Rmu_sc0010238.1_g000009

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0010238.1
OG0005547
Physical Location & Seq
Reverse (-)
59442 .. 60143
702 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0010238.1_g000009.1.cds

Sequence Viewer

Length: 702 bp
atgagcggtagttttaaggggtccttcatcggctttctagctattgccatggcaacaatcttttttttcaccgatgtgaacagatatgttgacaatccccaccactcatacattcacgtaactcatgcttcgctcgcggagttcaataggatcaccaccgccgccatcaacacccttcactataatctcgtactccacatcacccttcaaaaccccaacaaagatttcgacgtctattatgataacattgtagtcatggcttattatcaaaatcagaggtttattatggagagctattcacatgtagtttaccaagggaagaacgaaacaactcgtctcacactgtcactcaagggacaacaggacatggacaacactcttggggtggttgaggaggggcatcaagagaattccaaggttgagcagacgcaggaccaatgcatacatgatgacaatggcaatgaaaataacaagaagtttagatcaactgaatatgatatttttgtcaggctttatcttcaaaacactgcaatcccaaaccctaggtcgttgatcaggatgaattggatagggaactctcaattcacatgcaacttgaaaagggttccgttgatcacaacggctaattatggtagtcattacatgaccgctgaatgcaattccgatttctatcgagtatttcatcctcccggcagtggttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

233

Amino Acids

26.86

Weight (kDa)

6.24

Isoelectric Point (pI)

