Rmu_ssc0000182.1_g000011

SNF2 family N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000182.1
OG0004421
Physical Location & Seq
Forward (+)
41183 .. 49451
8269 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4374 bp
atgcgattgcaatcgacgcgatctgagtcgtcgccgtttctgaattcttcgtcggggtttgatcggaccagggttttacgacagcgcggcggcggaggaggaggagaggaggaggttagggagagttctaattgtggattcgccgccgaggatttcgacggcgaggacatgtcgatcgatctcgatacattctatcgtcttctcgaggaagaacccccgagttgtccggaggattcatcattgggaagcggatcacaggctgaagcagtttcaggtgctgattaccgtgaggatacactgctccaaagtggatcgcaagttttaaaagcagaatcatcagctgatttagggggtttcggatcatggcatcctccatacagctctgaagcttcggattctggggcagggcgtccaggtggttcctttgattatgcagggaaccctgcaatgttctctgactacaaagatagcaaaccaacagcatacactggctctcctggtccatggcatcctccatatatctcagaagcatcagattctgtggcagggggtccaggtggttcctttgagtgtggagggaaccctgcaatgtctattgacaggaaaccaccagcacagaccagctctcatgggcaaatgttccctacaagtcttaaagactggttttcgttggttccgtgcacggagacttacttcactgagagggttgacgtttcacagcccactagtagcaccacttctagttttgaagaaggacatactaatcatgttttagaccacggagatcttaatgtcctgcaggggaaagcagatgtggcagggaaagttgattctgaatattcttcacaaaaccttggtattgataacatggacatgaattccagaccatatggtgctttgggggaaaacaccccagaaaccttgggaccatcggaaaataattcttgtacatctatggaaataccctttctagatatagatatatgttcaccacatgttacttctaccgaatctactctctgtcaaaattctgatcttttcagtgaccattatacagctgcggatggtaggagcttagacaatagatatttggcagagtcctcaatccagcatagtccttatagctactactctacattatttccaagtaacaaagaaatggtgacgaatgtgaaggatgaatcactggagtttcacgctgatagttcatgctcaagcagtaagatgcatatcaattgtcaggagggaataaccggtacatatggctttgattcgccaatgattgatgcttcagatatcaaggagtggaattttgattatggaagatataattgtatttcggctgttagtgggaattcttcattggatgctgactcttgtcctgttgacaacaaggcttcagtcaagcctttgggttctactgagacatacatgtcaagcaaacgggaattcactggtgtgaaggatgaaaatattgatgagttggttgctcctagtactgatacgtttcattctttttcattcatggatgacagtagaaagccatcttacaatgctgatggtagctcttttgacaaggaaccaaagctctcaggttatgatgtatctactcagccttttggaaattcagggcatgctaaagaagagatgattgttgatactaagagaacttattattctgaaggtaatataaatggaagttctttaccccatgttggtggtggatatgtgaatctaaatggtttggaacatcagttgccagctgctcagccattctcttcaaacatgaatcagggttatagtacggacagacttgaggaagatgactatgatgtatgcattattgaagataagagtgatcctgcacccacacgtcgccttccagtggttagcaatactcgttacctggagcccttgaatcgctctctagcagttggaagtaacatcgttaattcacaacaatctagtgatcacgacacaggagtgggaggcataccattcaggacaagggaggagcaattgatcttgagagtagcattgcaggatctttcacagcccaagtcagaagctcttccaccagatggagttttaacagtacctctcttacgacatcagagaatcgcattgtcatggatggttcagaaggagacagctagcttgccttgttgcggaggaatacttgcagatgatcagggattgggaaaaacgatatcgacaattgcactcatacttaaggaaaggcctcctgcttctggagcctgtctagatgaaaaaaagtgcaagctagaaactctggatttggaccaggatgatgacatgcttcctgaggttagcagaaggaagcaagatgctgatgctcattcaagtgtctcaaatgaaacttctgagaagagcatgacatctttgatacaaacaaagggaaggccagcttgtggaacactcgttgtttgtcccaccagtgtcttgcggcaatgggcagaggagttgcgtaataaaataacagaaaaagctaaactctcagtgctagtctaccatggaggcaatcgaacgagggatccgtgtgagctagctaagtatgatgtggtcctcacaacatattcaattgtcagcatggaggtcccaaagcagcctcttgctgatggagaagatgaggagaaaggaaagcgagaggagtatgattctccacatatggggttttcatctaagaaaaggaaatatcctaataaatgctcaaagggtaagaagggattggagactgcagcgcttgagtctcttgctcgccctcttgctaaggttggatggtttagggttgtcttggatgaagcccagagtattaagaatcacagaactcaagtagctagggcctgttgggggcttcgagcgaaacgtaggtggtgcttatcggggacacctatccagaatgcgattgatgatctttatagctacttcagatttctgagatatgacccttatgcggtgtacaagtcgttttgtgcaaaaataaaatttccaattagtaagaatccagcaaaagggtacagaatgctgcaagctgttctgaagacaattatgttacgccgcacaaaaggcactcttcttgatggagaacctattattaatttaccgccaaaatttatagaactgaaaagggtggaattctccgcggaagaacgagatttttactcaaggctagagagtgattcacgcgctcaatttgaagaatatgcagctgctggaactgtgaaacaaaattatgttaatattttattgatgctcttgcgacttcgacaagcttgtgatcaccctcttcttgtcagacgttatgaatcacagtctttatggaaatcttcggttgagaaggcaaagaagcttactcatgacaaacaagtatcccttctgaattgtttggaagcttctttggcaatatgcggtatctgcaatgatgcacctgaagatgccgttgtttctgaatgtggtcatgtcttctgtagccaatgcatttttgactatctcactggtgatgacaaccagtgccccaacacaagttgcaaagttcgactgaatgtgtcttctgtcttttcaaaagccactttaaacagttcgctttctgaccaacctagtcaagggggcatgggttctgaagtttttgatgctgtagagtctttctatgaggatagctcgtacaattcatccaagattaaggctgcacttgaggttctctgcttgatgtgtaaaccaaagacttgcactacagaaaatagttgcttaccagaaagcgttgataaaaatgccagctgttctacaacgtcttttgacattgatggggctgagtcccttgaggacagttctgatggacagaacttgggtgtggacaaaagtcctaagaaaattgagaaggtagttcgagaaaaagcaatagtcttttcacagtggactaggatgctggacttgcttgaagcgtgtcttaaaacttccggccttgagtacagaagacttgatggaacaatgtcagttgttgccagagataaagctgtaaaggattttaacactcttcctgaggtgtcagttatgattatgtctttgaaagctgctagtcttggtctgaacatggttgcagcctgccatgtacttcttttggacctgtggtggaatcctacgactgaagatcaagcaattgatagagcacatcgtattgggcagactcgtcctgttacggttttacggttgactgtgagagatacggtcgaagatcgtattttagctcttcagaaaaagaagagagagatggtggcatctgcatttggagaggatgagactggtggtcgtcagactcgcttaacagttgatgatctcaagtacctgtttatgaagtga

Protein Analysis

1457

Amino Acids

160.38

Weight (kDa)

5.1

Isoelectric Point (pI)

55.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNF2-rel_dom PF00176 704 - 1102 7.1e-65 SNF2-related domain
zf-C3HC4_3 PF13920 1142 - 1180 5.5e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 1143 - 1179 9.6e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 1143 - 1179 1.5e-08 Zinc finger, C3HC4 type (RING finger)
Helicase_C PF00271 1299 - 1399 9.5e-20 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1432
AccB7I CCANNNNNTGG 2 cut(s) 2081, 2432
AccI GTMKAC 1 cut(s) 2529
AccII CGCG 4 cut(s) 19, 87, 3164, 3207
AccIII TCCGGA 1 cut(s) 226
AclWI GGATC 7 cut(s) 259, 319, 367, 1853, 2052, 2549, 2562
AcyI GRCGYC 1 cut(s) 409
AfeI AGCGCT 1 cut(s) 2763
AflII CTTAAG 1 cut(s) 2231
AflIII ACRYGT 4 cut(s) 168, 994, 1431, 1871
AgeI ACCGGT 1 cut(s) 1253
AhdI GACNNNNNGTC 1 cut(s) 4320
AhlI ACTAGT 1 cut(s) 725
AjiI CACGTC 1 cut(s) 1874
AjnI CCWGG 6 cut(s) 68, 412, 496, 553, 1905, 2304
AjuI GAANNNNNNNTTGG 2 cut(s) 1346, 1378
AleI CACNNNNGTG 2 cut(s) 1457, 1982
AloI GAACNNNNNNTCC 2 cut(s) 2539, 2571
Alw21I GWGCWC 2 cut(s) 683, 4186
Alw26I GTCTC 7 cut(s) 680, 1418, 2141, 2374, 2747, 2775, 4307
Alw44I GTGCAC 1 cut(s) 679
AlwI GGATC 7 cut(s) 259, 319, 367, 1853, 2052, 2549, 2562
AlwNI CAGNNNCTG 3 cut(s) 278, 539, 3233
Ama87I CYCGRG 2 cut(s) 203, 217
Aor13HI TCCGGA 1 cut(s) 226
Aor51HI AGCGCT 1 cut(s) 2763
AoxI GGCC 4 cut(s) 2240, 2423, 2862, 3976
ApaLI GTGCAC 1 cut(s) 679
AseI ATTAAT 1 cut(s) 3117
AsiGI ACCGGT 1 cut(s) 1253
Asp700I GAANNNNTTC 1 cut(s) 2070
AspLEI GCGC 3 cut(s) 87, 2764, 3209
AspS9I GGNCC 9 cut(s) 66, 500, 551, 926, 2302, 2584, 2617, 2862, 4138
AsuHPI GGTGA 4 cut(s) 981, 1174, 3293, 3530
AsuNHI GCTAGC 2 cut(s) 2153, 2566
AvaI CYCGRG 2 cut(s) 203, 217
AvaII GGWCC 8 cut(s) 66, 500, 551, 926, 2302, 2584, 2617, 4138
AxyI CCTNAGG 2 cut(s) 2325, 4056
BaeGI GKGCMC 2 cut(s) 683, 3536
BamHI GGATCC 1 cut(s) 2554
BanII GRGCYC 1 cut(s) 1914
BarI GAAGNNNNNNTAC 2 cut(s) 721, 753
BbsI GAAGAC 5 cut(s) 191, 3068, 3475, 3561, 3997
Bbv12I GWGCWC 2 cut(s) 683, 4186
BceAI ACGGC 3 cut(s) 19, 175, 3443
