Rmu_ssc0000278.1_g000008

aarF domain-containing protein kinase At4g31390

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000278.1
OG0001871
Physical Location & Seq
Reverse (-)
38434 .. 49497
11064 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4122 bp
atgaattccgtttctgtaaactgcacgcgcacagctccgtttcccgccaagcatctgagctccgccggagctcgtagtgttgccggtcaaataaatggaccgtcggaaggttggagttcgtttgattacaggcgaagagtccggagaagagggaggttccgagtttcgaatgccgcgaccggaacgcggcggagcttggcgtcttcgagcgacggcgagatcggttttgggaagctgagcaagtctagcgccgccatggagcagctcgacttcgagcgcggcgtttgcgtgccgtttcgcaagtatactcccgagactgtgaggaataaggtgttggaatccaatggggcagttgcatcgctaatttcgagaggtgtggagattgtgtggactctgggcttgtattggtctaccttgatgtatgactgcttcgtcggaagggatcaagaagttgttccaaagcgtgctcgacagcttagaaacctcctgtgtgatttggggccttctttcatcaaagctggccaggttcttgcaaacagacctgatattatcagagaagattacatgaatgaactttgcattctccaagatgatgttcctccgttccccaatcaggtcgcgtttgacataatagaagaggagttgggtcaaccccttgaagctgtattcagtaagatttcgccacagacaatagcagctgccagtttgggtcaagtttaccgagctactttacgtgacactggcgaggatgtagctattaaggtacaaaggccagagatagaacctataatttatagggatctttttctgttccgtactcttgcttcattcttaaatggaatcagtctacagaaacttgggtgcaatgctgagctcattgttgatgaatttggtgagaagcttttggaggagcttgattacactttggaagcccgcaatattgaagattttcttgagaactttaaagatgatcctactgtcaagatacctgacgtttacaaacagctttctggtccacgtgttctagtaatggagtggatcgatggtgttcggtgcactaaccctcaggcaattagagaagctggtattgatgtaaatgggttcttaactgttggagtcagtgctgcgttgcgccaattactggaatttggattgttccatggagatccacatcctggaaatgtttttgctatgcgtgatggacgtattggatatgtggactttgggaatgttgctgtacttagtcagcaaaacaaacagattttgatcgatgctgtcgtccatgctgtaaatgaggactatgttgagatggcaaatgatttcaccagactgggcttcttggctcctgggactgatgtctctcctattgttccagctttagaagcaatctggcagaattctcttggaaaaggactgtctgactttaattttcgaagtgttactgggaagtttaatgaattagtttacaactatcccattcgtattccagagaggttttctcttgttatcagatctttattgactcaagagggtatctgttttactttgcagccagacttcaaatttcttgaggttgcatatccatatgtggcaaaacgcctcctaacagatccaaacccggctctgcgtgaacgcctcatacaagtactatttaaggatggtgttttccaatggaagcggctagaaaatctcattgttcttgcaaaagaaaatgtgtccatgatgagcagcaatcctgcattgcaagcgaaagaaatgcaagggttaagagagttgcaagttagcaagaaactggacctcactgacaccatcaaagatggagctcgccttttcatttttgacgaaggaatccgtagaaagctcattcttgctttgaccgaagattcaaagcttcacatagaagagctcgtggatgtgtatagactggttgaagatcagatagacataccaacggttgctgtggaagtagcacgagactttccgtcattcgttcgagatgtgatgctttcgtggagcgatttaacaaccctttgtgcatctttcgggatcatgcctcgtaaagtaaactacgggcttgattacgatgatgaagactacgatgcctatgaagattatgattatgaccatgacgacgatttggaaatagaaaataatggcaaagaacctgagttaaagaaggaaactgtaaggcgtgatgtttggcgttgttccatttgtacatatgataatgaagaaggtttgactgcgtgtgacatttgtggggttcttcgtaaacctttgggtagagttggaagcaatattgataagaaaacagctcctttcaagtttgatgttccatccccagatgatgtggtttccaacggattgcgatcctccaaagtgggttcaaaagccagtgttagtgacgtagagtcttcgggagtttcctcgaaagctgctaagaagaatagtgcagttagggcacaatcaagtgctgaatggtcagatagctcatctactgcagcacaaaaagaccaacaagctagattagataagggaatttcatcagggaatagggcatttagccatccccatggaagtagtggcaccacaactatacaatcaaatggtagtttgtcggacagttcatccttgcagccaacaagctctcatatggaagagggcattagttctttagtgattggaggcaagcctgagagtcttactactagctctacgaacatgtcttccgatgctagatctgggcattcgaaaattgctagttctagaggatcgcattcacgagcagactataaacctgaaaaatggatgcttcctgacgaagctgttgatacattgactcaaatgaatcttgcaattgttggacatgttgattcaggaaaatcaacactctcaggtagactgcttcaccttttgggacgggtatctaaaaaagacatgcacaaatatgagaaagaggcaaagtcacagggcaaggggtcgtttgcatatgcttgggcattggatgagagtgcagaagaaagggaaagaggaataactatgaatgtggctgttgcatattttgattccagaaagtatcatgttgttgtgctggattctcctggccataaagactttgttccaaacatgatatctggggcaacacaagctgatgctgcaatacttgttgtagatgcttcgtgtggtgcattcgaagctggtataaagggacagactatggaacatgcccaacttatcagaagctttggtgttgatcaaattatagttgcggttaacaagatggatgttgttgaatactccaaggatcgatttgatgaaattaaacagacacttggaacatttctccattcccgtggcttcaaagattcttcagtgtattgggttccattgagtgtcatggaaaatcaaaatttggtatcagctccgtctgaggctcgtttgttgtcttggtatcatggaccttccctgttggattcaattgattcccttcaacctcccacgagagattttcaaaggccactgctcatgccaatatgtgatgtcattagatctacttctctgggacaggtgtctgcttgtggtaaattggaagcaggagctcttcggagtggatcaaaggttctggttatgccttcaggagaattagggattgtccgctctttagaacgggattctcaggcatgttccattgcaagagctggtgacaatgtgggtgttactcttcaaggcattgatggtggtcatgtgatggcagggggcgtgctctgtcaccctggttttccggttgcagttgcaaaacatttggaattgaaagtgcttcttttggatgtcgccacccctattttaattggctctcaggtggaatttcacatacaccacgccaaggaggctgccacagttgtaaaaatattgtcattaattgacccaaagggaaaagtgacgaaaaagtctcctcgctgtctacttgcaaagcagagggctgttatagaggtagttttgcaaggaccagtttgtgtggaagagtatacaagttctaaagctctagggagcgtatctctgagggcatcaggaaacactgtggccctcggtgttgtaagccggattataaaggagcaggagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006091 GO:0006464 GO:0006468 GO:0006725 GO:0006778 GO:0006787 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0006995 GO:0006996 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009267 GO:0009314 GO:0009414 GO:0009415 GO:0009416 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009628 GO:0009635 GO:0009636 GO:0009637 GO:0009639 GO:0009657 GO:0009658 GO:0009668 GO:0009767 GO:0009889 GO:0009891 GO:0009893 GO:0009962 GO:0009987 GO:0009991 GO:0010027 GO:0010033 GO:0010035 GO:0010109 GO:0010114 GO:0010287 GO:0010380 GO:0015979 GO:0015994 GO:0015996 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019216 GO:0019222 GO:0019439 GO:0019538 GO:0019684 GO:0019747 GO:0022900 GO:0031279 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031537 GO:0031540 GO:0031667 GO:0031668 GO:0031669 GO:0033013 GO:0033015 GO:0033554 GO:0034641 GO:0036211 GO:0042221 GO:0042440 GO:0042594 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043562 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044267 GO:0044270 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045828 GO:0045834 GO:0046149 GO:0046483 GO:0046700 GO:0046889 GO:0046890 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051186 GO:0051187 GO:0051193 GO:0051194 GO:0051716 GO:0055114 GO:0061024 GO:0065007 GO:0065009 GO:0071496 GO:0071704 GO:0071840 GO:0080090 GO:0080177 GO:0080183 GO:0090056 GO:0140096 GO:1901360 GO:1901361 GO:1901401 GO:1901403 GO:1901463 GO:1901465 GO:1901562 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902171 GO:1902326 GO:1904143
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1

