Rmu_sc0004657.1_g000019

BEACH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004657.1
OG0002031
Physical Location & Seq
Reverse (-)
61622 .. 108037
46416 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 10401 bp
atggctgacatccattttgagactgaggaggtagggctacagatggactcgagcctcgtggtgttgggggctgcagcgtttcaggatgggaactggtgcatggtgggccgtttgttggcgccgaaagcaaggttgcccggtttcaagggtactatttccacgatttggaatatcagatcaggactgaccattcaggatgcaggggagcgttttgtgttccaatttgagaatgaaggtgtgcgcaaccggatcttgcgtggcggtccctggttttatcggaatactatgctggtggtgggagaatatgatggtctcaatccagtggaggaggtgcctctaaatctgatggaggcctgggaagcacatcaagctgcctccagcatgatccagtcgccagagcttccaactggcagagatggtgttgtcgaggaggaggacaaggtcgcgcaatcagatcaggtttcgaggaggctttgggctcgagctcccagaggacgtcagcgacatcatccacgacccctgcctagtcgaaagactactttcggtcccggtgaagttggagatgaggccgttctaaatatactttgggctagatatcaaaaagctgtcgataaggtggaaaagaggaggctgcttcatgtttttctcaagcagttcctagttgtatacaagaactgggaaccagtaaattctgaccaaaattcagaggctgcttcgactaccatccaacatgcagagtctgatgatgtagttattggttgctttgccggacatcccgcagaagtcatcttagtattgactgaagagattacacaattaactgctatggttgttgaattaaatagcagtacagtccgatcagcagacttctgtggccattccacaatcttaaatatcatttctgaagggattccactccttgatgctcttctgattgttaatcgttcgctgcaaaattgcagagttttgggatactatggtggcattccaaagctaacggcattaatgaaaggggttgttgtccaacttaaaacaataagttgcgcactttcagctgacgaaaaattatctaattttaccgtggagaagacaggactcctgcaacaaatacttgtatatgtggtgtcaataataagtacttttattgatttgaattcaaatgtaaatgagaaatgtcagttgtatagtaataccataggctttgtcacggggggtggtgcatctcctgtcgactcttctagtagttcaaatgctccccctagtgaaatattgttacgttggcatcagagggcagttgtgtctgtgatggaagctggtggacttaattggttagtagagctgctgcgtgtgatccgaagcttaagcatgaaagaacagtggacagatacatcacttctgtatttgtctttgagaattcttcattcaacattatctcagaaccctcgtggtcaaaatctttttaaaagcattggaggacttgaagttctattggatggtcttggaataccatcaaataatgggctaacattgaaaaactctgcttgtgctgctgacaagagatctgagaatcccttgataaaaatcttccagctgcatgttctctccttggaagttctcaaagaggctgtctttgggaatttcagcaacttgcaatttctatgtgaaaatggaagggttcagaaattcgcaaatagcttttgttcccctgctttcacgtttcacgagtataaacaacacaccaagggattgtctggtcagcaagattctcaaatgcctgaagttgactttggaactggcaatactgtgaatagtcgcattgcagaggtttctgttgctcttcctgccgatggttcttactctcaagtttggaatgattatgttcttaagttgagcggagtattttgttctttccttccctcttcagaagatataaaatctcatgatgttgaactgtccactggtcgaattactgtgccattttcttcatcgtatggtgaactttcagtggcatggatcatgagggttcttcaaacagtgttcccctgcattaaggcctgttcagatcaaaatgatttaaaaagccacttaagggtctttgtcactgcgctgcagcactctgtattgaatgcatttaggatggttcttgatgcatcccctgtttcactcaaagtatttcgagacgagggaatctgggagcttatattttctgagaattttttctattttggaccagctcctgatgatctttctggagagtgctgtacatgtattgagtccctgaggaaaaatggaatgctttcttcttctagtgacatcatcagtcaagcaagagtttgtgggattggagttctccaaatggatgtgatttcatttttggagtttgctgcaacttctaatgggagcccagacaattcgcctgaattatctgctcttgtagaagcccttgaacagtgtgcttgcaatcctgatgtttctagtgcacttgccaagtgtttgcttcgcatattaaagctttcagctgagaaaactattgcttcgtttaaagcagttaattcatttcctcgtgtgcttaaggttgtttgcattcaatcccaagagtcaagaaggtctagcaatataatcctttctgttgagagtaatgtcggtgaagtggttccatgtcagcagaagagatctaagtcacatgagacaatgcagagatggatcaaatgcatggaaacatccatggagctttatatggagtttttctctacaatagaagatgctggaagtttggttttgcatagctctgaatgtattggtcggttgtttgatttattctggatagaaggtttgagaaataaagtgctaaagcatattcttgatctcatgaagattgtaccatcatctgaagaagatcaaagagcaaagttgcagttatgttctaaatacgtggaaacattcactcaaataaaggaccgggaaagaaactttgttgcgctatctatcaatttactagccggaatgcaagatatgctccagattgatcctgtgtcctatcagactttgtttcgtgagggagagtgctttttgcacattgtctctttactaaatggtaaccttgatgatgctaatggagagactttggttctgggtgtccttcaaactcttacctgtttgcttgcaagcaatgacacatcaaaggccacatttagaggtcttgcgggcaaggggtaccagacattgcaaagtctattgttggagttttgccagtctcgctcaagtgaaggacttttgaatgcgctgcttgatatgcttgttgatggaaagtttgatatgaaagcaagtcctaaaataaagaatgaagacgtgatcatactttatctcagcgtcctgcagaagagcagtgactcgttgcagcatagtgggcttgatgtgtttcagcaattaatgagggattccatttccaatcgagcttcttgcgtcagagcaggaatgctcgattttctacttgattggttttcacaagaaggtaatgatagtgtcatcttgaaaattgcccaattaattcaagttgttggtgggcatagcacatctgggaaggacattcgcaaaatctttgccctccttcgaagtgagaaaattgggaatcagcacaagtattgctcactgttgttgtccagtgttttgtcaatgctaaaggaaaagggacctactgccttttttgacctcaatgggaatgattctgggatcatgattaaaacacctgttcagtggccactcaacaagggattttctttttcatgttggttgagggtagagaatttccccagaagtgggacaatgggtcttttcagtttccttacagaaaatggaagagggtgcatggcggcactagcgaaggacaagcttctgtatgagtccatcaatctgaagcgacactctgtccagctgcagctaaaaattgtcacaaagaaatggcatttgctatgtataactcatagcataggaagagctttttcagggggtagcttattgaggtgttacgttgatggtgatcttgtttcttctgaaagatgcagttatgcgaaagttaatgacttgctaacaagttgtacagttggcgccaaatttgacgggcccctgtctgatgatgatttatcttcgaaatccataagggattcgcatccttttcttggtcaggttggtcctgtttatctgttcaatgatgccatttcttctgagcaagttcagggcatctgttctcttggaccaagctacatgtattcgttccttgacaatgatgcttcatcatctaatgaaactaagctcggaggggttcttgatgtcaaagatggtcttgcatcgaaaattatttttggacttaatgctcaggcaagtaatggcaggacactatttaatgtttcaccaatgctggatcatgcatcagataggaactcatttgaagcaaccatgatggttggcacgcagcaatgctcgagacgcttactgcaacagataatttactgtgtgggaggggtatcagttttcttccctcttattgcccagtctgaaaaatatgaaaatgaagaaagtgggcaatttaaagatacgtttcctatacctataactagagatcgggtgactgctgaagtcattgagctaatcgcttctgtgcttgatgagaatgtagctaatcaacaacagatgcatcttctctctgggttctcaatactgggattcttgttgcagtcagttcctcctcagcaacttaatttggaaacactttcatctctaaaacatttctatcatatcgttgcaaactgtggcttggctgaactgcttaccaaagaggctatatctagcatatttcttaaccctctcatctggctgtacacggattataaggtgcagcgagaattatacatgtttgtcatctggcagtttgataatgatccaaggatgcttaaaagcctttgccggctcccccgtgttattgatataatacgccaattctattgggataatccaaaatctcgatttgctattggaaacatgcctctcattcatcccatcacaaaacaagttcttggagagagacctagtaatgacgaaatacataaaattcgtcttcttttgttgagccttggcgaattgagccttaggcagaacattgcaggggcagatataagagctctaatagctttctttgagacaagtcaggatatgacctgcattgaagatgttctacatatggttattcgagcgatttctcaaaagccactgcttgcagatttccttgaacaggtcaatttgattggtggctgtcacatatttgccaatcttcttcagagagaacatgaacctatcagattacttagcttgcagctccttggaaagcttttagttgatctcccatctgagaaaaagggagcaagatttttcaatcttgcagtcggaaggtcccgatcgctttctgacggccataaaaaaatctcaaagcaaccaattttttcagccatgtctgatagattatttaggtttccacaaacagataacttgtgtgccactctgtttgatgttcttcttggtggtgctagccctaaacaggtattacagaagcacaacctggctgagaagcagagaagcaaaggacatatgtcacattttctccttcctcaaattttggttcttatatttagattcctgtctggctgtgaggatgcagcttcaagaatgaaaattatcagagatttacttgatcttcttgattcggatccttcaaatgtcgaagcttttatggattttggatggaatgcctggttaacagcttgtgtgaagcttggtgtgttcaaagactacaaagttactccagaagatcaagatcacgatgagaaaacagaacaagatttagtgcggaatatattttgtgttgttctttgttactatgttcattctgtgaaaggtgggtggcaccagttagaagagacggtaactttcctgctaatgcagtgtgaacatggtggagtttcatttcggtacttgcttcgtgacatatataaggatctgatcaggggattgttggaattgtcgtctgaagagagtatttttgtgtcgcaaccgagccgggacaacacattataccttctaagactgatcgatgagatgctcatttctgaaatggaccaaaaccttccgggtggagtttcatttcggtacttgcttcgtgacatatataaggatctgatcaggggattgttggaattgtcgtctgaagagagtatttttgtgtcgcaaccgagccgggacaacacattataccttctaagactgatcgatgagatgctcatttctgaaatggaccaaaaccttccgttcccagcaagcactacagatttctctcttgattcgttagagttagaacgccataaagattatggttctgccttgtttgaagtggtgcatggagaaaatgacagccaaatatcaaggacttctgggagttgcaagcagcctgtcaaaagtgaagatggcattgtcagtgacaagtggtgggatctgtatgacagtttgtggattattattagtgagatgaatggtaagggaccgagcaagtcgttatcgaaatcatcaccaactgcaattccatcttttggtcagagagcacgtggcttagtagaatcgttgaacattcctgctgcagaggtggctgcagttgttgtatcaggagggataggcagtgcattaggtggcaaaccaaacaaaaatgtcgataaggcaatgctcttacgaggagagaggtcttctttagaaagagcatcgcgatgcgtccaactggtcattccacttctgccatttctcttatcagctgatgacgagcaaagtaaaagcagattgcagctgttcatctgggcattgcttgttgttaggtcgcagtttggaatgctagatgacggtgctcgttttcatgttatatcgcacttgattcgagaaactatcagctttggtaaatcaatgcttgctagtagcattatgggtagggaggattccttggattcaggcagcaatgtaaaagaaaccggatccattcacaatctgattcagcgagatcgagtacttgcagcagttgcagaggagttaaaatatataaagtcattagatgctgatcgccaaaggcagctgcatgaactccactccaggatggatgaaaatgcctctgcagaatcaaatgatagaaaggcttttgaagatgagatacaaagtagcttaacctccattcttgctttggatgacagcagaagagctgcattccagctaactcacgaggaggagcagcaaaatgttgctgagaaatggatacacatgtttcgtgctttgattgatgagagaggtccatggtctgcgaacccttttccaaatagtgctgtaaggcattggaaacttgagaagatagaagatacctggcgacggagaccaaaactaagacagaattatcattttgatgaaaagttgtgccaaccttcatcctctgtctccagcattgagattacacctccttttaatgaaagtaaatctgcttttgttggtcacattcctgagcaaatgaagcggtttttgctaaaaggagtatggaagattactgatgaggggagctcagaacccaatgaaactgataccgagctctgtggacagaagccgtccatccacacagatacctcagacagccagacctcagaattagcaaaggacaccagtgattggatacaggagaggaaagattcgtcttccccttcacatgagacagaaactagtgaggttcttacatcagttccttgtgtacttgtatccccaaaaagaaaactagctggacatttggcggtcatgaaaaacgtcttgcatttctttggagaatttttggttgaaggtactggaggatcatccattttcagaaacttccctgcttcaagcaattttgatttgaccaaacctgatcaaaagcagaaatttataaaacaaccaatatgctttggtttggatgcctcgaagggcaccatggttcagaaatttgaggcaataggtgaaaatgttctcaaaagaaaggagctaaaaaatattaaacgccacaggaggtggaacatgggcaagatcaaatctgtttgttggacgcgttatttgcttcggtatactgcaatagagattttcttctgcgattcatctgcaccgatatttttgaattttgcatcacagaaggatgcaaaagatattggaaccttaatagttgctaccaggaacgaatatttgtttccaaaaggaagtggtagggataaaagtggagctatttcatttgttgatagacgtgtatccttggaaatggcagaagctgcccgagagagctggaggagaagggatatgacaaattttgaatatctaatgatccttaatacgctggctggaaggtcttataatgatttgacacaatatcctgtgtttccttgggtcttggctgattattcctcggaggttcttgatttcaacaagtcttcaacctttcgggatctttcaaaaccagttggagcacttgatttaaaacgatttgaggtgtttgaagatagatatcgtagctttactgatcctgatatacctagtttctactatgggtctcattattcaagcatggggatagtgctttattaccttctcagattagagccatttacttctctccatcgcaatttgcagggtggtaaatttgaccatgcagatcgtctctttcaaagcattgagtcaacttatcagaactgcctttctaatacaagtgatgtgaaggagttaatacctgagttcttttatatgccagagtttcttgtgaattcaaactcctatcatttaggggtgaaacaagatggtgaacctattggagatgtttgtctccctccctgggccaagggctcacctgaagaatttgttaacaagaacagagaggcccttgaaagtgaatatgttagctctaatctacaccattggatagatttagtatttggttacaaacagcgtggaaaacctgctgtggaggcagcaaatattttttattacttgacctatgaaggcgctgttgatctggacaccatggaagacgatttacaaagatcagcaattgaagaccaaattgcaaattttggacaaacaccaattcagattttccgtaaaaaacacccaagaagaggaccacccattccaattgctcatcctctgcgctttgcacctagttctattaacatgacttccattgttagtagtgcaagtcaaccaaggttgtcttcattgtatattcgtacagtggactcaaatattgttgttgtaaaccaggggctcaccttgtcagttaagatgtggttgacatcctcactgcaatctggtggaaattttaccttctctggctcccaggatccttcttttggcattggttctgatgtcctttctccgcgtaaaattgggagtccttctgctgaaaatgttgaacttggaccacaatgcttcgcaacaatgcaaacagcatcggagaattttttgatctcatgtggcaattgggaaaatagctttcaggttatatccttgaatgatggcagagtggtgcaaagcatcaggcagcatagggatgtggtcagttgtgttgcagtaacatctgatggacgcttccttgcgactggaagttatgacactacaatcatggtctgggaagttttccgaggtagaacacaagaaaagagaccacgtaatacacagacggaactaccccgtaaagattatgtcattgttgaaactccttttcgtattctgtgcggacacgatgatataattacatgcttatatatcagtgtagagcttgacatagttatcagtggatcgaaagatggaacatgtgtttttcataccctgcagaagggaagatatgtaagatccgtgcgacatccatctggatgtgcgttatcaaagcttgttgcctctcggcatggaaggatagtgttttacgcagatggtgatctcagtttgcatctttattcaattaatggtaaacatcttgcttcttctgaatccaatggccgcctcaactgtgttgagctgagtgggtgtgaggaatttttggtttgtgctggtgaccacgggcaaatagttgtacgctccatgaattcacttgaggtcataaagaaatataatggagctggaaagatcataacatctctaacagtgaccccagaagaatgcttcttagctggaacaaaagacggaacccttcttgtgattgatcattcctttgcacttgaagtcatgcagctaaaaaggcgagcaatgatcaatgatgtaaatcccagcaagatgtgcatctgtcgtaatataacattgttgaagagaagtaccacatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Beach DUF4704 DUF4800 Laminin_G_3 PH_BEACH WD40

Protein Analysis

3466

Amino Acids

387.92

Weight (kDa)

6.1

Isoelectric Point (pI)

45.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 31 - 100 3.2e-08 Domain of unknown function (DUF4283)
Neurobeachin PF20425 804 - 1226 8e-212 Neurobeachin alpha solenoid region
Laminin_G_3 PF13385 1238 - 1411 2.2e-08 Concanavalin A-like lectin/glucanases superfamily
DUF4704 PF15787 1477 - 1995 7.9e-160 Neurobeachin/BDCP, DUF4704 alpha solenoid region
DUF4800 PF16057 2390 - 2496 1e-06 Domain of unknown function (DUF4800)
PH_BEACH PF14844 2691 - 2750 2e-10 PH domain associated with Beige/BEACH
Beach PF02138 2791 - 3067 5.8e-124 Beige/BEACH domain
NBCH_WD40 PF20426 3088 - 3439 1.2e-197 Neurobeachin beta propeller domain
WD40_Prp19 PF24814 3175 - 3352 3.9e-11 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 3175 - 3312 3.6e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 3176 - 3244 1e-07 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 3176 - 3355 3.4e-08 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 3179 - 3245 5.9e-08 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 3181 - 3240 7.9e-08 THOC3 beta-propeller domain
WD40 PF00400 3202 - 3238 1.4e-07 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 3202 - 3356 2.5e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR5 PF25175 3268 - 3418 4e-07 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 3272 - 3428 4.1e-07 THOC3 beta-propeller domain
WDR55 PF24796 3275 - 3426 1.3e-06 WDR55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 4884, 7988, 8442
AatII GACGTC 1 cut(s) 499
Acc16I TGCGCA 2 cut(s) 242, 1045
Acc36I ACCTGC 2 cut(s) 5219, 9049
Acc65I GGTACC 1 cut(s) 3213
AccB1I GGYRCC 6 cut(s) 118, 331, 3213, 4103, 5952, 8027
AccB7I CCANNNNNTGG 3 cut(s) 165, 6632, 7737
AccBSI CCGCTC 1 cut(s) 1893
AccI GTMKAC 3 cut(s) 666, 1230, 8162
AccII CGCG 4 cut(s) 446, 6802, 8146, 9463
AcoI YGGCCR 4 cut(s) 874, 3755, 5461, 10078
