Rmu_ssc0000110.1_g000001

Mediator-associated protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000110.1
OG0008960
Physical Location & Seq
Forward (+)
1 .. 645
645 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 645 bp
atgggcgcgttccatgagttcttgaagagaagcttgaaggctgacgtcagcaagaaccagttgatggataagaccaggaggttgaagaagaagtaccatacgaatgctgagaaaggcgagaatggggagggcccggtgttttcgaaggcgcatgagttgaagtcgtttgatttgtcgaagaagatttggggagctgaggctaatgggggagatgatagtgcaaagaacagtaggaagaagccgaggaagagtgccaaggctaacaacaacaacaactcttctacagtcttggcattgcctgtttcggatgctgcggctgagaggagtgtggagaagaaagtaaaagtggaggagatgaatgttgctaatggcggagttaaggctgctgagtccgatgacttttggtccaagtacccatcgctgagtgattctttgcagcttgcaaattgctcatcgaggtgggagggtttggagagagttatgattcagaaaatgccttcaattgggagctcaaaggctaaagagttggatgataggtggaggaagttgcagctcttagagatggagttgtctgtcaagaagcttgatttgatgcatgaacaggctaagcttttattggatgggataaagtcgcggaaggattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF573

Protein Analysis

214

Amino Acids

23.96

Weight (kDa)

9.59

Isoelectric Point (pI)

