Rmu_ssc0000236.1_g000002

Protein NRDE2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000236.1
OG0010115
Physical Location & Seq
Reverse (-)
2000 .. 8831
6832 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3456 bp
atggacgacgacaggccgtcgccgtcgctgttcccagtcatagccgcctctcagcaagtctcaaacgtccctcaatggctttccaacaccagcttcaccaccaacctctccgtcataaacgacgccgttgcctcgcacttcaaacctgagccaccgccaccgccaccgccggaggagcaagtcgacgtacggccccagccgaagccgtacgagcttctggaatcttctagcggatccgaagcgagcgatgagagagacaggacgagcaagaaggagaaggggaagaggaggaagaagaggcggagcgcgcagagagacggcgggtcgttcgccggtttcgggtcgagaaagtccagtgttcgggcatgggccgaatccgagactagaccgtcggaaaattattacttggattccagtggcgaccttgataatttggcctttggatgtctctataggatggacattgctcgatacaaaccttatgctgctctttcggatagctccgggaattttaaagctttgtatcaggggaatcagacaatttcaacattggatagagatgctgatgttgatgcattggatggtaaactaaaatccgggggtcgctattggtctgcaaagtacatggctttggaacgccataagaacttaaagcgccttcgtttacttgcccctagagaccttgcggatacagtttcaggtgattttattcctttgacggatattcaaacatccgatgaaggtgaaggtgttgctgctgatgagtcactctcgggaactccagtagtggtcgaagaatcatgggaagatgaattgctacgcaagactcgggagtttaacaaattgacgagggagcatcctcatgatgagaaggtttggttggcttttgctgagttccaagacaaggttgctgatatgcagccccagaaaggtgctcgcttgcagactctggaaaaaaagattagcatattggagaaagccacagagcttaacccagataatgaagaactgttgctttgtcttctaaaggcttataaaagcagagacagttccgatgtattaattagtagatgggaaaagatactcatacagcattcaggaaattaccacttgtggagagagtttctgcatgttattcaaggggagttctccaggttcaaggtttcagacatgagaaaaatgtacgcacatgcaatccaggctatatctgctgcatgcagaatgcactctaggcaggtttgccaaggagaaaaatcttctccagatcccgctattgttcaactagaacttggtctggtggatatttttctcagttattgtaggtttgaatggcaggttggctaccaagagttggccaccgctctatttcaggctgaaattgaattcagtttgttttgtccttctttgctcctcactgagcagagtaagcagatattatttgagcacttctggaacagtgatggtgctagagttggagaagaaggcgctcttggttggtccacatggttggagaaagaggaagaaaataggcaaagggtcatcagagaggagatggcacatgataatgaaggtggttggacaggttggtctgaaccactatcaaaaaataaggaaggtagtaccaatccagaaaaggaagctgaaagtaatgctgtacttgaggaatttcaagaagaaactgagaacgaagatgtcaagcaagaagatgatactgaagctttgctaaagatgctcggtattgatgttgatgttggggctactggtgaggttaaagacgcttcaacctgggttagatggtcagaagaagaaaaatcaagagattgtgatcaatggatgcctgttcgtgcaaaatctgaagctgttgcctctaataatggtgggacacctgatagagaagcagaagaacacctttcgagagtcataatgtatgaagatgttgctgagtacctcttttctttgagctcatttgaggcccgtttatctctagtgttacagttcgttgatttctttggtgggaagacatctcagtggatatctacaaacagttcagcttggtctgagaaactccttagcctagaggcattgccacattcagtattacacactctgaaaagggttcatgatgtcttaagcaaaacacaaggtagttcaaatagtttcagcttggaatctctgttggggaccaccaatgacatccatggacaggcagacctgatgaagtttctttgtaatgctaccttgctctgtttatctgcttttccacgtaattatctcctagaggaagctgctttagttgcagaagagctatctgttgtgaatctgaatccatcccgcagttcagttaccccctgccgtgctctagcaaagcatcttttaaagagtgatcgtcaggatatattgctatgtggtgtttatgcaagaagagaggctttttatgggaatattgatcatgcaagaagagtatttgacatggcgttgtcatctattgaagggcttcctttggaattgaggtccaatgctcctcttctgtatttctggtatgctgaggtggagctagccaaagatcagggaaatcggtctgaatcatcatttcgtgcaatgcatattttatcttgctttggaagtggtggatcatatagtccgtttaaatgtcagccatcaaatttgcaattgctgagagcacgccaaggcttcaaagaaagaataagaacagtgcaaatgtcatgggtacgtggtgttatagatgatcaatccgctgctctcgtatgttgtgctgctttatttgaagagttaacttctggatgggctttgggaattgaagttctagatcaggcttttgcaatggtgcttccagagagaaaaagccatagccatcaacttgaatttgtgttcaacttttacatgaagatgctgcggagacatcatggtcaatcaagtctatcaaaatgctgggagtccattttgcaggggcttcaaatatattcattcagccctgaactttatagtgatctaattgaggttggccatttttatacaacgtccaataaactgcggtgggtgtttgatgactactgtcagaagaaaccatctgttgttgtctggctttttgccttgtcatttgagataagcaaagggggctcacagcataggattcgtggattgtttgaaagggcattggcaaatgataggtttcataactcggttgtgctgtggcgatgctacattgcatatgaaatgaacatagcatgcaatccttccatatccaggcgaattttcttccgagctatccatgcctgcccatggtcaaagaagctatggctggatggttttctcaaattgaactccaccctatctgcaaaagagctgtcagatctccaagaagttatgcgggataaggaactgaatctgagaacagacatttatgaaattcttttgcaagatgagctagtactgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NRDE-2

Protein Analysis

1151

Amino Acids

130.84

Weight (kDa)

5.41

Isoelectric Point (pI)

51.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NRDE-2 PF08424 275 - 672 2.3e-76 NRDE-2, necessary for RNA interference
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010115)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17712 AT3G17712 AT3G17712 AT3G17740
fragaria_vesca FvH4_5g17940 FvH4_5g17940 FvH4_5g17940
malus_domestica MD14G1242200.v1.1
prunus_persica Prupe.5G239800_v2.0.a1
pyrus_communis pycom14g20360
rosa_chinensis RchiOBHm_Chr7g0177661
rosa_laevigata RLG00000005516
rosa_multiflora Rmu_sc0004449.1_g000002 Rmu_ssc0000236.1_g000002
rosa_roxburghii Rroxscaffold_3G00275680
rosa_rugosa Rorug06G0408300
rosa_samantha Rh7AG007800 Rh7BG007700 Rh7CG008200 Rh7DG007900
