Rmu_sc0002700.1_g000004

Belongs to the phosphoglycerate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002700.1
OG0004015
Physical Location & Seq
Forward (+)
28459 .. 33010
4552 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1287 bp
atggttctttcctctcaagcgccaccgaaggcgggggttgatctcaattttcctttggatgacgactgtaagatcacggatgacactagagtccgagcagctgtccccaccatcaagtacttgctcggaaatggtgccagagtcatcctcgcttcccacttgggatgtcccaagggtgtcactcccaagtacagtttgaagcctcttgtgccaagactgtctgaacttcttggcattcaggtcgtgaaggctgaagactttattggtccagaagtagaaaagctggcggcttcacttcctgatggtgctgtccttcttcttgaaaatgtgaggttttacaaagaggaggagaagaccgatcctgagcatgcaaagaagctcgcctctgtagctgatctttttgtcaatgatgcattcggaactgcacatagaacccatgcttcaactgagggtgtaacaaaattcttaaggccatctgtagctggttttcttttgcagaaggaactcgactatcttgttggggtagtatcaaacccaaaaaagccatttgcagccattgttggtggtccgaaggtctcatctaagattggagtgatcgagtcactgttagagatggttaattacttaattcttggtggaggaatgatcttcacattttacaaggcacagggtatctcagtgggttcatctctggtggaaaaggataagctagaactcgctacatcattcattgcaaaggccaaggcaaagggagtgtctcttttgctacccactgatgtcataattacagacaaatttgctcctgatgcaaacagcaagattgttccagcttcaagcatccctgatgggtggatgggattggatattggaccacaccctattaagacattcaatgatgcacttgataccactcaaaccatcatttggaacggaccaataggagtgtttgagtttgacaagtttgcagtaggaacagagcggtggtgttgtccgcacggttgtgtacatggtgacatgcatattctagacattgctgtggatttgccttctgtgactgaaattttcgggtcaaaatattcaactactcttcttctgcactcgattaagatgtcagcccagccaggtgatgaaatcctcgtagcgaggggtggacaaagagggattagcttggtagaaatgccagagcgtagcaagaaaaagttgatggctttgaccactaatgtgatgagagatgatagtgataaggcaaagtggtcatgctttttctcacttggtattccgatgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002682 GO:0002697 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006464 GO:0006468 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009941 GO:0009987 GO:0010035 GO:0010038 GO:0010319 GO:0015977 GO:0015979 GO:0016020 GO:0016051 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019253 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019538 GO:0019637 GO:0019685 GO:0019693 GO:0019752 GO:0030312 GO:0031347 GO:0031967 GO:0031975 GO:0032101 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0036211 GO:0042221 GO:0042866 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043900 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044267 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046939 GO:0048046 GO:0048583 GO:0050688 GO:0050691 GO:0050789 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0065007 GO:0071704 GO:0071944 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0080134 GO:0090407 GO:0140096 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PGK

Protein Analysis

428

Amino Acids

46.2

Weight (kDa)

6.52

Isoelectric Point (pI)

