Rmu_ssc0000204.1_g000042

Vacuolar iron transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000204.1
OG0002581
Physical Location & Seq
Reverse (-)
192337 .. 193896
1560 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 708 bp
atgggtagtgatcttgacaatggctatggtgacttggagaagcagatgctggttcttcacgaacacgaagagaagcacttcatgtccagtgagattgtccgtgacgtcattctcggcgtctccgacggtctcactgtcccttttgccctcgccgcggggttgtccggcgctgatgtcagctcctccattatcctcattgccggcattgctgaggttgccgccggtgccatctccatgggacttggcgggtatcttgcggcgaagagtgaagaagatcattacaagaaagaactaaagagggagcaagatgaaatcatcgccgtccctgatatagcacgtgaatatgggcctgtggtgaatgctctaagaaggaaccctcaagcttggcttgatttcatgatgaaatttgaactaggactcgacaagccagacccaatgagagcgctgcagagtgcaatgacaatagccttttcatacgtctgtggtggactggtgcctctccttccctacatgctcattccagttgctacaaaggcggtgcttgcatctgtggtggtcaccatagtggcattgctgatcttcggctttgcaaagggtcacttcaccggaactcaacctttcaagagcgctttccaaaccgccttcattggagccatcgcatccgccgcagcttattccattgcgaaactcttccaatcctaccactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VIT1

Protein Analysis

235

Amino Acids

25.2

Weight (kDa)

5.33

Isoelectric Point (pI)

