Rmu_sc0003929.1_g000003

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003929.1
OG0013749
Physical Location & Seq
Reverse (-)
9175 .. 13969
4795 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 918 bp
atgggcttcaaaagccctcaagaatcaccaggaaaggtggccggaaaaagaagaaccgatggaggcgatttcttccccgatgaggttctcttctcggttattggattatccgtaacaggggcccttgatgcaatcttgccacacgagcattcaaaggagatccgccgctatctttataaccatcagaacaaagatggtggttggggactgcatatagaagggccaagtacaatgttttgtacagctttgtcctatgtgagtttgagattgctcggagaggagatggatggcagtgatggcgccatggagaaggcaagaaaatggattcttcaccgtggaggagttaccaccatcccatcatggggaaagatgtggctctcagtttccctattgcgcctcacttttttctttactcccctcgtccaactgcgtctcagtgctggctctggtctttggcagcaccagcgcttgatggatgtggtcggaccggatagctgtttcgtgagtgtgttgactgtattcgtatctcgaaaactggggttggagagggttttaatggtgttgggtccttgccggatatttgtgatgtctcacgaaagcagagtggaggctgattttcgccggttcagagtgtggatgcatggctgtgggctgggagcaataatacattttcagatcggtggatcgtgctggccggattgtcagattggactttcgatctggtttctggccacaccggcttggggttgggatgggatttgggatctttcgctggtgtcccgttggcgacagggtggtaagggttcgacgtcggtactcgtgcaacggcatggcgtggtcgcaggtgatggcgatttggagcatttgtggattgccagtgttgacattaaggtggcaatggagttgggtttagtttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000250 GO:0003674 GO:0003824 GO:0016853 GO:0016866 GO:0031559
KEGG Pathways
Metabolic & Signaling
ko00100 ko01100 ko01110 ko01130 map00100 map01100 map01110 map01130
Pfam Domains
Protein Families
SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

305

Amino Acids

33.89

Weight (kDa)

8.23

Isoelectric Point (pI)

43.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_N PF13249 48 - 128 7.7e-19 Squalene-hopene cyclase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 177
AarI CACCTGC 1 cut(s) 833
AatII GACGTC 1 cut(s) 812
Acc36I ACCTGC 1 cut(s) 833
AccB1I GGYRCC 1 cut(s) 299
AciI CCGC 2 cut(s) 163, 166
AclWI GGATC 3 cut(s) 154, 691, 771
AcoI YGGCCR 3 cut(s) 39, 692, 729
AcyI GRCGYC 2 cut(s) 300, 809
AfaI GTAC 3 cut(s) 229, 241, 816
AfeI AGCGCT 1 cut(s) 467
AfiI CCNNNNNNNGG 5 cut(s) 82, 117, 361, 362, 743
AgsI TTSAA 2 cut(s) 10, 153
AjnI CCWGG 1 cut(s) 28
AluBI AGCT 2 cut(s) 245, 495
AluI AGCT 2 cut(s) 245, 495
Alw26I GTCTC 2 cut(s) 437, 594
