Rmu_sc0004584.1_g000009
ERF Family

Pectate lyase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004584.1
OG0004361
Physical Location & Seq
Reverse (-)
26679 .. 29491
2813 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1152 bp
atggatatgtggatgttaggcattatggtggatccaggcttgaaagcaaaaatagatggtgggtctataacagagcatgaattggcctttacccttgtttcatttgcttgtctgctctttctaatctcttcattggttgatcagaacactaagaattggcctttaattgctcctttgccgtcttatggaagagggagagaacgccctggagggagatatgtgagcttcatccatggcgatggagttcatgatgtggttatcactggtgagaatgggacaattgatggccaaggagatgtgtggtggaacatgtggaggcaaagaactcttcaattcaccagaccaaatctcattgagttcatcaactctaaaaacattatcatttccaatgtgattttccagaactctccattttggaatattcaccctgtttattccagcaacattgttgtccggtatgtcacaatattggctccagctgactctcccaacacagatggaattgatccagattcaagctctaatgtctgcatagaggactcgtacatatcaactggagatgatcttgtggctgtaaagagtggatgggatgagtatgggattgcttatggtcgccctagttccgacattaccatccgtcggatcagaggatcatccccattttctggaattgcaataggaagtgaaacctctggtggggtggagaatgtatttgccgaacacataaacctttacaacatgggagttggtatccatataaaaacaaacatcggtaggggaggcttcataagaaacataacagtttcaaatgtgttcatagaaaaggcacggaagggtataaagattgcaggagatgttggagaccaccctgatgacaaattcaatccaaatgcactcccagtggtaaagggcatcacattgacagacatctggggtatacaagttctgcaggcaggtctcatacaaggcatcaagaactcccctttcaccggaatctgtctttccaacatcaacctccatggcatgaccgcaacaacgagaacacctccatggaagtgctcagatgtcagcggggctgctcatctggtgagccccatccaatgctccgagttgaccagtgcccaacacgcaacttcatgctccaactactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

383

Amino Acids

41.66

Weight (kDa)

5.92

Isoelectric Point (pI)

