Rroxscaffold_4G00278410

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005547
Physical Location & Seq
Reverse (-)
1418696 .. 1421773
3078 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00278410.1

Sequence Viewer

Length: 819 bp
ATGGCTTATTATCAAAATCAGAGGTTTATTATGGAGAGCTATTCACATGTAGTTTACCAAGGGAAGAACGACACAACTCGTCTCAAACTGTCACTCAAGGGACAACAGGACATGGACAACACTCTTGGGGTGGTTGAGGAGGGGCATCAAGAGAATTCCAAGGTTGAGCAGCCGCACCACCAATGCATACATGATGACAATGGCGATGAAAAAAACAAGAAGTTTAGATCATCTGAATATGATATTTTTGTCAGGCTTTATCTTCAAAATACTGCAATCCCAAACCCTAGGTCGTTGATCAGGATGAATTGGATAGGGAACTCTCAATTCACATGCAACTTGAAAAGGGTTCCGTTGATTACAACGGCTAATTATAACCCTAGTCATTTCATGACTGCTGAGTGCAATTCCGATTTCTATCGAGTATTTCATCCTCCTGGCAGTGTATCCGGTCTAGTTCAATCGTTCAGAAACAGTGGGGTAAGAGTTCTCCTCGACAGGATCAGTCCAGAATTCAGAAACGACGCTCTTCAAGTCGCTGGCTATGAAAAAAGTAAAGGCTTCAACTTTTGTGCTGCACAAACAGAATTGTTTGAGATCGGCAAGTCATCAGAAGAGGAGGTGCGCACTATGCCTTGCGTGATGAAAAGCATCCTAGCTATCAATTGGCTCACTTTCCCGAATAATATAGCTGTTCTCACTATAGATTACTGGCTTAAGATGACTATCACACACTGCTGTCAGTACTTGTTCATCCATAGCTTGAAGCACTGGCACCTAAGGAACATGATTATTATACTATCAGGATGGATGACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

272

Amino Acids

31.6

Weight (kDa)

8.12

Isoelectric Point (pI)