AcvI CACGTG 1 cut(s) 6647
AcyI GRCGYC 3 cut(s) 119, 496, 4104
AflII CTTAAG 4 cut(s) 1360, 1883, 2086, 2570
AflIII ACRYGT 7 cut(s) 2264, 4260, 4905, 7341, 8144, 8335, 9896
AhdI GACNNNNNGTC 2 cut(s) 3825, 3923
AhlI ACTAGT 1 cut(s) 7787
AjiI CACGTC 2 cut(s) 3349, 8336
AjuI GAANNNNNNNTTGG 6 cut(s) 1770, 1802, 6804, 6836, 9280, 9312
AloI GAACNNNNNNTCC 2 cut(s) 1467, 1499
Alw21I GWGCWC 8 cut(s) 487, 2482, 5176, 6646, 6940, 7634, 7662, 8557
Alw44I GTGCAC 1 cut(s) 2478
AlwNI CAGNNNCTG 4 cut(s) 710, 740, 2237, 8360
Ama87I CYCGRG 4 cut(s) 49, 480, 4477, 8364
ApaI GGGCCC 1 cut(s) 4122
ApaLI GTGCAC 1 cut(s) 2478
ArsI GACNNNNNNTTYG 5 cut(s) 31, 1611, 1643, 8134, 8166
AseI ATTAAT 4 cut(s) 1004, 3428, 3545, 10044
Asp700I GAANNNNTTC 6 cut(s) 909, 2216, 2296, 2652, 5223, 8064
Asp718I GGTACC 1 cut(s) 3213
AsuC2I CCSGG 6 cut(s) 138, 549, 2960, 6107, 6177, 6284
AsuII TTCGAA 2 cut(s) 3610, 4145
AsuNHI GCTAGC 1 cut(s) 5576
AvaI CYCGRG 4 cut(s) 49, 480, 4477, 8364
AxyI CCTNAGG 2 cut(s) 2279, 5141
BaeGI GKGCMC 3 cut(s) 2482, 4122, 8030
BaeI ACNNNNGTAYC 4 cut(s) 964, 997, 7328, 7361
BalI TGGCCA 2 cut(s) 876, 3757
BamHI GGATCC 3 cut(s) 5755, 7061, 9424
BanI GGYRCC 6 cut(s) 118, 331, 3213, 4103, 5952, 8027
BanII GRGCYC 9 cut(s) 481, 487, 2405, 4122, 5176, 7634, 7662, 8930, 9351
BarI GAAGNNNNNNTAC 6 cut(s) 5211, 5243, 7218, 7250, 9102, 9134
BauI CACGAG 5 cut(s) 56, 1443, 1721, 2562, 7301
BbrPI CACGTG 1 cut(s) 6647
BbsI GAAGAC 9 cut(s) 1094, 3351, 5102, 6774, 7755, 8511, 9117, 9144, 9288
Bbv12I GWGCWC 8 cut(s) 487, 2482, 5176, 6646, 6940, 7634, 7662, 8557
BbvCI CCTCAGC 1 cut(s) 4743
BceAI ACGGC 5 cut(s) 93, 554, 1014, 5476, 7660
BcgI CGANNNNNNTGC 6 cut(s) 3441, 3475, 6947, 6981, 8177, 8211
BciVI GTATCC 5 cut(s) 965, 7329, 7734, 7834, 8350
BclI TGATCA 6 cut(s) 3351, 6048, 6225, 7969, 10282, 10329
BcnI CCSGG 6 cut(s) 138, 549, 2960, 6107, 6177, 6284
BcuI ACTAGT 1 cut(s) 7787
BfoI RGCGCY 3 cut(s) 122, 4107, 9090
BfrI CTTAAG 4 cut(s) 1360, 1883, 2086, 2570
BfuAI ACCTGC 2 cut(s) 5219, 9049
BfuI GTATCC 5 cut(s) 965, 7329, 7734, 7834, 8350
BglII AGATCT 2 cut(s) 1559, 2672
BmcAI AGTACT 2 cut(s) 1138, 7095
BmeRI GACNNNNNGTC 2 cut(s) 3825, 3923
BmeT110I CYCGRG 4 cut(s) 49, 480, 4477, 8364
BmgBI CACGTC 2 cut(s) 3349, 8336
BmrI ACTGGG 3 cut(s) 685, 4540, 4724
BmtI GCTAGC 1 cut(s) 5580
BmuI ACTGGG 3 cut(s) 685, 4540, 4724
BoxI GACNNNNGTC 1 cut(s) 5638
BpiI GAAGAC 9 cut(s) 1094, 3351, 5102, 6774, 7755, 8511, 9117, 9144, 9288
BplI GAGNNNNNCTC 4 cut(s) 2732, 2764, 7616, 7648
BpmI CTGGAG 8 cut(s) 361, 2271, 3002, 5835, 7159, 7498, 7929, 8395
Bpu10I CCTNAGC 3 cut(s) 4371, 4743, 7575
Bpu14I TTCGAA 2 cut(s) 3610, 4145
BpuEI CTTGAG 5 cut(s) 632, 1845, 3244, 7442, 10193
BpuMI CCSGG 6 cut(s) 138, 549, 2960, 6107, 6177, 6284
Bsa29I ATCGAT 2 cut(s) 6138, 6315
BsaAI YACGTR 3 cut(s) 2932, 6647, 9752
BsaHI GRCGYC 3 cut(s) 119, 496, 4104
BsaI GGTCTC 5 cut(s) 317, 5071, 7444, 8643, 9739
BsaWI WCCGGW 2 cut(s) 246, 7058
BsaXI ACNNNNNCTCC 2 cut(s) 5634, 5664
Bse118I RCCGGY 1 cut(s) 4959
Bse21I CCTNAGG 2 cut(s) 2279, 5141
Bse3DI GCAATG 9 cut(s) 1815, 3175, 3221, 4478, 5151, 6765, 6893, 7051, 10332
BseCI ATCGAT 2 cut(s) 6138, 6315