37.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GeBP-like_DBD PF04504 1 - 64 2.2e-19 Glabrous-enhancer-binding protein-like family, DBD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 48
AccB7I CCANNNNNTGG 1 cut(s) 64
AccII CGCG 2 cut(s) 8, 634
AciI CCGC 3 cut(s) 314, 372, 634
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 2 cut(s) 95, 413
AfiI CCNNNNNNNGG 2 cut(s) 64, 503
AgsI TTSAA 5 cut(s) 25, 37, 85, 160, 501
AhdI GACNNNNNGTC 1 cut(s) 403
AjnI CCWGG 1 cut(s) 74
AluBI AGCT 7 cut(s) 33, 194, 439, 510, 553, 583, 610
AluI AGCT 7 cut(s) 33, 194, 439, 510, 553, 583, 610
Alw21I GWGCWC 1 cut(s) 512
AoxI GGCC 1 cut(s) 130
ApaI GGGCCC 1 cut(s) 134
ApeKI GCWGC 4 cut(s) 311, 383, 436, 550
AspLEI GCGC 2 cut(s) 8, 151
AspS9I GGNCC 3 cut(s) 130, 131, 405
AsuC2I CCSGG 1 cut(s) 134
AsuII TTCGAA 1 cut(s) 143
AvaII GGWCC 1 cut(s) 405
BaeGI GKGCMC 1 cut(s) 134
BanII GRGCYC 2 cut(s) 134, 512
BarI GAAGNNNNNNTAC 2 cut(s) 77, 109
Bbv12I GWGCWC 1 cut(s) 512
BbvCI CCTCAGC 1 cut(s) 195
BbvI GCAGC 4 cut(s) 298, 370, 448, 562
BccI CCATC 4 cut(s) 58, 424, 556, 614
BciT130I CCWGG 1 cut(s) 76
BcnI CCSGG 1 cut(s) 134
BfmI CTRYAG 1 cut(s) 282
BisI GCNGC 5 cut(s) 312, 315, 384, 437, 551
BlpI GCTNAGC 1 cut(s) 606
BlsI GCNGC 5 cut(s) 313, 316, 385, 438, 552
Bme1390I CCNGG 2 cut(s) 76, 134
Bme18I GGWCC 1 cut(s) 405
BmeRI GACNNNNNGTC 1 cut(s) 403
BmgT120I GGNCC 3 cut(s) 130, 131, 405
BmiI GGNNCC 1 cut(s) 132
BmrFI CCNGG 2 cut(s) 76, 134
BmsI GCATC 2 cut(s) 298, 582
Bpu10I CCTNAGC 1 cut(s) 195
Bpu1102I GCTNAGC 1 cut(s) 606
Bpu14I TTCGAA 1 cut(s) 143
BpuMI CCSGG 1 cut(s) 134
BsaHI GRCGYC 1 cut(s) 45
BsaJI CCNNGG 2 cut(s) 242, 255
Bsc4I CCNNNNNNNGG 2 cut(s) 64, 503
Bse1I ACTGG 1 cut(s) 58
Bse3DI GCAATG 1 cut(s) 293
BseBI CCWGG 1 cut(s) 76
BseDI CCNNGG 2 cut(s) 242, 255
BseGI GGATG 3 cut(s) 313, 535, 625
BseLI CCNNNNNNNGG 2 cut(s) 64, 503
BseMI GCAATG 1 cut(s) 293
BseMII CTCAG 5 cut(s) 99, 186, 309, 378, 413
BseNI ACTGG 1 cut(s) 58
BseRI GAGGAG 2 cut(s) 337, 365
BseSI GKGCMC 1 cut(s) 134
BseXI GCAGC 4 cut(s) 298, 370, 448, 562
Bsh1236I CGCG 2 cut(s) 8, 634
BshFI GGCC 1 cut(s) 132
BsiHKAI GWGCWC 1 cut(s) 512
BsiSI CCGG 1 cut(s) 134
BslI CCNNNNNNNGG 2 cut(s) 64, 503
BsmI GAATGC 1 cut(s) 109
BsnI GGCC 1 cut(s) 132
Bsp119I TTCGAA 1 cut(s) 143
Bsp120I GGGCCC 1 cut(s) 130
Bsp1286I GDGCHC 2 cut(s) 134, 512
Bsp1720I GCTNAGC 1 cut(s) 606
BspACI CCGC 3 cut(s) 314, 372, 634
BspANI GGCC 1 cut(s) 132
BspCNI CTCAG 5 cut(s) 100, 187, 310, 379, 414
BspFNI CGCG 2 cut(s) 8, 634
BspLI GGNNCC 1 cut(s) 132
BspT104I TTCGAA 1 cut(s) 143
BsrDI GCAATG 1 cut(s) 293
BsrI ACTGG 1 cut(s) 58
BssECI CCNNGG 2 cut(s) 242, 255
BssNI GRCGYC 1 cut(s) 45
BssT1I CCWWGG 1 cut(s) 255
Bst2UI CCWGG 1 cut(s) 76
Bst4CI ACNGT 2 cut(s) 230, 286
Bst6I CTCTTC 3 cut(s) 20, 242, 283
BstACI GRCGYC 1 cut(s) 45
BstBI TTCGAA 1 cut(s) 143
BstC8I GCNNGC 1 cut(s) 441
BstDEI CTNAG 7 cut(s) 108, 195, 318, 387, 422, 556, 606
BstF5I GGATG 3 cut(s) 313, 535, 625
BstFNI CGCG 2 cut(s) 8, 634
BstHHI GCGC 2 cut(s) 8, 151
BstNI CCWGG 1 cut(s) 76
BstSCI CCNGG 2 cut(s) 74, 132
BstSFI CTRYAG 1 cut(s) 282
BstSLI GKGCMC 1 cut(s) 134
BstUI CGCG 2 cut(s) 8, 634
BstV1I GCAGC 4 cut(s) 298, 370, 448, 562
BsuRI GGCC 1 cut(s) 132
BtgZI GCGATG 1 cut(s) 402
BtsCI GGATG 3 cut(s) 313, 535, 625
Cac8I GCNNGC 1 cut(s) 441
CfoI GCGC 2 cut(s) 8, 151
Cfr13I GGNCC 3 cut(s) 130, 131, 405
Csp6I GTAC 2 cut(s) 94, 412
CviAII CATG 3 cut(s) 14, 152, 596
CviQI GTAC 2 cut(s) 94, 412
DdeI CTNAG 7 cut(s) 108, 195, 318, 387, 422, 556, 606
DriI GACNNNNNGTC 1 cut(s) 403
Eam1104I CTCTTC 3 cut(s) 20, 242, 283
Eam1105I GACNNNNNGTC 1 cut(s) 403
EarI CTCTTC 3 cut(s) 20, 242, 283
EciI GGCGGA 1 cut(s) 387