30.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGK PF00162 13 - 327 7.2e-124 Phosphoglycerate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 89
AccB1I GGYRCC 1 cut(s) 134
AccB7I CCANNNNNTGG 1 cut(s) 924
AccBSI CCGCTC 1 cut(s) 979
AciI CCGC 4 cut(s) 32, 287, 979, 992
AclWI GGATC 1 cut(s) 353
AcsI RAATTY 3 cut(s) 461, 794, 1059
AcuI CTGAAG 1 cut(s) 273
AfaI GTAC 3 cut(s) 119, 191, 1005
AfiI CCNNNNNNNGG 2 cut(s) 32, 924
AflII CTTAAG 1 cut(s) 466
AgsI TTSAA 6 cut(s) 199, 323, 444, 834, 892, 1080
AjnI CCWGG 1 cut(s) 1120
AjuI GAANNNNNNNTTGG 2 cut(s) 246, 278
AleI CACNNNNGTG 2 cut(s) 999, 1220
AluBI AGCT 8 cut(s) 101, 283, 379, 392, 482, 709, 830, 1167
AluI AGCT 8 cut(s) 101, 283, 379, 392, 482, 709, 830, 1167
Alw26I GTCTC 2 cut(s) 580, 762
AlwI GGATC 1 cut(s) 353
AoxI GGCC 2 cut(s) 470, 738
ApeKI GCWGC 2 cut(s) 98, 551
ApoI RAATTY 3 cut(s) 461, 794, 1059
ArsI GACNNNNNNTTYG 2 cut(s) 740, 772
AspLEI GCGC 1 cut(s) 22
AspS9I GGNCC 4 cut(s) 266, 566, 869, 932
AsuHPI GGTGA 2 cut(s) 1022, 1136
AvaII GGWCC 4 cut(s) 266, 566, 869, 932
BanI GGYRCC 1 cut(s) 134
BbsI GAAGAC 2 cut(s) 261, 359
BbvI GCAGC 2 cut(s) 110, 563
BccI CCATC 8 cut(s) 119, 296, 481, 607, 839, 847, 926, 1198
BcgI CGANNNNNNTGC 2 cut(s) 223, 257
BciT130I CCWGG 1 cut(s) 1122
BcoDI GTCTC 2 cut(s) 580, 762
BfaI CTAG 3 cut(s) 87, 710, 1025
BfmI CTRYAG 2 cut(s) 387, 477
BfoI RGCGCY 1 cut(s) 23
BfrI CTTAAG 1 cut(s) 466
BisI GCNGC 3 cut(s) 99, 288, 552
BlsI GCNGC 3 cut(s) 100, 289, 553
BmcAI AGTACT 1 cut(s) 119
Bme1390I CCNGG 1 cut(s) 1122
Bme18I GGWCC 4 cut(s) 266, 566, 869, 932
BmgT120I GGNCC 4 cut(s) 266, 566, 869, 932
BmiI GGNNCC 1 cut(s) 136
BmrFI CCNGG 1 cut(s) 1122
BmsI GCATC 4 cut(s) 400, 796, 846, 886
BpiI GAAGAC 2 cut(s) 261, 359
BplI GAGNNNNNCTC 2 cut(s) 132, 164
Bpu10I CCTNAGC 1 cut(s) 363
BsaI GGTCTC 1 cut(s) 580
BsaJI CCNNGG 2 cut(s) 171, 741
Bsc4I CCNNNNNNNGG 2 cut(s) 32, 924
Bse3DI GCAATG 2 cut(s) 729, 1029
BseBI CCWGG 1 cut(s) 1122
BseDI CCNNGG 2 cut(s) 171, 741
BseGI GGATG 6 cut(s) 64, 85, 144, 170, 837, 858
BseLI CCNNNNNNNGG 2 cut(s) 32, 924
BseMI GCAATG 2 cut(s) 729, 1029
BseMII CTCAG 3 cut(s) 354, 438, 690
BseRI GAGGAG 2 cut(s) 359, 362
BseXI GCAGC 2 cut(s) 110, 563
