Rmu_sc0003262.1_g000014

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003262.1
OG0008827
Physical Location & Seq
Reverse (-)
41677 .. 46412
4736 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3465 bp
atgcttcacaggagcttcaagcccgccaaatgcaaaacggcgttgaagcttgccgtttcgagaataaagctgttgaagaataagaaagatgcccaggtgaagcagataaagagggaattggcccaattgctggagagtggtcagtacaggactgcaaggattcgggttgaacatgtggtgagggaagagaagttgagggcagcctatgaacttatcgagatatattgcgaacttgttgcagcacgattgcctatggttgaatcgcagaagaactgcctaattgatctgaaggaagcagtatcaagtgtaatttttgcatctcctagatgtgcggacgtaccagaactcatggatatccgcaagcacttcacagcaaaatacggaaaggagttcgtctctgcagctgttgaactgcggccggatagtggtgtaagccgcatgaatggaccaaatacgtttggaatggccagtatggattctgctccacctccacctactcatgatgtcaggggacctcctaatgtccaagttctgcctaatagcatggccagtaaagctcatgtggaacctccctctggtccacctccacctgatcaacctccaattgctcaagttccacgcagaaattatgagtatcacgatggacctatgaattctaatgagcagaatgcaagatcatcacctcacttggaggattctgattctacaaatgtttctgccaacaaagcaaccacatctgccaaatttcatccagaaacgacatcttcagaaaccagaagcagtcgaatggaaatgcatgtatttcctggggatgaaagttcttcgggcaggcagaattggaatatgcaatttaaggatgctgcatctgcagcacaggcagctgctgaatcagcagaactagcaggcatggcagccagagcagctgcagaactctcgaggcaatattcatctgaaatgcacaataaaagtaatcagtcagcctctgtgccaactaattcaagttccatgaacaatgaccatttggggaatagtctccagacagcagatcgatatcctcaaaagaagtcacctgaacgtcatgacattttagataaggcgagcatgaagatacagtctggtaattctgaggatatgcatgcaacggagatgcaaactgctatcaaacctgagaatattccttattttggagatataagatctgaaaagaaatctagcaggacttcctccccatctaattcaagtgttggtagcgatcatcaggaagacgttttgaaagaggatgatcatattaattactttgcagatctgggtactggaaaacaatctatcaggtcttcctctccttctcattcgggacattttagtaacaatcacgaaaatttcgctagtgtttctggtgagggttcttttgttagtgatgttaacatccatcaaagcacaattggaatgaattctgagggaaatgctcctctggtttttgatgattctggttcagacgatgataactacaagggacaagaatcaagtttgtttttttcttctcctgctagaaattcatttccttcagctagtttagatccttggagctttgagcaaaagagagatgaggtaccattgaagtccgtttcaccattacgttcttcatcagtgcagcactcccttcccattttttctgaaggtttgacaggttctactgtcccttcggaaccaaatgacttcgtgcctgttacttttgatgcttcagatggcccaagttcagacagtgaggaggagttggacacgtcgatgcttgtcctgagcaaagattctaaattttctcatgagaagactgttcaatccataaattcgggaaaaagccagagtgcaagccacaagtcattagcatcttcatcctctgatgaggtacatcctgagagaacccagggaattgaatatagtgttttctcagataaaaggtttgatgatgatgaattgcccggtagtgaaccatctccgaggctgaaaaaatctggattagatacaagtgccaaggatgatttacctcaaacagtgaaagacagtgaggaatgtagttgtgtaagtgatactgacaatgagttgttggcctttggggtcttgaaaggcggccttcggaacaagggttataggtatccaccatataatcggaatccatcaggcaatgcttttttgggcaaacaaattactcaagatggtccaacagtcaggacttccgatgtttctcatacgcctattgatgagccatatagccggaaaggaagcactaagctaaataaggaacaaagcactaaaaccccagtcacacatgtcactcctgatgatgattcttcagaggatgaactgtcacatcaaactctaagcagtagtcgccaagacttgtacaatcagaaatcggtctctcgagtagtacatgatgattcttcagaggatgaactctcacatgaaactcttagcaatagtcgccaagacctgtcccatcataaatcggtatcccaaggaaagacaagctcaacgtctgcatttttctttaattcagaggatagtgaggctgaggaagatgttcctagaaagatttccaccaccattgctcgtccaagttcaaaattttctagaaggacacaaccctcttcttcaaattctgttagaagttctagcataaggacaacagttgttcctgatgcaccaaggacttcagactatggaaaatcctcttcaagaagttccaatgacgctgaaactctaccagagccctcaagaagtcaggattggcagagacctatagaacaaacaagttcttattcaattccagaatccaagaagactgggacatcagtcaatggaaattcctcctcaagaagttcttatgccactgaaactctaccaagtgcttcatcccagaccaagcgctcagaccgtttggcaagtcaggagtggcgtagaccaatagaacaagcggcacctaaaccaataccagaatctaagaggtcctcgcgtgtggagacttcaaacttatttccaagggaggaatcatccaatactccaatgactgtaagatcagagagaactgaagctactgaaacttcaaagtcctcgggttcaaagtcatttggaagagaacaatcatctaatcctccaaagattgaaagatcagaaagcagcgaaagttcaaagtcctcgggttttaagtcatttgcaagagaccaatcgtccaatcttcctgcaaaggttgtaagatcacagagaagtgacccttcaaagttctctgctccgaagccatttacaagggaccaaacttccaatcctcctccaaagattgtaagatcagagagcgctgaaactccgaagtcctcttcttcagtggggaaggcttctcatgtccacccgaagcttcccgactctgatgacttgtttgccaagtttacagccctcaagcagaatcgacaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

1154

Amino Acids

126.42

Weight (kDa)

6.24

Isoelectric Point (pI)

65.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 12 - 146 1.9e-49 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1609
AccB1I GGYRCC 2 cut(s) 1609, 2974
AccB7I CCANNNNNTGG 1 cut(s) 130
AccI GTMKAC 1 cut(s) 2956
AccII CGCG 1 cut(s) 3010
AciI CCGC 7 cut(s) 24, 332, 358, 415, 436, 2124, 2972
AclWI GGATC 1 cut(s) 1571
AcoI YGGCCR 3 cut(s) 416, 465, 546
AfaI GTAC 7 cut(s) 146, 339, 1312, 1611, 1905, 2399, 2427
AfeI AGCGCT 2 cut(s) 2924, 3349
AfiI CCNNNNNNNGG 4 cut(s) 130, 425, 575, 1184
AflIII ACRYGT 3 cut(s) 172, 1779, 2323
AhdI GACNNNNNGTC 1 cut(s) 3223
AjiI CACGTC 1 cut(s) 1782
AjnI CCWGG 3 cut(s) 93, 805, 1920
AjuI GAANNNNNNNTTGG 2 cut(s) 2767, 2799
Alw26I GTCTC 6 cut(s) 400, 1037, 2419, 2785, 3011, 3210
AlwI GGATC 1 cut(s) 1571
AlwNI CAGNNNCTG 2 cut(s) 884, 983
Ama87I CYCGRG 4 cut(s) 934, 2418, 3109, 3193
