Rmu_sc0003936.1_g000037

F-box WD-40 repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003936.1
OG0004801
Physical Location & Seq
Forward (+)
165104 .. 171959
6856 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2367 bp
atgtctcttacattcttctccaggcaggagctggccaaggccatcaacaacttctctcccaactccattatctgtgtaggtggattcggtccgatatacaaggctgagctctccaatggctctactgtggctttgaaatatttggactcaaatgcatttggagggttctcgtatattcatgctgaaataaaaacattagggaagctccgccatcccaatcttgtcaagcttctaggacgttgcatgtccagttccggcaggtttcttgtttatgagttcatcgagaatggtagtctccacgagtggctgcatggacagcggggagtccgggatccgatggcattgccttgggaaacaagaatgaagattgtgaagggtgttgctgatgctcttaaatatttgcatggattgcaaagaccagtcattcacagggatataaaggccggccatgtgttattggactctaattttgaggctcatcttactgattttgggctggctcgaatgttggaaccctcagagacgcacttgtcaacctatatcaatgctggttctttctggtataggccaccggagtatttattcagtaaggcatcatgggcgacattgaaaggcgatgtatacagctttggagttttgatgttggagatagcatctgggaagcctccgagttccatggacatccatccttccttcaggttacggcttcctcaatgtgccaagaagcttgttgcccaaaacatgcacatggatttagtggattcaaggattttgaggcaggacttaaacgaagctagtgttatagagtattttaggattgctcttagttgtctcaaaaaagaccccaagaaaagacctgaaatgagtaaggttgtacaaatgttgaatgaacttcccattattgagcatgatcttgacctcatcatctttgaagaccctcatgtccatgcttctcaagctttccttgatgatattgatgggctggatagcttcccggcggagatggatcccagtcgcgtcggcagcaagaagaggccaaccagaagctcaagagctccggccagaatccaatctctcgaccacgacatcctctgtatcatcttctcttttctcgacttcttcgacctcgcacgttgttccgtcgtctgcaaaacctggagtggtattgtcaacaagtcaaagttgctagaagcgctttactacaagaagtggcagatgggtcttgtgggttctgagaataggtccagtgggtgcccagagaaatctttgaatgtttatttggaggagatggctctgcagcatcatagactggcattacagctaggtcgaaattggagcacaacaactgctacagatatcgaaggcacatatgtggatgatatcattttcctaaccttgctttctattagggttgatcagtgccgaaagaagatgggtttgcttcttactggcattggtgataaggttatgcgcctttggtcattggaaagctataaatgtgtgggagaatatcccattccagacaaactccctttagttgactttgattttgatgagagcaagattgttggtttggtcggcagtcgtatatgcatatggaggcggaatgggataagaagtatattcccctcgcgtgagggcacctttccaaagggcttatgcatgcgttactgcgatcctgatgctgtggttggctgtgtggatgggacagctcgtgtatttgacatgtacagtcaacaatgttctcaaatcgttaaaatgcatgctggtccagtgacatgcttgtgtttgactgatgatcagttgatacttagcggttcttctcttggcagtatcacggtgtctaatcttttatctgatcagcgggttgcagaattaagactgacacattccacaggcataaggactttgtgtttcaacccctcatctcaccagctgtttgctggatcaactgctggatatgtattttgttgggacctcaggacaaggagatgtttatgggagacacgagtgagtccaaatgaggtatattcaatgcaacacctgagaaatgatgcatcaagcttggtggttggtggaatagatggtgtgctacgtatactgaatcagaacacaggtgaagtagtttcaagatttgttttggaaggcaacttattgtcaagttcaagttcaagttcaagttctcatcaacacgtagtgggaagtggcactgtccaaagaatgacaggacgaaggctttcacaaaatgcctcaattgatagcattcctagaagcagtcgacctcccatcacttgcttagcagtcgggatgaagaaggttatcaccacacacaataataagtacatcagaacgtggaagttcaactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
F-box F-box-like WD40

Protein Analysis

788

Amino Acids

88.34

Weight (kDa)

8.86

Isoelectric Point (pI)

42.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 22 - 293 8.8e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 24 - 295 5.6e-39 Protein tyrosine and serine/threonine kinase
F-box PF00646 356 - 395 2.3e-09 F-box domain
F-box-like PF12937 361 - 395 2.8e-08 F-box-like
Beta-prop_EML PF23409 487 - 653 2.6e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_ACSF4 PF13570 553 - 736 1.2e-13 Beta-alanine-activating enzyme, beta-propeller
WD40_WDHD1_1st PF24817 553 - 767 1.4e-10 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 562 - 700 6.9e-17 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 562 - 721 7e-13 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 562 - 712 4.3e-10 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 563 - 684 9e-08 WDR36/Utp21 first beta-propeller
Beta-prop_WDR5 PF25175 566 - 729 1.3e-14 WDR5 beta-propeller domain
WDR55 PF24796 567 - 709 2e-10 WDR55
Beta-prop_THOC3 PF25174 599 - 717 1.8e-08 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 636 - 719 9.7e-07 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 529, 941
Acc36I ACCTGC 1 cut(s) 249
AccB1I GGYRCC 2 cut(s) 1251, 1640
AccI GTMKAC 3 cut(s) 621, 2098, 2278
AccII CGCG 2 cut(s) 1017, 1633
AciI CCGC 6 cut(s) 208, 319, 998, 1603, 1815, 1864
AclWI GGATC 6 cut(s) 326, 339, 1001, 1014, 1670, 1954
AcoI YGGCCR 3 cut(s) 33, 445, 1059
AcuI CTGAAG 1 cut(s) 679
AdeI CACNNNGTG 1 cut(s) 2197
AfaI GTAC 3 cut(s) 876, 1730, 2342
AfeI AGCGCT 1 cut(s) 1194
AflIII ACRYGT 2 cut(s) 1725, 2191
AjnI CCWGG 2 cut(s) 20, 1154
AjuI GAANNNNNNNTTGG 2 cut(s) 1262, 1294
AleI CACNNNNGTG 1 cut(s) 1370
Alw21I GWGCWC 3 cut(s) 111, 1057, 1340
