Rmu_sc0004759.1_g000015

D-aminoacyl-tRNA deacylase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004759.1
OG0008523
Physical Location & Seq
Forward (+)
53775 .. 56762
2988 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1098 bp
atgggtgagaaaagaaatcaatgggtgagaaaagaaaccgagtttcttaaactgtgttcgaaaaaggctaatgctagccagaacgacgccgtctccactgctcacttttgccactacaaaactggtgtgctgcttccacagtttggcaccaaaaaaatcaccgaacaagatggtgaagctgatcgtggcgacgacgaccgatccggcctccgtcaaccctgccaatgttctcttatccaagcccggctggaggatatgaagagcttcgctaacggagccgttagggttttgctgcacaacaggagcataatcgaagaggacgacttggacgctcgctgggaacaagccaccggcgaacacgtggacgagctcatcttcttgagcaagcacaaagccgcttccagccgccctgcgcttactgttcaccccatcggggttcctcatttgcgaaacggcgacgttttgccgcagggcgggaagccgggctgggccgcgccgccgaatccgaggattgggccctggctcaggctcttgaagagaatcgcgcaggatcgcaacttggtccctgagtttgaggtgactttggaggctacacatcatggaccgattacagacacgcctgccatgttcctggagattggcagtacagatgattgctggaagaggcaggatgcagctgaagttatggctctattagtttgggaaggacttgggcttggagggggagctgctgttggaaactggaacggggggaacgataataaaagagttctgcttgggataggtggtggacattatgtacctcgtcatatggatatagctttgaaagatgatgtctgggtgggccatctaatttcgggttattcgttaccaatggaagatccgagccagtccaagacagaaacaaatacaaaagatatcggtggaacttggaaacaatcaatcaaagtagcatttgaggctacgcaatcggctttccctggtggagaaattgtagctcatcttgatcacaagagtttcaaaagctggcaaaagaatgccataacgagtttcctagaggagcagaacattacaattagcaaacgcgatttcttctga

Protein Analysis

365

Amino Acids

40.41

Weight (kDa)

6.28

Isoelectric Point (pI)

