Rmu_sc0007209.1_g000001

GPI-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007209.1
OG0005399
Physical Location & Seq
Reverse (-)
1 .. 3144
3144 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1537 bp
atggccttctcaaaactgagcttattgtttgcctttgtgcttgttcacgccttcctcctccattcttctccagtatattgtaatgaagaagaagacgatcttttagaggaactcaacagtttcaggggatccctaaaccttacagccttcacagagaacgaaaaggccgattgtatagccgacgaaatctcggatgagctagaggatgagctatgctctagagcagctgaataccagattaaaccaggaaatggtcctcagttcccctccttcaccaagtccttaaccaagtgtgacatagacaacagcaccacaactgatgggatcattttgcctgtttgtgtgcacaatttggacccagatctggtgcgtaaaaattatactgaaaccaaatttgctcagtttctgaatgattccaagtatacaggagctgggcttggttctaaggatgattggatgatcgttgtgttgaccactgacgcacatagtgggagctttactggtggtgctgcttctttggttgcaactggcatggttcactacatggttgctttgttgttgggattgttcttgactcgcacgtgcgccaaaaggcctttacctgcgcgcccagaacagaagaagaaaagccattacgagcacgaggggaagaagcagagagcaatggcggacgacgacgacgacggcttcggagacttcactttcgtctccgccgccgccgccttcaatcccaatcccaccaccaccacccaacgacaatcggccgcctccgacgacgattggggcgatttcgtaaccgtcgccgcgcctccgatcaaaacccccgtcgttaacggattcgcacccgccccggcccaaacaccgcccgacccgttcgggtttttcaacgtaactaacggcgccgctgcggcgccgttaacgtcagccgggtcggatccaatccgggccgagacgaaggccgaaggggtcagtaagacgaggcagtggaagaagccgatgggagctttgccgttgtcgttgtttggggaagaggagagagacgagcaggtgaatcaggatttgactgagaatgagggatttgggaaaaagggatcgaatttgaatgggattagcggtgggattgatgatttgatatcgaatttgtatgtccagaacggttcgaatcgggattctggttcgggtggtattgattttagcaagagcgggttgaatttgaggtcgaatggggtggattcgaagcttggtggtttgattccgagccaaaatgggaagctggatggtggtgatttgagttcgaatggtttgaaattttttgagggtgtagatttgaatgggagctcaaattcaagtgtgttgaagatgaatgtggaagaagggaatggggattttgacgaggacgatgatgatggttgggaattcaaagctgctgaaccagagaactgggttggcaatgagaattctaaggaacggggaattttggaagtagcagagtcaaaagcagaatcaagtacacttgtaaacgggggaatacaggcgg
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

512

Amino Acids

54.88

Weight (kDa)

4.5

Isoelectric Point (pI)

29.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g19230 PF25884 31 - 157 1.5e-50 Uncharacterized GPI-anchored protein At5g19230-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1036
Acc36I ACCTGC 2 cut(s) 610, 1036
AccB1I GGYRCC 2 cut(s) 899, 910
AccB7I CCANNNNNTGG 1 cut(s) 251
AccBSI CCGCTC 1 cut(s) 1203
AccI GTMKAC 1 cut(s) 422
AccII CGCG 2 cut(s) 607, 806
