Rmu_sc0004449.1_g000002

Protein NRDE2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004449.1
OG0010115
Physical Location & Seq
Forward (+)
1852 .. 6943
5092 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2832 bp
atggacgacgacaggccgtcgccgtcgctgttcccagtcatagccgcctctcagcaagtctcaaacgtccctcaatggctctccaacaccagcttcaccaccaacctctccgtcataaacgacgccgttgcctcgcacttcaaacctgagccaccgccaccgccggaggagcaagacgacgtacggccccagccgaagccgttcgagcttctggaatcttctagcggatccgaagcgagcgatgagagagacaggacgagcaagaaggagaaggggaagaggaggaagaagaggcggcgcgcgcagagagacggcgggtcgttcgccggtttcgggtcgagaaagtccagtgttcgggcttgggccgaatccgagactagaccgtcggaaaattattacttggattccagtggcgaccttgataatttggcctttggatgtctctataggatggacattgctcgatacaaaccttatgctgctctttcggatagctccgggaattttaaagctttgtatcaggggaatcagacaatttcaacattggatagagatgctgatgttgatgcattggatggtaaactaaaatccgggggtcgctattggtctgcaaagtacatggctttggaacgccataagaacttaaagcgccttcgtttacttgcccctagagaccttgcggatacagtttcaggtgattttattcctttgacggatattcaaacatccgatgaaggtgaaggtgttgctgctgatgagtcactctcgggaactccagtagtggtcgaagaatcatgggaagatgaattgctacgcaagactcgggagtttaacaaattgacgagggagcatcctcatgatgagaaggtttggttggcttttgctgagttccaagacaaggttgctgatatgcagccccagaaaggtgctcgcttgcagactctggaaaaaaagattagcatattggagaaagccacagagcttaacccagataatgaagaactgttgctttgtcttctaaaggcttataaaagcagagacagttccgatgtattaattagtagatgggaaaagatactcatacagcattcaggaaattaccacttgtggagagagtttctgcatgttattcaaggggagttctccaggttcaaggtttcagacatgagaaaaatgtacgcacatgcaatccaggctatatctgctgcatgcagaatgcactctaggcaggtttgccaaggagaaaaatcttctccagatcccgctattgttcaactagaacttggtctggtggatatttttctcagttattgtaggtttgaatggcaggttggctaccaagagttggccaccgctctatttcaggctgaaattgaattcagtttgttttgtccttctttgctcctcactgagcagagtaagcagatattatttgagcacttctggaacagtgatggtgctagagttggagaagaaggcgctcttggttggtccacatggttggagaaagaggaagaaaataggcaaagggtcatcagagaggagatggcacatgataatgaaggtggttggacaggttggtctgaaccactatcaaaaaataaggaaggtagtaccaatccagaaaaggaagctgaaagtaatgctgtacttgaggaatttcaagaagaaactgagaacgaagatgtcaagcaagaagatgatactgaagctttgctaaagatgctcggtattgatgttgatgttggggctactggtgaggttaaagacgcttcaacctgggttagatggtcagaagaagaaaaatcaagagattgtgatcaatggatgcctgttcgtgcaaaatctgaagctgttgcctctaataatggtgggacacctgatagagaagcagaagaacacctttcgagagtcataatgtatgaagatgttgctgagtacctcttttctttgagctcatttgaggcccgtttatctctagtgttacagttcgttgatttctttggtgggaagacatctcagtggatatctacaaacagttcagcttggtctgagaaactccttagcctagaggcattgccacattcagtattacacactctgaaaagggttcatgatgtcttaagcaaaacacaaggtagttcaaatagtttcagcttggaatctctgttggggaccaccaatgacatccatggacaggcagacctgatgaagtttctttgtaatgctaccttgctctgtttatctgcttttccacgtaattatctcctagaggaagctgctttagttgcagaagagctatctgttgtgaatctgaatccatcccgcagttcagttaccccctgccgtgctctagcaaagcatcttttaaagagtgatcgtcaggatatattgctatgtggtgtttatgcaagaagagaggctttttatgggaatattgatcatgcaagaagagtatttgacatggcgttgtcatctattgaagggcttcctttggaattgaggtccaatgctcctcttctgtatttctggtatgctgaggtggagctagccaaagatcagggaaatcggtctgaatcatcatttcgtgcaatgcatattttatcttgctttggaagtggtggatcatatagtccgtttaaatgtcagccatcaaatttgcaattgctgagagcacgccaaggcttcaaagaaataataagaacagtgcaaatgtcatgtttacgtggtgttatagatgatcaatccgctgctctcgtatgttgtgctgctttatttgaagagttaacttctggatgggctttgggaattgaagttctagatcaggcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NRDE-2

Protein Analysis

943

Amino Acids

106.21

Weight (kDa)

5.08

Isoelectric Point (pI)

50.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NRDE-2 PF08424 273 - 670 1.8e-76 NRDE-2, necessary for RNA interference
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010115)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17712 AT3G17712 AT3G17712 AT3G17740
fragaria_vesca FvH4_5g17940 FvH4_5g17940 FvH4_5g17940
malus_domestica MD14G1242200.v1.1
prunus_persica Prupe.5G239800_v2.0.a1
pyrus_communis pycom14g20360
rosa_chinensis RchiOBHm_Chr7g0177661
rosa_laevigata RLG00000005516
rosa_multiflora Rmu_sc0004449.1_g000002 Rmu_ssc0000236.1_g000002
rosa_roxburghii Rroxscaffold_3G00275680
rosa_rugosa Rorug06G0408300
rosa_samantha Rh7AG007800 Rh7BG007700 Rh7CG008200 Rh7DG007900
rosa_wichuraiana Rw7G000650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1029
AasI GACNNNNNNGTC 2 cut(s) 382, 1579
Acc36I ACCTGC 2 cut(s) 1219, 1318
AccB7I CCANNNNNTGG 1 cut(s) 1345
AccBSI CCGCTC 1 cut(s) 1355
AccII CGCG 2 cut(s) 300, 302
AclWI GGATC 4 cut(s) 222, 235, 1253, 2632
AcoI YGGCCR 1 cut(s) 1347
AcsI RAATTY 4 cut(s) 502, 1376, 1658, 2656
AcuI CTGAAG 2 cut(s) 1728, 1869
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 6 cut(s) 183, 617, 1178, 1615, 1650, 1940
AfiI CCNNNNNNNGG 6 cut(s) 333, 354, 898, 923, 1345, 2197
AflII CTTAAG 1 cut(s) 2122
AhdI GACNNNNNGTC 2 cut(s) 16, 316