BseMI GCAATG 9 cut(s) 1815, 3175, 3221, 4478, 5151, 6765, 6893, 7051, 10332
BseSI GKGCMC 3 cut(s) 2482, 4122, 8030
BseYI CCCAGC 2 cut(s) 6358, 10346
BsgI GTGCAG 2 cut(s) 4910, 8181
Bsh1236I CGCG 4 cut(s) 446, 6802, 8146, 9463
Bsh1285I CGRYCG 1 cut(s) 5450
BshNI GGYRCC 6 cut(s) 118, 331, 3213, 4103, 5952, 8027
BshVI ATCGAT 2 cut(s) 6138, 6315
BsiEI CGRYCG 1 cut(s) 5450
BsiHKAI GWGCWC 8 cut(s) 487, 2482, 5176, 6646, 6940, 7634, 7662, 8557
BsiHKCI CYCGRG 4 cut(s) 49, 480, 4477, 8364
BslFI GGGAC 9 cut(s) 249, 531, 2260, 3702, 3832, 5428, 6122, 6299, 6597
BsmBI CGTCTC 4 cut(s) 2172, 4474, 5960, 8750
BsmFI GGGAC 9 cut(s) 249, 531, 2260, 3702, 3832, 5428, 6122, 6299, 6597
Bso31I GGTCTC 5 cut(s) 317, 5071, 7444, 8643, 9739
BsoBI CYCGRG 4 cut(s) 49, 480, 4477, 8364
Bsp119I TTCGAA 2 cut(s) 3610, 4145
Bsp120I GGGCCC 1 cut(s) 4118
Bsp1407I TGTACA 3 cut(s) 2261, 4094, 4872
Bsp19I CCATGG 4 cut(s) 2724, 7373, 8031, 9105
Bsp68I TCGCGA 1 cut(s) 6802
BspDI ATCGAT 2 cut(s) 6138, 6315
BspFNI CGCG 4 cut(s) 446, 6802, 8146, 9463
BspHI TCATGA 5 cut(s) 1939, 2016, 2868, 3732, 7860
BspMAI CTGCAG 8 cut(s) 76, 2112, 3378, 3936, 6682, 6694, 7201, 9918
BspMI ACCTGC 2 cut(s) 5219, 9049
BspOI GCTAGC 1 cut(s) 5580
BspQI GCTCTTC 5 cut(s) 933, 1842, 3374, 3985, 7273
BspT104I TTCGAA 2 cut(s) 3610, 4145
BspT107I GGYRCC 6 cut(s) 118, 331, 3213, 4103, 5952, 8027
BspTI CTTAAG 4 cut(s) 1360, 1883, 2086, 2570
BspTNI GGTCTC 5 cut(s) 317, 5071, 7444, 8643, 9739
BsrBI CCGCTC 1 cut(s) 1893
BsrDI GCAATG 9 cut(s) 1815, 3175, 3221, 4478, 5151, 6765, 6893, 7051, 10332
BsrFI RCCGGY 1 cut(s) 4959
BsrGI TGTACA 3 cut(s) 2261, 4094, 4872
BssAI RCCGGY 1 cut(s) 4959
BssNAI GTATAC 2 cut(s) 667, 8163
BssNI GRCGYC 3 cut(s) 119, 496, 4104
BssSI CACGAG 5 cut(s) 56, 1443, 1721, 2562, 7301
Bst1107I GTATAC 2 cut(s) 667, 8163
Bst2BI CACGAG 5 cut(s) 56, 1443, 1721, 2562, 7301
BstACI GRCGYC 3 cut(s) 119, 496, 4104
BstAFI CTTAAG 4 cut(s) 1360, 1883, 2086, 2570
BstAPI GCANNNNNTGC 4 cut(s) 3013, 7106, 8360, 10356
BstAUI TGTACA 3 cut(s) 2261, 4094, 4872
BstBAI YACGTR 3 cut(s) 2932, 6647, 9752
BstBI TTCGAA 2 cut(s) 3610, 4145
BstDSI CCRYGG 6 cut(s) 1080, 2724, 7373, 8031, 9105, 10138
BstEII GGTNACC 2 cut(s) 3095, 10133
BstENI CCTNNNNNAGG 2 cut(s) 5282, 9917
BstFNI CGCG 4 cut(s) 446, 6802, 8146, 9463
BstH2I RGCGCY 3 cut(s) 122, 4107, 9090
BstMCI CGRYCG 1 cut(s) 5450
BstNSI RCATGY 9 cut(s) 734, 1598, 2268, 4264, 4909, 5038, 7345, 9843, 9900
BstPAI GACNNNNGTC 1 cut(s) 5638
BstPI GGTNACC 2 cut(s) 3095, 10133
BstSLI GKGCMC 3 cut(s) 2482, 4122, 8030
BstUI CGCG 4 cut(s) 446, 6802, 8146, 9463
BstV2I GAAGAC 9 cut(s) 1094, 3351, 5102, 6774, 7755, 8511, 9117, 9144, 9288
BstZ17I GTATAC 2 cut(s) 667, 8163
Bsu15I ATCGAT 2 cut(s) 6138, 6315
Bsu36I CCTNAGG 2 cut(s) 2279, 5141
BsuI GTATCC 5 cut(s) 965, 7329, 7734, 7834, 8350
BsuTUI ATCGAT 2 cut(s) 6138, 6315
BtgI CCRYGG 6 cut(s) 1080, 2724, 7373, 8031, 9105, 10138
BtgZI GCGATG 3 cut(s) 6783, 6817, 8690
BtrI CACGTC 2 cut(s) 3349, 8336
BtsI GCAGTG 6 cut(s) 2100, 3391, 5261, 5996, 6724, 9383
BtuMI TCGCGA 1 cut(s) 6802
BveI ACCTGC 2 cut(s) 5219, 9049
CaiI CAGNNNCTG 4 cut(s) 710, 740, 2237, 8360
CciI TCATGA 5 cut(s) 1939, 2016, 2868, 3732, 7860
Cfr10I RCCGGY 1 cut(s) 4959
