Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0002927.1

Orthogroup ID

OG0003661

Physical Location & Seq

Genomic Coordinates Reverse (-)

199485 .. 209447

Gene Structure 9963 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3915 bp

atggagcatcctcagactgtttcattaagtaaggtggttctgacaaatacttacttgaaagacgctcttggtaagaacaaacactcacagacaattctgcgttggaggcaactgcaagattgtataaatctaatgttcgatagcaaaactgttgcgggtcaaggtaaatcagatgcttctggaatatgtcagttgctggagaaaaaggaaggcatgttccgtcagaaaatgatgggaaagagggttaataatgcgtgccggtctgttatatccccagatccctatttagctgttaatgagattggaattcctccgatctttgcattgacattaacatatcctgagagagttactccgtggaatgttttcaagttgaggaatgctattgtcaatggttctgaaatccatcctggagctacgcactatattgataaaacatctattaagttgcctcaaaagcggagggagcgcttgttattgtcaagaaagttaccttcttcaaaaggggcagggacacaacatgggaagggatctgactatgaaaacaaaattgtactccgtcacttgcaagatggggacattgttcttgttaatcgccagcctacacttcacaaacccagtataatggctcatatagttcgtgtattgaagggggagaaaacattacgcatgcactatgcaaattgcagtacatacaatgctgattttgatggtgatgaaatgaatgttcatttcccacaagatgaaatttctcgcgctgaagcttacaatatggtgaacgcaaataatcaatacgtaaagcccactagtggtgatcccattcgagccttgattcaggaccatgttataagtgctgtcctcctcacaaaaaaggacacctttctccgctcagatgaatttaatcagcttctttatagttcttctgtttccgcatgtggttcagactctttctctggcaagccaggtcaaaaggttctaacgtctaactctgaagacgcaatgcagccccttcttcctgcaatttggaaacctgtgcgtctgtggactggaaagcaggtcatcactacgttgctaaaccatattacaagtggttctccaccacttagtgttgaaaaggaagcaaaactcccagctgggtttttcaactgtaagaatcttgaagaagaagagaaatctgataaaggtcgacgaaagaaagagaagatgccaagaaaagaagaggctggtaaagatgattcaaaggaaaaagaggatccagatgagagcaagctgttaatctatagaaacaacttggtccgtggcgtgattgacaagaaccagtttggtgattatggtttggtccataacgtgcaggaactctatggttcggaagctgcaggaaaattgctttctgtttttagtcgtttatatactgtttatttacagatgcatgggttcacttgtggagttgacgatcttttgctaatacaaagcaaagacaaaaaaatgaaggaacaacttgaaagttgtgaagagcttggtgaaaaagttttccgtgagtttattggggttggacaagatgaaaaaaaagatccggtgatactgcagtcggatattgaagaatttttacgcagtaatggagaatctgtgacggaaatgttggacaggaaaatgacaagtgaacttaataagaaaatctccaaatctgatgtcttcaaacaattgtttcagaaagggttatcaaagccatctgttaagaattatatgtatcttatgacaagctctggggctaaaggaggtgtggcaaatctccaacaaattgcttcatacttgggtcagcaagagctagaagggaaacgagtccctcaaatggtttctggaaagacattaccatgcttccctccttgggattggtcacctcgagctggaggttttattattgatcgctttctcaccggtcttcatcctcaggagtattattttcactgcatggctggccgtgaagggttggttgatacagctgtcaaaacatctcgtagtggatacttgcaaagatgcctaataaaaaatctcgaatgcctcaaggtttcttatgaccatactgtccgtgatgctgatggttcaattattcaattccgatatggagaggatggtattgatgtccatttgacaagctttattaaaaaatttgaaaaactggcaaataataaagaaatgtttgacaagaaatttggtcgtcaaattgacaaattcaatccctatattgatgagttgcctcccgctctcaaaagaaaagcagaaatgtcagttcctaatttgtctttgaaggagaaaaatgccaacttgaagttaatggagcacaaatatctcagcagtcttgctcaaccaggagaaccagttggtattcttgctgctcagtctgtaggagaaccatcaacacagatgactttgaataccttccataatgctgggcgaagtgaaatgaatgtgactcttggaattccacggctccaagagatcttgatgactgcttcaaatgatatcaagactccagttatgacatgtccattgataaaggagaaatcgggggatgctaaaagtctagctgataggttgaagaggatcactgtggcagacataatagagagcatgaaagtaactattacaccattcacagatcagtatcatcagacttgccgtacttatgaactggagatgacactttttataccagaatatctttcgaaattttcagaagattgggaggagctcctagaggttaagtttgttagggctttggaggatgccatacaaaatcatctggttttgctttctaaaattagtggtataaaagatgtcaaggctgattcactaccgaaggcatcaaatgagacagatgaagatgtttcttacaacatatcccagcatggtgtagaagatgatgacgatggcggtgcagatgacagtgaacatggtgaaggagctgaggattttgggttggatgcacaaaaacgaaagcggcagggcacagatgagatggaatatgaagatgactgtgaggatgaagcgaatgaaggtcatcagtccgatggatttgaaagcgaaattgataaggtggaaagtgatattgaaagtgataaggatggtgtgacacggatttcagatgctaatgatgaaatgcctgaaagtccttcagctggagacacatctaagccacggtcgaagggaaagaagaccaaatcaagggacaaaattaaaaagaagaccagagcaaagctagtaagaaaggagtatgacagggcaacttttgtgtcagtcaaggggttccactttgagatccattttaaatttaccaatgaacctcatattcttttggatcagattgctcggaagacggctcgaaatgtttatgtcaaaagctctggcaatattttggattgtaaagatattgatatttatggtaatgaagatcaagttttctgctcctggaatgatcccagaaataaattaccgggtgaaaagaatcccacggaagaagaaaagaagatgcgagccttgcagacagcagggttggattttgctacactatggaaactgcaagatgatcttgatgtcaactatatatattccaacaacattcatgcaatgctgaacacttatggagtagaagccgccagagaaactatcatcaaagaaataactgactacatgactcatagtggcggttatcgtccaatgaacagatttgggggtatagcagagtctgtatctccgatcaataaaatgtcatttgagacggcttccaagttcattcgtgaagcagcatatcatggggagacggatgacttggaaaccccttctgctaggatctgtcttgggctgccagtgaaagtgggtactggttgttttgatttgatgcaaaagatggagatttaa

