Rmu_sc0002399.1_g000008

Iron-sulfur cluster co-chaperone protein HscB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002399.1
OG0004741
Physical Location & Seq
Reverse (-)
28904 .. 32884
3981 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1005 bp
atgtcgaagacgaagctactgactcctctctcctctcttctcctcctccgacgaaccctaacttccacccctcatttctcaatcccacacttgcaatcccgcaccaatttctcccccaatatcgaaaagctccgcccccttttcccgctccccggatttgattttcccggcaaatctttcgtctcctcccaatttggggacaaacccaattccgcccactgctggaactgcagcgctgccgctcgaacgacgccgtttctgctctgctcctcctgtcggtgcatccaaccccttgatcactccgtcgattactttcagatatttggactggagaggaagtatgatagtgtggaggaacatctggagggaaaatataaggattggcagaaaaagttgcaccctgatcttgttcattcaaaatcagagaaggagagagagtacgctgctgaacagtctgctcgcgtgattgatgcataccgcacgcttagcaagcccttggcgagggcaatatatattctgaagcttgaaggtgtagacatcgatgaagaagagacactttcagacttagatttgttaagtgagatgcaagagaaactgaagcagtggtctaattcttttgccgaggcatttgaaagccggaactttgaagaagctgtaaaagctatccggcgaatgacttactatgagcgttgcatgagccttccatcttctctacttcacagttcacaatcattgtactcctctcaggttgccaccttttcttttctgcttatggcgtctcaaccaccttctccgtctgatcaccccaaatcacctccttctcctaatgcccctcccaacttgcatcctataaccggaaccctttggggaaggttcaagtcatcatgctccatcatctcctccagattatcatcaaacaaatcacaatcagtctctattcatggcccatcggggacattattctattccatttattgctcctccaccagtggtggaacttcttga

Protein Analysis

334

Amino Acids

37.52

Weight (kDa)

8.53

Isoelectric Point (pI)