AjnI CCWGG 3 cut(s) 1145, 1191, 1778
AjuI GAANNNNNNNTTGG 8 cut(s) 77, 109, 857, 889, 1314, 1346, 1467, 1499
AloI GAACNNNNNNTCC 2 cut(s) 2799, 2831
Alw21I GWGCWC 5 cut(s) 931, 1440, 1958, 2353, 2677
Alw26I GTCTC 7 cut(s) 64, 243, 303, 368, 446, 666, 1032
AlwI GGATC 4 cut(s) 222, 235, 1253, 2632
AlwNI CAGNNNCTG 1 cut(s) 943
Ama87I CYCGRG 2 cut(s) 766, 822
AoxI GGCC 6 cut(s) 14, 185, 363, 429, 1347, 1965
ApeKI GCWGC 7 cut(s) 479, 749, 913, 1205, 2279, 2750, 2768
ApoI RAATTY 4 cut(s) 502, 1376, 1658, 2656
AseI ATTAAT 1 cut(s) 1055
Asp700I GAANNNNTTC 2 cut(s) 200, 2487
AspLEI GCGC 5 cut(s) 300, 302, 304, 651, 1481
AspS9I GGNCC 6 cut(s) 186, 363, 1491, 1966, 2175, 2505
AsuC2I CCSGG 2 cut(s) 499, 592
AsuHPI GGTGA 4 cut(s) 88, 707, 749, 1769
AsuNHI GCTAGC 1 cut(s) 2548
AvaI CYCGRG 2 cut(s) 766, 822
AvaII GGWCC 3 cut(s) 1491, 2175, 2505
BalI TGGCCA 1 cut(s) 1349
BamHI GGATCC 1 cut(s) 227
BanII GRGCYC 1 cut(s) 1958
BbsI GAAGAC 2 cut(s) 1007, 2018
Bbv12I GWGCWC 5 cut(s) 931, 1440, 1958, 2353, 2677
BbvCI CCTCAGC 1 cut(s) 2538
BbvI GCAGC 7 cut(s) 466, 736, 925, 1192, 2266, 2737, 2755
BccI CCATC 9 cut(s) 445, 569, 1059, 1448, 1540, 1782, 2329, 2659, 2790
BceAI ACGGC 6 cut(s) 7, 110, 184, 200, 328, 2331
BcgI CGANNNNNNTGC 2 cut(s) 110, 144
BciT130I CCWGG 3 cut(s) 1147, 1193, 1780
BciVI GTATCC 1 cut(s) 676
BclI TGATCA 3 cut(s) 1819, 2440, 2740
BcnI CCSGG 2 cut(s) 499, 592
BcoDI GTCTC 7 cut(s) 64, 243, 303, 368, 446, 666, 1032
BfmI CTRYAG 1 cut(s) 445
BfoI RGCGCY 2 cut(s) 652, 1482
BfrI CTTAAG 1 cut(s) 2122
BfuAI ACCTGC 2 cut(s) 1219, 1318
BfuI GTATCC 1 cut(s) 676
BisI GCNGC 9 cut(s) 45, 296, 480, 750, 914, 1206, 2280, 2751, 2769
BlsI GCNGC 9 cut(s) 46, 297, 481, 751, 915, 1207, 2281, 2752, 2770
Bme1390I CCNGG 5 cut(s) 499, 592, 1147, 1193, 1780
Bme18I GGWCC 3 cut(s) 1491, 2175, 2505
BmeRI GACNNNNNGTC 2 cut(s) 16, 316
BmeT110I CYCGRG 2 cut(s) 766, 822
BmgT120I GGNCC 6 cut(s) 186, 363, 1491, 1966, 2175, 2505
BmiI GGNNCC 3 cut(s) 188, 229, 2176
BmrFI CCNGG 5 cut(s) 499, 592, 1147, 1193, 1780
BmrI ACTGGG 1 cut(s) 29
BmsI GCATC 6 cut(s) 544, 556, 859, 1713, 1818, 2371
BmtI GCTAGC 1 cut(s) 2552
BmuI ACTGGG 1 cut(s) 29
BpiI GAAGAC 2 cut(s) 1007, 2018
BplI GAGNNNNNCTC 2 cut(s) 749, 781
BpmI CTGGAG 3 cut(s) 759, 1129, 1239
Bpu10I CCTNAGC 3 cut(s) 147, 2063, 2538
