Rmu_ssc0000029.1_g000004

Vacuolar amino acid transporter 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000029.1
OG0009601
Physical Location & Seq
Forward (+)
25529 .. 29341
3813 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2367 bp
atggagttggactccctcaaagtagagaagatagagagccaaattcaaccacttgaaccaaacaaaggcaccacctttttcagaacctgtttcaatggactcaacacactgtcaggtgtgggaatactatcagttccatttgctctatctcagggagggtggttgagcttagtgcttctcttctcggtagcaatcctatgttggtatacagggctgcttctacagcgatgtatggactcaaattcgcacataaaaacttatcccgacattggtcaagctgcttttggacgcaaaggaagagtggccatatccatctttatgtatcttgagctgtatttagttgcagttgaatttctaatactagaaggtgataacttaaaccagttgtttccaaacgtgagttttaacatgggaggccacacaattggaggtaagcaatgctttgttttagccactgcccttgtaattctgccgactacatggttgaagagcttgggattgctggcatatatttcagctggaggggttttggcttcgattattttggttgcttgtgttttttgggttggtgctgttgatagtgtgggatttcatgaaagaggtgatctcctgaattggaaaggattgcctactgctataagcttgtatttgttctgttactgtggtcatgcagtttttcccacattatgcaattccatgaaagatagaagccaattccccaaggtattgcttgtttgcttcataacaagcaccatcacctatggatccatggccgttttgggttacttgatgtttggagaatatttgaagtcccaagtgacattaaatcttcctgtacaaaaaattagtgctaaagtagcaattttcatcacaatagttaaccccttgactaagtatgcaattataatcactccaattgctactgctatagaggactcacgctatttttgcaacagtcatgctattatcagcatcatggtgaggacggggattgtgatcagcaccgtatgtgtggcattgatggttccatttttcggctatgtcatggcattcgtcggtgcttttctgagtgtaactgtctcaatattgttgccgtgcctgttctacctgaagattaacaatgttgctctcaggtttgggttcgagttgatgacaattgttgggattatggtgatgggatcttttgttggcgtagtaggcacttacacttctctgaaagaaattgggaaaggtcgcagcctatccggcggcgccccggcgtcgattgtggttccttgctgcattgacgagtggtgggtgttgccggatgggttatcggggcgaccagtggccggatcattgcggaggcactccatggattgtaaggcgctgggtgcttctctgtggtgggatgctaggaggcgattgcagtggtggtctcgagttcttaagctgaaggacgtagactcttggagttctctctctccgaggtggcaaggcaccatgttctggcttgctgcgtggtttgggtttctccggcgaggtgagctcgaaggggtaaccttgtggtggtggtggcctgcaggcgaggctcctggggagaaggatgaggattgtggcagctgtattttatttttgtttttgcaagttatgtttttggttaggttttattggtcactagcatgtccctactccgttgagctagggttgggtcaatttgaggtgccatggatgtggtgtcattcctggggacgaattggtttaatttcggtgttatcttatggtcagtgtgctgacgagcgatccttgcacgtggtctggttgtgggacatggtgtggaagagggcgttggtttttctggctgttgtccatgaggtggtatcaagatactcgggattccttgtagcccgagagtttgggcggtataggtggttaagggttgggccctttcggctcatggaggtcattcatgatgacggacccgtaactggtttaggtttttgggaggagagtggggagttcatgtcccccactggtcattcccgtttgattcaaaagtcaaaacgagcagaatcaaacattttcagtactagcattgatgatctccccgaaactgcattggtggaaatcctttgtcgcttgcggagttgcaaatatgttgttcaatgcaagtgtgtttcgaaacgttggtgcaatcttttgtcggatccttatttgattggtcgctttctgtggctccaattgggtcagatacccccaattgtaactcgtagtactcccatagtaaacagcaatttcgggcttgaactcttcactgctgaggtgtttgtgaaactaaggaatttgttcagtttgaaaagtaatccactggtgcttggaatgacttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04721 ko04723 ko04727 ko05032 ko05033 map04721 map04723 map04727 map05032 map05033
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

788

Amino Acids

88.21

Weight (kDa)

8.71

Isoelectric Point (pI)

38.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 23 - 402 6.9e-66 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 905
AccB1I GGYRCC 4 cut(s) 68, 1250, 1478, 1702
AccB7I CCANNNNNTGG 2 cut(s) 1488, 1855
AccI GTMKAC 2 cut(s) 206, 1443
AciI CCGC 4 cut(s) 1248, 1342, 1900, 2122
AclI AACGTT 1 cut(s) 2164
AclWI GGATC 7 cut(s) 759, 772, 1186, 1342, 1776, 2180, 2193
AcoI YGGCCR 3 cut(s) 303, 771, 1329
AcsI RAATTY 4 cut(s) 42, 241, 350, 2320
AcuI CTGAAG 2 cut(s) 1130, 1454
AcvI CACGTG 1 cut(s) 1792
AcyI GRCGYC 2 cut(s) 1251, 1259
AfaI GTAC 3 cut(s) 837, 2068, 2254
AfiI CCNNNNNNNGG 8 cut(s) 65, 269, 1034, 1331, 1488, 1548, 1855, 1967
AflII CTTAAG 1 cut(s) 1427
AjnI CCWGG 2 cut(s) 1573, 1724
AjuI GAANNNNNNNTTGG 2 cut(s) 1811, 1843
Alw21I GWGCWC 1 cut(s) 1530
Alw26I GTCTC 2 cut(s) 1084, 1422
AlwI GGATC 7 cut(s) 759, 772, 1186, 1342, 1776, 2180, 2193
AlwNI CAGNNNCTG 1 cut(s) 87
Ama87I CYCGRG 3 cut(s) 1419, 1870, 1887
AoxI GGCC 6 cut(s) 303, 415, 771, 1329, 1556, 1922
ApaI GGGCCC 1 cut(s) 1926
ApeKI GCWGC 6 cut(s) 214, 278, 1236, 1278, 1496, 1599
ApoI RAATTY 4 cut(s) 42, 241, 350, 2320
Asp700I GAANNNNTTC 1 cut(s) 2324
AspLEI GCGC 2 cut(s) 1253, 1369
AspS9I GGNCC 3 cut(s) 1922, 1923, 1958
AsuC2I CCSGG 1 cut(s) 1256
AsuHPI GGTGA 6 cut(s) 380, 614, 748, 991, 1183, 1535
AsuII TTCGAA 1 cut(s) 2159
AvaI CYCGRG 3 cut(s) 1419, 1870, 1887
AvaII GGWCC 1 cut(s) 1958
BaeGI GKGCMC 1 cut(s) 1926
BalI TGGCCA 1 cut(s) 305