Alw26I GTCTC 5 cut(s) 9, 299, 515, 836, 1997
AlwI GGATC 6 cut(s) 326, 339, 1001, 1014, 1670, 1954
AlwNI CAGNNNCTG 1 cut(s) 31
Aor51HI AGCGCT 1 cut(s) 1194
AoxI GGCC 7 cut(s) 33, 39, 441, 445, 566, 1034, 1059
ApeKI GCWGC 3 cut(s) 307, 1023, 1297
ArsI GACNNNNNNTTYG 4 cut(s) 452, 484, 1532, 1564
Asp700I GAANNNNTTC 1 cut(s) 2236
AspLEI GCGC 2 cut(s) 1195, 1473
AspS9I GGNCC 4 cut(s) 89, 1242, 1769, 1975
AsuC2I CCSGG 2 cut(s) 329, 995
AsuHPI GGTGA 4 cut(s) 1469, 1922, 2129, 2314
AvaII GGWCC 4 cut(s) 89, 1242, 1769, 1975
AxyI CCTNAGG 1 cut(s) 1979
BaeGI GKGCMC 2 cut(s) 1256, 1643
BalI TGGCCA 1 cut(s) 35
BamHI GGATCC 2 cut(s) 331, 1006
BanI GGYRCC 2 cut(s) 1251, 1640
BanII GRGCYC 2 cut(s) 111, 1057
BauI CACGAG 3 cut(s) 299, 1713, 2007
BbsI GAAGAC 1 cut(s) 939
Bbv12I GWGCWC 3 cut(s) 111, 1057, 1340
BbvI GCAGC 3 cut(s) 294, 1035, 1309
BceAI ACGGC 1 cut(s) 719
BcgI CGANNNNNNTGC 4 cut(s) 325, 359, 1664, 1698
BciT130I CCWGG 2 cut(s) 22, 1156
BclI TGATCA 3 cut(s) 1414, 1798, 1858
BcnI CCSGG 2 cut(s) 329, 995
BcoDI GTCTC 5 cut(s) 9, 299, 515, 836, 1997
BfaI CTAG 5 cut(s) 233, 795, 1187, 1322, 2268
BfmI CTRYAG 2 cut(s) 1295, 1350
BfoI RGCGCY 1 cut(s) 1196
BfuAI ACCTGC 1 cut(s) 249
BisI GCNGC 3 cut(s) 308, 1024, 1298
BlpI GCTNAGC 2 cut(s) 105, 2296
BlsI GCNGC 3 cut(s) 309, 1025, 1299
Bme1390I CCNGG 4 cut(s) 22, 329, 995, 1156
Bme18I GGWCC 4 cut(s) 89, 1242, 1769, 1975
BmgT120I GGNCC 4 cut(s) 89, 1242, 1769, 1975
BmiI GGNNCC 6 cut(s) 333, 513, 1008, 1253, 1642, 1976
BmrFI CCNGG 4 cut(s) 22, 329, 995, 1156
BmrI ACTGGG 1 cut(s) 1005
BmsI GCATC 7 cut(s) 376, 602, 662, 1309, 1672, 2044, 2066
BmuI ACTGGG 1 cut(s) 1005
BpiI GAAGAC 1 cut(s) 939
BpmI CTGGAG 2 cut(s) 4, 1177
Bpu1102I GCTNAGC 2 cut(s) 105, 2296
BpuEI CTTGAG 2 cut(s) 939, 1033
BpuMI CCSGG 2 cut(s) 329, 995
BsaAI YACGTR 2 cut(s) 2096, 2194
BsaJI CCNNGG 3 cut(s) 36, 347, 675
BsaWI WCCGGW 1 cut(s) 571
Bse118I RCCGGY 1 cut(s) 443
Bse1I ACTGG 7 cut(s) 249, 419, 1011, 1245, 1314, 1453, 1772
Bse21I CCTNAGG 1 cut(s) 1979
Bse3DI GCAATG 1 cut(s) 341
BseBI CCWGG 2 cut(s) 22, 1156
BseDI CCNNGG 3 cut(s) 36, 347, 675
BseGI GGATG 7 cut(s) 211, 681, 685, 1086, 1381, 1708, 2313
BseMI GCAATG 1 cut(s) 341
BseMII CTCAG 5 cut(s) 96, 531, 1224, 1993, 2036
