2I GAAGAC 1 cut(s) 286
BstX2I RGATCY 1 cut(s) 281
BstYI RGATCY 1 cut(s) 281
Bsu15I ATCGAT 1 cut(s) 78
BsuRI GGCC 3 cut(s) 21, 60, 93
BsuTUI ATCGAT 1 cut(s) 78
BtgI CCRYGG 1 cut(s) 310
BtsCI GGATG 6 cut(s) 133, 186, 355, 501, 514, 596
CciI TCATGA 1 cut(s) 82
Cfr10I RCCGGY 1 cut(s) 608
Cfr13I GGNCC 2 cut(s) 370, 611
ClaI ATCGAT 1 cut(s) 78
Csp6I GTAC 1 cut(s) 323
CspAI ACCGGT 1 cut(s) 608
CviAII CATG 4 cut(s) 83, 136, 326, 533
CviQI GTAC 1 cut(s) 323
DdeI CTNAG 5 cut(s) 116, 123, 219, 453, 618
DpnI GATC 3 cut(s) 77, 81, 283
DpnII GATC 3 cut(s) 75, 79, 281
EaeI YGGCCR 2 cut(s) 19, 58
Eam1104I CTCTTC 3 cut(s) 27, 59, 242
EarI CTCTTC 3 cut(s) 27, 59, 242
Eco31I GGTCTC 3 cut(s) 46, 174, 599
Eco32I GATATC 1 cut(s) 556
Eco47I GGWCC 2 cut(s) 370, 611
EcoRII CCWGG 1 cut(s) 55
EcoRV GATATC 1 cut(s) 556
FaeI CATG 4 cut(s) 86, 139, 329, 536
FaiI YATR 9 cut(s) 39, 84, 137, 225, 327, 434, 520, 522, 534
FatI CATG 4 cut(s) 82, 135, 325, 532
FauI CCCGC 1 cut(s) 357
FokI GGATG 6 cut(s) 140, 193, 362, 501, 508, 583
FspBI CTAG 3 cut(s) 417, 438, 561
GsaI CCCAGC 1 cut(s) 245
GsuI CTGGAG 1 cut(s) 521
HaeIII GGCC 3 cut(s) 21, 60, 93
HapII CCGG 5 cut(s) 22, 376, 384, 410, 609
Hin1II CATG 4 cut(s) 86, 139, 329, 536
HinfI GANTC 3 cut(s) 185, 306, 473
HpaII CCGG 5 cut(s) 22, 376, 384, 410, 609
Hpy166II GTNNAC 1 cut(s) 199
Hpy188I TCNGA 3 cut(s) 462, 472, 604
Hpy188III TCNNGA 6 cut(s) 83, 171, 189, 277, 298, 417
Hpy8I GTNNAC 1 cut(s) 199
Hpy99I CGWCG 1 cut(s) 572
HpyAV CCTTC 1 cut(s) 334
HpyCH4III ACNGT 3 cut(s) 17, 49, 89
HpyF10VI GCNNNNNNNGC 1 cut(s) 251
HpyF3I CTNAG 5 cut(s) 116, 123, 219, 453, 618
Hsp92II CATG 4 cut(s) 86, 139, 329, 536
Kzo9I GATC 3 cut(s) 75, 79, 281
LmnI GCTCC 1 cut(s) 398
LweI GCATC 4 cut(s) 412, 486, 523, 602
MaeI CTAG 3 cut(s) 417, 438, 561
MaeIII GTNAC 1 cut(s) 539
MalI GATC 3 cut(s) 77, 81, 283
MboI GATC 3 cut(s) 75, 79, 281
MboII GAAGA 5 cut(s) 44, 76, 229, 260, 286
MflI RGATCY 1 cut(s) 281
MlsI TGGCCA 1 cut(s) 60
MluCI AATT 2 cut(s) 160, 444
MluNI TGGCCA 1 cut(s) 60
MlyI GAGTC 1 cut(s) 482
MmeI TCCRAC 1 cut(s) 312
Mox20I TGGCCA 1 cut(s) 60
MscI TGGCCA 1 cut(s) 60
MslI CAYNNNNRTG 2 cut(s) 87, 494
Msp20I TGGCCA 1 cut(s) 60
MspI CCGG 5 cut(s) 22, 376, 384, 410, 609
MspR9I CCNGG 2 cut(s) 57, 411
Mva1269I GAATGC 1 cut(s) 401
MvaI CCWGG 1 cut(s) 57
MwoI GCNNNNNNNGC 1 cut(s) 251
NciI CCSGG 1 cut(s) 411
NdeII GATC 3 cut(s) 75, 79, 281
NlaIII CATG 4 cut(s) 86, 139, 329, 536
NlaIV GGNNCC 1 cut(s) 316
NspI RCATGY 2 cut(s) 139, 329
PagI TCATGA 1 cut(s) 82
PciI ACATGT 1 cut(s) 325
PcsI WCGNNNNNNNCGW 1 cut(s) 564
PctI GAATGC 1 cut(s) 401
PfeI GAWTC 2 cut(s) 185, 306
PinAI ACCGGT 1 cut(s) 608
PleI GAGTC 1 cut(s) 481
PpsI GAGTC 1 cut(s) 481
PscI ACATGT 1 cut(s) 325
Psp6I CCWGG 1 cut(s) 55
PspFI CCCAGC 1 cut(s) 241
PspGI CCWGG 1 cut(s) 55
PspN4I GGNNCC 1 cut(s) 316
PspPI GGNCC 2 cut(s) 370, 611
PsuI RGATCY 1 cut(s) 281
RsaI GTAC 1 cut(s) 324
RsaNI GTAC 1 cut(s) 323
RseI CAYNNNNRTG 2 cut(s) 87, 494
Sau3AI GATC 3 cut(s) 75, 79, 281
Sau96I GGNCC 2 cut(s) 370, 611
SchI GAGTC 1 cut(s) 482
ScrFI CCNGG 2 cut(s) 57, 411
SetI ASST 7 cut(s) 58, 106, 117, 345, 394, 469, 507
SfaNI GCATC 4 cut(s) 412, 486, 523, 602
SinI GGWCC 2 cut(s) 370, 611
SmiMI CAYNNNNRTG 2 cut(s) 87, 494
Sse9I AATT 2 cut(s) 160, 444
SsiI CCGC 1 cut(s) 364
SspMI CTAG 3 cut(s) 417, 438, 561
StyD4I CCNGG 2 cut(s) 55, 409
TaaI ACNGT 3 cut(s) 17, 49, 89
TaqI TCGA 5 cut(s) 78, 143, 172, 276, 526
TasI AATT 2 cut(s) 160, 444
TatI WGTACW 1 cut(s) 322
TfiI GAWTC 2 cut(s) 185, 306
TspDTI ATGAA 1 cut(s) 498
TspGWI ACGGA 1 cut(s) 299
VpaK11BI GGWCC 2 cut(s) 370, 611
XapI RAATTY 2 cut(s) 160, 444
XbaI TCTAGA 1 cut(s) 416
XceI RCATGY 2 cut(s) 139, 329
XcmI CCANNNNNNNNNTGG 1 cut(s) 68
XspI CTAG 3 cut(s) 417, 438, 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.