AclWI GGATC 6 cut(s) 123, 136, 332, 929, 942, 1099
AcoI YGGCCR 1 cut(s) 762
AcsI RAATTY 9 cut(s) 392, 1096, 1138, 1210, 1307, 1342, 1415, 1456, 1473
AcvI CACGTG 1 cut(s) 582
AcyI GRCGYC 2 cut(s) 900, 911
AdeI CACNNNGTG 1 cut(s) 488
AfaI GTAC 1 cut(s) 1510
AfiI CCNNNNNNNGG 2 cut(s) 251, 364
AgsI TTSAA 9 cut(s) 727, 886, 1102, 1210, 1306, 1330, 1347, 1357, 1420
AjnI CCWGG 1 cut(s) 244
Alw21I GWGCWC 3 cut(s) 348, 642, 1340
Alw26I GTCTC 4 cut(s) 687, 712, 944, 1032
Alw44I GTGCAC 1 cut(s) 344
AlwI GGATC 6 cut(s) 123, 136, 332, 929, 942, 1099
AlwNI CAGNNNCTG 2 cut(s) 406, 431
AoxI GGCC 7 cut(s) 3, 165, 593, 762, 852, 945, 957
ApaLI GTGCAC 1 cut(s) 344
ApeKI GCWGC 4 cut(s) 224, 509, 905, 1424
ApoI RAATTY 9 cut(s) 392, 1096, 1138, 1210, 1307, 1342, 1415, 1456, 1473
ArsI GACNNNNNNTTYG 6 cut(s) 671, 686, 703, 718, 810, 842
AspLEI GCGC 6 cut(s) 587, 607, 609, 808, 902, 913
AspS9I GGNCC 4 cut(s) 254, 355, 853, 945
AsuC2I CCSGG 3 cut(s) 851, 928, 944
AsuHPI GGTGA 3 cut(s) 265, 1060, 1295
AsuII TTCGAA 3 cut(s) 1160, 1235, 1295
AvaII GGWCC 2 cut(s) 254, 355
BaeGI GKGCMC 1 cut(s) 348
BamHI GGATCC 2 cut(s) 128, 934
BanI GGYRCC 2 cut(s) 899, 910
BanII GRGCYC 1 cut(s) 1340
BauI CACGAG 1 cut(s) 641
BbrPI CACGTG 1 cut(s) 582
BbsI GAAGAC 1 cut(s) 99
Bbv12I GWGCWC 3 cut(s) 348, 642, 1340
BbvI GCAGC 4 cut(s) 236, 496, 892, 1411
BccI CCATC 4 cut(s) 314, 991, 1271, 1400
BceAI ACGGC 4 cut(s) 700, 898, 913, 994
BciT130I CCWGG 1 cut(s) 246
BcnI CCSGG 3 cut(s) 851, 928, 944
BcoDI GTCTC 4 cut(s) 687, 712, 944, 1032
BfaI CTAG 2 cut(s) 200, 219
BfoI RGCGCY 2 cut(s) 903, 914
BfuAI ACCTGC 2 cut(s) 610, 1036
BglI GCCNNNNNGGC 1 cut(s) 908
BglII AGATCT 1 cut(s) 361
Bme1390I CCNGG 4 cut(s) 246, 851, 928, 944
Bme18I GGWCC 2 cut(s) 254, 355
BmgT120I GGNCC 4 cut(s) 254, 355, 853, 945
BmiI GGNNCC 5 cut(s) 130, 357, 901, 912, 936
BmrFI CCNGG 4 cut(s) 246, 851, 928, 944
BmrI ACTGGG 1 cut(s) 1450
BmuI ACTGGG 1 cut(s) 1450
BpiI GAAGAC 1 cut(s) 99
BplI GAGNNNNNCTC 2 cut(s) 200, 232
BpmI CTGGAG 1 cut(s) 54
Bpu14I TTCGAA 3 cut(s) 1160, 1235, 1295
BpuMI CCSGG 3 cut(s) 851, 928, 944
BsaAI YACGTR 1 cut(s) 582
BsaHI GRCGYC 2 cut(s) 900, 911
BsaJI CCNNGG 1 cut(s) 849
Bsc4I CCNNNNNNNGG 2 cut(s) 251, 364
Bse1I ACTGG 4 cut(s) 71, 505, 532, 1445