ClaI ATCGAT 2 cut(s) 6138, 6315
CseI GACGC 6 cut(s) 3358, 3451, 4491, 6796, 8152, 9678
CspCI CAANNNNNGTGG 4 cut(s) 5536, 5571, 9013, 9048
DinI GGCGCC 2 cut(s) 120, 4105
DraI TTTAAA 5 cut(s) 1462, 2076, 2542, 4585, 8565
DriI GACNNNNNGTC 2 cut(s) 3825, 3923
EaeI YGGCCR 4 cut(s) 874, 3755, 5461, 10078
Eam1105I GACNNNNNGTC 2 cut(s) 3825, 3923
Ecl136II GAGCTC 4 cut(s) 485, 5174, 7632, 7660
Eco147I AGGCCT 2 cut(s) 353, 2054
Eco24I GRGCYC 9 cut(s) 481, 487, 2405, 4122, 5176, 7634, 7662, 8930, 9351
Eco31I GGTCTC 5 cut(s) 317, 5071, 7444, 8643, 9739
Eco32I GATATC 2 cut(s) 596, 8594
Eco53kI GAGCTC 4 cut(s) 485, 5174, 7632, 7660
Eco72I CACGTG 1 cut(s) 6647
Eco81I CCTNAGG 2 cut(s) 2279, 5141
Eco88I CYCGRG 4 cut(s) 49, 480, 4477, 8364
Eco91I GGTNACC 2 cut(s) 3095, 10133
EcoICRI GAGCTC 4 cut(s) 485, 5174, 7632, 7660
EcoNI CCTNNNNNAGG 2 cut(s) 5282, 9917
EcoO109I RGGNCCY 4 cut(s) 3689, 4119, 5442, 8962
EcoO65I GGTNACC 2 cut(s) 3095, 10133
EcoRI GAATTC 4 cut(s) 1153, 1413, 8848, 10165
EcoRV GATATC 2 cut(s) 596, 8594
EcoT22I ATGCAT 5 cut(s) 2131, 2152, 2714, 4426, 4692
EcoT38I GRGCYC 9 cut(s) 481, 487, 2405, 4122, 5176, 7634, 7662, 8930, 9351
EgeI GGCGCC 2 cut(s) 120, 4105
EheI GGCGCC 2 cut(s) 120, 4105
Esp3I CGTCTC 4 cut(s) 2172, 4474, 5960, 8750
FalI AAGNNNNNCTT 4 cut(s) 4323, 4355, 9280, 9312
FaqI GGGAC 9 cut(s) 249, 531, 2260, 3702, 3832, 5428, 6122, 6299, 6597
FauI CCCGC 2 cut(s) 784, 3196
FauNDI CATATG 2 cut(s) 5232, 5637
FbaI TGATCA 6 cut(s) 3351, 6048, 6225, 7969, 10282, 10329
FblI GTMKAC 3 cut(s) 666, 1230, 8162
FriOI GRGCYC 9 cut(s) 481, 487, 2405, 4122, 5176, 7634, 7662, 8930, 9351
FspI TGCGCA 2 cut(s) 242, 1045
GsaI CCCAGC 2 cut(s) 6362, 10350
GsuI CTGGAG 8 cut(s) 361, 2271, 3002, 5835, 7159, 7498, 7929, 8395
HaeII RGCGCY 3 cut(s) 122, 4107, 9090
HgaI GACGC 6 cut(s) 3358, 3451, 4491, 6796, 8152, 9678
Hin1I GRCGYC 3 cut(s) 119, 496, 4104
HincII GTYRAC 7 cut(s) 1231, 1783, 5805, 8766, 8947, 9284, 9375
HindII GTYRAC 7 cut(s) 1231, 1783, 5805, 8766, 8947, 9284, 9375
HindIII AAGCTT 7 cut(s) 1357, 2510, 3887, 5378, 5772, 5819, 9971
HpaI GTTAAC 2 cut(s) 5805, 8947
Hpy99I CGWCG 1 cut(s) 7449
Hsp92I GRCGYC 3 cut(s) 119, 496, 4104
KasI GGCGCC 2 cut(s) 118, 4103
KpnI GGTACC 1 cut(s) 3217
KroI GCCGGC 1 cut(s) 4959
KroNI GCCGGC 1 cut(s) 4961
Ksp22I TGATCA 6 cut(s) 3351, 6048, 6225, 7969, 10282, 10329
KspAI GTTAAC 2 cut(s) 5805, 8947
LguI GCTCTTC 5 cut(s) 933, 1842, 3374, 3985, 7273
MbiI CCGCTC 1 cut(s) 1893
MfeI CAATTG 3 cut(s) 9132, 9216, 9562
MlsI TGGCCA 2 cut(s) 876, 3757
MluI ACGCGT 1 cut(s) 8144
MluNI TGGCCA 2 cut(s) 876, 3757
Mly113I GGCGCC 2 cut(s) 119, 4104
Mox20I TGGCCA 2 cut(s) 876, 3757
Mph1103I ATGCAT 5 cut(s) 2131, 2152, 2714, 4426, 4692
MroNI GCCGGC 1 cut(s) 4959
MroXI GAANNNNTTC 6 cut(s) 909, 2216, 2296, 2652, 5223, 8064
MscI TGGCCA 2 cut(s) 876, 3757
MslI CAYNNNNRTG 4 cut(s) 6802, 7479, 9633, 9955
Msp20I TGGCCA 2 cut(s) 876, 3757
MspA1I CMGCKG 7 cut(s) 1055, 1591, 2519, 3931, 6848, 6880, 7159
MspCI CTTAAG 4 cut(s) 1360, 1883, 2086, 2570
MunI CAATTG 3 cut(s) 9132, 9216, 9562
MvnI CGCG 4 cut(s) 446, 6802, 8146, 9463
NaeI GCCGGC 1 cut(s) 4961
NarI GGCGCC 2 cut(s) 119, 4104
NciI CCSGG 6 cut(s) 138, 549, 2960, 6107, 6177, 6284