BseNI ACTGG 7 cut(s) 249, 419, 1011, 1245, 1314, 1453, 1772
BseRI GAGGAG 1 cut(s) 1298
BseSI GKGCMC 2 cut(s) 1256, 1643
BseXI GCAGC 3 cut(s) 294, 1035, 1309
Bsh1236I CGCG 2 cut(s) 1017, 1633
BshFI GGCC 7 cut(s) 35, 41, 443, 447, 568, 1036, 1061
BshNI GGYRCC 2 cut(s) 1251, 1640
BsiHKAI GWGCWC 3 cut(s) 111, 1057, 1340
BsiSI CCGG 6 cut(s) 255, 328, 444, 572, 995, 1058
BslFI GGGAC 2 cut(s) 1720, 1988
BsmAI GTCTC 5 cut(s) 9, 299, 515, 836, 1997
BsmBI CGTCTC 1 cut(s) 515
BsmFI GGGAC 2 cut(s) 1720, 1988
BsmI GAATGC 1 cut(s) 2262
BsnI GGCC 7 cut(s) 35, 41, 443, 447, 568, 1036, 1061
Bsp1286I GDGCHC 5 cut(s) 111, 1057, 1256, 1340, 1643
Bsp1407I TGTACA 2 cut(s) 874, 1728
Bsp143I GATC 8 cut(s) 331, 910, 1006, 1414, 1675, 1798, 1858, 1946
Bsp1720I GCTNAGC 2 cut(s) 105, 2296
Bsp19I CCATGG 1 cut(s) 675
BspACI CCGC 6 cut(s) 208, 319, 998, 1603, 1815, 1864
BspANI GGCC 7 cut(s) 35, 41, 443, 447, 568, 1036, 1061
BspCNI CTCAG 5 cut(s) 97, 530, 1225, 1992, 2037
BspFNI CGCG 2 cut(s) 1017, 1633
BspLI GGNNCC 6 cut(s) 333, 513, 1008, 1253, 1642, 1976
BspMAI CTGCAG 1 cut(s) 1299
BspMI ACCTGC 1 cut(s) 249
BspPI GGATC 6 cut(s) 326, 339, 1001, 1014, 1670, 1954
BspT107I GGYRCC 2 cut(s) 1251, 1640
BsrDI GCAATG 1 cut(s) 341
BsrFI RCCGGY 1 cut(s) 443
BsrGI TGTACA 2 cut(s) 874, 1728
BsrI ACTGG 7 cut(s) 249, 419, 1011, 1245, 1314, 1453, 1772
BssAI RCCGGY 1 cut(s) 443
BssECI CCNNGG 3 cut(s) 36, 347, 675
BssMI GATC 8 cut(s) 331, 910, 1006, 1414, 1675, 1798, 1858, 1946
BssNAI GTATAC 2 cut(s) 622, 2099
BssSI CACGAG 3 cut(s) 299, 1713, 2007
BssT1I CCWWGG 3 cut(s) 36, 347, 675
Bst1107I GTATAC 2 cut(s) 622, 2099
Bst2BI CACGAG 3 cut(s) 299, 1713, 2007
Bst2UI CCWGG 2 cut(s) 22, 1156
Bst4CI ACNGT 4 cut(s) 127, 1733, 1840, 2212
Bst6I CTCTTC 1 cut(s) 1025
BstAPI GCANNNNNTGC 1 cut(s) 409
BstAUI TGTACA 2 cut(s) 874, 1728
BstBAI YACGTR 2 cut(s) 2096, 2194
BstC8I GCNNGC 5 cut(s) 33, 445, 498, 1664, 1764
BstDEI CTNAG 8 cut(s) 105, 517, 824, 1233, 1811, 1979, 2045, 2296
BstDSI CCRYGG 1 cut(s) 675
BstF5I GGATG 7 cut(s) 211, 681, 685, 1086, 1381, 1708, 2313
BstFNI CGCG 2 cut(s) 1017, 1633
BstH2I RGCGCY 1 cut(s) 1196
BstHHI GCGC 2 cut(s) 1195, 1473
BstKTI GATC 8 cut(s) 334, 913, 1009, 1417, 1678, 1801, 1861, 1949
