Rmu_sc0004339.1_g000032

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004339.1
OG0004856
Physical Location & Seq
Reverse (-)
120665 .. 123374
2710 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1332 bp
atggacaaaaccaaagctggtttagaaggatctgaatttggagaagcaaaagttaagcatcagaagaatatggactactatttgaaggatataatgggagggatcaaagtattcaagggagagaagttaacgagcttgatggtttcgcagtcacgattctgcagactcaaactgttagcagctgcagccacaaccatgttgctactctgcgcctgcgctgtgcagtacaccacgctcagcgatacaatgaagccacgcgtgctgatatttcgttcttctcaacattttgctcctccacagagtagtactgctgatattgaagtccgcatattgaaagttctgccccagaagcagagaagaagaatagaaatggcttccctctattccatgccccctgctagttggtctaatatgacgttttactacaacagggttcttcctcagataaagaagtatgaagtactgcatttcactaaaactgatacaagacttgccaataatgggatccctgtggaagttcagaaaatgcgctgcaaagcaaattatgaagccctgaggttcactcccccaattgaggaattggggaagaagattgtaaggattctgagagaaaggggcccgttcttagttcttcatctcagatatgaaatggacatgttagccttttcgggttgtactgaaggctgcgatgacaaagaaatcgaagaattgacaaatatgagatatgcttatccatggtggaaagagaaagtaattaattccaccaaaaaaagaaaatctgggaaatgcccattaactcccgaggagactgctcttgcactgagggcattggatatcgatcctagtatccaagtatacatagctgcaggtgatatttatgggggagaaaaacgaatggcatctctgagattagcctttccacacatagttaagaaagaaacattgctggaagcctcagaccttgagcccttccgaaaccattcgaaccaaatggctgcattggattacgttgtgtcactggaaagtgatatcttcgctccaacttatggaggaaacatggcaagagttgtcgaaggccatagacggtacttaggtttcaggacaacaattcgtctggacagaatgcttctagtgaatttgatagaccagtacaagaagggtagactgagctgggatgaattttcccaggcagtgaaaacaggacatgccgatcgcatgggtagccctacacaaagattggagattcctggtaaacccaaggaagaggaatacttctataacaatccacaggaatgtttaccaccaatttctacaaaaccaaagaggctgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

443

Amino Acids

50.8

Weight (kDa)

9.4

Isoelectric Point (pI)

45.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 108 - 364 2.8e-69 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 857
Acc36I ACCTGC 1 cut(s) 857
AccB7I CCANNNNNTGG 1 cut(s) 1046
AccI GTMKAC 2 cut(s) 855, 1162
AccII CGCG 1 cut(s) 258
AciI CCGC 1 cut(s) 325
AclWI GGATC 5 cut(s) 37, 110, 499, 512, 833
AcsI RAATTY 3 cut(s) 35, 1135, 1178
AcuI CTGAAG 1 cut(s) 699
AfaI GTAC 6 cut(s) 227, 307, 462, 676, 1088, 1151
AfiI CCNNNNNNNGG 3 cut(s) 501, 574, 1046
AflIII ACRYGT 2 cut(s) 256, 654
AgsI TTSAA 4 cut(s) 85, 115, 320, 334
AjnI CCWGG 2 cut(s) 1185, 1246
AluBI AGCT 5 cut(s) 17, 135, 182, 863, 1170
AluI AGCT 5 cut(s) 17, 135, 182, 863, 1170
Alw26I GTCTC 1 cut(s) 800
AlwI GGATC 5 cut(s) 37, 110, 499, 512, 833
Ama87I CYCGRG 1 cut(s) 800