BamHI GGATCC 2 cut(s) 764, 2185
BanI GGYRCC 4 cut(s) 68, 1250, 1478, 1702
BanII GRGCYC 2 cut(s) 1530, 1926
BbrPI CACGTG 1 cut(s) 1792
Bbv12I GWGCWC 1 cut(s) 1530
BbvCI CCTCAGC 1 cut(s) 2298
BbvI GCAGC 6 cut(s) 201, 265, 1248, 1265, 1483, 1611
BccI CCATC 5 cut(s) 320, 761, 1015, 1168, 1301
BceAI ACGGC 2 cut(s) 758, 1078
BciT130I CCWGG 2 cut(s) 1575, 1726
BclI TGATCA 1 cut(s) 996
BcnI CCSGG 1 cut(s) 1256
BcoDI GTCTC 2 cut(s) 1084, 1422
BfaI CTAG 5 cut(s) 362, 1395, 1658, 1682, 2070
BfmI CTRYAG 3 cut(s) 221, 927, 1560
BfoI RGCGCY 2 cut(s) 1254, 1370
BfrI CTTAAG 1 cut(s) 1427
BglI GCCNNNNNGGC 2 cut(s) 1245, 1930
BisI GCNGC 7 cut(s) 215, 279, 1237, 1249, 1279, 1497, 1600
BlsI GCNGC 7 cut(s) 216, 280, 1238, 1250, 1280, 1498, 1601
BmcAI AGTACT 2 cut(s) 2068, 2254
Bme1390I CCNGG 3 cut(s) 1256, 1575, 1726
Bme18I GGWCC 1 cut(s) 1958
BmeT110I CYCGRG 3 cut(s) 1419, 1870, 1887
BmgT120I GGNCC 3 cut(s) 1922, 1923, 1958
BmrFI CCNGG 3 cut(s) 1256, 1575, 1726
BmsI GCATC 2 cut(s) 981, 1381
BoxI GACNNNNGTC 1 cut(s) 270
BplI GAGNNNNNCTC 5 cut(s) 28, 591, 623, 1512, 1544
BpmI CTGGAG 1 cut(s) 540
Bpu10I CCTNAGC 1 cut(s) 2298
Bpu14I TTCGAA 1 cut(s) 2159
BpuEI CTTGAG 1 cut(s) 347
BpuMI CCSGG 1 cut(s) 1256
BsaAI YACGTR 1 cut(s) 1792
BsaBI GATNNNNATC 1 cut(s) 995
BsaHI GRCGYC 2 cut(s) 1251, 1259
BsaI GGTCTC 1 cut(s) 1422
BsaJI CCNNGG 8 cut(s) 720, 768, 1254, 1353, 1466, 1574, 1706, 1725
BsaXI ACNNNNNCTCC 2 cut(s) 1988, 2018
Bsc4I CCNNNNNNNGG 8 cut(s) 65, 269, 1034, 1331, 1488, 1548, 1855, 1967
Bse1I ACTGG 5 cut(s) 382, 1325, 1972, 2017, 2352
Bse3DI GCAATG 2 cut(s) 443, 1337
Bse8I GATNNNNATC 1 cut(s) 995
BseBI CCWGG 2 cut(s) 1575, 1726
BseDI CCNNGG 8 cut(s) 720, 768, 1254, 1353, 1466, 1574, 1706, 1725
BseGI GGATG 4 cut(s) 1312, 1396, 1591, 1716
BseJI GATNNNNATC 1 cut(s) 995
BseLI CCNNNNNNNGG 8 cut(s) 65, 269, 1034, 1331, 1488, 1548, 1855, 1967
BseMI GCAATG 2 cut(s) 443, 1337
BseMII CTCAG 4 cut(s) 164, 1058, 1144, 2289
BseNI ACTGG 5 cut(s) 382, 1325, 1972, 2017, 2352
BseRI GAGGAG 1 cut(s) 2000
BseSI GKGCMC 1 cut(s) 1926
BseXI GCAGC 6 cut(s) 201, 265, 1248, 1265, 1483, 1611
BseYI CCCAGC 1 cut(s) 1369
BshFI GGCC 6 cut(s) 305, 417, 773, 1331, 1558, 1924