BpuEI CTTGAG 1 cut(s) 1673
BpuMI CCSGG 2 cut(s) 499, 592
BsaAI YACGTR 2 cut(s) 2258, 2726
BsaBI GATNNNNATC 1 cut(s) 1818
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 666
BsaJI CCNNGG 5 cut(s) 591, 1237, 1779, 2191, 2680
Bsc4I CCNNNNNNNGG 6 cut(s) 333, 354, 898, 923, 1345, 2197
Bse118I RCCGGY 1 cut(s) 326
Bse1I ACTGG 5 cut(s) 35, 348, 408, 776, 1759
Bse3DI GCAATG 3 cut(s) 456, 2075, 2598
Bse8I GATNNNNATC 1 cut(s) 1818
BseBI CCWGG 3 cut(s) 1147, 1193, 1780
BseDI CCNNGG 5 cut(s) 591, 1237, 1779, 2191, 2680
BseGI GGATG 9 cut(s) 443, 456, 580, 725, 850, 1833, 2187, 2321, 2801
BseJI GATNNNNATC 1 cut(s) 1818
BseLI CCNNNNNNNGG 6 cut(s) 333, 354, 898, 923, 1345, 2197
BseMI GCAATG 3 cut(s) 456, 2075, 2598
BseNI ACTGG 5 cut(s) 35, 348, 408, 776, 1759
BsePI GCGCGC 2 cut(s) 298, 300
BseRI GAGGAG 5 cut(s) 182, 295, 1394, 1556, 2505
BseXI GCAGC 7 cut(s) 466, 736, 925, 1192, 2266, 2737, 2755
BseYI CCCAGC 1 cut(s) 189
Bsh1236I CGCG 2 cut(s) 300, 302
BshFI GGCC 6 cut(s) 16, 187, 365, 431, 1349, 1967
BsiHKAI GWGCWC 5 cut(s) 931, 1440, 1958, 2353, 2677
BsiHKCI CYCGRG 2 cut(s) 766, 822
BsiSI CCGG 4 cut(s) 164, 327, 498, 591
BsiWI CGTACG 1 cut(s) 181
BslFI GGGAC 3 cut(s) 53, 1888, 2188
BslI CCNNNNNNNGG 6 cut(s) 333, 354, 898, 923, 1345, 2197
BsmAI GTCTC 7 cut(s) 64, 243, 303, 368, 446, 666, 1032
BsmBI CGTCTC 1 cut(s) 303
BsmFI GGGAC 3 cut(s) 53, 1888, 2188
BsmI GAATGC 2 cut(s) 1087, 1221
BsnI GGCC 6 cut(s) 16, 187, 365, 431, 1349, 1967
Bso31I GGTCTC 1 cut(s) 666
BsoBI CYCGRG 2 cut(s) 766, 822
Bsp1286I GDGCHC 5 cut(s) 931, 1440, 1958, 2353, 2677
Bsp143I GATC 9 cut(s) 227, 1258, 1819, 2377, 2440, 2557, 2624, 2740, 2821
Bsp19I CCATGG 1 cut(s) 2191
BspANI GGCC 6 cut(s) 16, 187, 365, 431, 1349, 1967
BspFNI CGCG 2 cut(s) 300, 302
BspHI TCATGA 2 cut(s) 856, 2113
BspLI GGNNCC 3 cut(s) 188, 229, 2176
BspMI ACCTGC 2 cut(s) 1219, 1318
BspOI GCTAGC 1 cut(s) 2552
BspPI GGATC 4 cut(s) 222, 235, 1253, 2632
BspQI GCTCTTC 1 cut(s) 2289
BspTI CTTAAG 1 cut(s) 2122
BspTNI GGTCTC 1 cut(s) 666
BsrBI CCGCTC 1 cut(s) 1355
BsrDI GCAATG 3 cut(s) 456, 2075, 2598
BsrFI RCCGGY 1 cut(s) 326
BsrI ACTGG 5 cut(s) 35, 348, 408, 776, 1759
BssAI RCCGGY 1 cut(s) 326
BssECI CCNNGG 5 cut(s) 591, 1237, 1779, 2191, 2680
BssHII GCGCGC 2 cut(s) 298, 300
