Rmu_sc0003736.1_g000001

RNA processing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003736.1
OG0005246
Physical Location & Seq
Reverse (-)
757 .. 15421
14665 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 9165 bp
atggagcccgacggcgcagcgccgatgcaggtcgagtccgcggcggacaaaagccctaactcggagctccctcccaaatccagagaagaaggcgagctctcttcggatgataatgacgaaaatcctgttctctcggttgcacgttccactggtactacagggcccatgctggttccaccagtgaacaaattcacccatgggaatcaagcggggaaggctgtatctcctgcaagctctgctgatattcagtgccaaacctccaagcaatctacttcccagaagagcaatgatgcgaacagagtaccgttaaaacctgctacacctggatggcgtcctccacgtgcccactcggggcccaacaataatcttgtgataagcttctcagatgatgacagtcagagtgattctgaagaaaaagagcgtgggaaactaaaagctttacaaactaaaagtaatatggctcgagggaatgctaatggaaaaccacccatttcttcacttgcaaaaccaaacaagttgggacagcctgccagaaatgtgaacaaagtaatgcccaaaaagttgtctatgaatcgcacatttatgacatcaatggcgaatatccgtggagttaattctagagattctgtgccttcatcagttgagcaaggatctcgagctggaaatttcaattccatgaacaaaaatatagtgaacagagagcgtggatatgaacttcaagacttgcgacagcagattgcacttaaggagactgaactgaagctcaaggaaagtgaactgaagcttaagtctgcccagcgaactaaggaatcagttacatgcaaggatgagaatgctaagggcctgcacagagatggacctggtaagtacagtacaggatattctgatggtatgcagacagaaccaaaagagccagataaaaagcgtataaaagtcagcggtgctttttcgactcggctaactacactaagcccacaagaattacctgttgcaaaacctttattaccgtcaaaaaccccagcaatggaggacaatactcaattgaacaccagtaagattgatcatctccaaaaagaaaatcaagtgcgtccaacagagtcgagcattgtaaaatggcaagatcctaatgataaacatgttgctggaatgttgggaaatatacatactggactgaaagaaggtgctggcatcaatgctaaatatatccagtccgatgggaggggtaagcagatggattctgttaaccaggctaaatcactggcaaatatgacgtgcattgattttccaaataacttgaatagtgtggagttgaatcatacaaatactaacacaaacacaaatagcggccatccagaacctggatccttcttcaacaatccaacatcaggaaagactttaatgggaagtggtgaccatcaggaaaccatatccattgacaagagacttgaaccctcattctacagtaaatgtcaggcactcctaaacaacacaaacctctcgaactgttttggtaatgcaaacgttacaggagatgacaacatgaagatgcagtcattggttcaaatggaggaaatgctagacaagaatctggaggaagcacaagagcataggcgaaggtgtgaaattgaagaacaaaatgctcttaaagcttatcgtaaagcgcagagggctcttcttgaggcgaatgctaggtgtgatgttctttatcggaaaagggaactatattctgctgacttccgtgcttacgttattaacaatcccagtttgttatgttcctcaaggcagaatgaacaagctggacttgggttggatcaccctaataacttgtctgaaaatgtgaatttgatacccacgtcaagccatcagatgccgccggaccagaatgactgcaatctagcggttattgattccaacatccaatgtgttaacagctctcagatcccgaatccctaccatcatttgagtggagaaaacattggctcagaaccatgcagtgaacctgacgctagtacatctgagccagtgcctcttctgggcaatactggcacagatggagtttgctctccatccaatgaaccaaatggttcagcaaatgaagatgatgatacattttcattcgaaaatgaatctgttcaacccaatggtgaatgccatcttgtagacaaacaaaaggagacagataatgaatcaaataggataatgtctattgaccccaacgaggaatctttacttctcgaaaaagctttgaggtctacactatttgcaaagctaggaacaaaaaatgtgttgaagaacagtgttgtatgtaatggcacagggattgcagtggaacgagaggctgaaaatgatgcgagaagtgaagaaccacaaaaagtaaacggaagttctcccgtctcagaggtggagaaaaatcaacaatctgatattgagggcgttgaagtgcatgaaaaaagtttcactgaggctcctcttgagatccagagaaagcattccatcgaacatttgtctttgaattctcattccagtggatattttgaggacagatgttcctttggaggtgaccactcattgacttctatgatcttttcgccttcctacatcttgagtagtgctcttgggtatatgaaggttatagacccaaacaatgtgatggaacatcaggacagaagtcaacagagtggaactcgtgatactaatattgaagaaggtgcttgtataaactctggtaaagtgcagtttagcagtaccatggtagacacaacgaaaaaggctctggtgaaattatgtgatagagaagatggctcttataccactggtcctgctgttgatcccttttggccactttgcatgtatgagctacgaggaaaatgcaacaatgatgagtgtccttggcagcatgttaaggactactctaccaacgatatgtctccacatcagcatgatcattctgaaattgctggttgtcaggttggacaaacgttttgcaaagaaaagtgtgatggttcagcaaaagttccctggcgtcacaattcacggagaaatggtgaatgctggaagaagtgtttcagccttttcttagcattgtcaaagtcgtttcaaaaagattttcctacagatggaccggtctcgcatggaaatgacggccgcatcgaggtccctgtgagttggaacagacagtcatcgtatttccagagtagaaatagcggagtgaatcaaccggatcaagctttggctgacaatgagcaatacctggaaaaggctcttttaattttcagtcaggaggtcaatgcacttgagggtatgaaaaaggctcttcctgtattggcacgtgctcttgaggctgatccaacatccgtagttctctgggtcttttatctacttatttactatagcaatatgaagtcagtagggaaggatgacatgttctcttgtgcggtcaactacaatgatcaatcttatgaactttggattatgttcatcaacagtcgggtgcggctaagtgaccgattggttacctacgatcttgccctctcagcactgtgccgccatgcttcttctgccaaagataaagtgcatgctagtgcttgtatcttagacttagttttgcaaatggtggactgtctatgcatgtctgggaatgttgagagggccattcagaaaatttgtggactcttctctgctgccacaaatatttatgaccccgattctccattgcttactgatatgcccaaatgcctaacaagtcatgacaaatgtattttgggggtgtgttgtgtttacttggttatctataggaaacttcctgatgctgtagtactgcagtttgaatgtcagaaagaactgtttgcaatagaatggccttctattgaattaacagataatgaaaagcagagggctgtcaaactgatggaagcagtagaagattctgtatgtcaatcgctggataaaggtgattttaatctcagtttggcacatttttttgctctgagccacttaaggtgcatggcagctattgacagcttagaaaggtgtagtaatcttctgggtaaatatcttaaaatgttcccgtcctgcttagagcttgttctaatatcggcaagggcccaaaaacatgctcctggtgattcgctttttgacgggtttgaggaagccctaaataattggccaaaagaagtcccgggaattcaatgcatctggaatcagtatgttgtatatgcctttcagaaaggacaatttgattttggtaaagaacttataggccgttggtttcattctttttggcaagttcattgtattcgaaatggtacattagatgacatggagtgtgataactcagatggctcactaggattggcttcggattcatttctgcagacacttaatactgattctaagcagatggacgtaatgtttggatatcttaatctctcgctgtataagttaatccaaaatgatcagattgaagctcgcttagccctcgagagggcattgaaggctgctgttccagaatattcaaagcattgcatgagggagcatgcattatttatgctaagtgaggaatcaggatttaaggagaatggttgtcctagtggcatggagaagattttgaaacgttacataggtgatgccgaagttttccgttcatctgagccattatctaggcagtttattgccaacatcaagaagccaagagtacgacagctggttagtaacgcattcagtccattctcatctgattttgctcttgtaaattcagttcttgaagtgttgtatggaccttctcttttaccgaaaaagactggtgagacaaaatgtctggtggattttgttgaagctatcttggatataactccatccaactatcagttagctatttctgtctgtaagctgttaattactggcaacaatgtgactgatattacttcagtcagtgttttgttctgggctagctcaaacttggtcagtgcaatttttcgtgctgtcccaattccgccagagtatgtatgggtagaagctgcagacatattgggcaatatggtgaatgttgaggttatttctgagaggttttataagagagctttatctgtatatcctttctctgtcaagttgtggaaatcgtattacatgctttctatgatgactacaggaaatatgaacactgttgtggaaacagcaaaggaaaaagggcacagatttagcgccatgaataatctgaaactctactcggaggtctctctgaacctccagttacagtccccacaagaagagctcatcctcttctcggccttcgacatcgagcagagccgcctcttcttcgcctcctccgccaacaacatctacgccacccacctctcctctctccagcacgagagggcgtggagcaagacctcgattcccgcgcaggtcactcggatcgagctggatgaggccgaggactccatcacttcgtttgtttacctcatggagaaggaggcactcttggttgggaccagtaaaggccttctgctgctgcatagtgtggatgacaatgcctcgcaagtcgtcggcggcgtcgacggcggcgtgaggtgtgtttcggctagtccggatggagatgttctggctataattactgggactgggcagatactggtgatgactctggattgggacttgctttacgagactgcacttgaggatgttgctgaagatggcagccgcatatgtgatctaacattgtcgaatattgaaagccccattgcttggcgcggtgatgggaaatactttgtcacactaagcgaggcgttggattctacctctatgcttaagaggcttaaggtttgggagcgacattcaggggcgttacatgctgttacggaatcaaagtcgttcatgggttcagtggtggactggatgccaagtggggcgaaagttgcagctgtttatgataggaaagcgcaaaatgagtgtccttcaatagtgttttacgagaggaatgggttagaaagaagcctgtttagcattaatgagcaagctaatgcaactgtagaatttctcaagtggaattgcagttcagatcttcttgccgccgttgtcagatgtgataactatgactgtgtcaagatttggtatttcagtaacaatcactggtacttaaagtctgaaattagatacccaagacacgacggagtgaggtttgtctggaatccaacaaggccactgcagttaatatgttggactcttggcggccagattacatcttacaacttcatatggaattcagccgtgatggaggactcaactgcattggtagttgatgactccaagatacttgtaacccccctttctttatacttaatgccacctcctatgtatttattcagccttaaattctcgagtgttgtccgggactttgccttctactccaagaattctaagaactgcttggctgcttttctatcagatggctgtttatgtattgtagagcttcctgcaactgatacatgggaagatctagaaggaaaagagttacttgttgaagcgtccagttctgattcaccatttggttcagttttgcatctaatatggttggatccccataagattcttgctgtttctcaccatgggttcagtcatagtaattacttgcctcagacttcacttggtgaggaagaccttggtttttatttgcaggaaattgagcttacatgctctgaggatcatgtcccaggtttattgacatgctcaggttggaatgctaaagtctccagtcgaaactctctagaagagaccattattggaattactcctaatcctgcttcaaaaggttcagcatttgttcaatttgatggtggaaaggtttatgagtatgttccaaagttaggcatctcccgaggggcttcaaagcttgattggagcttctcatcaacttgcccttggatgagtgcggttctagttggagacagtgtgtcatcgaaacctctgcttttcggacttgatgacagttgtaggctgcacgtcagtggaaagataatatgcaacaactgcagcagtttttcattctactcaaatttggctgatcaggtgatcacacatttaattttggcaactaagcaggatttgctttttgttgttgaaatatcagatgtattgcaaaaagaacttgagataaaacatgagaacttcatccatgctggtaagaagaaaagagaagaaaatagaaactttataaacatatgggaaagaggtgccaaagttgttggtgtcgttcatggagatgaagccgctgtcctgttgcaaccatctcgtggcaatctagaatgcatttacccaagaaagttggtcctggcctctatttgcaatgcattggtccaaaggcgttttagagatgcacttctcatggtgaggcgtcagaggatagatttcaatgtcttggttgactattgtggttggcaggtgtttcttcaatcagcggcagaatttgtcaagcaggtcaataatttgaaccacatgactgagtttgtttgtgccataaagaatgaagatactacagagacgctatataaggaatttatttcttcaccctctcctaaagaagctaaagatgtgcaatcacatgactccaagggttttgattccaataacaaggtatcttctgtgctgctggccattaggaaggctcttgaggatcaattaccggaaactcctgcaagggagctttgcattctgaccactcttgctcgtagtgacccccctgcacttgatgaggctttagagagaataaaagttatccgtgaggcagaattgtcaggttccaatgaccaaaggagaatgtcttaccccactgctgaagaggctttgaagcatctcctatggttatctgattcggaatctgtttatgaggcagctttgggcctttatgatttgaaccttgcagctatggtggcattgaattctcagcaggaccctaaagaattccttccttttcttcaagaattggaaagtatgcctgatactttaatgcgctataacattgaccttcggctgcagaggtttgagaaggccctcaagcacattgtttctgcaggagacacttgctatgcagattctatgatcctgatgaagaaaaatcctcaactctttccactgggacttcagctgatagctgatcctaacaagaagaggcaagttctcgacgcttggggagatcatcttagcaatgaaaaatgctttgaggatgctgctaccacttatttgtgctgctccagttttgagaaggctttgaagtcataccgttcttgtggtaattggggtaaggtgcttacagttgccgggcttctaaaactgggtaaagatgagataatgcaactggctcatgagctatgtgaagaattgcaagctcttggtaaaccaaaggaagctgccaaaattgcgctggagtattgtggtgaaattaacaatgggatgagtttgttgattagcgcaagggactgggaggaagctttaagagttgctttaatgcacagtagacaggatttgatctctgaggtgaagaatgcagctttggaatgtgcagtcttgctgattggtgaatacgaggaaggtttggagaaagtagggaagtacttggctcgctacttagctttaaggcaaagaaggttactccttgctgcaaaagtccagtcagaggaacggtcaatgaacgatcttgatgatgatagtgcttcagaagctagcagtaatttcagtggaatgagtgcttacaccactgggactaggaacagctctgccacttccatgagatcaagtgcagctagcagggcaagagatgccaggcgtcagagaaagaaaggaaaaatccgtgctggcagtcctggtgaggagatcgctcttgtagatcacttgaaggggatgcctccaacaagtgaagcactacaggagctaaaatccttgttgcttacgcttatgatgcttggcgaggttgaaactgcaagaaagctgcagaaggctggagagaactttcaacagtcgcatatggcagcagtcaaactagccgaagatacagtatccactgatggcatagatgagcagacacagactttggagcattatacacaaagtactagagatgtagtgcaaaactccgaggctttcttttggcgttgtaaagtatttctttctccttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3H1

Protein Analysis

3054

Amino Acids

340.0

Weight (kDa)

5.48

Isoelectric Point (pI)

45.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3H1 PF10650 948 - 968 2.6e-09 Putative zinc-finger domain
Beta-prop_ELP1_1st PF04762 1735 - 1893 2.9e-27 ELP1 first N-terminal beta-propeller
Beta-prop_ELP1_1st PF04762 1910 - 2063 1.7e-43 ELP1 first N-terminal beta-propeller
Beta-prop_ELP1_2nd PF23797 2097 - 2413 5.3e-48 ELP1 N-terminal second beta-propeller
A-sol_ELP1 PF23925 2438 - 2659 2.5e-47 ELP1 alpha-solenoid
TPR_ELP1 PF23878 2668 - 2829 1.2e-24 ELP1 TPR domain
HB_ELP1 PF23936 2841 - 3001 1.2e-13 ELP1 three-helix bundle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 5070, 7208
AarI CACCTGC 1 cut(s) 7423
Acc36I ACCTGC 5 cut(s) 19, 322, 5392, 7423, 7459
AccB1I GGYRCC 1 cut(s) 7226
AccB7I CCANNNNNTGG 3 cut(s) 1358, 5742, 7286
AccI GTMKAC 5 cut(s) 2160, 2252, 2754, 5553, 8506
AccII CGCG 3 cut(s) 41, 5399, 5748
AccIII TCCGGA 1 cut(s) 5584
AclI AACGTT 3 cut(s) 1521, 2978, 4612
AcoI YGGCCR 6 cut(s) 1345, 2837, 3142, 4163, 6226, 7644
AcuI CTGAAG 8 cut(s) 429, 779, 800, 4908, 5706, 7848, 8117, 8688
AcvI CACGTG 2 cut(s) 341, 3329
AcyI GRCGYC 5 cut(s) 331, 3022, 5550, 7387, 8815
AdeI CACNNNGTG 1 cut(s) 6644
AflII CTTAAG 5 cut(s) 743, 785, 3994, 5804, 5813
AflIII ACRYGT 2 cut(s) 1135, 3420
AgeI ACCGGT 1 cut(s) 3121
AhdI GACNNNNNGTC 2 cut(s) 4812, 6946
AjiI CACGTC 3 cut(s) 1272, 1854, 6997
AjuI GAANNNNNNNTTGG 2 cut(s) 8525, 8557
AleI CACNNNNGTG 2 cut(s) 5162, 6999
AloI GAACNNNNNNTCC 4 cut(s) 3407, 3439, 6034, 6066
Alw21I GWGCWC 5 cut(s) 69, 99, 2614, 3334, 5271
AlwNI CAGNNNCTG 2 cut(s) 1358, 5629
Ama87I CYCGRG 7 cut(s) 349, 462, 654, 4177, 4478, 6373, 6870
Aor13HI TCCGGA 1 cut(s) 5584
ApaI GGGCCC 3 cut(s) 165, 357, 4105
AseI ATTAAT 1 cut(s) 6003
AsiGI ACCGGT 1 cut(s) 3121