rosa_wichuraiana Rw7G000650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1035
AasI GACNNNNNNGTC 3 cut(s) 388, 1585, 2925
Acc36I ACCTGC 2 cut(s) 1225, 1324
AccB7I CCANNNNNTGG 1 cut(s) 1351
AccBSI CCGCTC 1 cut(s) 1361
AccI GTMKAC 1 cut(s) 183
AccII CGCG 1 cut(s) 308
AclWI GGATC 4 cut(s) 228, 241, 1259, 2638
AcoI YGGCCR 2 cut(s) 1353, 3022
AcsI RAATTY 7 cut(s) 508, 1382, 1664, 2662, 2882, 3270, 3426
AcuI CTGAAG 2 cut(s) 1734, 1875
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 9 cut(s) 189, 209, 623, 1184, 1621, 1656, 1946, 2730, 3450
AfiI CCNNNNNNNGG 8 cut(s) 339, 360, 904, 929, 1351, 2203, 3264, 3300
AflII CTTAAG 1 cut(s) 2128
AhdI GACNNNNNGTC 2 cut(s) 16, 322
AjnI CCWGG 4 cut(s) 1151, 1197, 1784, 3263
AjuI GAANNNNNNNTTGG 8 cut(s) 77, 109, 863, 895, 1320, 1352, 1473, 1505
AloI GAACNNNNNNTCC 2 cut(s) 2805, 2837
Alw21I GWGCWC 5 cut(s) 937, 1446, 1964, 2359, 2683
Alw26I GTCTC 8 cut(s) 64, 249, 309, 374, 452, 672, 1038, 2911
AlwI GGATC 4 cut(s) 228, 241, 1259, 2638
AlwNI CAGNNNCTG 1 cut(s) 949
Ama87I CYCGRG 2 cut(s) 772, 828
AoxI GGCC 7 cut(s) 14, 191, 369, 435, 1353, 1971, 3022
ApeKI GCWGC 8 cut(s) 485, 755, 919, 1211, 2285, 2756, 2774, 2911
ApoI RAATTY 7 cut(s) 508, 1382, 1664, 2662, 2882, 3270, 3426
ArsI GACNNNNNNTTYG 2 cut(s) 3350, 3382
AseI ATTAAT 1 cut(s) 1061
Asp700I GAANNNNTTC 1 cut(s) 2493
AspLEI GCGC 4 cut(s) 308, 310, 657, 1487
AspS9I GGNCC 6 cut(s) 192, 369, 1497, 1972, 2181, 2511
AsuC2I CCSGG 2 cut(s) 505, 598
AsuHPI GGTGA 4 cut(s) 88, 713, 755, 1775
AsuNHI GCTAGC 1 cut(s) 2554
AvaI CYCGRG 2 cut(s) 772, 828
AvaII GGWCC 3 cut(s) 1497, 2181, 2511
BalI TGGCCA 2 cut(s) 1355, 3024
BamHI GGATCC 1 cut(s) 233
BanII GRGCYC 2 cut(s) 1964, 3140
BbsI GAAGAC 2 cut(s) 1013, 2024
Bbv12I GWGCWC 5 cut(s) 937, 1446, 1964, 2359, 2683
BbvCI CCTCAGC 1 cut(s) 2544
BbvI GCAGC 8 cut(s) 472, 742, 931, 1198, 2272, 2743, 2761, 2898
BceAI ACGGC 6 cut(s) 7, 110, 190, 206, 334, 2337
BcgI CGANNNNNNTGC 4 cut(s) 110, 144, 3134, 3168
BciT130I CCWGG 4 cut(s) 1153, 1199, 1786, 3265
BciVI GTATCC 1 cut(s) 682
BclI TGATCA 3 cut(s) 1825, 2446, 2746
BcnI CCSGG 2 cut(s) 505, 598
BcoDI GTCTC 8 cut(s) 64, 249, 309, 374, 452, 672, 1038, 2911
BfmI CTRYAG 1 cut(s) 451
BfoI RGCGCY 2 cut(s) 658, 1488
BfrI CTTAAG 1 cut(s) 2128