38.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VIT1 PF01988 33 - 228 1.7e-60 VIT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 108
AccB1I GGYRCC 2 cut(s) 224, 493
AccII CGCG 1 cut(s) 155
AciI CCGC 9 cut(s) 153, 155, 219, 246, 257, 536, 639, 663, 666
AcsI RAATTY 1 cut(s) 404
AcvI CACGTG 1 cut(s) 338
AcyI GRCGYC 2 cut(s) 105, 117
AfeI AGCGCT 2 cut(s) 444, 628
AfiI CCNNNNNNNGG 1 cut(s) 154
AgsI TTSAA 2 cut(s) 410, 622
AleI CACNNNNGTG 1 cut(s) 563
AluBI AGCT 3 cut(s) 180, 383, 671
AluI AGCT 3 cut(s) 180, 383, 671
Alw26I GTCTC 2 cut(s) 124, 134
AlwNI CAGNNNCTG 1 cut(s) 49
Aor51HI AGCGCT 2 cut(s) 444, 628
AoxI GGCC 1 cut(s) 347
ApeKI GCWGC 2 cut(s) 445, 668
ApoI RAATTY 1 cut(s) 404
Asp700I GAANNNNTTC 2 cut(s) 77, 689
AspLEI GCGC 3 cut(s) 170, 445, 629
AspS9I GGNCC 1 cut(s) 347
AsuHPI GGTGA 4 cut(s) 41, 367, 550, 595
BanI GGYRCC 2 cut(s) 224, 493
BbrPI CACGTG 1 cut(s) 338
BbvCI CCTCAGC 1 cut(s) 210
BbvI GCAGC 2 cut(s) 432, 680
BccI CCATC 2 cut(s) 236, 662
BceAI ACGGC 1 cut(s) 305
BcoDI GTCTC 2 cut(s) 124, 134
BfaI CTAG 1 cut(s) 413
BfmI CTRYAG 1 cut(s) 446
BfoI RGCGCY 3 cut(s) 171, 446, 630
BisI GCNGC 6 cut(s) 153, 219, 258, 446, 666, 669
BlsI GCNGC 6 cut(s) 154, 220, 259, 447, 667, 670
BmgT120I GGNCC 1 cut(s) 347
BmiI GGNNCC 4 cut(s) 226, 374, 495, 652
BmsI GCATC 3 cut(s) 36, 554, 668
Bpu10I CCTNAGC 1 cut(s) 210
BpuEI CTTGAG 1 cut(s) 363
BsaAI YACGTR 1 cut(s) 338
BsaHI GRCGYC 2 cut(s) 105, 117
BsaI GGTCTC 1 cut(s) 134
BsaJI CCNNGG 2 cut(s) 153, 234
BsaWI WCCGGW 1 cut(s) 605
Bsc4I CCNNNNNNNGG 1 cut(s) 154
Bse118I RCCGGY 2 cut(s) 200, 221
Bse1I ACTGG 3 cut(s) 87, 495, 521
Bse3DI GCAATG 5 cut(s) 195, 204, 462, 569, 678
BseDI CCNNGG 2 cut(s) 153, 234
BseGI GGATG 1 cut(s) 659
BseLI CCNNNNNNNGG 1 cut(s) 154
BseMI GCAATG 5 cut(s) 195, 204, 462, 569, 678
BseMII CTCAG 1 cut(s) 201
BseNI ACTGG 3 cut(s) 87, 495, 521
BseRI GAGGAG 1 cut(s) 172
BseXI GCAGC 2 cut(s) 432, 680
Bsh1236I CGCG 1 cut(s) 155
BshFI GGCC 1 cut(s) 349
BshNI GGYRCC 2 cut(s) 224, 493
BsiSI CCGG 4 cut(s) 165, 201, 222, 606
BslFI GGGAC 3 cut(s) 122, 252, 308
BslI CCNNNNNNNGG 1 cut(s) 154
BsmAI GTCTC 2 cut(s) 124, 134
BsmBI CGTCTC 1 cut(s) 124
BsmFI GGGAC 3 cut(s) 122, 252, 308
BsmI GAATGC 1 cut(s) 364
BsnI GGCC 1 cut(s) 349
Bso31I GGTCTC 1 cut(s) 134
Bsp143I GATC 3 cut(s) 10, 274, 576
Bsp19I CCATGG 1 cut(s) 234
BspACI CCGC 9 cut(s) 153, 155, 219, 246, 257, 536, 639, 663, 666
BspANI GGCC 1 cut(s) 349
BspCNI CTCAG 1 cut(s) 202
BspFNI CGCG 1 cut(s) 155
BspHI TCATGA 1 cut(s) 396
BspLI GGNNCC 4 cut(s) 226, 374, 495, 652
BspMAI CTGCAG 1 cut(s) 450
BspT107I GGYRCC 2 cut(s) 224, 493
BspTNI GGTCTC 1 cut(s) 134
BsrDI GCAATG 5 cut(s) 195, 204, 462, 569, 678
BsrFI RCCGGY 2 cut(s) 200, 221
BsrI ACTGG 3 cut(s) 87, 495, 521
BssAI RCCGGY 2 cut(s) 200, 221
BssECI CCNNGG 2 cut(s) 153, 234
BssMI GATC 3 cut(s) 10, 274, 576
BssNI GRCGYC 2 cut(s) 105, 117
BssT1I CCWWGG 1 cut(s) 234
Bst4CI ACNGT 2 cut(s) 128, 136