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005736 GO:0005911 GO:0006139 GO:0006351 GO:0006360 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009987 GO:0010467 GO:0016070 GO:0018130 GO:0019438 GO:0030054 GO:0030880 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0055029 GO:0055044 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 map00230 map00240 map01100 map03020

Pfam Domains

Protein Families

RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5

Protein Analysis

1304

Amino Acids

147.01

Weight (kDa)

6.24

Isoelectric Point (pI)

44.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_2	PF00623	79 - 260	3.1e-58	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	264 - 486	1.8e-29	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	539 - 639	3.2e-20	RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5	PF04998	646 - 1234	3.5e-71	RNA polymerase Rpb1, domain 5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003661)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57660 AT3G57660
fragaria_vesca	FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g35030 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210
malus_domestica	MD12G1066600.v1.1 MD14G1066700.v1.1
prunus_persica	Prupe.7G067700_v2.0.a1 Prupe.7G067700_v2.0.a1 Prupe.7G067700_v2.0.a1
pyrus_communis	pycom12g05800 pycom14g05430
rosa_chinensis	RchiOBHm_Chr3g0481021
rosa_laevigata	RLG00000023431
rosa_multiflora	Rmu_sc0002927.1_g000058
rosa_roxburghii	Rroxscaffold_6G00400730
rosa_rugosa	Rorug03G0189100
rosa_samantha	Rh3AG240000 Rh3BG275100 Rh3CG270600 Rh3DG266500
rosa_wichuraiana	Rw0G008430 Rw3G021430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	848
AasI	GACNNNNNNGTC	1 cut(s)	2081
Acc36I	ACCTGC	1 cut(s)	1045
AccBSI	CCGCTC	2 cut(s)	888, 2261
AccI	GTMKAC	1 cut(s)	1186
AccII	CGCG	1 cut(s)	756
AciI	CCGC	9 cut(s)	155, 460, 886, 930, 2259, 2931, 2998, 3651, 3702
AcoI	YGGCCR	1 cut(s)	1975
AcuI	CTGAAG	3 cut(s)	780, 1011, 3156
AdeI	CACNNNGTG	1 cut(s)	1106
AfaI	GTAC	4 cut(s)	555, 691, 2680, 3877
AfeI	AGCGCT	1 cut(s)	470
AfiI	CCNNNNNNNGG	6 cut(s)	809, 1849, 1885, 1904, 3176, 3222
AflIII	ACRYGT	1 cut(s)	2540
AgeI	ACCGGT	1 cut(s)	1934
AhlI	ACTAGT	1 cut(s)	806
AjnI	CCWGG	4 cut(s)	409, 961, 2365, 3464
AloI	GAACNNNNNNTCC	2 cut(s)	200, 232
Alw21I	GWGCWC	2 cut(s)	2340, 2751
Alw26I	GTCTC	4 cut(s)	2864, 3174, 3767, 3809
AlwNI	CAGNNNCTG	2 cut(s)	196, 3743
Ama87I	CYCGRG	1 cut(s)	1899
Aor51HI	AGCGCT	1 cut(s)	470
AoxI	GGCC	1 cut(s)	1975