BseYI CCCAGC 1 cut(s) 1116
BsgI GTGCAG 2 cut(s) 408, 1079
BshFI GGCC 2 cut(s) 472, 740
BshNI GGYRCC 1 cut(s) 134
BslFI GGGAC 2 cut(s) 89, 153
BslI CCNNNNNNNGG 2 cut(s) 32, 924
BsmAI GTCTC 2 cut(s) 580, 762
BsmFI GGGAC 2 cut(s) 89, 153
BsmI GAATGC 2 cut(s) 234, 413
BsnI GGCC 2 cut(s) 472, 740
Bso31I GGTCTC 1 cut(s) 580
Bsp1407I TGTACA 1 cut(s) 1003
Bsp143I GATC 6 cut(s) 40, 72, 358, 394, 594, 645
BspACI CCGC 4 cut(s) 32, 287, 979, 992
BspANI GGCC 2 cut(s) 472, 740
BspCNI CTCAG 3 cut(s) 355, 439, 689
BspLI GGNNCC 1 cut(s) 136
BspPI GGATC 1 cut(s) 353
BspT107I GGYRCC 1 cut(s) 134
BspTI CTTAAG 1 cut(s) 466
BspTNI GGTCTC 1 cut(s) 580
BsrBI CCGCTC 1 cut(s) 979
BsrDI GCAATG 2 cut(s) 729, 1029
BsrGI TGTACA 1 cut(s) 1003
BssECI CCNNGG 2 cut(s) 171, 741
BssMI GATC 6 cut(s) 40, 72, 358, 394, 594, 645
BssT1I CCWWGG 2 cut(s) 171, 741
Bst2UI CCWGG 1 cut(s) 1122
Bst4CI ACNGT 5 cut(s) 68, 194, 219, 606, 998
Bst6I CTCTTC 1 cut(s) 1092
BstAFI CTTAAG 1 cut(s) 466
BstAUI TGTACA 1 cut(s) 1003
BstC8I GCNNGC 3 cut(s) 285, 369, 381
BstDEI CTNAG 4 cut(s) 363, 447, 582, 676
BstF5I GGATG 6 cut(s) 64, 85, 144, 170, 837, 858
BstH2I RGCGCY 1 cut(s) 23
BstHHI GCGC 1 cut(s) 22
BstKTI GATC 6 cut(s) 43, 75, 361, 397, 597, 648
BstMAI GTCTC 2 cut(s) 580, 762
BstMBI GATC 6 cut(s) 40, 72, 358, 394, 594, 645
BstMWI GCNNNNNNNGC 3 cut(s) 208, 389, 806
BstNI CCWGG 1 cut(s) 1122
BstNSI RCATGY 2 cut(s) 371, 1018
BstSCI CCNGG 1 cut(s) 1120
BstSFI CTRYAG 2 cut(s) 387, 477
BstV1I GCAGC 2 cut(s) 110, 563
BstV2I GAAGAC 2 cut(s) 261, 359
BsuRI GGCC 2 cut(s) 472, 740
BtsCI GGATG 6 cut(s) 64, 85, 144, 170, 837, 858
BtsIMutI CAGTG 3 cut(s) 602, 684, 771
Cac8I GCNNGC 3 cut(s) 285, 369, 381
CfoI GCGC 1 cut(s) 22
Cfr13I GGNCC 4 cut(s) 266, 566, 869, 932
Csp6I GTAC 3 cut(s) 118, 190, 1004
CviAII CATG 5 cut(s) 368, 437, 1007, 1015, 1257
CviQI GTAC 3 cut(s) 118, 190, 1004
DdeI CTNAG 4 cut(s) 363, 447, 582, 676
DpnI GATC 6 cut(s) 42, 74, 360, 396, 596, 647
DpnII GATC 6 cut(s) 40, 72, 358, 394, 594, 645
DrdI GACNNNNNNGTC 1 cut(s) 89
DseDI GACNNNNNNGTC 1 cut(s) 89
Eam1104I CTCTTC 1 cut(s) 1092
EarI CTCTTC 1 cut(s) 1092
Eco130I CCWWGG 2 cut(s) 171, 741
Eco31I GGTCTC 1 cut(s) 580
Eco47I GGWCC 4 cut(s) 266, 566, 869, 932