AoxI GGCC 2 cut(s) 616, 1075
ApaI GGGCCC 1 cut(s) 620
ApeKI GCWGC 7 cut(s) 179, 182, 185, 531, 684, 863, 995
ApoI RAATTY 3 cut(s) 35, 1135, 1178
AseI ATTAAT 1 cut(s) 756
Asp700I GAANNNNTTC 1 cut(s) 979
AspLEI GCGC 3 cut(s) 212, 218, 531
AspS9I GGNCC 2 cut(s) 616, 617
AsuHPI GGTGA 1 cut(s) 881
AsuII TTCGAA 1 cut(s) 983
AvaI CYCGRG 1 cut(s) 800
AxyI CCTNAGG 1 cut(s) 554
BaeGI GKGCMC 1 cut(s) 620
BaeI ACNNNNGTAYC 2 cut(s) 1078, 1111
BamHI GGATCC 1 cut(s) 504
BanII GRGCYC 2 cut(s) 620, 969
BbvI GCAGC 7 cut(s) 169, 191, 197, 518, 671, 850, 982
BccI CCATC 1 cut(s) 133
BcgI CGANNNNNNTGC 2 cut(s) 791, 825
BciT130I CCWGG 2 cut(s) 1187, 1248
BciVI GTATCC 1 cut(s) 857
BcoDI GTCTC 1 cut(s) 800
BfaI CTAG 3 cut(s) 399, 843, 1130
BfmI CTRYAG 3 cut(s) 160, 183, 864
BfuAI ACCTGC 1 cut(s) 857
BfuI GTATCC 1 cut(s) 857
BisI GCNGC 7 cut(s) 180, 183, 186, 532, 685, 864, 996
BlpI GCTNAGC 1 cut(s) 236
BlsI GCNGC 7 cut(s) 181, 184, 187, 533, 686, 865, 997
BmcAI AGTACT 2 cut(s) 307, 462
Bme1390I CCNGG 2 cut(s) 1187, 1248
BmeT110I CYCGRG 1 cut(s) 800
BmgT120I GGNCC 2 cut(s) 616, 617
BmiI GGNNCC 3 cut(s) 506, 617, 618
BmrFI CCNGG 2 cut(s) 1187, 1248
BmsI GCATC 2 cut(s) 67, 908
BplI GAGNNNNNCTC 2 cut(s) 547, 579
Bpu1102I GCTNAGC 1 cut(s) 236
Bpu14I TTCGAA 1 cut(s) 983
BpuEI CTTGAG 1 cut(s) 983
Bsa29I ATCGAT 1 cut(s) 837
BsaBI GATNNNNATC 1 cut(s) 837
BsaJI CCNNGG 4 cut(s) 734, 801, 1185, 1257
Bsc4I CCNNNNNNNGG 3 cut(s) 501, 574, 1046
Bse1I ACTGG 2 cut(s) 1023, 1147
Bse21I CCTNAGG 1 cut(s) 554
Bse3DI GCAATG 1 cut(s) 941
Bse8I GATNNNNATC 1 cut(s) 837
BseBI CCWGG 2 cut(s) 1187, 1248
BseCI ATCGAT 1 cut(s) 837
BseDI CCNNGG 4 cut(s) 734, 801, 1185, 1257
BseGI GGATG 1 cut(s) 1180
BseJI GATNNNNATC 1 cut(s) 837
BseLI CCNNNNNNNGG 3 cut(s) 501, 574, 1046
BseMI GCAATG 1 cut(s) 941
BseMII CTCAG 9 cut(s) 250, 455, 545, 596, 652, 812, 896, 969, 1157
BseNI ACTGG 2 cut(s) 1023, 1147
BseRI GAGGAG 2 cut(s) 282, 818
BseSI GKGCMC 1 cut(s) 620
BseXI GCAGC 7 cut(s) 169, 191, 197, 518, 671, 850, 982
BseYI CCCAGC 1 cut(s) 1170
BsgI GTGCAG 1 cut(s) 242
Bsh1236I CGCG 1 cut(s) 258
Bsh1285I CGRYCG 1 cut(s) 1213
BshFI GGCC 2 cut(s) 618, 1077
BshVI ATCGAT 1 cut(s) 837
BsiEI CGRYCG 1 cut(s) 1213
BsiHKCI CYCGRG 1 cut(s) 800
BslI CCNNNNNNNGG 3 cut(s) 501, 574, 1046
BsmAI GTCTC 1 cut(s) 800
BsmI GAATGC 1 cut(s) 1128
BsnI GGCC 2 cut(s) 618, 1077
BsoBI CYCGRG 1 cut(s) 800
Bsp119I TTCGAA 1 cut(s) 983
Bsp120I GGGCCC 1 cut(s) 616
Bsp1286I GDGCHC 2 cut(s) 620, 969
