Rmu_sc0007618.1_g000021

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007618.1
OG0014857
Physical Location & Seq
Forward (+)
77509 .. 78650
1142 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0007618.1_g000021.1.cds

Sequence Viewer

Length: 501 bp
atgaatactgctacctgggctaccaagctccttgaggactaccatgcagcccatccggggtcaccaaagaagcaatcaaggcataaaactaagtggcagctgccacctcgaggaatgaagatggtgtgtgtctcgctgcaattgcacgtccctttccccatgctagatccgccttttcagatggagttggaagccatgagagcaggacttttactcatcattcaccaaggattagtcaatgttgatattgaaactgactgctctttggtgattgctgcactcaaaagtgacacggagaactactctgaagttgatgagactcctatgattatccgggatgctctctttgaggattcttgtgctagtactcgaggtgtagagcttcaatttcttgatcacacgacgagtgatggtgcacaagtcggtggctgtggtgatggtgacatgacctactacggtggccggaccctgacggtgaaggaggacggagggaccgactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

166

Amino Acids

18.27

Weight (kDa)

4.92

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 108
AciI CCGC 1 cut(s) 170
AclWI GGATC 1 cut(s) 161
AcoI YGGCCR 1 cut(s) 460
AcuI CTGAAG 1 cut(s) 327
AfaI GTAC 1 cut(s) 367
AfiI CCNNNNNNNGG 2 cut(s) 57, 110
AgsI TTSAA 2 cut(s) 251, 386
AjiI CACGTC 1 cut(s) 148
AjnI CCWGG 1 cut(s) 14
AluBI AGCT 3 cut(s) 28, 100, 382
AluI AGCT 3 cut(s) 28, 100, 382
Alw21I GWGCWC 1 cut(s) 418
Alw26I GTCTC 2 cut(s) 136, 311
Alw44I GTGCAC 1 cut(s) 414
AlwI GGATC 1 cut(s) 161
Ama87I CYCGRG 2 cut(s) 108, 369
AoxI GGCC 1 cut(s) 460
ApaLI GTGCAC 1 cut(s) 414
ApeKI GCWGC 5 cut(s) 47, 97, 100, 136, 275
AspS9I GGNCC 2 cut(s) 465, 492
AsuC2I CCSGG 2 cut(s) 57, 335
AsuHPI GGTGA 6 cut(s) 54, 215, 280, 446, 452, 487
AvaI CYCGRG 2 cut(s) 108, 369
AvaII GGWCC 2 cut(s) 465, 492
BaeGI GKGCMC 1 cut(s) 418
Bbv12I GWGCWC 1 cut(s) 418
BbvI GCAGC 5 cut(s) 59, 87, 109, 123, 262
BccI CCATC 5 cut(s) 60, 115, 175, 404, 431
BciT130I CCWGG 1 cut(s) 16
BclI TGATCA 1 cut(s) 394
BcnI CCSGG 2 cut(s) 57, 335
BcoDI GTCTC 2 cut(s) 136, 311
BfaI CTAG 3 cut(s) 164, 363, 499
BisI GCNGC 5 cut(s) 48, 98, 101, 137, 276
BlsI GCNGC 5 cut(s) 49, 99, 102, 138, 277
BmcAI AGTACT 1 cut(s) 367
Bme1390I CCNGG 3 cut(s) 16, 57, 335
Bme18I GGWCC 2 cut(s) 465, 492
BmeT110I CYCGRG 2 cut(s) 108, 369
BmgBI CACGTC 1 cut(s) 148
BmgT120I GGNCC 2 cut(s) 465, 492
BmiI GGNNCC 2 cut(s) 467, 493
BmrFI CCNGG 3 cut(s) 16, 57, 335
BmsI GCATC 1 cut(s) 328
BplI GAGNNNNNCTC 2 cut(s) 287, 319
BpuEI CTTGAG 1 cut(s) 53
BpuMI CCSGG 2 cut(s) 57, 335
BsaJI CCNNGG 3 cut(s) 15, 56, 226
Bsc4I CCNNNNNNNGG 2 cut(s) 57, 110
BseBI CCWGG 1 cut(s) 16
BseDI CCNNGG 3 cut(s) 15, 56, 226
BseGI GGATG 2 cut(s) 52, 343
BseLI CCNNNNNNNGG 2 cut(s) 57, 110
BseSI GKGCMC 1 cut(s) 418
BseXI GCAGC 5 cut(s) 59, 87, 109, 123, 262