BssMI GATC 9 cut(s) 227, 1258, 1819, 2377, 2440, 2557, 2624, 2740, 2821
BssNI GRCGYC 1 cut(s) 123
BssT1I CCWWGG 3 cut(s) 1237, 2191, 2680
Bst2UI CCWGG 3 cut(s) 1147, 1193, 1780
Bst4CI ACNGT 8 cut(s) 384, 688, 1005, 1043, 1451, 1989, 2039, 2707
Bst6I CTCTTC 7 cut(s) 272, 284, 2289, 2410, 2446, 2523, 2775
BstACI GRCGYC 1 cut(s) 123
BstAFI CTTAAG 1 cut(s) 2122
BstBAI YACGTR 2 cut(s) 2258, 2726
BstC8I GCNNGC 8 cut(s) 238, 300, 302, 931, 935, 1210, 2550, 2677
BstDSI CCRYGG 1 cut(s) 2191
BstF5I GGATG 9 cut(s) 443, 456, 580, 725, 850, 1833, 2187, 2321, 2801
BstFNI CGCG 2 cut(s) 300, 302
BstH2I RGCGCY 2 cut(s) 652, 1482
BstHHI GCGC 5 cut(s) 300, 302, 304, 651, 1481
BstKTI GATC 9 cut(s) 230, 1261, 1822, 2380, 2443, 2560, 2627, 2743, 2824
BstMAI GTCTC 7 cut(s) 64, 243, 303, 368, 446, 666, 1032
BstMBI GATC 9 cut(s) 227, 1258, 1819, 2377, 2440, 2557, 2624, 2740, 2821
BstMWI GCNNNNNNNGC 9 cut(s) 169, 205, 301, 1193, 1202, 1225, 1420, 1723, 2288
BstNI CCWGG 3 cut(s) 1147, 1193, 1780
BstNSI RCATGY 3 cut(s) 1127, 1187, 1212
BstSCI CCNGG 5 cut(s) 497, 590, 1145, 1191, 1778
BstSFI CTRYAG 1 cut(s) 445
BstUI CGCG 2 cut(s) 300, 302
BstV1I GCAGC 7 cut(s) 466, 736, 925, 1192, 2266, 2737, 2755
BstV2I GAAGAC 2 cut(s) 1007, 2018
BstX2I RGATCY 2 cut(s) 227, 1258
BstXI CCANNNNNNTGG 1 cut(s) 1501
BstYI RGATCY 2 cut(s) 227, 1258
BsuI GTATCC 1 cut(s) 676
BsuRI GGCC 6 cut(s) 16, 187, 365, 431, 1349, 1967
BtgI CCRYGG 1 cut(s) 2191
BtgZI GCGATG 1 cut(s) 255
BtsCI GGATG 9 cut(s) 443, 456, 580, 725, 850, 1833, 2187, 2321, 2801
BtsIMutI CAGTG 6 cut(s) 355, 415, 1407, 1456, 2027, 2712
BveI ACCTGC 2 cut(s) 1219, 1318
Cac8I GCNNGC 8 cut(s) 238, 300, 302, 931, 935, 1210, 2550, 2677
CaiI CAGNNNCTG 1 cut(s) 943
CciI TCATGA 2 cut(s) 856, 2113
CfoI GCGC 5 cut(s) 300, 302, 304, 651, 1481
Cfr10I RCCGGY 1 cut(s) 326
Cfr13I GGNCC 6 cut(s) 186, 363, 1491, 1966, 2175, 2505
CseI GACGC 2 cut(s) 131, 1778
Csp6I GTAC 6 cut(s) 182, 616, 1177, 1614, 1649, 1939
CviQI GTAC 6 cut(s) 182, 616, 1177, 1614, 1649, 1939
DpnI GATC 9 cut(s) 229, 1260, 1821, 2379, 2442, 2559, 2626, 2742, 2823
DpnII GATC 9 cut(s) 227, 1258, 1819, 2377, 2440, 2557, 2624, 2740, 2821
DraI TTTAAA 3 cut(s) 508, 2370, 2641
DrdI GACNNNNNNGTC 2 cut(s) 382, 1579
DriI GACNNNNNGTC 2 cut(s) 16, 316
DseDI GACNNNNNNGTC 2 cut(s) 382, 1579
