Rmu_sc0003735.1_g000004
MADS Family

MADS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003735.1
OG0011525
Physical Location & Seq
Forward (+)
10978 .. 11796
819 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 819 bp
atggtgacgaaatcccagaccaccatatccggagaaaacatcaaagagatcagaagtctgagatttttggaaaaaccaattcagaagagaaagtccactctgaagaagagcgcgagccagctcgagattctctgtggtgcgcaggtttgctgcgttttggtggggccaactgggaatgttgaggtctggccggaaaacccagcagacgccgtatccatcatcgccaagtacaaacaatgcaaaaagaaaacaaaggtctgtagggaggtcaaccgatccaagaacgtggagcagtttgaagaaaacaagggtttggctatgggtgatgatgacattgatcaatccatgaataccctggagaactatgaggaagacaaggggtttgatattgatcaatttctgaataacactatggagaacttagaagatcaaaacaagggtatcgatatgggtgatgatattgatcaatttctcatgaataacactagggaaaatgttgaaccaaacaacggctttgacaccgatcatgattttgatcaatttctgaatgaattttttggtgatactaatgatcttaaagtgggctttccagagtgttcaaccagcaatgctcacatgccaacaccagggtttgaggatcatcagattgataatgtatgtggagtgtggccgtgggagacattatggtccacgccacaatactcagggttcgacggagcatactacctgccgaaatttgagaatgatggctttctttgtcaagagcagccaaaattgatgggttctaaccaaacttttcgagatattggaagcaattaa

Protein Analysis

272

Amino Acids

30.92

Weight (kDa)

4.51

Isoelectric Point (pI)