59.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSCB_C PF07743 190 - 230 7.7e-06 HSCB C-terminal oligomerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 148, 242
AccI GTMKAC 1 cut(s) 534
AccII CGCG 1 cut(s) 462
AciI CCGC 6 cut(s) 100, 133, 146, 213, 240, 478
AcuI CTGAAG 2 cut(s) 539, 617
AcyI GRCGYC 2 cut(s) 251, 776
AfaI GTAC 2 cut(s) 440, 737
AfeI AGCGCT 1 cut(s) 235
AfiI CCNNNNNNNGG 7 cut(s) 152, 195, 196, 222, 276, 501, 854
AgsI TTSAA 5 cut(s) 417, 527, 632, 647, 877
AluBI AGCT 5 cut(s) 16, 130, 523, 653, 662
AluI AGCT 5 cut(s) 16, 130, 523, 653, 662
Alw26I GTCTC 4 cut(s) 187, 545, 783, 937
Aor51HI AGCGCT 1 cut(s) 235
AoxI GGCC 1 cut(s) 943
ApeKI GCWGC 3 cut(s) 231, 236, 443
ArsI GACNNNNNNTTYG 2 cut(s) 165, 197
AspLEI GCGC 1 cut(s) 236
AspS9I GGNCC 1 cut(s) 944
AsuC2I CCSGG 2 cut(s) 153, 168
AsuHPI GGTGA 2 cut(s) 794, 804
BbsI GAAGAC 1 cut(s) 14
BbvI GCAGC 3 cut(s) 223, 243, 430
BccI CCATC 3 cut(s) 712, 899, 955
BceAI ACGGC 1 cut(s) 238
BcgI CGANNNNNNTGC 2 cut(s) 160, 194
BclI TGATCA 2 cut(s) 295, 799
BcnI CCSGG 2 cut(s) 153, 168
BcoDI GTCTC 4 cut(s) 187, 545, 783, 937
BfmI CTRYAG 1 cut(s) 229
BfoI RGCGCY 1 cut(s) 237
BisI GCNGC 4 cut(s) 232, 237, 240, 444
BlpI GCTNAGC 1 cut(s) 485
BlsI GCNGC 4 cut(s) 233, 238, 241, 445
Bme1390I CCNGG 2 cut(s) 153, 168
BmgT120I GGNCC 1 cut(s) 944
BmiI GGNNCC 1 cut(s) 859
BmrFI CCNGG 2 cut(s) 153, 168
BmsI GCATC 4 cut(s) 291, 460, 573, 853
BpiI GAAGAC 1 cut(s) 14
BpmI CTGGAG 3 cut(s) 350, 383, 886
Bpu1102I GCTNAGC 1 cut(s) 485
BpuMI CCSGG 2 cut(s) 153, 168
Bsa29I ATCGAT 1 cut(s) 540
BsaBI GATNNNNATC 1 cut(s) 910
BsaHI GRCGYC 2 cut(s) 251, 776
BsaJI CCNNGG 3 cut(s) 151, 495, 621
BsaWI WCCGGW 1 cut(s) 854
BsaXI ACNNNNNCTCC 4 cut(s) 323, 353, 422, 452
Bsc4I CCNNNNNNNGG 7 cut(s) 152, 195, 196, 222, 276, 501, 854
Bse1I ACTGG 2 cut(s) 333, 987
Bse8I GATNNNNATC 1 cut(s) 910
BseCI ATCGAT 1 cut(s) 540
BseDI CCNNGG 3 cut(s) 151, 495, 621
BseGI GGATG 2 cut(s) 282, 844
BseJI GATNNNNATC 1 cut(s) 910
BseLI CCNNNNNNNGG 7 cut(s) 152, 195, 196, 222, 276, 501, 854
BseMII CTCAG 1 cut(s) 758
BseNI ACTGG 2 cut(s) 333, 987
BseRI GAGGAG 9 cut(s) 15, 22, 32, 35, 175, 259, 730, 889, 970
BseXI GCAGC 3 cut(s) 223, 243, 430
Bsh1236I CGCG 1 cut(s) 462
BshFI GGCC 1 cut(s) 945
BshVI ATCGAT 1 cut(s) 540
BsiSI CCGG 5 cut(s) 153, 168, 637, 667, 855
BslFI GGGAC 2 cut(s) 212, 967
BslI CCNNNNNNNGG 7 cut(s) 152, 195, 196, 222, 276, 501, 854
BsmAI GTCTC 4 cut(s) 187, 545, 783, 937
BsmBI CGTCTC 2 cut(s) 187, 783
BsmFI GGGAC 2 cut(s) 212, 967
BsnI GGCC 1 cut(s) 945
Bsp143I GATC 3 cut(s) 295, 403, 799
Bsp1720I GCTNAGC 1 cut(s) 485