41.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 375
Acc16I TGCGCA 1 cut(s) 626
AccB1I GGYRCC 1 cut(s) 774
AciI CCGC 1 cut(s) 173
AclWI GGATC 1 cut(s) 509
AcsI RAATTY 2 cut(s) 154, 512
AfaI GTAC 1 cut(s) 746
AflII CTTAAG 1 cut(s) 716
AflIII ACRYGT 1 cut(s) 46
AgsI TTSAA 6 cut(s) 266, 343, 461, 533, 565, 766
AjnI CCWGG 1 cut(s) 436
AluBI AGCT 4 cut(s) 39, 659, 692, 762
AluI AGCT 4 cut(s) 39, 659, 692, 762
Alw26I GTCTC 1 cut(s) 86
AlwI GGATC 1 cut(s) 509
ApeKI GCWGC 2 cut(s) 169, 575
ApoI RAATTY 2 cut(s) 154, 512
ArsI GACNNNNNNTTYG 2 cut(s) 275, 307
AspA2I CCTAGG 1 cut(s) 287
AspLEI GCGC 1 cut(s) 627
AvrII CCTAGG 1 cut(s) 287
AxyI CCTNAGG 1 cut(s) 779
BanI GGYRCC 1 cut(s) 774
BbvI GCAGC 2 cut(s) 181, 562
BccI CCATC 1 cut(s) 801
BceAI ACGGC 1 cut(s) 381
BciT130I CCWGG 1 cut(s) 438
BciVI GTATCC 1 cut(s) 457
BclI TGATCA 1 cut(s) 297
BcoDI GTCTC 1 cut(s) 86
BfaI CTAG 4 cut(s) 288, 381, 455, 656
BfmI CTRYAG 1 cut(s) 702
BfrI CTTAAG 1 cut(s) 716
BfuI GTATCC 1 cut(s) 457
BisI GCNGC 3 cut(s) 170, 173, 576
BlnI CCTAGG 1 cut(s) 287
BlsI GCNGC 3 cut(s) 171, 174, 577
BmcAI AGTACT 1 cut(s) 746
Bme1390I CCNGG 1 cut(s) 438
BmiI GGNNCC 2 cut(s) 351, 776
BmrFI CCNGG 1 cut(s) 438
BmsI GCATC 2 cut(s) 154, 660
BplI GAGNNNNNCTC 2 cut(s) 477, 509
BpuEI CTTGAG 1 cut(s) 80
BsaBI GATNNNNATC 2 cut(s) 417, 725
BsaJI CCNNGG 3 cut(s) 58, 159, 287
BsaWI WCCGGW 1 cut(s) 449
Bse1I ACTGG 2 cut(s) 716, 776
Bse21I CCTNAGG 1 cut(s) 779
Bse8I GATNNNNATC 2 cut(s) 417, 725
BseBI CCWGG 1 cut(s) 438
BseDI CCNNGG 3 cut(s) 58, 159, 287
BseGI GGATG 6 cut(s) 309, 430, 651, 753, 812, 816
BseJI GATNNNNATC 2 cut(s) 417, 725
BseMII CTCAG 1 cut(s) 390
BseNI ACTGG 2 cut(s) 716, 776
BseRI GAGGAG 3 cut(s) 152, 482, 632
BseXI GCAGC 2 cut(s) 181, 562
BsgI GTGCAG 1 cut(s) 561
BshNI GGYRCC 1 cut(s) 774
BsiSI CCGG 1 cut(s) 450
BslFI GGGAC 1 cut(s) 114
BsmAI GTCTC 1 cut(s) 86
BsmBI CGTCTC 1 cut(s) 86
BsmFI GGGAC 1 cut(s) 114
Bsp143I GATC 4 cut(s) 227, 297, 501, 597
BspACI CCGC 1 cut(s) 173
BspCNI CTCAG 1 cut(s) 391
BspHI TCATGA 1 cut(s) 390
BspLI GGNNCC 2 cut(s) 351, 776
BspPI GGATC 1 cut(s) 509
BspQI GCTCTTC 1 cut(s) 534
BspT107I GGYRCC 1 cut(s) 774
BspTI CTTAAG 1 cut(s) 716
BsrI ACTGG 2 cut(s) 716, 776
BssECI CCNNGG 3 cut(s) 58, 159, 287
BssMI GATC 4 cut(s) 227, 297, 501, 597
BssT1I CCWWGG 3 cut(s) 58, 159, 287
Bst2UI CCWGG 1 cut(s) 438
Bst4CI ACNGT 2 cut(s) 90, 476