AlwI GGATC 3 cut(s) 154, 691, 771
Aor51HI AGCGCT 1 cut(s) 467
AoxI GGCC 5 cut(s) 39, 120, 221, 692, 729
ApaI GGGCCC 1 cut(s) 124
ApeKI GCWGC 1 cut(s) 457
AspLEI GCGC 3 cut(s) 302, 396, 468
AspS9I GGNCC 5 cut(s) 120, 121, 221, 485, 566
AsuHPI GGTGA 3 cut(s) 18, 323, 857
AvaII GGWCC 2 cut(s) 485, 566
BaeGI GKGCMC 1 cut(s) 124
BalI TGGCCA 1 cut(s) 731
BanI GGYRCC 1 cut(s) 299
BanII GRGCYC 1 cut(s) 124
BauI CACGAG 2 cut(s) 143, 818
BbvI GCAGC 1 cut(s) 469
BceAI ACGGC 1 cut(s) 842
BciT130I CCWGG 1 cut(s) 30
BcoDI GTCTC 2 cut(s) 437, 594
BfoI RGCGCY 2 cut(s) 303, 469
BfuAI ACCTGC 1 cut(s) 833
BglI GCCNNNNNGGC 1 cut(s) 737
BisI GCNGC 2 cut(s) 166, 458
BlsI GCNGC 2 cut(s) 167, 459
Bme1390I CCNGG 1 cut(s) 30
Bme18I GGWCC 2 cut(s) 485, 566
BmgT120I GGNCC 5 cut(s) 120, 121, 221, 485, 566
BmiI GGNNCC 4 cut(s) 121, 122, 301, 567
BmrFI CCNGG 1 cut(s) 30
BmrI ACTGGG 1 cut(s) 545
BmsI GCATC 2 cut(s) 118, 627
BmuI ACTGGG 1 cut(s) 545
BsaHI GRCGYC 2 cut(s) 300, 809
BsaJI CCNNGG 2 cut(s) 303, 334
BsaWI WCCGGW 1 cut(s) 487
BsaXI ACNNNNNCTCC 2 cut(s) 851, 881
Bsc4I CCNNNNNNNGG 5 cut(s) 82, 117, 361, 362, 743
Bse118I RCCGGY 2 cut(s) 621, 736
Bse1I ACTGG 2 cut(s) 540, 876
Bse3DI GCAATG 1 cut(s) 903
BseBI CCWGG 1 cut(s) 30
BseDI CCNNGG 2 cut(s) 303, 334
BseGI GGATG 5 cut(s) 292, 351, 481, 642, 757
BseLI CCNNNNNNNGG 5 cut(s) 82, 117, 361, 362, 743
BseMI GCAATG 1 cut(s) 903
BseMII CTCAG 2 cut(s) 393, 448
BseNI ACTGG 2 cut(s) 540, 876
BseRI GAGGAG 2 cut(s) 293, 354
BseSI GKGCMC 1 cut(s) 124
BseXI GCAGC 1 cut(s) 469
BseYI CCCAGC 1 cut(s) 652
BshFI GGCC 5 cut(s) 41, 122, 223, 694, 731
BshNI GGYRCC 1 cut(s) 299
BsiSI CCGG 6 cut(s) 42, 488, 574, 622, 695, 737
BslFI GGGAC 2 cut(s) 219, 763
BslI CCNNNNNNNGG 5 cut(s) 82, 117, 361, 362, 743
BsmAI GTCTC 2 cut(s) 437, 594
BsmBI CGTCTC 1 cut(s) 437
BsmFI GGGAC 2 cut(s) 219, 763
BsmI GAATGC 1 cut(s) 148
BsnI GGCC 5 cut(s) 41, 122, 223, 694, 731
Bsp120I GGGCCC 1 cut(s) 120
Bsp1286I GDGCHC 1 cut(s) 124
Bsp1407I TGTACA 1 cut(s) 239
Bsp143I GATC 5 cut(s) 159, 675, 683, 717, 763
Bsp19I CCATGG 1 cut(s) 303
BspACI CCGC 2 cut(s) 163, 166
BspANI GGCC 5 cut(s) 41, 122, 223, 694, 731
BspCNI CTCAG 2 cut(s) 392, 447
