Rmu_sc0002433.1_g000013

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002433.1
OG0005474
Physical Location & Seq
Forward (+)
83675 .. 87854
4180 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 909 bp
atggagaagctatcggtggtggagtcgaagctggcgaagttgcttccaaaggagaagagtccgaataaggaggatccagggtgtgggctgatgttgaagctagactacgacgacgttttgagtgcttggtcagacaaagcgtcgtcgttttcggaggatttgcagggcggcgctgatggaaaccatgtctctatatgttccattacgaacaaagaagataagccagagtcctctgaggatgggtgggcggggaggattctagatcttgatgtccggcttcctctgagcacgctgatggtggcgggtactggatacagatcatttgttcgtcgcagaaagatgaagagccaaagcagttatgcacctccgtcttatattcccttgggccaatcagattcgggatcacctgttgttttacgcaatgaagaccatcccaagcagcaaaataatgtcgatcggccgatacaatggtcttctggaatttgcgcatgttgtgatgatacgcagagctgttgtataggtttattttgtccttgctatctgttcggaaagaatgcagagtttctgggctctgggacattaatgggctcctgcacgactcattttattctgtgggtacttgttaataccgtctgttgcttattaactgatggccttattttgggtataccgggatgctttgttgcatgttatgcttgtggctaccgcagggcactaagatcaaaatataatctccaggaagcaccgtgtggagactttattactcattttttctgccatctgtgtgcaatttgtcaagagtaccgggagatccgtgaaaagtctgctgaatctggccctggtgatctgaatctagccatagttacagccccagcagtccagacaatggaaccagggcttgacaagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

302

Amino Acids

33.02

Weight (kDa)

5.46

Isoelectric Point (pI)