44.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_deacylase PF04414 118 - 359 2.9e-58 D-aminoacyl-tRNA deacylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 146
AccB7I CCANNNNNTGG 1 cut(s) 143
AccII CGCG 3 cut(s) 494, 545, 1086
AciI CCGC 6 cut(s) 396, 406, 467, 474, 492, 497
AclWI GGATC 3 cut(s) 195, 558, 875
AcuI CTGAAG 1 cut(s) 699
AcvI CACGTG 1 cut(s) 361
AcyI GRCGYC 1 cut(s) 87
AfaI GTAC 2 cut(s) 646, 801
AfiI CCNNNNNNNGG 6 cut(s) 143, 250, 433, 473, 512, 525
AflIII ACRYGT 1 cut(s) 358
AgsI TTSAA 3 cut(s) 535, 826, 1021
AjnI CCWGG 3 cut(s) 518, 630, 979
AluBI AGCT 8 cut(s) 179, 264, 370, 677, 728, 821, 998, 1026
AluI AGCT 8 cut(s) 179, 264, 370, 677, 728, 821, 998, 1026
Alw21I GWGCWC 1 cut(s) 372
Alw26I GTCTC 1 cut(s) 97
AlwI GGATC 3 cut(s) 195, 558, 875
AoxI GGCC 4 cut(s) 205, 489, 515, 844
ApaI GGGCCC 1 cut(s) 519
ApeKI GCWGC 4 cut(s) 130, 292, 674, 728
Asp700I GAANNNNTTC 1 cut(s) 263
AspLEI GCGC 3 cut(s) 415, 496, 547
AspS9I GGNCC 6 cut(s) 489, 515, 516, 562, 602, 844
AsuC2I CCSGG 2 cut(s) 244, 483
AsuHPI GGTGA 6 cut(s) 17, 37, 151, 185, 416, 589
AsuII TTCGAA 1 cut(s) 59
AsuNHI GCTAGC 1 cut(s) 74
AvaII GGWCC 2 cut(s) 562, 602
BaeGI GKGCMC 1 cut(s) 519
BanI GGYRCC 1 cut(s) 146
BanII GRGCYC 2 cut(s) 372, 519
BbrPI CACGTG 1 cut(s) 361
Bbv12I GWGCWC 1 cut(s) 372
BbvI GCAGC 4 cut(s) 117, 279, 686, 715
BccI CCATC 3 cut(s) 164, 437, 855
BceAI ACGGC 3 cut(s) 74, 263, 469
BciT130I CCWGG 3 cut(s) 520, 632, 981
BclI TGATCA 1 cut(s) 1006
BcnI CCSGG 2 cut(s) 244, 483
BcoDI GTCTC 1 cut(s) 97
BfaI CTAG 2 cut(s) 75, 1055
BisI GCNGC 9 cut(s) 131, 293, 396, 406, 467, 492, 497, 675, 729
BlsI GCNGC 9 cut(s) 132, 294, 397, 407, 468, 493, 498, 676, 730
Bme1390I CCNGG 5 cut(s) 244, 483, 520, 632, 981
Bme18I GGWCC 2 cut(s) 562, 602
BmgT120I GGNCC 6 cut(s) 489, 515, 516, 562, 602, 844
BmiI GGNNCC 5 cut(s) 148, 277, 438, 517, 564
BmrFI CCNGG 5 cut(s) 244, 483, 520, 632, 981
BmsI GCATC 1 cut(s) 661
BmtI GCTAGC 1 cut(s) 78
BpmI CTGGAG 2 cut(s) 269, 653
Bpu10I CCTNAGC 1 cut(s) 524
Bpu14I TTCGAA 1 cut(s) 59
BpuEI CTTGAG 1 cut(s) 400
BpuMI CCSGG 2 cut(s) 244, 483
BsaAI YACGTR 1 cut(s) 361
BsaHI GRCGYC 1 cut(s) 87
BsaJI CCNNGG 3 cut(s) 506, 518, 979
BsaXI ACNNNNNCTCC 6 cut(s) 77, 107, 717, 747, 978, 1008
Bsc4I CCNNNNNNNGG 6 cut(s) 143, 250, 433, 473, 512, 525
Bse118I RCCGGY 1 cut(s) 350
Bse1I ACTGG 3 cut(s) 127, 746, 889
BseBI CCWGG 3 cut(s) 520, 632, 981
BseDI CCNNGG 3 cut(s) 506, 518, 979
BseGI GGATG 1 cut(s) 676
BseLI CCNNNNNNNGG 6 cut(s) 143, 250, 433, 473, 512, 525
BseMII CTCAG 2 cut(s) 538, 558
BseNI ACTGG 3 cut(s) 127, 746, 889
BseRI GAGGAG 1 cut(s) 1073
BseSI GKGCMC 1 cut(s) 519