Eco57I CTGAAG 1 cut(s) 273
EcoRII CCWGG 1 cut(s) 1120
EcoT14I CCWWGG 2 cut(s) 171, 741
EcoT22I ATGCAT 2 cut(s) 415, 1020
ErhI CCWWGG 2 cut(s) 171, 741
FaeI CATG 5 cut(s) 371, 440, 1010, 1018, 1260
FaiI YATR 8 cut(s) 369, 429, 438, 782, 1008, 1016, 1020, 1258
FaqI GGGAC 2 cut(s) 89, 153
FatI CATG 5 cut(s) 367, 436, 1006, 1014, 1256
FauI CCCGC 1 cut(s) 25
Fnu4HI GCNGC 3 cut(s) 99, 288, 552
FokI GGATG 6 cut(s) 71, 92, 131, 177, 824, 865
Fsp4HI GCNGC 3 cut(s) 99, 288, 552
FspBI CTAG 3 cut(s) 87, 710, 1025
GlaI GCGC 1 cut(s) 21
GluI GCNGC 3 cut(s) 99, 288, 552
GsaI CCCAGC 1 cut(s) 1120
HaeII RGCGCY 1 cut(s) 23
HaeIII GGCC 2 cut(s) 472, 740
HhaI GCGC 1 cut(s) 22
Hin1II CATG 5 cut(s) 371, 440, 1010, 1018, 1260
Hin6I GCGC 1 cut(s) 20
HinP1I GCGC 1 cut(s) 20
HinfI GANTC 3 cut(s) 90, 141, 599
HphI GGTGA 2 cut(s) 1022, 1136
Hpy166II GTNNAC 2 cut(s) 1004, 1151
Hpy188I TCNGA 6 cut(s) 95, 128, 223, 419, 570, 1281
Hpy188III TCNNGA 7 cut(s) 244, 269, 299, 320, 362, 803, 1025
Hpy8I GTNNAC 2 cut(s) 1004, 1151
HpyAV CCTTC 6 cut(s) 22, 241, 323, 493, 565, 1056
HpyCH4III ACNGT 5 cut(s) 68, 194, 219, 606, 998
HpyF10VI GCNNNNNNNGC 3 cut(s) 208, 389, 806
HpyF3I CTNAG 4 cut(s) 363, 447, 582, 676
Hsp92II CATG 5 cut(s) 371, 440, 1010, 1018, 1260
HspAI GCGC 1 cut(s) 20
Kzo9I GATC 6 cut(s) 40, 72, 358, 394, 594, 645
LmnI GCTCC 1 cut(s) 805
Lsp1109I GCAGC 2 cut(s) 110, 563
LweI GCATC 4 cut(s) 400, 796, 846, 886
MaeI CTAG 3 cut(s) 87, 710, 1025
MaeIII GTNAC 5 cut(s) 178, 454, 600, 1010, 1051
MalI GATC 6 cut(s) 42, 74, 360, 396, 596, 647
MbiI CCGCTC 1 cut(s) 979
MboI GATC 6 cut(s) 40, 72, 358, 394, 594, 645
MboII GAAGA 6 cut(s) 266, 308, 364, 640, 1079, 1082
MluCI AATT 7 cut(s) 46, 461, 619, 627, 783, 794, 1059
MlyI GAGTC 3 cut(s) 99, 150, 608
Mph1103I ATGCAT 2 cut(s) 415, 1020
MseI TTAA 5 cut(s) 467, 618, 626, 882, 1104
MslI CAYNNNNRTG 3 cut(s) 999, 1034, 1220
MspA1I CMGCKG 1 cut(s) 101
MspCI CTTAAG 1 cut(s) 466
MspR9I CCNGG 1 cut(s) 1122
Mva1269I GAATGC 2 cut(s) 234, 413
MvaI CCWGG 1 cut(s) 1122
MwoI GCNNNNNNNGC 3 cut(s) 208, 389, 806
NdeII GATC 6 cut(s) 40, 72, 358, 394, 594, 645
NlaIII CATG 5 cut(s) 371, 440, 1010, 1018, 1260
NlaIV GGNNCC 1 cut(s) 136