BshNI GGYRCC 4 cut(s) 68, 1250, 1478, 1702
BsiHKAI GWGCWC 1 cut(s) 1530
BsiHKCI CYCGRG 3 cut(s) 1419, 1870, 1887
BsiSI CCGG 5 cut(s) 1245, 1256, 1304, 1332, 1516
BslFI GGGAC 5 cut(s) 796, 1650, 1743, 1820, 1990
BslI CCNNNNNNNGG 8 cut(s) 65, 269, 1034, 1331, 1488, 1548, 1855, 1967
BsmAI GTCTC 2 cut(s) 1084, 1422
BsmFI GGGAC 5 cut(s) 796, 1650, 1743, 1820, 1990
BsmI GAATGC 1 cut(s) 1049
BsnI GGCC 6 cut(s) 305, 417, 773, 1331, 1558, 1924
Bso31I GGTCTC 1 cut(s) 1422
BsoBI CYCGRG 3 cut(s) 1419, 1870, 1887
Bsp119I TTCGAA 1 cut(s) 2159
Bsp120I GGGCCC 1 cut(s) 1922
Bsp1286I GDGCHC 2 cut(s) 1530, 1926
Bsp1407I TGTACA 1 cut(s) 835
Bsp143I GATC 8 cut(s) 604, 764, 996, 1178, 1334, 1781, 2080, 2185
Bsp19I CCATGG 3 cut(s) 768, 1353, 1706
BspACI CCGC 4 cut(s) 1248, 1342, 1900, 2122
BspANI GGCC 6 cut(s) 305, 417, 773, 1331, 1558, 1924
BspCNI CTCAG 4 cut(s) 163, 1059, 1143, 2290
BspHI TCATGA 2 cut(s) 592, 1948
BspMAI CTGCAG 1 cut(s) 1564
BspPI GGATC 7 cut(s) 759, 772, 1186, 1342, 1776, 2180, 2193
BspQI GCTCTTC 1 cut(s) 482
BspT104I TTCGAA 1 cut(s) 2159
BspT107I GGYRCC 4 cut(s) 68, 1250, 1478, 1702
BspTI CTTAAG 1 cut(s) 1427
BspTNI GGTCTC 1 cut(s) 1422
BsrDI GCAATG 2 cut(s) 443, 1337
BsrGI TGTACA 1 cut(s) 835
BsrI ACTGG 5 cut(s) 382, 1325, 1972, 2017, 2352
BssECI CCNNGG 8 cut(s) 720, 768, 1254, 1353, 1466, 1574, 1706, 1725
BssMI GATC 8 cut(s) 604, 764, 996, 1178, 1334, 1781, 2080, 2185
BssNAI GTATAC 1 cut(s) 207
BssNI GRCGYC 2 cut(s) 1251, 1259
BssT1I CCWWGG 4 cut(s) 720, 768, 1353, 1706
Bst1107I GTATAC 1 cut(s) 207
Bst2UI CCWGG 2 cut(s) 1575, 1726
Bst4CI ACNGT 5 cut(s) 111, 662, 956, 1006, 1078
Bst6I CTCTTC 5 cut(s) 185, 292, 482, 1814, 2294
BstACI GRCGYC 2 cut(s) 1251, 1259
BstAFI CTTAAG 1 cut(s) 1427
BstAUI TGTACA 1 cut(s) 835
BstBAI YACGTR 1 cut(s) 1792
BstBI TTCGAA 1 cut(s) 2159
BstC8I GCNNGC 5 cut(s) 504, 1494, 1560, 1564, 2120
BstDEI CTNAG 8 cut(s) 150, 169, 891, 1067, 1130, 2298, 2315, 2364
BstDSI CCRYGG 3 cut(s) 768, 1353, 1706
BstEII GGTNACC 1 cut(s) 1537
BstF5I GGATG 4 cut(s) 1312, 1396, 1591, 1716
BstH2I RGCGCY 2 cut(s) 1254, 1370
BstHHI GCGC 2 cut(s) 1253, 1369
BstKTI GATC 8 cut(s) 607, 767, 999, 1181, 1337, 1784, 2083, 2188