BseXI GCAGC 4 cut(s) 117, 279, 686, 715
BseYI CCCAGC 2 cut(s) 336, 486
BsgI GTGCAG 1 cut(s) 278
Bsh1236I CGCG 3 cut(s) 494, 545, 1086
Bsh1285I CGRYCG 1 cut(s) 199
BshFI GGCC 4 cut(s) 207, 491, 517, 846
BshNI GGYRCC 1 cut(s) 146
BsiEI CGRYCG 1 cut(s) 199
BsiHKAI GWGCWC 1 cut(s) 372
BsiSI CCGG 4 cut(s) 204, 244, 351, 482
BslFI GGGAC 1 cut(s) 548
BslI CCNNNNNNNGG 6 cut(s) 143, 250, 433, 473, 512, 525
BsmAI GTCTC 1 cut(s) 97
BsmBI CGTCTC 1 cut(s) 97
BsmFI GGGAC 1 cut(s) 548
BsmI GAATGC 1 cut(s) 1042
BsnI GGCC 4 cut(s) 207, 491, 517, 846
Bsp119I TTCGAA 1 cut(s) 59
Bsp120I GGGCCC 1 cut(s) 515
Bsp1286I GDGCHC 2 cut(s) 372, 519
Bsp143I GATC 5 cut(s) 181, 200, 550, 880, 1006
BspACI CCGC 6 cut(s) 396, 406, 467, 474, 492, 497
BspANI GGCC 4 cut(s) 207, 491, 517, 846
BspCNI CTCAG 2 cut(s) 537, 559
BspFNI CGCG 3 cut(s) 494, 545, 1086
BspLI GGNNCC 5 cut(s) 148, 277, 438, 517, 564
BspOI GCTAGC 1 cut(s) 78
BspPI GGATC 3 cut(s) 195, 558, 875
BspQI GCTCTTC 1 cut(s) 254
BspT104I TTCGAA 1 cut(s) 59
BspT107I GGYRCC 1 cut(s) 146
BsrFI RCCGGY 1 cut(s) 350
BsrI ACTGG 3 cut(s) 127, 746, 889
BssAI RCCGGY 1 cut(s) 350
BssECI CCNNGG 3 cut(s) 506, 518, 979
BssMI GATC 5 cut(s) 181, 200, 550, 880, 1006
BssNI GRCGYC 1 cut(s) 87
Bst2UI CCWGG 3 cut(s) 520, 632, 981
Bst4CI ACNGT 3 cut(s) 54, 141, 421
Bst6I CTCTTC 4 cut(s) 254, 309, 530, 656
BstACI GRCGYC 1 cut(s) 87
BstBAI YACGTR 1 cut(s) 361
BstBI TTCGAA 1 cut(s) 59
BstC8I GCNNGC 5 cut(s) 76, 334, 386, 621, 1028
BstDEI CTNAG 2 cut(s) 524, 567
BstENI CCTNNNNNAGG 1 cut(s) 523
BstF5I GGATG 1 cut(s) 676
BstFNI CGCG 3 cut(s) 494, 545, 1086
BstHHI GCGC 3 cut(s) 415, 496, 547
BstKTI GATC 5 cut(s) 184, 203, 553, 883, 1009
BstMAI GTCTC 1 cut(s) 97
BstMBI GATC 5 cut(s) 181, 200, 550, 880, 1006
BstMCI CGRYCG 1 cut(s) 199
BstMWI GCNNNNNNNGC 2 cut(s) 275, 959
BstNI CCWGG 3 cut(s) 520, 632, 981
BstSCI CCNGG 5 cut(s) 242, 481, 518, 630, 979
BstSLI GKGCMC 1 cut(s) 519
BstUI CGCG 3 cut(s) 494, 545, 1086
BstV1I GCAGC 4 cut(s) 117, 279, 686, 715
BstX2I RGATCY 1 cut(s) 880
BstXI CCANNNNNNTGG 1 cut(s) 631
BstYI RGATCY 1 cut(s) 880
BsuRI GGCC 4 cut(s) 207, 491, 517, 846
BtsCI GGATG 1 cut(s) 676
BtsI GCAGTG 1 cut(s) 96
BtsIMutI CAGTG 1 cut(s) 96
Cac8I GCNNGC 5 cut(s) 76, 334, 386, 621, 1028
CfoI GCGC 3 cut(s) 415, 496, 547
Cfr10I RCCGGY 1 cut(s) 350
Cfr13I GGNCC 6 cut(s) 489, 515, 516, 562, 602, 844
CseI GACGC 2 cut(s) 95, 338
Csp6I GTAC 2 cut(s) 645, 800
CviAII CATG 2 cut(s) 599, 625
CviQI GTAC 2 cut(s) 645, 800
DdeI CTNAG 2 cut(s) 524, 567
DpnI GATC 5 cut(s) 183, 202, 552, 882, 1008
DpnII GATC 5 cut(s) 181, 200, 550, 880, 1006
Eam1104I CTCTTC 4 cut(s) 254, 309, 530, 656