Bsp143I GATC 5 cut(s) 29, 102, 504, 838, 1210
Bsp1720I GCTNAGC 1 cut(s) 236
Bsp19I CCATGG 1 cut(s) 734
BspACI CCGC 1 cut(s) 325
BspANI GGCC 2 cut(s) 618, 1077
BspCNI CTCAG 9 cut(s) 249, 454, 546, 597, 651, 813, 897, 968, 1158
BspDI ATCGAT 1 cut(s) 837
BspFNI CGCG 1 cut(s) 258
BspLI GGNNCC 3 cut(s) 506, 617, 618
BspMAI CTGCAG 3 cut(s) 164, 187, 868
BspMI ACCTGC 1 cut(s) 857
BspPI GGATC 5 cut(s) 37, 110, 499, 512, 833
BspT104I TTCGAA 1 cut(s) 983
BsrDI GCAATG 1 cut(s) 941
BsrI ACTGG 2 cut(s) 1023, 1147
BssECI CCNNGG 4 cut(s) 734, 801, 1185, 1257
BssMI GATC 5 cut(s) 29, 102, 504, 838, 1210
BssNAI GTATAC 1 cut(s) 856
BssT1I CCWWGG 2 cut(s) 734, 1257
Bst1107I GTATAC 1 cut(s) 856
Bst2UI CCWGG 2 cut(s) 1187, 1248
Bst4CI ACNGT 2 cut(s) 174, 1086
Bst6I CTCTTC 1 cut(s) 1257
BstBI TTCGAA 1 cut(s) 983
BstC8I GCNNGC 2 cut(s) 214, 260
BstDSI CCRYGG 1 cut(s) 734
BstF5I GGATG 1 cut(s) 1180
BstFNI CGCG 1 cut(s) 258
BstHHI GCGC 3 cut(s) 212, 218, 531
BstKTI GATC 5 cut(s) 32, 105, 507, 841, 1213
BstMAI GTCTC 1 cut(s) 800
BstMBI GATC 5 cut(s) 29, 102, 504, 838, 1210
BstMCI CGRYCG 1 cut(s) 1213
BstMWI GCNNNNNNNGC 5 cut(s) 185, 259, 349, 824, 1221
BstNI CCWGG 2 cut(s) 1187, 1248
BstNSI RCATGY 2 cut(s) 658, 1208
BstSCI CCNGG 2 cut(s) 1185, 1246
BstSFI CTRYAG 3 cut(s) 160, 183, 864
BstSLI GKGCMC 1 cut(s) 620
BstUI CGCG 1 cut(s) 258
BstV1I GCAGC 7 cut(s) 169, 191, 197, 518, 671, 850, 982
BstX2I RGATCY 2 cut(s) 29, 504
BstYI RGATCY 2 cut(s) 29, 504
BstZ17I GTATAC 1 cut(s) 856
Bsu15I ATCGAT 1 cut(s) 837
Bsu36I CCTNAGG 1 cut(s) 554
BsuI GTATCC 1 cut(s) 857
BsuRI GGCC 2 cut(s) 618, 1077
BsuTUI ATCGAT 1 cut(s) 837
BtgI CCRYGG 1 cut(s) 734
BtgZI GCGATG 1 cut(s) 702
BtsCI GGATG 1 cut(s) 1180
BtsI GCAGTG 1 cut(s) 1197
BtsIMutI CAGTG 3 cut(s) 818, 1016, 1197
BveI ACCTGC 1 cut(s) 857
Cac8I GCNNGC 2 cut(s) 214, 260
CfoI GCGC 3 cut(s) 212, 218, 531
Cfr13I GGNCC 2 cut(s) 616, 617
ClaI ATCGAT 1 cut(s) 837
Csp6I GTAC 6 cut(s) 226, 306, 461, 675, 1087, 1150
CviAII CATG 7 cut(s) 196, 388, 655, 735, 1057, 1205, 1216
CviQI GTAC 6 cut(s) 226, 306, 461, 675, 1087, 1150
DpnI GATC 5 cut(s) 31, 104, 506, 840, 1212
DpnII GATC 5 cut(s) 29, 102, 504, 838, 1210
Eam1104I CTCTTC 1 cut(s) 1257
EarI CTCTTC 1 cut(s) 1257
Eco130I CCWWGG 2 cut(s) 734, 1257
Eco24I GRGCYC 2 cut(s) 620, 969
Eco32I GATATC 2 cut(s) 835, 1030
Eco57I CTGAAG 1 cut(s) 699
Eco81I CCTNAGG 1 cut(s) 554
Eco88I CYCGRG 1 cut(s) 800
EcoO109I RGGNCCY 1 cut(s) 616