BstMAI GTCTC 2 cut(s) 1084, 1422
BstMBI GATC 8 cut(s) 604, 764, 996, 1178, 1334, 1781, 2080, 2185
BstNI CCWGG 2 cut(s) 1575, 1726
BstNSI RCATGY 1 cut(s) 1665
BstPAI GACNNNNGTC 1 cut(s) 270
BstPI GGTNACC 1 cut(s) 1537
BstSCI CCNGG 3 cut(s) 1254, 1573, 1724
BstSFI CTRYAG 3 cut(s) 221, 927, 1560
BstSLI GKGCMC 1 cut(s) 1926
BstV1I GCAGC 6 cut(s) 201, 265, 1248, 1265, 1483, 1611
BstX2I RGATCY 3 cut(s) 764, 1178, 2185
BstXI CCANNNNNNTGG 2 cut(s) 425, 1713
BstYI RGATCY 3 cut(s) 764, 1178, 2185
BstZ17I GTATAC 1 cut(s) 207
BsuRI GGCC 6 cut(s) 305, 417, 773, 1331, 1558, 1924
BtgI CCRYGG 3 cut(s) 768, 1353, 1706
BtgZI GCGATG 1 cut(s) 241
BtsCI GGATG 4 cut(s) 1312, 1396, 1591, 1716
BtsI GCAGTG 3 cut(s) 453, 1415, 2292
BtsIMutI CAGTG 8 cut(s) 107, 453, 1332, 1415, 1772, 2010, 2292, 2345
Cac8I GCNNGC 5 cut(s) 504, 1494, 1560, 1564, 2120
CaiI CAGNNNCTG 1 cut(s) 87
CciI TCATGA 2 cut(s) 592, 1948
CfoI GCGC 2 cut(s) 1253, 1369
Cfr13I GGNCC 3 cut(s) 1922, 1923, 1958
CseI GACGC 2 cut(s) 297, 1248
Csp6I GTAC 3 cut(s) 836, 2067, 2253
CspCI CAANNNNNGTGG 2 cut(s) 442, 477
CviQI GTAC 3 cut(s) 836, 2067, 2253
DdeI CTNAG 8 cut(s) 150, 169, 891, 1067, 1130, 2298, 2315, 2364
DinI GGCGCC 1 cut(s) 1252
DpnI GATC 8 cut(s) 606, 766, 998, 1180, 1336, 1783, 2082, 2187
DpnII GATC 8 cut(s) 604, 764, 996, 1178, 1334, 1781, 2080, 2185
EaeI YGGCCR 3 cut(s) 303, 771, 1329
Eam1104I CTCTTC 5 cut(s) 185, 292, 482, 1814, 2294
EarI CTCTTC 5 cut(s) 185, 292, 482, 1814, 2294
Ecl136II GAGCTC 1 cut(s) 1528
Eco130I CCWWGG 4 cut(s) 720, 768, 1353, 1706
Eco24I GRGCYC 2 cut(s) 1530, 1926
Eco31I GGTCTC 1 cut(s) 1422
Eco47I GGWCC 1 cut(s) 1958
Eco53kI GAGCTC 1 cut(s) 1528
Eco57I CTGAAG 2 cut(s) 1130, 1454
Eco72I CACGTG 1 cut(s) 1792
Eco88I CYCGRG 3 cut(s) 1419, 1870, 1887
Eco91I GGTNACC 1 cut(s) 1537
EcoICRI GAGCTC 1 cut(s) 1528
EcoO109I RGGNCCY 1 cut(s) 1923
EcoO65I GGTNACC 1 cut(s) 1537
EcoRII CCWGG 2 cut(s) 1573, 1724
EcoT14I CCWWGG 4 cut(s) 720, 768, 1353, 1706
EcoT38I GRGCYC 2 cut(s) 1530, 1926
EgeI GGCGCC 1 cut(s) 1252
EheI GGCGCC 1 cut(s) 1252
ErhI CCWWGG 4 cut(s) 720, 768, 1353, 1706
FalI AAGNNNNNCTT 2 cut(s) 425, 457