BfuAI ACCTGC 2 cut(s) 1225, 1324
BfuI GTATCC 1 cut(s) 682
BglII AGATCT 1 cut(s) 3370
BisI GCNGC 9 cut(s) 45, 486, 756, 920, 1212, 2286, 2757, 2775, 2912
BlsI GCNGC 9 cut(s) 46, 487, 757, 921, 1213, 2287, 2758, 2776, 2913
BmcAI AGTACT 1 cut(s) 3450
Bme1390I CCNGG 6 cut(s) 505, 598, 1153, 1199, 1786, 3265
Bme18I GGWCC 3 cut(s) 1497, 2181, 2511
BmeRI GACNNNNNGTC 2 cut(s) 16, 322
BmeT110I CYCGRG 2 cut(s) 772, 828
BmgT120I GGNCC 6 cut(s) 192, 369, 1497, 1972, 2181, 2511
BmiI GGNNCC 3 cut(s) 194, 235, 2182
BmrFI CCNGG 6 cut(s) 505, 598, 1153, 1199, 1786, 3265
BmrI ACTGGG 1 cut(s) 29
BmsI GCATC 8 cut(s) 550, 562, 865, 1719, 1824, 2377, 2898, 3206
BmtI GCTAGC 1 cut(s) 2558
BmuI ACTGGG 1 cut(s) 29
BoxI GACNNNNGTC 1 cut(s) 3072
BpiI GAAGAC 2 cut(s) 1013, 2024
BplI GAGNNNNNCTC 2 cut(s) 755, 787
BpmI CTGGAG 3 cut(s) 765, 1135, 1245
Bpu10I CCTNAGC 3 cut(s) 147, 2069, 2544
BpuEI CTTGAG 1 cut(s) 1679
BpuMI CCSGG 2 cut(s) 505, 598
BsaAI YACGTR 2 cut(s) 2264, 2732
BsaBI GATNNNNATC 1 cut(s) 1824
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 672
BsaJI CCNNGG 6 cut(s) 597, 1243, 1785, 2197, 2686, 3299
Bsc4I CCNNNNNNNGG 8 cut(s) 339, 360, 904, 929, 1351, 2203, 3264, 3300
Bse118I RCCGGY 1 cut(s) 332
Bse1I ACTGG 5 cut(s) 35, 354, 414, 782, 1765
Bse3DI GCAATG 5 cut(s) 462, 2081, 2604, 2847, 3222
Bse8I GATNNNNATC 1 cut(s) 1824
BseBI CCWGG 4 cut(s) 1153, 1199, 1786, 3265
BseDI CCNNGG 6 cut(s) 597, 1243, 1785, 2197, 2686, 3299
BseJI GATNNNNATC 1 cut(s) 1824
BseLI CCNNNNNNNGG 8 cut(s) 339, 360, 904, 929, 1351, 2203, 3264, 3300
BseMI GCAATG 5 cut(s) 462, 2081, 2604, 2847, 3222
BseNI ACTGG 5 cut(s) 35, 354, 414, 782, 1765
BsePI GCGCGC 1 cut(s) 306
BseRI GAGGAG 5 cut(s) 188, 301, 1400, 1562, 2511
BseXI GCAGC 8 cut(s) 472, 742, 931, 1198, 2272, 2743, 2761, 2898
BseYI CCCAGC 2 cut(s) 195, 2949
Bsh1236I CGCG 1 cut(s) 308
BshFI GGCC 7 cut(s) 16, 193, 371, 437, 1355, 1973, 3024
BsiHKAI GWGCWC 5 cut(s) 937, 1446, 1964, 2359, 2683
BsiHKCI CYCGRG 2 cut(s) 772, 828
BsiSI CCGG 4 cut(s) 170, 333, 504, 597
BsiWI CGTACG 2 cut(s) 187, 207
BslFI GGGAC 3 cut(s) 53, 1894, 2194
BslI CCNNNNNNNGG 8 cut(s) 339, 360, 904, 929, 1351, 2203, 3264, 3300
BsmAI GTCTC 8 cut(s) 64, 249, 309, 374, 452, 672, 1038, 2911
BsmBI CGTCTC 1 cut(s) 309
BsmFI GGGAC 3 cut(s) 53, 1894, 2194
