Rmu_sc0003127.1_g000018

Belongs to the phosphoglycerate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003127.1
OG0004015
Physical Location & Seq
Reverse (-)
86478 .. 90665
4188 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1167 bp
atggttctttcctctcaagccccacctaaggcgggggttgatctcaatgttcctttggatgacgactgtaagatcaccgatgacactagagtccgagcagctgtccccaccatcaagtacttgctcggaaatggatgtcccaagggtgtcactcccaagtacagtttgaagcctcttgtgccaagactgtctgaacttcttggcattcaggtcgtgaaggctgaagactttattggtccagaagtagaaaagctggcggcttcacttcctgatggtgctgtccttcttcttgaaaatgtgaggttttacaaagaggaggagaagaacgatcctgagcatgcaaagaagctcgcctctgtagctgatctttttgtcaatgatgcattcggaactgcacatagaacccatgcttcaactgagggtgtaacaaaattcttaaggccatctgtagctggttttcttttgcagaaggaactcgactatgttattggggtagtatcaaacccaaaaaagccatttgcagccgttgttggtggtccgaaggtctcatccaagattggagtgatcgagtcactgttagagatggttcattacttaattcttggtggaggaatgatcttcacattttacaaggcacagggtatctcagtgggttcatctctgctggaaaaggataagctagaactcgctacatcactcattgcaaaggccaaggaaaagggagtgtctcttttgctatccactgatattattccagcttcaagcatccctgatgggtggatgggattggatattggaccacactctattaagacattcaatgatgcacttgataccactcaaaccatcatttggaacggaccaatgggagtgtctgagtttgacaagtttgcagtaggaacagagacatggttgttgtgcctcttatgtatgctagccattgcacgagatggagctagtggtggtgttgtccgcacggttgtgtattacagcggtgggcacttgagtggctcaactaacagtggcactaatgacgcaaagaaaattggtgctgaagaagattttgagaattttcttggttgcttcaaagatacgaatatgcaagcccaaggtgtggatcccaagaggggttggggatttcgtggtgtgcacaaggtattgttgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002682 GO:0002697 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006464 GO:0006468 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009941 GO:0009987 GO:0010035 GO:0010038 GO:0010319 GO:0015977 GO:0015979 GO:0016020 GO:0016051 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019253 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019538 GO:0019637 GO:0019685 GO:0019693 GO:0019752 GO:0030312 GO:0031347 GO:0031967 GO:0031975 GO:0032101 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0036211 GO:0042221 GO:0042866 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043900 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044267 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046939 GO:0048046 GO:0048583 GO:0050688 GO:0050691 GO:0050789 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0065007 GO:0071704 GO:0071944 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0080134 GO:0090407 GO:0140096 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PGK

Protein Analysis

388

Amino Acids

41.58

Weight (kDa)

6.03

Isoelectric Point (pI)