BstMAI GTCTC 5 cut(s) 9, 299, 515, 836, 1997
BstMBI GATC 8 cut(s) 331, 910, 1006, 1414, 1675, 1798, 1858, 1946
BstMWI GCNNNNNNNGC 6 cut(s) 316, 409, 599, 956, 1023, 1192
BstNI CCWGG 2 cut(s) 22, 1156
BstNSI RCATGY 6 cut(s) 247, 745, 1666, 1729, 1766, 1782
BstSCI CCNGG 4 cut(s) 20, 327, 993, 1154
BstSFI CTRYAG 2 cut(s) 1295, 1350
BstSLI GKGCMC 2 cut(s) 1256, 1643
BstSNI TACGTA 1 cut(s) 2096
BstUI CGCG 2 cut(s) 1017, 1633
BstV1I GCAGC 3 cut(s) 294, 1035, 1309
BstV2I GAAGAC 1 cut(s) 939
BstX2I RGATCY 2 cut(s) 331, 1006
BstYI RGATCY 2 cut(s) 331, 1006
BstZ17I GTATAC 2 cut(s) 622, 2099
Bsu36I CCTNAGG 1 cut(s) 1979
BsuRI GGCC 7 cut(s) 35, 41, 443, 447, 568, 1036, 1061
BtgI CCRYGG 1 cut(s) 675
BtgZI GCGATG 1 cut(s) 630
BtsCI GGATG 7 cut(s) 211, 681, 685, 1086, 1381, 1708, 2313
BtsIMutI CAGTG 4 cut(s) 1252, 1424, 1779, 2208
BveI ACCTGC 1 cut(s) 249
Cac8I GCNNGC 5 cut(s) 33, 445, 498, 1664, 1764
CaiI CAGNNNCTG 1 cut(s) 31
CfoI GCGC 2 cut(s) 1195, 1473
Cfr10I RCCGGY 1 cut(s) 443
Cfr13I GGNCC 4 cut(s) 89, 1242, 1769, 1975
CpoI CGGWCCG 1 cut(s) 89
CseI GACGC 2 cut(s) 532, 1006
Csp6I GTAC 3 cut(s) 875, 1729, 2341
CspCI CAANNNNNGTGG 2 cut(s) 2049, 2084
CspI CGGWCCG 1 cut(s) 89
CviQI GTAC 3 cut(s) 875, 1729, 2341
DdeI CTNAG 8 cut(s) 105, 517, 824, 1233, 1811, 1979, 2045, 2296
DpnI GATC 8 cut(s) 333, 912, 1008, 1416, 1677, 1800, 1860, 1948
DpnII GATC 8 cut(s) 331, 910, 1006, 1414, 1675, 1798, 1858, 1946
DraIII CACNNNGTG 1 cut(s) 2197
DrdI GACNNNNNNGTC 2 cut(s) 529, 941
DseDI GACNNNNNNGTC 2 cut(s) 529, 941
EaeI YGGCCR 3 cut(s) 33, 445, 1059
Eam1104I CTCTTC 1 cut(s) 1025
EarI CTCTTC 1 cut(s) 1025
EciI GGCGGA 3 cut(s) 197, 1013, 1618
Ecl136II GAGCTC 2 cut(s) 109, 1055
Eco105I TACGTA 1 cut(s) 2096
Eco130I CCWWGG 3 cut(s) 36, 347, 675
Eco24I GRGCYC 2 cut(s) 111, 1057
Eco32I GATATC 2 cut(s) 1357, 1381
Eco47I GGWCC 4 cut(s) 89, 1242, 1769, 1975
Eco47III AGCGCT 1 cut(s) 1194
Eco53kI GAGCTC 2 cut(s) 109, 1055
Eco57I CTGAAG 1 cut(s) 679
Eco81I CCTNAGG 1 cut(s) 1979
EcoICRI GAGCTC 2 cut(s) 109, 1055
EcoO109I RGGNCCY 1 cut(s) 1975
EcoRII CCWGG 2 cut(s) 20, 1154
EcoRV GATATC 2 cut(s) 1357, 1381
EcoT14I CCWWGG 3 cut(s) 36, 347, 675
EcoT22I ATGCAT 5 cut(s) 157, 1595, 1664, 1764, 2059