37.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 234
AccBSI CCGCTC 1 cut(s) 6
AccII CGCG 1 cut(s) 137
AciI CCGC 5 cut(s) 6, 137, 159, 162, 648
AclWI GGATC 1 cut(s) 158
AcsI RAATTY 1 cut(s) 409
AcyI GRCGYC 1 cut(s) 231
AfaI GTAC 1 cut(s) 192
AfiI CCNNNNNNNGG 1 cut(s) 695
AflIII ACRYGT 1 cut(s) 301
AgsI TTSAA 4 cut(s) 145, 209, 521, 598
AjuI GAANNNNNNNTTGG 2 cut(s) 209, 241
AleI CACNNNNGTG 1 cut(s) 74
AluBI AGCT 2 cut(s) 41, 294
AluI AGCT 2 cut(s) 41, 294
Alw26I GTCTC 1 cut(s) 341
AlwI GGATC 1 cut(s) 158
ApoI RAATTY 1 cut(s) 409
ArsI GACNNNNNNTTYG 2 cut(s) 530, 562
AspA2I CCTAGG 1 cut(s) 542
AspS9I GGNCC 2 cut(s) 21, 433
AsuC2I CCSGG 1 cut(s) 690
AsuHPI GGTGA 3 cut(s) 61, 145, 193
AvaII GGWCC 2 cut(s) 21, 433
AvrII CCTAGG 1 cut(s) 542
BccI CCATC 1 cut(s) 173
BceAI ACGGC 1 cut(s) 636
BclI TGATCA 2 cut(s) 552, 612
BcnI CCSGG 1 cut(s) 690
BcoDI GTCTC 1 cut(s) 341
BfaI CTAG 2 cut(s) 38, 543
BisI GCNGC 1 cut(s) 162
BlnI CCTAGG 1 cut(s) 542
BlsI GCNGC 1 cut(s) 163
Bme1390I CCNGG 1 cut(s) 690
Bme18I GGWCC 2 cut(s) 21, 433
BmgT120I GGNCC 2 cut(s) 21, 433
BmiI GGNNCC 2 cut(s) 22, 606
BmrFI CCNGG 1 cut(s) 690
BmsI GCATC 1 cut(s) 409
BpuEI CTTGAG 1 cut(s) 335
BpuMI CCSGG 1 cut(s) 690
BsaAI YACGTR 1 cut(s) 118
BsaBI GATNNNNATC 1 cut(s) 669
BsaHI GRCGYC 1 cut(s) 231
BsaJI CCNNGG 4 cut(s) 48, 313, 414, 542
Bsc4I CCNNNNNNNGG 1 cut(s) 695
Bse3DI GCAATG 1 cut(s) 466
Bse8I GATNNNNATC 1 cut(s) 669
BseDI CCNNGG 4 cut(s) 48, 313, 414, 542
BseGI GGATG 2 cut(s) 564, 682
BseJI GATNNNNATC 1 cut(s) 669
BseLI CCNNNNNNNGG 1 cut(s) 695
BseMI GCAATG 1 cut(s) 466
BseRI GAGGAG 1 cut(s) 407
Bsh1236I CGCG 1 cut(s) 137
BsiSI CCGG 1 cut(s) 690
BslFI GGGAC 1 cut(s) 369
BslI CCNNNNNNNGG 1 cut(s) 695
BsmAI GTCTC 1 cut(s) 341
BsmBI CGTCTC 1 cut(s) 341
BsmFI GGGAC 1 cut(s) 369
BsmI GAATGC 1 cut(s) 659
Bsp143I GATC 4 cut(s) 150, 482, 552, 612
Bsp19I CCATGG 1 cut(s) 48
BspACI CCGC 5 cut(s) 6, 137, 159, 162, 648
BspFNI CGCG 1 cut(s) 137
BspLI GGNNCC 2 cut(s) 22, 606
BspPI GGATC 1 cut(s) 158
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 1 cut(s) 466
BssECI CCNNGG 4 cut(s) 48, 313, 414, 542
BssMI GATC 4 cut(s) 150, 482, 552, 612
BssNI GRCGYC 1 cut(s) 231
BssT1I CCWWGG 4 cut(s) 48, 313, 414, 542
Bst4CI ACNGT 1 cut(s) 345
BstACI GRCGYC 1 cut(s) 231
BstBAI YACGTR 1 cut(s) 118
BstC8I GCNNGC 1 cut(s) 135
BstDSI CCRYGG 1 cut(s) 48
BstF5I GGATG 2 cut(s) 564, 682
BstFNI CGCG 1 cut(s) 137
BstKTI GATC 4 cut(s) 153, 485, 555, 615
BstMAI GTCTC 1 cut(s) 341
BstMBI GATC 4 cut(s) 150, 482, 552, 612
BstMWI GCNNNNNNNGC 1 cut(s) 134
BstNSI RCATGY 2 cut(s) 305, 591
BstSCI CCNGG 1 cut(s) 688
BstUI CGCG 1 cut(s) 137
BtgI CCRYGG 1 cut(s) 48
BtsCI GGATG 2 cut(s) 564, 682
BtsI GCAGTG 2 cut(s) 525, 700
BtsIMutI CAGTG 3 cut(s) 341, 525, 700
Cac8I GCNNGC 1 cut(s) 135
Cfr13I GGNCC 2 cut(s) 21, 433
CseI GACGC 1 cut(s) 436
Csp6I GTAC 1 cut(s) 191
CviAII CATG 8 cut(s) 49, 125, 256, 302, 367, 446, 588, 643
CviJI RGCY 6 cut(s) 33, 41, 260, 294, 511, 623
CviKI_1 RGCY 6 cut(s) 33, 41, 260, 294, 511, 623
CviQI GTAC 1 cut(s) 191
DpnI GATC 4 cut(s) 152, 484, 554, 614
DpnII GATC 4 cut(s) 150, 482, 552, 612
Eco130I CCWWGG 4 cut(s) 48, 313, 414, 542
Eco47I GGWCC 2 cut(s) 21, 433
EcoO109I RGGNCCY 1 cut(s) 21
EcoRI GAATTC 1 cut(s) 409
EcoT14I CCWWGG 4 cut(s) 48, 313, 414, 542
EcoT22I ATGCAT 1 cut(s) 443
ErhI CCWWGG 4 cut(s) 48, 313, 414, 542
Esp3I CGTCTC 1 cut(s) 341
FaeI CATG 8 cut(s) 52, 128, 259, 305, 370, 449, 591, 646