22.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGK PF00162 13 - 304 7.7e-106 Phosphoglycerate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 89
AccB7I CCANNNNNTGG 2 cut(s) 852, 1114
AciI CCGC 4 cut(s) 32, 257, 973, 993
AclWI GGATC 3 cut(s) 323, 1112, 1125
AcsI RAATTY 2 cut(s) 431, 1069
AcuI CTGAAG 2 cut(s) 243, 1074
AfaI GTAC 2 cut(s) 119, 161
AfiI CCNNNNNNNGG 5 cut(s) 28, 32, 852, 1114, 1127
AflII CTTAAG 1 cut(s) 436
AgsI TTSAA 6 cut(s) 169, 293, 414, 762, 820, 1087
AjuI GAANNNNNNNTTGG 2 cut(s) 216, 248
AleI CACNNNNGTG 2 cut(s) 980, 1005
AluBI AGCT 8 cut(s) 101, 253, 349, 362, 452, 679, 758, 956
AluI AGCT 8 cut(s) 101, 253, 349, 362, 452, 679, 758, 956
Alw21I GWGCWC 1 cut(s) 1152
Alw26I GTCTC 3 cut(s) 550, 732, 899
Alw44I GTGCAC 1 cut(s) 1148
AlwI GGATC 3 cut(s) 323, 1112, 1125
AoxI GGCC 2 cut(s) 440, 708
ApaLI GTGCAC 1 cut(s) 1148
ApeKI GCWGC 2 cut(s) 98, 521
ApoI RAATTY 2 cut(s) 431, 1069
AspS9I GGNCC 4 cut(s) 236, 536, 797, 860
AsuHPI GGTGA 1 cut(s) 67
AsuNHI GCTAGC 1 cut(s) 934
AvaII GGWCC 4 cut(s) 236, 536, 797, 860
AxyI CCTNAGG 1 cut(s) 27
BaeGI GKGCMC 2 cut(s) 1002, 1152
BamHI GGATCC 1 cut(s) 1117
BauI CACGAG 1 cut(s) 945
BbsI GAAGAC 1 cut(s) 231
Bbv12I GWGCWC 1 cut(s) 1152
BbvI GCAGC 2 cut(s) 110, 533
BccI CCATC 8 cut(s) 119, 266, 451, 577, 767, 775, 854, 944
BceAI ACGGC 1 cut(s) 509
BcgI CGANNNNNNTGC 2 cut(s) 193, 227
BcoDI GTCTC 3 cut(s) 550, 732, 899
BfaI CTAG 4 cut(s) 87, 680, 935, 957
BfmI CTRYAG 2 cut(s) 357, 447
BfrI CTTAAG 1 cut(s) 436
BisI GCNGC 3 cut(s) 99, 258, 522
BlsI GCNGC 3 cut(s) 100, 259, 523
BmcAI AGTACT 1 cut(s) 119
Bme18I GGWCC 4 cut(s) 236, 536, 797, 860
BmgT120I GGNCC 4 cut(s) 236, 536, 797, 860
BmiI GGNNCC 1 cut(s) 1119
BmsI GCATC 3 cut(s) 370, 774, 814
BmtI GCTAGC 1 cut(s) 938
BpiI GAAGAC 1 cut(s) 231
Bpu10I CCTNAGC 1 cut(s) 333
BpuEI CTTGAG 1 cut(s) 1024
BsaI GGTCTC 1 cut(s) 550
BsaJI CCNNGG 3 cut(s) 141, 711, 1108
Bsc4I CCNNNNNNNGG 5 cut(s) 28, 32, 852, 1114, 1127
Bse21I CCTNAGG 1 cut(s) 27
Bse3DI GCAATG 2 cut(s) 699, 939
BseDI CCNNGG 3 cut(s) 141, 711, 1108
BseGI GGATG 5 cut(s) 64, 140, 548, 765, 786
BseLI CCNNNNNNNGG 5 cut(s) 28, 32, 852, 1114, 1127
BseMI GCAATG 2 cut(s) 699, 939
BseMII CTCAG 4 cut(s) 324, 408, 660, 867
BseRI GAGGAG 2 cut(s) 329, 332
BseSI GKGCMC 2 cut(s) 1002, 1152
BseXI GCAGC 2 cut(s) 110, 533
BsgI GTGCAG 1 cut(s) 378
BshFI GGCC 2 cut(s) 442, 710
BsiHKAI GWGCWC 1 cut(s) 1152
BslFI GGGAC 2 cut(s) 89, 123
BslI CCNNNNNNNGG 5 cut(s) 28, 32, 852, 1114, 1127
BsmAI GTCTC 3 cut(s) 550, 732, 899
BsmFI GGGAC 2 cut(s) 89, 123
BsmI GAATGC 2 cut(s) 204, 383
BsnI GGCC 2 cut(s) 442, 710
Bso31I GGTCTC 1 cut(s) 550
Bsp1286I GDGCHC 2 cut(s) 1002, 1152
Bsp143I GATC 7 cut(s) 40, 72, 328, 364, 564, 615, 1117
BspACI CCGC 4 cut(s) 32, 257, 973, 993
