Rmu_sc0002380.1_g000009

Callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002380.1
OG0005959
Physical Location & Seq
Forward (+)
44306 .. 59005
14700 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5931 bp
atgtcgtcgtcgagagcggggccggatcaggggccgccgccgcagcggcgtatccagcggacgcagacagctggaaacctcggggaggcggctttcgacagtgaggtggtgccgtcatcgctagtcgaaatcgctcccattcttcgtgtggccaacgaggtggaatcgcacaatcccagagttgcttatctatgtcgattttatgcctttgagaaagctcataggttggatcccacttctagtggacgtggtgttcgtcagttcaaaactgctcttcttcaacgtcttgaaagggagaatgatccaactcttatgggaagggtaaaaaaaagtgatgcacgtgaaatgcagagcttctatcaacactattacaaaaaatatattcaagctttgcaaaatgcagccgataaagctgaccgtgcacaacttaccaaggcataccagactgccaatgttctatttgaggttttaaaggctgttaatatgacacaatctatggaagttgatcgtgagattttggaagctcatgataaagttgcagaaaagacagagctattggttccttacaatatccttccacttgatcctgatagtgtaaatcaagcaattatgagatacccagagattcaagctgctgttgttgctcttcgtaacaccaggggtcttccttggcctaaggaatacaaaaagaaaaaagacgaggatgttcttgattggctgcagtcaatgtttgggtttcagaaggacaatgtggcgaatcaacgggagcacctgatattgttacttgcaaatgtgcacataaggcagtttccaaagcctgatcaacagcccaagttggatgaccgtgccctgacagaagtgatgaagaagcttttcaagaattacaaaaaatggtgcaagttcttgggccggaaaagtagcctttggttaccaaccatacagcaagaggtgcaacagcgcaagctgctttacatgggtctatatcttttgatatggggggaagctgcaaatttgagattcatgccagaatgcctttgttacatttatcatcatatggcatttgaattgtatggtatgttagctgggaatgtgagtccaatgacaggagaaaatgtgaagccagcctatggaggtgaagaagaggctttcttgaaaaaagttgtgactcctatctataacgtgattgccagggaagctgataggagcaaaagaggggaatcaaagcattcccagtggaggaactatgatgatataaatgaatacttttggtcagtggattgtttccggttgggttggccgatgcgtgcagatgctgatttcttttgctgctctagtgaacaacgtcattttgataaaaatagtgaggataacaaaccagctagtggagatcgatgggtggggaaagttaactttgttgagatacggtcattctggcatatctttagaagctttgaccgcatgtggagtttctttattctatgtttacaggttatgataattgttgcttggaatggctcgggccaacccacatcaatattttctgccgatgtattcaagaaagctttgagtgtctttataactgctgcaatattgaagcttggacaagctgttctagatgtaattcttagctggaagtctaggcggagtatgtcattccatgttaagttgagatatattgctaaggtcatttcagctgctgcgtgggtaataattctaccggttacttatgcgtatacttgggaaaaccctcctgggtttgctcagaccattaaaggttggtttggcaatagtaaaaacgcgccctccttgttcatcttggctgttgttatctacttgtcaccgaatatgctggctggggtgttgtttcttttcccatttattcgccgattccttgagagatcaaactataggattgtgatgcttatgatgtggtggtcacagcctcgtctctatgttgggaggggaatgcatgagagcacattttctcttttcaagtacacgatgttttgggttctccttgtagtcacaaagttggcattcagttactatatagagataaagcctttagtgggtccaacaaaagccatcatgaatgtacgtataactaatttccagtggcatgagttctttccccgtgcaaagaacaatatcggtgttgtgattgcactctgggctccaattatcctggtctattttatggatacccagatttggtatgcaatatactccacaatattcggaggaatttatggtgcattccgtcgccttggagagatacggacactaggaatgctaagatctcgctttgaatcattgcctggtgcttttaacgcccgtttaattccagcggacaagagtgagccaaagaagaaaggactgaaggctacattgtcccgcacctttggtcatgttgaaggtagtaaagacaaacaggctgcaaggtttgcgcaattatggaacaaaataataagtagtttcagagaggaggatcttataaaccaaagggagatgaaccttttgcttgttccttattgggctgatcgtgaaatggacctcatacggtggcctccatttttacttgctagcaagatcccgatagcattagacatggccaaggacagcaatggcaaggataaagagctaacaaaaagaatcctggctgatgaatacatgcattgtgctgttcgcgaatgctatgcttcatttaagaacataattaagtttctggttcagggggaccgtgagaaagaggttataaattccatattttctgaggttgacaagcacatagaagatggtgacctgatccgtgaattgaagatgagtgctcttcctagcctctatgaccactttgttcgccttatcaactttttgagcaagaatagtcaggacgacagggatcaagttgtgattctattccaggacatgctggaggtagtgactagagatataatgatggaggagcagataaactgcttggtagattcagtccacggaggctcgggccatgaggggatgatgccccttgatcaacatcaacagcatcagttgtttgcatctgctggagctatcaactttccacttacacatgtaacagaggcttggaaggagaagattaaccgtctttttctattacttaccacaaatcttacacatgtaacagaggcttggaaggagaagattaaccgtctttttctattacttaccacaaaggagtctgccatggacgtgccatccaacttagaagctagaaggcgaatttctttcttctcgaattcattgtttatggatatgcctccagcaccaaaagttcgcaatatgctttctttctctgttttaactccttactacaacgaggaggttctcttttccattgacggtctggaaaaaccgaatgaagatggtgtttctattctcttctacttgcaaaagattttcccagatgaatggaacaactttcttgagcgtgtgaaatgcagcaacgaagaggaactaaaaggttctgatgaattggaagaagaacttcgcctatgggcatcatatagaggccaaactttgactcgtactgtaagagggatgatgtactaccggaaagctttggagcttcaggcttttctggatatggccaaagaggaggatttaatggaaggctataaggccatagagttgaattcagaagatcaatcaaaggagggaagatcactatgggcgcaatgtcaagcagtagctgatatgaaattcacatatgtggtttcatgccaaaagtatggtattcagaagcgatctggtgatcttcgtgcacaggacattctgaggctcatgacaacatacccatcattacgagtggcatatattgatgaggttgaagaacctagcaaagataggtctcagaagataaaccaaaaggcttattattctactttggtaaaggctgctatgccaaagtcaattgacccttcagagccagtacaaaatcttgaccaggttatttatcgcataaagcttccaggacctgctattttgggagagggaaagccagaaaatcaaaatcatgccattattttcacgcgtggagaaggcttgcaaacaattgacatgaaccaggataactacatggaagaagctttgaaaatgaggaatttgctgcaagaatttcttaagcatgatggcgtgaggcaccctacaattcttggacttagggagcatatatttacaggaagtgtttcttctctagcttggttcatgtcaaatcaggagaacagttttgtgactattggtcaaagactattggccaacccactcagggttcgatttcattatgggcatcccgatgtatttgataggctctttcaccttagtagagggggtgttagtaaggcatccaaggttatcaatttgagtgaagacatttttgctggctttaattccactctccgtgaaggcaatgtaactcatcacgaatacatacaagtggggaaggggagagatgtcggtcttaaccaaatctccatgtttgaggcaaaaattgctaatggcaacggggagcagacactcagtcgagatatataccgacttggacatcgttttgactttttccgtatgttgtcatgttatttcaccacaattggtttctacttcagtactctgatcactgtgcttacagtgtatgtcttcctttatggtcgcctctacctggttcttagtggacttgaagaaggtttgaacactcaagcagcaattcgagataataagcctcttcaagttgctctcgcttctcaatcatttgttcaaatagggtttttgatggctttgcctatgttgatggaaattggcttggaaaagggtttccgaactgcattaagtgaattcgtgttgatgcaattgcaactggccccagtatttttcacattctcacttgggacaaagacccactattatggacggacattactgcatggtggtgccaaatatagatctacaggtcgtgggtttgtggtgttccatgccaagtttgccgacaactataggctctactcccgcagccactttgttaagggtattgagctcttgattttacttgtggtgtaccagatctttggtcatacttacagaagtgctgttgcctacattttgatcactgtatctatgtggtttatggtggttacctggctttttgctccctttctgttcaatccatctggttttgagtggcaaaagattgttgatgattggactgattggaataagtggataagcaaccgtggaggtataggtgttccgcctgaaaaaagttgggaatcatggtgggaggaggaacaagaacatcttcgatattccggaaaacgtggcattgtagttgagatactgctatctgtacgattctttatctatcagtatgggcttgtatatcacttaaacattgcaaagaaaaccaagagtgtcctggtctatggtatctcatggctggtgatcgtccttattttgtttgtcatgaagactgtatctgttggtaggagaaagttcagtgctgagtaccaacttgtattccggctgatcaagggattaatattcattacttttgtatccattttggtcactttgattgtgctgcctcacatgaccctgcaggacatcattgtttgcattcttgctttcatgccaactggttgggggatgcttatgattgcccaagcttgcaaaggcttggttcacaaagctggtttatggccatcagttcggactcttgcccgtggctttgagatagttatgggcttgcttctgttcaccccagttgcattcttggcctggtttccttttgtttcagaatttcaaactcgtatgctcttcaaccaagcattcagtagaggtttacagatttctcgtattcttggcggacaacgcaaggatcgttcatcccggaacaaggagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1976

Amino Acids

228.13

Weight (kDa)

9.19

Isoelectric Point (pI)

41.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 44 - 171 1.9e-17 Vta1 like
FKS1_dom1 PF14288 321 - 433 3.4e-43 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 856 - 1048 2.3e-65 Callose synthase 1 domain
Glucan_synthase PF02364 1077 - 1870 4.5e-239 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1577, 2495, 2756
Acc16I TGCGCA 1 cut(s) 2448
Acc36I ACCTGC 1 cut(s) 4021
AccB1I GGYRCC 3 cut(s) 109, 4176, 4961
AccB7I CCANNNNNTGG 2 cut(s) 1127, 1382
AccBSI CCGCTC 1 cut(s) 17
AccI GTMKAC 1 cut(s) 1733
AccII CGCG 3 cut(s) 1799, 2688, 4069
AccIII TCCGGA 1 cut(s) 5336
AcoI YGGCCR 6 cut(s) 150, 1295, 2610, 3624, 4290, 5726
AcuI CTGAAG 4 cut(s) 2399, 3590, 3942, 4621
AcvI CACGTG 1 cut(s) 341
AfaI GTAC 9 cut(s) 1997, 2097, 3565, 3584, 3969, 4642, 5087, 5376, 5534
AflII CTTAAG 1 cut(s) 4157
AflIII ACRYGT 3 cut(s) 3088, 3154, 4067
AgeI ACCGGT 1 cut(s) 1717
AhdI GACNNNNNGTC 2 cut(s) 4275, 4554
AjiI CACGTC 2 cut(s) 248, 3229
AleI CACNNNNGTG 1 cut(s) 3746
AloI GAACNNNNNNTCC 4 cut(s) 237, 269, 1593, 1625
Alw21I GWGCWC 7 cut(s) 424, 771, 798, 1979, 2830, 3802, 5067
Alw26I GTCTC 2 cut(s) 1952, 3891
Alw44I GTGCAC 3 cut(s) 420, 794, 3798
AlwNI CAGNNNCTG 4 cut(s) 1313, 1697, 3728, 4013
Ama87I CYCGRG 3 cut(s) 80, 1516, 3001
Aor13HI TCCGGA 1 cut(s) 5336
ApaLI GTGCAC 3 cut(s) 420, 794, 3798
ArsI GACNNNNNNTTYG 2 cut(s) 237, 269