EaeI YGGCCR 1 cut(s) 1347
Eam1104I CTCTTC 7 cut(s) 272, 284, 2289, 2410, 2446, 2523, 2775
Eam1105I GACNNNNNGTC 2 cut(s) 16, 316
EarI CTCTTC 7 cut(s) 272, 284, 2289, 2410, 2446, 2523, 2775
Ecl136II GAGCTC 1 cut(s) 1956
Eco130I CCWWGG 3 cut(s) 1237, 2191, 2680
Eco24I GRGCYC 1 cut(s) 1958
Eco31I GGTCTC 1 cut(s) 666
Eco32I GATATC 1 cut(s) 2028
Eco47I GGWCC 3 cut(s) 1491, 2175, 2505
Eco53kI GAGCTC 1 cut(s) 1956
Eco57I CTGAAG 2 cut(s) 1728, 1869
Eco88I CYCGRG 2 cut(s) 766, 822
EcoICRI GAGCTC 1 cut(s) 1956
EcoRI GAATTC 1 cut(s) 1376
EcoRII CCWGG 3 cut(s) 1145, 1191, 1778
EcoRV GATATC 1 cut(s) 2028
EcoT14I CCWWGG 3 cut(s) 1237, 2191, 2680
EcoT22I ATGCAT 2 cut(s) 571, 2598
EcoT38I GRGCYC 1 cut(s) 1958
ErhI CCWWGG 3 cut(s) 1237, 2191, 2680
Esp3I CGTCTC 1 cut(s) 303
FalI AAGNNNNNCTT 6 cut(s) 1467, 1499, 1887, 1919, 2407, 2439
FaqI GGGAC 3 cut(s) 53, 1888, 2188
FauI CCCGC 3 cut(s) 308, 1270, 2333
FbaI TGATCA 3 cut(s) 1819, 2440, 2740
Fnu4HI GCNGC 9 cut(s) 45, 296, 480, 750, 914, 1206, 2280, 2751, 2769
FokI GGATG 9 cut(s) 450, 463, 587, 712, 837, 1840, 2174, 2308, 2808
FriOI GRGCYC 1 cut(s) 1958
Fsp4HI GCNGC 9 cut(s) 45, 296, 480, 750, 914, 1206, 2280, 2751, 2769
GlaI GCGC 5 cut(s) 299, 301, 303, 650, 1480
GluI GCNGC 9 cut(s) 45, 296, 480, 750, 914, 1206, 2280, 2751, 2769
GsaI CCCAGC 1 cut(s) 193
GsuI CTGGAG 3 cut(s) 759, 1129, 1239
HaeII RGCGCY 2 cut(s) 652, 1482
HaeIII GGCC 6 cut(s) 16, 187, 365, 431, 1349, 1967
HapII CCGG 4 cut(s) 164, 327, 498, 591
HgaI GACGC 2 cut(s) 131, 1778
HhaI GCGC 5 cut(s) 300, 302, 304, 651, 1481
Hin1I GRCGYC 1 cut(s) 123
Hin6I GCGC 5 cut(s) 298, 300, 302, 649, 1479
HinP1I GCGC 5 cut(s) 298, 300, 302, 649, 1479
HincII GTYRAC 1 cut(s) 2787
HindII GTYRAC 1 cut(s) 2787
HindIII AAGCTT 2 cut(s) 510, 1710
HpaI GTTAAC 1 cut(s) 2787
HpaII CCGG 4 cut(s) 164, 327, 498, 591
HphI GGTGA 4 cut(s) 88, 707, 749, 1769
Hpy166II GTNNAC 5 cut(s) 581, 659, 1494, 2723, 2787
Hpy8I GTNNAC 5 cut(s) 581, 659, 1494, 2723, 2787
Hpy99I CGWCG 6 cut(s) 11, 22, 28, 125, 182, 388
HpyCH4III ACNGT 8 cut(s) 384, 688, 1005, 1043, 1451, 1989, 2039, 2707
HpyCH4IV ACGT 4 cut(s) 66, 180, 2257, 2725
HpyF10VI GCNNNNNNNGC 9 cut(s) 169, 205, 301, 1193, 1202, 1225, 1420, 1723, 2288
HpySE526I ACGT 4 cut(s) 66, 180, 2257, 2725