BsmI GAATGC 2 cut(s) 1093, 1227
BsnI GGCC 7 cut(s) 16, 193, 371, 437, 1355, 1973, 3024
Bso31I GGTCTC 1 cut(s) 672
BsoBI CYCGRG 2 cut(s) 772, 828
Bsp1286I GDGCHC 6 cut(s) 937, 1446, 1964, 2359, 2683, 3140
Bsp19I CCATGG 2 cut(s) 2197, 3299
BspANI GGCC 7 cut(s) 16, 193, 371, 437, 1355, 1973, 3024
BspFNI CGCG 1 cut(s) 308
BspHI TCATGA 2 cut(s) 862, 2119
BspLI GGNNCC 3 cut(s) 194, 235, 2182
BspMI ACCTGC 2 cut(s) 1225, 1324
BspOI GCTAGC 1 cut(s) 2558
BspPI GGATC 4 cut(s) 228, 241, 1259, 2638
BspQI GCTCTTC 1 cut(s) 2295
BspTI CTTAAG 1 cut(s) 2128
BspTNI GGTCTC 1 cut(s) 672
BsrBI CCGCTC 1 cut(s) 1361
BsrDI GCAATG 5 cut(s) 462, 2081, 2604, 2847, 3222
BsrFI RCCGGY 1 cut(s) 332
BsrI ACTGG 5 cut(s) 35, 354, 414, 782, 1765
BssAI RCCGGY 1 cut(s) 332
BssECI CCNNGG 6 cut(s) 597, 1243, 1785, 2197, 2686, 3299
BssHII GCGCGC 1 cut(s) 306
BssNI GRCGYC 1 cut(s) 123
BssT1I CCWWGG 4 cut(s) 1243, 2197, 2686, 3299
Bst2UI CCWGG 4 cut(s) 1153, 1199, 1786, 3265
Bst6I CTCTTC 7 cut(s) 278, 290, 2295, 2416, 2452, 2529, 2781
BstACI GRCGYC 1 cut(s) 123
BstAFI CTTAAG 1 cut(s) 2128
BstBAI YACGTR 2 cut(s) 2264, 2732
BstC8I GCNNGC 9 cut(s) 244, 308, 937, 941, 1216, 2556, 2683, 3247, 3295
BstDSI CCRYGG 2 cut(s) 2197, 3299
BstFNI CGCG 1 cut(s) 308
BstH2I RGCGCY 2 cut(s) 658, 1488
BstHHI GCGC 4 cut(s) 308, 310, 657, 1487
BstMAI GTCTC 8 cut(s) 64, 249, 309, 374, 452, 672, 1038, 2911
BstNI CCWGG 4 cut(s) 1153, 1199, 1786, 3265
BstNSI RCATGY 4 cut(s) 1133, 1193, 1218, 3249
BstPAI GACNNNNGTC 1 cut(s) 3072
BstSCI CCNGG 6 cut(s) 503, 596, 1151, 1197, 1784, 3263
BstSFI CTRYAG 1 cut(s) 451
BstUI CGCG 1 cut(s) 308
BstV1I GCAGC 8 cut(s) 472, 742, 931, 1198, 2272, 2743, 2761, 2898
BstV2I GAAGAC 2 cut(s) 1013, 2024
BstX2I RGATCY 3 cut(s) 233, 1264, 3370
BstXI CCANNNNNNTGG 1 cut(s) 1507
BstYI RGATCY 3 cut(s) 233, 1264, 3370
BsuI GTATCC 1 cut(s) 682
BsuRI GGCC 7 cut(s) 16, 193, 371, 437, 1355, 1973, 3024
BtgI CCRYGG 2 cut(s) 2197, 3299
BtgZI GCGATG 2 cut(s) 261, 3229
BtsIMutI CAGTG 6 cut(s) 361, 421, 1413, 1462, 2033, 2718
BveI ACCTGC 2 cut(s) 1225, 1324
Cac8I GCNNGC 9 cut(s) 244, 308, 937, 941, 1216, 2556, 2683, 3247, 3295
CaiI CAGNNNCTG 1 cut(s) 949
CciI TCATGA 2 cut(s) 862, 2119
CfoI GCGC 4 cut(s) 308, 310, 657, 1487
Cfr10I RCCGGY 1 cut(s) 332
Cfr13I GGNCC 6 cut(s) 192, 369, 1497, 1972, 2181, 2511