Bst6I CTCTTC 3 cut(s) 63, 257, 695
BstACI GRCGYC 2 cut(s) 105, 117
BstBAI YACGTR 1 cut(s) 338
BstC8I GCNNGC 2 cut(s) 202, 543
BstDEI CTNAG 2 cut(s) 210, 365
BstDSI CCRYGG 2 cut(s) 153, 234
BstEII GGTNACC 1 cut(s) 556
BstF5I GGATG 1 cut(s) 659
BstFNI CGCG 1 cut(s) 155
BstH2I RGCGCY 3 cut(s) 171, 446, 630
BstHHI GCGC 3 cut(s) 170, 445, 629
BstKTI GATC 3 cut(s) 13, 277, 579
BstMAI GTCTC 2 cut(s) 124, 134
BstMBI GATC 3 cut(s) 10, 274, 576
BstMWI GCNNNNNNNGC 7 cut(s) 152, 206, 215, 224, 533, 542, 665
BstNSI RCATGY 1 cut(s) 514
BstPI GGTNACC 1 cut(s) 556
BstSFI CTRYAG 1 cut(s) 446
BstUI CGCG 1 cut(s) 155
BstV1I GCAGC 2 cut(s) 432, 680
BstXI CCANNNNNNTGG 1 cut(s) 235
BsuRI GGCC 1 cut(s) 349
BtgI CCRYGG 2 cut(s) 153, 234
BtgZI GCGATG 2 cut(s) 301, 640
BtsCI GGATG 1 cut(s) 659
BtsIMutI CAGTG 2 cut(s) 94, 132
Cac8I GCNNGC 2 cut(s) 202, 543
CaiI CAGNNNCTG 1 cut(s) 49
CciI TCATGA 1 cut(s) 396
CfoI GCGC 3 cut(s) 170, 445, 629
Cfr10I RCCGGY 2 cut(s) 200, 221
Cfr13I GGNCC 1 cut(s) 347
Cfr42I CCGCGG 1 cut(s) 156
CseI GACGC 1 cut(s) 106
CviAII CATG 4 cut(s) 82, 235, 397, 511
DdeI CTNAG 2 cut(s) 210, 365
DpnI GATC 3 cut(s) 12, 276, 578
DpnII GATC 3 cut(s) 10, 274, 576
Eam1104I CTCTTC 3 cut(s) 63, 257, 695
EarI CTCTTC 3 cut(s) 63, 257, 695
EciI GGCGGA 1 cut(s) 652
Eco130I CCWWGG 1 cut(s) 234
Eco31I GGTCTC 1 cut(s) 134
Eco47III AGCGCT 2 cut(s) 444, 628
Eco72I CACGTG 1 cut(s) 338
Eco91I GGTNACC 1 cut(s) 556
EcoO65I GGTNACC 1 cut(s) 556
EcoT14I CCWWGG 1 cut(s) 234
ErhI CCWWGG 1 cut(s) 234
Esp3I CGTCTC 1 cut(s) 124
FaeI CATG 4 cut(s) 85, 238, 400, 514
FaiI YATR 9 cut(s) 27, 83, 236, 332, 345, 398, 475, 512, 563
FalI AAGNNNNNCTT 4 cut(s) 372, 404, 584, 616
FaqI GGGAC 3 cut(s) 122, 252, 308
FatI CATG 4 cut(s) 81, 234, 396, 510
FauI CCCGC 2 cut(s) 148, 239
Fnu4HI GCNGC 6 cut(s) 153, 219, 258, 446, 666, 669
FokI GGATG 1 cut(s) 646
Fsp4HI GCNGC 6 cut(s) 153, 219, 258, 446, 666, 669
FspBI CTAG 1 cut(s) 413
GlaI GCGC 3 cut(s) 169, 444, 628
GluI GCNGC 6 cut(s) 153, 219, 258, 446, 666, 669
HaeII RGCGCY 3 cut(s) 171, 446, 630
HaeIII GGCC 1 cut(s) 349
HapII CCGG 4 cut(s) 165, 201, 222, 606
HgaI GACGC 1 cut(s) 106
HhaI GCGC 3 cut(s) 170, 445, 629
Hin1I GRCGYC 2 cut(s) 105, 117
Hin1II CATG 4 cut(s) 85, 238, 400, 514
Hin6I GCGC 3 cut(s) 168, 443, 627
HinP1I GCGC 3 cut(s) 168, 443, 627
HindIII AAGCTT 1 cut(s) 381
HinfI GANTC 1 cut(s) 417
HpaII CCGG 4 cut(s) 165, 201, 222, 606
HphI GGTGA 4 cut(s) 41, 367, 550, 595
Hpy166II GTNNAC 1 cut(s) 488
Hpy188I TCNGA 1 cut(s) 124
Hpy188III TCNNGA 4 cut(s) 14, 59, 397, 622
Hpy8I GTNNAC 1 cut(s) 488
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 3 cut(s) 363, 512, 652
HpyCH4III ACNGT 2 cut(s) 128, 136
HpyCH4IV ACGT 3 cut(s) 105, 337, 477
HpyCH4V TGCA 4 cut(s) 448, 455, 545, 590
HpyF10VI GCNNNNNNNGC 7 cut(s) 152, 206, 215, 224, 533, 542, 665
HpyF3I CTNAG 2 cut(s) 210, 365
HpySE526I ACGT 3 cut(s) 105, 337, 477
Hsp92I GRCGYC 2 cut(s) 105, 117
