Rmu_sc0003316.1_g000010

Photosystem II (PSII) is a light-driven water plastoquinone oxidoreductase that uses light energy to abstract electrons from H(2)O, generating O(2) and a proton gradient subsequently used for ATP formation. It consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. The D1 D2 (PsbA PsbA) reaction center heterodimer binds P680, the primary electron donor of PSII as well as several subsequent electron acceptors

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003316.1
OG0005369
Physical Location & Seq
Forward (+)
36554 .. 37030
477 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 477 bp
atgactgcaattttagagagacgtgaaagcgaaagcctatggggtcgcttttgtaattggataaccagcactgaaaaccgtctttacattggatggtttggtgttttgatgatccctactttattgaccgcaacttctgtatttattattgctttcattgctgcacctccggtagatattgatggtattcgtgaacctgtttctggatctttactttacggaaacaatattatttctggtgccattatccctacttctgcagctataggtttgcacttttacccaatatgggaagcggcttccgttgatgaatggttatacaacggtggtccttatgagctaattgctctacacttcttacttggtgtagcttgctacatgggtcatgagtgggaacttagtttccgtctgggtatgcgcccttggattgctgttgcatattcagctcctgttgcagctgctactgctgttttctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
Photo_RC

Protein Analysis

158

Amino Acids

17.44

Weight (kDa)

4.87

Isoelectric Point (pI)