Asp700I GAANNNNTTC 5 cut(s) 188, 2130, 2488, 3062, 7956
AsuC2I CCSGG 4 cut(s) 4178, 4179, 6386, 8311
AsuII TTCGAA 2 cut(s) 2118, 4297
AsuNHI GCTAGC 3 cut(s) 4946, 8711, 8792
AvaI CYCGRG 7 cut(s) 349, 462, 654, 4177, 4478, 6373, 6870
BaeGI GKGCMC 5 cut(s) 165, 346, 357, 4105, 5190
BaeI ACNNNNGTAYC 2 cut(s) 1838, 1871
BalI TGGCCA 3 cut(s) 2839, 4165, 7646
BamHI GGATCC 2 cut(s) 1361, 6571
BanI GGYRCC 1 cut(s) 7226
BanII GRGCYC 8 cut(s) 9, 69, 99, 165, 357, 1672, 4105, 5271
BauI CACGAG 3 cut(s) 2685, 5366, 7284
BbrPI CACGTG 2 cut(s) 341, 3329
BbsI GAAGAC 1 cut(s) 6657
Bbv12I GWGCWC 5 cut(s) 69, 99, 2614, 3334, 5271
BceAI ACGGC 6 cut(s) 28, 3157, 4245, 5572, 6053, 6248
BciVI GTATCC 1 cut(s) 9055
BclI TGATCA 6 cut(s) 1060, 2941, 3448, 4453, 7057, 7065
BcnI CCSGG 4 cut(s) 4178, 4179, 6386, 8311
BfoI RGCGCY 2 cut(s) 23, 5202
BfrI CTTAAG 5 cut(s) 743, 785, 3994, 5804, 5813
BfuAI ACCTGC 5 cut(s) 19, 322, 5392, 7423, 7459
BfuI GTATCC 1 cut(s) 9055
BglII AGATCT 2 cut(s) 6055, 6490
BlpI GCTNAGC 1 cut(s) 4471
BmcAI AGTACT 3 cut(s) 3816, 8603, 9100
BmeRI GACNNNNNGTC 2 cut(s) 4812, 6946
BmeT110I CYCGRG 7 cut(s) 349, 462, 654, 4177, 4478, 6373, 6870
BmgBI CACGTC 3 cut(s) 1272, 1854, 6997
BmrI ACTGGG 7 cut(s) 1755, 5622, 5628, 8135, 8333, 8479, 8757
BmtI GCTAGC 3 cut(s) 4950, 8715, 8796
BmuI ACTGGG 7 cut(s) 1755, 5622, 5628, 8135, 8333, 8479, 8757
BoxI GACNNNNGTC 1 cut(s) 2667
BpiI GAAGAC 1 cut(s) 6657
BplI GAGNNNNNCTC 4 cut(s) 2596, 2628, 2668, 2700
BpmI CTGGAG 7 cut(s) 1610, 5228, 5345, 6730, 8227, 8435, 9009
Bpu10I CCTNAGC 2 cut(s) 837, 6724
Bpu1102I GCTNAGC 1 cut(s) 4471
Bpu14I TTCGAA 2 cut(s) 2118, 4297
BpuMI CCSGG 4 cut(s) 4178, 4179, 6386, 8311
BsaAI YACGTR 2 cut(s) 341, 3329
BsaBI GATNNNNATC 1 cut(s) 3957
BsaHI GRCGYC 5 cut(s) 331, 3022, 5550, 7387, 8815
BsaI GGTCTC 3 cut(s) 3130, 5236, 6761
BsaWI WCCGGW 4 cut(s) 3121, 3217, 5584, 7675
BsaXI ACNNNNNCTCC 6 cut(s) 2449, 2479, 3028, 3058, 6930, 6960
Bse118I RCCGGY 1 cut(s) 3121
Bse3DI GCAATG 7 cut(s) 292, 1029, 3710, 4519, 5736, 7345, 8203
Bse8I GATNNNNATC 1 cut(s) 3957
BseAI TCCGGA 1 cut(s) 5584
BseJI GATNNNNATC 1 cut(s) 3957
BseMI GCAATG 7 cut(s) 292, 1029, 3710, 4519, 5736, 7345, 8203
BseRI GAGGAG 4 cut(s) 2457, 5311, 5344, 8873
BseSI GKGCMC 5 cut(s) 165, 346, 357, 4105, 5190
BseX3I CGGCCG 1 cut(s) 3142
BseYI CCCAGC 2 cut(s) 795, 1018
BsgI GTGCAG 7 cut(s) 830, 2753, 5652, 6977, 7719, 8571, 8808
Bsh1236I CGCG 3 cut(s) 41, 5399, 5748
Bsh1285I CGRYCG 1 cut(s) 3145
BshNI GGYRCC 1 cut(s) 7226
BshTI ACCGGT 1 cut(s) 3121
BsiEI CGRYCG 1 cut(s) 3145
BsiHKAI GWGCWC 5 cut(s) 69, 99, 2614, 3334, 5271
BsiHKCI CYCGRG 7 cut(s) 349, 462, 654, 4177, 4478, 6373, 6870
BsiSI CCGG 8 cut(s) 1874, 3122, 3218, 4178, 5585, 6385, 7676, 8310
BsmBI CGTCTC 2 cut(s) 2398, 7526
Bso31I GGTCTC 3 cut(s) 3130, 5236, 6761
BsoBI CYCGRG 7 cut(s) 349, 462, 654, 4177, 4478, 6373, 6870