Bse3DI GCAATG 2 cut(s) 669, 1456
BseBI CCWGG 1 cut(s) 246
BseDI CCNNGG 1 cut(s) 849
BseGI GGATG 5 cut(s) 199, 211, 454, 462, 1282
BseLI CCNNNNNNNGG 2 cut(s) 251, 364
BseMI GCAATG 2 cut(s) 669, 1456
BseMII CTCAG 4 cut(s) 8, 272, 413, 1056
BseNI ACTGG 4 cut(s) 71, 505, 532, 1445
BsePI GCGCGC 1 cut(s) 605
BseRI GAGGAG 2 cut(s) 47, 1046
BseSI GKGCMC 1 cut(s) 348
BseX3I CGGCCG 1 cut(s) 762
BseXI GCAGC 4 cut(s) 236, 496, 892, 1411
BseYI CCCAGC 1 cut(s) 431
Bsh1236I CGCG 2 cut(s) 607, 806
Bsh1285I CGRYCG 1 cut(s) 765
BshFI GGCC 7 cut(s) 5, 167, 595, 764, 854, 947, 959
BshNI GGYRCC 2 cut(s) 899, 910
BsiEI CGRYCG 1 cut(s) 765
BsiHKAI GWGCWC 3 cut(s) 348, 642, 1340
BsiSI CCGG 3 cut(s) 851, 927, 943
BslI CCNNNNNNNGG 2 cut(s) 251, 364
BsmAI GTCTC 4 cut(s) 687, 712, 944, 1032
BsmBI CGTCTC 3 cut(s) 712, 944, 1032
BsnI GGCC 7 cut(s) 5, 167, 595, 764, 854, 947, 959
Bsp119I TTCGAA 3 cut(s) 1160, 1235, 1295
Bsp1286I GDGCHC 3 cut(s) 348, 642, 1340
Bsp143I GATC 8 cut(s) 97, 128, 324, 361, 459, 813, 934, 1091
BspANI GGCC 7 cut(s) 5, 167, 595, 764, 854, 947, 959
BspCNI CTCAG 4 cut(s) 9, 271, 412, 1057
BspFNI CGCG 2 cut(s) 607, 806
BspLI GGNNCC 5 cut(s) 130, 357, 901, 912, 936
BspMI ACCTGC 2 cut(s) 610, 1036
BspPI GGATC 6 cut(s) 123, 136, 332, 929, 942, 1099
BspT104I TTCGAA 3 cut(s) 1160, 1235, 1295
BspT107I GGYRCC 2 cut(s) 899, 910
BsrBI CCGCTC 1 cut(s) 1203
BsrDI GCAATG 2 cut(s) 669, 1456
BsrI ACTGG 4 cut(s) 71, 505, 532, 1445
BssECI CCNNGG 1 cut(s) 849
BssHII GCGCGC 1 cut(s) 605
BssMI GATC 8 cut(s) 97, 128, 324, 361, 459, 813, 934, 1091
BssNAI GTATAC 1 cut(s) 423
BssNI GRCGYC 2 cut(s) 900, 911
BssSI CACGAG 1 cut(s) 641
Bst1107I GTATAC 1 cut(s) 423
Bst2BI CACGAG 1 cut(s) 641
Bst2UI CCWGG 1 cut(s) 246
Bst4CI ACNGT 3 cut(s) 119, 799, 1157
Bst6I CTCTTC 1 cut(s) 1023
BstACI GRCGYC 2 cut(s) 900, 911
BstBAI YACGTR 1 cut(s) 582
BstBI TTCGAA 3 cut(s) 1160, 1235, 1295
BstC8I GCNNGC 1 cut(s) 607
BstDEI CTNAG 6 cut(s) 17, 258, 399, 444, 1065, 1461
BstF5I GGATG 5 cut(s) 199, 211, 454, 462, 1282
BstFNI CGCG 2 cut(s) 607, 806
BstH2I RGCGCY 2 cut(s) 903, 914
BstHHI GCGC 6 cut(s) 587, 607, 609, 808, 902, 913
BstKTI GATC 8 cut(s) 100, 131, 327, 364, 462, 816, 937, 1094
BstMAI GTCTC 4 cut(s) 687, 712, 944, 1032