ApeKI	GCWGC	5 cut(s)	1003, 1373, 2390, 3800, 3859
ArsI	GACNNNNNNTTYG	4 cut(s)	1674, 1706, 2192, 2224
AsiGI	ACCGGT	1 cut(s)	1934
Asp700I	GAANNNNTTC	2 cut(s)	365, 2435
AspLEI	GCGC	2 cut(s)	471, 758
AspS9I	GGNCC	3 cut(s)	838, 1294, 1339
AsuC2I	CCSGG	1 cut(s)	3492
AsuII	TTCGAA	1 cut(s)	2723
AvaI	CYCGRG	1 cut(s)	1899
AvaII	GGWCC	3 cut(s)	838, 1294, 1339
AxyI	CCTNAGG	1 cut(s)	1947
BaeGI	GKGCMC	1 cut(s)	3008
BaeI	ACNNNNGTAYC	4 cut(s)	1571, 1604, 1986, 2019
BamHI	GGATCC	1 cut(s)	1252
BanII	GRGCYC	1 cut(s)	2751
BarI	GAAGNNNNNNTAC	2 cut(s)	1590, 1622
BbsI	GAAGAC	6 cut(s)	999, 1684, 1931, 3218, 3248, 3377
Bbv12I	GWGCWC	2 cut(s)	2340, 2751
BbvCI	CCTCAGC	1 cut(s)	2964
BbvI	GCAGC	5 cut(s)	1015, 1360, 2377, 3812, 3846
BceAI	ACGGC	5 cut(s)	1962, 2501, 2661, 3390, 3792
BcgI	CGANNNNNNTGC	2 cut(s)	2915, 2949
BciT130I	CCWGG	4 cut(s)	411, 963, 2367, 3466
BciVI	GTATCC	1 cut(s)	2015
BcnI	CCSGG	1 cut(s)	3492
BcoDI	GTCTC	4 cut(s)	2864, 3174, 3767, 3809
BcuI	ACTAGT	1 cut(s)	806
BfaI	CTAG	6 cut(s)	807, 1826, 2582, 2753, 3257, 3843
BfmI	CTRYAG	4 cut(s)	1279, 1374, 1583, 2400
BfoI	RGCGCY	1 cut(s)	472
BfuAI	ACCTGC	1 cut(s)	1045
BfuI	GTATCC	1 cut(s)	2015
BglII	AGATCT	1 cut(s)	2496
BisI	GCNGC	7 cut(s)	1004, 1374, 2391, 2999, 3651, 3801, 3860
BlsI	GCNGC	7 cut(s)	1005, 1375, 2392, 3000, 3652, 3802, 3861
Bme1390I	CCNGG	5 cut(s)	411, 963, 2367, 3466, 3492
Bme18I	GGWCC	3 cut(s)	838, 1294, 1339
BmeT110I	CYCGRG	1 cut(s)	1899
BmgT120I	GGNCC	3 cut(s)	838, 1294, 1339
BmiI	GGNNCC	3 cut(s)	1254, 2489, 3305
BmrFI	CCNGG	5 cut(s)	411, 963, 2367, 3466, 3492
BmrI	ACTGGG	1 cut(s)	612
BmuI	ACTGGG	1 cut(s)	612
BpiI	GAAGAC	6 cut(s)	999, 1684, 1931, 3218, 3248, 3377
BplI	GAGNNNNNCTC	2 cut(s)	337, 369
BpmI	CTGGAG	6 cut(s)	218, 432, 1926, 2514, 2711, 3198
Bpu10I	CCTNAGC	1 cut(s)	2964
Bpu14I	TTCGAA	1 cut(s)	2723
BpuEI	CTTGAG	1 cut(s)	2045
BpuMI	CCSGG	1 cut(s)	3492
BsaAI	YACGTR	1 cut(s)	796
BsaBI	GATNNNNATC	1 cut(s)	2661
BsaJI	CCNNGG	6 cut(s)	356, 1297, 1883, 2483, 3194, 3507
BsaWI	WCCGGW	2 cut(s)	1573, 1934
BsaXI	ACNNNNNCTCC	6 cut(s)	458, 488, 1611, 1641, 2361, 2391
Bsc4I	CCNNNNNNNGG	6 cut(s)	809, 1849, 1885, 1904, 3176, 3222
Bse118I	RCCGGY	2 cut(s)	258, 1934
Bse1I	ACTGG	9 cut(s)	618, 1051, 1318, 2181, 2375, 2531, 2694, 3863, 3883
Bse21I	CCTNAGG	1 cut(s)	1947
Bse3DI	GCAATG	2 cut(s)	1005, 3630
Bse8I	GATNNNNATC	1 cut(s)	2661
BseBI	CCWGG	4 cut(s)	411, 963, 2367, 3466
BseDI	CCNNGG	6 cut(s)	356, 1297, 1883, 2483, 3194, 3507
BseJI	GATNNNNATC	1 cut(s)	2661
BseLI	CCNNNNNNNGG	6 cut(s)	809, 1849, 1885, 1904, 3176, 3222