CseI GACGC 2 cut(s) 131, 1784
Csp6I GTAC 9 cut(s) 188, 208, 622, 1183, 1620, 1655, 1945, 2729, 3449
CviQI GTAC 9 cut(s) 188, 208, 622, 1183, 1620, 1655, 1945, 2729, 3449
DraI TTTAAA 3 cut(s) 514, 2376, 2647
DrdI GACNNNNNNGTC 3 cut(s) 388, 1585, 2925
DriI GACNNNNNGTC 2 cut(s) 16, 322
DseDI GACNNNNNNGTC 3 cut(s) 388, 1585, 2925
EaeI YGGCCR 2 cut(s) 1353, 3022
Eam1104I CTCTTC 7 cut(s) 278, 290, 2295, 2416, 2452, 2529, 2781
Eam1105I GACNNNNNGTC 2 cut(s) 16, 322
EarI CTCTTC 7 cut(s) 278, 290, 2295, 2416, 2452, 2529, 2781
EciI GGCGGA 1 cut(s) 316
Ecl136II GAGCTC 1 cut(s) 1962
Eco130I CCWWGG 4 cut(s) 1243, 2197, 2686, 3299
Eco24I GRGCYC 2 cut(s) 1964, 3140
Eco31I GGTCTC 1 cut(s) 672
Eco32I GATATC 1 cut(s) 2034
Eco47I GGWCC 3 cut(s) 1497, 2181, 2511
Eco53kI GAGCTC 1 cut(s) 1962
Eco57I CTGAAG 2 cut(s) 1734, 1875
Eco88I CYCGRG 2 cut(s) 772, 828
EcoICRI GAGCTC 1 cut(s) 1962
EcoRI GAATTC 1 cut(s) 1382
EcoRII CCWGG 4 cut(s) 1151, 1197, 1784, 3263
EcoRV GATATC 1 cut(s) 2034
EcoT14I CCWWGG 4 cut(s) 1243, 2197, 2686, 3299
EcoT22I ATGCAT 2 cut(s) 577, 2604
EcoT38I GRGCYC 2 cut(s) 1964, 3140
ErhI CCWWGG 4 cut(s) 1243, 2197, 2686, 3299
Esp3I CGTCTC 1 cut(s) 309
FalI AAGNNNNNCTT 6 cut(s) 1473, 1505, 1893, 1925, 2413, 2445
FaqI GGGAC 3 cut(s) 53, 1894, 2194
FauI CCCGC 4 cut(s) 314, 1276, 2339, 3381
FauNDI CATATG 1 cut(s) 3229
FbaI TGATCA 3 cut(s) 1825, 2446, 2746
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 9 cut(s) 45, 486, 756, 920, 1212, 2286, 2757, 2775, 2912
FriOI GRGCYC 2 cut(s) 1964, 3140
Fsp4HI GCNGC 9 cut(s) 45, 486, 756, 920, 1212, 2286, 2757, 2775, 2912
GlaI GCGC 4 cut(s) 307, 309, 656, 1486
GluI GCNGC 9 cut(s) 45, 486, 756, 920, 1212, 2286, 2757, 2775, 2912
GsaI CCCAGC 2 cut(s) 199, 2953
GsuI CTGGAG 3 cut(s) 765, 1135, 1245
HaeII RGCGCY 2 cut(s) 658, 1488
HaeIII GGCC 7 cut(s) 16, 193, 371, 437, 1355, 1973, 3024
HapII CCGG 4 cut(s) 170, 333, 504, 597
HgaI GACGC 2 cut(s) 131, 1784
HhaI GCGC 4 cut(s) 308, 310, 657, 1487
Hin1I GRCGYC 1 cut(s) 123
Hin6I GCGC 4 cut(s) 306, 308, 655, 1485
HinP1I GCGC 4 cut(s) 306, 308, 655, 1485
HincII GTYRAC 2 cut(s) 184, 2793
HindII GTYRAC 2 cut(s) 184, 2793
HindIII AAGCTT 2 cut(s) 516, 1716
HpaI GTTAAC 1 cut(s) 2793
HpaII CCGG 4 cut(s) 170, 333, 504, 597