BstMBI GATC 8 cut(s) 97, 128, 324, 361, 459, 813, 934, 1091
BstMCI CGRYCG 1 cut(s) 765
BstMWI GCNNNNNNNGC 2 cut(s) 719, 908
BstNI CCWGG 1 cut(s) 246
BstSCI CCNGG 4 cut(s) 244, 849, 926, 942
BstSLI GKGCMC 1 cut(s) 348
BstUI CGCG 2 cut(s) 607, 806
BstV1I GCAGC 4 cut(s) 236, 496, 892, 1411
BstV2I GAAGAC 1 cut(s) 99
BstX2I RGATCY 3 cut(s) 128, 361, 934
BstXI CCANNNNNNTGG 1 cut(s) 1440
BstYI RGATCY 3 cut(s) 128, 361, 934
BstZ17I GTATAC 1 cut(s) 423
BstZI CGGCCG 1 cut(s) 762
BsuRI GGCC 7 cut(s) 5, 167, 595, 764, 854, 947, 959
BtsCI GGATG 5 cut(s) 199, 211, 454, 462, 1282
BtsI GCAGTG 1 cut(s) 989
BtsIMutI CAGTG 2 cut(s) 474, 989
BveI ACCTGC 2 cut(s) 610, 1036
Cac8I GCNNGC 1 cut(s) 607
CaiI CAGNNNCTG 2 cut(s) 406, 431
CfoI GCGC 6 cut(s) 587, 607, 609, 808, 902, 913
Cfr13I GGNCC 4 cut(s) 254, 355, 853, 945
CseI GACGC 1 cut(s) 488
Csp6I GTAC 1 cut(s) 1509
CviAII CATG 2 cut(s) 532, 544
CviQI GTAC 1 cut(s) 1509
DdeI CTNAG 6 cut(s) 17, 258, 399, 444, 1065, 1461
DinI GGCGCC 2 cut(s) 901, 912
DpnI GATC 8 cut(s) 99, 130, 326, 363, 461, 815, 936, 1093
DpnII GATC 8 cut(s) 97, 128, 324, 361, 459, 813, 934, 1091
DraIII CACNNNGTG 1 cut(s) 488
EaeI YGGCCR 1 cut(s) 762
EagI CGGCCG 1 cut(s) 762
Eam1104I CTCTTC 1 cut(s) 1023
EarI CTCTTC 1 cut(s) 1023
EciI GGCGGA 2 cut(s) 683, 700
Ecl136II GAGCTC 1 cut(s) 1338
EclXI CGGCCG 1 cut(s) 762
Eco147I AGGCCT 1 cut(s) 595
Eco24I GRGCYC 1 cut(s) 1340
Eco32I GATATC 1 cut(s) 1134
Eco47I GGWCC 2 cut(s) 254, 355
Eco52I CGGCCG 1 cut(s) 762
Eco53kI GAGCTC 1 cut(s) 1338
Eco72I CACGTG 1 cut(s) 582
EcoICRI GAGCTC 1 cut(s) 1338
EcoRI GAATTC 2 cut(s) 1415, 1456
EcoRII CCWGG 1 cut(s) 244
EcoRV GATATC 1 cut(s) 1134
EcoT38I GRGCYC 1 cut(s) 1340
EgeI GGCGCC 2 cut(s) 901, 912
EheI GGCGCC 2 cut(s) 901, 912
Esp3I CGTCTC 3 cut(s) 712, 944, 1032
FaeI CATG 2 cut(s) 535, 547
FalI AAGNNNNNCTT 2 cut(s) 84, 116
FatI CATG 2 cut(s) 531, 543
FauI CCCGC 2 cut(s) 853, 1196
FblI GTMKAC 1 cut(s) 422
FokI GGATG 5 cut(s) 206, 218, 461, 469, 1289
FriOI GRGCYC 1 cut(s) 1340
FspBI CTAG 2 cut(s) 200, 219
GlaI GCGC 6 cut(s) 586, 606, 608, 807, 901, 912
GsaI CCCAGC 1 cut(s) 435
GsuI CTGGAG 1 cut(s) 54
HaeII RGCGCY 2 cut(s) 903, 914
HaeIII GGCC 7 cut(s) 5, 167, 595, 764, 854, 947, 959
HapII CCGG 3 cut(s) 851, 927, 943
HgaI GACGC 1 cut(s) 488