EcoRII CCWGG 2 cut(s) 1185, 1246
EcoRV GATATC 2 cut(s) 835, 1030
EcoT14I CCWWGG 2 cut(s) 734, 1257
EcoT38I GRGCYC 2 cut(s) 620, 969
ErhI CCWWGG 2 cut(s) 734, 1257
FaeI CATG 7 cut(s) 199, 391, 658, 738, 1060, 1208, 1219
FatI CATG 7 cut(s) 195, 387, 654, 734, 1056, 1204, 1215
FblI GTMKAC 2 cut(s) 855, 1162
Fnu4HI GCNGC 7 cut(s) 180, 183, 186, 532, 685, 864, 996
FokI GGATG 1 cut(s) 1187
FriOI GRGCYC 2 cut(s) 620, 969
Fsp4HI GCNGC 7 cut(s) 180, 183, 186, 532, 685, 864, 996
FspBI CTAG 3 cut(s) 399, 843, 1130
GlaI GCGC 3 cut(s) 211, 217, 530
GluI GCNGC 7 cut(s) 180, 183, 186, 532, 685, 864, 996
GsaI CCCAGC 1 cut(s) 1174
HaeIII GGCC 2 cut(s) 618, 1077
HhaI GCGC 3 cut(s) 212, 218, 531
Hin1II CATG 7 cut(s) 199, 391, 658, 738, 1060, 1208, 1219
Hin6I GCGC 3 cut(s) 210, 216, 529
HinP1I GCGC 3 cut(s) 210, 216, 529
HincII GTYRAC 1 cut(s) 129
HindII GTYRAC 1 cut(s) 129
HinfI GANTC 4 cut(s) 156, 165, 601, 1243
HpaI GTTAAC 1 cut(s) 129
HphI GGTGA 1 cut(s) 881
Hpy166II GTNNAC 7 cut(s) 129, 228, 561, 856, 1163, 1253, 1298
Hpy188I TCNGA 9 cut(s) 34, 63, 444, 522, 606, 641, 906, 958, 974
Hpy188III TCNNGA 4 cut(s) 153, 800, 1099, 1115
Hpy8I GTNNAC 7 cut(s) 129, 228, 561, 856, 1163, 1253, 1298
HpyAV CCTTC 6 cut(s) 20, 79, 674, 979, 1067, 1150
HpyCH4III ACNGT 2 cut(s) 174, 1086
HpyCH4IV ACGT 2 cut(s) 416, 1008
HpyCH4V TGCA 8 cut(s) 162, 185, 223, 466, 534, 818, 866, 998
HpyF10VI GCNNNNNNNGC 5 cut(s) 185, 259, 349, 824, 1221
HpySE526I ACGT 2 cut(s) 416, 1008
Hsp92II CATG 7 cut(s) 199, 391, 658, 738, 1060, 1208, 1219
HspAI GCGC 3 cut(s) 210, 216, 529
KspAI GTTAAC 1 cut(s) 129
Kzo9I GATC 5 cut(s) 29, 102, 504, 838, 1210
LmnI GCTCC 2 cut(s) 295, 1042
Lsp1109I GCAGC 7 cut(s) 169, 191, 197, 518, 671, 850, 982
LweI GCATC 2 cut(s) 67, 908
MaeI CTAG 3 cut(s) 399, 843, 1130
MaeII ACGT 2 cut(s) 416, 1008
MaeIII GTNAC 2 cut(s) 150, 1014
MalI GATC 5 cut(s) 31, 104, 506, 840, 1212
MboI GATC 5 cut(s) 29, 102, 504, 838, 1210
MfeI CAATTG 1 cut(s) 570
MflI RGATCY 2 cut(s) 29, 504
MhlI GDGCHC 2 cut(s) 620, 969
MluI ACGCGT 1 cut(s) 256
MlyI GAGTC 1 cut(s) 159
MmeI TCCRAC 1 cut(s) 1064
MroXI GAANNNNTTC 1 cut(s) 979
MseI TTAA 5 cut(s) 54, 128, 756, 794, 930
MslI CAYNNNNRTG 2 cut(s) 194, 1291
MspA1I CMGCKG 1 cut(s) 182
MspR9I CCNGG 2 cut(s) 1187, 1248
MunI CAATTG 1 cut(s) 570
Mva1269I GAATGC 1 cut(s) 1128
MvaI CCWGG 2 cut(s) 1187, 1248
MvnI CGCG 1 cut(s) 258
MwoI GCNNNNNNNGC 5 cut(s) 185, 259, 349, 824, 1221
NcoI CCATGG 1 cut(s) 734
