Rmu_sc0003927.1_g000001

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003927.1
OG0008651
Physical Location & Seq
Forward (+)
831 .. 1214
384 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 384 bp
atgaattacttgtacccagtgaaggaagagtacccgggcgggtcgagttcatcgcagtggagcggggatcctctgatgacggtggctccaccgcagccgatggaggggctgaatgacaccgggcctcctccatttctgaacaagacttatgacatggtggatgacccgagtactaatcggatagtctcgtggagcagaggagggggcagctttgtggtttgggaccctcattcgtttgtgatgaacctcctgcctagatacttcaagcacaataatttctccagctttgtcaggcagcttaacacttacgtaagtgtctttactccccaaagctcaaattggttagtagagatcttcttcttcagaaaagttcagatggtttga

Protein Analysis

127

Amino Acids

14.57

Weight (kDa)

6.04

Isoelectric Point (pI)

45.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 45 - 103 1.4e-20 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 63
AciI CCGC 3 cut(s) 39, 63, 92
AclWI GGATC 2 cut(s) 62, 75
AcuI CTGAAG 1 cut(s) 346
AfaI GTAC 3 cut(s) 14, 32, 172
AfiI CCNNNNNNNGG 2 cut(s) 22, 104
AgsI TTSAA 1 cut(s) 265
AluBI AGCT 4 cut(s) 210, 285, 298, 333
AluI AGCT 4 cut(s) 210, 285, 298, 333
Alw26I GTCTC 1 cut(s) 190
AlwI GGATC 2 cut(s) 62, 75
Ama87I CYCGRG 2 cut(s) 34, 166
AoxI GGCC 1 cut(s) 122
ApeKI GCWGC 3 cut(s) 94, 207, 295
AspS9I GGNCC 2 cut(s) 122, 223
AsuC2I CCSGG 3 cut(s) 35, 36, 121
AvaI CYCGRG 2 cut(s) 34, 166
AvaII GGWCC 1 cut(s) 223
BamHI GGATCC 1 cut(s) 67
BarI GAAGNNNNNNTAC 2 cut(s) 14, 46
BauI CACGAG 1 cut(s) 187
BbvI GCAGC 3 cut(s) 106, 219, 307
BccI CCATC 2 cut(s) 94, 370
BcnI CCSGG 3 cut(s) 35, 36, 121
BcoDI GTCTC 1 cut(s) 190
BfaI CTAG 1 cut(s) 255
BglII AGATCT 1 cut(s) 351
BisI GCNGC 3 cut(s) 95, 208, 296
BlsI GCNGC 3 cut(s) 96, 209, 297
BmcAI AGTACT 1 cut(s) 172
Bme1390I CCNGG 3 cut(s) 35, 36, 121
Bme18I GGWCC 1 cut(s) 223
BmeT110I CYCGRG 2 cut(s) 34, 166
BmgT120I GGNCC 2 cut(s) 122, 223
BmiI GGNNCC 4 cut(s) 69, 87, 224, 225
BmrFI CCNGG 3 cut(s) 35, 36, 121
BmrI ACTGGG 1 cut(s) 11
BmuI ACTGGG 1 cut(s) 11
BpmI CTGGAG 1 cut(s) 265
BpuMI CCSGG 3 cut(s) 35, 36, 121
BsaAI YACGTR 1 cut(s) 310
BsaJI CCNNGG 1 cut(s) 34
BsaXI ACNNNNNCTCC 4 cut(s) 70, 100, 109, 139
Bsc4I CCNNNNNNNGG 2 cut(s) 22, 104
Bse1I ACTGG 1 cut(s) 17
