Rmu_sc0006378.1_g000003
ERF Family

Radical SAM superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006378.1
OG0007961
Physical Location & Seq
Reverse (-)
6651 .. 12231
5581 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2313 bp
atggaagggttgcttccccttctataccatgccatcaagaagcgaagatcaaaacacaagcacagatgcttttcggagagcaattcaaaaagtagctaccgtctcctcggttgtggccatgacctaaccttggaaggcttgtctcattgtcaaaaaggatccgagttccaacctctggtcactgctgaattattagagcagcgatctgcacttgactttaatcattcgtgtagctacgaggaagaagatggtgtctctgcgggggttccttttgtgccaagttcctcaaaagataggatttcacagcgaagtcataactcatccgcctccgtcaccgcaacccccaaaacgcccaaaatcatgcaaagtgctcgcaaaacgacgccgttccggtcaattttcgacgccggagctctgaaatcagagctggagaactccgggatcaagcccagcttcatgccctacatatggaagcaggtgatccaaaacccctccgcccaactcgaccaaatcccctccttgccctccgccgcataccctcttctcaactccaaattcaaaaccctaacctcctccgtccactccgccaccgactcctccgaccacctcaccaccaagctcctcatcaagctccaggtttgttttccgcacaccacctgttcgatgaaatgtctgaatgacatatggaagcaggtgatccaaaacccctccgcccaactcgaccaaatcccctccttgccctccgccgcataccctcttctaaactccaaattcaaaaccctaacctcctccgtccactccgccaccgactcctccgaccacctcaccaccaagctcctcatcaagctccagaacggcgctctggtggaggccgtgatcatgaggtacgatagcagcttggggaagcacaatggcaagtcccaccacggcggcgcaaggtccaccttgtgcgtatcgtctcaggttggttgtaaaatgggttgcacgttctgtgccactgggagtatggggtttaagagcaatttgacctctggggagattgtggagcagttggttcatgcttctcgcatttcgcctatacgcaatgttgttttcatgggaatgggggaaccgttgaataactataatgccttagtggaagcaattcatgtcatgactggattaccatttcagctgtcgccaaagagaatcacagtgtcaacggttggtattattcatgctatcaacaagttccataatgatctccctggactgaatttagcagtttcactgcatgcacctgtccaagaaattcgttgtcagataatgcctgcagctcgggcctttcccttagtaaaacttatggattcactgcaacaatatcaaaagaacagtcagcagaaaattttcattgaatacataatgcttgacggagtgaacgatgaagaagaacatgcgcaccagcttgggaaattgctagatacatttcaagtggtaattaatttaataccttttaacccaattggtagtctgagcaaattcaaaaccactgccgaagaaaaagtatctgtttttcagaagattctaagaggtacgtatggcatccgaacaaccgttcgcaaggaaatgggtcaggacataagtggagcgtgtggtcagttggtggtgaacatacccaataagaggtcaagtgaccctctaactgatatagaagatcttcagggaatgggggaaccgttgaataactataatgccttagtggaagcaattcatgtcatgactggattaccatttcagctgtcgccaaagagaatcacagtgtcaacggttggtattattcatgctatcaacaagttccataatgatctccctggactgaatttagcagtttcactgcatgcacctgtccaagaaattcgttgtcagataatgcctgcagctcgggcctttcccttagtaaaacttatggattcactgcaacaatatcaaaagaacagtcagcagaaaattttcattgaatacataatgcttgacggagtgaacgatgaagaagaacatgcgcaccagcttgggaaattgctagatacatttcaagtggtaattaatttaataccttttaacccaattggtagtctgagcaaattcaaaaccactgccgaagaaaaagtatctgtttttcagaagattctaagaggtacgtatggcatccgaacaaccgttcgcaaggaaatgggtcaggacataagtggagcgtgtggtcagttggtggtgaacatacccaataagaggtcaagtgaccctctaactgatatagaagatcttcaggtcagatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 Radical_SAM

Protein Analysis

770

Amino Acids

84.98

Weight (kDa)

8.87

Isoelectric Point (pI)

47.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 319 - 479 1.4e-10 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 466, 682
Acc16I TGCGCA 2 cut(s) 1437, 2040
Acc36I ACCTGC 2 cut(s) 466, 682
AccB7I CCANNNNNTGG 1 cut(s) 175
AclWI GGATC 5 cut(s) 153, 166, 449, 475, 691
AcoI YGGCCR 1 cut(s) 115
AcuI CTGAAG 2 cut(s) 1682, 2285
AcyI GRCGYC 2 cut(s) 383, 405
AdeI CACNNNGTG 1 cut(s) 948
AfaI GTAC 3 cut(s) 887, 1573, 2176
AfiI CCNNNNNNNGG 5 cut(s) 130, 175, 468, 1662, 2265
AjnI CCWGG 3 cut(s) 633, 1246, 1849
Alw21I GWGCWC 2 cut(s) 373, 415
Alw26I GTCTC 4 cut(s) 107, 147, 259, 963
AlwI GGATC 5 cut(s) 153, 166, 449, 475, 691
Ama87I CYCGRG 2 cut(s) 1317, 1920
AoxI GGCC 4 cut(s) 115, 870, 1320, 1923
ApeKI GCWGC 4 cut(s) 199, 894, 1313, 1916
ArsI GACNNNNNNTTYG 4 cut(s) 1357, 1389, 1960, 1992
AseI ATTAAT 2 cut(s) 1479, 2082
Asp700I GAANNNNTTC 6 cut(s) 1143, 1385, 1695, 1746, 1988, 2298
AspLEI GCGC 4 cut(s) 860, 935, 1438, 2041
AspS9I GGNCC 3 cut(s) 939, 1320, 1923
AsuC2I CCSGG 1 cut(s) 439
AsuHPI GGTGA 7 cut(s) 325, 490, 601, 706, 817, 1657, 2260
AvaI CYCGRG 2 cut(s) 1317, 1920
AvaII GGWCC 1 cut(s) 939
BalI TGGCCA 1 cut(s) 117
BamHI GGATCC 1 cut(s) 158
BanII GRGCYC 1 cut(s) 415
Bbv12I GWGCWC 2 cut(s) 373, 415
BbvI GCAGC 4 cut(s) 211, 906, 1325, 1928
BccI CCATC 2 cut(s) 41, 242
BceAI ACGGC 4 cut(s) 370, 857, 871, 943
BcgI CGANNNNNNTGC 2 cut(s) 353, 387
BciT130I CCWGG 3 cut(s) 635, 1248, 1851
BclI TGATCA 1 cut(s) 876
BcnI CCSGG 1 cut(s) 439
BcoDI GTCTC 4 cut(s) 107, 147, 259, 963
BfaI CTAG 2 cut(s) 1457, 2060
BfmI CTRYAG 2 cut(s) 1311, 1914
BfoI RGCGCY 1 cut(s) 861
BfuAI ACCTGC 2 cut(s) 466, 682
BglII AGATCT 2 cut(s) 1693, 2296
BisI GCNGC 7 cut(s) 200, 531, 747, 895, 931, 1314, 1917
BlsI GCNGC 7 cut(s) 201, 532, 748, 896, 932, 1315, 1918