NdeII GATC 5 cut(s) 29, 102, 504, 838, 1210
NlaIII CATG 7 cut(s) 199, 391, 658, 738, 1060, 1208, 1219
NlaIV GGNNCC 3 cut(s) 506, 617, 618
NmuCI GTSAC 2 cut(s) 150, 1014
NspI RCATGY 2 cut(s) 658, 1208
NspV TTCGAA 1 cut(s) 983
PaqCI CACCTGC 1 cut(s) 857
PciI ACATGT 1 cut(s) 654
PctI GAATGC 1 cut(s) 1128
PdmI GAANNNNTTC 1 cut(s) 979
PfeI GAWTC 3 cut(s) 156, 601, 1243
PflMI CCANNNNNTGG 1 cut(s) 1046
PkrI GCNGC 7 cut(s) 181, 184, 187, 533, 686, 865, 997
Ple19I CGATCG 1 cut(s) 1213
PleI GAGTC 1 cut(s) 159
PpsI GAGTC 1 cut(s) 159
PscI ACATGT 1 cut(s) 654
PshBI ATTAAT 1 cut(s) 756
Psp6I CCWGG 2 cut(s) 1185, 1246
PspFI CCCAGC 1 cut(s) 1170
PspGI CCWGG 2 cut(s) 1185, 1246
PspN4I GGNNCC 3 cut(s) 506, 617, 618
PspOMI GGGCCC 1 cut(s) 616
PspPI GGNCC 2 cut(s) 616, 617
PstI CTGCAG 3 cut(s) 164, 187, 868
PsuI RGATCY 2 cut(s) 29, 504
PvuI CGATCG 1 cut(s) 1213
PvuII CAGCTG 1 cut(s) 182
RsaI GTAC 6 cut(s) 227, 307, 462, 676, 1088, 1151
RsaNI GTAC 6 cut(s) 226, 306, 461, 675, 1087, 1150
RseI CAYNNNNRTG 2 cut(s) 194, 1291
SaqAI TTAA 5 cut(s) 54, 128, 756, 794, 930
SatI GCNGC 7 cut(s) 180, 183, 186, 532, 685, 864, 996
Sau3AI GATC 5 cut(s) 29, 102, 504, 838, 1210
Sau96I GGNCC 2 cut(s) 616, 617
ScaI AGTACT 2 cut(s) 307, 462
SchI GAGTC 1 cut(s) 159
ScrFI CCNGG 2 cut(s) 1187, 1248
SduI GDGCHC 2 cut(s) 620, 969
SfaNI GCATC 2 cut(s) 67, 908
SfcI CTRYAG 3 cut(s) 160, 183, 864
SfuI TTCGAA 1 cut(s) 983
SmiMI CAYNNNNRTG 2 cut(s) 194, 1291
SmlI CTYRAG 1 cut(s) 962
SmoI CTYRAG 1 cut(s) 962
SsiI CCGC 1 cut(s) 325
SspMI CTAG 3 cut(s) 399, 843, 1130
StyD4I CCNGG 2 cut(s) 1185, 1246
StyI CCWWGG 2 cut(s) 734, 1257
TaaI ACNGT 2 cut(s) 174, 1086
TaiI ACGT 2 cut(s) 419, 1011
TaqI TCGA 4 cut(s) 702, 837, 983, 1071
TatI WGTACW 5 cut(s) 225, 305, 460, 674, 1149
TfiI GAWTC 3 cut(s) 156, 601, 1243
Tru1I TTAA 5 cut(s) 54, 128, 756, 794, 930
Tru9I TTAA 5 cut(s) 54, 128, 756, 794, 930
TscAI CASTG 3 cut(s) 825, 1023, 1197
TseFI GTSAC 2 cut(s) 150, 1014
TseI GCWGC 7 cut(s) 179, 182, 185, 531, 684, 863, 995
Tsp45I GTSAC 2 cut(s) 150, 1014
TspDTI ATGAA 6 cut(s) 263, 471, 561, 623, 660, 1191
TspRI CASTG 3 cut(s) 825, 1023, 1197
Van91I CCANNNNNTGG 1 cut(s) 1046
VspI ATTAAT 1 cut(s) 756
XapI RAATTY 3 cut(s) 35, 1135, 1178
XceI RCATGY 2 cut(s) 658, 1208
XmiI GTMKAC 2 cut(s) 855, 1162
XmnI GAANNNNTTC 1 cut(s) 979
XspI CTAG 3 cut(s) 399, 843, 1130
ZrmI AGTACT 2 cut(s) 307, 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.