EarI CTCTTC 4 cut(s) 254, 309, 530, 656
Ecl136II GAGCTC 1 cut(s) 370
Eco24I GRGCYC 2 cut(s) 372, 519
Eco32I GATATC 1 cut(s) 919
Eco47I GGWCC 2 cut(s) 562, 602
Eco53kI GAGCTC 1 cut(s) 370
Eco57I CTGAAG 1 cut(s) 699
Eco72I CACGTG 1 cut(s) 361
EcoICRI GAGCTC 1 cut(s) 370
EcoNI CCTNNNNNAGG 1 cut(s) 523
EcoO109I RGGNCCY 1 cut(s) 516
EcoRII CCWGG 3 cut(s) 518, 630, 979
EcoRV GATATC 1 cut(s) 919
EcoT38I GRGCYC 2 cut(s) 372, 519
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 2 cut(s) 602, 628
FaqI GGGAC 1 cut(s) 548
FatI CATG 2 cut(s) 598, 624
FauI CCCGC 1 cut(s) 467
FauNDI CATATG 1 cut(s) 810
FbaI TGATCA 1 cut(s) 1006
Fnu4HI GCNGC 9 cut(s) 131, 293, 396, 406, 467, 492, 497, 675, 729
FokI GGATG 1 cut(s) 683
FriOI GRGCYC 2 cut(s) 372, 519
Fsp4HI GCNGC 9 cut(s) 131, 293, 396, 406, 467, 492, 497, 675, 729
FspBI CTAG 2 cut(s) 75, 1055
GlaI GCGC 3 cut(s) 414, 495, 546
GluI GCNGC 9 cut(s) 131, 293, 396, 406, 467, 492, 497, 675, 729
GsaI CCCAGC 2 cut(s) 340, 490
GsuI CTGGAG 2 cut(s) 269, 653
HaeIII GGCC 4 cut(s) 207, 491, 517, 846
HapII CCGG 4 cut(s) 204, 244, 351, 482
HgaI GACGC 2 cut(s) 95, 338
HhaI GCGC 3 cut(s) 415, 496, 547
Hin1I GRCGYC 1 cut(s) 87
Hin1II CATG 2 cut(s) 602, 628
Hin6I GCGC 3 cut(s) 413, 494, 545
HinP1I GCGC 3 cut(s) 413, 494, 545
HincII GTYRAC 1 cut(s) 215
HindII GTYRAC 1 cut(s) 215
HinfI GANTC 2 cut(s) 502, 540
HpaII CCGG 4 cut(s) 204, 244, 351, 482
HphI GGTGA 6 cut(s) 17, 37, 151, 185, 416, 589
Hpy166II GTNNAC 4 cut(s) 215, 364, 424, 791
Hpy188I TCNGA 3 cut(s) 507, 885, 1097
Hpy188III TCNNGA 3 cut(s) 379, 532, 1004
Hpy8I GTNNAC 4 cut(s) 215, 364, 424, 791
Hpy99I CGWCG 4 cut(s) 89, 194, 197, 461
HpyAV CCTTC 1 cut(s) 698
HpyCH4III ACNGT 3 cut(s) 54, 141, 421
HpyCH4IV ACGT 2 cut(s) 360, 459
HpyCH4V TGCA 2 cut(s) 295, 674
HpyF10VI GCNNNNNNNGC 2 cut(s) 275, 959
HpyF3I CTNAG 2 cut(s) 524, 567
HpySE526I ACGT 2 cut(s) 360, 459
Hsp92I GRCGYC 1 cut(s) 87
Hsp92II CATG 2 cut(s) 602, 628
HspAI GCGC 3 cut(s) 413, 494, 545
Ksp22I TGATCA 1 cut(s) 1006
Kzo9I GATC 5 cut(s) 181, 200, 550, 880, 1006
LguI GCTCTTC 1 cut(s) 254
LmnI GCTCC 4 cut(s) 275, 303, 725, 1060
Lsp1109I GCAGC 4 cut(s) 117, 279, 686, 715
LweI GCATC 1 cut(s) 661
MaeI CTAG 2 cut(s) 75, 1055
MaeII ACGT 2 cut(s) 360, 459
MaeIII GTNAC 2 cut(s) 577, 867
MalI GATC 5 cut(s) 183, 202, 552, 882, 1008
MboI GATC 5 cut(s) 181, 200, 550, 880, 1006
MboII GAAGA 7 cut(s) 271, 326, 367, 547, 673, 890, 1084
MflI RGATCY 1 cut(s) 880
MhlI GDGCHC 2 cut(s) 372, 519
MluCI AATT 3 cut(s) 852, 990, 1074
MmeI TCCRAC 1 cut(s) 715
MroXI GAANNNNTTC 1 cut(s) 263
MseI TTAA 1 cut(s) 48
MspA1I CMGCKG 1 cut(s) 677
MspI CCGG 4 cut(s) 204, 244, 351, 482