BciT130I CCWGG 6 cut(s) 70, 414, 498, 555, 1907, 2306
BciVI GTATCC 2 cut(s) 286, 3400
BclI TGATCA 3 cut(s) 1969, 2188, 3298
BcoDI GTCTC 7 cut(s) 680, 1418, 2141, 2374, 2747, 2775, 4307
BcuI ACTAGT 1 cut(s) 725
BfmI CTRYAG 5 cut(s) 797, 2757, 3487, 3654, 3750
BfoI RGCGCY 1 cut(s) 2765
BfrI CTTAAG 1 cut(s) 2231
BfuI GTATCC 2 cut(s) 286, 3400
BglII AGATCT 1 cut(s) 784
BlpI GCTNAGC 1 cut(s) 1767
BmcAI AGTACT 1 cut(s) 1498
Bme1390I CCNGG 6 cut(s) 70, 414, 498, 555, 1907, 2306
Bme18I GGWCC 8 cut(s) 66, 500, 551, 926, 2302, 2584, 2617, 4138
BmeRI GACNNNNNGTC 1 cut(s) 4320
BmeT110I CYCGRG 2 cut(s) 203, 217
BmgT120I GGNCC 9 cut(s) 66, 500, 551, 926, 2302, 2584, 2617, 2862, 4138
BmrFI CCNGG 6 cut(s) 70, 414, 498, 555, 1907, 2306
BmtI GCTAGC 2 cut(s) 2157, 2570
BoxI GACNNNNGTC 2 cut(s) 1377, 3876
BpiI GAAGAC 5 cut(s) 191, 3068, 3475, 3561, 3997
BplI GAGNNNNNCTC 2 cut(s) 3662, 3694
BpmI CTGGAG 3 cut(s) 1208, 1928, 2274
Bpu10I CCTNAGC 1 cut(s) 2790
Bpu1102I GCTNAGC 1 cut(s) 1767
Bsa29I ATCGAT 1 cut(s) 177
BsaBI GATNNNNATC 2 cut(s) 10, 1229
BsaHI GRCGYC 1 cut(s) 409
BsaJI CCNNGG 8 cut(s) 69, 147, 503, 778, 853, 921, 2533, 3162
BsaWI WCCGGW 2 cut(s) 226, 1253
BsaXI ACNNNNNCTCC 2 cut(s) 478, 508
Bse118I RCCGGY 1 cut(s) 1253
Bse1I ACTGG 9 cut(s) 493, 665, 1191, 1459, 1883, 2457, 3520, 3529, 4321
Bse21I CCTNAGG 2 cut(s) 2325, 4056
Bse3DI GCAATG 5 cut(s) 453, 594, 2036, 2477, 3445
Bse8I GATNNNNATC 2 cut(s) 10, 1229
BseAI TCCGGA 1 cut(s) 226
BseBI CCWGG 6 cut(s) 70, 414, 498, 555, 1907, 2306
BseCI ATCGAT 1 cut(s) 177
BseDI CCNNGG 8 cut(s) 69, 147, 503, 778, 853, 921, 2533, 3162
BseJI GATNNNNATC 2 cut(s) 10, 1229
BseMI GCAATG 5 cut(s) 453, 594, 2036, 2477, 3445
BseNI ACTGG 9 cut(s) 493, 665, 1191, 1459, 1883, 2457, 3520, 3529, 4321
BseRI GAGGAG 9 cut(s) 111, 114, 117, 122, 125, 2027, 2495, 2666, 2684
BseSI GKGCMC 2 cut(s) 683, 3536
BsgI GTGCAG 2 cut(s) 1848, 3690
Bsh1236I CGCG 4 cut(s) 19, 87, 3164, 3207
Bsh1285I CGRYCG 2 cut(s) 177, 4245
BshFI GGCC 4 cut(s) 2242, 2425, 2864, 3978
BshTI ACCGGT 1 cut(s) 1253
BshVI ATCGAT 1 cut(s) 177
BsiEI CGRYCG 2 cut(s) 177, 4245
BsiHKAI GWGCWC 2 cut(s) 683, 4186
BsiHKCI CYCGRG 2 cut(s) 203, 217
BsiSI CCGG 3 cut(s) 227, 1254, 3975
BslFI GGGAC 5 cut(s) 939, 2436, 2603, 2920, 3817
