Rmu_sc0004760.1_g000012

Auxin-induced protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004760.1
OG0006280
Physical Location & Seq
Forward (+)
64749 .. 66152
1404 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 714 bp
atggaagtattgaagccaaggacaacctcaggtttggctaagcttgtaggcatattattttgcacggcaggggcggcgacccttgccgtatacaaaggtccccatttcaatctcctggggcatcataatctcttacatcatatcgaagaacatgaaggccatgttccttctggcaaaacatggataaagggttgcttcctcatgctgatgtccaacaccttttggggcttgtggcttgttttgcaggcacgagttatgaaaagctacccctcaaagcttctctttactactctacaatgctttctaagttcaatccagacgtttgtcattgccatcgctgttgagagaaacccctatgaatggaagctcggatggaatctcaaacttgttgccatagcttactgcggaattgtggtgaccggcattacttaccatttacaagcatgggtaatggagaagaaggacccggttttcttggctatgtccacaccattggctttggtcatcacactctttgcttctgcaattctcctgggagaaatcattagtctgggaagtatcttgggtgggctcttattggttggagggctttatagtgttttatgggcgaagagcagagagcagagtatagttgatgaaagttgcttaagagctgaagttgtcaaagactcctcaaaactgaaagaaacagaggacttcgtttctgtagcgtaa

Protein Analysis

237

Amino Acids

26.16

Weight (kDa)

8.55

Isoelectric Point (pI)

35.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 63 - 201 7.7e-15 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 90
AciI CCGC 2 cut(s) 74, 405
AcuI CTGAAG 1 cut(s) 675
AfiI CCNNNNNNNGG 1 cut(s) 360
AflII CTTAAG 1 cut(s) 646
AgsI TTSAA 3 cut(s) 13, 109, 312
AjnI CCWGG 2 cut(s) 114, 531
AluBI AGCT 6 cut(s) 43, 264, 277, 367, 398, 653
AluI AGCT 6 cut(s) 43, 264, 277, 367, 398, 653
AoxI GGCC 1 cut(s) 157
AspS9I GGNCC 2 cut(s) 98, 463
AsuC2I CCSGG 1 cut(s) 467
AsuHPI GGTGA 1 cut(s) 427
AvaII GGWCC 2 cut(s) 98, 463
AxyI CCTNAGG 1 cut(s) 28
BanII GRGCYC 1 cut(s) 573
BauI CACGAG 1 cut(s) 249
BccI CCATC 2 cut(s) 341, 366
BceAI ACGGC 2 cut(s) 71, 81
BciT130I CCWGG 2 cut(s) 116, 533
BcnI CCSGG 1 cut(s) 467
BfmI CTRYAG 1 cut(s) 705
BfrI CTTAAG 1 cut(s) 646
BisI GCNGC 1 cut(s) 75
BlpI GCTNAGC 1 cut(s) 39
BlsI GCNGC 1 cut(s) 76
Bme1390I CCNGG 3 cut(s) 116, 467, 533
Bme18I GGWCC 2 cut(s) 98, 463
BmgT120I GGNCC 2 cut(s) 98, 463
BmiI GGNNCC 2 cut(s) 100, 465
BmrFI CCNGG 3 cut(s) 116, 467, 533
BmsI GCATC 1 cut(s) 130
BoxI GACNNNNGTC 1 cut(s) 323
Bpu1102I GCTNAGC 1 cut(s) 39
BpuMI CCSGG 1 cut(s) 467
BsaJI CCNNGG 3 cut(s) 17, 115, 532
Bsc4I CCNNNNNNNGG 1 cut(s) 360
Bse118I RCCGGY 1 cut(s) 419
Bse21I CCTNAGG 1 cut(s) 28
Bse3DI GCAATG 1 cut(s) 327
BseBI CCWGG 2 cut(s) 116, 533
BseDI CCNNGG 3 cut(s) 17, 115, 532
BseGI GGATG 1 cut(s) 377
BseLI CCNNNNNNNGG 1 cut(s) 360
BseMI GCAATG 1 cut(s) 327
BseMII CTCAG 1 cut(s) 42
BseRI GAGGAG 1 cut(s) 661
BshFI GGCC 1 cut(s) 159
BsiSI CCGG 2 cut(s) 420, 467
BslFI GGGAC 1 cut(s) 84
BslI CCNNNNNNNGG 1 cut(s) 360
BsmFI GGGAC 1 cut(s) 84
BsnI GGCC 1 cut(s) 159
Bsp1286I GDGCHC 1 cut(s) 573
Bsp1720I GCTNAGC 1 cut(s) 39
BspACI CCGC 2 cut(s) 74, 405
BspANI GGCC 1 cut(s) 159
BspCNI CTCAG 1 cut(s) 41
BspLI GGNNCC 2 cut(s) 100, 465
BspQI GCTCTTC 1 cut(s) 605
BspTI CTTAAG 1 cut(s) 646
BsrDI GCAATG 1 cut(s) 327
BsrFI RCCGGY 1 cut(s) 419
BssAI RCCGGY 1 cut(s) 419
BssECI CCNNGG 3 cut(s) 17, 115, 532
BssNAI GTATAC 1 cut(s) 91
BssSI CACGAG 1 cut(s) 249
BssT1I CCWWGG 1 cut(s) 17
Bst1107I GTATAC 1 cut(s) 91
Bst2BI CACGAG 1 cut(s) 249
Bst2UI CCWGG 2 cut(s) 116, 533
Bst6I CTCTTC 1 cut(s) 605
BstAFI CTTAAG 1 cut(s) 646
BstC8I GCNNGC 1 cut(s) 246
BstDEI CTNAG 3 cut(s) 28, 39, 305
BstEII GGTNACC 1 cut(s) 415
BstF5I GGATG 1 cut(s) 377
BstMWI GCNNNNNNNGC 3 cut(s) 74, 83, 241
BstNI CCWGG 2 cut(s) 116, 533
BstPAI GACNNNNGTC 1 cut(s) 323
BstPI GGTNACC 1 cut(s) 415
BstSCI CCNGG 3 cut(s) 114, 465, 531
BstSFI CTRYAG 1 cut(s) 705
BstXI CCANNNNNNTGG 1 cut(s) 493
BstZ17I GTATAC 1 cut(s) 91
Bsu36I CCTNAGG 1 cut(s) 28
BsuRI GGCC 1 cut(s) 159
BtgZI GCGATG 1 cut(s) 319
BtsCI GGATG 1 cut(s) 377
Cac8I GCNNGC 1 cut(s) 246
Cfr10I RCCGGY 1 cut(s) 419
Cfr13I GGNCC 2 cut(s) 98, 463
CviAII CATG 5 cut(s) 152, 161, 180, 202, 444
DdeI CTNAG 3 cut(s) 28, 39, 305
Eam1104I CTCTTC 1 cut(s) 605
EarI CTCTTC 1 cut(s) 605
Eco130I CCWWGG 1 cut(s) 17
Eco24I GRGCYC 1 cut(s) 573
Eco47I GGWCC 2 cut(s) 98, 463
Eco57I CTGAAG 1 cut(s) 675
Eco81I CCTNAGG 1 cut(s) 28
Eco91I GGTNACC 1 cut(s) 415