BseDI CCNNGG 1 cut(s) 34
BseGI GGATG 1 cut(s) 166
BseLI CCNNNNNNNGG 2 cut(s) 22, 104
BseNI ACTGG 1 cut(s) 17
BseRI GAGGAG 2 cut(s) 117, 213
BseXI GCAGC 3 cut(s) 106, 219, 307
BshFI GGCC 1 cut(s) 124
BsiHKCI CYCGRG 2 cut(s) 34, 166
BsiSI CCGG 2 cut(s) 35, 120
BslFI GGGAC 1 cut(s) 236
BslI CCNNNNNNNGG 2 cut(s) 22, 104
BsmAI GTCTC 1 cut(s) 190
BsmFI GGGAC 1 cut(s) 236
BsnI GGCC 1 cut(s) 124
BsoBI CYCGRG 2 cut(s) 34, 166
Bsp143I GATC 2 cut(s) 67, 351
BspACI CCGC 3 cut(s) 39, 63, 92
BspANI GGCC 1 cut(s) 124
BspLI GGNNCC 4 cut(s) 69, 87, 224, 225
BspPI GGATC 2 cut(s) 62, 75
BsrBI CCGCTC 1 cut(s) 63
BsrI ACTGG 1 cut(s) 17
BssECI CCNNGG 1 cut(s) 34
BssMI GATC 2 cut(s) 67, 351
BssSI CACGAG 1 cut(s) 187
Bst2BI CACGAG 1 cut(s) 187
Bst4CI ACNGT 1 cut(s) 82
Bst6I CTCTTC 1 cut(s) 21
BstBAI YACGTR 1 cut(s) 310
BstF5I GGATG 1 cut(s) 166
BstKTI GATC 2 cut(s) 70, 354
BstMAI GTCTC 1 cut(s) 190
BstMBI GATC 2 cut(s) 67, 351
BstSCI CCNGG 3 cut(s) 33, 34, 119
BstSNI TACGTA 1 cut(s) 310
BstV1I GCAGC 3 cut(s) 106, 219, 307
BstX2I RGATCY 2 cut(s) 67, 351
BstYI RGATCY 2 cut(s) 67, 351
BsuRI GGCC 1 cut(s) 124
BtgZI GCGATG 1 cut(s) 36
BtsCI GGATG 1 cut(s) 166
BtsI GCAGTG 1 cut(s) 62
BtsIMutI CAGTG 2 cut(s) 24, 62
Cfr13I GGNCC 2 cut(s) 122, 223
Cfr9I CCCGGG 1 cut(s) 34
Csp6I GTAC 3 cut(s) 13, 31, 171
CviAII CATG 1 cut(s) 154
CviJI RGCY 8 cut(s) 86, 97, 109, 124, 210, 285, 298, 333
CviKI_1 RGCY 8 cut(s) 86, 97, 109, 124, 210, 285, 298, 333
CviQI GTAC 3 cut(s) 13, 31, 171
DpnI GATC 2 cut(s) 69, 353
DpnII GATC 2 cut(s) 67, 351
Eam1104I CTCTTC 1 cut(s) 21
EarI CTCTTC 1 cut(s) 21
Eco105I TACGTA 1 cut(s) 310
Eco47I GGWCC 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 346
Eco88I CYCGRG 2 cut(s) 34, 166
EcoO109I RGGNCCY 1 cut(s) 223
FaeI CATG 1 cut(s) 157
FaiI YATR 2 cut(s) 150, 155
FaqI GGGAC 1 cut(s) 236
FatI CATG 1 cut(s) 153
FauI CCCGC 2 cut(s) 32, 56
Fnu4HI GCNGC 3 cut(s) 95, 208, 296
FokI GGATG 1 cut(s) 173
Fsp4HI GCNGC 3 cut(s) 95, 208, 296
FspBI CTAG 1 cut(s) 255
GluI GCNGC 3 cut(s) 95, 208, 296
GsuI CTGGAG 1 cut(s) 265
HaeIII GGCC 1 cut(s) 124
HapII CCGG 2 cut(s) 35, 120
Hin1II CATG 1 cut(s) 157