33.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Photo_RC PF00124 28 - 158 1.6e-37 Photosynthetic reaction centre protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 239
AciI CCGC 2 cut(s) 129, 296
AclWI GGATC 2 cut(s) 106, 214
AfiI CCNNNNNNNGG 2 cut(s) 203, 289
AjiI CACGTC 1 cut(s) 23
AloI GAACNNNNNNTCC 2 cut(s) 387, 419
AluBI AGCT 5 cut(s) 263, 340, 371, 446, 458
AluI AGCT 5 cut(s) 263, 340, 371, 446, 458
Alw26I GTCTC 1 cut(s) 13
AlwI GGATC 2 cut(s) 106, 214
AlwNI CAGNNNCTG 1 cut(s) 449
ApeKI GCWGC 4 cut(s) 161, 260, 455, 458
AspLEI GCGC 1 cut(s) 420
AspS9I GGNCC 1 cut(s) 329
AvaII GGWCC 1 cut(s) 329
BanI GGYRCC 1 cut(s) 239
BbvI GCAGC 4 cut(s) 148, 272, 445, 467
BccI CCATC 2 cut(s) 87, 176
BcoDI GTCTC 1 cut(s) 13
BfaI CTAG 1 cut(s) 475
BfmI CTRYAG 2 cut(s) 258, 264
BisI GCNGC 5 cut(s) 162, 261, 297, 456, 459
BlsI GCNGC 5 cut(s) 163, 262, 298, 457, 460
Bme18I GGWCC 1 cut(s) 329
BmgBI CACGTC 1 cut(s) 23
BmgT120I GGNCC 1 cut(s) 329
BmiI GGNNCC 1 cut(s) 241
BsaJI CCNNGG 1 cut(s) 422
BsaWI WCCGGW 1 cut(s) 169
Bsc4I CCNNNNNNNGG 2 cut(s) 203, 289
Bse3DI GCAATG 1 cut(s) 156
BseDI CCNNGG 1 cut(s) 422
BseGI GGATG 1 cut(s) 98
BseLI CCNNNNNNNGG 2 cut(s) 203, 289
BseMI GCAATG 1 cut(s) 156
BseXI GCAGC 4 cut(s) 148, 272, 445, 467
BsgI GTGCAG 1 cut(s) 147
BshNI GGYRCC 1 cut(s) 239
BsiSI CCGG 1 cut(s) 170
BslI CCNNNNNNNGG 2 cut(s) 203, 289
BsmAI GTCTC 1 cut(s) 13
BsmBI CGTCTC 1 cut(s) 13
Bsp143I GATC 2 cut(s) 111, 206
BspACI CCGC 2 cut(s) 129, 296
BspHI TCATGA 1 cut(s) 385
BspLI GGNNCC 1 cut(s) 241
BspMAI CTGCAG 1 cut(s) 262
BspPI GGATC 2 cut(s) 106, 214
BspT107I GGYRCC 1 cut(s) 239
BsrDI GCAATG 1 cut(s) 156
BssECI CCNNGG 1 cut(s) 422
BssMI GATC 2 cut(s) 111, 206
BssT1I CCWWGG 1 cut(s) 422
Bst4CI ACNGT 2 cut(s) 80, 326
BstC8I GCNNGC 1 cut(s) 373
BstDEI CTNAG 1 cut(s) 398
BstF5I GGATG 1 cut(s) 98
BstHHI GCGC 1 cut(s) 420
BstKTI GATC 2 cut(s) 114, 209
BstMAI GTCTC 1 cut(s) 13
BstMBI GATC 2 cut(s) 111, 206
BstMWI GCNNNNNNNGC 4 cut(s) 158, 443, 452, 464
BstSFI CTRYAG 2 cut(s) 258, 264
BstV1I GCAGC 4 cut(s) 148, 272, 445, 467
BstX2I RGATCY 1 cut(s) 206
BstYI RGATCY 1 cut(s) 206
BtrI CACGTC 1 cut(s) 23
BtsCI GGATG 1 cut(s) 98
BtsIMutI CAGTG 1 cut(s) 69
Cac8I GCNNGC 1 cut(s) 373
CaiI CAGNNNCTG 1 cut(s) 449
CciI TCATGA 1 cut(s) 385
CfoI GCGC 1 cut(s) 420
Cfr13I GGNCC 1 cut(s) 329
CviAII CATG 2 cut(s) 379, 386
CviJI RGCY 7 cut(s) 36, 263, 299, 340, 371, 446, 458
CviKI_1 RGCY 7 cut(s) 36, 263, 299, 340, 371, 446, 458
DdeI CTNAG 1 cut(s) 398
DpnI GATC 2 cut(s) 113, 208
DpnII GATC 2 cut(s) 111, 206
Eco130I CCWWGG 1 cut(s) 422
Eco47I GGWCC 1 cut(s) 329
EcoT14I CCWWGG 1 cut(s) 422
ErhI CCWWGG 1 cut(s) 422
Esp3I CGTCTC 1 cut(s) 13
FaeI CATG 2 cut(s) 382, 389
FaiI YATR 9 cut(s) 40, 266, 289, 319, 336, 380, 387, 416, 439
FatI CATG 2 cut(s) 378, 385
Fnu4HI GCNGC 5 cut(s) 162, 261, 297, 456, 459
FokI GGATG 1 cut(s) 105
Fsp4HI GCNGC 5 cut(s) 162, 261, 297, 456, 459
FspBI CTAG 1 cut(s) 475
GlaI GCGC 1 cut(s) 419
GluI GCNGC 5 cut(s) 162, 261, 297, 456, 459
HapII CCGG 1 cut(s) 170
HhaI GCGC 1 cut(s) 420
Hin1II CATG 2 cut(s) 382, 389
Hin6I GCGC 1 cut(s) 418
HinP1I GCGC 1 cut(s) 418
HpaII CCGG 1 cut(s) 170
Hpy166II GTNNAC 1 cut(s) 194
Hpy188III TCNNGA 3 cut(s) 191, 204, 386
Hpy8I GTNNAC 1 cut(s) 194
HpyCH4III ACNGT 2 cut(s) 80, 326
HpyCH4IV ACGT 1 cut(s) 22
HpyCH4V TGCA 6 cut(s) 8, 164, 260, 274, 437, 455
HpyF10VI GCNNNNNNNGC 4 cut(s) 158, 443, 452, 464
HpyF3I CTNAG 1 cut(s) 398
HpySE526I ACGT 1 cut(s) 22
Hsp92II CATG 2 cut(s) 382, 389
HspAI GCGC 1 cut(s) 418
Kzo9I GATC 2 cut(s) 111, 206
LmnI GCTCC 1 cut(s) 451
LpnPI CCDG 7 cut(s) 79, 183, 189, 210, 222, 395, 462
Lsp1109I GCAGC 4 cut(s) 148, 272, 445, 467
MaeI CTAG 1 cut(s) 475
MaeII ACGT 1 cut(s) 22
MalI GATC 2 cut(s) 113, 208
MboI GATC 2 cut(s) 111, 206
MflI RGATCY 1 cut(s) 206
MluCI AATT 3 cut(s) 9, 55, 342
MnlI CCTC 1 cut(s) 177
MspA1I CMGCKG 1 cut(s) 458
MspI CCGG 1 cut(s) 170
MwoI GCNNNNNNNGC 4 cut(s) 158, 443, 452, 464
NdeII GATC 2 cut(s) 111, 206
NlaIII CATG 2 cut(s) 382, 389
NlaIV GGNNCC 1 cut(s) 241
PagI TCATGA 1 cut(s) 385
PkrI GCNGC 5 cut(s) 163, 262, 298, 457, 460
PspN4I GGNNCC 1 cut(s) 241
PspPI GGNCC 1 cut(s) 329
PstI CTGCAG 1 cut(s) 262
PstNI CAGNNNCTG 1 cut(s) 449
PsuI RGATCY 1 cut(s) 206
PvuII CAGCTG 1 cut(s) 458
SatI GCNGC 5 cut(s) 162, 261, 297, 456, 459
Sau3AI GATC 2 cut(s) 111, 206
Sau96I GGNCC 1 cut(s) 329
SetI ASST 9 cut(s) 25, 169, 199, 265, 271, 342, 373, 448, 460
SfcI CTRYAG 2 cut(s) 258, 264
SinI GGWCC 1 cut(s) 329
Sse9I AATT 3 cut(s) 9, 55, 342
SsiI CCGC 2 cut(s) 129, 296
SspI AATATT 1 cut(s) 229
SspMI CTAG 1 cut(s) 475
StyI CCWWGG 1 cut(s) 422
TaaI ACNGT 2 cut(s) 80, 326
TaiI ACGT 1 cut(s) 25
TasI AATT 3 cut(s) 9, 55, 342
TauI GCSGC 1 cut(s) 299
TscAI CASTG 1 cut(s) 76
TseI GCWGC 4 cut(s) 161, 260, 455, 458
TspDTI ATGAA 2 cut(s) 145, 324
TspGWI ACGGA 3 cut(s) 234, 292, 395
TspRI CASTG 1 cut(s) 76
VpaK11BI GGWCC 1 cut(s) 329
XspI CTAG 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.