Aor51HI AGCGCT 2 cut(s) 2924, 3349
AoxI GGCC 7 cut(s) 120, 416, 465, 546, 1747, 2103, 2125
AseI ATTAAT 1 cut(s) 1290
Asp700I GAANNNNTTC 3 cut(s) 1450, 2577, 2590
Asp718I GGTACC 1 cut(s) 1609
AspLEI GCGC 2 cut(s) 2925, 3350
AspS9I GGNCC 9 cut(s) 121, 446, 512, 578, 644, 1748, 2213, 3003, 3304
AsuC2I CCSGG 1 cut(s) 1977
AsuHPI GGTGA 6 cut(s) 109, 190, 672, 1059, 1409, 1620
AvaI CYCGRG 4 cut(s) 934, 2418, 3109, 3193
AvaII GGWCC 7 cut(s) 446, 512, 578, 644, 2213, 3003, 3304
BalI TGGCCA 2 cut(s) 467, 548
BanI GGYRCC 2 cut(s) 1609, 2974
BanII GRGCYC 1 cut(s) 2769
BbsI GAAGAC 4 cut(s) 1269, 1326, 1832, 2843
BbvCI CCTCAGC 1 cut(s) 2568
BccI CCATC 8 cut(s) 635, 1237, 1437, 1739, 1996, 2179, 2204, 2502
BceAI ACGGC 2 cut(s) 38, 54
BcgI CGANNNNNNTGC 4 cut(s) 218, 252, 915, 949
BciT130I CCWGG 3 cut(s) 95, 807, 1922
BciVI GTATCC 2 cut(s) 2160, 2518
BclI TGATCA 2 cut(s) 592, 1282
BcnI CCSGG 1 cut(s) 1977
BcoDI GTCTC 6 cut(s) 400, 1037, 2419, 2785, 3011, 3210
BfaI CTAG 9 cut(s) 324, 899, 1212, 1386, 1548, 1569, 2583, 2628, 2670
BfmI CTRYAG 4 cut(s) 399, 867, 924, 2796
BfoI RGCGCY 2 cut(s) 2926, 3351
BfuI GTATCC 2 cut(s) 2160, 2518
BglII AGATCT 2 cut(s) 1196, 1303
Bme1390I CCNGG 4 cut(s) 95, 807, 1922, 1977
Bme18I GGWCC 7 cut(s) 446, 512, 578, 644, 2213, 3003, 3304
BmeRI GACNNNNNGTC 1 cut(s) 3223
BmeT110I CYCGRG 4 cut(s) 934, 2418, 3109, 3193
BmgBI CACGTC 1 cut(s) 1782
BmgT120I GGNCC 9 cut(s) 121, 446, 512, 578, 644, 1748, 2213, 3003, 3304
BmiI GGNNCC 6 cut(s) 513, 567, 1611, 1707, 2976, 3305
BmrFI CCNGG 4 cut(s) 95, 807, 1922, 1977
BmrI ACTGGG 2 cut(s) 2309, 2850
BmsI GCATC 9 cut(s) 79, 326, 847, 872, 1137, 1726, 1776, 1892, 2686
BmuI ACTGGG 2 cut(s) 2309, 2850
BoxI GACNNNNGTC 1 cut(s) 2849
BpiI GAAGAC 4 cut(s) 1269, 1326, 1832, 2843
BplI GAGNNNNNCTC 2 cut(s) 380, 412
BpmI CTGGAG 2 cut(s) 152, 1019
Bpu10I CCTNAGC 2 cut(s) 1796, 2568
BpuEI CTTGAG 5 cut(s) 594, 2190, 2755, 2854, 3431
BpuMI CCSGG 1 cut(s) 1977
Bsa29I ATCGAT 1 cut(s) 1048
BsaBI GATNNNNATC 1 cut(s) 1050
BsaI GGTCTC 3 cut(s) 2419, 2785, 3210
Bsc4I CCNNNNNNNGG 4 cut(s) 130, 425, 575, 1184
Bse1I ACTGG 5 cut(s) 468, 549, 1318, 2315, 2845
Bse3DI GCAATG 2 cut(s) 2185, 2601
Bse8I GATNNNNATC 1 cut(s) 1050
BseBI CCWGG 3 cut(s) 95, 807, 1922
BseCI ATCGAT 1 cut(s) 1048
BseJI GATNNNNATC 1 cut(s) 1050
BseLI CCNNNNNNNGG 4 cut(s) 130, 425, 575, 1184