HhaI GCGC 6 cut(s) 587, 607, 609, 808, 902, 913
Hin1I GRCGYC 2 cut(s) 900, 911
Hin1II CATG 2 cut(s) 535, 547
Hin6I GCGC 6 cut(s) 585, 605, 607, 806, 900, 911
HinP1I GCGC 6 cut(s) 585, 605, 607, 806, 900, 911
HincII GTYRAC 3 cut(s) 471, 832, 918
HindII GTYRAC 3 cut(s) 471, 832, 918
HindIII AAGCTT 1 cut(s) 1238
HpaI GTTAAC 2 cut(s) 832, 918
HpaII CCGG 3 cut(s) 851, 927, 943
HphI GGTGA 3 cut(s) 265, 1060, 1295
Hpy166II GTNNAC 9 cut(s) 46, 346, 423, 471, 538, 832, 918, 1511, 1519
Hpy188I TCNGA 7 cut(s) 193, 408, 692, 772, 813, 934, 1257
Hpy188III TCNNGA 5 cut(s) 219, 571, 1055, 1150, 1166
Hpy8I GTNNAC 9 cut(s) 46, 346, 423, 471, 538, 832, 918, 1511, 1519
Hpy99I CGWCG 9 cut(s) 185, 677, 680, 683, 686, 776, 779, 803, 830
HpyAV CCTTC 8 cut(s) 16, 61, 157, 280, 733, 949, 956, 1367
HpyCH4III ACNGT 3 cut(s) 119, 799, 1157
HpyCH4IV ACGT 3 cut(s) 581, 888, 920
HpyCH4V TGCA 2 cut(s) 346, 524
HpyF10VI GCNNNNNNNGC 2 cut(s) 719, 908
HpyF3I CTNAG 6 cut(s) 17, 258, 399, 444, 1065, 1461
HpySE526I ACGT 3 cut(s) 581, 888, 920
Hsp92I GRCGYC 2 cut(s) 900, 911
Hsp92II CATG 2 cut(s) 535, 547
HspAI GCGC 6 cut(s) 585, 605, 607, 806, 900, 911
KasI GGCGCC 2 cut(s) 899, 910
KspAI GTTAAC 2 cut(s) 832, 918
Kzo9I GATC 8 cut(s) 97, 128, 324, 361, 459, 813, 934, 1091
LmnI GCTCC 4 cut(s) 428, 492, 1001, 1335
Lsp1109I GCAGC 4 cut(s) 236, 496, 892, 1411
MaeI CTAG 2 cut(s) 200, 219
MaeII ACGT 3 cut(s) 581, 888, 920
MaeIII GTNAC 3 cut(s) 293, 793, 889
MalI GATC 8 cut(s) 99, 130, 326, 363, 461, 815, 936, 1093
MbiI CCGCTC 1 cut(s) 1203
MboI GATC 8 cut(s) 97, 128, 324, 361, 459, 813, 934, 1091
MflI RGATCY 3 cut(s) 128, 361, 934
MhlI GDGCHC 3 cut(s) 348, 642, 1340
Mly113I GGCGCC 2 cut(s) 900, 911
MlyI GAGTC 2 cut(s) 568, 1499
MmeI TCCRAC 2 cut(s) 795, 912
MseI TTAA 4 cut(s) 240, 284, 831, 917
MspA1I CMGCKG 2 cut(s) 227, 905
MspI CCGG 3 cut(s) 851, 927, 943
MspR9I CCNGG 4 cut(s) 246, 851, 928, 944
MvaI CCWGG 1 cut(s) 246
MvnI CGCG 2 cut(s) 607, 806
MwoI GCNNNNNNNGC 2 cut(s) 719, 908
NarI GGCGCC 2 cut(s) 900, 911
NciI CCSGG 3 cut(s) 851, 928, 944
NdeII GATC 8 cut(s) 97, 128, 324, 361, 459, 813, 934, 1091
NlaIII CATG 2 cut(s) 535, 547
NlaIV GGNNCC 5 cut(s) 130, 357, 901, 912, 936
NmeAIII GCCGAG 1 cut(s) 973
NmuCI GTSAC 1 cut(s) 293