BsmAI GTCTC 7 cut(s) 680, 1418, 2141, 2374, 2747, 2775, 4307
BsmFI GGGAC 5 cut(s) 939, 2436, 2603, 2920, 3817
BsmI GAATGC 2 cut(s) 2926, 3048
BsnI GGCC 4 cut(s) 2242, 2425, 2864, 3978
BsoBI CYCGRG 2 cut(s) 203, 217
Bsp1286I GDGCHC 4 cut(s) 683, 1914, 3536, 4186
Bsp13I TCCGGA 1 cut(s) 226
Bsp1407I TGTACA 2 cut(s) 947, 2979
Bsp1720I GCTNAGC 1 cut(s) 1767
Bsp19I CCATGG 2 cut(s) 503, 2533
BspANI GGCC 4 cut(s) 2242, 2425, 2864, 3978
BspDI ATCGAT 1 cut(s) 177
BspEI TCCGGA 1 cut(s) 226
BspFNI CGCG 4 cut(s) 19, 87, 3164, 3207
BspHI TCATGA 1 cut(s) 3376
BspMAI CTGCAG 2 cut(s) 801, 2761
BspOI GCTAGC 2 cut(s) 2157, 2570
BspPI GGATC 7 cut(s) 259, 319, 367, 1853, 2052, 2549, 2562
BspQI GCTCTTC 3 cut(s) 2076, 2384, 4269
BspTI CTTAAG 1 cut(s) 2231
BsrDI GCAATG 5 cut(s) 453, 594, 2036, 2477, 3445
BsrFI RCCGGY 1 cut(s) 1253
BsrGI TGTACA 2 cut(s) 947, 2979
BsrI ACTGG 9 cut(s) 493, 665, 1191, 1459, 1883, 2457, 3520, 3529, 4321
BssAI RCCGGY 1 cut(s) 1253
BssECI CCNNGG 8 cut(s) 69, 147, 503, 778, 853, 921, 2533, 3162
BssNI GRCGYC 1 cut(s) 409
BssT1I CCWWGG 4 cut(s) 503, 853, 921, 2533
Bst2UI CCWGG 6 cut(s) 70, 414, 498, 555, 1907, 2306
Bst6I CTCTTC 9 cut(s) 1638, 1783, 2076, 2384, 3099, 3312, 4056, 4269, 4271
BstACI GRCGYC 1 cut(s) 409
BstAFI CTTAAG 1 cut(s) 2231
BstAUI TGTACA 2 cut(s) 947, 2979
BstDSI CCRYGG 4 cut(s) 503, 778, 2533, 3162
BstENI CCTNNNNNAGG 1 cut(s) 3621
BstFNI CGCG 4 cut(s) 19, 87, 3164, 3207
BstH2I RGCGCY 1 cut(s) 2765
BstHHI GCGC 3 cut(s) 87, 2764, 3209
BstMAI GTCTC 7 cut(s) 680, 1418, 2141, 2374, 2747, 2775, 4307
BstMCI CGRYCG 2 cut(s) 177, 4245
BstMWI GCNNNNNNNGC 7 cut(s) 16, 815, 2255, 3087, 3419, 3435, 3949
BstNI CCWGG 6 cut(s) 70, 414, 498, 555, 1907, 2306
BstNSI RCATGY 5 cut(s) 172, 998, 1435, 1637, 2320
BstPAI GACNNNNGTC 2 cut(s) 1377, 3876
BstSCI CCNGG 6 cut(s) 68, 412, 496, 553, 1905, 2304
BstSFI CTRYAG 5 cut(s) 797, 2757, 3487, 3654, 3750
BstSLI GKGCMC 2 cut(s) 683, 3536
BstUI CGCG 4 cut(s) 19, 87, 3164, 3207
BstV2I GAAGAC 5 cut(s) 191, 3068, 3475, 3561, 3997
BstX2I RGATCY 3 cut(s) 784, 2044, 2554
BstXI CCANNNNNNTGG 1 cut(s) 1718
BstYI RGATCY 3 cut(s) 784, 2044, 2554
Bsu15I ATCGAT 1 cut(s) 177
Bsu36I CCTNAGG 2 cut(s) 2325, 4056
BsuI GTATCC 2 cut(s) 286, 3400
BsuRI GGCC 4 cut(s) 2242, 2425, 2864, 3978