BsgI GTGCAG 1 cut(s) 261
BshFI GGCC 1 cut(s) 462
BsiHKAI GWGCWC 1 cut(s) 418
BsiHKCI CYCGRG 2 cut(s) 108, 369
BsiSI CCGG 3 cut(s) 56, 334, 463
BslFI GGGAC 1 cut(s) 134
BslI CCNNNNNNNGG 2 cut(s) 57, 110
BsmAI GTCTC 2 cut(s) 136, 311
BsmFI GGGAC 1 cut(s) 134
BsnI GGCC 1 cut(s) 462
BsoBI CYCGRG 2 cut(s) 108, 369
Bsp1286I GDGCHC 1 cut(s) 418
Bsp143I GATC 2 cut(s) 166, 394
BspACI CCGC 1 cut(s) 170
BspANI GGCC 1 cut(s) 462
BspLI GGNNCC 2 cut(s) 467, 493
BspPI GGATC 1 cut(s) 161
BssECI CCNNGG 3 cut(s) 15, 56, 226
BssMI GATC 2 cut(s) 166, 394
BssT1I CCWWGG 1 cut(s) 226
Bst2UI CCWGG 1 cut(s) 16
Bst4CI ACNGT 2 cut(s) 458, 475
BstDEI CTNAG 1 cut(s) 90
BstEII GGTNACC 1 cut(s) 60
BstF5I GGATG 2 cut(s) 52, 343
BstKTI GATC 2 cut(s) 169, 397
BstMAI GTCTC 2 cut(s) 136, 311
BstMBI GATC 2 cut(s) 166, 394
BstMWI GCNNNNNNNGC 5 cut(s) 17, 79, 142, 169, 200
BstNI CCWGG 1 cut(s) 16
BstPI GGTNACC 1 cut(s) 60
BstSCI CCNGG 3 cut(s) 14, 55, 333
BstSLI GKGCMC 1 cut(s) 418
BstV1I GCAGC 5 cut(s) 59, 87, 109, 123, 262
BstX2I RGATCY 1 cut(s) 166
BstYI RGATCY 1 cut(s) 166
BsuRI GGCC 1 cut(s) 462
BtrI CACGTC 1 cut(s) 148
BtsCI GGATG 2 cut(s) 52, 343
Cfr13I GGNCC 2 cut(s) 465, 492
Csp6I GTAC 1 cut(s) 366
CviAII CATG 4 cut(s) 44, 160, 196, 445
CviJI RGCY 8 cut(s) 20, 28, 50, 100, 194, 382, 429, 462
CviKI_1 RGCY 8 cut(s) 20, 28, 50, 100, 194, 382, 429, 462
CviQI GTAC 1 cut(s) 366
DdeI CTNAG 1 cut(s) 90
DpnI GATC 2 cut(s) 168, 396
DpnII GATC 2 cut(s) 166, 394
EaeI YGGCCR 1 cut(s) 460
EciI GGCGGA 1 cut(s) 159
Eco130I CCWWGG 1 cut(s) 226
Eco47I GGWCC 2 cut(s) 465, 492
Eco57I CTGAAG 1 cut(s) 327
Eco88I CYCGRG 2 cut(s) 108, 369
Eco91I GGTNACC 1 cut(s) 60
EcoO65I GGTNACC 1 cut(s) 60
EcoRII CCWGG 1 cut(s) 14
EcoT14I CCWWGG 1 cut(s) 226
ErhI CCWWGG 1 cut(s) 226
FaeI CATG 4 cut(s) 47, 163, 199, 448
FaiI YATR 6 cut(s) 45, 84, 161, 197, 326, 446
FaqI GGGAC 1 cut(s) 134
FatI CATG 4 cut(s) 43, 159, 195, 444
FbaI TGATCA 1 cut(s) 394
Fnu4HI GCNGC 5 cut(s) 48, 98, 101, 137, 276
FokI GGATG 2 cut(s) 39, 350
Fsp4HI GCNGC 5 cut(s) 48, 98, 101, 137, 276
FspBI CTAG 3 cut(s) 164, 363, 499
GluI GCNGC 5 cut(s) 48, 98, 101, 137, 276
HaeIII GGCC 1 cut(s) 462
HapII CCGG 3 cut(s) 56, 334, 463
Hin1II CATG 4 cut(s) 47, 163, 199, 448
HinfI GANTC 2 cut(s) 319, 353
HpaII CCGG 3 cut(s) 56, 334, 463
HphI GGTGA 6 cut(s) 54, 215, 280, 446, 452, 487
Hpy166II GTNNAC 1 cut(s) 416
Hpy188I TCNGA 2 cut(s) 180, 307
Hpy188III TCNNGA 1 cut(s) 392
Hpy8I GTNNAC 1 cut(s) 416
Hpy99I CGWCG 1 cut(s) 406
HpyAV CCTTC 1 cut(s) 472
HpyCH4III ACNGT 2 cut(s) 458, 475
HpyCH4IV ACGT 1 cut(s) 147
HpyCH4V TGCA 5 cut(s) 47, 139, 145, 278, 416
HpyF10VI GCNNNNNNNGC 5 cut(s) 17, 79, 142, 169, 200