NspV TTCGAA 3 cut(s) 1160, 1235, 1295
PaqCI CACCTGC 1 cut(s) 1036
PauI GCGCGC 1 cut(s) 605
PceI AGGCCT 1 cut(s) 595
PcsI WCGNNNNNNNCGW 3 cut(s) 165, 678, 783
PfeI GAWTC 8 cut(s) 413, 837, 1051, 1162, 1169, 1232, 1252, 1502
PflMI CCANNNNNTGG 1 cut(s) 251
PleI GAGTC 2 cut(s) 568, 1498
PluTI GGCGCC 2 cut(s) 903, 914
PmaCI CACGTG 1 cut(s) 582
PmlI CACGTG 1 cut(s) 582
PpsI GAGTC 2 cut(s) 568, 1498
Ppu21I YACGTR 1 cut(s) 582
Psp124BI GAGCTC 1 cut(s) 1340
Psp6I CCWGG 1 cut(s) 244
PspCI CACGTG 1 cut(s) 582
PspFI CCCAGC 1 cut(s) 431
PspGI CCWGG 1 cut(s) 244
PspN4I GGNNCC 5 cut(s) 130, 357, 901, 912, 936
PspPI GGNCC 4 cut(s) 254, 355, 853, 945
PstNI CAGNNNCTG 2 cut(s) 406, 431
PsuI RGATCY 3 cut(s) 128, 361, 934
PteI GCGCGC 1 cut(s) 605
PvuII CAGCTG 1 cut(s) 227
RsaI GTAC 1 cut(s) 1510
RsaNI GTAC 1 cut(s) 1509
SacI GAGCTC 1 cut(s) 1340
SaqAI TTAA 4 cut(s) 240, 284, 831, 917
Sau3AI GATC 8 cut(s) 97, 128, 324, 361, 459, 813, 934, 1091
Sau96I GGNCC 4 cut(s) 254, 355, 853, 945
SchI GAGTC 2 cut(s) 568, 1499
ScrFI CCNGG 4 cut(s) 246, 851, 928, 944
SduI GDGCHC 3 cut(s) 348, 642, 1340
SfoI GGCGCC 2 cut(s) 901, 912
SfuI TTCGAA 3 cut(s) 1160, 1235, 1295
SinI GGWCC 2 cut(s) 254, 355
SseBI AGGCCT 1 cut(s) 595
SspDI GGCGCC 2 cut(s) 899, 910
SspMI CTAG 2 cut(s) 200, 219
SstI GAGCTC 1 cut(s) 1340
StuI AGGCCT 1 cut(s) 595
StyD4I CCNGG 4 cut(s) 244, 849, 926, 942
TaaI ACNGT 3 cut(s) 119, 799, 1157
TaiI ACGT 3 cut(s) 584, 891, 923
TaqI TCGA 6 cut(s) 1094, 1136, 1160, 1220, 1235, 1295
TatI WGTACW 1 cut(s) 1508
TauI GCSGC 7 cut(s) 716, 719, 722, 767, 806, 905, 911
TfiI GAWTC 8 cut(s) 413, 837, 1051, 1162, 1169, 1232, 1252, 1502
Tru1I TTAA 4 cut(s) 240, 284, 831, 917
Tru9I TTAA 4 cut(s) 240, 284, 831, 917
TscAI CASTG 2 cut(s) 481, 989
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 4 cut(s) 224, 509, 905, 1424
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 2 cut(s) 99, 1376
TspGWI ACGGA 1 cut(s) 849
TspRI CASTG 2 cut(s) 481, 989
Van91I CCANNNNNTGG 1 cut(s) 251
VneI GTGCAC 1 cut(s) 344
VpaK11BI GGWCC 2 cut(s) 254, 355
XapI RAATTY 9 cut(s) 392, 1096, 1138, 1210, 1307, 1342, 1415, 1456, 1473
XbaI TCTAGA 1 cut(s) 218
XmiI GTMKAC 1 cut(s) 422
XspI CTAG 2 cut(s) 200, 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.