AseI ATTAAT 1 cut(s) 5564
AsiGI ACCGGT 1 cut(s) 1717
Asp700I GAANNNNTTC 4 cut(s) 872, 3522, 4222, 5325
AspLEI GCGC 4 cut(s) 960, 1801, 2449, 3712
AsuC2I CCSGG 1 cut(s) 5917
AsuHPI GGTGA 8 cut(s) 1145, 1830, 2810, 3800, 4343, 4609, 5479, 5776
AsuNHI GCTAGC 1 cut(s) 2582
AvaI CYCGRG 3 cut(s) 80, 1516, 3001
AvaII GGWCC 4 cut(s) 2072, 2551, 2737, 4010
AxyI CCTNAGG 1 cut(s) 675
BaeGI GKGCMC 4 cut(s) 424, 798, 850, 3802
BaeI ACNNNNGTAYC 2 cut(s) 5565, 5598
BalI TGGCCA 5 cut(s) 152, 2612, 3626, 4292, 5728
BamHI GGATCC 1 cut(s) 229
BanI GGYRCC 3 cut(s) 109, 4176, 4961
BanII GRGCYC 2 cut(s) 2176, 5067
BbrPI CACGTG 1 cut(s) 341
BbsI GAAGAC 4 cut(s) 656, 4410, 4663, 5501
Bbv12I GWGCWC 7 cut(s) 424, 771, 798, 1979, 2830, 3802, 5067
BceAI ACGGC 1 cut(s) 97
BcgI CGANNNNNNTGC 2 cut(s) 2678, 2712
BciVI GTATCC 3 cut(s) 62, 2194, 5593
BclI TGATCA 5 cut(s) 820, 3028, 4647, 5133, 5553
BcnI CCSGG 1 cut(s) 5917
BcoDI GTCTC 2 cut(s) 1952, 3891
BfmI CTRYAG 5 cut(s) 719, 1906, 4977, 5023, 5624
BfrI CTTAAG 1 cut(s) 4157
BfuAI ACCTGC 1 cut(s) 4021
BfuI GTATCC 3 cut(s) 62, 2194, 5593
BglI GCCNNNNNGGC 1 cut(s) 46
BglII AGATCT 3 cut(s) 2297, 4973, 5091
BmcAI AGTACT 1 cut(s) 4642
Bme18I GGWCC 4 cut(s) 2072, 2551, 2737, 4010
BmeRI GACNNNNNGTC 2 cut(s) 4275, 4554
BmeT110I CYCGRG 3 cut(s) 80, 1516, 3001
BmgBI CACGTC 2 cut(s) 248, 3229
BmrI ACTGGG 3 cut(s) 1225, 4889, 5783
BmtI GCTAGC 1 cut(s) 2586
BmuI ACTGGG 3 cut(s) 1225, 4889, 5783
BpiI GAAGAC 4 cut(s) 656, 4410, 4663, 5501
BpmI CTGGAG 3 cut(s) 2951, 3084, 3282
Bpu10I CCTNAGC 1 cut(s) 1680
BpuEI CTTGAG 3 cut(s) 1913, 3482, 4713
BpuMI CCSGG 1 cut(s) 5917
Bsa29I ATCGAT 1 cut(s) 1390
BsaAI YACGTR 2 cut(s) 341, 2099
BsaBI GATNNNNATC 1 cut(s) 3033
BsaI GGTCTC 1 cut(s) 3891
BsaWI WCCGGW 4 cut(s) 1284, 1717, 3588, 5336
BsaXI ACNNNNNCTCC 6 cut(s) 1098, 1128, 1787, 1817, 4490, 4520
Bse118I RCCGGY 1 cut(s) 1717
Bse1I ACTGG 7 cut(s) 1231, 2113, 3965, 4893, 4895, 5668, 5789
Bse21I CCTNAGG 1 cut(s) 675
Bse3DI GCAATG 5 cut(s) 2312, 2629, 3719, 4450, 5418
Bse8I GATNNNNATC 1 cut(s) 3033
BseAI TCCGGA 1 cut(s) 5336
BseCI ATCGAT 1 cut(s) 1390
BseGI GGATG 9 cut(s) 709, 844, 3021, 3233, 3582, 4324, 4379, 5679, 5912
BseJI GATNNNNATC 1 cut(s) 3033
BseMI GCAATG 5 cut(s) 2312, 2629, 3719, 4450, 5418
BseMII CTCAG 7 cut(s) 1775, 2763, 3803, 3902, 4315, 4567, 5520
BseNI ACTGG 7 cut(s) 1231, 2113, 3965, 4893, 4895, 5668, 5789
BseRI GAGGAG 5 cut(s) 2498, 2975, 3371, 3647, 5324
BseSI GKGCMC 4 cut(s) 424, 798, 850, 3802
BseYI CCCAGC 2 cut(s) 1082, 1853
BsgI GTGCAG 1 cut(s) 1326
Bsh1236I CGCG 3 cut(s) 1799, 2688, 4069