BsuTUI ATCGAT 1 cut(s) 177
BtgI CCRYGG 4 cut(s) 503, 778, 2533, 3162
BtrI CACGTC 1 cut(s) 1874
BtsI GCAGTG 1 cut(s) 296
CaiI CAGNNNCTG 3 cut(s) 278, 539, 3233
CciI TCATGA 1 cut(s) 3376
CfoI GCGC 3 cut(s) 87, 2764, 3209
Cfr10I RCCGGY 1 cut(s) 1253
Cfr13I GGNCC 9 cut(s) 66, 500, 551, 926, 2302, 2584, 2617, 2862, 4138
Cfr42I CCGCGG 1 cut(s) 3165
ClaI ATCGAT 1 cut(s) 177
CseI GACGC 2 cut(s) 25, 398
CspAI ACCGGT 1 cut(s) 1253
DraI TTTAAA 2 cut(s) 324, 3594
DrdI GACNNNNNNGTC 1 cut(s) 1432
DriI GACNNNNNGTC 1 cut(s) 4320
DseDI GACNNNNNNGTC 1 cut(s) 1432
Eam1104I CTCTTC 9 cut(s) 1638, 1783, 2076, 2384, 3099, 3312, 4056, 4269, 4271
Eam1105I GACNNNNNGTC 1 cut(s) 4320
EarI CTCTTC 9 cut(s) 1638, 1783, 2076, 2384, 3099, 3312, 4056, 4269, 4271
EciI GGCGGA 1 cut(s) 108
Eco130I CCWWGG 4 cut(s) 503, 853, 921, 2533
Eco147I AGGCCT 1 cut(s) 2242
Eco24I GRGCYC 1 cut(s) 1914
Eco32I GATATC 2 cut(s) 1297, 2211
Eco47I GGWCC 8 cut(s) 66, 500, 551, 926, 2302, 2584, 2617, 4138
Eco47III AGCGCT 1 cut(s) 2763
Eco81I CCTNAGG 2 cut(s) 2325, 4056
Eco88I CYCGRG 2 cut(s) 203, 217
EcoNI CCTNNNNNAGG 1 cut(s) 3621
EcoO109I RGGNCCY 2 cut(s) 2617, 2862
EcoRI GAATTC 5 cut(s) 43, 877, 1354, 1448, 3155
EcoRII CCWGG 6 cut(s) 68, 412, 496, 553, 1905, 2304
EcoRV GATATC 2 cut(s) 1297, 2211
EcoT14I CCWWGG 4 cut(s) 503, 853, 921, 2533
EcoT22I ATGCAT 3 cut(s) 1230, 1841, 3500
EcoT38I GRGCYC 1 cut(s) 1914
ErhI CCWWGG 4 cut(s) 503, 853, 921, 2533
FalI AAGNNNNNCTT 8 cut(s) 2413, 2445, 3078, 3110, 3378, 3410, 3948, 3980
FaqI GGGAC 5 cut(s) 939, 2436, 2603, 2920, 3817
FauNDI CATATG 3 cut(s) 889, 1261, 2688
FbaI TGATCA 3 cut(s) 1969, 2188, 3298
FblI GTMKAC 1 cut(s) 2529
FriOI GRGCYC 1 cut(s) 1914
GlaI GCGC 3 cut(s) 86, 2763, 3208
GsuI CTGGAG 3 cut(s) 1208, 1928, 2274
HaeII RGCGCY 1 cut(s) 2765
HaeIII GGCC 4 cut(s) 2242, 2425, 2864, 3978
HapII CCGG 3 cut(s) 227, 1254, 3975
HgaI GACGC 2 cut(s) 25, 398
HhaI GCGC 3 cut(s) 87, 2764, 3209
Hin1I GRCGYC 1 cut(s) 409
Hin6I GCGC 3 cut(s) 85, 2762, 3207
HinP1I GCGC 3 cut(s) 85, 2762, 3207
HincII GTYRAC 3 cut(s) 709, 1387, 4227
HindII GTYRAC 3 cut(s) 709, 1387, 4227
HindIII AAGCTT 4 cut(s) 387, 3291, 3368, 3411
HpaII CCGG 3 cut(s) 227, 1254, 3975
HphI GGTGA 4 cut(s) 981, 1174, 3293, 3530