EcoO109I RGGNCCY 2 cut(s) 98, 463
EcoO65I GGTNACC 1 cut(s) 415
EcoRII CCWGG 2 cut(s) 114, 531
EcoT14I CCWWGG 1 cut(s) 17
EcoT38I GRGCYC 1 cut(s) 573
ErhI CCWWGG 1 cut(s) 17
FaeI CATG 5 cut(s) 155, 164, 183, 205, 447
FalI AAGNNNNNCTT 4 cut(s) 179, 211, 266, 298
FaqI GGGAC 1 cut(s) 84
FatI CATG 5 cut(s) 151, 160, 179, 201, 443
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 1 cut(s) 75
FokI GGATG 1 cut(s) 384
FriOI GRGCYC 1 cut(s) 573
Fsp4HI GCNGC 1 cut(s) 75
GluI GCNGC 1 cut(s) 75
HaeIII GGCC 1 cut(s) 159
HapII CCGG 2 cut(s) 420, 467
Hin1II CATG 5 cut(s) 155, 164, 183, 205, 447
HindIII AAGCTT 2 cut(s) 41, 275
HinfI GANTC 2 cut(s) 376, 668
HpaII CCGG 2 cut(s) 420, 467
HphI GGTGA 1 cut(s) 427
Hpy166II GTNNAC 2 cut(s) 91, 486
Hpy188I TCNGA 1 cut(s) 371
Hpy188III TCNNGA 1 cut(s) 316
Hpy8I GTNNAC 2 cut(s) 91, 486
HpyAV CCTTC 3 cut(s) 149, 177, 454
HpyCH4IV ACGT 1 cut(s) 320
HpyCH4V TGCA 3 cut(s) 63, 244, 524
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 83, 241
HpyF3I CTNAG 3 cut(s) 28, 39, 305
HpySE526I ACGT 1 cut(s) 320
Hsp92II CATG 5 cut(s) 155, 164, 183, 205, 447
LguI GCTCTTC 1 cut(s) 605
LweI GCATC 1 cut(s) 130
MaeII ACGT 1 cut(s) 320
MaeIII GTNAC 1 cut(s) 415
MboII GAAGA 3 cut(s) 158, 469, 622
MhlI GDGCHC 1 cut(s) 573
MluCI AATT 2 cut(s) 408, 525
MlyI GAGTC 1 cut(s) 662
MmeI TCCRAC 2 cut(s) 237, 562
MnlI CCTC 6 cut(s) 37, 209, 280, 578, 682, 685
MseI TTAA 1 cut(s) 647
MslI CAYNNNNRTG 1 cut(s) 206
MspCI CTTAAG 1 cut(s) 646
MspI CCGG 2 cut(s) 420, 467
MspR9I CCNGG 3 cut(s) 116, 467, 533
MvaI CCWGG 2 cut(s) 116, 533
MwoI GCNNNNNNNGC 3 cut(s) 74, 83, 241
NciI CCSGG 1 cut(s) 467
NlaIII CATG 5 cut(s) 155, 164, 183, 205, 447
NlaIV GGNNCC 2 cut(s) 100, 465
NmuCI GTSAC 1 cut(s) 415
PciSI GCTCTTC 1 cut(s) 605
PfeI GAWTC 1 cut(s) 376
PkrI GCNGC 1 cut(s) 76
PleI GAGTC 1 cut(s) 662
PpsI GAGTC 1 cut(s) 662
PpuMI RGGWCCY 2 cut(s) 98, 463
PshAI GACNNNNGTC 1 cut(s) 323
Psp5II RGGWCCY 2 cut(s) 98, 463
Psp6I CCWGG 2 cut(s) 114, 531
PspEI GGTNACC 1 cut(s) 415
PspGI CCWGG 2 cut(s) 114, 531
PspN4I GGNNCC 2 cut(s) 100, 465
PspPI GGNCC 2 cut(s) 98, 463
PspPPI RGGWCCY 2 cut(s) 98, 463
RseI CAYNNNNRTG 1 cut(s) 206
SapI GCTCTTC 1 cut(s) 605
SaqAI TTAA 1 cut(s) 647
SatI GCNGC 1 cut(s) 75
Sau96I GGNCC 2 cut(s) 98, 463
SchI GAGTC 1 cut(s) 662
ScrFI CCNGG 3 cut(s) 116, 467, 533
SduI GDGCHC 1 cut(s) 573
SfaNI GCATC 1 cut(s) 130
SfcI CTRYAG 1 cut(s) 705
SinI GGWCC 2 cut(s) 98, 463
SmiMI CAYNNNNRTG 1 cut(s) 206
SmlI CTYRAG 1 cut(s) 646
SmoI CTYRAG 1 cut(s) 646
Sse9I AATT 2 cut(s) 408, 525
SsiI CCGC 2 cut(s) 74, 405
StyD4I CCNGG 3 cut(s) 114, 465, 531
StyI CCWWGG 1 cut(s) 17
TaiI ACGT 1 cut(s) 323
TaqI TCGA 1 cut(s) 144
TasI AATT 2 cut(s) 408, 525
TauI GCSGC 1 cut(s) 77
TfiI GAWTC 1 cut(s) 376
Tru1I TTAA 1 cut(s) 647
Tru9I TTAA 1 cut(s) 647
TseFI GTSAC 1 cut(s) 415
Tsp45I GTSAC 1 cut(s) 415
TspDTI ATGAA 4 cut(s) 168, 272, 372, 651
Vha464I CTTAAG 1 cut(s) 646
VpaK11BI GGWCC 2 cut(s) 98, 463
XcmI CCANNNNNNNNNTGG 1 cut(s) 167
XmiI GTMKAC 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.