HphI GGTGA 4 cut(s) 88, 713, 755, 1775
Hpy166II GTNNAC 5 cut(s) 184, 587, 665, 1500, 2793
Hpy8I GTNNAC 5 cut(s) 184, 587, 665, 1500, 2793
Hpy99I CGWCG 6 cut(s) 11, 22, 28, 125, 188, 394
HpyCH4IV ACGT 5 cut(s) 66, 186, 2263, 2731, 3038
HpySE526I ACGT 5 cut(s) 66, 186, 2263, 2731, 3038
Hsp92I GRCGYC 1 cut(s) 123
HspAI GCGC 4 cut(s) 306, 308, 655, 1485
Ksp22I TGATCA 3 cut(s) 1825, 2446, 2746
KspAI GTTAAC 1 cut(s) 2793
LguI GCTCTTC 1 cut(s) 2295
LmnI GCTCC 7 cut(s) 175, 303, 506, 853, 1413, 2524, 2551
Lsp1109I GCAGC 8 cut(s) 472, 742, 931, 1198, 2272, 2743, 2761, 2898
LweI GCATC 8 cut(s) 550, 562, 865, 1719, 1824, 2377, 2898, 3206
MaeII ACGT 5 cut(s) 66, 186, 2263, 2731, 3038
MaeIII GTNAC 3 cut(s) 765, 1989, 2341
MbiI CCGCTC 1 cut(s) 1361
MfeI CAATTG 1 cut(s) 2669
MflI RGATCY 3 cut(s) 233, 1264, 3370
MhlI GDGCHC 6 cut(s) 937, 1446, 1964, 2359, 2683, 3140
MlsI TGGCCA 2 cut(s) 1355, 3024
MluNI TGGCCA 2 cut(s) 1355, 3024
MlyI GAGTC 5 cut(s) 773, 820, 940, 1926, 2963
MmeI TCCRAC 5 cut(s) 108, 372, 1453, 1488, 1556
Mox20I TGGCCA 2 cut(s) 1355, 3024
Mph1103I ATGCAT 2 cut(s) 577, 2604
MroXI GAANNNNTTC 1 cut(s) 2493
MscI TGGCCA 2 cut(s) 1355, 3024
MslI CAYNNNNRTG 4 cut(s) 861, 1563, 2026, 2906
Msp20I TGGCCA 2 cut(s) 1355, 3024
MspA1I CMGCKG 1 cut(s) 2756
MspCI CTTAAG 1 cut(s) 2128
MspI CCGG 4 cut(s) 170, 333, 504, 597
MspR9I CCNGG 6 cut(s) 505, 598, 1153, 1199, 1786, 3265
MunI CAATTG 1 cut(s) 2669
Mva1269I GAATGC 2 cut(s) 1093, 1227
MvaI CCWGG 4 cut(s) 1153, 1199, 1786, 3265
MvnI CGCG 1 cut(s) 308
NciI CCSGG 2 cut(s) 505, 598
NcoI CCATGG 2 cut(s) 2197, 3299
NdeI CATATG 1 cut(s) 3229
NheI GCTAGC 1 cut(s) 2554
NlaIV GGNNCC 3 cut(s) 194, 235, 2182
NmuCI GTSAC 1 cut(s) 765
NsiI ATGCAT 2 cut(s) 577, 2604
NspI RCATGY 4 cut(s) 1133, 1193, 1218, 3249
PaeI GCATGC 2 cut(s) 1218, 3249
PagI TCATGA 2 cut(s) 862, 2119
PauI GCGCGC 1 cut(s) 306
PciSI GCTCTTC 1 cut(s) 2295
PctI GAATGC 2 cut(s) 1093, 1227
PdmI GAANNNNTTC 1 cut(s) 2493
Pfl23II CGTACG 2 cut(s) 187, 207
PflMI CCANNNNNTGG 1 cut(s) 1351
PfoI TCCNGGA 1 cut(s) 503
PkrI GCNGC 9 cut(s) 46, 487, 757, 921, 1213, 2287, 2758, 2776, 2913
PleI GAGTC 5 cut(s) 772, 820, 940, 1925, 2962
PpsI GAGTC 5 cut(s) 772, 820, 940, 1925, 2962
Ppu21I YACGTR 2 cut(s) 2264, 2732
PshAI GACNNNNGTC 1 cut(s) 3072