BseMI	GCAATG	2 cut(s)	1005, 3630
BseMII	CTCAG	7 cut(s)	26, 333, 903, 1961, 2362, 2408, 2955
BseNI	ACTGG	9 cut(s)	618, 1051, 1318, 2181, 2375, 2531, 2694, 3863, 3883
BseRI	GAGGAG	2 cut(s)	851, 2759
BseSI	GKGCMC	1 cut(s)	3008
BseXI	GCAGC	5 cut(s)	1015, 1360, 2377, 3812, 3846
BseYI	CCCAGC	4 cut(s)	1129, 1133, 2447, 2901
BsgI	GTGCAG	2 cut(s)	1370, 2955
Bsh1236I	CGCG	1 cut(s)	756
Bsh1285I	CGRYCG	1 cut(s)	3200
BshFI	GGCC	1 cut(s)	1977
BshTI	ACCGGT	1 cut(s)	1934
BsiEI	CGRYCG	1 cut(s)	3200
BsiHKAI	GWGCWC	2 cut(s)	2340, 2751
BsiHKCI	CYCGRG	1 cut(s)	1899
BsiSI	CCGG	4 cut(s)	259, 1574, 1935, 3491
BslFI	GGGAC	4 cut(s)	526, 590, 1826, 3239
BslI	CCNNNNNNNGG	6 cut(s)	809, 1849, 1885, 1904, 3176, 3222
BsmAI	GTCTC	4 cut(s)	2864, 3174, 3767, 3809
BsmBI	CGTCTC	2 cut(s)	3767, 3809
BsmFI	GGGAC	4 cut(s)	526, 590, 1826, 3239
BsmI	GAATGC	2 cut(s)	385, 2060
BsnI	GGCC	1 cut(s)	1977
BsoBI	CYCGRG	1 cut(s)	1899
Bsp119I	TTCGAA	1 cut(s)	2723
Bsp1286I	GDGCHC	3 cut(s)	2340, 2751, 3008
BspACI	CCGC	9 cut(s)	155, 460, 886, 930, 2259, 2931, 2998, 3651, 3702
BspANI	GGCC	1 cut(s)	1977
BspCNI	CTCAG	7 cut(s)	25, 334, 902, 1960, 2361, 2407, 2956
BspFNI	CGCG	1 cut(s)	756
BspLI	GGNNCC	3 cut(s)	1254, 2489, 3305
BspMAI	CTGCAG	2 cut(s)	1378, 1587
BspMI	ACCTGC	1 cut(s)	1045
BspQI	GCTCTTC	1 cut(s)	1506
BspT104I	TTCGAA	1 cut(s)	2723
BsrBI	CCGCTC	2 cut(s)	888, 2261
BsrDI	GCAATG	2 cut(s)	1005, 3630
BsrFI	RCCGGY	2 cut(s)	258, 1934
BsrI	ACTGG	9 cut(s)	618, 1051, 1318, 2181, 2375, 2531, 2694, 3863, 3883
BssAI	RCCGGY	2 cut(s)	258, 1934
BssECI	CCNNGG	6 cut(s)	356, 1297, 1883, 2483, 3194, 3507
BssT1I	CCWWGG	1 cut(s)	1883
Bst2UI	CCWGG	4 cut(s)	411, 963, 2367, 3466
Bst4CI	ACNGT	9 cut(s)	19, 151, 1148, 1414, 2083, 2608, 2945, 3035, 3198
Bst6I	CTCTTC	4 cut(s)	1161, 1212, 1506, 2591
BstBAI	YACGTR	1 cut(s)	796
BstBI	TTCGAA	1 cut(s)	2723
BstC8I	GCNNGC	7 cut(s)	256, 599, 671, 959, 1268, 1975, 3531
BstDEI	CTNAG	9 cut(s)	12, 342, 889, 1103, 1947, 2348, 2394, 2964, 3189
BstDSI	CCRYGG	5 cut(s)	356, 1297, 2483, 3194, 3507
BstEII	GGTNACC	1 cut(s)	1893
BstFNI	CGCG	1 cut(s)	756
BstH2I	RGCGCY	1 cut(s)	472
BstHHI	GCGC	2 cut(s)	471, 758
BstMAI	GTCTC	4 cut(s)	2864, 3174, 3767, 3809
BstMCI	CGRYCG	1 cut(s)	3200
BstMWI	GCNNNNNNNGC	5 cut(s)	106, 457, 466, 1974, 3535
BstNI	CCWGG	4 cut(s)	411, 963, 2367, 3466
BstNSI	RCATGY	4 cut(s)	217, 673, 936, 2544
BstPI	GGTNACC	1 cut(s)	1893
BstSCI	CCNGG	5 cut(s)	409, 961, 2365, 3464, 3490
BstSFI	CTRYAG	4 cut(s)	1279, 1374, 1583, 2400
BstSLI	GKGCMC	1 cut(s)	3008