Hsp92II CATG 4 cut(s) 85, 238, 400, 514
HspAI GCGC 3 cut(s) 168, 443, 627
KroI GCCGGC 1 cut(s) 200
KroNI GCCGGC 1 cut(s) 202
KspI CCGCGG 1 cut(s) 156
Kzo9I GATC 3 cut(s) 10, 274, 576
LmnI GCTCC 3 cut(s) 185, 301, 650
Lsp1109I GCAGC 2 cut(s) 432, 680
LweI GCATC 3 cut(s) 36, 554, 668
MaeI CTAG 1 cut(s) 413
MaeII ACGT 3 cut(s) 105, 337, 477
MaeIII GTNAC 4 cut(s) 29, 101, 556, 596
MalI GATC 3 cut(s) 12, 276, 578
MboI GATC 3 cut(s) 10, 274, 576
MboII GAAGA 7 cut(s) 47, 80, 274, 281, 284, 571, 682
MluCI AATT 1 cut(s) 404
MlyI GAGTC 1 cut(s) 411
MmeI TCCRAC 1 cut(s) 147
MnlI CCTC 7 cut(s) 158, 193, 203, 205, 291, 387, 507
MroNI GCCGGC 1 cut(s) 200
MroXI GAANNNNTTC 2 cut(s) 77, 689
MslI CAYNNNNRTG 2 cut(s) 233, 563
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 4 cut(s) 165, 201, 222, 606
Mva1269I GAATGC 1 cut(s) 364
MvnI CGCG 1 cut(s) 155
MwoI GCNNNNNNNGC 7 cut(s) 152, 206, 215, 224, 533, 542, 665
NaeI GCCGGC 1 cut(s) 202
NcoI CCATGG 1 cut(s) 234
NdeII GATC 3 cut(s) 10, 274, 576
NgoMIV GCCGGC 1 cut(s) 200
NlaIII CATG 4 cut(s) 85, 238, 400, 514
NlaIV GGNNCC 4 cut(s) 226, 374, 495, 652
NmeAIII GCCGAG 1 cut(s) 93
NmuCI GTSAC 4 cut(s) 29, 101, 556, 596
NspI RCATGY 1 cut(s) 514
OliI CACNNNNGTG 1 cut(s) 563
PagI TCATGA 1 cut(s) 396
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PctI GAATGC 1 cut(s) 364
PdiI GCCGGC 1 cut(s) 202
PdmI GAANNNNTTC 2 cut(s) 77, 689
PkrI GCNGC 6 cut(s) 154, 220, 259, 447, 667, 670
PleI GAGTC 1 cut(s) 411
PmaCI CACGTG 1 cut(s) 338
PmlI CACGTG 1 cut(s) 338
PpsI GAGTC 1 cut(s) 411
Ppu21I YACGTR 1 cut(s) 338
PspCI CACGTG 1 cut(s) 338
PspEI GGTNACC 1 cut(s) 556
PspN4I GGNNCC 4 cut(s) 226, 374, 495, 652
PspPI GGNCC 1 cut(s) 347
PstI CTGCAG 1 cut(s) 450
PstNI CAGNNNCTG 1 cut(s) 49
RseI CAYNNNNRTG 2 cut(s) 233, 563
SacII CCGCGG 1 cut(s) 156
SatI GCNGC 6 cut(s) 153, 219, 258, 446, 666, 669
Sau3AI GATC 3 cut(s) 10, 274, 576
Sau96I GGNCC 1 cut(s) 347
SchI GAGTC 1 cut(s) 411
SetI ASST 8 cut(s) 108, 182, 216, 340, 385, 480, 619, 673
SfaNI GCATC 3 cut(s) 36, 554, 668
SfcI CTRYAG 1 cut(s) 446
Sfr303I CCGCGG 1 cut(s) 156
SgrAI CRCCGGYG 1 cut(s) 221
SgrBI CCGCGG 1 cut(s) 156
SmiMI CAYNNNNRTG 2 cut(s) 233, 563
SmlI CTYRAG 1 cut(s) 378
SmoI CTYRAG 1 cut(s) 378
Sse9I AATT 1 cut(s) 404
SsiI CCGC 9 cut(s) 153, 155, 219, 246, 257, 536, 639, 663, 666
SspMI CTAG 1 cut(s) 413
StyI CCWWGG 1 cut(s) 234
TaaI ACNGT 2 cut(s) 128, 136
TaiI ACGT 3 cut(s) 108, 340, 480
TaqI TCGA 1 cut(s) 420
TasI AATT 1 cut(s) 404
TauI GCSGC 4 cut(s) 155, 221, 260, 668
TscAI CASTG 2 cut(s) 94, 139
TseFI GTSAC 4 cut(s) 29, 101, 556, 596
TseI GCWGC 2 cut(s) 445, 668
Tsp45I GTSAC 4 cut(s) 29, 101, 556, 596
TspDTI ATGAA 6 cut(s) 70, 324, 385, 416, 462, 634
TspGWI ACGGA 1 cut(s) 89
TspRI CASTG 2 cut(s) 94, 139
XapI RAATTY 1 cut(s) 404
XceI RCATGY 1 cut(s) 514
XmnI GAANNNNTTC 2 cut(s) 77, 689
XspI CTAG 1 cut(s) 413
ZraI GACGTC 1 cut(s) 106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.