BseMI GCAATG 2 cut(s) 2185, 2601
BseMII CTCAG 8 cut(s) 1116, 1158, 1446, 1787, 1902, 1959, 2559, 2940
BseNI ACTGG 5 cut(s) 468, 549, 1318, 2315, 2845
BseRI GAGGAG 5 cut(s) 1458, 1781, 1784, 2857, 3312
BseX3I CGGCCG 1 cut(s) 416
BsgI GTGCAG 1 cut(s) 1670
Bsh1236I CGCG 1 cut(s) 3010
Bsh1285I CGRYCG 1 cut(s) 419
BshFI GGCC 7 cut(s) 122, 418, 467, 548, 1749, 2105, 2127
BshNI GGYRCC 2 cut(s) 1609, 2974
BshVI ATCGAT 1 cut(s) 1048
BsiEI CGRYCG 1 cut(s) 419
BsiHKCI CYCGRG 4 cut(s) 934, 2418, 3109, 3193
BsiSI CCGG 3 cut(s) 419, 1977, 2269
BslFI GGGAC 7 cut(s) 525, 1368, 1527, 1682, 2476, 2857, 3317
BslI CCNNNNNNNGG 4 cut(s) 130, 425, 575, 1184
BsmAI GTCTC 6 cut(s) 400, 1037, 2419, 2785, 3011, 3210
BsmBI CGTCTC 1 cut(s) 400
BsmFI GGGAC 7 cut(s) 525, 1368, 1527, 1682, 2476, 2857, 3317
BsmI GAATGC 1 cut(s) 673
BsnI GGCC 7 cut(s) 122, 418, 467, 548, 1749, 2105, 2127
Bso31I GGTCTC 3 cut(s) 2419, 2785, 3210
BsoBI CYCGRG 4 cut(s) 934, 2418, 3109, 3193
Bsp1286I GDGCHC 1 cut(s) 2769
Bsp1407I TGTACA 1 cut(s) 2397
BspACI CCGC 7 cut(s) 24, 332, 358, 415, 436, 2124, 2972
BspANI GGCC 7 cut(s) 122, 418, 467, 548, 1749, 2105, 2127
BspCNI CTCAG 8 cut(s) 1117, 1159, 1447, 1788, 1903, 1958, 2560, 2939
BspDI ATCGAT 1 cut(s) 1048
BspFNI CGCG 1 cut(s) 3010
BspHI TCATGA 3 cut(s) 499, 1078, 1819
BspLI GGNNCC 6 cut(s) 513, 567, 1611, 1707, 2976, 3305
BspMAI CTGCAG 3 cut(s) 403, 871, 928
BspPI GGATC 1 cut(s) 1571
BspT107I GGYRCC 2 cut(s) 1609, 2974
BspTNI GGTCTC 3 cut(s) 2419, 2785, 3210
BsrDI GCAATG 2 cut(s) 2185, 2601
BsrGI TGTACA 1 cut(s) 2397
BsrI ACTGG 5 cut(s) 468, 549, 1318, 2315, 2845
BssT1I CCWWGG 5 cut(s) 1580, 2028, 2512, 2702, 3035
Bst2UI CCWGG 3 cut(s) 95, 807, 1922
Bst6I CTCTTC 5 cut(s) 180, 2650, 2734, 3124, 3373
BstAUI TGTACA 1 cut(s) 2397
BstC8I GCNNGC 8 cut(s) 24, 51, 362, 830, 904, 1099, 1137, 1867
BstFNI CGCG 1 cut(s) 3010
BstH2I RGCGCY 2 cut(s) 2926, 3351
BstHHI GCGC 2 cut(s) 2925, 3350
BstMAI GTCTC 6 cut(s) 400, 1037, 2419, 2785, 3011, 3210
BstMCI CGRYCG 1 cut(s) 419
BstNI CCWGG 3 cut(s) 95, 807, 1922
BstNSI RCATGY 4 cut(s) 176, 800, 1139, 2327
BstPAI GACNNNNGTC 1 cut(s) 2849
BstSCI CCNGG 4 cut(s) 93, 805, 1920, 1975
BstSFI CTRYAG 4 cut(s) 399, 867, 924, 2796
BstUI CGCG 1 cut(s) 3010
BstV2I GAAGAC 4 cut(s) 1269, 1326, 1832, 2843
BstX2I RGATCY 3 cut(s) 1196, 1303, 1576