HpaII CCGG 2 cut(s) 35, 120
Hpy188I TCNGA 5 cut(s) 75, 138, 180, 365, 375
HpyAV CCTTC 1 cut(s) 16
HpyCH4III ACNGT 1 cut(s) 82
HpyCH4IV ACGT 1 cut(s) 309
HpySE526I ACGT 1 cut(s) 309
Hsp92II CATG 1 cut(s) 157
KflI GGGWCCC 1 cut(s) 223
Kzo9I GATC 2 cut(s) 67, 351
LmnI GCTCC 3 cut(s) 60, 91, 192
LpnPI CCDG 6 cut(s) 30, 48, 133, 263, 277, 295
Lsp1109I GCAGC 3 cut(s) 106, 219, 307
MaeI CTAG 1 cut(s) 255
MaeII ACGT 1 cut(s) 309
MalI GATC 2 cut(s) 69, 353
MbiI CCGCTC 1 cut(s) 63
MboI GATC 2 cut(s) 67, 351
MboII GAAGA 4 cut(s) 38, 346, 349, 352
MflI RGATCY 2 cut(s) 67, 351
MluCI AATT 3 cut(s) 4, 274, 337
MnlI CCTC 8 cut(s) 81, 97, 135, 138, 191, 194, 237, 257
MseI TTAA 1 cut(s) 300
MslI CAYNNNNRTG 1 cut(s) 55
MspI CCGG 2 cut(s) 35, 120
MspR9I CCNGG 3 cut(s) 35, 36, 121
NciI CCSGG 3 cut(s) 35, 36, 121
NdeII GATC 2 cut(s) 67, 351
NlaIII CATG 1 cut(s) 157
NlaIV GGNNCC 4 cut(s) 69, 87, 224, 225
PkrI GCNGC 3 cut(s) 96, 209, 297
Ppu21I YACGTR 1 cut(s) 310
PpuMI RGGWCCY 1 cut(s) 223
Psp5II RGGWCCY 1 cut(s) 223
PspN4I GGNNCC 4 cut(s) 69, 87, 224, 225
PspPI GGNCC 2 cut(s) 122, 223
PspPPI RGGWCCY 1 cut(s) 223
PsuI RGATCY 2 cut(s) 67, 351
RsaI GTAC 3 cut(s) 14, 32, 172
RsaNI GTAC 3 cut(s) 13, 31, 171
RseI CAYNNNNRTG 1 cut(s) 55
SaqAI TTAA 1 cut(s) 300
SatI GCNGC 3 cut(s) 95, 208, 296
Sau3AI GATC 2 cut(s) 67, 351
Sau96I GGNCC 2 cut(s) 122, 223
ScaI AGTACT 1 cut(s) 172
ScrFI CCNGG 3 cut(s) 35, 36, 121
SetI ASST 6 cut(s) 212, 249, 287, 300, 312, 335
SinI GGWCC 1 cut(s) 223
SmaI CCCGGG 1 cut(s) 36
SmiMI CAYNNNNRTG 1 cut(s) 55
SnaBI TACGTA 1 cut(s) 310
Sse9I AATT 3 cut(s) 4, 274, 337
SsiI CCGC 3 cut(s) 39, 63, 92
SspMI CTAG 1 cut(s) 255
StyD4I CCNGG 3 cut(s) 33, 34, 119
TaaI ACNGT 1 cut(s) 82
TaiI ACGT 1 cut(s) 312
TaqI TCGA 1 cut(s) 44
TasI AATT 3 cut(s) 4, 274, 337
TatI WGTACW 1 cut(s) 170
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TscAI CASTG 2 cut(s) 24, 62
TseI GCWGC 3 cut(s) 94, 207, 295
TspDTI ATGAA 3 cut(s) 17, 39, 257
TspMI CCCGGG 1 cut(s) 34
TspRI CASTG 2 cut(s) 24, 62
VpaK11BI GGWCC 1 cut(s) 223
XmaI CCCGGG 1 cut(s) 34
XspI CTAG 1 cut(s) 255
ZrmI AGTACT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.