BspLI GGNNCC 4 cut(s) 121, 122, 301, 567
BspMI ACCTGC 1 cut(s) 833
BspPI GGATC 3 cut(s) 154, 691, 771
BspT107I GGYRCC 1 cut(s) 299
BsrDI GCAATG 1 cut(s) 903
BsrFI RCCGGY 2 cut(s) 621, 736
BsrGI TGTACA 1 cut(s) 239
BsrI ACTGG 2 cut(s) 540, 876
BssAI RCCGGY 2 cut(s) 621, 736
BssECI CCNNGG 2 cut(s) 303, 334
BssMI GATC 5 cut(s) 159, 675, 683, 717, 763
BssNI GRCGYC 2 cut(s) 300, 809
BssSI CACGAG 2 cut(s) 143, 818
BssT1I CCWWGG 1 cut(s) 303
Bst2BI CACGAG 2 cut(s) 143, 818
Bst2UI CCWGG 1 cut(s) 30
Bst4CI ACNGT 2 cut(s) 335, 517
Bst6I CTCTTC 1 cut(s) 95
BstACI GRCGYC 2 cut(s) 300, 809
BstAUI TGTACA 1 cut(s) 239
BstC8I GCNNGC 2 cut(s) 442, 692
BstDEI CTNAG 2 cut(s) 379, 434
BstDSI CCRYGG 2 cut(s) 303, 334
BstF5I GGATG 5 cut(s) 292, 351, 481, 642, 757
BstH2I RGCGCY 2 cut(s) 303, 469
BstHHI GCGC 3 cut(s) 302, 396, 468
BstKTI GATC 5 cut(s) 162, 678, 686, 720, 766
BstMAI GTCTC 2 cut(s) 437, 594
BstMBI GATC 5 cut(s) 159, 675, 683, 717, 763
BstMWI GCNNNNNNNGC 6 cut(s) 12, 128, 145, 297, 463, 737
BstNI CCWGG 1 cut(s) 30
BstSCI CCNGG 1 cut(s) 28
BstSLI GKGCMC 1 cut(s) 124
BstV1I GCAGC 1 cut(s) 469
BstX2I RGATCY 2 cut(s) 159, 763
BstYI RGATCY 2 cut(s) 159, 763
BsuRI GGCC 5 cut(s) 41, 122, 223, 694, 731
BtgI CCRYGG 2 cut(s) 303, 334
BtsCI GGATG 5 cut(s) 292, 351, 481, 642, 757
BtsI GCAGTG 1 cut(s) 298
BtsIMutI CAGTG 3 cut(s) 298, 442, 883
BveI ACCTGC 1 cut(s) 833
Cac8I GCNNGC 2 cut(s) 442, 692
CfoI GCGC 3 cut(s) 302, 396, 468
Cfr10I RCCGGY 2 cut(s) 621, 736
Cfr13I GGNCC 5 cut(s) 120, 121, 221, 485, 566
CpoI CGGWCCG 1 cut(s) 485
CseI GACGC 1 cut(s) 419
Csp6I GTAC 3 cut(s) 228, 240, 815
CspCI CAANNNNNGTGG 2 cut(s) 178, 213
CspI CGGWCCG 1 cut(s) 485
CviAII CATG 4 cut(s) 304, 360, 641, 830
CviQI GTAC 3 cut(s) 228, 240, 815
DdeI CTNAG 2 cut(s) 379, 434
DinI GGCGCC 1 cut(s) 301
DpnI GATC 5 cut(s) 161, 677, 685, 719, 765
DpnII GATC 5 cut(s) 159, 675, 683, 717, 763
EaeI YGGCCR 3 cut(s) 39, 692, 729
Eam1104I CTCTTC 1 cut(s) 95
EarI CTCTTC 1 cut(s) 95
EciI GGCGGA 1 cut(s) 152
Eco130I CCWWGG 1 cut(s) 303
Eco24I GRGCYC 1 cut(s) 124
Eco47I GGWCC 2 cut(s) 485, 566
Eco47III AGCGCT 1 cut(s) 467
EcoO109I RGGNCCY 3 cut(s) 120, 121, 566
EcoRII CCWGG 1 cut(s) 28
EcoT14I CCWWGG 1 cut(s) 303