Bsp119I TTCGAA 2 cut(s) 2118, 4297
Bsp120I GGGCCC 3 cut(s) 161, 353, 4101
Bsp13I TCCGGA 1 cut(s) 5584
Bsp1720I GCTNAGC 1 cut(s) 4471
Bsp19I CCATGG 3 cut(s) 196, 2748, 6601
BspEI TCCGGA 1 cut(s) 5584
BspFNI CGCG 3 cut(s) 41, 5399, 5748
BspHI TCATGA 2 cut(s) 3745, 8353
BspMAI CTGCAG 8 cut(s) 3822, 4374, 5020, 6204, 7028, 8028, 8065, 8982
BspMI ACCTGC 5 cut(s) 19, 322, 5392, 7423, 7459
BspOI GCTAGC 3 cut(s) 4950, 8715, 8796
BspQI GCTCTTC 4 cut(s) 275, 1677, 3318, 5259
BspT104I TTCGAA 2 cut(s) 2118, 4297
BspT107I GGYRCC 1 cut(s) 7226
BspTI CTTAAG 5 cut(s) 743, 785, 3994, 5804, 5813
BspTNI GGTCTC 3 cut(s) 3130, 5236, 6761
BsrDI GCAATG 7 cut(s) 292, 1029, 3710, 4519, 5736, 7345, 8203
BsrFI RCCGGY 1 cut(s) 3121
BssAI RCCGGY 1 cut(s) 3121
BssNI GRCGYC 5 cut(s) 331, 3022, 5550, 7387, 8815
BssSI CACGAG 3 cut(s) 2685, 5366, 7284
BssT1I CCWWGG 7 cut(s) 196, 2748, 2888, 6601, 6655, 6914, 7602
Bst2BI CACGAG 3 cut(s) 2685, 5366, 7284
BstACI GRCGYC 5 cut(s) 331, 3022, 5550, 7387, 8815
BstAFI CTTAAG 5 cut(s) 743, 785, 3994, 5804, 5813
BstAPI GCANNNNNTGC 4 cut(s) 236, 7023, 7099, 8807
BstBAI YACGTR 2 cut(s) 341, 3329
BstBI TTCGAA 2 cut(s) 2118, 4297
BstDSI CCRYGG 5 cut(s) 39, 196, 604, 2748, 6601
BstEII GGTNACC 3 cut(s) 1409, 2558, 3511
BstENI CCTNNNNNAGG 1 cut(s) 3254
BstFNI CGCG 3 cut(s) 41, 5399, 5748
BstH2I RGCGCY 2 cut(s) 23, 5202
BstMCI CGRYCG 1 cut(s) 3145
BstPAI GACNNNNGTC 1 cut(s) 2667
BstPI GGTNACC 3 cut(s) 1409, 2558, 3511
BstSLI GKGCMC 5 cut(s) 165, 346, 357, 4105, 5190
BstUI CGCG 3 cut(s) 41, 5399, 5748
BstV2I GAAGAC 1 cut(s) 6657
BstX2I RGATCY 8 cut(s) 650, 1120, 1361, 1938, 2475, 6055, 6490, 6571
BstXI CCANNNNNNTGG 1 cut(s) 1214
BstYI RGATCY 8 cut(s) 650, 1120, 1361, 1938, 2475, 6055, 6490, 6571
BstZI CGGCCG 1 cut(s) 3142
BsuI GTATCC 1 cut(s) 9055
BtgI CCRYGG 5 cut(s) 39, 196, 604, 2748, 6601
BtrI CACGTC 3 cut(s) 1272, 1854, 6997
BtsI GCAGTG 4 cut(s) 1999, 2331, 6197, 7821
BveI ACCTGC 5 cut(s) 19, 322, 5392, 7423, 7459
CaiI CAGNNNCTG 2 cut(s) 1358, 5629
CciI TCATGA 2 cut(s) 3745, 8353
Cfr10I RCCGGY 1 cut(s) 3121
Cfr42I CCGCGG 1 cut(s) 42
Cfr9I CCCGGG 1 cut(s) 4177
CsiI ACCWGGT 1 cut(s) 859
CspAI ACCGGT 1 cut(s) 3121
DraIII CACNNNGTG 1 cut(s) 6644
DriI GACNNNNNGTC 2 cut(s) 4812, 6946
EaeI YGGCCR 6 cut(s) 1345, 2837, 3142, 4163, 6226, 7644
EagI CGGCCG 1 cut(s) 3142
Eam1105I GACNNNNNGTC 2 cut(s) 4812, 6946
EciI GGCGGA 3 cut(s) 59, 4980, 5314
Ecl136II GAGCTC 3 cut(s) 67, 97, 5269
EclXI CGGCCG 1 cut(s) 3142
Eco130I CCWWGG 7 cut(s) 196, 2748, 2888, 6601, 6655, 6914, 7602
Eco147I AGGCCT 1 cut(s) 5499
Eco24I GRGCYC 8 cut(s) 9, 69, 99, 165, 357, 1672, 4105, 5271
Eco31I GGTCTC 3 cut(s) 3130, 5236, 6761
Eco32I GATATC 1 cut(s) 4418
Eco52I CGGCCG 1 cut(s) 3142
Eco53kI GAGCTC 3 cut(s) 67, 97, 5269
Eco57I CTGAAG 8 cut(s) 429, 779, 800, 4908, 5706, 7848, 8117, 8688