BspANI GGCC 2 cut(s) 442, 710
BspCNI CTCAG 4 cut(s) 325, 409, 659, 868
BspLI GGNNCC 1 cut(s) 1119
BspOI GCTAGC 1 cut(s) 938
BspPI GGATC 3 cut(s) 323, 1112, 1125
BspTI CTTAAG 1 cut(s) 436
BspTNI GGTCTC 1 cut(s) 550
BsrDI GCAATG 2 cut(s) 699, 939
BssECI CCNNGG 3 cut(s) 141, 711, 1108
BssMI GATC 7 cut(s) 40, 72, 328, 364, 564, 615, 1117
BssSI CACGAG 1 cut(s) 945
BssT1I CCWWGG 3 cut(s) 141, 711, 1108
Bst2BI CACGAG 1 cut(s) 945
Bst4CI ACNGT 6 cut(s) 68, 164, 189, 576, 979, 1022
BstAFI CTTAAG 1 cut(s) 436
BstC8I GCNNGC 5 cut(s) 255, 339, 351, 936, 1104
BstDEI CTNAG 5 cut(s) 27, 333, 417, 646, 876
BstF5I GGATG 5 cut(s) 64, 140, 548, 765, 786
BstKTI GATC 7 cut(s) 43, 75, 331, 367, 567, 618, 1120
BstMAI GTCTC 3 cut(s) 550, 732, 899
BstMBI GATC 7 cut(s) 40, 72, 328, 364, 564, 615, 1117
BstMWI GCNNNNNNNGC 2 cut(s) 178, 359
BstNSI RCATGY 1 cut(s) 341
BstSFI CTRYAG 2 cut(s) 357, 447
BstSLI GKGCMC 2 cut(s) 1002, 1152
BstV1I GCAGC 2 cut(s) 110, 533
BstV2I GAAGAC 1 cut(s) 231
BstX2I RGATCY 1 cut(s) 1117
BstYI RGATCY 1 cut(s) 1117
Bsu36I CCTNAGG 1 cut(s) 27
BsuRI GGCC 2 cut(s) 442, 710
BtsCI GGATG 5 cut(s) 64, 140, 548, 765, 786
BtsIMutI CAGTG 4 cut(s) 572, 654, 741, 1027
Cac8I GCNNGC 5 cut(s) 255, 339, 351, 936, 1104
Cfr13I GGNCC 4 cut(s) 236, 536, 797, 860
CseI GACGC 1 cut(s) 1043
Csp6I GTAC 2 cut(s) 118, 160
CviAII CATG 3 cut(s) 338, 407, 909
CviQI GTAC 2 cut(s) 118, 160
DdeI CTNAG 5 cut(s) 27, 333, 417, 646, 876
DpnI GATC 7 cut(s) 42, 74, 330, 366, 566, 617, 1119
DpnII GATC 7 cut(s) 40, 72, 328, 364, 564, 615, 1117
DrdI GACNNNNNNGTC 1 cut(s) 89
DseDI GACNNNNNNGTC 1 cut(s) 89
Eco130I CCWWGG 3 cut(s) 141, 711, 1108
Eco31I GGTCTC 1 cut(s) 550
Eco47I GGWCC 4 cut(s) 236, 536, 797, 860
Eco57I CTGAAG 2 cut(s) 243, 1074
Eco81I CCTNAGG 1 cut(s) 27
EcoT14I CCWWGG 3 cut(s) 141, 711, 1108
EcoT22I ATGCAT 1 cut(s) 385
ErhI CCWWGG 3 cut(s) 141, 711, 1108
FaeI CATG 3 cut(s) 341, 410, 912
FaiI YATR 8 cut(s) 339, 399, 408, 483, 910, 928, 932, 1100
FaqI GGGAC 2 cut(s) 89, 123
FatI CATG 3 cut(s) 337, 406, 908
FauI CCCGC 1 cut(s) 25
Fnu4HI GCNGC 3 cut(s) 99, 258, 522
FokI GGATG 5 cut(s) 71, 147, 535, 752, 793
Fsp4HI GCNGC 3 cut(s) 99, 258, 522
FspBI CTAG 4 cut(s) 87, 680, 935, 957
GluI GCNGC 3 cut(s) 99, 258, 522
HaeIII GGCC 2 cut(s) 442, 710
HgaI GACGC 1 cut(s) 1043
Hin1II CATG 3 cut(s) 341, 410, 912
HinfI GANTC 2 cut(s) 90, 569
HphI GGTGA 1 cut(s) 67
Hpy166II GTNNAC 1 cut(s) 1150
Hpy188I TCNGA 6 cut(s) 95, 128, 193, 389, 540, 877
Hpy188III TCNNGA 5 cut(s) 214, 239, 269, 290, 332
Hpy8I GTNNAC 1 cut(s) 1150
HpyAV CCTTC 4 cut(s) 211, 293, 463, 535
HpyCH4III ACNGT 6 cut(s) 68, 164, 189, 576, 979, 1022
HpyF10VI GCNNNNNNNGC 2 cut(s) 178, 359
HpyF3I CTNAG 5 cut(s) 27, 333, 417, 646, 876
Hsp92II CATG 3 cut(s) 341, 410, 912
Kzo9I GATC 7 cut(s) 40, 72, 328, 364, 564, 615, 1117