Hsp92I GRCGYC 1 cut(s) 123
HspAI GCGC 5 cut(s) 298, 300, 302, 649, 1479
Ksp22I TGATCA 3 cut(s) 1819, 2440, 2740
KspAI GTTAAC 1 cut(s) 2787
Kzo9I GATC 9 cut(s) 227, 1258, 1819, 2377, 2440, 2557, 2624, 2740, 2821
LguI GCTCTTC 1 cut(s) 2289
LmnI GCTCC 6 cut(s) 169, 500, 847, 1407, 2518, 2545
Lsp1109I GCAGC 7 cut(s) 466, 736, 925, 1192, 2266, 2737, 2755
LweI GCATC 6 cut(s) 544, 556, 859, 1713, 1818, 2371
MaeII ACGT 4 cut(s) 66, 180, 2257, 2725
MaeIII GTNAC 3 cut(s) 759, 1983, 2335
MalI GATC 9 cut(s) 229, 1260, 1821, 2379, 2442, 2559, 2626, 2742, 2823
MbiI CCGCTC 1 cut(s) 1355
MboI GATC 9 cut(s) 227, 1258, 1819, 2377, 2440, 2557, 2624, 2740, 2821
MfeI CAATTG 1 cut(s) 2663
MflI RGATCY 2 cut(s) 227, 1258
MhlI GDGCHC 5 cut(s) 931, 1440, 1958, 2353, 2677
MlsI TGGCCA 1 cut(s) 1349
MluNI TGGCCA 1 cut(s) 1349
MlyI GAGTC 4 cut(s) 767, 814, 934, 1920
MmeI TCCRAC 5 cut(s) 108, 366, 1447, 1482, 1550
Mox20I TGGCCA 1 cut(s) 1349
Mph1103I ATGCAT 2 cut(s) 571, 2598
MroXI GAANNNNTTC 2 cut(s) 200, 2487
MscI TGGCCA 1 cut(s) 1349
MslI CAYNNNNRTG 3 cut(s) 855, 1557, 2020
Msp20I TGGCCA 1 cut(s) 1349
MspA1I CMGCKG 1 cut(s) 2750
MspCI CTTAAG 1 cut(s) 2122
MspI CCGG 4 cut(s) 164, 327, 498, 591
MspR9I CCNGG 5 cut(s) 499, 592, 1147, 1193, 1780
MunI CAATTG 1 cut(s) 2663
Mva1269I GAATGC 2 cut(s) 1087, 1221
MvaI CCWGG 3 cut(s) 1147, 1193, 1780
MvnI CGCG 2 cut(s) 300, 302
MwoI GCNNNNNNNGC 9 cut(s) 169, 205, 301, 1193, 1202, 1225, 1420, 1723, 2288
NciI CCSGG 2 cut(s) 499, 592
NcoI CCATGG 1 cut(s) 2191
NdeII GATC 9 cut(s) 227, 1258, 1819, 2377, 2440, 2557, 2624, 2740, 2821
NheI GCTAGC 1 cut(s) 2548
NlaIV GGNNCC 3 cut(s) 188, 229, 2176
NmuCI GTSAC 1 cut(s) 759
NsiI ATGCAT 2 cut(s) 571, 2598
NspI RCATGY 3 cut(s) 1127, 1187, 1212
PaeI GCATGC 1 cut(s) 1212
PagI TCATGA 2 cut(s) 856, 2113
PauI GCGCGC 2 cut(s) 298, 300
PciSI GCTCTTC 1 cut(s) 2289
PctI GAATGC 2 cut(s) 1087, 1221
PdmI GAANNNNTTC 2 cut(s) 200, 2487
PfeI GAWTC 9 cut(s) 215, 368, 404, 526, 791, 2162, 2311, 2317, 2576
Pfl23II CGTACG 1 cut(s) 181
PflMI CCANNNNNTGG 1 cut(s) 1345
PfoI TCCNGGA 1 cut(s) 497
PkrI GCNGC 9 cut(s) 46, 297, 481, 751, 915, 1207, 2281, 2752, 2770
PleI GAGTC 4 cut(s) 766, 814, 934, 1919
PpsI GAGTC 4 cut(s) 766, 814, 934, 1919
Ppu21I YACGTR 2 cut(s) 2258, 2726
PshBI ATTAAT 1 cut(s) 1055