EcoT38I GRGCYC 2 cut(s) 111, 1057
ErhI CCWWGG 3 cut(s) 36, 347, 675
Esp3I CGTCTC 1 cut(s) 515
FalI AAGNNNNNCTT 2 cut(s) 948, 980
FaqI GGGAC 2 cut(s) 1720, 1988
FauI CCCGC 2 cut(s) 312, 1857
FauNDI CATATG 2 cut(s) 1369, 1595
FbaI TGATCA 3 cut(s) 1414, 1798, 1858
FblI GTMKAC 3 cut(s) 621, 2098, 2278
Fnu4HI GCNGC 3 cut(s) 308, 1024, 1298
FokI GGATG 7 cut(s) 198, 668, 672, 1073, 1388, 1715, 2320
FriOI GRGCYC 2 cut(s) 111, 1057
FseI GGCCGGCC 1 cut(s) 447
Fsp4HI GCNGC 3 cut(s) 308, 1024, 1298
FspBI CTAG 5 cut(s) 233, 795, 1187, 1322, 2268
GlaI GCGC 2 cut(s) 1194, 1472
GluI GCNGC 3 cut(s) 308, 1024, 1298
GsuI CTGGAG 2 cut(s) 4, 1177
HaeII RGCGCY 1 cut(s) 1196
HaeIII GGCC 7 cut(s) 35, 41, 443, 447, 568, 1036, 1061
HapII CCGG 6 cut(s) 255, 328, 444, 572, 995, 1058
HgaI GACGC 2 cut(s) 532, 1006
HhaI GCGC 2 cut(s) 1195, 1473
Hin6I GCGC 2 cut(s) 1193, 1471
HinP1I GCGC 2 cut(s) 1193, 1471
HincII GTYRAC 5 cut(s) 534, 1171, 1540, 1736, 2279
HindII GTYRAC 5 cut(s) 534, 1171, 1540, 1736, 2279
HindIII AAGCTT 4 cut(s) 227, 725, 957, 2062
HinfI GANTC 8 cut(s) 84, 146, 324, 461, 761, 1065, 2014, 2104
HpaII CCGG 6 cut(s) 255, 328, 444, 572, 995, 1058
HphI GGTGA 4 cut(s) 1469, 1922, 2129, 2314
Hpy166II GTNNAC 7 cut(s) 534, 622, 1171, 1540, 1736, 2099, 2279
Hpy188I TCNGA 8 cut(s) 93, 336, 520, 669, 1234, 1858, 2109, 2348
Hpy8I GTNNAC 7 cut(s) 534, 622, 1171, 1540, 1736, 2099, 2279
Hpy99I CGWCG 2 cut(s) 1022, 1145
HpyAV CCTTC 7 cut(s) 367, 699, 703, 1355, 2138, 2226, 2308
HpyCH4III ACNGT 4 cut(s) 127, 1733, 1840, 2212
HpyCH4IV ACGT 5 cut(s) 238, 1132, 2095, 2193, 2351
HpyF10VI GCNNNNNNNGC 6 cut(s) 316, 409, 599, 956, 1023, 1192
HpyF3I CTNAG 8 cut(s) 105, 517, 824, 1233, 1811, 1979, 2045, 2296
HpySE526I ACGT 5 cut(s) 238, 1132, 2095, 2193, 2351
HspAI GCGC 2 cut(s) 1193, 1471
KroI GCCGGC 1 cut(s) 443
KroNI GCCGGC 1 cut(s) 445
Ksp22I TGATCA 3 cut(s) 1414, 1798, 1858
Kzo9I GATC 8 cut(s) 331, 910, 1006, 1414, 1675, 1798, 1858, 1946
LmnI GCTCC 4 cut(s) 28, 210, 1060, 1335
Lsp1109I GCAGC 3 cut(s) 294, 1035, 1309
LweI GCATC 7 cut(s) 376, 602, 662, 1309, 1672, 2044, 2066
MaeI CTAG 5 cut(s) 233, 795, 1187, 1322, 2268
MaeII ACGT 5 cut(s) 238, 1132, 2095, 2193, 2351
MaeIII GTNAC 3 cut(s) 699, 1667, 1774