PshBI ATTAAT 1 cut(s) 1061
PsiI TTATAA 1 cut(s) 1035
Psp124BI GAGCTC 1 cut(s) 1964
Psp6I CCWGG 4 cut(s) 1151, 1197, 1784, 3263
PspFI CCCAGC 2 cut(s) 195, 2949
PspGI CCWGG 4 cut(s) 1151, 1197, 1784, 3263
PspLI CGTACG 2 cut(s) 187, 207
PspN4I GGNNCC 3 cut(s) 194, 235, 2182
PspPI GGNCC 6 cut(s) 192, 369, 1497, 1972, 2181, 2511
PstNI CAGNNNCTG 1 cut(s) 949
PsuI RGATCY 3 cut(s) 233, 1264, 3370
PteI GCGCGC 1 cut(s) 306
RsaI GTAC 9 cut(s) 189, 209, 623, 1184, 1621, 1656, 1946, 2730, 3450
RsaNI GTAC 9 cut(s) 188, 208, 622, 1183, 1620, 1655, 1945, 2729, 3449
RseI CAYNNNNRTG 4 cut(s) 861, 1563, 2026, 2906
SacI GAGCTC 1 cut(s) 1964
SalI GTCGAC 1 cut(s) 182
SapI GCTCTTC 1 cut(s) 2295
SatI GCNGC 9 cut(s) 45, 486, 756, 920, 1212, 2286, 2757, 2775, 2912
Sau96I GGNCC 6 cut(s) 192, 369, 1497, 1972, 2181, 2511
ScaI AGTACT 1 cut(s) 3450
SchI GAGTC 5 cut(s) 773, 820, 940, 1926, 2963
ScrFI CCNGG 6 cut(s) 505, 598, 1153, 1199, 1786, 3265
SduI GDGCHC 6 cut(s) 937, 1446, 1964, 2359, 2683, 3140
SfaNI GCATC 8 cut(s) 550, 562, 865, 1719, 1824, 2377, 2898, 3206
SfcI CTRYAG 1 cut(s) 451
SinI GGWCC 3 cut(s) 1497, 2181, 2511
SmiMI CAYNNNNRTG 4 cut(s) 861, 1563, 2026, 2906
SmlI CTYRAG 2 cut(s) 1658, 2128
SmoI CTYRAG 2 cut(s) 1658, 2128
SphI GCATGC 2 cut(s) 1218, 3249
SspI AATATT 1 cut(s) 2443
SstI GAGCTC 1 cut(s) 1964
StyD4I CCNGG 6 cut(s) 503, 596, 1151, 1197, 1784, 3263
StyI CCWWGG 4 cut(s) 1243, 2197, 2686, 3299
TaiI ACGT 5 cut(s) 69, 189, 2266, 2734, 3041
TaqI TCGA 5 cut(s) 183, 344, 469, 792, 1913
TaqII GACCGA 1 cut(s) 2565
TatI WGTACW 3 cut(s) 621, 1654, 3448
TauI GCSGC 1 cut(s) 47
TscAI CASTG 6 cut(s) 361, 421, 1420, 1462, 2033, 2718
TseFI GTSAC 1 cut(s) 765
TseI GCWGC 8 cut(s) 485, 755, 919, 1211, 2285, 2756, 2774, 2911
Tsp45I GTSAC 1 cut(s) 765
TspGWI ACGGA 3 cut(s) 100, 734, 2631
TspRI CASTG 6 cut(s) 361, 421, 1420, 1462, 2033, 2718
Van91I CCANNNNNTGG 1 cut(s) 1351
Vha464I CTTAAG 1 cut(s) 2128
VpaK11BI GGWCC 3 cut(s) 1497, 2181, 2511
VspI ATTAAT 1 cut(s) 1061
XapI RAATTY 7 cut(s) 508, 1382, 1664, 2662, 2882, 3270, 3426
XbaI TCTAGA 1 cut(s) 2824
XceI RCATGY 4 cut(s) 1133, 1193, 1218, 3249
XmiI GTMKAC 1 cut(s) 183
XmnI GAANNNNTTC 1 cut(s) 2493
ZrmI AGTACT 1 cut(s) 3450
Zsp2I ATGCAT 2 cut(s) 577, 2604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.