BstSNI	TACGTA	1 cut(s)	796
BstUI	CGCG	1 cut(s)	756
BstV1I	GCAGC	5 cut(s)	1015, 1360, 2377, 3812, 3846
BstV2I	GAAGAC	6 cut(s)	999, 1684, 1931, 3218, 3248, 3377
BstX2I	RGATCY	7 cut(s)	277, 530, 1252, 1570, 2496, 3315, 3846
BstXI	CCANNNNNNTGG	2 cut(s)	625, 2447
BstYI	RGATCY	7 cut(s)	277, 530, 1252, 1570, 2496, 3315, 3846
Bsu36I	CCTNAGG	1 cut(s)	1947
BsuI	GTATCC	1 cut(s)	2015
BsuRI	GGCC	1 cut(s)	1977
BtgI	CCRYGG	5 cut(s)	356, 1297, 2483, 3194, 3507
BtsI	GCAGTG	1 cut(s)	1963
BtsIMutI	CAGTG	4 cut(s)	1963, 2604, 2950, 3870
BveI	ACCTGC	1 cut(s)	1045
Cac8I	GCNNGC	7 cut(s)	256, 599, 671, 959, 1268, 1975, 3531
CaiI	CAGNNNCTG	2 cut(s)	196, 3743
CfoI	GCGC	2 cut(s)	471, 758
Cfr10I	RCCGGY	2 cut(s)	258, 1934
Cfr13I	GGNCC	3 cut(s)	838, 1294, 1339
CseI	GACGC	3 cut(s)	71, 1004, 1025
Csp6I	GTAC	4 cut(s)	554, 690, 2679, 3876
CspAI	ACCGGT	1 cut(s)	1934
CviQI	GTAC	4 cut(s)	554, 690, 2679, 3876
DdeI	CTNAG	9 cut(s)	12, 342, 889, 1103, 1947, 2348, 2394, 2964, 3189
DraI	TTTAAA	1 cut(s)	3325
DraIII	CACNNNGTG	1 cut(s)	1106
DrdI	GACNNNNNNGTC	1 cut(s)	2081
DseDI	GACNNNNNNGTC	1 cut(s)	2081
EaeI	YGGCCR	1 cut(s)	1975
Eam1104I	CTCTTC	4 cut(s)	1161, 1212, 1506, 2591
EarI	CTCTTC	4 cut(s)	1161, 1212, 1506, 2591
Ecl136II	GAGCTC	1 cut(s)	2749
Eco105I	TACGTA	1 cut(s)	796
Eco130I	CCWWGG	1 cut(s)	1883
Eco24I	GRGCYC	1 cut(s)	2751
Eco32I	GATATC	1 cut(s)	2521
Eco47I	GGWCC	3 cut(s)	838, 1294, 1339
Eco47III	AGCGCT	1 cut(s)	470
Eco53kI	GAGCTC	1 cut(s)	2749
Eco57I	CTGAAG	3 cut(s)	780, 1011, 3156
Eco81I	CCTNAGG	1 cut(s)	1947
Eco88I	CYCGRG	1 cut(s)	1899
Eco91I	GGTNACC	1 cut(s)	1893
EcoICRI	GAGCTC	1 cut(s)	2749
EcoO65I	GGTNACC	1 cut(s)	1893
EcoRI	GAATTC	2 cut(s)	306, 2478
EcoRII	CCWGG	4 cut(s)	409, 961, 2365, 3464
EcoRV	GATATC	1 cut(s)	2521
EcoT14I	CCWWGG	1 cut(s)	1883
EcoT22I	ATGCAT	1 cut(s)	1431
EcoT38I	GRGCYC	1 cut(s)	2751
ErhI	CCWWGG	1 cut(s)	1883
Esp3I	CGTCTC	2 cut(s)	3767, 3809
FalI	AAGNNNNNCTT	6 cut(s)	51, 83, 863, 895, 3570, 3602
FaqI	GGGAC	4 cut(s)	526, 590, 1826, 3239
FauI	CCCGC	2 cut(s)	148, 2266
FblI	GTMKAC	1 cut(s)	1186
Fnu4HI	GCNGC	7 cut(s)	1004, 1374, 2391, 2999, 3651, 3801, 3860
FokI	GGATG	9 cut(s)	393, 1929, 2141, 2582, 2795, 2993, 3053, 3134, 3833
FriOI	GRGCYC	1 cut(s)	2751
Fsp4HI	GCNGC	7 cut(s)	1004, 1374, 2391, 2999, 3651, 3801, 3860
FspBI	CTAG	6 cut(s)	807, 1826, 2582, 2753, 3257, 3843
GlaI	GCGC	2 cut(s)	470, 757
GluI	GCNGC	7 cut(s)	1004, 1374, 2391, 2999, 3651, 3801, 3860
GsaI	CCCAGC	4 cut(s)	1133, 1137, 2451, 2905
GsuI	CTGGAG	6 cut(s)	218, 432, 1926, 2514, 2711, 3198