Ecl136II GAGCTC 1 cut(s) 510
Eco130I CCWWGG 1 cut(s) 255
Eco24I GRGCYC 2 cut(s) 134, 512
Eco47I GGWCC 1 cut(s) 405
Eco53kI GAGCTC 1 cut(s) 510
EcoICRI GAGCTC 1 cut(s) 510
EcoO109I RGGNCCY 1 cut(s) 130
EcoRII CCWGG 1 cut(s) 74
EcoT14I CCWWGG 1 cut(s) 255
EcoT22I ATGCAT 1 cut(s) 597
EcoT38I GRGCYC 2 cut(s) 134, 512
ErhI CCWWGG 1 cut(s) 255
FaeI CATG 3 cut(s) 17, 155, 599
FaiI YATR 5 cut(s) 15, 99, 153, 482, 597
FalI AAGNNNNNCTT 2 cut(s) 17, 49
FatI CATG 3 cut(s) 13, 151, 595
Fnu4HI GCNGC 5 cut(s) 312, 315, 384, 437, 551
FokI GGATG 3 cut(s) 320, 542, 632
FriOI GRGCYC 2 cut(s) 134, 512
Fsp4HI GCNGC 5 cut(s) 312, 315, 384, 437, 551
GlaI GCGC 2 cut(s) 7, 150
GluI GCNGC 5 cut(s) 312, 315, 384, 437, 551
HaeIII GGCC 1 cut(s) 132
HapII CCGG 1 cut(s) 134
HhaI GCGC 2 cut(s) 8, 151
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 3 cut(s) 17, 155, 599
Hin6I GCGC 2 cut(s) 6, 149
HinP1I GCGC 2 cut(s) 6, 149
HindIII AAGCTT 3 cut(s) 31, 581, 608
HinfI GANTC 3 cut(s) 389, 428, 484
HpaII CCGG 1 cut(s) 134
Hpy188I TCNGA 3 cut(s) 307, 394, 489
Hpy188III TCNNGA 2 cut(s) 22, 577
HpyAV CCTTC 4 cut(s) 31, 139, 507, 631
HpyCH4III ACNGT 2 cut(s) 230, 286
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 5 cut(s) 221, 436, 443, 550, 595
HpyF3I CTNAG 7 cut(s) 108, 195, 318, 387, 422, 556, 606
HpySE526I ACGT 1 cut(s) 45
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 3 cut(s) 17, 155, 599
HspAI GCGC 2 cut(s) 6, 149
LmnI GCTCC 2 cut(s) 191, 507
LpnPI CCDG 6 cut(s) 61, 71, 88, 147, 312, 587
Lsp1109I GCAGC 4 cut(s) 298, 370, 448, 562
LweI GCATC 2 cut(s) 298, 582
MaeII ACGT 1 cut(s) 45
MboII GAAGA 9 cut(s) 37, 97, 100, 190, 193, 247, 259, 270, 346
MfeI CAATTG 1 cut(s) 501
MhlI GDGCHC 2 cut(s) 134, 512
MluCI AATT 2 cut(s) 445, 501
MlyI GAGTC 1 cut(s) 398
MmeI TCCRAC 1 cut(s) 507
MnlI CCTC 9 cut(s) 72, 121, 190, 237, 315, 343, 450, 457, 534
Mph1103I ATGCAT 1 cut(s) 597
MseI TTAA 1 cut(s) 378
MslI CAYNNNNRTG 2 cut(s) 102, 457
MspI CCGG 1 cut(s) 134
MspR9I CCNGG 2 cut(s) 76, 134
MunI CAATTG 1 cut(s) 501
Mva1269I GAATGC 1 cut(s) 109
MvaI CCWGG 1 cut(s) 76
MvnI CGCG 2 cut(s) 8, 634
NciI CCSGG 1 cut(s) 134
NlaIII CATG 3 cut(s) 17, 155, 599
NlaIV GGNNCC 1 cut(s) 132
NmeAIII GCCGAG 1 cut(s) 267
NsiI ATGCAT 1 cut(s) 597
NspV TTCGAA 1 cut(s) 143
PctI GAATGC 1 cut(s) 109
PfeI GAWTC 2 cut(s) 428, 484
PflMI CCANNNNNTGG 1 cut(s) 64
PkrI GCNGC 5 cut(s) 313, 316, 385, 438, 552
PleI GAGTC 1 cut(s) 397
PpsI GAGTC 1 cut(s) 397
Psp124BI GAGCTC 1 cut(s) 512
Psp6I CCWGG 1 cut(s) 74
PspGI CCWGG 1 cut(s) 74
PspN4I GGNNCC 1 cut(s) 132
PspOMI GGGCCC 1 cut(s) 130
PspPI GGNCC 3 cut(s) 130, 131, 405
RsaI GTAC 2 cut(s) 95, 413
RsaNI GTAC 2 cut(s) 94, 412
RseI CAYNNNNRTG 2 cut(s) 102, 457
SacI GAGCTC 1 cut(s) 512
SaqAI TTAA 1 cut(s) 378
SatI GCNGC 5 cut(s) 312, 315, 384, 437, 551
Sau96I GGNCC 3 cut(s) 130, 131, 405
SchI GAGTC 1 cut(s) 398
ScrFI CCNGG 2 cut(s) 76, 134
SduI GDGCHC 2 cut(s) 134, 512
SfaNI GCATC 2 cut(s) 298, 582
SfcI CTRYAG 1 cut(s) 282
SfuI TTCGAA 1 cut(s) 143
SinI GGWCC 1 cut(s) 405
SmiMI CAYNNNNRTG 2 cut(s) 102, 457
Sse9I AATT 2 cut(s) 445, 501
SsiI CCGC 3 cut(s) 314, 372, 634
SstI GAGCTC 1 cut(s) 512
StyD4I CCNGG 2 cut(s) 74, 132
StyI CCWWGG 1 cut(s) 255
TaaI ACNGT 2 cut(s) 230, 286
TaiI ACGT 1 cut(s) 48
TaqI TCGA 3 cut(s) 143, 176, 455
TasI AATT 2 cut(s) 445, 501
TauI GCSGC 1 cut(s) 317
TfiI GAWTC 2 cut(s) 428, 484
Tru1I TTAA 1 cut(s) 378
Tru9I TTAA 1 cut(s) 378
TseI GCWGC 4 cut(s) 311, 383, 436, 550
TspDTI ATGAA 2 cut(s) 371, 612
Van91I CCANNNNNTGG 1 cut(s) 64
VpaK11BI GGWCC 1 cut(s) 405
ZraI GACGTC 1 cut(s) 46
Zsp2I ATGCAT 1 cut(s) 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.