Bme1390I CCNGG 4 cut(s) 439, 635, 1248, 1851
Bme18I GGWCC 1 cut(s) 939
BmeT110I CYCGRG 2 cut(s) 1317, 1920
BmgT120I GGNCC 3 cut(s) 939, 1320, 1923
BmiI GGNNCC 4 cut(s) 160, 267, 1110, 1713
BmrFI CCNGG 4 cut(s) 439, 635, 1248, 1851
BmrI ACTGGG 1 cut(s) 1008
BmsI GCATC 3 cut(s) 56, 1590, 2193
BmuI ACTGGG 1 cut(s) 1008
BpmI CTGGAG 3 cut(s) 449, 617, 833
BpuMI CCSGG 1 cut(s) 439
BsaAI YACGTR 2 cut(s) 1575, 2178
BsaHI GRCGYC 2 cut(s) 383, 405
BsaJI CCNNGG 5 cut(s) 106, 129, 925, 1246, 1849
BsaWI WCCGGW 1 cut(s) 390
Bsc4I CCNNNNNNNGG 5 cut(s) 130, 175, 468, 1662, 2265
Bse1I ACTGG 3 cut(s) 1003, 1162, 1765
Bse3DI GCAATG 1 cut(s) 1090
BseBI CCWGG 3 cut(s) 635, 1248, 1851
BseDI CCNNGG 5 cut(s) 106, 129, 925, 1246, 1849
BseGI GGATG 3 cut(s) 320, 1581, 2184
BseLI CCNNNNNNNGG 5 cut(s) 130, 175, 468, 1662, 2265
BseMI GCAATG 1 cut(s) 1090
BseMII CTCAG 3 cut(s) 974, 1502, 2105
BseNI ACTGG 3 cut(s) 1003, 1162, 1765
BseRI GAGGAG 7 cut(s) 95, 562, 586, 611, 778, 802, 827
BseXI GCAGC 4 cut(s) 211, 906, 1325, 1928
BseYI CCCAGC 1 cut(s) 449
BsgI GTGCAG 1 cut(s) 192
BshFI GGCC 4 cut(s) 117, 872, 1322, 1925
BsiHKAI GWGCWC 2 cut(s) 373, 415
BsiHKCI CYCGRG 2 cut(s) 1317, 1920
BsiSI CCGG 3 cut(s) 391, 408, 438
BslFI GGGAC 1 cut(s) 904
BslI CCNNNNNNNGG 5 cut(s) 130, 175, 468, 1662, 2265
BsmAI GTCTC 4 cut(s) 107, 147, 259, 963
BsmBI CGTCTC 2 cut(s) 107, 963
BsmFI GGGAC 1 cut(s) 904
BsnI GGCC 4 cut(s) 117, 872, 1322, 1925
BsoBI CYCGRG 2 cut(s) 1317, 1920
Bsp1286I GDGCHC 2 cut(s) 373, 415
BspANI GGCC 4 cut(s) 117, 872, 1322, 1925
BspCNI CTCAG 3 cut(s) 973, 1503, 2106
BspHI TCATGA 3 cut(s) 879, 1152, 1755
BspLI GGNNCC 4 cut(s) 160, 267, 1110, 1713
BspMAI CTGCAG 2 cut(s) 1315, 1918
BspMI ACCTGC 2 cut(s) 466, 682
BspPI GGATC 5 cut(s) 153, 166, 449, 475, 691
BsrDI GCAATG 1 cut(s) 1090
BsrI ACTGG 3 cut(s) 1003, 1162, 1765
BssECI CCNNGG 5 cut(s) 106, 129, 925, 1246, 1849
BssNI GRCGYC 2 cut(s) 383, 405
BssT1I CCWWGG 1 cut(s) 129
Bst2UI CCWGG 3 cut(s) 635, 1248, 1851
Bst6I CTCTTC 2 cut(s) 546, 762
BstACI GRCGYC 2 cut(s) 383, 405
BstBAI YACGTR 2 cut(s) 1575, 2178
BstC8I GCNNGC 5 cut(s) 373, 1275, 1311, 1878, 1914
BstDEI CTNAG 9 cut(s) 960, 1132, 1330, 1511, 1565, 1735, 1933, 2114, 2168
BstDSI CCRYGG 1 cut(s) 925