MalI GATC 8 cut(s) 333, 912, 1008, 1416, 1677, 1800, 1860, 1948
MboI GATC 8 cut(s) 331, 910, 1006, 1414, 1675, 1798, 1858, 1946
MboII GAAGA 9 cut(s) 7, 376, 944, 1042, 1093, 1111, 1441, 1812, 2323
MfeI CAATTG 1 cut(s) 2253
MflI RGATCY 2 cut(s) 331, 1006
MhlI GDGCHC 5 cut(s) 111, 1057, 1256, 1340, 1643
MlsI TGGCCA 1 cut(s) 35
MluCI AATT 4 cut(s) 466, 1330, 1874, 2253
MluNI TGGCCA 1 cut(s) 35
MlyI GAGTC 4 cut(s) 140, 333, 455, 2023
MmeI TCCRAC 2 cut(s) 489, 624
Mox20I TGGCCA 1 cut(s) 35
Mph1103I ATGCAT 5 cut(s) 157, 1595, 1664, 1764, 2059
MroNI GCCGGC 1 cut(s) 443
MroXI GAANNNNTTC 1 cut(s) 2236
MscI TGGCCA 1 cut(s) 35
MseI TTAA 4 cut(s) 393, 785, 1755, 1877
MslI CAYNNNNRTG 4 cut(s) 746, 945, 1370, 1783
Msp20I TGGCCA 1 cut(s) 35
MspA1I CMGCKG 3 cut(s) 319, 1864, 1936
MspI CCGG 6 cut(s) 255, 328, 444, 572, 995, 1058
MspR9I CCNGG 4 cut(s) 22, 329, 995, 1156
MunI CAATTG 1 cut(s) 2253
Mva1269I GAATGC 1 cut(s) 2262
MvaI CCWGG 2 cut(s) 22, 1156
MvnI CGCG 2 cut(s) 1017, 1633
MwoI GCNNNNNNNGC 6 cut(s) 316, 409, 599, 956, 1023, 1192
NaeI GCCGGC 1 cut(s) 445
NciI CCSGG 2 cut(s) 329, 995
NcoI CCATGG 1 cut(s) 675
NdeI CATATG 2 cut(s) 1369, 1595
NdeII GATC 8 cut(s) 331, 910, 1006, 1414, 1675, 1798, 1858, 1946
NgoMIV GCCGGC 1 cut(s) 443
NlaIV GGNNCC 6 cut(s) 333, 513, 1008, 1253, 1642, 1976
NmuCI GTSAC 1 cut(s) 1774
NsiI ATGCAT 5 cut(s) 157, 1595, 1664, 1764, 2059
NspI RCATGY 6 cut(s) 247, 745, 1666, 1729, 1766, 1782
OliI CACNNNNGTG 1 cut(s) 1370
PaeI GCATGC 2 cut(s) 1666, 1766
PciI ACATGT 1 cut(s) 1725
PcsI WCGNNNNNNNCGW 1 cut(s) 1119
PctI GAATGC 1 cut(s) 2262
PdiI GCCGGC 1 cut(s) 445
PdmI GAANNNNTTC 1 cut(s) 2236
PfeI GAWTC 4 cut(s) 84, 761, 1065, 2104
PfoI TCCNGGA 1 cut(s) 327
PkrI GCNGC 3 cut(s) 309, 1025, 1299
PleI GAGTC 4 cut(s) 140, 332, 455, 2022
PpsI GAGTC 4 cut(s) 140, 332, 455, 2022
Ppu21I YACGTR 2 cut(s) 2096, 2194
PpuMI RGGWCCY 1 cut(s) 1975
PscI ACATGT 1 cut(s) 1725
Psp124BI GAGCTC 2 cut(s) 111, 1057
Psp5II RGGWCCY 1 cut(s) 1975
Psp6I CCWGG 2 cut(s) 20, 1154
PspGI CCWGG 2 cut(s) 20, 1154
PspN4I GGNNCC 6 cut(s) 333, 513, 1008, 1253, 1642, 1976
PspPI GGNCC 4 cut(s) 89, 1242, 1769, 1975
PspPPI RGGWCCY 1 cut(s) 1975
PstI CTGCAG 1 cut(s) 1299