BshNI GGYRCC 3 cut(s) 109, 4176, 4961
BshTI ACCGGT 1 cut(s) 1717
BshVI ATCGAT 1 cut(s) 1390
BsiHKAI GWGCWC 7 cut(s) 424, 771, 798, 1979, 2830, 3802, 5067
BsiHKCI CYCGRG 3 cut(s) 80, 1516, 3001
BsiSI CCGG 8 cut(s) 23, 910, 1285, 1718, 3589, 5337, 5548, 5917
BslFI GGGAC 3 cut(s) 2377, 2750, 4933
BsmAI GTCTC 2 cut(s) 1952, 3891
BsmBI CGTCTC 1 cut(s) 1952
BsmFI GGGAC 3 cut(s) 2377, 2750, 4933
Bso31I GGTCTC 1 cut(s) 3891
BsoBI CYCGRG 3 cut(s) 80, 1516, 3001
Bsp1286I GDGCHC 9 cut(s) 424, 771, 798, 850, 1979, 2176, 2830, 3802, 5067
Bsp13I TCCGGA 1 cut(s) 5336
Bsp19I CCATGG 1 cut(s) 3222
Bsp68I TCGCGA 1 cut(s) 2688
BspCNI CTCAG 7 cut(s) 1774, 2764, 3804, 3901, 4314, 4566, 5521
BspDI ATCGAT 1 cut(s) 1390
BspEI TCCGGA 1 cut(s) 5336
BspFNI CGCG 3 cut(s) 1799, 2688, 4069
BspHI TCATGA 4 cut(s) 526, 2088, 3819, 5490
BspMAI CTGCAG 2 cut(s) 723, 5628
BspMI ACCTGC 1 cut(s) 4021
BspOI GCTAGC 1 cut(s) 2586
BspQI GCTCTTC 4 cut(s) 279, 651, 2835, 5849
BspT107I GGYRCC 3 cut(s) 109, 4176, 4961
BspTI CTTAAG 1 cut(s) 4157
BspTNI GGTCTC 1 cut(s) 3891
BsrBI CCGCTC 1 cut(s) 17
BsrDI GCAATG 5 cut(s) 2312, 2629, 3719, 4450, 5418
BsrFI RCCGGY 1 cut(s) 1717
BsrI ACTGG 7 cut(s) 1231, 2113, 3965, 4893, 4895, 5668, 5789
BssAI RCCGGY 1 cut(s) 1717
BssNAI GTATAC 1 cut(s) 1734
BssT1I CCWWGG 6 cut(s) 432, 668, 2266, 2613, 3222, 4383
Bst1107I GTATAC 1 cut(s) 1734
Bst6I CTCTTC 8 cut(s) 279, 651, 1134, 2835, 3422, 3480, 4761, 5849
BstAFI CTTAAG 1 cut(s) 4157
BstAPI GCANNNNNTGC 3 cut(s) 949, 2444, 4526
BstBAI YACGTR 2 cut(s) 341, 2099
BstC8I GCNNGC 9 cut(s) 962, 1122, 1305, 1851, 2584, 4082, 4417, 5695, 5774
BstDSI CCRYGG 4 cut(s) 2992, 3222, 5260, 5749
BstEII GGTNACC 3 cut(s) 927, 2798, 5161
BstENI CCTNNNNNAGG 1 cut(s) 83
BstF5I GGATG 9 cut(s) 709, 844, 3021, 3233, 3582, 4324, 4379, 5679, 5912
BstFNI CGCG 3 cut(s) 1799, 2688, 4069
BstHHI GCGC 4 cut(s) 960, 1801, 2449, 3712
BstMAI GTCTC 2 cut(s) 1952, 3891
BstNSI RCATGY 5 cut(s) 1462, 2674, 2929, 3092, 3158
BstPI GGTNACC 3 cut(s) 927, 2798, 5161
BstSFI CTRYAG 5 cut(s) 719, 1906, 4977, 5023, 5624
BstSLI GKGCMC 4 cut(s) 424, 798, 850, 3802
BstSNI TACGTA 1 cut(s) 2099
BstUI CGCG 3 cut(s) 1799, 2688, 4069
BstV2I GAAGAC 4 cut(s) 656, 4410, 4663, 5501
BstX2I RGATCY 6 cut(s) 229, 2297, 2488, 2589, 4973, 5091
BstXI CCANNNNNNTGG 6 cut(s) 160, 3447, 3767, 4937, 5096, 5667
BstYI RGATCY 6 cut(s) 229, 2297, 2488, 2589, 4973, 5091
BstZ17I GTATAC 1 cut(s) 1734
Bsu15I ATCGAT 1 cut(s) 1390
Bsu36I CCTNAGG 1 cut(s) 675
BsuI GTATCC 3 cut(s) 62, 2194, 5593
BsuTUI ATCGAT 1 cut(s) 1390