BstYI RGATCY 3 cut(s) 1196, 1303, 1576
BstZI CGGCCG 1 cut(s) 416
Bsu15I ATCGAT 1 cut(s) 1048
BsuI GTATCC 2 cut(s) 2160, 2518
BsuRI GGCC 7 cut(s) 122, 418, 467, 548, 1749, 2105, 2127
BsuTUI ATCGAT 1 cut(s) 1048
BtrI CACGTC 1 cut(s) 1782
BtsIMutI CAGTG 6 cut(s) 1653, 1768, 2055, 2065, 2886, 3381
Cac8I GCNNGC 8 cut(s) 24, 51, 362, 830, 904, 1099, 1137, 1867
CaiI CAGNNNCTG 2 cut(s) 884, 983
CciI TCATGA 3 cut(s) 499, 1078, 1819
CfoI GCGC 2 cut(s) 2925, 3350
Cfr13I GGNCC 9 cut(s) 121, 446, 512, 578, 644, 1748, 2213, 3003, 3304
ClaI ATCGAT 1 cut(s) 1048
CseI GACGC 1 cut(s) 2756
Csp6I GTAC 7 cut(s) 145, 338, 1311, 1610, 1904, 2398, 2426
CspCI CAANNNNNGTGG 2 cut(s) 2584, 2619
CviQI GTAC 7 cut(s) 145, 338, 1311, 1610, 1904, 2398, 2426
DriI GACNNNNNGTC 1 cut(s) 3223
EaeI YGGCCR 3 cut(s) 416, 465, 546
EagI CGGCCG 1 cut(s) 416
Eam1104I CTCTTC 5 cut(s) 180, 2650, 2734, 3124, 3373
Eam1105I GACNNNNNGTC 1 cut(s) 3223
EarI CTCTTC 5 cut(s) 180, 2650, 2734, 3124, 3373
EclXI CGGCCG 1 cut(s) 416
Eco130I CCWWGG 5 cut(s) 1580, 2028, 2512, 2702, 3035
Eco24I GRGCYC 1 cut(s) 2769
Eco31I GGTCTC 3 cut(s) 2419, 2785, 3210
Eco32I GATATC 2 cut(s) 355, 1052
Eco47I GGWCC 7 cut(s) 446, 512, 578, 644, 2213, 3003, 3304
Eco47III AGCGCT 2 cut(s) 2924, 3349
Eco52I CGGCCG 1 cut(s) 416
Eco88I CYCGRG 4 cut(s) 934, 2418, 3109, 3193
EcoO109I RGGNCCY 2 cut(s) 512, 3003
EcoRI GAATTC 2 cut(s) 652, 1450
EcoRII CCWGG 3 cut(s) 93, 805, 1920
EcoRV GATATC 2 cut(s) 355, 1052
EcoT14I CCWWGG 5 cut(s) 1580, 2028, 2512, 2702, 3035
EcoT22I ATGCAT 2 cut(s) 798, 1137
EcoT38I GRGCYC 1 cut(s) 2769
ErhI CCWWGG 5 cut(s) 1580, 2028, 2512, 2702, 3035
Esp3I CGTCTC 1 cut(s) 400
FalI AAGNNNNNCTT 6 cut(s) 2112, 2144, 2863, 2895, 3253, 3285
FaqI GGGAC 7 cut(s) 525, 1368, 1527, 1682, 2476, 2857, 3317
FauI CCCGC 1 cut(s) 31
FbaI TGATCA 2 cut(s) 592, 1282
FblI GTMKAC 1 cut(s) 2956
FriOI GRGCYC 1 cut(s) 2769
FspBI CTAG 9 cut(s) 324, 899, 1212, 1386, 1548, 1569, 2583, 2628, 2670
GlaI GCGC 2 cut(s) 2924, 3349
GsuI CTGGAG 2 cut(s) 152, 1019
HaeII RGCGCY 2 cut(s) 2926, 3351
HaeIII GGCC 7 cut(s) 122, 418, 467, 548, 1749, 2105, 2127
HapII CCGG 3 cut(s) 419, 1977, 2269
HgaI GACGC 1 cut(s) 2756
HhaI GCGC 2 cut(s) 2925, 3350
Hin6I GCGC 2 cut(s) 2923, 3348
HinP1I GCGC 2 cut(s) 2923, 3348
HincII GTYRAC 1 cut(s) 1423
HindII GTYRAC 1 cut(s) 1423