PsiI TTATAA 1 cut(s) 1029
Psp124BI GAGCTC 1 cut(s) 1958
Psp6I CCWGG 3 cut(s) 1145, 1191, 1778
PspFI CCCAGC 1 cut(s) 189
PspGI CCWGG 3 cut(s) 1145, 1191, 1778
PspLI CGTACG 1 cut(s) 181
PspN4I GGNNCC 3 cut(s) 188, 229, 2176
PspPI GGNCC 6 cut(s) 186, 363, 1491, 1966, 2175, 2505
PstNI CAGNNNCTG 1 cut(s) 943
PsuI RGATCY 2 cut(s) 227, 1258
PteI GCGCGC 2 cut(s) 298, 300
RsaI GTAC 6 cut(s) 183, 617, 1178, 1615, 1650, 1940
RsaNI GTAC 6 cut(s) 182, 616, 1177, 1614, 1649, 1939
RseI CAYNNNNRTG 3 cut(s) 855, 1557, 2020
SacI GAGCTC 1 cut(s) 1958
SapI GCTCTTC 1 cut(s) 2289
SatI GCNGC 9 cut(s) 45, 296, 480, 750, 914, 1206, 2280, 2751, 2769
Sau3AI GATC 9 cut(s) 227, 1258, 1819, 2377, 2440, 2557, 2624, 2740, 2821
Sau96I GGNCC 6 cut(s) 186, 363, 1491, 1966, 2175, 2505
SchI GAGTC 4 cut(s) 767, 814, 934, 1920
ScrFI CCNGG 5 cut(s) 499, 592, 1147, 1193, 1780
SduI GDGCHC 5 cut(s) 931, 1440, 1958, 2353, 2677
SfaNI GCATC 6 cut(s) 544, 556, 859, 1713, 1818, 2371
SfcI CTRYAG 1 cut(s) 445
SinI GGWCC 3 cut(s) 1491, 2175, 2505
SmiMI CAYNNNNRTG 3 cut(s) 855, 1557, 2020
SmlI CTYRAG 2 cut(s) 1652, 2122
SmoI CTYRAG 2 cut(s) 1652, 2122
SphI GCATGC 1 cut(s) 1212
SspI AATATT 1 cut(s) 2437
SstI GAGCTC 1 cut(s) 1958
StyD4I CCNGG 5 cut(s) 497, 590, 1145, 1191, 1778
StyI CCWWGG 3 cut(s) 1237, 2191, 2680
TaaI ACNGT 8 cut(s) 384, 688, 1005, 1043, 1451, 1989, 2039, 2707
TaiI ACGT 4 cut(s) 69, 183, 2260, 2728
TaqI TCGA 5 cut(s) 204, 338, 463, 786, 1907
TaqII GACCGA 1 cut(s) 2559
TatI WGTACW 2 cut(s) 615, 1648
TauI GCSGC 2 cut(s) 47, 298
TfiI GAWTC 9 cut(s) 215, 368, 404, 526, 791, 2162, 2311, 2317, 2576
TscAI CASTG 6 cut(s) 355, 415, 1414, 1456, 2027, 2712
TseFI GTSAC 1 cut(s) 759
TseI GCWGC 7 cut(s) 479, 749, 913, 1205, 2279, 2750, 2768
Tsp45I GTSAC 1 cut(s) 759
TspDTI ATGAA 7 cut(s) 747, 819, 1011, 1575, 1938, 2102, 2225
TspGWI ACGGA 3 cut(s) 100, 728, 2625
TspRI CASTG 6 cut(s) 355, 415, 1414, 1456, 2027, 2712
Van91I CCANNNNNTGG 1 cut(s) 1345
Vha464I CTTAAG 1 cut(s) 2122
VpaK11BI GGWCC 3 cut(s) 1491, 2175, 2505
VspI ATTAAT 1 cut(s) 1055
XapI RAATTY 4 cut(s) 502, 1376, 1658, 2656
XbaI TCTAGA 1 cut(s) 2818
XceI RCATGY 3 cut(s) 1127, 1187, 1212
XmnI GAANNNNTTC 2 cut(s) 200, 2487
Zsp2I ATGCAT 2 cut(s) 571, 2598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.