BtgI CCRYGG 4 cut(s) 2992, 3222, 5260, 5749
BtgZI GCGATG 1 cut(s) 102
BtrI CACGTC 2 cut(s) 248, 3229
BtsCI GGATG 9 cut(s) 709, 844, 3021, 3233, 3582, 4324, 4379, 5679, 5912
BtsIMutI CAGTG 8 cut(s) 106, 1238, 1278, 2120, 4650, 4668, 5136, 5530
BtuMI TCGCGA 1 cut(s) 2688
BveI ACCTGC 1 cut(s) 4021
Cac8I GCNNGC 9 cut(s) 962, 1122, 1305, 1851, 2584, 4082, 4417, 5695, 5774
CaiI CAGNNNCTG 4 cut(s) 1313, 1697, 3728, 4013
CciI TCATGA 4 cut(s) 526, 2088, 3819, 5490
CfoI GCGC 4 cut(s) 960, 1801, 2449, 3712
Cfr10I RCCGGY 1 cut(s) 1717
ClaI ATCGAT 1 cut(s) 1390
CseI GACGC 1 cut(s) 70
CsiI ACCWGGT 2 cut(s) 3981, 4692
Csp6I GTAC 9 cut(s) 1996, 2096, 3564, 3583, 3968, 4641, 5086, 5375, 5533
CspAI ACCGGT 1 cut(s) 1717
CviQI GTAC 9 cut(s) 1996, 2096, 3564, 3583, 3968, 4641, 5086, 5375, 5533
DraI TTTAAA 1 cut(s) 471
DriI GACNNNNNGTC 2 cut(s) 4275, 4554
EaeI YGGCCR 6 cut(s) 150, 1295, 2610, 3624, 4290, 5726
Eam1104I CTCTTC 8 cut(s) 279, 651, 1134, 2835, 3422, 3480, 4761, 5849
Eam1105I GACNNNNNGTC 2 cut(s) 4275, 4554
EarI CTCTTC 8 cut(s) 279, 651, 1134, 2835, 3422, 3480, 4761, 5849
EciI GGCGGA 3 cut(s) 1657, 5268, 5907
Ecl136II GAGCTC 1 cut(s) 5065
Eco105I TACGTA 1 cut(s) 2099
Eco130I CCWWGG 6 cut(s) 432, 668, 2266, 2613, 3222, 4383
Eco24I GRGCYC 2 cut(s) 2176, 5067
Eco31I GGTCTC 1 cut(s) 3891
Eco47I GGWCC 4 cut(s) 2072, 2551, 2737, 4010
Eco53kI GAGCTC 1 cut(s) 5065
Eco57I CTGAAG 4 cut(s) 2399, 3590, 3942, 4621
Eco72I CACGTG 1 cut(s) 341
Eco81I CCTNAGG 1 cut(s) 675
Eco88I CYCGRG 3 cut(s) 80, 1516, 3001
Eco91I GGTNACC 3 cut(s) 927, 2798, 5161
EcoICRI GAGCTC 1 cut(s) 5065
EcoNI CCTNNNNNAGG 1 cut(s) 83
EcoO109I RGGNCCY 1 cut(s) 4010
EcoO65I GGTNACC 3 cut(s) 927, 2798, 5161
EcoRI GAATTC 3 cut(s) 3274, 3670, 4865
EcoT14I CCWWGG 6 cut(s) 432, 668, 2266, 2613, 3222, 4383
EcoT22I ATGCAT 2 cut(s) 1971, 2676
EcoT38I GRGCYC 2 cut(s) 2176, 5067
ErhI CCWWGG 6 cut(s) 432, 668, 2266, 2613, 3222, 4383
Esp3I CGTCTC 1 cut(s) 1952
FalI AAGNNNNNCTT 8 cut(s) 3507, 3539, 4140, 4172, 4210, 4242, 5310, 5342
FaqI GGGAC 3 cut(s) 2377, 2750, 4933
FauI CCCGC 3 cut(s) 10, 2402, 5045
FauNDI CATATG 2 cut(s) 1053, 3745
FbaI TGATCA 5 cut(s) 820, 3028, 4647, 5133, 5553
FblI GTMKAC 1 cut(s) 1733
FokI GGATG 9 cut(s) 716, 851, 3028, 3220, 3589, 4311, 4366, 5686, 5899
FriOI GRGCYC 2 cut(s) 2176, 5067
FspI TGCGCA 1 cut(s) 2448
GlaI GCGC 4 cut(s) 959, 1800, 2448, 3711
GsaI CCCAGC 2 cut(s) 1086, 1857
GsuI CTGGAG 3 cut(s) 2951, 3084, 3282
HapII CCGG 8 cut(s) 23, 910, 1285, 1718, 3589, 5337, 5548, 5917
HgaI GACGC 1 cut(s) 70
HhaI GCGC 4 cut(s) 960, 1801, 2449, 3712