EcoT22I ATGCAT 1 cut(s) 642
EcoT38I GRGCYC 1 cut(s) 124
EgeI GGCGCC 1 cut(s) 301
EheI GGCGCC 1 cut(s) 301
ErhI CCWWGG 1 cut(s) 303
Esp3I CGTCTC 1 cut(s) 437
FaeI CATG 4 cut(s) 307, 363, 644, 833
FaiI YATR 8 cut(s) 177, 213, 215, 255, 305, 361, 642, 831
FaqI GGGAC 2 cut(s) 219, 763
FatI CATG 4 cut(s) 303, 359, 640, 829
Fnu4HI GCNGC 2 cut(s) 166, 458
FokI GGATG 5 cut(s) 299, 338, 488, 649, 764
FriOI GRGCYC 1 cut(s) 124
Fsp4HI GCNGC 2 cut(s) 166, 458
GlaI GCGC 3 cut(s) 301, 395, 467
GluI GCNGC 2 cut(s) 166, 458
GsaI CCCAGC 1 cut(s) 656
HaeII RGCGCY 2 cut(s) 303, 469
HaeIII GGCC 5 cut(s) 41, 122, 223, 694, 731
HapII CCGG 6 cut(s) 42, 488, 574, 622, 695, 737
HgaI GACGC 1 cut(s) 419
HhaI GCGC 3 cut(s) 302, 396, 468
Hin1I GRCGYC 2 cut(s) 300, 809
Hin1II CATG 4 cut(s) 307, 363, 644, 833
Hin6I GCGC 3 cut(s) 300, 394, 466
HinP1I GCGC 3 cut(s) 300, 394, 466
HincII GTYRAC 2 cut(s) 513, 883
HindII GTYRAC 2 cut(s) 513, 883
HinfI GANTC 2 cut(s) 23, 325
HpaII CCGG 6 cut(s) 42, 488, 574, 622, 695, 737
HphI GGTGA 3 cut(s) 18, 323, 857
Hpy166II GTNNAC 2 cut(s) 513, 883
Hpy188I TCNGA 6 cut(s) 186, 275, 485, 629, 675, 705
Hpy188III TCNNGA 4 cut(s) 20, 502, 528, 593
Hpy8I GTNNAC 2 cut(s) 513, 883
Hpy99I CGWCG 2 cut(s) 811, 814
HpyAV CCTTC 2 cut(s) 212, 304
HpyCH4III ACNGT 2 cut(s) 335, 517
HpyCH4IV ACGT 1 cut(s) 809
HpyCH4V TGCA 4 cut(s) 131, 211, 640, 823
HpyF10VI GCNNNNNNNGC 6 cut(s) 12, 128, 145, 297, 463, 737
HpyF3I CTNAG 2 cut(s) 379, 434
HpySE526I ACGT 1 cut(s) 809
Hsp92I GRCGYC 2 cut(s) 300, 809
Hsp92II CATG 4 cut(s) 307, 363, 644, 833
HspAI GCGC 3 cut(s) 300, 394, 466
KasI GGCGCC 1 cut(s) 299
Kzo9I GATC 5 cut(s) 159, 675, 683, 717, 763
LmnI GCTCC 2 cut(s) 656, 859
Lsp1109I GCAGC 1 cut(s) 469
LweI GCATC 2 cut(s) 118, 627
MaeII ACGT 1 cut(s) 809
MaeIII GTNAC 2 cut(s) 112, 343
MalI GATC 5 cut(s) 161, 677, 685, 719, 765
MboI GATC 5 cut(s) 159, 675, 683, 717, 763
MboII GAAGA 4 cut(s) 63, 64, 82, 320
MflI RGATCY 2 cut(s) 159, 763
MhlI GDGCHC 1 cut(s) 124
MlsI TGGCCA 1 cut(s) 731
MluNI TGGCCA 1 cut(s) 731
Mly113I GGCGCC 1 cut(s) 300
MmeI TCCRAC 3 cut(s) 448, 463, 522
MnlI CCTC 9 cut(s) 27, 56, 76, 271, 332, 407, 428, 540, 601