Eco72I CACGTG 2 cut(s) 341, 3329
Eco88I CYCGRG 7 cut(s) 349, 462, 654, 4177, 4478, 6373, 6870
Eco91I GGTNACC 3 cut(s) 1409, 2558, 3511
EcoICRI GAGCTC 3 cut(s) 67, 97, 5269
EcoNI CCTNNNNNAGG 1 cut(s) 3254
EcoO109I RGGNCCY 7 cut(s) 161, 353, 841, 3154, 4101, 7942, 8041
EcoO65I GGTNACC 3 cut(s) 1409, 2558, 3511
EcoRI GAATTC 6 cut(s) 2512, 4182, 6256, 6409, 7930, 7952
EcoRV GATATC 1 cut(s) 4418
EcoT14I CCWWGG 7 cut(s) 196, 2748, 2888, 6601, 6655, 6914, 7602
EcoT22I ATGCAT 5 cut(s) 3629, 4193, 4540, 7304, 7345
EcoT38I GRGCYC 8 cut(s) 9, 69, 99, 165, 357, 1672, 4105, 5271
ErhI CCWWGG 7 cut(s) 196, 2748, 2888, 6601, 6655, 6914, 7602
Esp3I CGTCTC 2 cut(s) 2398, 7526
FauI CCCGC 2 cut(s) 202, 5404
FauNDI CATATG 4 cut(s) 5702, 6251, 7214, 9012
FbaI TGATCA 6 cut(s) 1060, 2941, 3448, 4453, 7057, 7065
FblI GTMKAC 5 cut(s) 2160, 2252, 2754, 5553, 8506
FriOI GRGCYC 8 cut(s) 9, 69, 99, 165, 357, 1672, 4105, 5271
GsaI CCCAGC 2 cut(s) 799, 1022
GsuI CTGGAG 7 cut(s) 1610, 5228, 5345, 6730, 8227, 8435, 9009
HaeII RGCGCY 2 cut(s) 23, 5202
HapII CCGG 8 cut(s) 1874, 3122, 3218, 4178, 5585, 6385, 7676, 8310
Hin1I GRCGYC 5 cut(s) 331, 3022, 5550, 7387, 8815
HincII GTYRAC 6 cut(s) 1243, 1927, 2672, 3439, 5554, 7417
HindII GTYRAC 6 cut(s) 1243, 1927, 2672, 3439, 5554, 7417
HindIII AAGCTT 8 cut(s) 376, 435, 782, 1647, 2241, 3225, 6884, 8478
HpaI GTTAAC 2 cut(s) 1243, 1927
HpaII CCGG 8 cut(s) 1874, 3122, 3218, 4178, 5585, 6385, 7676, 8310
Hpy99I CGWCG 6 cut(s) 14, 5546, 5555, 5558, 6167, 8177
Hsp92I GRCGYC 5 cut(s) 331, 3022, 5550, 7387, 8815
Kpn2I TCCGGA 1 cut(s) 5584
Ksp22I TGATCA 6 cut(s) 1060, 2941, 3448, 4453, 7057, 7065
KspAI GTTAAC 2 cut(s) 1243, 1927
KspI CCGCGG 1 cut(s) 42
LguI GCTCTTC 4 cut(s) 275, 1677, 3318, 5259
MabI ACCWGGT 1 cut(s) 859
MfeI CAATTG 1 cut(s) 1040
MflI RGATCY 8 cut(s) 650, 1120, 1361, 1938, 2475, 6055, 6490, 6571
MlsI TGGCCA 3 cut(s) 2839, 4165, 7646
MluNI TGGCCA 3 cut(s) 2839, 4165, 7646
Mox20I TGGCCA 3 cut(s) 2839, 4165, 7646
Mph1103I ATGCAT 5 cut(s) 3629, 4193, 4540, 7304, 7345
MroI TCCGGA 1 cut(s) 5584
MroXI GAANNNNTTC 5 cut(s) 188, 2130, 2488, 3062, 7956
MscI TGGCCA 3 cut(s) 2839, 4165, 7646
Msp20I TGGCCA 3 cut(s) 2839, 4165, 7646
MspA1I CMGCKG 7 cut(s) 41, 939, 4702, 5918, 7265, 7451, 8137
MspCI CTTAAG 5 cut(s) 743, 785, 3994, 5804, 5813
MspI CCGG 8 cut(s) 1874, 3122, 3218, 4178, 5585, 6385, 7676, 8310
MteI GCGCNGCGC 1 cut(s) 18
MunI CAATTG 1 cut(s) 1040
MvnI CGCG 3 cut(s) 41, 5399, 5748
NciI CCSGG 4 cut(s) 4178, 4179, 6386, 8311
NcoI CCATGG 3 cut(s) 196, 2748, 6601
NdeI CATATG 4 cut(s) 5702, 6251, 7214, 9012
NheI GCTAGC 3 cut(s) 4946, 8711, 8792
NmeAIII GCCGAG 3 cut(s) 934, 5261, 5455
NmuCI GTSAC 8 cut(s) 1409, 2558, 3023, 3500, 4909, 5404, 5767, 7724
NsiI ATGCAT 5 cut(s) 3629, 4193, 4540, 7304, 7345
NspV TTCGAA 2 cut(s) 2118, 4297
OliI CACNNNNGTG 2 cut(s) 5162, 6999