PstNI CAGNNNCTG 1 cut(s) 31
PsuI RGATCY 2 cut(s) 331, 1006
PvuII CAGCTG 1 cut(s) 1936
RigI GGCCGGCC 1 cut(s) 447
RsaI GTAC 3 cut(s) 876, 1730, 2342
RsaNI GTAC 3 cut(s) 875, 1729, 2341
RseI CAYNNNNRTG 4 cut(s) 746, 945, 1370, 1783
Rsr2I CGGWCCG 1 cut(s) 89
RsrII CGGWCCG 1 cut(s) 89
SacI GAGCTC 2 cut(s) 111, 1057
SalI GTCGAC 1 cut(s) 2277
SaqAI TTAA 4 cut(s) 393, 785, 1755, 1877
SatI GCNGC 3 cut(s) 308, 1024, 1298
Sau3AI GATC 8 cut(s) 331, 910, 1006, 1414, 1675, 1798, 1858, 1946
Sau96I GGNCC 4 cut(s) 89, 1242, 1769, 1975
SchI GAGTC 4 cut(s) 140, 333, 455, 2023
ScrFI CCNGG 4 cut(s) 22, 329, 995, 1156
SduI GDGCHC 5 cut(s) 111, 1057, 1256, 1340, 1643
SfaNI GCATC 7 cut(s) 376, 602, 662, 1309, 1672, 2044, 2066
SfcI CTRYAG 2 cut(s) 1295, 1350
SinI GGWCC 4 cut(s) 89, 1242, 1769, 1975
SmiMI CAYNNNNRTG 4 cut(s) 746, 945, 1370, 1783
SmlI CTYRAG 2 cut(s) 954, 1048
SmoI CTYRAG 2 cut(s) 954, 1048
SnaBI TACGTA 1 cut(s) 2096
SphI GCATGC 2 cut(s) 1666, 1766
Sse9I AATT 4 cut(s) 466, 1330, 1874, 2253
SsiI CCGC 6 cut(s) 208, 319, 998, 1603, 1815, 1864
SspI AATATT 2 cut(s) 140, 398
SspMI CTAG 5 cut(s) 233, 795, 1187, 1322, 2268
SstI GAGCTC 2 cut(s) 111, 1057
StyD4I CCNGG 4 cut(s) 20, 327, 993, 1154
StyI CCWWGG 3 cut(s) 36, 347, 675
TaaI ACNGT 4 cut(s) 127, 1733, 1840, 2212
TaiI ACGT 5 cut(s) 241, 1135, 2098, 2196, 2354
TaqI TCGA 8 cut(s) 282, 502, 1077, 1113, 1122, 1327, 1359, 2278
TaqII GACCGA 1 cut(s) 77
TasI AATT 4 cut(s) 466, 1330, 1874, 2253
TatI WGTACW 3 cut(s) 874, 1728, 2340
TfiI GAWTC 4 cut(s) 84, 761, 1065, 2104
Tru1I TTAA 4 cut(s) 393, 785, 1755, 1877
Tru9I TTAA 4 cut(s) 393, 785, 1755, 1877
TscAI CASTG 4 cut(s) 1252, 1424, 1779, 2215
TseFI GTSAC 1 cut(s) 1774
TseI GCWGC 3 cut(s) 307, 1023, 1297
Tsp45I GTSAC 1 cut(s) 1774
TspDTI ATGAA 5 cut(s) 167, 268, 377, 903, 2324
TspGWI ACGGA 1 cut(s) 1129
TspRI CASTG 4 cut(s) 1252, 1424, 1779, 2215
VpaK11BI GGWCC 4 cut(s) 89, 1242, 1769, 1975
XceI RCATGY 6 cut(s) 247, 745, 1666, 1729, 1766, 1782
XcmI CCANNNNNNNNNTGG 2 cut(s) 28, 1940
XmiI GTMKAC 3 cut(s) 621, 2098, 2278
XmnI GAANNNNTTC 1 cut(s) 2236
XspI CTAG 5 cut(s) 233, 795, 1187, 1322, 2268
Zsp2I ATGCAT 5 cut(s) 157, 1595, 1664, 1764, 2059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.