HaeII	RGCGCY	1 cut(s)	472
HaeIII	GGCC	1 cut(s)	1977
HapII	CCGG	4 cut(s)	259, 1574, 1935, 3491
HgaI	GACGC	3 cut(s)	71, 1004, 1025
HhaI	GCGC	2 cut(s)	471, 758
Hin6I	GCGC	2 cut(s)	469, 756
HinP1I	GCGC	2 cut(s)	469, 756
HincII	GTYRAC	3 cut(s)	1187, 1450, 3595
HindII	GTYRAC	3 cut(s)	1187, 1450, 3595
HindIII	AAGCTT	2 cut(s)	762, 2152
HpaII	CCGG	4 cut(s)	259, 1574, 1935, 3491
Hpy166II	GTNNAC	8 cut(s)	778, 1044, 1187, 1437, 1450, 1661, 2948, 3595
Hpy8I	GTNNAC	8 cut(s)	778, 1044, 1187, 1437, 1450, 1661, 2948, 3595
Hpy99I	CGWCG	1 cut(s)	1191
HpyCH4III	ACNGT	9 cut(s)	19, 151, 1148, 1414, 2083, 2608, 2945, 3035, 3198
HpyCH4IV	ACGT	4 cut(s)	795, 980, 1067, 1347
HpyF10VI	GCNNNNNNNGC	5 cut(s)	106, 457, 466, 1974, 3535
HpyF3I	CTNAG	9 cut(s)	12, 342, 889, 1103, 1947, 2348, 2394, 2964, 3189
HpySE526I	ACGT	4 cut(s)	795, 980, 1067, 1347
HspAI	GCGC	2 cut(s)	469, 756
LguI	GCTCTTC	1 cut(s)	1506
LmnI	GCTCC	9 cut(s)	4, 413, 466, 2335, 2493, 2746, 2754, 2960, 3467
Lsp1109I	GCAGC	5 cut(s)	1015, 1360, 2377, 3812, 3846
MaeI	CTAG	6 cut(s)	807, 1826, 2582, 2753, 3257, 3843
MaeII	ACGT	4 cut(s)	795, 980, 1067, 1347
MaeIII	GTNAC	8 cut(s)	349, 489, 560, 1627, 1893, 2467, 2635, 3127
MbiI	CCGCTC	2 cut(s)	888, 2261
MfeI	CAATTG	1 cut(s)	1700
MflI	RGATCY	7 cut(s)	277, 530, 1252, 1570, 2496, 3315, 3846
MhlI	GDGCHC	3 cut(s)	2340, 2751, 3008
MlyI	GAGTC	6 cut(s)	938, 1848, 2464, 2521, 3685, 3749
MmeI	TCCRAC	8 cut(s)	83, 1531, 1569, 1620, 1816, 2958, 3531, 3633
Mph1103I	ATGCAT	1 cut(s)	1431
MroXI	GAANNNNTTC	2 cut(s)	365, 2435
MslI	CAYNNNNRTG	1 cut(s)	1870
MspA1I	CMGCKG	3 cut(s)	1133, 2000, 3176
MspI	CCGG	4 cut(s)	259, 1574, 1935, 3491
MspR9I	CCNGG	5 cut(s)	411, 963, 2367, 3466, 3492
MunI	CAATTG	1 cut(s)	1700
Mva1269I	GAATGC	2 cut(s)	385, 2060
MvaI	CCWGG	4 cut(s)	411, 963, 2367, 3466
MvnI	CGCG	1 cut(s)	756
MwoI	GCNNNNNNNGC	5 cut(s)	106, 457, 466, 1974, 3535
NciI	CCSGG	1 cut(s)	3492
NlaIV	GGNNCC	3 cut(s)	1254, 2489, 3305
NmuCI	GTSAC	5 cut(s)	560, 1627, 1893, 2467, 3127
NsiI	ATGCAT	1 cut(s)	1431
NspI	RCATGY	4 cut(s)	217, 673, 936, 2544
NspV	TTCGAA	1 cut(s)	2723
PaeI	GCATGC	1 cut(s)	673
PaeR7I	CTCGAG	1 cut(s)	1899
PciI	ACATGT	1 cut(s)	2540
PciSI	GCTCTTC	1 cut(s)	1506
PctI	GAATGC	2 cut(s)	385, 2060
PdmI	GAANNNNTTC	2 cut(s)	365, 2435
PfeI	GAWTC	6 cut(s)	832, 1153, 1235, 1622, 2846, 3502
PfoI	TCCNGGA	2 cut(s)	409, 3464
PinAI	ACCGGT	1 cut(s)	1934
PkrI	GCNGC	7 cut(s)	1005, 1375, 2392, 3000, 3652, 3802, 3861
PleI	GAGTC	6 cut(s)	938, 1847, 2464, 2521, 3685, 3748