HpyF3I CTNAG 1 cut(s) 90
HpySE526I ACGT 1 cut(s) 147
Hsp92II CATG 4 cut(s) 47, 163, 199, 448
Ksp22I TGATCA 1 cut(s) 394
Kzo9I GATC 2 cut(s) 166, 394
LmnI GCTCC 1 cut(s) 33
LpnPI CCDG 6 cut(s) 28, 69, 189, 347, 476, 482
Lsp1109I GCAGC 5 cut(s) 59, 87, 109, 123, 262
LweI GCATC 1 cut(s) 328
MaeI CTAG 3 cut(s) 164, 363, 499
MaeII ACGT 1 cut(s) 147
MaeIII GTNAC 3 cut(s) 60, 287, 440
MalI GATC 2 cut(s) 168, 396
MboI GATC 2 cut(s) 166, 394
MboII GAAGA 1 cut(s) 130
MfeI CAATTG 1 cut(s) 140
MflI RGATCY 1 cut(s) 166
MhlI GDGCHC 1 cut(s) 418
MluCI AATT 2 cut(s) 140, 386
MlyI GAGTC 1 cut(s) 313
MmeI TCCRAC 1 cut(s) 168
MnlI CCTC 7 cut(s) 28, 104, 117, 343, 365, 475, 482
MspA1I CMGCKG 1 cut(s) 100
MspI CCGG 3 cut(s) 56, 334, 463
MspR9I CCNGG 3 cut(s) 16, 57, 335
MunI CAATTG 1 cut(s) 140
MvaI CCWGG 1 cut(s) 16
MwoI GCNNNNNNNGC 5 cut(s) 17, 79, 142, 169, 200
NciI CCSGG 2 cut(s) 57, 335
NdeII GATC 2 cut(s) 166, 394
NlaIII CATG 4 cut(s) 47, 163, 199, 448
NlaIV GGNNCC 2 cut(s) 467, 493
NmuCI GTSAC 3 cut(s) 60, 287, 440
PaeR7I CTCGAG 2 cut(s) 108, 369
PcsI WCGNNNNNNNCGW 1 cut(s) 492
PfeI GAWTC 1 cut(s) 353
PfoI TCCNGGA 1 cut(s) 333
PkrI GCNGC 5 cut(s) 49, 99, 102, 138, 277
PleI GAGTC 1 cut(s) 313
PpsI GAGTC 1 cut(s) 313
Psp6I CCWGG 1 cut(s) 14
PspEI GGTNACC 1 cut(s) 60
PspGI CCWGG 1 cut(s) 14
PspN4I GGNNCC 2 cut(s) 467, 493
PspPI GGNCC 2 cut(s) 465, 492
PspXI VCTCGAGB 2 cut(s) 108, 369
PsuI RGATCY 1 cut(s) 166
PvuII CAGCTG 1 cut(s) 100
RsaI GTAC 1 cut(s) 367
RsaNI GTAC 1 cut(s) 366
SatI GCNGC 5 cut(s) 48, 98, 101, 137, 276
Sau3AI GATC 2 cut(s) 166, 394
Sau96I GGNCC 2 cut(s) 465, 492
ScaI AGTACT 1 cut(s) 367
SchI GAGTC 1 cut(s) 313
ScrFI CCNGG 3 cut(s) 16, 57, 335
SduI GDGCHC 1 cut(s) 418
SetI ASST 8 cut(s) 17, 30, 102, 109, 150, 376, 384, 452
SfaNI GCATC 1 cut(s) 328
Sfr274I CTCGAG 2 cut(s) 108, 369
SinI GGWCC 2 cut(s) 465, 492
SlaI CTCGAG 2 cut(s) 108, 369
SmlI CTYRAG 3 cut(s) 32, 108, 369
SmoI CTYRAG 3 cut(s) 32, 108, 369
Sse9I AATT 2 cut(s) 140, 386
SsiI CCGC 1 cut(s) 170
SspMI CTAG 3 cut(s) 164, 363, 499
StyD4I CCNGG 3 cut(s) 14, 55, 333
StyI CCWWGG 1 cut(s) 226
TaaI ACNGT 2 cut(s) 458, 475
TaiI ACGT 1 cut(s) 150
TaqI TCGA 2 cut(s) 109, 370
TasI AATT 2 cut(s) 140, 386
TatI WGTACW 1 cut(s) 365
TfiI GAWTC 1 cut(s) 353
TseFI GTSAC 3 cut(s) 60, 287, 440
TseI GCWGC 5 cut(s) 47, 97, 100, 136, 275
Tsp45I GTSAC 3 cut(s) 60, 287, 440
TspDTI ATGAA 2 cut(s) 17, 131
TspGWI ACGGA 2 cut(s) 308, 501
VneI GTGCAC 1 cut(s) 414
VpaK11BI GGWCC 2 cut(s) 465, 492
XhoI CTCGAG 2 cut(s) 108, 369
XspI CTAG 3 cut(s) 164, 363, 499
ZrmI AGTACT 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.