BstF5I GGATG 3 cut(s) 320, 1581, 2184
BstH2I RGCGCY 1 cut(s) 861
BstHHI GCGC 4 cut(s) 860, 935, 1438, 2041
BstMAI GTCTC 4 cut(s) 107, 147, 259, 963
BstMWI GCNNNNNNNGC 2 cut(s) 1319, 1922
BstNI CCWGG 3 cut(s) 635, 1248, 1851
BstNSI RCATGY 4 cut(s) 1277, 1436, 1880, 2039
BstSCI CCNGG 4 cut(s) 437, 633, 1246, 1849
BstSFI CTRYAG 2 cut(s) 1311, 1914
BstSNI TACGTA 2 cut(s) 1575, 2178
BstV1I GCAGC 4 cut(s) 211, 906, 1325, 1928
BstX2I RGATCY 3 cut(s) 158, 1693, 2296
BstYI RGATCY 3 cut(s) 158, 1693, 2296
BsuRI GGCC 4 cut(s) 117, 872, 1322, 1925
BtgI CCRYGG 1 cut(s) 925
BtsCI GGATG 3 cut(s) 320, 1581, 2184
BtsI GCAGTG 7 cut(s) 180, 1268, 1349, 1527, 1871, 1952, 2130
BveI ACCTGC 2 cut(s) 466, 682
Cac8I GCNNGC 5 cut(s) 373, 1275, 1311, 1878, 1914
CciI TCATGA 3 cut(s) 879, 1152, 1755
CfoI GCGC 4 cut(s) 860, 935, 1438, 2041
Cfr13I GGNCC 3 cut(s) 939, 1320, 1923
CseI GACGC 2 cut(s) 391, 413
Csp6I GTAC 3 cut(s) 886, 1572, 2175
CviQI GTAC 3 cut(s) 886, 1572, 2175
DdeI CTNAG 9 cut(s) 960, 1132, 1330, 1511, 1565, 1735, 1933, 2114, 2168
DraIII CACNNNGTG 1 cut(s) 948
EaeI YGGCCR 1 cut(s) 115
Eam1104I CTCTTC 2 cut(s) 546, 762
EarI CTCTTC 2 cut(s) 546, 762
EciI GGCGGA 7 cut(s) 313, 484, 517, 574, 700, 733, 790
Ecl136II GAGCTC 1 cut(s) 413
Eco105I TACGTA 2 cut(s) 1575, 2178
Eco130I CCWWGG 1 cut(s) 129
Eco24I GRGCYC 1 cut(s) 415
Eco47I GGWCC 1 cut(s) 939
Eco53kI GAGCTC 1 cut(s) 413
Eco57I CTGAAG 2 cut(s) 1682, 2285
Eco88I CYCGRG 2 cut(s) 1317, 1920
EcoICRI GAGCTC 1 cut(s) 413
EcoRII CCWGG 3 cut(s) 633, 1246, 1849
EcoT14I CCWWGG 1 cut(s) 129
EcoT38I GRGCYC 1 cut(s) 415
ErhI CCWWGG 1 cut(s) 129
Esp3I CGTCTC 2 cut(s) 107, 963
FalI AAGNNNNNCTT 3 cut(s) 29, 437, 469
FaqI GGGAC 1 cut(s) 904
FauI CCCGC 1 cut(s) 253
FauNDI CATATG 2 cut(s) 467, 683
FbaI TGATCA 1 cut(s) 876
Fnu4HI GCNGC 7 cut(s) 200, 531, 747, 895, 931, 1314, 1917
FokI GGATG 3 cut(s) 307, 1568, 2171
FriOI GRGCYC 1 cut(s) 415
Fsp4HI GCNGC 7 cut(s) 200, 531, 747, 895, 931, 1314, 1917
FspAI RTGCGCAY 2 cut(s) 1437, 2040
FspBI CTAG 2 cut(s) 1457, 2060
FspI TGCGCA 2 cut(s) 1437, 2040
GlaI GCGC 4 cut(s) 859, 934, 1437, 2040
GluI GCNGC 7 cut(s) 200, 531, 747, 895, 931, 1314, 1917
GsaI CCCAGC 1 cut(s) 453
GsuI CTGGAG 3 cut(s) 449, 617, 833
HaeII RGCGCY 1 cut(s) 861