NmuCI GTSAC 4 cut(s) 178, 600, 1010, 1051
NsiI ATGCAT 2 cut(s) 415, 1020
NspI RCATGY 2 cut(s) 371, 1018
OliI CACNNNNGTG 2 cut(s) 999, 1220
PaeI GCATGC 1 cut(s) 371
PctI GAATGC 2 cut(s) 234, 413
PflMI CCANNNNNTGG 1 cut(s) 924
PkrI GCNGC 3 cut(s) 100, 289, 553
PleI GAGTC 3 cut(s) 98, 149, 607
PpsI GAGTC 3 cut(s) 98, 149, 607
Psp6I CCWGG 1 cut(s) 1120
PspFI CCCAGC 1 cut(s) 1116
PspGI CCWGG 1 cut(s) 1120
PspN4I GGNNCC 1 cut(s) 136
PspPI GGNCC 4 cut(s) 266, 566, 869, 932
PvuII CAGCTG 1 cut(s) 101
RsaI GTAC 3 cut(s) 119, 191, 1005
RsaNI GTAC 3 cut(s) 118, 190, 1004
RseI CAYNNNNRTG 3 cut(s) 999, 1034, 1220
SaqAI TTAA 5 cut(s) 467, 618, 626, 882, 1104
SatI GCNGC 3 cut(s) 99, 288, 552
Sau3AI GATC 6 cut(s) 40, 72, 358, 394, 594, 645
Sau96I GGNCC 4 cut(s) 266, 566, 869, 932
ScaI AGTACT 1 cut(s) 119
SchI GAGTC 3 cut(s) 99, 150, 608
ScrFI CCNGG 1 cut(s) 1122
SfaNI GCATC 4 cut(s) 400, 796, 846, 886
SfcI CTRYAG 2 cut(s) 387, 477
SinI GGWCC 4 cut(s) 266, 566, 869, 932
SmiMI CAYNNNNRTG 3 cut(s) 999, 1034, 1220
SmlI CTYRAG 2 cut(s) 15, 466
SmoI CTYRAG 2 cut(s) 15, 466
SphI GCATGC 1 cut(s) 371
Sse9I AATT 7 cut(s) 46, 461, 619, 627, 783, 794, 1059
SsiI CCGC 4 cut(s) 32, 287, 979, 992
SspI AATATT 1 cut(s) 1076
SspMI CTAG 3 cut(s) 87, 710, 1025
StyD4I CCNGG 1 cut(s) 1120
StyI CCWWGG 2 cut(s) 171, 741
TaaI ACNGT 5 cut(s) 68, 194, 219, 606, 998
TaqI TCGA 3 cut(s) 507, 597, 1100
TaqII GACCGA 1 cut(s) 371
TasI AATT 7 cut(s) 46, 461, 619, 627, 783, 794, 1059
TatI WGTACW 3 cut(s) 117, 189, 1003
TauI GCSGC 1 cut(s) 290
Tru1I TTAA 5 cut(s) 467, 618, 626, 882, 1104
Tru9I TTAA 5 cut(s) 467, 618, 626, 882, 1104
TscAI CASTG 3 cut(s) 609, 684, 778
TseFI GTSAC 4 cut(s) 178, 600, 1010, 1051
TseI GCWGC 2 cut(s) 98, 551
Tsp45I GTSAC 4 cut(s) 178, 600, 1010, 1051
TspDTI ATGAA 3 cut(s) 675, 718, 1143
TspGWI ACGGA 2 cut(s) 92, 945
TspRI CASTG 3 cut(s) 609, 684, 778
Van91I CCANNNNNTGG 1 cut(s) 924
Vha464I CTTAAG 1 cut(s) 466
VpaK11BI GGWCC 4 cut(s) 266, 566, 869, 932
XapI RAATTY 3 cut(s) 461, 794, 1059
XbaI TCTAGA 1 cut(s) 1024
XceI RCATGY 2 cut(s) 371, 1018
XspI CTAG 3 cut(s) 87, 710, 1025
ZrmI AGTACT 1 cut(s) 119
Zsp2I ATGCAT 2 cut(s) 415, 1020
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.