Rmu_sc0008418.1_g000004

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008418.1
OG0005547
Physical Location & Seq
Reverse (-)
14509 .. 15213
705 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008418.1_g000004.1.cds

Sequence Viewer

Length: 705 bp
atgagcggtggttctgagcggtctatcatcggctttctagctattaccatggcaggaatcttttttttcaccgatgtgaacagatatgttaacaatccccaccactcatacattcacgtaacccatgcttcgctcgccgagttcaataggatcaccaccgccaccatcaacacccttcactataatctcgtactccacatcaccctccaaaaccccaacaaagatttcgacgtgtattatgataatattgtagtcatggcttattatcaaaatcagaggtttattatggagagctattcacatgtagtttaccaagggaagaacgacacaactcgtctcaaactgtcactcaagggacaacaggacatggacaacactcttggggtggttgaggaggggcatcaagagaattccaaggttgagcagccgcaccaccaatgcatacatgatgacaatggcgatgaaaaaaacaagaagtttagattatctgaatatgatatttttgtcaggctttatcttcaaaatactgcaatcccaaaccctaggtcgttgatcaggatgaattggatagggaactctcaattcacatgcaacttgaaaagggttccgttgatcacaacggctaattataaccctagtcatttcatgactgctgagtgcaattccgatttctatcgagtatttcatcctcctggcagtggttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

234

Amino Acids

27.06

Weight (kDa)

6.66

Isoelectric Point (pI)