NcoI CCATGG 4 cut(s) 2724, 7373, 8031, 9105
NdeI CATATG 2 cut(s) 5232, 5637
NgoMIV GCCGGC 1 cut(s) 4959
NheI GCTAGC 1 cut(s) 5576
NmeAIII GCCGAG 1 cut(s) 9964
NruI TCGCGA 1 cut(s) 6802
NsbI TGCGCA 2 cut(s) 242, 1045
NsiI ATGCAT 5 cut(s) 2131, 2152, 2714, 4426, 4692
NspI RCATGY 9 cut(s) 734, 1598, 2268, 4264, 4909, 5038, 7345, 9843, 9900
NspV TTCGAA 2 cut(s) 3610, 4145
PaeR7I CTCGAG 3 cut(s) 49, 480, 4477
PagI TCATGA 5 cut(s) 1939, 2016, 2868, 3732, 7860
PasI CCCWGGG 1 cut(s) 8916
PceI AGGCCT 2 cut(s) 353, 2054
PciI ACATGT 5 cut(s) 2264, 4260, 4905, 7341, 9896
PciSI GCTCTTC 5 cut(s) 933, 1842, 3374, 3985, 7273
PdiI GCCGGC 1 cut(s) 4961
PdmI GAANNNNTTC 6 cut(s) 909, 2216, 2296, 2652, 5223, 8064
PflFI GACNNNGTC 1 cut(s) 440
PflMI CCANNNNNTGG 3 cut(s) 165, 6632, 7737
PfoI TCCNGGA 1 cut(s) 7175
Ple19I CGATCG 1 cut(s) 5450
PluTI GGCGCC 2 cut(s) 122, 4107
PmaCI CACGTG 1 cut(s) 6647
PmlI CACGTG 1 cut(s) 6647
Ppu21I YACGTR 3 cut(s) 2932, 6647, 9752
PpuMI RGGWCCY 2 cut(s) 3689, 5442
PscI ACATGT 5 cut(s) 2264, 4260, 4905, 7341, 9896
PshAI GACNNNNGTC 1 cut(s) 5638
PshBI ATTAAT 4 cut(s) 1004, 3428, 3545, 10044
PsiI TTATAA 3 cut(s) 4884, 7988, 8442
Psp124BI GAGCTC 4 cut(s) 487, 5176, 7634, 7662
Psp5II RGGWCCY 2 cut(s) 3689, 5442
PspCI CACGTG 1 cut(s) 6647
PspEI GGTNACC 2 cut(s) 3095, 10133
PspFI CCCAGC 2 cut(s) 6358, 10346
PspOMI GGGCCC 1 cut(s) 4118
PspPPI RGGWCCY 2 cut(s) 3689, 5442
PspXI VCTCGAGB 2 cut(s) 49, 480
PsrI GAACNNNNNNTAC 2 cut(s) 1783, 1815
PstI CTGCAG 8 cut(s) 76, 2112, 3378, 3936, 6682, 6694, 7201, 9918
PstNI CAGNNNCTG 4 cut(s) 710, 740, 2237, 8360
PsyI GACNNNGTC 1 cut(s) 440
PvuI CGATCG 1 cut(s) 5450
PvuII CAGCTG 7 cut(s) 1055, 1591, 2519, 3931, 6848, 6880, 7159
RruI TCGCGA 1 cut(s) 6802
RseI CAYNNNNRTG 4 cut(s) 6802, 7479, 9633, 9955
SacI GAGCTC 4 cut(s) 487, 5176, 7634, 7662
SalI GTCGAC 1 cut(s) 1229
SapI GCTCTTC 5 cut(s) 933, 1842, 3374, 3985, 7273
ScaI AGTACT 2 cut(s) 1138, 7095
SfoI GGCGCC 2 cut(s) 120, 4105
Sfr274I CTCGAG 3 cut(s) 49, 480, 4477
SfuI TTCGAA 2 cut(s) 3610, 4145
SlaI CTCGAG 3 cut(s) 49, 480, 4477
SmiMI CAYNNNNRTG 4 cut(s) 6802, 7479, 9633, 9955
SpeI ACTAGT 1 cut(s) 7787
SseBI AGGCCT 2 cut(s) 353, 2054
SspDI GGCGCC 2 cut(s) 118, 4103
SspI AATATT 5 cut(s) 1269, 8092, 8276, 9061, 9328
SstI GAGCTC 4 cut(s) 487, 5176, 7634, 7662
StuI AGGCCT 2 cut(s) 353, 2054
TaqII GACCGA 2 cut(s) 533, 6601
TatI WGTACW 7 cut(s) 848, 1136, 2261, 4094, 4872, 7093, 7816
TauI GCSGC 2 cut(s) 3872, 10083
TspGWI ACGGA 7 cut(s) 4892, 6342, 7462, 9170, 9779, 9928, 10278
Tth111I GACNNNGTC 1 cut(s) 440
Van91I CCANNNNNTGG 3 cut(s) 165, 6632, 7737
Vha464I CTTAAG 4 cut(s) 1360, 1883, 2086, 2570
VneI GTGCAC 1 cut(s) 2478
VspI ATTAAT 4 cut(s) 1004, 3428, 3545, 10044
XagI CCTNNNNNAGG 2 cut(s) 5282, 9917
XceI RCATGY 9 cut(s) 734, 1598, 2268, 4264, 4909, 5038, 7345, 9843, 9900
XcmI CCANNNNNNNNNTGG 2 cut(s) 6413, 7261
XhoI CTCGAG 3 cut(s) 49, 480, 4477
XmiI GTMKAC 3 cut(s) 666, 1230, 8162
XmnI GAANNNNTTC 6 cut(s) 909, 2216, 2296, 2652, 5223, 8064
ZraI GACGTC 1 cut(s) 497
ZrmI AGTACT 2 cut(s) 1138, 7095
Zsp2I ATGCAT 5 cut(s) 2131, 2152, 2714, 4426, 4692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.