Hin6I GCGC 4 cut(s) 958, 1799, 2447, 3710
HinP1I GCGC 4 cut(s) 958, 1799, 2447, 3710
HincII GTYRAC 2 cut(s) 1408, 2779
HindII GTYRAC 2 cut(s) 1408, 2779
HindIII AAGCTT 9 cut(s) 387, 869, 1447, 1560, 1595, 3594, 4001, 4122, 5691
HpaI GTTAAC 1 cut(s) 1408
HpaII CCGG 8 cut(s) 23, 910, 1285, 1718, 3589, 5337, 5548, 5917
HphI GGTGA 8 cut(s) 1145, 1830, 2810, 3800, 4343, 4609, 5479, 5776
Hpy99I CGWCG 3 cut(s) 10, 13, 2265
HpyCH4IV ACGT 8 cut(s) 247, 283, 340, 1179, 1342, 2098, 3228, 5344
HpySE526I ACGT 8 cut(s) 247, 283, 340, 1179, 1342, 2098, 3228, 5344
HspAI GCGC 4 cut(s) 958, 1799, 2447, 3710
Kpn2I TCCGGA 1 cut(s) 5336
Ksp22I TGATCA 5 cut(s) 820, 3028, 4647, 5133, 5553
KspAI GTTAAC 1 cut(s) 1408
LguI GCTCTTC 4 cut(s) 279, 651, 2835, 5849
MabI ACCWGGT 2 cut(s) 3981, 4692
MaeII ACGT 8 cut(s) 247, 283, 340, 1179, 1342, 2098, 3228, 5344
MbiI CCGCTC 1 cut(s) 17
MfeI CAATTG 4 cut(s) 3948, 4089, 4623, 4880
MflI RGATCY 6 cut(s) 229, 2297, 2488, 2589, 4973, 5091
MhlI GDGCHC 9 cut(s) 424, 771, 798, 850, 1979, 2176, 2830, 3802, 5067
MlsI TGGCCA 5 cut(s) 152, 2612, 3626, 4292, 5728
MluI ACGCGT 1 cut(s) 4067
MluNI TGGCCA 5 cut(s) 152, 2612, 3626, 4292, 5728
MlyI GAGTC 5 cut(s) 1102, 1159, 3224, 3553, 5734
MmeI TCCRAC 5 cut(s) 207, 329, 816, 2099, 3261
Mox20I TGGCCA 5 cut(s) 152, 2612, 3626, 4292, 5728
Mph1103I ATGCAT 2 cut(s) 1971, 2676
MroI TCCGGA 1 cut(s) 5336
MroXI GAANNNNTTC 4 cut(s) 872, 3522, 4222, 5325
MscI TGGCCA 5 cut(s) 152, 2612, 3626, 4292, 5728
MslI CAYNNNNRTG 6 cut(s) 3227, 3746, 4329, 4469, 4935, 4959
Msp20I TGGCCA 5 cut(s) 152, 2612, 3626, 4292, 5728
MspA1I CMGCKG 5 cut(s) 46, 58, 71, 1694, 2348
MspCI CTTAAG 1 cut(s) 4157
MspI CCGG 8 cut(s) 23, 910, 1285, 1718, 3589, 5337, 5548, 5917
MunI CAATTG 4 cut(s) 3948, 4089, 4623, 4880
MvnI CGCG 3 cut(s) 1799, 2688, 4069
NciI CCSGG 1 cut(s) 5917
NcoI CCATGG 1 cut(s) 3222
NdeI CATATG 2 cut(s) 1053, 3745
NheI GCTAGC 1 cut(s) 2582
NmuCI GTSAC 8 cut(s) 1162, 1836, 1935, 2023, 2798, 2938, 4267, 5593
NruI TCGCGA 1 cut(s) 2688
NsbI TGCGCA 1 cut(s) 2448
NsiI ATGCAT 2 cut(s) 1971, 2676
NspI RCATGY 5 cut(s) 1462, 2674, 2929, 3092, 3158
OliI CACNNNNGTG 1 cut(s) 3746
PagI TCATGA 4 cut(s) 526, 2088, 3819, 5490
PciI ACATGT 2 cut(s) 3088, 3154
PciSI GCTCTTC 4 cut(s) 279, 651, 2835, 5849
PcsI WCGNNNNNNNCGW 2 cut(s) 253, 5905
PdmI GAANNNNTTC 4 cut(s) 872, 3522, 4222, 5325
PflMI CCANNNNNTGG 2 cut(s) 1127, 1382
PfoI TCCNGGA 3 cut(s) 2919, 4006, 5915
PinAI ACCGGT 1 cut(s) 1717
PleI GAGTC 5 cut(s) 1101, 1159, 3223, 3553, 5734
PmaCI CACGTG 1 cut(s) 341
PmlI CACGTG 1 cut(s) 341