40.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 75 - 356 5.2e-40 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 944
AccB7I CCANNNNNTGG 1 cut(s) 665
AccI GTMKAC 1 cut(s) 937
AciI CCGC 2 cut(s) 1029, 1071
AclWI GGATC 5 cut(s) 26, 39, 500, 650, 658
AcoI YGGCCR 1 cut(s) 286
AcsI RAATTY 1 cut(s) 878
AfaI GTAC 1 cut(s) 545
AfiI CCNNNNNNNGG 5 cut(s) 185, 639, 665, 696, 989
AflIII ACRYGT 1 cut(s) 309
AgsI TTSAA 5 cut(s) 43, 332, 516, 807, 883
AjnI CCWGG 2 cut(s) 34, 205
AjuI GAANNNNNNNTTGG 2 cut(s) 380, 412
AluBI AGCT 3 cut(s) 225, 479, 519
AluI AGCT 3 cut(s) 225, 479, 519
Alw21I GWGCWC 1 cut(s) 1061
Alw26I GTCTC 2 cut(s) 855, 962
AlwI GGATC 5 cut(s) 26, 39, 500, 650, 658
AoxI GGCC 3 cut(s) 84, 158, 286
ApeKI GCWGC 1 cut(s) 1076
ApoI RAATTY 1 cut(s) 878
AsuHPI GGTGA 5 cut(s) 278, 328, 416, 979, 1099
BaeGI GKGCMC 1 cut(s) 1123
BalI TGGCCA 1 cut(s) 288
BamHI GGATCC 1 cut(s) 31
BanII GRGCYC 1 cut(s) 1094
Bbv12I GWGCWC 1 cut(s) 1061
BbvI GCAGC 1 cut(s) 1063
BccI CCATC 7 cut(s) 50, 233, 278, 491, 579, 641, 1103
BceAI ACGGC 1 cut(s) 163
BciT130I CCWGG 2 cut(s) 36, 207
BciVI GTATCC 1 cut(s) 761
BclI TGATCA 1 cut(s) 139
BcoDI GTCTC 2 cut(s) 855, 962
BfaI CTAG 1 cut(s) 618
BfmI CTRYAG 1 cut(s) 947
BfuAI ACCTGC 1 cut(s) 944
BfuI GTATCC 1 cut(s) 761
BisI GCNGC 1 cut(s) 1077
BlsI GCNGC 1 cut(s) 1078
Bme1390I CCNGG 2 cut(s) 36, 207
BmiI GGNNCC 2 cut(s) 33, 474
BmrFI CCNGG 2 cut(s) 36, 207
BmrI ACTGGG 1 cut(s) 893
BmsI GCATC 2 cut(s) 921, 978
BmuI ACTGGG 1 cut(s) 893
BplI GAGNNNNNCTC 2 cut(s) 1030, 1062
BpmI CTGGAG 3 cut(s) 228, 459, 576
BsaI GGTCTC 2 cut(s) 855, 962
BsaJI CCNNGG 5 cut(s) 205, 232, 289, 1018, 1049
BsaWI WCCGGW 2 cut(s) 453, 989
Bsc4I CCNNNNNNNGG 5 cut(s) 185, 639, 665, 696, 989
Bse1I ACTGG 4 cut(s) 268, 559, 899, 1116
BseBI CCWGG 2 cut(s) 36, 207
BseDI CCNNGG 5 cut(s) 205, 232, 289, 1018, 1049
BseGI GGATG 7 cut(s) 18, 228, 590, 595, 633, 653, 1095
BseLI CCNNNNNNNGG 5 cut(s) 185, 639, 665, 696, 989
BseMII CTCAG 1 cut(s) 1074
BseNI ACTGG 4 cut(s) 268, 559, 899, 1116
BseSI GKGCMC 1 cut(s) 1123
BseXI GCAGC 1 cut(s) 1063
BshFI GGCC 3 cut(s) 86, 160, 288
BsiHKAI GWGCWC 1 cut(s) 1061
BsiSI CCGG 2 cut(s) 454, 990
BslFI GGGAC 1 cut(s) 289
BslI CCNNNNNNNGG 5 cut(s) 185, 639, 665, 696, 989
BsmAI GTCTC 2 cut(s) 855, 962
BsmFI GGGAC 1 cut(s) 289
BsnI GGCC 3 cut(s) 86, 160, 288
Bso31I GGTCTC 2 cut(s) 855, 962
Bsp1286I GDGCHC 3 cut(s) 1061, 1094, 1123
Bsp143I GATC 6 cut(s) 31, 139, 505, 562, 642, 650
Bsp19I CCATGG 3 cut(s) 232, 1018, 1049
BspACI CCGC 2 cut(s) 1029, 1071
BspANI GGCC 3 cut(s) 86, 160, 288
BspCNI CTCAG 1 cut(s) 1073
BspHI TCATGA 1 cut(s) 247
BspLI GGNNCC 2 cut(s) 33, 474
BspMAI CTGCAG 1 cut(s) 951
BspMI ACCTGC 1 cut(s) 944
BspPI GGATC 5 cut(s) 26, 39, 500, 650, 658
BspTNI GGTCTC 2 cut(s) 855, 962
BsrI ACTGG 4 cut(s) 268, 559, 899, 1116
BssECI CCNNGG 5 cut(s) 205, 232, 289, 1018, 1049
BssMI GATC 6 cut(s) 31, 139, 505, 562, 642, 650