57.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 157 - 270 3.2e-28 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 487
AccB7I CCANNNNNTGG 2 cut(s) 83, 886
AccI GTMKAC 1 cut(s) 667
AciI CCGC 4 cut(s) 168, 248, 302, 706
AclWI GGATC 4 cut(s) 68, 81, 409, 805
AcoI YGGCCR 1 cut(s) 458
AcsI RAATTY 1 cut(s) 480
AdeI CACNNNGTG 1 cut(s) 749
AfaI GTAC 3 cut(s) 307, 618, 803
AfiI CCNNNNNNNGG 3 cut(s) 83, 661, 886
AgsI TTSAA 1 cut(s) 97
AhdI GACNNNNNGTC 1 cut(s) 139
AjnI CCWGG 4 cut(s) 76, 735, 838, 892
AluBI AGCT 4 cut(s) 10, 31, 100, 510
AluI AGCT 4 cut(s) 10, 31, 100, 510
Alw21I GWGCWC 1 cut(s) 290
Alw26I GTCTC 2 cut(s) 193, 747
AlwI GGATC 4 cut(s) 68, 81, 409, 805
AoxI GGCC 4 cut(s) 385, 458, 652, 835
ApeKI GCWGC 1 cut(s) 439
ApoI RAATTY 1 cut(s) 480
AseI ATTAAT 1 cut(s) 581
AspLEI GCGC 2 cut(s) 173, 488
AspS9I GGNCC 2 cut(s) 385, 836
AsuC2I CCSGG 2 cut(s) 672, 806
AsuHPI GGTGA 2 cut(s) 396, 854
BaeGI GKGCMC 1 cut(s) 715
BaeI ACNNNNGTAYC 2 cut(s) 455, 488
BamHI GGATCC 1 cut(s) 73
BanII GRGCYC 2 cut(s) 572, 590
BarI GAAGNNNNNNTAC 2 cut(s) 89, 121
BbsI GAAGAC 2 cut(s) 432, 465
Bbv12I GWGCWC 1 cut(s) 290
BbvI GCAGC 1 cut(s) 451
BccI CCATC 6 cut(s) 170, 233, 289, 438, 644, 786
BciT130I CCWGG 4 cut(s) 78, 737, 840, 894
BciVI GTATCC 1 cut(s) 305
BcnI CCSGG 2 cut(s) 672, 806
BcoDI GTCTC 2 cut(s) 193, 747
BfaI CTAG 3 cut(s) 101, 260, 854
BfoI RGCGCY 1 cut(s) 174
BfuI GTATCC 1 cut(s) 305
BglII AGATCT 1 cut(s) 262
BisI GCNGC 2 cut(s) 169, 440
BlsI GCNGC 2 cut(s) 170, 441
Bme1390I CCNGG 6 cut(s) 78, 672, 737, 806, 840, 894
BmeRI GACNNNNNGTC 1 cut(s) 139
BmgT120I GGNCC 2 cut(s) 385, 836
BmiI GGNNCC 3 cut(s) 75, 589, 891
BmrFI CCNGG 6 cut(s) 78, 672, 737, 806, 840, 894
BmsI GCATC 1 cut(s) 665
BpiI GAAGAC 2 cut(s) 432, 465
BpmI CTGGAG 1 cut(s) 719
BpuMI CCSGG 2 cut(s) 672, 806
BsaBI GATNNNNATC 2 cut(s) 316, 849
BsaJI CCNNGG 4 cut(s) 77, 381, 838, 893
Bsc4I CCNNNNNNNGG 3 cut(s) 83, 661, 886
Bse1I ACTGG 1 cut(s) 313
Bse3DI GCAATG 1 cut(s) 427
Bse8I GATNNNNATC 2 cut(s) 316, 849
BseBI CCWGG 4 cut(s) 78, 737, 840, 894
BseDI CCNNGG 4 cut(s) 77, 381, 838, 893
BseGI GGATG 3 cut(s) 244, 430, 680
BseJI GATNNNNATC 2 cut(s) 316, 849
BseLI CCNNNNNNNGG 3 cut(s) 83, 661, 886
BseMI GCAATG 1 cut(s) 427
BseMII CTCAG 2 cut(s) 225, 275
BseNI ACTGG 1 cut(s) 313
BseSI GKGCMC 1 cut(s) 715
BseX3I CGGCCG 1 cut(s) 458
BseXI GCAGC 1 cut(s) 451
BseYI CCCAGC 1 cut(s) 871
BsgI GTGCAG 1 cut(s) 577
Bsh1285I CGRYCG 2 cut(s) 457, 461
BshFI GGCC 4 cut(s) 387, 460, 654, 837
BsiEI CGRYCG 2 cut(s) 457, 461
BsiHKAI GWGCWC 1 cut(s) 290
BsiSI CCGG 3 cut(s) 274, 671, 805
BslFI GGGAC 1 cut(s) 589
BslI CCNNNNNNNGG 3 cut(s) 83, 661, 886
BsmAI GTCTC 2 cut(s) 193, 747
BsmFI GGGAC 1 cut(s) 589
BsmI GAATGC 1 cut(s) 559
BsnI GGCC 4 cut(s) 387, 460, 654, 837
Bsp1286I GDGCHC 4 cut(s) 290, 572, 590, 715
Bsp143I GATC 8 cut(s) 73, 262, 317, 401, 454, 719, 810, 844
BspACI CCGC 4 cut(s) 168, 248, 302, 706
BspANI GGCC 4 cut(s) 387, 460, 654, 837
BspCNI CTCAG 2 cut(s) 226, 276
BspLI GGNNCC 3 cut(s) 75, 589, 891
BspPI GGATC 4 cut(s) 68, 81, 409, 805
BspQI GCTCTTC 1 cut(s) 338