PpsI GAGTC 5 cut(s) 1101, 1159, 3223, 3553, 5734
Ppu21I YACGTR 2 cut(s) 341, 2099
PpuMI RGGWCCY 1 cut(s) 4010
PscI ACATGT 2 cut(s) 3088, 3154
PshBI ATTAAT 1 cut(s) 5564
PsiI TTATAA 3 cut(s) 1577, 2495, 2756
Psp124BI GAGCTC 1 cut(s) 5067
Psp5II RGGWCCY 1 cut(s) 4010
PspCI CACGTG 1 cut(s) 341
PspEI GGTNACC 3 cut(s) 927, 2798, 5161
PspFI CCCAGC 2 cut(s) 1082, 1853
PspPPI RGGWCCY 1 cut(s) 4010
PstI CTGCAG 2 cut(s) 723, 5628
PstNI CAGNNNCTG 4 cut(s) 1313, 1697, 3728, 4013
PsuI RGATCY 6 cut(s) 229, 2297, 2488, 2589, 4973, 5091
PvuII CAGCTG 2 cut(s) 71, 1694
RruI TCGCGA 1 cut(s) 2688
RsaI GTAC 9 cut(s) 1997, 2097, 3565, 3584, 3969, 4642, 5087, 5376, 5534
RsaNI GTAC 9 cut(s) 1996, 2096, 3564, 3583, 3968, 4641, 5086, 5375, 5533
RseI CAYNNNNRTG 6 cut(s) 3227, 3746, 4329, 4469, 4935, 4959
SacI GAGCTC 1 cut(s) 5067
SapI GCTCTTC 4 cut(s) 279, 651, 2835, 5849
SbfI CCTGCAGG 1 cut(s) 5628
ScaI AGTACT 1 cut(s) 4642
SchI GAGTC 5 cut(s) 1102, 1159, 3224, 3553, 5734
SdaI CCTGCAGG 1 cut(s) 5628
SduI GDGCHC 9 cut(s) 424, 771, 798, 850, 1979, 2176, 2830, 3802, 5067
SexAI ACCWGGT 2 cut(s) 3981, 4692
SfcI CTRYAG 5 cut(s) 719, 1906, 4977, 5023, 5624
SinI GGWCC 4 cut(s) 2072, 2551, 2737, 4010
SmiMI CAYNNNNRTG 6 cut(s) 3227, 3746, 4329, 4469, 4935, 4959
SmlI CTYRAG 4 cut(s) 1892, 3461, 4157, 4728
SmoI CTYRAG 4 cut(s) 1892, 3461, 4157, 4728
SnaBI TACGTA 1 cut(s) 2099
Sse8387I CCTGCAGG 1 cut(s) 5628
SspI AATATT 4 cut(s) 1536, 1590, 2235, 5568
SstI GAGCTC 1 cut(s) 5067
StyI CCWWGG 6 cut(s) 432, 668, 2266, 2613, 3222, 4383
TaiI ACGT 8 cut(s) 250, 286, 343, 1182, 1345, 2101, 3231, 5347
TaqII GACCGA 1 cut(s) 4481
TatI WGTACW 4 cut(s) 1995, 3582, 3967, 4640
TauI GCSGC 5 cut(s) 37, 40, 43, 49, 92
TscAI CASTG 8 cut(s) 106, 1238, 1278, 2120, 4657, 4668, 5143, 5530
TseFI GTSAC 8 cut(s) 1162, 1836, 1935, 2023, 2798, 2938, 4267, 5593
Tsp45I GTSAC 8 cut(s) 1162, 1836, 1935, 2023, 2798, 2938, 4267, 5593
TspGWI ACGGA 7 cut(s) 2249, 2293, 2798, 3009, 4424, 4586, 4957
TspRI CASTG 8 cut(s) 106, 1238, 1278, 2120, 4657, 4668, 5143, 5530
Van91I CCANNNNNTGG 2 cut(s) 1127, 1382
Vha464I CTTAAG 1 cut(s) 4157
VneI GTGCAC 3 cut(s) 420, 794, 3798
VpaK11BI GGWCC 4 cut(s) 2072, 2551, 2737, 4010
VspI ATTAAT 1 cut(s) 5564
XagI CCTNNNNNAGG 1 cut(s) 83
XbaI TCTAGA 1 cut(s) 1612
XceI RCATGY 5 cut(s) 1462, 2674, 2929, 3092, 3158
XcmI CCANNNNNNNNNTGG 3 cut(s) 145, 3379, 5440
XmiI GTMKAC 1 cut(s) 1733
XmnI GAANNNNTTC 4 cut(s) 872, 3522, 4222, 5325
ZrmI AGTACT 1 cut(s) 4642
Zsp2I ATGCAT 2 cut(s) 1971, 2676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.