BssNAI GTATAC 1 cut(s) 938
BssT1I CCWWGG 4 cut(s) 232, 289, 1018, 1049
Bst1107I GTATAC 1 cut(s) 938
Bst2UI CCWGG 2 cut(s) 36, 207
Bst4CI ACNGT 1 cut(s) 802
Bst6I CTCTTC 3 cut(s) 133, 184, 333
BstC8I GCNNGC 1 cut(s) 951
BstDEI CTNAG 2 cut(s) 150, 1060
BstDSI CCRYGG 3 cut(s) 232, 1018, 1049
BstF5I GGATG 7 cut(s) 18, 228, 590, 595, 633, 653, 1095
BstKTI GATC 6 cut(s) 34, 142, 508, 565, 645, 653
BstMAI GTCTC 2 cut(s) 855, 962
BstMBI GATC 6 cut(s) 31, 139, 505, 562, 642, 650
BstMWI GCNNNNNNNGC 1 cut(s) 1127
BstNI CCWGG 2 cut(s) 36, 207
BstNSI RCATGY 1 cut(s) 313
BstSCI CCNGG 2 cut(s) 34, 205
BstSFI CTRYAG 1 cut(s) 947
BstSLI GKGCMC 1 cut(s) 1123
BstV1I GCAGC 1 cut(s) 1063
BstX2I RGATCY 1 cut(s) 31
BstXI CCANNNNNNTGG 1 cut(s) 239
BstYI RGATCY 1 cut(s) 31
BstZ17I GTATAC 1 cut(s) 938
BsuI GTATCC 1 cut(s) 761
BsuRI GGCC 3 cut(s) 86, 160, 288
BtgI CCRYGG 3 cut(s) 232, 1018, 1049
BtgZI GCGATG 1 cut(s) 252
BtsCI GGATG 7 cut(s) 18, 228, 590, 595, 633, 653, 1095
BtsIMutI CAGTG 3 cut(s) 261, 906, 1123
BveI ACCTGC 1 cut(s) 944
Cac8I GCNNGC 1 cut(s) 951
CciI TCATGA 1 cut(s) 247
Csp6I GTAC 1 cut(s) 544
CviAII CATG 9 cut(s) 77, 233, 248, 310, 739, 1019, 1024, 1050, 1137
CviQI GTAC 1 cut(s) 544
DdeI CTNAG 2 cut(s) 150, 1060
DpnI GATC 6 cut(s) 33, 141, 507, 564, 644, 652
DpnII GATC 6 cut(s) 31, 139, 505, 562, 642, 650
EaeI YGGCCR 1 cut(s) 286
Eam1104I CTCTTC 3 cut(s) 133, 184, 333
EarI CTCTTC 3 cut(s) 133, 184, 333
Eco130I CCWWGG 4 cut(s) 232, 289, 1018, 1049
Eco24I GRGCYC 1 cut(s) 1094
Eco31I GGTCTC 2 cut(s) 855, 962
EcoRII CCWGG 2 cut(s) 34, 205
EcoT14I CCWWGG 4 cut(s) 232, 289, 1018, 1049
EcoT38I GRGCYC 1 cut(s) 1094
ErhI CCWWGG 4 cut(s) 232, 289, 1018, 1049
FaeI CATG 9 cut(s) 80, 236, 251, 313, 742, 1022, 1027, 1053, 1140
FaqI GGGAC 1 cut(s) 289
FatI CATG 9 cut(s) 76, 232, 247, 309, 738, 1018, 1023, 1049, 1136
FauI CCCGC 1 cut(s) 1064
FbaI TGATCA 1 cut(s) 139
FblI GTMKAC 1 cut(s) 937
Fnu4HI GCNGC 1 cut(s) 1077
FokI GGATG 7 cut(s) 25, 215, 597, 602, 620, 640, 1082
FriOI GRGCYC 1 cut(s) 1094
Fsp4HI GCNGC 1 cut(s) 1077
FspBI CTAG 1 cut(s) 618
GluI GCNGC 1 cut(s) 1077
GsuI CTGGAG 3 cut(s) 228, 459, 576
HaeIII GGCC 3 cut(s) 86, 160, 288
HapII CCGG 2 cut(s) 454, 990
Hin1II CATG 9 cut(s) 80, 236, 251, 313, 742, 1022, 1027, 1053, 1140
HincII GTYRAC 1 cut(s) 1113
HindII GTYRAC 1 cut(s) 1113
HinfI GANTC 4 cut(s) 482, 512, 539, 993
HpaII CCGG 2 cut(s) 454, 990
HphI GGTGA 5 cut(s) 278, 328, 416, 979, 1099
Hpy166II GTNNAC 2 cut(s) 938, 1113
Hpy188I TCNGA 6 cut(s) 144, 625, 642, 647, 1063, 1108
Hpy188III TCNNGA 5 cut(s) 248, 400, 509, 666, 973
Hpy8I GTNNAC 2 cut(s) 938, 1113
Hpy99I CGWCG 1 cut(s) 642
HpyAV CCTTC 1 cut(s) 826
HpyCH4III ACNGT 1 cut(s) 802
HpyCH4V TGCA 5 cut(s) 531, 674, 848, 893, 949
HpyF10VI GCNNNNNNNGC 1 cut(s) 1127
HpyF3I CTNAG 2 cut(s) 150, 1060
Hsp92II CATG 9 cut(s) 80, 236, 251, 313, 742, 1022, 1027, 1053, 1140