Bst6I CTCTTC 2 cut(s) 534, 609
BstAFI CTTAAG 1 cut(s) 716
BstC8I GCNNGC 1 cut(s) 541
BstDEI CTNAG 2 cut(s) 399, 779
BstF5I GGATG 6 cut(s) 309, 430, 651, 753, 812, 816
BstHHI GCGC 1 cut(s) 627
BstKTI GATC 4 cut(s) 230, 300, 504, 600
BstMAI GTCTC 1 cut(s) 86
BstMBI GATC 4 cut(s) 227, 297, 501, 597
BstMWI GCNNNNNNNGC 1 cut(s) 631
BstNI CCWGG 1 cut(s) 438
BstNSI RCATGY 2 cut(s) 50, 336
BstSCI CCNGG 1 cut(s) 436
BstSFI CTRYAG 1 cut(s) 702
BstV1I GCAGC 2 cut(s) 181, 562
Bsu36I CCTNAGG 1 cut(s) 779
BsuI GTATCC 1 cut(s) 457
BtgZI GCGATG 1 cut(s) 219
BtsCI GGATG 6 cut(s) 309, 430, 651, 753, 812, 816
BtsI GCAGTG 2 cut(s) 448, 733
BtsIMutI CAGTG 4 cut(s) 448, 481, 733, 769
Cac8I GCNNGC 1 cut(s) 541
CciI TCATGA 1 cut(s) 390
CfoI GCGC 1 cut(s) 627
CseI GACGC 1 cut(s) 533
Csp6I GTAC 1 cut(s) 745
CviAII CATG 6 cut(s) 47, 112, 191, 333, 391, 787
CviQI GTAC 1 cut(s) 745
DdeI CTNAG 2 cut(s) 399, 779
DpnI GATC 4 cut(s) 229, 299, 503, 599
DpnII GATC 4 cut(s) 227, 297, 501, 597
Eam1104I CTCTTC 2 cut(s) 534, 609
EarI CTCTTC 2 cut(s) 534, 609
Eco130I CCWWGG 3 cut(s) 58, 159, 287
Eco81I CCTNAGG 1 cut(s) 779
EcoRI GAATTC 2 cut(s) 154, 512
EcoRII CCWGG 1 cut(s) 436
EcoT14I CCWWGG 3 cut(s) 58, 159, 287
EcoT22I ATGCAT 1 cut(s) 188
ErhI CCWWGG 3 cut(s) 58, 159, 287
Esp3I CGTCTC 1 cut(s) 86
FaeI CATG 6 cut(s) 50, 115, 194, 336, 394, 790
FaqI GGGAC 1 cut(s) 114
FatI CATG 6 cut(s) 46, 111, 190, 332, 390, 786
FbaI TGATCA 1 cut(s) 297
Fnu4HI GCNGC 3 cut(s) 170, 173, 576
FokI GGATG 4 cut(s) 316, 417, 638, 740
Fsp4HI GCNGC 3 cut(s) 170, 173, 576
FspAI RTGCGCAY 1 cut(s) 626
FspBI CTAG 4 cut(s) 288, 381, 455, 656
FspI TGCGCA 1 cut(s) 626
GlaI GCGC 1 cut(s) 626
GluI GCNGC 3 cut(s) 170, 173, 576
HapII CCGG 1 cut(s) 450
HgaI GACGC 1 cut(s) 533
HhaI GCGC 1 cut(s) 627
Hin1II CATG 6 cut(s) 50, 115, 194, 336, 394, 790
Hin6I GCGC 1 cut(s) 625
HinP1I GCGC 1 cut(s) 625
HpaII CCGG 1 cut(s) 450
Hpy166II GTNNAC 1 cut(s) 55
Hpy188I TCNGA 6 cut(s) 21, 235, 412, 470, 518, 613
Hpy188III TCNNGA 6 cut(s) 149, 301, 391, 509, 679, 804
Hpy8I GTNNAC 1 cut(s) 55
Hpy99I CGWCG 1 cut(s) 527
HpyCH4III ACNGT 2 cut(s) 90, 476
HpyCH4V TGCA 5 cut(s) 186, 275, 336, 405, 578
HpyF10VI GCNNNNNNNGC 1 cut(s) 631
HpyF3I CTNAG 2 cut(s) 399, 779
Hsp92II CATG 6 cut(s) 50, 115, 194, 336, 394, 790
HspAI GCGC 1 cut(s) 625
Ksp22I TGATCA 1 cut(s) 297
Kzo9I GATC 4 cut(s) 227, 297, 501, 597