30.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 28 - 63 1.4e-09 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 685
Acc16I TGCGCA 1 cut(s) 141
Acc36I ACCTGC 2 cut(s) 133, 735
AccII CGCG 1 cut(s) 113
AccIII TCCGGA 1 cut(s) 29
AclWI GGATC 2 cut(s) 270, 645
AcoI YGGCCR 2 cut(s) 188, 668
AcsI RAATTY 2 cut(s) 551, 734
AcuI CTGAAG 1 cut(s) 122
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 1 cut(s) 230
AfiI CCNNNNNNNGG 2 cut(s) 509, 626
AgsI TTSAA 3 cut(s) 299, 500, 600
AjnI CCWGG 2 cut(s) 354, 625
AluBI AGCT 1 cut(s) 121
AluI AGCT 1 cut(s) 121
Alw26I GTCTC 1 cut(s) 671
AlwI GGATC 2 cut(s) 270, 645
Ama87I CYCGRG 1 cut(s) 122
Aor13HI TCCGGA 1 cut(s) 29
AoxI GGCC 3 cut(s) 164, 188, 668
ApeKI GCWGC 2 cut(s) 150, 766
ApoI RAATTY 2 cut(s) 551, 734
ArsI GACNNNNNNTTYG 2 cut(s) 365, 397
AspLEI GCGC 2 cut(s) 113, 142
AspS9I GGNCC 2 cut(s) 164, 687
AsuHPI GGTGA 4 cut(s) 16, 335, 464, 572
AvaI CYCGRG 1 cut(s) 122
AvaII GGWCC 1 cut(s) 687
BaeI ACNNNNGTAYC 2 cut(s) 424, 457
BbsI GAAGAC 1 cut(s) 378
BbvI GCAGC 2 cut(s) 137, 778
BccI CCATC 3 cut(s) 224, 740, 772
BceAI ACGGC 3 cut(s) 194, 526, 655
BciT130I CCWGG 2 cut(s) 356, 627
BciVI GTATCC 1 cut(s) 223
BclI TGATCA 4 cut(s) 337, 391, 463, 535
BcoDI GTCTC 1 cut(s) 671
BfaI CTAG 1 cut(s) 486
BfmI CTRYAG 1 cut(s) 259
BfuAI ACCTGC 2 cut(s) 133, 735
BfuI GTATCC 1 cut(s) 223
BisI GCNGC 2 cut(s) 151, 767
BlsI GCNGC 2 cut(s) 152, 768
Bme1390I CCNGG 2 cut(s) 356, 627
Bme18I GGWCC 1 cut(s) 687
BmeT110I CYCGRG 1 cut(s) 122
BmgT120I GGNCC 2 cut(s) 164, 687
BmiI GGNNCC 1 cut(s) 165
BmrFI CCNGG 2 cut(s) 356, 627
BmrI ACTGGG 1 cut(s) 180
BmuI ACTGGG 1 cut(s) 180
BpiI GAAGAC 1 cut(s) 378
BpmI CTGGAG 1 cut(s) 377
Bsa29I ATCGAT 1 cut(s) 444
BsaBI GATNNNNATC 3 cut(s) 390, 462, 534
BsaHI GRCGYC 1 cut(s) 207
BsaJI CCNNGG 3 cut(s) 354, 626, 671
BsaWI WCCGGW 1 cut(s) 29
Bsc4I CCNNNNNNNGG 2 cut(s) 509, 626
Bse1I ACTGG 1 cut(s) 175
Bse3DI GCAATG 1 cut(s) 613
Bse8I GATNNNNATC 3 cut(s) 390, 462, 534
BseAI TCCGGA 1 cut(s) 29
BseBI CCWGG 2 cut(s) 356, 627
BseCI ATCGAT 1 cut(s) 444
BseDI CCNNGG 3 cut(s) 354, 626, 671
BseJI GATNNNNATC 3 cut(s) 390, 462, 534
BseLI CCNNNNNNNGG 2 cut(s) 509, 626
BseMI GCAATG 1 cut(s) 613
BseMII CTCAG 2 cut(s) 50, 717
BseNI ACTGG 1 cut(s) 175
BseXI GCAGC 2 cut(s) 137, 778
BseYI CCCAGC 1 cut(s) 199
Bsh1236I CGCG 1 cut(s) 113
BshFI GGCC 3 cut(s) 166, 190, 670
BshVI ATCGAT 1 cut(s) 444
BsiHKCI CYCGRG 1 cut(s) 122
BsiSI CCGG 2 cut(s) 30, 191
BslI CCNNNNNNNGG 2 cut(s) 509, 626
BsmAI GTCTC 1 cut(s) 671
BsnI GGCC 3 cut(s) 166, 190, 670
BsoBI CYCGRG 1 cut(s) 122
Bsp13I TCCGGA 1 cut(s) 29
BspANI GGCC 3 cut(s) 166, 190, 670
BspCNI CTCAG 2 cut(s) 51, 716
BspDI ATCGAT 1 cut(s) 444
BspEI TCCGGA 1 cut(s) 29
BspFNI CGCG 1 cut(s) 113
BspHI TCATGA 2 cut(s) 474, 526
BspLI GGNNCC 1 cut(s) 165
BspMI ACCTGC 2 cut(s) 133, 735
BspPI GGATC 2 cut(s) 270, 645
BspQI GCTCTTC 1 cut(s) 101
BsrDI GCAATG 1 cut(s) 613
BsrI ACTGG 1 cut(s) 175
BssECI CCNNGG 3 cut(s) 354, 626, 671
BssNI GRCGYC 1 cut(s) 207
Bst2UI CCWGG 2 cut(s) 356, 627
Bst6I CTCTTC 2 cut(s) 80, 101
BstACI GRCGYC 1 cut(s) 207
BstC8I GCNNGC 2 cut(s) 115, 119
BstDEI CTNAG 3 cut(s) 59, 421, 703
BstDSI CCRYGG 1 cut(s) 671
BstFNI CGCG 1 cut(s) 113
BstHHI GCGC 2 cut(s) 113, 142
BstMAI GTCTC 1 cut(s) 671
BstNI CCWGG 2 cut(s) 356, 627
BstNSI RCATGY 1 cut(s) 619
BstSCI CCNGG 2 cut(s) 354, 625
BstSFI CTRYAG 1 cut(s) 259
BstUI CGCG 1 cut(s) 113
BstV1I GCAGC 2 cut(s) 137, 778
BstV2I GAAGAC 1 cut(s) 378
BstXI CCANNNNNNTGG 1 cut(s) 286
Bsu15I ATCGAT 1 cut(s) 444
BsuI GTATCC 1 cut(s) 223
BsuRI GGCC 3 cut(s) 166, 190, 670
BsuTUI ATCGAT 1 cut(s) 444
BtgI CCRYGG 1 cut(s) 671
BtgZI GCGATG 1 cut(s) 205
BveI ACCTGC 2 cut(s) 133, 735
Cac8I GCNNGC 2 cut(s) 115, 119
CciI TCATGA 2 cut(s) 474, 526