HaeIII GGCC 4 cut(s) 117, 872, 1322, 1925
HapII CCGG 3 cut(s) 391, 408, 438
HgaI GACGC 2 cut(s) 391, 413
HhaI GCGC 4 cut(s) 860, 935, 1438, 2041
Hin1I GRCGYC 2 cut(s) 383, 405
Hin6I GCGC 4 cut(s) 858, 933, 1436, 2039
HinP1I GCGC 4 cut(s) 858, 933, 1436, 2039
HincII GTYRAC 2 cut(s) 1200, 1803
HindII GTYRAC 2 cut(s) 1200, 1803
HinfI GANTC 8 cut(s) 593, 809, 1188, 1346, 1561, 1791, 1949, 2164
HpaII CCGG 3 cut(s) 391, 408, 438
HphI GGTGA 7 cut(s) 325, 490, 601, 706, 817, 1657, 2260
Hpy166II GTNNAC 9 cut(s) 580, 796, 942, 1200, 1417, 1648, 1803, 2020, 2251
Hpy188III TCNNGA 7 cut(s) 37, 850, 880, 1153, 1613, 1756, 2216
Hpy8I GTNNAC 9 cut(s) 580, 796, 942, 1200, 1417, 1648, 1803, 2020, 2251
Hpy99I CGWCG 2 cut(s) 385, 407
HpyAV CCTTC 2 cut(s) 29, 128
HpyCH4IV ACGT 3 cut(s) 986, 1574, 2177
HpyF10VI GCNNNNNNNGC 2 cut(s) 1319, 1922
HpyF3I CTNAG 9 cut(s) 960, 1132, 1330, 1511, 1565, 1735, 1933, 2114, 2168
HpySE526I ACGT 3 cut(s) 986, 1574, 2177
Hsp92I GRCGYC 2 cut(s) 383, 405
HspAI GCGC 4 cut(s) 858, 933, 1436, 2039
Ksp22I TGATCA 1 cut(s) 876
LmnI GCTCC 8 cut(s) 410, 624, 636, 840, 852, 1045, 1625, 2228
Lsp1109I GCAGC 4 cut(s) 211, 906, 1325, 1928
LweI GCATC 3 cut(s) 56, 1590, 2193
MaeI CTAG 2 cut(s) 1457, 2060
MaeII ACGT 3 cut(s) 986, 1574, 2177
MaeIII GTNAC 4 cut(s) 178, 331, 1670, 2273
MfeI CAATTG 2 cut(s) 1500, 2103
MflI RGATCY 3 cut(s) 158, 1693, 2296
MhlI GDGCHC 2 cut(s) 373, 415
MlsI TGGCCA 1 cut(s) 117
MluNI TGGCCA 1 cut(s) 117
MlyI GAGTC 2 cut(s) 587, 803
MmeI TCCRAC 3 cut(s) 193, 624, 840
Mox20I TGGCCA 1 cut(s) 117
MroXI GAANNNNTTC 6 cut(s) 1143, 1385, 1695, 1746, 1988, 2298
MscI TGGCCA 1 cut(s) 117
MseI TTAA 8 cut(s) 219, 1014, 1479, 1484, 1494, 2082, 2087, 2097
MslI CAYNNNNRTG 1 cut(s) 1100
Msp20I TGGCCA 1 cut(s) 117
MspA1I CMGCKG 2 cut(s) 1174, 1777
MspI CCGG 3 cut(s) 391, 408, 438
MspR9I CCNGG 4 cut(s) 439, 635, 1248, 1851
MunI CAATTG 2 cut(s) 1500, 2103
MvaI CCWGG 3 cut(s) 635, 1248, 1851
MwoI GCNNNNNNNGC 2 cut(s) 1319, 1922
NciI CCSGG 1 cut(s) 439
NdeI CATATG 2 cut(s) 467, 683
NlaIV GGNNCC 4 cut(s) 160, 267, 1110, 1713
NmuCI GTSAC 4 cut(s) 178, 331, 1670, 2273
NsbI TGCGCA 2 cut(s) 1437, 2040
NspI RCATGY 4 cut(s) 1277, 1436, 1880, 2039
PaeI GCATGC 2 cut(s) 1277, 1880
PagI TCATGA 3 cut(s) 879, 1152, 1755