LguI GCTCTTC 1 cut(s) 534
Lsp1109I GCAGC 2 cut(s) 181, 562
LweI GCATC 2 cut(s) 154, 660
MaeI CTAG 4 cut(s) 288, 381, 455, 656
MaeIII GTNAC 1 cut(s) 90
MalI GATC 4 cut(s) 229, 299, 503, 599
MboI GATC 4 cut(s) 227, 297, 501, 597
MboII GAAGA 4 cut(s) 76, 254, 521, 626
MfeI CAATTG 1 cut(s) 664
MluCI AATT 8 cut(s) 154, 307, 326, 370, 406, 512, 587, 664
MnlI CCTC 7 cut(s) 15, 130, 133, 444, 503, 610, 613
Mph1103I ATGCAT 1 cut(s) 188
MseI TTAA 1 cut(s) 717
MspCI CTTAAG 1 cut(s) 716
MspI CCGG 1 cut(s) 450
MspR9I CCNGG 1 cut(s) 438
MunI CAATTG 1 cut(s) 664
MvaI CCWGG 1 cut(s) 438
MwoI GCNNNNNNNGC 1 cut(s) 631
NdeII GATC 4 cut(s) 227, 297, 501, 597
NlaIII CATG 6 cut(s) 50, 115, 194, 336, 394, 790
NlaIV GGNNCC 2 cut(s) 351, 776
NmuCI GTSAC 1 cut(s) 90
NsbI TGCGCA 1 cut(s) 626
NsiI ATGCAT 1 cut(s) 188
NspI RCATGY 2 cut(s) 50, 336
PagI TCATGA 1 cut(s) 390
PciI ACATGT 1 cut(s) 46
PciSI GCTCTTC 1 cut(s) 534
PkrI GCNGC 3 cut(s) 171, 174, 577
PscI ACATGT 1 cut(s) 46
PsiI TTATAA 1 cut(s) 375
Psp6I CCWGG 1 cut(s) 436
PspGI CCWGG 1 cut(s) 436
PspN4I GGNNCC 2 cut(s) 351, 776
RsaI GTAC 1 cut(s) 746
RsaNI GTAC 1 cut(s) 745
SapI GCTCTTC 1 cut(s) 534
SaqAI TTAA 1 cut(s) 717
SatI GCNGC 3 cut(s) 170, 173, 576
Sau3AI GATC 4 cut(s) 227, 297, 501, 597
ScaI AGTACT 1 cut(s) 746
ScrFI CCNGG 1 cut(s) 438
SetI ASST 9 cut(s) 26, 41, 165, 293, 624, 661, 694, 764, 780
SfaNI GCATC 2 cut(s) 154, 660
SfcI CTRYAG 1 cut(s) 702
SmlI CTYRAG 2 cut(s) 95, 716
SmoI CTYRAG 2 cut(s) 95, 716
Sse9I AATT 8 cut(s) 154, 307, 326, 370, 406, 512, 587, 664
SsiI CCGC 1 cut(s) 173
SspMI CTAG 4 cut(s) 288, 381, 455, 656
StyD4I CCNGG 1 cut(s) 436
StyI CCWWGG 3 cut(s) 58, 159, 287
TaaI ACNGT 2 cut(s) 90, 476
TaqI TCGA 2 cut(s) 421, 495
TasI AATT 8 cut(s) 154, 307, 326, 370, 406, 512, 587, 664
TatI WGTACW 1 cut(s) 744
TauI GCSGC 1 cut(s) 175
Tru1I TTAA 1 cut(s) 717
Tru9I TTAA 1 cut(s) 717
TscAI CASTG 4 cut(s) 448, 481, 740, 776
TseFI GTSAC 1 cut(s) 90
TseI GCWGC 2 cut(s) 169, 575
Tsp45I GTSAC 1 cut(s) 90
TspDTI ATGAA 7 cut(s) 222, 320, 379, 419, 561, 659, 742
TspGWI ACGGA 1 cut(s) 342
TspRI CASTG 4 cut(s) 448, 481, 740, 776
Vha464I CTTAAG 1 cut(s) 716
XapI RAATTY 2 cut(s) 154, 512
XceI RCATGY 2 cut(s) 50, 336
XmaJI CCTAGG 1 cut(s) 287
XspI CTAG 4 cut(s) 288, 381, 455, 656
ZrmI AGTACT 1 cut(s) 746
Zsp2I ATGCAT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.