PpsI	GAGTC	6 cut(s)	938, 1847, 2464, 2521, 3685, 3748
Ppu21I	YACGTR	1 cut(s)	796
PscI	ACATGT	1 cut(s)	2540
PsiI	TTATAA	1 cut(s)	848
Psp124BI	GAGCTC	1 cut(s)	2751
Psp6I	CCWGG	4 cut(s)	409, 961, 2365, 3464
PspEI	GGTNACC	1 cut(s)	1893
PspFI	CCCAGC	4 cut(s)	1129, 1133, 2447, 2901
PspGI	CCWGG	4 cut(s)	409, 961, 2365, 3464
PspN4I	GGNNCC	3 cut(s)	1254, 2489, 3305
PspPI	GGNCC	3 cut(s)	838, 1294, 1339
PspXI	VCTCGAGB	1 cut(s)	1899
PstI	CTGCAG	2 cut(s)	1378, 1587
PstNI	CAGNNNCTG	2 cut(s)	196, 3743
PsuI	RGATCY	7 cut(s)	277, 530, 1252, 1570, 2496, 3315, 3846
PvuII	CAGCTG	3 cut(s)	1133, 2000, 3176
RsaI	GTAC	4 cut(s)	555, 691, 2680, 3877
RsaNI	GTAC	4 cut(s)	554, 690, 2679, 3876
RseI	CAYNNNNRTG	1 cut(s)	1870
SacI	GAGCTC	1 cut(s)	2751
SalI	GTCGAC	1 cut(s)	1185
SapI	GCTCTTC	1 cut(s)	1506
SatI	GCNGC	7 cut(s)	1004, 1374, 2391, 2999, 3651, 3801, 3860
Sau96I	GGNCC	3 cut(s)	838, 1294, 1339
SchI	GAGTC	6 cut(s)	938, 1848, 2464, 2521, 3685, 3749
ScrFI	CCNGG	5 cut(s)	411, 963, 2367, 3466, 3492
SduI	GDGCHC	3 cut(s)	2340, 2751, 3008
SfcI	CTRYAG	4 cut(s)	1279, 1374, 1583, 2400
Sfr274I	CTCGAG	1 cut(s)	1899
SfuI	TTCGAA	1 cut(s)	2723
SinI	GGWCC	3 cut(s)	838, 1294, 1339
SlaI	CTCGAG	1 cut(s)	1899
SmiMI	CAYNNNNRTG	1 cut(s)	1870
SmlI	CTYRAG	2 cut(s)	1899, 2060
SmoI	CTYRAG	2 cut(s)	1899, 2060
SnaBI	TACGTA	1 cut(s)	796
SpeI	ACTAGT	1 cut(s)	806
SphI	GCATGC	1 cut(s)	673
SsiI	CCGC	9 cut(s)	155, 460, 886, 930, 2259, 2931, 2998, 3651, 3702
SspI	AATATT	1 cut(s)	3409
SspMI	CTAG	6 cut(s)	807, 1826, 2582, 2753, 3257, 3843
SstI	GAGCTC	1 cut(s)	2751
StyD4I	CCNGG	5 cut(s)	409, 961, 2365, 3464, 3490
StyI	CCWWGG	1 cut(s)	1883
TaaI	ACNGT	9 cut(s)	19, 151, 1148, 1414, 2083, 2608, 2945, 3035, 3198
TaiI	ACGT	4 cut(s)	798, 983, 1070, 1350
TaqI	TCGA	8 cut(s)	138, 823, 1186, 1900, 2052, 2723, 3200, 3379
TatI	WGTACW	2 cut(s)	553, 689
TauI	GCSGC	2 cut(s)	3001, 3653
TfiI	GAWTC	6 cut(s)	832, 1153, 1235, 1622, 2846, 3502
TscAI	CASTG	4 cut(s)	1970, 2611, 2950, 3870
TseFI	GTSAC	5 cut(s)	560, 1627, 1893, 2467, 3127
TseI	GCWGC	5 cut(s)	1003, 1373, 2390, 3800, 3859
Tsp45I	GTSAC	5 cut(s)	560, 1627, 1893, 2467, 3127
TspRI	CASTG	4 cut(s)	1970, 2611, 2950, 3870
VpaK11BI	GGWCC	3 cut(s)	838, 1294, 1339
XceI	RCATGY	4 cut(s)	217, 673, 936, 2544
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1085
XhoI	CTCGAG	1 cut(s)	1899
XmiI	GTMKAC	1 cut(s)	1186
XmnI	GAANNNNTTC	2 cut(s)	365, 2435
XspI	CTAG	6 cut(s)	807, 1826, 2582, 2753, 3257, 3843
Zsp2I	ATGCAT	1 cut(s)	1431

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.