MspR9I CCNGG 5 cut(s) 244, 483, 520, 632, 981
Mva1269I GAATGC 1 cut(s) 1042
MvaI CCWGG 3 cut(s) 520, 632, 981
MvnI CGCG 3 cut(s) 494, 545, 1086
MwoI GCNNNNNNNGC 2 cut(s) 275, 959
NciI CCSGG 2 cut(s) 244, 483
NdeI CATATG 1 cut(s) 810
NdeII GATC 5 cut(s) 181, 200, 550, 880, 1006
NheI GCTAGC 1 cut(s) 74
NlaIII CATG 2 cut(s) 602, 628
NlaIV GGNNCC 5 cut(s) 148, 277, 438, 517, 564
NmuCI GTSAC 1 cut(s) 577
NspV TTCGAA 1 cut(s) 59
PciSI GCTCTTC 1 cut(s) 254
PcsI WCGNNNNNNNCGW 3 cut(s) 318, 753, 863
PctI GAATGC 1 cut(s) 1042
PdmI GAANNNNTTC 1 cut(s) 263
PfeI GAWTC 2 cut(s) 502, 540
PflFI GACNNNGTC 1 cut(s) 89
PflMI CCANNNNNTGG 1 cut(s) 143
PfoI TCCNGGA 1 cut(s) 630
PkrI GCNGC 9 cut(s) 132, 294, 397, 407, 468, 493, 498, 676, 730
PmaCI CACGTG 1 cut(s) 361
PmlI CACGTG 1 cut(s) 361
Ppu21I YACGTR 1 cut(s) 361
Psp124BI GAGCTC 1 cut(s) 372
Psp6I CCWGG 3 cut(s) 518, 630, 979
PspCI CACGTG 1 cut(s) 361
PspFI CCCAGC 2 cut(s) 336, 486
PspGI CCWGG 3 cut(s) 518, 630, 979
PspN4I GGNNCC 5 cut(s) 148, 277, 438, 517, 564
PspOMI GGGCCC 1 cut(s) 515
PspPI GGNCC 6 cut(s) 489, 515, 516, 562, 602, 844
PsuI RGATCY 1 cut(s) 880
PsyI GACNNNGTC 1 cut(s) 89
PvuII CAGCTG 1 cut(s) 677
RsaI GTAC 2 cut(s) 646, 801
RsaNI GTAC 2 cut(s) 645, 800
SacI GAGCTC 1 cut(s) 372
SapI GCTCTTC 1 cut(s) 254
SaqAI TTAA 1 cut(s) 48
SatI GCNGC 9 cut(s) 131, 293, 396, 406, 467, 492, 497, 675, 729
Sau3AI GATC 5 cut(s) 181, 200, 550, 880, 1006
Sau96I GGNCC 6 cut(s) 489, 515, 516, 562, 602, 844
ScrFI CCNGG 5 cut(s) 244, 483, 520, 632, 981
SduI GDGCHC 2 cut(s) 372, 519
SfaNI GCATC 1 cut(s) 661
SfuI TTCGAA 1 cut(s) 59
SgrAI CRCCGGYG 1 cut(s) 350
SinI GGWCC 2 cut(s) 562, 602
SmlI CTYRAG 1 cut(s) 379
SmoI CTYRAG 1 cut(s) 379
Sse9I AATT 3 cut(s) 852, 990, 1074
SsiI CCGC 6 cut(s) 396, 406, 467, 474, 492, 497
SspMI CTAG 2 cut(s) 75, 1055
SstI GAGCTC 1 cut(s) 372
StyD4I CCNGG 5 cut(s) 242, 481, 518, 630, 979
TaaI ACNGT 3 cut(s) 54, 141, 421
TaiI ACGT 2 cut(s) 363, 462
TaqI TCGA 2 cut(s) 59, 312
TaqII GACCGA 2 cut(s) 213, 619
TasI AATT 3 cut(s) 852, 990, 1074
TatI WGTACW 1 cut(s) 644
TauI GCSGC 5 cut(s) 398, 408, 469, 494, 499
TfiI GAWTC 2 cut(s) 502, 540
Tru1I TTAA 1 cut(s) 48
Tru9I TTAA 1 cut(s) 48
TscAI CASTG 1 cut(s) 103
TseFI GTSAC 1 cut(s) 577
TseI GCWGC 4 cut(s) 130, 292, 674, 728
Tsp45I GTSAC 1 cut(s) 577
TspDTI ATGAA 1 cut(s) 272
TspGWI ACGGA 2 cut(s) 200, 288
TspRI CASTG 1 cut(s) 103
Tth111I GACNNNGTC 1 cut(s) 89
Van91I CCANNNNNTGG 1 cut(s) 143
VpaK11BI GGWCC 2 cut(s) 562, 602
XagI CCTNNNNNAGG 1 cut(s) 523
XcmI CCANNNNNNNNNTGG 1 cut(s) 119
XmnI GAANNNNTTC 1 cut(s) 263
XspI CTAG 2 cut(s) 75, 1055
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.