HindIII AAGCTT 2 cut(s) 47, 3404
HpaI GTTAAC 1 cut(s) 1423
HpaII CCGG 3 cut(s) 419, 1977, 2269
HphI GGTGA 6 cut(s) 109, 190, 672, 1059, 1409, 1620
Hpy166II GTNNAC 6 cut(s) 581, 1423, 1985, 2957, 3397, 3438
Hpy8I GTNNAC 6 cut(s) 581, 1423, 1985, 2957, 3397, 3438
Hpy99I CGWCG 1 cut(s) 1786
HpyCH4IV ACGT 7 cut(s) 336, 455, 1075, 1266, 1636, 1781, 2531
HpySE526I ACGT 7 cut(s) 336, 455, 1075, 1266, 1636, 1781, 2531
HspAI GCGC 2 cut(s) 2923, 3348
KpnI GGTACC 1 cut(s) 1613
Ksp22I TGATCA 2 cut(s) 592, 1282
KspAI GTTAAC 1 cut(s) 1423
LmnI GCTCC 5 cut(s) 12, 487, 1471, 1584, 3289
LweI GCATC 9 cut(s) 79, 326, 847, 872, 1137, 1726, 1776, 1892, 2686
MaeI CTAG 9 cut(s) 324, 899, 1212, 1386, 1548, 1569, 2583, 2628, 2670
MaeII ACGT 7 cut(s) 336, 455, 1075, 1266, 1636, 1781, 2531
MaeIII GTNAC 7 cut(s) 1065, 1364, 1726, 2317, 2326, 2361, 3263
MfeI CAATTG 3 cut(s) 125, 603, 1440
MflI RGATCY 3 cut(s) 1196, 1303, 1576
MhlI GDGCHC 1 cut(s) 2769
MlsI TGGCCA 2 cut(s) 467, 548
MluNI TGGCCA 2 cut(s) 467, 548
MlyI GAGTC 1 cut(s) 3407
MmeI TCCRAC 2 cut(s) 1755, 2240
Mox20I TGGCCA 2 cut(s) 467, 548
Mph1103I ATGCAT 2 cut(s) 798, 1137
MroXI GAANNNNTTC 3 cut(s) 1450, 2577, 2590
MscI TGGCCA 2 cut(s) 467, 548
MseI TTAA 5 cut(s) 852, 1290, 1422, 2547, 3201
MslI CAYNNNNRTG 1 cut(s) 1784
Msp20I TGGCCA 2 cut(s) 467, 548
MspA1I CMGCKG 3 cut(s) 404, 881, 923
MspI CCGG 3 cut(s) 419, 1977, 2269
MspR9I CCNGG 4 cut(s) 95, 807, 1922, 1977
MunI CAATTG 3 cut(s) 125, 603, 1440
Mva1269I GAATGC 1 cut(s) 673
MvaI CCWGG 3 cut(s) 95, 807, 1922
MvnI CGCG 1 cut(s) 3010
NciI CCSGG 1 cut(s) 1977
NlaIV GGNNCC 6 cut(s) 513, 567, 1611, 1707, 2976, 3305
NmuCI GTSAC 5 cut(s) 1065, 2317, 2326, 2361, 3263
NsiI ATGCAT 2 cut(s) 798, 1137
NspI RCATGY 4 cut(s) 176, 800, 1139, 2327
PaeI GCATGC 1 cut(s) 1139
PaeR7I CTCGAG 2 cut(s) 934, 2418
PagI TCATGA 3 cut(s) 499, 1078, 1819
PasI CCCWGGG 1 cut(s) 1921
PciI ACATGT 2 cut(s) 172, 2323
PctI GAATGC 1 cut(s) 673
PdmI GAANNNNTTC 3 cut(s) 1450, 2577, 2590
PflMI CCANNNNNTGG 1 cut(s) 130
PleI GAGTC 1 cut(s) 3407
PpsI GAGTC 1 cut(s) 3407
PpuMI RGGWCCY 2 cut(s) 512, 3003
PscI ACATGT 2 cut(s) 172, 2323
PshAI GACNNNNGTC 1 cut(s) 2849
PshBI ATTAAT 1 cut(s) 1290
Psp5II RGGWCCY 2 cut(s) 512, 3003
Psp6I CCWGG 3 cut(s) 93, 805, 1920
PspGI CCWGG 3 cut(s) 93, 805, 1920
PspN4I GGNNCC 6 cut(s) 513, 567, 1611, 1707, 2976, 3305