BsrDI GCAATG 1 cut(s) 427
BsrI ACTGG 1 cut(s) 313
BssECI CCNNGG 4 cut(s) 77, 381, 838, 893
BssMI GATC 8 cut(s) 73, 262, 317, 401, 454, 719, 810, 844
BssNAI GTATAC 1 cut(s) 668
BssT1I CCWWGG 1 cut(s) 381
Bst1107I GTATAC 1 cut(s) 668
Bst2UI CCWGG 4 cut(s) 78, 737, 840, 894
Bst4CI ACNGT 2 cut(s) 631, 747
Bst6I CTCTTC 2 cut(s) 50, 338
BstAPI GCANNNNNTGC 1 cut(s) 692
BstC8I GCNNGC 2 cut(s) 33, 290
BstDEI CTNAG 3 cut(s) 234, 284, 716
BstF5I GGATG 3 cut(s) 244, 430, 680
BstH2I RGCGCY 1 cut(s) 174
BstHHI GCGC 2 cut(s) 173, 488
BstKTI GATC 8 cut(s) 76, 265, 320, 404, 457, 722, 813, 847
BstMAI GTCTC 2 cut(s) 193, 747
BstMBI GATC 8 cut(s) 73, 262, 317, 401, 454, 719, 810, 844
BstMCI CGRYCG 2 cut(s) 457, 461
BstMWI GCNNNNNNNGC 1 cut(s) 692
BstNI CCWGG 4 cut(s) 78, 737, 840, 894
BstNSI RCATGY 2 cut(s) 492, 690
BstSCI CCNGG 6 cut(s) 76, 670, 735, 804, 838, 892
BstSLI GKGCMC 1 cut(s) 715
BstV1I GCAGC 1 cut(s) 451
BstV2I GAAGAC 2 cut(s) 432, 465
BstX2I RGATCY 3 cut(s) 73, 262, 810
BstYI RGATCY 3 cut(s) 73, 262, 810
BstZ17I GTATAC 1 cut(s) 668
BstZI CGGCCG 1 cut(s) 458
BsuI GTATCC 1 cut(s) 305
BsuRI GGCC 4 cut(s) 387, 460, 654, 837
BtsCI GGATG 3 cut(s) 244, 430, 680
Cac8I GCNNGC 2 cut(s) 33, 290
CfoI GCGC 2 cut(s) 173, 488
Cfr13I GGNCC 2 cut(s) 385, 836
CseI GACGC 1 cut(s) 129
Csp6I GTAC 3 cut(s) 306, 617, 802
CviAII CATG 3 cut(s) 185, 489, 687
CviQI GTAC 3 cut(s) 306, 617, 802
DdeI CTNAG 3 cut(s) 234, 284, 716
DpnI GATC 8 cut(s) 75, 264, 319, 403, 456, 721, 812, 846
DpnII GATC 8 cut(s) 73, 262, 317, 401, 454, 719, 810, 844
DraIII CACNNNGTG 1 cut(s) 749
DriI GACNNNNNGTC 1 cut(s) 139
EaeI YGGCCR 1 cut(s) 458
EagI CGGCCG 1 cut(s) 458
Eam1104I CTCTTC 2 cut(s) 50, 338
Eam1105I GACNNNNNGTC 1 cut(s) 139
EarI CTCTTC 2 cut(s) 50, 338
EclXI CGGCCG 1 cut(s) 458
Eco130I CCWWGG 1 cut(s) 381
Eco24I GRGCYC 2 cut(s) 572, 590
Eco52I CGGCCG 1 cut(s) 458
EcoRII CCWGG 4 cut(s) 76, 735, 838, 892
EcoT14I CCWWGG 1 cut(s) 381
EcoT38I GRGCYC 2 cut(s) 572, 590
ErhI CCWWGG 1 cut(s) 381
FaeI CATG 3 cut(s) 188, 492, 690
FaqI GGGAC 1 cut(s) 589
FatI CATG 3 cut(s) 184, 488, 686
FauI CCCGC 2 cut(s) 241, 295
FblI GTMKAC 1 cut(s) 667
Fnu4HI GCNGC 2 cut(s) 169, 440
FokI GGATG 3 cut(s) 251, 417, 687
FriOI GRGCYC 2 cut(s) 572, 590
Fsp4HI GCNGC 2 cut(s) 169, 440
FspBI CTAG 3 cut(s) 101, 260, 854
FspI TGCGCA 1 cut(s) 487
GlaI GCGC 2 cut(s) 172, 487
GluI GCNGC 2 cut(s) 169, 440
GsaI CCCAGC 1 cut(s) 875
GsuI CTGGAG 1 cut(s) 719
HaeII RGCGCY 1 cut(s) 174
HaeIII GGCC 4 cut(s) 387, 460, 654, 837
HapII CCGG 3 cut(s) 274, 671, 805
HgaI GACGC 1 cut(s) 129
HhaI GCGC 2 cut(s) 173, 488
Hin1II CATG 3 cut(s) 188, 492, 690
Hin6I GCGC 2 cut(s) 171, 486
HinP1I GCGC 2 cut(s) 171, 486
HinfI GANTC 8 cut(s) 23, 58, 227, 256, 395, 598, 830, 850
HpaII CCGG 3 cut(s) 274, 671, 805
HphI GGTGA 2 cut(s) 396, 854
Hpy166II GTNNAC 1 cut(s) 668
Hpy188I TCNGA 8 cut(s) 63, 133, 154, 235, 285, 394, 548, 849
Hpy188III TCNNGA 6 cut(s) 260, 266, 399, 477, 797, 880
Hpy8I GTNNAC 1 cut(s) 668
Hpy99I CGWCG 5 cut(s) 113, 116, 145, 148, 333
HpyCH4III ACNGT 2 cut(s) 631, 747
HpyCH4IV ACGT 1 cut(s) 114
HpyCH4V TGCA 6 cut(s) 163, 362, 557, 594, 686, 788