Ksp22I TGATCA 1 cut(s) 139
Kzo9I GATC 6 cut(s) 31, 139, 505, 562, 642, 650
LmnI GCTCC 4 cut(s) 175, 478, 1109, 1145
Lsp1109I GCAGC 1 cut(s) 1063
LweI GCATC 2 cut(s) 921, 978
MaeI CTAG 1 cut(s) 618
MaeIII GTNAC 1 cut(s) 460
MalI GATC 6 cut(s) 33, 141, 507, 564, 644, 652
MboI GATC 6 cut(s) 31, 139, 505, 562, 642, 650
MboII GAAGA 3 cut(s) 120, 201, 320
MfeI CAATTG 1 cut(s) 279
MflI RGATCY 1 cut(s) 31
MhlI GDGCHC 3 cut(s) 1061, 1094, 1123
MlsI TGGCCA 1 cut(s) 288
MluCI AATT 8 cut(s) 80, 154, 165, 279, 332, 501, 669, 878
MluNI TGGCCA 1 cut(s) 288
MlyI GAGTC 2 cut(s) 476, 533
MmeI TCCRAC 4 cut(s) 620, 648, 838, 1029
MnlI CCTC 9 cut(s) 185, 203, 309, 529, 641, 700, 773, 1025, 1056
Mox20I TGGCCA 1 cut(s) 288
MscI TGGCCA 1 cut(s) 288
MseI TTAA 1 cut(s) 164
MslI CAYNNNNRTG 5 cut(s) 26, 237, 870, 1048, 1054
Msp20I TGGCCA 1 cut(s) 288
MspA1I CMGCKG 2 cut(s) 479, 1071
MspI CCGG 2 cut(s) 454, 990
MspR9I CCNGG 2 cut(s) 36, 207
MunI CAATTG 1 cut(s) 279
MvaI CCWGG 2 cut(s) 36, 207
MwoI GCNNNNNNNGC 1 cut(s) 1127
NcoI CCATGG 3 cut(s) 232, 1018, 1049
NdeII GATC 6 cut(s) 31, 139, 505, 562, 642, 650
NlaIII CATG 9 cut(s) 80, 236, 251, 313, 742, 1022, 1027, 1053, 1140
NlaIV GGNNCC 2 cut(s) 33, 474
NmuCI GTSAC 1 cut(s) 460
NspI RCATGY 1 cut(s) 313
PagI TCATGA 1 cut(s) 247
PciI ACATGT 1 cut(s) 309
PfeI GAWTC 2 cut(s) 512, 993
PflMI CCANNNNNTGG 1 cut(s) 665
PkrI GCNGC 1 cut(s) 1078
PleI GAGTC 2 cut(s) 476, 533
PpsI GAGTC 2 cut(s) 476, 533
PscI ACATGT 1 cut(s) 309
Psp6I CCWGG 2 cut(s) 34, 205
PspGI CCWGG 2 cut(s) 34, 205
PspN4I GGNNCC 2 cut(s) 33, 474
PstI CTGCAG 1 cut(s) 951
PsuI RGATCY 1 cut(s) 31
PvuII CAGCTG 1 cut(s) 479
RsaI GTAC 1 cut(s) 545
RsaNI GTAC 1 cut(s) 544
RseI CAYNNNNRTG 5 cut(s) 26, 237, 870, 1048, 1054
SaqAI TTAA 1 cut(s) 164
SatI GCNGC 1 cut(s) 1077
Sau3AI GATC 6 cut(s) 31, 139, 505, 562, 642, 650
SchI GAGTC 2 cut(s) 476, 533
ScrFI CCNGG 2 cut(s) 36, 207
SduI GDGCHC 3 cut(s) 1061, 1094, 1123
SetI ASST 8 cut(s) 227, 481, 521, 692, 732, 958, 1017, 1048
SfaNI GCATC 2 cut(s) 921, 978
SfcI CTRYAG 1 cut(s) 947
SmiMI CAYNNNNRTG 5 cut(s) 26, 237, 870, 1048, 1054
Sse9I AATT 8 cut(s) 80, 154, 165, 279, 332, 501, 669, 878
SsiI CCGC 2 cut(s) 1029, 1071
SspI AATATT 2 cut(s) 421, 468
SspMI CTAG 1 cut(s) 618
StyD4I CCNGG 2 cut(s) 34, 205
StyI CCWWGG 4 cut(s) 232, 289, 1018, 1049
TaaI ACNGT 1 cut(s) 802
TasI AATT 8 cut(s) 80, 154, 165, 279, 332, 501, 669, 878
TfiI GAWTC 2 cut(s) 512, 993
Tru1I TTAA 1 cut(s) 164
Tru9I TTAA 1 cut(s) 164
TscAI CASTG 3 cut(s) 268, 906, 1123
TseFI GTSAC 1 cut(s) 460
TseI GCWGC 1 cut(s) 1076
Tsp45I GTSAC 1 cut(s) 460
TspDTI ATGAA 9 cut(s) 90, 93, 120, 217, 236, 349, 775, 805, 1125
TspGWI ACGGA 2 cut(s) 626, 844
TspRI CASTG 3 cut(s) 268, 906, 1123
Van91I CCANNNNNTGG 1 cut(s) 665
XapI RAATTY 1 cut(s) 878
XceI RCATGY 1 cut(s) 313
XmiI GTMKAC 1 cut(s) 937
XspI CTAG 1 cut(s) 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.