36.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 630
AccBSI CCGCTC 2 cut(s) 6, 19
AciI CCGC 4 cut(s) 6, 19, 159, 428
AclWI GGATC 1 cut(s) 158
AcsI RAATTY 1 cut(s) 409
AfaI GTAC 1 cut(s) 192
AfiI CCNNNNNNNGG 1 cut(s) 698
AflIII ACRYGT 2 cut(s) 231, 301
AgsI TTSAA 3 cut(s) 145, 521, 598
AjiI CACGTC 1 cut(s) 232
AjnI CCWGG 1 cut(s) 691
AjuI GAANNNNNNNTTGG 2 cut(s) 209, 241
AleI CACNNNNGTG 1 cut(s) 74
AluBI AGCT 2 cut(s) 41, 294
AluI AGCT 2 cut(s) 41, 294
Alw26I GTCTC 1 cut(s) 341
AlwI GGATC 1 cut(s) 158
ApeKI GCWGC 1 cut(s) 424
ApoI RAATTY 1 cut(s) 409
ArsI GACNNNNNNTTYG 2 cut(s) 530, 562
AspA2I CCTAGG 1 cut(s) 542
AsuHPI GGTGA 3 cut(s) 61, 145, 193
AvrII CCTAGG 1 cut(s) 542
BbvI GCAGC 1 cut(s) 436
BccI CCATC 1 cut(s) 173
BceAI ACGGC 1 cut(s) 636
BciT130I CCWGG 1 cut(s) 693
BclI TGATCA 2 cut(s) 552, 612
BcoDI GTCTC 1 cut(s) 341
BfaI CTAG 3 cut(s) 38, 543, 636
BisI GCNGC 2 cut(s) 425, 428
BlnI CCTAGG 1 cut(s) 542
BlsI GCNGC 2 cut(s) 426, 429
Bme1390I CCNGG 1 cut(s) 693
BmgBI CACGTC 1 cut(s) 232
BmiI GGNNCC 1 cut(s) 606
BmrFI CCNGG 1 cut(s) 693
BmsI GCATC 1 cut(s) 409
BpuEI CTTGAG 1 cut(s) 335
BsaAI YACGTR 1 cut(s) 118
BsaBI GATNNNNATC 1 cut(s) 672
BsaJI CCNNGG 4 cut(s) 48, 313, 414, 542
Bsc4I CCNNNNNNNGG 1 cut(s) 698
Bse8I GATNNNNATC 1 cut(s) 672
BseBI CCWGG 1 cut(s) 693
BseDI CCNNGG 4 cut(s) 48, 313, 414, 542
BseGI GGATG 2 cut(s) 564, 685
BseJI GATNNNNATC 1 cut(s) 672
BseLI CCNNNNNNNGG 1 cut(s) 698
BseMII CTCAG 2 cut(s) 6, 645
BseRI GAGGAG 1 cut(s) 407
BseXI GCAGC 1 cut(s) 436
BslFI GGGAC 1 cut(s) 369
BslI CCNNNNNNNGG 1 cut(s) 698
BsmAI GTCTC 1 cut(s) 341
BsmBI CGTCTC 1 cut(s) 341
BsmFI GGGAC 1 cut(s) 369
Bsp143I GATC 3 cut(s) 150, 552, 612
Bsp19I CCATGG 1 cut(s) 48
BspACI CCGC 4 cut(s) 6, 19, 159, 428
BspCNI CTCAG 2 cut(s) 7, 646
BspHI TCATGA 1 cut(s) 645
BspLI GGNNCC 1 cut(s) 606
BspPI GGATC 1 cut(s) 158
BsrBI CCGCTC 2 cut(s) 6, 19
BssECI CCNNGG 4 cut(s) 48, 313, 414, 542
BssMI GATC 3 cut(s) 150, 552, 612
BssT1I CCWWGG 4 cut(s) 48, 313, 414, 542
Bst2UI CCWGG 1 cut(s) 693
Bst4CI ACNGT 1 cut(s) 345
BstBAI YACGTR 1 cut(s) 118
BstC8I GCNNGC 1 cut(s) 135
BstDEI CTNAG 2 cut(s) 15, 654
BstDSI CCRYGG 1 cut(s) 48
BstF5I GGATG 2 cut(s) 564, 685
BstKTI GATC 3 cut(s) 153, 555, 615
BstMAI GTCTC 1 cut(s) 341
BstMBI GATC 3 cut(s) 150, 552, 612
BstMWI GCNNNNNNNGC 1 cut(s) 134
BstNI CCWGG 1 cut(s) 693
BstNSI RCATGY 2 cut(s) 305, 591
BstSCI CCNGG 1 cut(s) 691
BstV1I GCAGC 1 cut(s) 436
BtgI CCRYGG 1 cut(s) 48
BtgZI GCGATG 1 cut(s) 474
BtrI CACGTC 1 cut(s) 232
BtsCI GGATG 2 cut(s) 564, 685
BtsI GCAGTG 1 cut(s) 703
BtsIMutI CAGTG 1 cut(s) 703
Cac8I GCNNGC 1 cut(s) 135
CciI TCATGA 1 cut(s) 645
Csp6I GTAC 1 cut(s) 191
CviAII CATG 8 cut(s) 49, 125, 256, 302, 367, 446, 588, 646
CviJI RGCY 7 cut(s) 33, 41, 260, 294, 427, 511, 623
CviKI_1 RGCY 7 cut(s) 33, 41, 260, 294, 427, 511, 623
CviQI GTAC 1 cut(s) 191
DdeI CTNAG 2 cut(s) 15, 654
DpnI GATC 3 cut(s) 152, 554, 614
DpnII GATC 3 cut(s) 150, 552, 612
Eco130I CCWWGG 4 cut(s) 48, 313, 414, 542
EcoRI GAATTC 1 cut(s) 409
EcoRII CCWGG 1 cut(s) 691
EcoT14I CCWWGG 4 cut(s) 48, 313, 414, 542
EcoT22I ATGCAT 1 cut(s) 443
ErhI CCWWGG 4 cut(s) 48, 313, 414, 542
Esp3I CGTCTC 1 cut(s) 341
FaeI CATG 8 cut(s) 52, 128, 259, 305, 370, 449, 591, 649