FaqI GGGAC 5 cut(s) 796, 1650, 1743, 1820, 1990
FbaI TGATCA 1 cut(s) 996
FblI GTMKAC 2 cut(s) 206, 1443
Fnu4HI GCNGC 7 cut(s) 215, 279, 1237, 1249, 1279, 1497, 1600
FokI GGATG 4 cut(s) 1319, 1403, 1598, 1723
FriOI GRGCYC 2 cut(s) 1530, 1926
Fsp4HI GCNGC 7 cut(s) 215, 279, 1237, 1249, 1279, 1497, 1600
FspBI CTAG 5 cut(s) 362, 1395, 1658, 1682, 2070
GlaI GCGC 2 cut(s) 1252, 1368
GluI GCNGC 7 cut(s) 215, 279, 1237, 1249, 1279, 1497, 1600
GsaI CCCAGC 1 cut(s) 1373
GsuI CTGGAG 1 cut(s) 540
HaeII RGCGCY 2 cut(s) 1254, 1370
HaeIII GGCC 6 cut(s) 305, 417, 773, 1331, 1558, 1924
HapII CCGG 5 cut(s) 1245, 1256, 1304, 1332, 1516
HgaI GACGC 2 cut(s) 297, 1248
HhaI GCGC 2 cut(s) 1253, 1369
Hin1I GRCGYC 2 cut(s) 1251, 1259
Hin6I GCGC 2 cut(s) 1251, 1367
HinP1I GCGC 2 cut(s) 1251, 1367
HincII GTYRAC 1 cut(s) 880
HindII GTYRAC 1 cut(s) 880
HindIII AAGCTT 1 cut(s) 640
HinfI GANTC 8 cut(s) 11, 99, 236, 935, 1445, 1875, 2029, 2051
HpaI GTTAAC 1 cut(s) 880
HpaII CCGG 5 cut(s) 1245, 1256, 1304, 1332, 1516
HphI GGTGA 6 cut(s) 380, 614, 748, 991, 1183, 1535
Hpy166II GTNNAC 4 cut(s) 207, 880, 1444, 2266
Hpy188I TCNGA 6 cut(s) 83, 1068, 1215, 1467, 2185, 2229
Hpy188III TCNNGA 8 cut(s) 263, 326, 593, 610, 1419, 1863, 1872, 1949
Hpy8I GTNNAC 4 cut(s) 207, 880, 1444, 2266
Hpy99I CGWCG 2 cut(s) 1058, 1264
HpyAV CCTTC 4 cut(s) 359, 1429, 1526, 1576
HpyCH4III ACNGT 5 cut(s) 111, 662, 956, 1006, 1078
HpyCH4IV ACGT 4 cut(s) 396, 1440, 1791, 2164
HpyF3I CTNAG 8 cut(s) 150, 169, 891, 1067, 1130, 2298, 2315, 2364
HpySE526I ACGT 4 cut(s) 396, 1440, 1791, 2164
Hsp92I GRCGYC 2 cut(s) 1251, 1259
HspAI GCGC 2 cut(s) 1251, 1367
KasI GGCGCC 1 cut(s) 1250
Ksp22I TGATCA 1 cut(s) 996
KspAI GTTAAC 1 cut(s) 880
Kzo9I GATC 8 cut(s) 604, 764, 996, 1178, 1334, 1781, 2080, 2185
LguI GCTCTTC 1 cut(s) 482
LmnI GCTCC 2 cut(s) 1576, 2220
Lsp1109I GCAGC 6 cut(s) 201, 265, 1248, 1265, 1483, 1611
LweI GCATC 2 cut(s) 981, 1381
MaeI CTAG 5 cut(s) 362, 1395, 1658, 1682, 2070
MaeII ACGT 4 cut(s) 396, 1440, 1791, 2164
MaeIII GTNAC 8 cut(s) 656, 782, 817, 1072, 1537, 1653, 1963, 2242
MalI GATC 8 cut(s) 606, 766, 998, 1180, 1336, 1783, 2082, 2187
MboI GATC 8 cut(s) 604, 764, 996, 1178, 1334, 1781, 2080, 2185