CfoI GCGC 2 cut(s) 113, 142
Cfr13I GGNCC 2 cut(s) 164, 687
ClaI ATCGAT 1 cut(s) 444
CseI GACGC 1 cut(s) 215
Csp6I GTAC 1 cut(s) 229
CviAII CATG 4 cut(s) 346, 475, 527, 616
CviQI GTAC 1 cut(s) 229
DdeI CTNAG 3 cut(s) 59, 421, 703
DrdI GACNNNNNNGTC 1 cut(s) 685
DseDI GACNNNNNNGTC 1 cut(s) 685
EaeI YGGCCR 2 cut(s) 188, 668
Eam1104I CTCTTC 2 cut(s) 80, 101
EarI CTCTTC 2 cut(s) 80, 101
Eco47I GGWCC 1 cut(s) 687
Eco57I CTGAAG 1 cut(s) 122
Eco88I CYCGRG 1 cut(s) 122
EcoRII CCWGG 2 cut(s) 354, 625
FaeI CATG 4 cut(s) 349, 478, 530, 619
FatI CATG 4 cut(s) 345, 474, 526, 615
FbaI TGATCA 4 cut(s) 337, 391, 463, 535
Fnu4HI GCNGC 2 cut(s) 151, 767
Fsp4HI GCNGC 2 cut(s) 151, 767
FspBI CTAG 1 cut(s) 486
FspI TGCGCA 1 cut(s) 141
GlaI GCGC 2 cut(s) 112, 141
GluI GCNGC 2 cut(s) 151, 767
GsaI CCCAGC 1 cut(s) 203
GsuI CTGGAG 1 cut(s) 377
HaeIII GGCC 3 cut(s) 166, 190, 670
HapII CCGG 2 cut(s) 30, 191
HgaI GACGC 1 cut(s) 215
HhaI GCGC 2 cut(s) 113, 142
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 4 cut(s) 349, 478, 530, 619
Hin6I GCGC 2 cut(s) 111, 140
HinP1I GCGC 2 cut(s) 111, 140
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HinfI GANTC 1 cut(s) 127
HpaII CCGG 2 cut(s) 30, 191
HphI GGTGA 4 cut(s) 16, 335, 464, 572
Hpy166II GTNNAC 3 cut(s) 96, 271, 690
Hpy188I TCNGA 7 cut(s) 53, 60, 84, 102, 402, 546, 645
Hpy188III TCNNGA 7 cut(s) 30, 124, 475, 527, 590, 761, 800
Hpy8I GTNNAC 3 cut(s) 96, 271, 690
Hpy99I CGWCG 1 cut(s) 716
HpyCH4IV ACGT 1 cut(s) 285
HpyCH4V TGCA 1 cut(s) 240
HpyF3I CTNAG 3 cut(s) 59, 421, 703
HpySE526I ACGT 1 cut(s) 285
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 4 cut(s) 349, 478, 530, 619
HspAI GCGC 2 cut(s) 111, 140
Kpn2I TCCGGA 1 cut(s) 29
Ksp22I TGATCA 4 cut(s) 337, 391, 463, 535
LguI GCTCTTC 1 cut(s) 101
LmnI GCTCC 2 cut(s) 289, 716
Lsp1109I GCAGC 2 cut(s) 137, 778
MaeI CTAG 1 cut(s) 486
MaeII ACGT 1 cut(s) 285
MaeIII GTNAC 1 cut(s) 4
MboII GAAGA 6 cut(s) 97, 115, 118, 311, 383, 437
MluCI AATT 8 cut(s) 78, 395, 467, 539, 551, 734, 773, 814
MnlI CCTC 4 cut(s) 175, 259, 361, 628
MroI TCCGGA 1 cut(s) 29
MseI TTAA 2 cut(s) 576, 817
MspI CCGG 2 cut(s) 30, 191
MspR9I CCNGG 2 cut(s) 356, 627
MvaI CCWGG 2 cut(s) 356, 627
MvnI CGCG 1 cut(s) 113
NlaIII CATG 4 cut(s) 349, 478, 530, 619
NlaIV GGNNCC 1 cut(s) 165
NmuCI GTSAC 1 cut(s) 4
NsbI TGCGCA 1 cut(s) 141
NspI RCATGY 1 cut(s) 619
PaeR7I CTCGAG 1 cut(s) 122
PagI TCATGA 2 cut(s) 474, 526
PciSI GCTCTTC 1 cut(s) 101
PfeI GAWTC 1 cut(s) 127
PkrI GCNGC 2 cut(s) 152, 768
Psp6I CCWGG 2 cut(s) 354, 625
PspFI CCCAGC 1 cut(s) 199
PspGI CCWGG 2 cut(s) 354, 625
PspN4I GGNNCC 1 cut(s) 165
PspPI GGNCC 2 cut(s) 164, 687
RsaI GTAC 1 cut(s) 230
RsaNI GTAC 1 cut(s) 229
SapI GCTCTTC 1 cut(s) 101
SaqAI TTAA 2 cut(s) 576, 817
SatI GCNGC 2 cut(s) 151, 767
Sau96I GGNCC 2 cut(s) 164, 687
ScrFI CCNGG 2 cut(s) 356, 627
SetI ASST 7 cut(s) 123, 147, 186, 258, 270, 288, 729
SfcI CTRYAG 1 cut(s) 259
Sfr274I CTCGAG 1 cut(s) 122
SinI GGWCC 1 cut(s) 687
SlaI CTCGAG 1 cut(s) 122
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 8 cut(s) 78, 395, 467, 539, 551, 734, 773, 814
SspMI CTAG 1 cut(s) 486
StyD4I CCNGG 2 cut(s) 354, 625
TaiI ACGT 1 cut(s) 288
TaqI TCGA 4 cut(s) 123, 444, 711, 799
TasI AATT 8 cut(s) 78, 395, 467, 539, 551, 734, 773, 814
TatI WGTACW 1 cut(s) 228
TfiI GAWTC 1 cut(s) 127
Tru1I TTAA 2 cut(s) 576, 817
Tru9I TTAA 2 cut(s) 576, 817
TseFI GTSAC 1 cut(s) 4
TseI GCWGC 2 cut(s) 150, 766
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 3 cut(s) 362, 491, 564
TspGWI ACGGA 1 cut(s) 729
VpaK11BI GGWCC 1 cut(s) 687
XapI RAATTY 2 cut(s) 551, 734
XceI RCATGY 1 cut(s) 619
XcmI CCANNNNNNNNNTGG 1 cut(s) 352
XhoI CTCGAG 1 cut(s) 122
XspI CTAG 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.