Hpy99I CGWCG 5 cut(s) 19, 34, 55, 161, 1878
HpyCH4IV ACGT 6 cut(s) 711, 1505, 1873, 2887, 3319, 3806
HpyF10VI GCNNNNNNNGC 7 cut(s) 16, 815, 2255, 3087, 3419, 3435, 3949
HpySE526I ACGT 6 cut(s) 711, 1505, 1873, 2887, 3319, 3806
Hsp92I GRCGYC 1 cut(s) 409
HspAI GCGC 3 cut(s) 85, 2762, 3207
Kpn2I TCCGGA 1 cut(s) 226
Ksp22I TGATCA 3 cut(s) 1969, 2188, 3298
KspI CCGCGG 1 cut(s) 3165
LguI GCTCTTC 3 cut(s) 2076, 2384, 4269
LmnI GCTCC 6 cut(s) 306, 1071, 1495, 1909, 2014, 2255
MaeII ACGT 6 cut(s) 711, 1505, 1873, 2887, 3319, 3806
MaeIII GTNAC 8 cut(s) 997, 1043, 1148, 1162, 1901, 1940, 3072, 4210
MfeI CAATTG 5 cut(s) 1234, 2018, 2217, 2601, 4173
MflI RGATCY 3 cut(s) 784, 2044, 2554
MhlI GDGCHC 4 cut(s) 683, 1914, 3536, 4186
MlyI GAGTC 8 cut(s) 35, 1106, 1367, 2777, 3668, 3839, 4195, 4324
MmeI TCCRAC 2 cut(s) 1915, 2776
Mph1103I ATGCAT 3 cut(s) 1230, 1841, 3500
MroI TCCGGA 1 cut(s) 226
MroXI GAANNNNTTC 1 cut(s) 2070
MslI CAYNNNNRTG 6 cut(s) 872, 1457, 1716, 1982, 2128, 2364
MspA1I CMGCKG 6 cut(s) 341, 1058, 1763, 3164, 3230, 3795
MspCI CTTAAG 1 cut(s) 2231
MspI CCGG 3 cut(s) 227, 1254, 3975
MspR9I CCNGG 6 cut(s) 70, 414, 498, 555, 1907, 2306
MunI CAATTG 5 cut(s) 1234, 2018, 2217, 2601, 4173
Mva1269I GAATGC 2 cut(s) 2926, 3048
MvaI CCWGG 6 cut(s) 70, 414, 498, 555, 1907, 2306
MvnI CGCG 4 cut(s) 19, 87, 3164, 3207
MwoI GCNNNNNNNGC 7 cut(s) 16, 815, 2255, 3087, 3419, 3435, 3949
NcoI CCATGG 2 cut(s) 503, 2533
NdeI CATATG 3 cut(s) 889, 1261, 2688
NheI GCTAGC 2 cut(s) 2153, 2566
NmeAIII GCCGAG 1 cut(s) 172
NmuCI GTSAC 2 cut(s) 1043, 1162
NsiI ATGCAT 3 cut(s) 1230, 1841, 3500
NspI RCATGY 5 cut(s) 172, 998, 1435, 1637, 2320
OliI CACNNNNGTG 2 cut(s) 1457, 1982
PaeI GCATGC 1 cut(s) 1637
PaeR7I CTCGAG 1 cut(s) 203
PagI TCATGA 1 cut(s) 3376
PceI AGGCCT 1 cut(s) 2242
PciI ACATGT 3 cut(s) 168, 994, 1431
PciSI GCTCTTC 3 cut(s) 2076, 2384, 4269
PcsI WCGNNNNNNNCGW 3 cut(s) 674, 2555, 2884
PctI GAATGC 2 cut(s) 2926, 3048
PdmI GAANNNNTTC 1 cut(s) 2070
PflMI CCANNNNNTGG 2 cut(s) 2081, 2432
PinAI ACCGGT 1 cut(s) 1253
Ple19I CGATCG 1 cut(s) 177
PleI GAGTC 8 cut(s) 34, 1105, 1367, 2776, 3667, 3838, 4195, 4324
PpsI GAGTC 8 cut(s) 34, 1105, 1367, 2776, 3667, 3838, 4195, 4324
PpuMI RGGWCCY 1 cut(s) 2617
PscI ACATGT 3 cut(s) 168, 994, 1431