MboII GAAGA 8 cut(s) 40, 172, 309, 499, 821, 1123, 1831, 2281
MfeI CAATTG 5 cut(s) 423, 915, 1155, 2219, 2238
MflI RGATCY 3 cut(s) 764, 1178, 2185
MhlI GDGCHC 2 cut(s) 1530, 1926
MlsI TGGCCA 1 cut(s) 305
MluNI TGGCCA 1 cut(s) 305
Mly113I GGCGCC 1 cut(s) 1251
MlyI GAGTC 5 cut(s) 5, 93, 230, 929, 1439
MmeI TCCRAC 1 cut(s) 2163
Mox20I TGGCCA 1 cut(s) 305
MroXI GAANNNNTTC 1 cut(s) 2324
MscI TGGCCA 1 cut(s) 305
MseI TTAA 8 cut(s) 377, 405, 824, 879, 1116, 1428, 1742, 1913
MslI CAYNNNNRTG 4 cut(s) 317, 977, 1660, 1711
Msp20I TGGCCA 1 cut(s) 305
MspA1I CMGCKG 2 cut(s) 518, 1602
MspCI CTTAAG 1 cut(s) 1427
MspI CCGG 5 cut(s) 1245, 1256, 1304, 1332, 1516
MspR9I CCNGG 3 cut(s) 1256, 1575, 1726
MunI CAATTG 5 cut(s) 423, 915, 1155, 2219, 2238
Mva1269I GAATGC 1 cut(s) 1049
MvaI CCWGG 2 cut(s) 1575, 1726
NarI GGCGCC 1 cut(s) 1251
NciI CCSGG 1 cut(s) 1256
NcoI CCATGG 3 cut(s) 768, 1353, 1706
NdeII GATC 8 cut(s) 604, 764, 996, 1178, 1334, 1781, 2080, 2185
NmuCI GTSAC 2 cut(s) 817, 1653
NspI RCATGY 1 cut(s) 1665
NspV TTCGAA 1 cut(s) 2159
PaeR7I CTCGAG 1 cut(s) 1419
PagI TCATGA 2 cut(s) 592, 1948
PciSI GCTCTTC 1 cut(s) 482
PctI GAATGC 1 cut(s) 1049
PdmI GAANNNNTTC 1 cut(s) 2324
PfeI GAWTC 3 cut(s) 1875, 2029, 2051
PflMI CCANNNNNTGG 2 cut(s) 1488, 1855
PkrI GCNGC 7 cut(s) 216, 280, 1238, 1250, 1280, 1498, 1601
PleI GAGTC 5 cut(s) 5, 93, 230, 929, 1439
PluTI GGCGCC 1 cut(s) 1254
PmaCI CACGTG 1 cut(s) 1792
PmlI CACGTG 1 cut(s) 1792
PpsI GAGTC 5 cut(s) 5, 93, 230, 929, 1439
Ppu21I YACGTR 1 cut(s) 1792
PshAI GACNNNNGTC 1 cut(s) 270
PsiI TTATAA 1 cut(s) 905
Psp124BI GAGCTC 1 cut(s) 1530
Psp1406I AACGTT 1 cut(s) 2164
Psp6I CCWGG 2 cut(s) 1573, 1724
PspCI CACGTG 1 cut(s) 1792
PspEI GGTNACC 1 cut(s) 1537
PspFI CCCAGC 1 cut(s) 1369
PspGI CCWGG 2 cut(s) 1573, 1724
PspOMI GGGCCC 1 cut(s) 1922
PspPI GGNCC 3 cut(s) 1922, 1923, 1958
PstI CTGCAG 1 cut(s) 1564
PstNI CAGNNNCTG 1 cut(s) 87
PsuI RGATCY 3 cut(s) 764, 1178, 2185
PvuII CAGCTG 2 cut(s) 518, 1602
RsaI GTAC 3 cut(s) 837, 2068, 2254
RsaNI GTAC 3 cut(s) 836, 2067, 2253
RseI CAYNNNNRTG 4 cut(s) 317, 977, 1660, 1711
SacI GAGCTC 1 cut(s) 1530
SapI GCTCTTC 1 cut(s) 482