PspPI GGNCC 9 cut(s) 121, 446, 512, 578, 644, 1748, 2213, 3003, 3304
PspPPI RGGWCCY 2 cut(s) 512, 3003
PsrI GAACNNNNNNTAC 2 cut(s) 3073, 3105
PstI CTGCAG 3 cut(s) 403, 871, 928
PstNI CAGNNNCTG 2 cut(s) 884, 983
PsuI RGATCY 3 cut(s) 1196, 1303, 1576
PvuII CAGCTG 3 cut(s) 404, 881, 923
RsaI GTAC 7 cut(s) 146, 339, 1312, 1611, 1905, 2399, 2427
RsaNI GTAC 7 cut(s) 145, 338, 1311, 1610, 1904, 2398, 2426
RseI CAYNNNNRTG 1 cut(s) 1784
SaqAI TTAA 5 cut(s) 852, 1290, 1422, 2547, 3201
Sau96I GGNCC 9 cut(s) 121, 446, 512, 578, 644, 1748, 2213, 3003, 3304
SchI GAGTC 1 cut(s) 3407
ScrFI CCNGG 4 cut(s) 95, 807, 1922, 1977
SduI GDGCHC 1 cut(s) 2769
SfaNI GCATC 9 cut(s) 79, 326, 847, 872, 1137, 1726, 1776, 1892, 2686
SfcI CTRYAG 4 cut(s) 399, 867, 924, 2796
Sfr274I CTCGAG 2 cut(s) 934, 2418
SinI GGWCC 7 cut(s) 446, 512, 578, 644, 2213, 3003, 3304
SlaI CTCGAG 2 cut(s) 934, 2418
SmiMI CAYNNNNRTG 1 cut(s) 1784
SmlI CTYRAG 7 cut(s) 609, 934, 2205, 2418, 2770, 2869, 3446
SmoI CTYRAG 7 cut(s) 609, 934, 2205, 2418, 2770, 2869, 3446
SphI GCATGC 1 cut(s) 1139
SsiI CCGC 7 cut(s) 24, 332, 358, 415, 436, 2124, 2972
SspI AATATT 2 cut(s) 944, 1174
SspMI CTAG 9 cut(s) 324, 899, 1212, 1386, 1548, 1569, 2583, 2628, 2670
StyD4I CCNGG 4 cut(s) 93, 805, 1920, 1975
StyI CCWWGG 5 cut(s) 1580, 2028, 2512, 2702, 3035
TaiI ACGT 7 cut(s) 339, 458, 1078, 1269, 1639, 1784, 2534
TaqI TCGA 8 cut(s) 59, 216, 784, 935, 1048, 1784, 2419, 3457
TaqII GACCGA 1 cut(s) 2401
TatI WGTACW 3 cut(s) 144, 2397, 2425
TauI GCSGC 4 cut(s) 418, 438, 2127, 2975
Tru1I TTAA 5 cut(s) 852, 1290, 1422, 2547, 3201
Tru9I TTAA 5 cut(s) 852, 1290, 1422, 2547, 3201
TscAI CASTG 6 cut(s) 1653, 1768, 2055, 2065, 2893, 3381
TseFI GTSAC 5 cut(s) 1065, 2317, 2326, 2361, 3263
Tsp45I GTSAC 5 cut(s) 1065, 2317, 2326, 2361, 3263
TspGWI ACGGA 3 cut(s) 396, 1157, 1612
TspRI CASTG 6 cut(s) 1653, 1768, 2055, 2065, 2893, 3381
Van91I CCANNNNNTGG 1 cut(s) 130
VpaK11BI GGWCC 7 cut(s) 446, 512, 578, 644, 2213, 3003, 3304
VspI ATTAAT 1 cut(s) 1290
XbaI TCTAGA 1 cut(s) 2627
XceI RCATGY 4 cut(s) 176, 800, 1139, 2327
XhoI CTCGAG 2 cut(s) 934, 2418
XmiI GTMKAC 1 cut(s) 2956
XmnI GAANNNNTTC 3 cut(s) 1450, 2577, 2590
XspI CTAG 9 cut(s) 324, 899, 1212, 1386, 1548, 1569, 2583, 2628, 2670
Zsp2I ATGCAT 2 cut(s) 798, 1137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.