FalI AAGNNNNNCTT 2 cut(s) 8, 40
FaqI GGGAC 1 cut(s) 369
FatI CATG 8 cut(s) 48, 124, 255, 301, 366, 445, 587, 642
FbaI TGATCA 2 cut(s) 552, 612
Fnu4HI GCNGC 1 cut(s) 162
FokI GGATG 2 cut(s) 571, 669
Fsp4HI GCNGC 1 cut(s) 162
FspBI CTAG 2 cut(s) 38, 543
GluI GCNGC 1 cut(s) 162
HapII CCGG 1 cut(s) 690
HgaI GACGC 1 cut(s) 436
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 8 cut(s) 52, 128, 259, 305, 370, 449, 591, 646
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HpaII CCGG 1 cut(s) 690
HphI GGTGA 3 cut(s) 61, 145, 193
Hpy166II GTNNAC 3 cut(s) 79, 91, 310
Hpy188I TCNGA 2 cut(s) 276, 664
Hpy188III TCNNGA 2 cut(s) 404, 556
Hpy8I GTNNAC 3 cut(s) 79, 91, 310
Hpy99I CGWCG 1 cut(s) 233
HpyAV CCTTC 3 cut(s) 34, 185, 215
HpyCH4III ACNGT 1 cut(s) 345
HpyCH4IV ACGT 2 cut(s) 117, 231
HpyCH4V TGCA 4 cut(s) 441, 530, 591, 657
HpyF10VI GCNNNNNNNGC 1 cut(s) 134
HpySE526I ACGT 2 cut(s) 117, 231
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 8 cut(s) 52, 128, 259, 305, 370, 449, 591, 646
Ksp22I TGATCA 2 cut(s) 552, 612
Kzo9I GATC 4 cut(s) 150, 482, 552, 612
LpnPI CCDG 4 cut(s) 347, 416, 493, 541
LweI GCATC 1 cut(s) 409
MaeI CTAG 2 cut(s) 38, 543
MaeII ACGT 2 cut(s) 117, 231
MaeIII GTNAC 2 cut(s) 118, 345
MalI GATC 4 cut(s) 152, 484, 554, 614
MbiI CCGCTC 1 cut(s) 6
MboI GATC 4 cut(s) 150, 482, 552, 612
MboII GAAGA 2 cut(s) 331, 509
MluCI AATT 5 cut(s) 409, 562, 581, 625, 658
MnlI CCTC 4 cut(s) 270, 385, 388, 696
Mph1103I ATGCAT 1 cut(s) 443
MseI TTAA 1 cut(s) 15
MslI CAYNNNNRTG 1 cut(s) 74
MspA1I CMGCKG 1 cut(s) 650
MspI CCGG 1 cut(s) 690
MspR9I CCNGG 1 cut(s) 690
Mva1269I GAATGC 1 cut(s) 659
MvnI CGCG 1 cut(s) 137
MwoI GCNNNNNNNGC 1 cut(s) 134
NciI CCSGG 1 cut(s) 690
NcoI CCATGG 1 cut(s) 48
NdeII GATC 4 cut(s) 150, 482, 552, 612
NlaIII CATG 8 cut(s) 52, 128, 259, 305, 370, 449, 591, 646
NlaIV GGNNCC 2 cut(s) 22, 606
NmuCI GTSAC 1 cut(s) 345
NsiI ATGCAT 1 cut(s) 443
NspI RCATGY 2 cut(s) 305, 591
OliI CACNNNNGTG 1 cut(s) 74
PciI ACATGT 1 cut(s) 301
PctI GAATGC 1 cut(s) 659
PkrI GCNGC 1 cut(s) 163
Ppu21I YACGTR 1 cut(s) 118
PpuMI RGGWCCY 1 cut(s) 21
PscI ACATGT 1 cut(s) 301
Psp5II RGGWCCY 1 cut(s) 21
PspN4I GGNNCC 2 cut(s) 22, 606
PspPI GGNCC 2 cut(s) 21, 433
PspPPI RGGWCCY 1 cut(s) 21
RsaI GTAC 1 cut(s) 192
RsaNI GTAC 1 cut(s) 191
RseI CAYNNNNRTG 1 cut(s) 74
SaqAI TTAA 1 cut(s) 15
SatI GCNGC 1 cut(s) 162
Sau3AI GATC 4 cut(s) 150, 482, 552, 612
Sau96I GGNCC 2 cut(s) 21, 433
ScrFI CCNGG 1 cut(s) 690
SetI ASST 7 cut(s) 43, 120, 234, 281, 296, 420, 548
SfaNI GCATC 1 cut(s) 409
SinI GGWCC 2 cut(s) 21, 433
SmiMI CAYNNNNRTG 1 cut(s) 74
SmlI CTYRAG 1 cut(s) 350
SmoI CTYRAG 1 cut(s) 350
Sse9I AATT 5 cut(s) 409, 562, 581, 625, 658
SsiI CCGC 5 cut(s) 6, 137, 159, 162, 648
SspMI CTAG 2 cut(s) 38, 543
StyD4I CCNGG 1 cut(s) 688
StyI CCWWGG 4 cut(s) 48, 313, 414, 542
TaaI ACNGT 1 cut(s) 345
TaiI ACGT 2 cut(s) 120, 234
TaqI TCGA 2 cut(s) 228, 673
TasI AATT 5 cut(s) 409, 562, 581, 625, 658
TauI GCSGC 1 cut(s) 164
Tru1I TTAA 1 cut(s) 15
Tru9I TTAA 1 cut(s) 15
TscAI CASTG 3 cut(s) 348, 532, 700
TseFI GTSAC 1 cut(s) 345
Tsp45I GTSAC 1 cut(s) 345
TspDTI ATGAA 4 cut(s) 16, 477, 575, 671
TspGWI ACGGA 1 cut(s) 597
TspRI CASTG 3 cut(s) 348, 532, 700
VpaK11BI GGWCC 2 cut(s) 21, 433
XapI RAATTY 1 cut(s) 409
XceI RCATGY 2 cut(s) 305, 591
XmaJI CCTAGG 1 cut(s) 542
XspI CTAG 2 cut(s) 38, 543
ZraI GACGTC 1 cut(s) 232
Zsp2I ATGCAT 1 cut(s) 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.