PaqCI CACCTGC 2 cut(s) 466, 682
PcsI WCGNNNNNNNCGW 2 cut(s) 1416, 2019
PdmI GAANNNNTTC 6 cut(s) 1143, 1385, 1695, 1746, 1988, 2298
PfeI GAWTC 6 cut(s) 1188, 1346, 1561, 1791, 1949, 2164
PflMI CCANNNNNTGG 1 cut(s) 175
PfoI TCCNGGA 1 cut(s) 437
PkrI GCNGC 7 cut(s) 201, 532, 748, 896, 932, 1315, 1918
PleI GAGTC 2 cut(s) 587, 803
PpsI GAGTC 2 cut(s) 587, 803
Ppu21I YACGTR 2 cut(s) 1575, 2178
PshBI ATTAAT 2 cut(s) 1479, 2082
Psp124BI GAGCTC 1 cut(s) 415
Psp6I CCWGG 3 cut(s) 633, 1246, 1849
PspFI CCCAGC 1 cut(s) 449
PspGI CCWGG 3 cut(s) 633, 1246, 1849
PspN4I GGNNCC 4 cut(s) 160, 267, 1110, 1713
PspPI GGNCC 3 cut(s) 939, 1320, 1923
PstI CTGCAG 2 cut(s) 1315, 1918
PsuI RGATCY 3 cut(s) 158, 1693, 2296
PvuII CAGCTG 2 cut(s) 1174, 1777
RsaI GTAC 3 cut(s) 887, 1573, 2176
RsaNI GTAC 3 cut(s) 886, 1572, 2175
RseI CAYNNNNRTG 1 cut(s) 1100
SacI GAGCTC 1 cut(s) 415
SaqAI TTAA 8 cut(s) 219, 1014, 1479, 1484, 1494, 2082, 2087, 2097
SatI GCNGC 7 cut(s) 200, 531, 747, 895, 931, 1314, 1917
Sau96I GGNCC 3 cut(s) 939, 1320, 1923
SchI GAGTC 2 cut(s) 587, 803
ScrFI CCNGG 4 cut(s) 439, 635, 1248, 1851
SduI GDGCHC 2 cut(s) 373, 415
SfaNI GCATC 3 cut(s) 56, 1590, 2193
SfcI CTRYAG 2 cut(s) 1311, 1914
SinI GGWCC 1 cut(s) 939
SmiMI CAYNNNNRTG 1 cut(s) 1100
SnaBI TACGTA 2 cut(s) 1575, 2178
SphI GCATGC 2 cut(s) 1277, 1880
SspMI CTAG 2 cut(s) 1457, 2060
SstI GAGCTC 1 cut(s) 415
StyD4I CCNGG 4 cut(s) 437, 633, 1246, 1849
StyI CCWWGG 1 cut(s) 129
TaiI ACGT 3 cut(s) 989, 1577, 2180
TaqI TCGA 4 cut(s) 402, 504, 662, 720
TauI GCSGC 3 cut(s) 533, 749, 933
TfiI GAWTC 6 cut(s) 1188, 1346, 1561, 1791, 1949, 2164
Tru1I TTAA 8 cut(s) 219, 1014, 1479, 1484, 1494, 2082, 2087, 2097
Tru9I TTAA 8 cut(s) 219, 1014, 1479, 1484, 1494, 2082, 2087, 2097
TseFI GTSAC 4 cut(s) 178, 331, 1670, 2273
TseI GCWGC 4 cut(s) 199, 894, 1313, 1916
Tsp45I GTSAC 4 cut(s) 178, 331, 1670, 2273
TspGWI ACGGA 5 cut(s) 319, 565, 781, 1425, 2028
Van91I CCANNNNNTGG 1 cut(s) 175
VpaK11BI GGWCC 1 cut(s) 939
VspI ATTAAT 2 cut(s) 1479, 2082
XceI RCATGY 4 cut(s) 1277, 1436, 1880, 2039
XcmI CCANNNNNNNNNTGG 1 cut(s) 1003
XmnI GAANNNNTTC 6 cut(s) 1143, 1385, 1695, 1746, 1988, 2298
XspI CTAG 2 cut(s) 1457, 2060
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.