PshAI GACNNNNGTC 2 cut(s) 1377, 3876
PshBI ATTAAT 1 cut(s) 3117
Psp5II RGGWCCY 1 cut(s) 2617
Psp6I CCWGG 6 cut(s) 68, 412, 496, 553, 1905, 2304
PspGI CCWGG 6 cut(s) 68, 412, 496, 553, 1905, 2304
PspPI GGNCC 9 cut(s) 66, 500, 551, 926, 2302, 2584, 2617, 2862, 4138
PspPPI RGGWCCY 1 cut(s) 2617
PstI CTGCAG 2 cut(s) 801, 2761
PstNI CAGNNNCTG 3 cut(s) 278, 539, 3233
PsuI RGATCY 3 cut(s) 784, 2044, 2554
PvuI CGATCG 1 cut(s) 177
PvuII CAGCTG 5 cut(s) 341, 1058, 1763, 3230, 3795
RseI CAYNNNNRTG 6 cut(s) 872, 1457, 1716, 1982, 2128, 2364
SacII CCGCGG 1 cut(s) 3165
SapI GCTCTTC 3 cut(s) 2076, 2384, 4269
Sau96I GGNCC 9 cut(s) 66, 500, 551, 926, 2302, 2584, 2617, 2862, 4138
SbfI CCTGCAGG 1 cut(s) 801
ScaI AGTACT 1 cut(s) 1498
SchI GAGTC 8 cut(s) 35, 1106, 1367, 2777, 3668, 3839, 4195, 4324
ScrFI CCNGG 6 cut(s) 70, 414, 498, 555, 1907, 2306
SdaI CCTGCAGG 1 cut(s) 801
SduI GDGCHC 4 cut(s) 683, 1914, 3536, 4186
SfcI CTRYAG 5 cut(s) 797, 2757, 3487, 3654, 3750
Sfr274I CTCGAG 1 cut(s) 203
Sfr303I CCGCGG 1 cut(s) 3165
SgrBI CCGCGG 1 cut(s) 3165
SinI GGWCC 8 cut(s) 66, 500, 551, 926, 2302, 2584, 2617, 4138
SlaI CTCGAG 1 cut(s) 203
SmiMI CAYNNNNRTG 6 cut(s) 872, 1457, 1716, 1982, 2128, 2364
SpeI ACTAGT 1 cut(s) 725
SphI GCATGC 1 cut(s) 1637
Sse8387I CCTGCAGG 1 cut(s) 801
SseBI AGGCCT 1 cut(s) 2242
SspI AATATT 3 cut(s) 839, 1474, 3262
StuI AGGCCT 1 cut(s) 2242
StyD4I CCNGG 6 cut(s) 68, 412, 496, 553, 1905, 2304
StyI CCWWGG 4 cut(s) 503, 853, 921, 2533
TaiI ACGT 6 cut(s) 714, 1508, 1876, 2890, 3322, 3809
TatI WGTACW 5 cut(s) 947, 1496, 2979, 3984, 4126
TauI GCSGC 5 cut(s) 90, 93, 146, 2470, 3081
TseFI GTSAC 2 cut(s) 1043, 1162
Tsp45I GTSAC 2 cut(s) 1043, 1162
TspGWI ACGGA 5 cut(s) 666, 698, 795, 1820, 2547
Van91I CCANNNNNTGG 2 cut(s) 2081, 2432
Vha464I CTTAAG 1 cut(s) 2231
VneI GTGCAC 1 cut(s) 679
VpaK11BI GGWCC 8 cut(s) 66, 500, 551, 926, 2302, 2584, 2617, 4138
VspI ATTAAT 1 cut(s) 3117
XagI CCTNNNNNAGG 1 cut(s) 3621
XbaI TCTAGA 2 cut(s) 970, 2263
XceI RCATGY 5 cut(s) 172, 998, 1435, 1637, 2320
XcmI CCANNNNNNNNNTGG 1 cut(s) 1718
XhoI CTCGAG 1 cut(s) 203
XmiI GTMKAC 1 cut(s) 2529
XmnI GAANNNNTTC 1 cut(s) 2070
ZrmI AGTACT 1 cut(s) 1498
Zsp2I ATGCAT 3 cut(s) 1230, 1841, 3500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.