SaqAI TTAA 8 cut(s) 377, 405, 824, 879, 1116, 1428, 1742, 1913
SatI GCNGC 7 cut(s) 215, 279, 1237, 1249, 1279, 1497, 1600
Sau3AI GATC 8 cut(s) 604, 764, 996, 1178, 1334, 1781, 2080, 2185
Sau96I GGNCC 3 cut(s) 1922, 1923, 1958
SbfI CCTGCAGG 1 cut(s) 1564
ScaI AGTACT 2 cut(s) 2068, 2254
SchI GAGTC 5 cut(s) 5, 93, 230, 929, 1439
ScrFI CCNGG 3 cut(s) 1256, 1575, 1726
SdaI CCTGCAGG 1 cut(s) 1564
SduI GDGCHC 2 cut(s) 1530, 1926
SfaNI GCATC 2 cut(s) 981, 1381
SfcI CTRYAG 3 cut(s) 221, 927, 1560
SfoI GGCGCC 1 cut(s) 1252
Sfr274I CTCGAG 1 cut(s) 1419
SfuI TTCGAA 1 cut(s) 2159
SinI GGWCC 1 cut(s) 1958
SlaI CTCGAG 1 cut(s) 1419
SmiMI CAYNNNNRTG 4 cut(s) 317, 977, 1660, 1711
SmlI CTYRAG 3 cut(s) 326, 1419, 1427
SmoI CTYRAG 3 cut(s) 326, 1419, 1427
Sse8387I CCTGCAGG 1 cut(s) 1564
SsiI CCGC 4 cut(s) 1248, 1342, 1900, 2122
SspDI GGCGCC 1 cut(s) 1250
SspI AATATT 2 cut(s) 803, 1086
SspMI CTAG 5 cut(s) 362, 1395, 1658, 1682, 2070
SstI GAGCTC 1 cut(s) 1530
StyD4I CCNGG 3 cut(s) 1254, 1573, 1724
StyI CCWWGG 4 cut(s) 720, 768, 1353, 1706
TaaI ACNGT 5 cut(s) 111, 662, 956, 1006, 1078
TaiI ACGT 4 cut(s) 399, 1443, 1794, 2167
TaqI TCGA 6 cut(s) 536, 1143, 1262, 1420, 1530, 2159
TatI WGTACW 3 cut(s) 835, 2066, 2252
TauI GCSGC 1 cut(s) 1251
TfiI GAWTC 3 cut(s) 1875, 2029, 2051
Tru1I TTAA 8 cut(s) 377, 405, 824, 879, 1116, 1428, 1742, 1913
Tru9I TTAA 8 cut(s) 377, 405, 824, 879, 1116, 1428, 1742, 1913
TscAI CASTG 8 cut(s) 114, 460, 1332, 1415, 1772, 2017, 2299, 2352
TseFI GTSAC 2 cut(s) 817, 1653
TseI GCWGC 6 cut(s) 214, 278, 1236, 1278, 1496, 1599
Tsp45I GTSAC 2 cut(s) 817, 1653
TspDTI ATGAA 7 cut(s) 581, 609, 713, 730, 856, 1937, 1990
TspGWI ACGGA 2 cut(s) 1663, 1971
TspRI CASTG 8 cut(s) 114, 460, 1332, 1415, 1772, 2017, 2299, 2352
Van91I CCANNNNNTGG 2 cut(s) 1488, 1855
Vha464I CTTAAG 1 cut(s) 1427
VpaK11BI GGWCC 1 cut(s) 1958
XapI RAATTY 4 cut(s) 42, 241, 350, 2320
XceI RCATGY 1 cut(s) 1665
XcmI CCANNNNNNNNNTGG 1 cut(s) 775
XhoI CTCGAG 1 cut(s) 1419
XmiI GTMKAC 2 cut(s) 206, 1443
XmnI GAANNNNTTC 1 cut(s) 2324
XspI CTAG 5 cut(s) 362, 1395, 1658, 1682, 2070
ZrmI AGTACT 2 cut(s) 2068, 2254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.