HpyF10VI GCNNNNNNNGC 1 cut(s) 692
HpyF3I CTNAG 3 cut(s) 234, 284, 716
HpySE526I ACGT 1 cut(s) 114
Hsp92II CATG 3 cut(s) 188, 492, 690
HspAI GCGC 2 cut(s) 171, 486
Kzo9I GATC 8 cut(s) 73, 262, 317, 401, 454, 719, 810, 844
LguI GCTCTTC 1 cut(s) 338
LmnI GCTCC 1 cut(s) 593
Lsp1109I GCAGC 1 cut(s) 451
LweI GCATC 1 cut(s) 665
MaeI CTAG 3 cut(s) 101, 260, 854
MaeII ACGT 1 cut(s) 114
MaeIII GTNAC 1 cut(s) 862
MalI GATC 8 cut(s) 75, 264, 319, 403, 456, 721, 812, 846
MboI GATC 8 cut(s) 73, 262, 317, 401, 454, 719, 810, 844
MboII GAAGA 5 cut(s) 67, 227, 355, 437, 465
MflI RGATCY 3 cut(s) 73, 262, 810
MhlI GDGCHC 4 cut(s) 290, 572, 590, 715
MluCI AATT 2 cut(s) 480, 789
MlyI GAGTC 4 cut(s) 32, 67, 236, 592
MnlI CCTC 7 cut(s) 64, 148, 229, 241, 246, 291, 375
MseI TTAA 3 cut(s) 581, 624, 644
MslI CAYNNNNRTG 2 cut(s) 293, 783
MspI CCGG 3 cut(s) 274, 671, 805
MspR9I CCNGG 6 cut(s) 78, 672, 737, 806, 840, 894
Mva1269I GAATGC 1 cut(s) 559
MvaI CCWGG 4 cut(s) 78, 737, 840, 894
MwoI GCNNNNNNNGC 1 cut(s) 692
NciI CCSGG 2 cut(s) 672, 806
NdeII GATC 8 cut(s) 73, 262, 317, 401, 454, 719, 810, 844
NlaIII CATG 3 cut(s) 188, 492, 690
NlaIV GGNNCC 3 cut(s) 75, 589, 891
NsbI TGCGCA 1 cut(s) 487
NspI RCATGY 2 cut(s) 492, 690
PciSI GCTCTTC 1 cut(s) 338
PcsI WCGNNNNNNNCGW 1 cut(s) 32
PctI GAATGC 1 cut(s) 559
PfeI GAWTC 4 cut(s) 256, 395, 830, 850
PflMI CCANNNNNTGG 2 cut(s) 83, 886
PfoI TCCNGGA 1 cut(s) 735
PkrI GCNGC 2 cut(s) 170, 441
Ple19I CGATCG 1 cut(s) 457
PleI GAGTC 4 cut(s) 31, 66, 235, 592
PpsI GAGTC 4 cut(s) 31, 66, 235, 592
PshBI ATTAAT 1 cut(s) 581
Psp6I CCWGG 4 cut(s) 76, 735, 838, 892
PspFI CCCAGC 1 cut(s) 871
PspGI CCWGG 4 cut(s) 76, 735, 838, 892
PspN4I GGNNCC 3 cut(s) 75, 589, 891
PspPI GGNCC 2 cut(s) 385, 836
PsuI RGATCY 3 cut(s) 73, 262, 810
PvuI CGATCG 1 cut(s) 457
RsaI GTAC 3 cut(s) 307, 618, 803
RsaNI GTAC 3 cut(s) 306, 617, 802
RseI CAYNNNNRTG 2 cut(s) 293, 783
SapI GCTCTTC 1 cut(s) 338
SaqAI TTAA 3 cut(s) 581, 624, 644
SatI GCNGC 2 cut(s) 169, 440
Sau3AI GATC 8 cut(s) 73, 262, 317, 401, 454, 719, 810, 844
Sau96I GGNCC 2 cut(s) 385, 836
SchI GAGTC 4 cut(s) 32, 67, 236, 592
ScrFI CCNGG 6 cut(s) 78, 672, 737, 806, 840, 894
SduI GDGCHC 4 cut(s) 290, 572, 590, 715
SetI ASST 8 cut(s) 12, 33, 102, 117, 367, 409, 512, 523
SfaNI GCATC 1 cut(s) 665
SmiMI CAYNNNNRTG 2 cut(s) 293, 783
Sse9I AATT 2 cut(s) 480, 789
SsiI CCGC 4 cut(s) 168, 248, 302, 706
SspMI CTAG 3 cut(s) 101, 260, 854
StyD4I CCNGG 6 cut(s) 76, 670, 735, 804, 838, 892
StyI CCWWGG 1 cut(s) 381
TaaI ACNGT 2 cut(s) 631, 747
TaiI ACGT 1 cut(s) 117
TaqI TCGA 2 cut(s) 26, 453
TasI AATT 2 cut(s) 480, 789
TauI GCSGC 1 cut(s) 171
TfiI GAWTC 4 cut(s) 256, 395, 830, 850
Tru1I TTAA 3 cut(s) 581, 624, 644
Tru9I TTAA 3 cut(s) 581, 624, 644
TseI GCWGC 1 cut(s) 439
TspDTI ATGAA 2 cut(s) 356, 438
TspGWI ACGGA 2 cut(s) 357, 803
Van91I CCANNNNNTGG 2 cut(s) 83, 886
VspI ATTAAT 1 cut(s) 581
XapI RAATTY 1 cut(s) 480
XbaI TCTAGA 1 cut(s) 259
XceI RCATGY 2 cut(s) 492, 690
XmiI GTMKAC 1 cut(s) 667
XspI CTAG 3 cut(s) 101, 260, 854
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.