LmnI GCTCC 1 cut(s) 953
Lsp1109I GCAGC 2 cut(s) 110, 533
LweI GCATC 3 cut(s) 370, 774, 814
MaeI CTAG 4 cut(s) 87, 680, 935, 957
MaeIII GTNAC 3 cut(s) 148, 424, 570
MalI GATC 7 cut(s) 42, 74, 330, 366, 566, 617, 1119
MboI GATC 7 cut(s) 40, 72, 328, 364, 564, 615, 1117
MboII GAAGA 6 cut(s) 236, 278, 334, 610, 1067, 1070
MflI RGATCY 1 cut(s) 1117
MhlI GDGCHC 2 cut(s) 1002, 1152
MluCI AATT 4 cut(s) 431, 597, 1044, 1069
MlyI GAGTC 2 cut(s) 99, 578
Mph1103I ATGCAT 1 cut(s) 385
MseI TTAA 3 cut(s) 437, 596, 810
MslI CAYNNNNRTG 2 cut(s) 980, 1005
MspA1I CMGCKG 2 cut(s) 101, 993
MspCI CTTAAG 1 cut(s) 436
Mva1269I GAATGC 2 cut(s) 204, 383
MwoI GCNNNNNNNGC 2 cut(s) 178, 359
NdeII GATC 7 cut(s) 40, 72, 328, 364, 564, 615, 1117
NheI GCTAGC 1 cut(s) 934
NlaIII CATG 3 cut(s) 341, 410, 912
NlaIV GGNNCC 1 cut(s) 1119
NmuCI GTSAC 2 cut(s) 148, 570
NsiI ATGCAT 1 cut(s) 385
NspI RCATGY 1 cut(s) 341
OliI CACNNNNGTG 2 cut(s) 980, 1005
PaeI GCATGC 1 cut(s) 341
PctI GAATGC 2 cut(s) 204, 383
PflMI CCANNNNNTGG 2 cut(s) 852, 1114
PkrI GCNGC 3 cut(s) 100, 259, 523
PleI GAGTC 2 cut(s) 98, 577
PpsI GAGTC 2 cut(s) 98, 577
PspN4I GGNNCC 1 cut(s) 1119
PspPI GGNCC 4 cut(s) 236, 536, 797, 860
PsuI RGATCY 1 cut(s) 1117
PvuII CAGCTG 1 cut(s) 101
RsaI GTAC 2 cut(s) 119, 161
RsaNI GTAC 2 cut(s) 118, 160
RseI CAYNNNNRTG 2 cut(s) 980, 1005
SaqAI TTAA 3 cut(s) 437, 596, 810
SatI GCNGC 3 cut(s) 99, 258, 522
Sau3AI GATC 7 cut(s) 40, 72, 328, 364, 564, 615, 1117
Sau96I GGNCC 4 cut(s) 236, 536, 797, 860
ScaI AGTACT 1 cut(s) 119
SchI GAGTC 2 cut(s) 99, 578
SduI GDGCHC 2 cut(s) 1002, 1152
SfaNI GCATC 3 cut(s) 370, 774, 814
SfcI CTRYAG 2 cut(s) 357, 447
SinI GGWCC 4 cut(s) 236, 536, 797, 860
SmiMI CAYNNNNRTG 2 cut(s) 980, 1005
SmlI CTYRAG 3 cut(s) 15, 436, 1003
SmoI CTYRAG 3 cut(s) 15, 436, 1003
SphI GCATGC 1 cut(s) 341
Sse9I AATT 4 cut(s) 431, 597, 1044, 1069
SsiI CCGC 4 cut(s) 32, 257, 973, 993
SspMI CTAG 4 cut(s) 87, 680, 935, 957
StyI CCWWGG 3 cut(s) 141, 711, 1108
TaaI ACNGT 6 cut(s) 68, 164, 189, 576, 979, 1022
TaqI TCGA 2 cut(s) 477, 567
TasI AATT 4 cut(s) 431, 597, 1044, 1069
TatI WGTACW 2 cut(s) 117, 159
TauI GCSGC 1 cut(s) 260
Tru1I TTAA 3 cut(s) 437, 596, 810
Tru9I TTAA 3 cut(s) 437, 596, 810
TscAI CASTG 4 cut(s) 579, 654, 748, 1027
TseFI GTSAC 2 cut(s) 148, 570
TseI GCWGC 2 cut(s) 98, 521
Tsp45I GTSAC 2 cut(s) 148, 570
TspDTI ATGAA 2 cut(s) 578, 645
TspGWI ACGGA 1 cut(s) 873
TspRI CASTG 4 cut(s) 579, 654, 748, 1027
Van91I CCANNNNNTGG 2 cut(s) 852, 1114
Vha464I CTTAAG 1 cut(s) 436
VneI GTGCAC 1 cut(s) 1148
VpaK11BI GGWCC 4 cut(s) 236, 536, 797, 860
XapI RAATTY 2 cut(s) 431, 1069
XceI RCATGY 1 cut(s) 341
XspI CTAG 4 cut(s) 87, 680, 935, 957
ZrmI AGTACT 1 cut(s) 119
Zsp2I ATGCAT 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.