BspACI CCGC 6 cut(s) 100, 133, 146, 213, 240, 478
BspANI GGCC 1 cut(s) 945
BspCNI CTCAG 1 cut(s) 757
BspDI ATCGAT 1 cut(s) 540
BspFNI CGCG 1 cut(s) 462
BspLI GGNNCC 1 cut(s) 859
BspMAI CTGCAG 1 cut(s) 233
BsrBI CCGCTC 2 cut(s) 148, 242
BsrI ACTGG 2 cut(s) 333, 987
BssECI CCNNGG 3 cut(s) 151, 495, 621
BssMI GATC 3 cut(s) 295, 403, 799
BssNI GRCGYC 2 cut(s) 251, 776
BssT1I CCWWGG 1 cut(s) 495
Bst4CI ACNGT 2 cut(s) 453, 722
Bst6I CTCTTC 2 cut(s) 42, 543
BstACI GRCGYC 2 cut(s) 251, 776
BstC8I GCNNGC 3 cut(s) 460, 482, 491
BstDEI CTNAG 3 cut(s) 485, 565, 744
BstENI CCTNNNNNAGG 1 cut(s) 499
BstF5I GGATG 2 cut(s) 282, 844
BstFNI CGCG 1 cut(s) 462
BstH2I RGCGCY 1 cut(s) 237
BstHHI GCGC 1 cut(s) 236
BstKTI GATC 3 cut(s) 298, 406, 802
BstMAI GTCTC 4 cut(s) 187, 545, 783, 937
BstMBI GATC 3 cut(s) 295, 403, 799
BstMWI GCNNNNNNNGC 5 cut(s) 228, 259, 486, 490, 659
BstSCI CCNGG 2 cut(s) 151, 166
BstSFI CTRYAG 1 cut(s) 229
BstUI CGCG 1 cut(s) 462
BstV1I GCAGC 3 cut(s) 223, 243, 430
BstV2I GAAGAC 1 cut(s) 14
Bsu15I ATCGAT 1 cut(s) 540
BsuRI GGCC 1 cut(s) 945
BsuTUI ATCGAT 1 cut(s) 540
BtsCI GGATG 2 cut(s) 282, 844
BtsI GCAGTG 2 cut(s) 217, 608
BtsIMutI CAGTG 3 cut(s) 217, 608, 994
Cac8I GCNNGC 3 cut(s) 460, 482, 491
CfoI GCGC 1 cut(s) 236
Cfr13I GGNCC 1 cut(s) 944
ClaI ATCGAT 1 cut(s) 540
CseI GACGC 2 cut(s) 259, 765
Csp6I GTAC 2 cut(s) 439, 736
CviAII CATG 3 cut(s) 694, 885, 941
CviJI RGCY 9 cut(s) 16, 130, 493, 523, 636, 653, 662, 699, 945
CviKI_1 RGCY 9 cut(s) 16, 130, 493, 523, 636, 653, 662, 699, 945
CviQI GTAC 2 cut(s) 439, 736
DdeI CTNAG 3 cut(s) 485, 565, 744
DpnI GATC 3 cut(s) 297, 405, 801
DpnII GATC 3 cut(s) 295, 403, 799
Eam1104I CTCTTC 2 cut(s) 42, 543
EarI CTCTTC 2 cut(s) 42, 543
EciI GGCGGA 2 cut(s) 122, 202
Eco130I CCWWGG 1 cut(s) 495
Eco47III AGCGCT 1 cut(s) 235
Eco57I CTGAAG 2 cut(s) 539, 617
EcoNI CCTNNNNNAGG 1 cut(s) 499
EcoT14I CCWWGG 1 cut(s) 495
EcoT22I ATGCAT 1 cut(s) 475
ErhI CCWWGG 1 cut(s) 495
Esp3I CGTCTC 2 cut(s) 187, 783
FaeI CATG 3 cut(s) 697, 888, 944
FalI AAGNNNNNCTT 2 cut(s) 540, 572
FaqI GGGAC 2 cut(s) 212, 967
FatI CATG 3 cut(s) 693, 884, 940
FauI CCCGC 2 cut(s) 107, 153
FbaI TGATCA 2 cut(s) 295, 799
FblI GTMKAC 1 cut(s) 534
Fnu4HI GCNGC 4 cut(s) 232, 237, 240, 444
FokI GGATG 2 cut(s) 269, 831
Fsp4HI GCNGC 4 cut(s) 232, 237, 240, 444
GlaI GCGC 1 cut(s) 235
GluI GCNGC 4 cut(s) 232, 237, 240, 444
GsuI CTGGAG 3 cut(s) 350, 383, 886
HaeII RGCGCY 1 cut(s) 237
HaeIII GGCC 1 cut(s) 945
HapII CCGG 5 cut(s) 153, 168, 637, 667, 855
HgaI GACGC 2 cut(s) 259, 765
HhaI GCGC 1 cut(s) 236
Hin1I GRCGYC 2 cut(s) 251, 776
Hin1II CATG 3 cut(s) 697, 888, 944
Hin6I GCGC 1 cut(s) 234