Mox20I TGGCCA 1 cut(s) 731
Mph1103I ATGCAT 1 cut(s) 642
MscI TGGCCA 1 cut(s) 731
MseI TTAA 2 cut(s) 554, 888
MslI CAYNNNNRTG 2 cut(s) 645, 890
Msp20I TGGCCA 1 cut(s) 731
MspI CCGG 6 cut(s) 42, 488, 574, 622, 695, 737
MspR9I CCNGG 1 cut(s) 30
Mva1269I GAATGC 1 cut(s) 148
MvaI CCWGG 1 cut(s) 30
MwoI GCNNNNNNNGC 6 cut(s) 12, 128, 145, 297, 463, 737
NarI GGCGCC 1 cut(s) 300
NcoI CCATGG 1 cut(s) 303
NdeII GATC 5 cut(s) 159, 675, 683, 717, 763
NlaIII CATG 4 cut(s) 307, 363, 644, 833
NlaIV GGNNCC 4 cut(s) 121, 122, 301, 567
NsiI ATGCAT 1 cut(s) 642
PaqCI CACCTGC 1 cut(s) 833
PctI GAATGC 1 cut(s) 148
PfeI GAWTC 2 cut(s) 23, 325
PkrI GCNGC 2 cut(s) 167, 459
PluTI GGCGCC 1 cut(s) 303
PpuMI RGGWCCY 1 cut(s) 566
PsiI TTATAA 1 cut(s) 177
Psp5II RGGWCCY 1 cut(s) 566
Psp6I CCWGG 1 cut(s) 28
PspFI CCCAGC 1 cut(s) 652
PspGI CCWGG 1 cut(s) 28
PspN4I GGNNCC 4 cut(s) 121, 122, 301, 567
PspOMI GGGCCC 1 cut(s) 120
PspPI GGNCC 5 cut(s) 120, 121, 221, 485, 566
PspPPI RGGWCCY 1 cut(s) 566
PsuI RGATCY 2 cut(s) 159, 763
RsaI GTAC 3 cut(s) 229, 241, 816
RsaNI GTAC 3 cut(s) 228, 240, 815
RseI CAYNNNNRTG 2 cut(s) 645, 890
Rsr2I CGGWCCG 1 cut(s) 485
RsrII CGGWCCG 1 cut(s) 485
SaqAI TTAA 2 cut(s) 554, 888
SatI GCNGC 2 cut(s) 166, 458
Sau3AI GATC 5 cut(s) 159, 675, 683, 717, 763
Sau96I GGNCC 5 cut(s) 120, 121, 221, 485, 566
ScrFI CCNGG 1 cut(s) 30
SduI GDGCHC 1 cut(s) 124
SetI ASST 7 cut(s) 39, 87, 247, 497, 812, 847, 894
SfaNI GCATC 2 cut(s) 118, 627
SfoI GGCGCC 1 cut(s) 301
SinI GGWCC 2 cut(s) 485, 566
SmiMI CAYNNNNRTG 2 cut(s) 645, 890
SmlI CTYRAG 1 cut(s) 18
SmoI CTYRAG 1 cut(s) 18
SsiI CCGC 2 cut(s) 163, 166
SspDI GGCGCC 1 cut(s) 299
StyD4I CCNGG 1 cut(s) 28
StyI CCWWGG 1 cut(s) 303
TaaI ACNGT 2 cut(s) 335, 517
TaiI ACGT 1 cut(s) 812
TaqI TCGA 3 cut(s) 529, 716, 806
TatI WGTACW 2 cut(s) 227, 239
TauI GCSGC 1 cut(s) 168
TfiI GAWTC 2 cut(s) 23, 325
Tru1I TTAA 2 cut(s) 554, 888
Tru9I TTAA 2 cut(s) 554, 888
TscAI CASTG 3 cut(s) 298, 442, 883
TseI GCWGC 1 cut(s) 457
TspGWI ACGGA 1 cut(s) 100
TspRI CASTG 3 cut(s) 298, 442, 883
VpaK11BI GGWCC 2 cut(s) 485, 566
ZraI GACGTC 1 cut(s) 810
Zsp2I ATGCAT 1 cut(s) 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.