FaqI GGGAC 1 cut(s) 369
FatI CATG 8 cut(s) 48, 124, 255, 301, 366, 445, 587, 645
FbaI TGATCA 2 cut(s) 552, 612
Fnu4HI GCNGC 2 cut(s) 425, 428
FokI GGATG 2 cut(s) 571, 672
Fsp4HI GCNGC 2 cut(s) 425, 428
FspBI CTAG 3 cut(s) 38, 543, 636
GluI GCNGC 2 cut(s) 425, 428
Hin1II CATG 8 cut(s) 52, 128, 259, 305, 370, 449, 591, 649
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HinfI GANTC 1 cut(s) 57
HpaI GTTAAC 1 cut(s) 91
HphI GGTGA 3 cut(s) 61, 145, 193
Hpy166II GTNNAC 3 cut(s) 79, 91, 310
Hpy188I TCNGA 4 cut(s) 16, 276, 490, 667
Hpy188III TCNNGA 3 cut(s) 404, 556, 646
Hpy8I GTNNAC 3 cut(s) 79, 91, 310
Hpy99I CGWCG 1 cut(s) 233
HpyAV CCTTC 1 cut(s) 185
HpyCH4III ACNGT 1 cut(s) 345
HpyCH4IV ACGT 2 cut(s) 117, 231
HpyCH4V TGCA 4 cut(s) 441, 530, 591, 660
HpyF10VI GCNNNNNNNGC 1 cut(s) 134
HpyF3I CTNAG 2 cut(s) 15, 654
HpySE526I ACGT 2 cut(s) 117, 231
Hsp92II CATG 8 cut(s) 52, 128, 259, 305, 370, 449, 591, 649
Ksp22I TGATCA 2 cut(s) 552, 612
KspAI GTTAAC 1 cut(s) 91
Kzo9I GATC 3 cut(s) 150, 552, 612
LpnPI CCDG 5 cut(s) 39, 347, 493, 541, 678
Lsp1109I GCAGC 1 cut(s) 436
LweI GCATC 1 cut(s) 409
MaeI CTAG 3 cut(s) 38, 543, 636
MaeII ACGT 2 cut(s) 117, 231
MaeIII GTNAC 2 cut(s) 118, 345
MalI GATC 3 cut(s) 152, 554, 614
MbiI CCGCTC 2 cut(s) 6, 19
MboI GATC 3 cut(s) 150, 552, 612
MboII GAAGA 2 cut(s) 331, 509
MluCI AATT 5 cut(s) 409, 562, 581, 625, 661
MnlI CCTC 5 cut(s) 215, 270, 385, 388, 699
Mph1103I ATGCAT 1 cut(s) 443
MseI TTAA 1 cut(s) 90
MslI CAYNNNNRTG 1 cut(s) 74
MspR9I CCNGG 1 cut(s) 693
MvaI CCWGG 1 cut(s) 693
MwoI GCNNNNNNNGC 1 cut(s) 134
NcoI CCATGG 1 cut(s) 48
NdeII GATC 3 cut(s) 150, 552, 612
NlaIII CATG 8 cut(s) 52, 128, 259, 305, 370, 449, 591, 649
NlaIV GGNNCC 1 cut(s) 606
NmeAIII GCCGAG 1 cut(s) 163
NmuCI GTSAC 1 cut(s) 345
NsiI ATGCAT 1 cut(s) 443
NspI RCATGY 2 cut(s) 305, 591
OliI CACNNNNGTG 1 cut(s) 74
PagI TCATGA 1 cut(s) 645
PciI ACATGT 1 cut(s) 301
PfeI GAWTC 1 cut(s) 57
PkrI GCNGC 2 cut(s) 426, 429
Ppu21I YACGTR 1 cut(s) 118
PscI ACATGT 1 cut(s) 301
PsiI TTATAA 1 cut(s) 630
Psp6I CCWGG 1 cut(s) 691
PspGI CCWGG 1 cut(s) 691
PspN4I GGNNCC 1 cut(s) 606
RsaI GTAC 1 cut(s) 192
RsaNI GTAC 1 cut(s) 191
RseI CAYNNNNRTG 1 cut(s) 74
SaqAI TTAA 1 cut(s) 90
SatI GCNGC 2 cut(s) 425, 428
Sau3AI GATC 3 cut(s) 150, 552, 612
ScrFI CCNGG 1 cut(s) 693
SetI ASST 7 cut(s) 43, 120, 234, 281, 296, 420, 548
SfaNI GCATC 1 cut(s) 409
SmiMI CAYNNNNRTG 1 cut(s) 74
SmlI CTYRAG 1 cut(s) 350
SmoI CTYRAG 1 cut(s) 350
Sse9I AATT 5 cut(s) 409, 562, 581, 625, 661
SsiI CCGC 4 cut(s) 6, 19, 159, 428
SspI AATATT 1 cut(s) 247
SspMI CTAG 3 cut(s) 38, 543, 636
StyD4I CCNGG 1 cut(s) 691
StyI CCWWGG 4 cut(s) 48, 313, 414, 542
TaaI ACNGT 1 cut(s) 345
TaiI ACGT 2 cut(s) 120, 234
TaqI TCGA 2 cut(s) 228, 676
TasI AATT 5 cut(s) 409, 562, 581, 625, 661
TauI GCSGC 1 cut(s) 430
TfiI GAWTC 1 cut(s) 57
Tru1I TTAA 1 cut(s) 90
Tru9I TTAA 1 cut(s) 90
TscAI CASTG 1 cut(s) 703
TseFI GTSAC 1 cut(s) 345
TseI GCWGC 1 cut(s) 424
Tsp45I GTSAC 1 cut(s) 345
TspDTI ATGAA 4 cut(s) 477, 575, 634, 674
TspGWI ACGGA 1 cut(s) 597
TspRI CASTG 1 cut(s) 703
XapI RAATTY 1 cut(s) 409
XceI RCATGY 2 cut(s) 305, 591
XmaJI CCTAGG 1 cut(s) 542
XspI CTAG 3 cut(s) 38, 543, 636
Zsp2I ATGCAT 1 cut(s) 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.