HinP1I GCGC 1 cut(s) 234
HindIII AAGCTT 1 cut(s) 521
HinfI GANTC 1 cut(s) 22
HpaII CCGG 5 cut(s) 153, 168, 637, 667, 855
HphI GGTGA 2 cut(s) 794, 804
Hpy166II GTNNAC 2 cut(s) 535, 725
Hpy188I TCNGA 6 cut(s) 50, 318, 424, 519, 562, 799
Hpy188III TCNNGA 3 cut(s) 362, 903, 1002
Hpy8I GTNNAC 2 cut(s) 535, 725
Hpy99I CGWCG 3 cut(s) 54, 253, 308
HpyAV CCTTC 6 cut(s) 421, 521, 710, 798, 828, 864
HpyCH4III ACNGT 2 cut(s) 453, 722
HpyCH4V TGCA 8 cut(s) 94, 231, 282, 397, 473, 586, 693, 844
HpyF10VI GCNNNNNNNGC 5 cut(s) 228, 259, 486, 490, 659
HpyF3I CTNAG 3 cut(s) 485, 565, 744
Hsp92I GRCGYC 2 cut(s) 251, 776
Hsp92II CATG 3 cut(s) 697, 888, 944
HspAI GCGC 1 cut(s) 234
Ksp22I TGATCA 2 cut(s) 295, 799
Kzo9I GATC 3 cut(s) 295, 403, 799
LmnI GCTCC 5 cut(s) 135, 153, 272, 893, 983
Lsp1109I GCAGC 3 cut(s) 223, 243, 430
LweI GCATC 4 cut(s) 291, 460, 573, 853
MalI GATC 3 cut(s) 297, 405, 801
MbiI CCGCTC 2 cut(s) 148, 242
MboI GATC 3 cut(s) 295, 403, 799
MboII GAAGA 6 cut(s) 19, 29, 557, 560, 659, 699
MluCI AATT 4 cut(s) 106, 191, 208, 610
MlyI GAGTC 1 cut(s) 16
MmeI TCCRAC 2 cut(s) 73, 310
Mph1103I ATGCAT 1 cut(s) 475
MseI TTAA 1 cut(s) 575
MspI CCGG 5 cut(s) 153, 168, 637, 667, 855
MspR9I CCNGG 2 cut(s) 153, 168
MvnI CGCG 1 cut(s) 462
MwoI GCNNNNNNNGC 5 cut(s) 228, 259, 486, 490, 659
NciI CCSGG 2 cut(s) 153, 168
NdeII GATC 3 cut(s) 295, 403, 799
NlaIII CATG 3 cut(s) 697, 888, 944
NlaIV GGNNCC 1 cut(s) 859
NmeAIII GCCGAG 1 cut(s) 646
NsiI ATGCAT 1 cut(s) 475
PkrI GCNGC 4 cut(s) 233, 238, 241, 445
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
PspN4I GGNNCC 1 cut(s) 859
PspPI GGNCC 1 cut(s) 944
PstI CTGCAG 1 cut(s) 233
RsaI GTAC 2 cut(s) 440, 737
RsaNI GTAC 2 cut(s) 439, 736
SaqAI TTAA 1 cut(s) 575
SatI GCNGC 4 cut(s) 232, 237, 240, 444
Sau3AI GATC 3 cut(s) 295, 403, 799
Sau96I GGNCC 1 cut(s) 944
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 2 cut(s) 153, 168
SfaNI GCATC 4 cut(s) 291, 460, 573, 853
SfcI CTRYAG 1 cut(s) 229
Sse9I AATT 4 cut(s) 106, 191, 208, 610
SsiI CCGC 6 cut(s) 100, 133, 146, 213, 240, 478
StyD4I CCNGG 2 cut(s) 151, 166
StyI CCWWGG 1 cut(s) 495
TaaI ACNGT 2 cut(s) 453, 722
TaqI TCGA 5 cut(s) 5, 123, 244, 306, 540
TasI AATT 4 cut(s) 106, 191, 208, 610
TatI WGTACW 1 cut(s) 735
TauI GCSGC 1 cut(s) 242
Tru1I TTAA 1 cut(s) 575
Tru9I TTAA 1 cut(s) 575
TscAI CASTG 3 cut(s) 224, 608, 994
TseI GCWGC 3 cut(s) 231, 236, 443
TspDTI ATGAA 3 cut(s) 401, 558, 929
TspGWI ACGGA 2 cut(s) 292, 783
TspRI CASTG 3 cut(s) 224, 608, 994
XagI CCTNNNNNAGG 1 cut(s) 499
XmiI GTMKAC 1 cut(s) 534
Zsp2I ATGCAT 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.