PaeI GCATGC 2 cut(s) 3578, 4538
PaeR7I CTCGAG 4 cut(s) 462, 654, 4478, 6373
PagI TCATGA 2 cut(s) 3745, 8353
PaqCI CACCTGC 1 cut(s) 7423
PceI AGGCCT 1 cut(s) 5499
PciI ACATGT 2 cut(s) 1135, 3420
PciSI GCTCTTC 4 cut(s) 275, 1677, 3318, 5259
PcsI WCGNNNNNNNCGW 4 cut(s) 3147, 5547, 5550, 5559
PdmI GAANNNNTTC 5 cut(s) 188, 2130, 2488, 3062, 7956
PflFI GACNNNGTC 1 cut(s) 6095
PflMI CCANNNNNTGG 3 cut(s) 1358, 5742, 7286
PfoI TCCNGGA 1 cut(s) 6384
PinAI ACCGGT 1 cut(s) 3121
PmaCI CACGTG 2 cut(s) 341, 3329
PmlI CACGTG 2 cut(s) 341, 3329
Ppu21I YACGTR 2 cut(s) 341, 3329
PpuMI RGGWCCY 2 cut(s) 3154, 7942
PscI ACATGT 2 cut(s) 1135, 3420
PshAI GACNNNNGTC 1 cut(s) 2667
PshBI ATTAAT 1 cut(s) 6003
PsiI TTATAA 2 cut(s) 5070, 7208
Psp124BI GAGCTC 3 cut(s) 69, 99, 5271
Psp1406I AACGTT 3 cut(s) 1521, 2978, 4612
Psp5II RGGWCCY 2 cut(s) 3154, 7942
PspCI CACGTG 2 cut(s) 341, 3329
PspEI GGTNACC 3 cut(s) 1409, 2558, 3511
PspFI CCCAGC 2 cut(s) 795, 1018
PspOMI GGGCCC 3 cut(s) 161, 353, 4101
PspPPI RGGWCCY 2 cut(s) 3154, 7942
PspXI VCTCGAGB 1 cut(s) 462
PsrI GAACNNNNNNTAC 4 cut(s) 2674, 2706, 4740, 4772
PstI CTGCAG 8 cut(s) 3822, 4374, 5020, 6204, 7028, 8028, 8065, 8982
PstNI CAGNNNCTG 2 cut(s) 1358, 5629
PsuI RGATCY 8 cut(s) 650, 1120, 1361, 1938, 2475, 6055, 6490, 6571
PsyI GACNNNGTC 1 cut(s) 6095
PvuII CAGCTG 3 cut(s) 4702, 5918, 8137
SacI GAGCTC 3 cut(s) 69, 99, 5271
SacII CCGCGG 1 cut(s) 42
SalI GTCGAC 1 cut(s) 5552
SapI GCTCTTC 4 cut(s) 275, 1677, 3318, 5259
ScaI AGTACT 3 cut(s) 3816, 8603, 9100
SexAI ACCWGGT 1 cut(s) 859
Sfr274I CTCGAG 4 cut(s) 462, 654, 4478, 6373
Sfr303I CCGCGG 1 cut(s) 42
SfuI TTCGAA 2 cut(s) 2118, 4297
SgrBI CCGCGG 1 cut(s) 42
SgrDI CGTCGACG 1 cut(s) 5552
SlaI CTCGAG 4 cut(s) 462, 654, 4478, 6373
SmaI CCCGGG 1 cut(s) 4179
SphI GCATGC 2 cut(s) 3578, 4538
SseBI AGGCCT 1 cut(s) 5499
SspI AATATT 4 cut(s) 2698, 3691, 4511, 5725
SstI GAGCTC 3 cut(s) 69, 99, 5271
StuI AGGCCT 1 cut(s) 5499
StyI CCWWGG 7 cut(s) 196, 2748, 2888, 6601, 6655, 6914, 7602
TaqII GACCGA 1 cut(s) 3519
TatI WGTACW 6 cut(s) 867, 872, 2009, 3814, 8601, 9098
TseFI GTSAC 8 cut(s) 1409, 2558, 3023, 3500, 4909, 5404, 5767, 7724
Tsp45I GTSAC 8 cut(s) 1409, 2558, 3023, 3500, 4909, 5404, 5767, 7724
TspMI CCCGGG 1 cut(s) 4177
Tth111I GACNNNGTC 1 cut(s) 6095
Van91I CCANNNNNTGG 3 cut(s) 1358, 5742, 7286
Vha464I CTTAAG 5 cut(s) 743, 785, 3994, 5804, 5813
VspI ATTAAT 1 cut(s) 6003
XagI CCTNNNNNAGG 1 cut(s) 3254
XbaI TCTAGA 4 cut(s) 617, 6493, 6760, 7294
XcmI CCANNNNNNNNNTGG 4 cut(s) 1355, 1961, 5001, 8410
XhoI CTCGAG 4 cut(s) 462, 654, 4478, 6373
XmaI CCCGGG 1 cut(s) 4177
XmiI GTMKAC 5 cut(s) 2160, 2252, 2754, 5553, 8506
XmnI GAANNNNTTC 5 cut(s) 188, 2130, 2488, 3062, 7956
ZrmI AGTACT 3 cut(s) 3816, 8603, 9100
Zsp2I ATGCAT 5 cut(s) 3629, 4193, 4540, 7304, 7345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.