Protein Analysis

1373

Amino Acids

150.98

Weight (kDa)

5.77

Isoelectric Point (pI)

42.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 195 - 439 3e-72 ABC1 atypical kinase-like domain
GTP_EFTU PF00009 949 - 1163 1.8e-48 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 1193 - 1256 4.5e-08 Elongation factor Tu domain 2
GTP_EFTU_D3 PF03143 1264 - 1368 4.1e-10 Elongation factor Tu C-terminal domain
GTP-eEF1A_C PF22594 1268 - 1365 7.3e-24 GTP-eEF1A C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4106
AasI GACNNNNNNGTC 2 cut(s) 431, 3946
AccB1I GGYRCC 1 cut(s) 2585
AccB7I CCANNNNNTGG 2 cut(s) 1141, 1175
AccBSI CCGCTC 1 cut(s) 3657
AccI GTMKAC 6 cut(s) 305, 410, 847, 2899, 3961, 4025
AccII CGCG 5 cut(s) 28, 176, 187, 279, 620
AccIII TCCGGA 1 cut(s) 141
AcoI YGGCCR 2 cut(s) 520, 3103
AcsI RAATTY 8 cut(s) 4, 887, 1145, 1396, 1562, 2538, 3409, 3863
AcuI CTGAAG 2 cut(s) 3354, 3618
AcvI CACGTG 1 cut(s) 1019
AcyI GRCGYC 1 cut(s) 200
AfaI GTAC 5 cut(s) 765, 817, 1239, 1647, 2218
AfiI CCNNNNNNNGG 6 cut(s) 107, 186, 613, 1141, 1175, 3883
AflIII ACRYGT 3 cut(s) 1018, 2721, 2866
AhdI GACNNNNNGTC 2 cut(s) 1981, 2410
AjnI CCWGG 5 cut(s) 522, 1174, 1345, 3100, 3772
AjuI GAANNNNNNNTTGG 4 cut(s) 317, 349, 579, 611
AloI GAACNNNNNNTCC 4 cut(s) 2711, 2743, 3603, 3635
Alw21I GWGCWC 9 cut(s) 62, 73, 469, 876, 1058, 1825, 1908, 3601, 3765
Alw26I GTCTC 4 cut(s) 308, 1363, 1968, 3954
Alw44I GTGCAC 1 cut(s) 1054
Ama87I CYCGRG 1 cut(s) 311
Aor13HI TCCGGA 1 cut(s) 141
AoxI GGCC 6 cut(s) 500, 520, 770, 3103, 3515, 4080
ApaLI GTGCAC 1 cut(s) 1054
ApoI RAATTY 8 cut(s) 4, 887, 1145, 1396, 1562, 2538, 3409, 3863
AseI ATTAAT 1 cut(s) 3917
Asp700I GAANNNNTTC 2 cut(s) 453, 948
AspLEI GCGC 4 cut(s) 30, 251, 279, 1134
AspS9I GGNCC 7 cut(s) 98, 500, 1013, 1795, 3458, 4004, 4081
AsuC2I CCSGG 1 cut(s) 1619
AsuHPI GGTGA 5 cut(s) 905, 1315, 2900, 3713, 3761
AsuII TTCGAA 4 cut(s) 167, 1432, 2750, 3192
AvaI CYCGRG 1 cut(s) 311
AvaII GGWCC 5 cut(s) 98, 1013, 1795, 3458, 4004
AxyI CCTNAGG 1 cut(s) 1065
BaeGI GKGCMC 2 cut(s) 1058, 2464
BalI TGGCCA 2 cut(s) 522, 3105
BanI GGYRCC 1 cut(s) 2585
BanII GRGCYC 6 cut(s) 62, 73, 876, 1825, 1908, 3601
BarI GAAGNNNNNNTAC 4 cut(s) 413, 445, 808, 840
BauI CACGAG 4 cut(s) 1907, 1971, 2781, 3499
BbrPI CACGTG 1 cut(s) 1019
BbsI GAAGAC 4 cut(s) 195, 2097, 2406, 2718
Bbv12I GWGCWC 9 cut(s) 62, 73, 469, 876, 1058, 1825, 1908, 3601, 3765
BceAI ACGGC 2 cut(s) 229, 277
BcgI CGANNNNNNTGC 4 cut(s) 339, 373, 1738, 1772
BciT130I CCWGG 5 cut(s) 524, 1176, 1347, 3102, 3774
BclI TGATCA 1 cut(s) 3253
BcnI CCSGG 1 cut(s) 1619
BcoDI GTCTC 4 cut(s) 308, 1363, 1968, 3954
BfaI CTAG 9 cut(s) 246, 1025, 1682, 2523, 2709, 2736, 2760, 2766, 4043
BfmI CTRYAG 2 cut(s) 848, 2499
BfoI RGCGCY 1 cut(s) 252
BglI GCCNNNNNGGC 1 cut(s) 3887
BglII AGATCT 3 cut(s) 1511, 2738, 3548
BlpI GCTNAGC 2 cut(s) 236, 870
BmcAI AGTACT 1 cut(s) 1647
Bme1390I CCNGG 6 cut(s) 524, 1176, 1347, 1619, 3102, 3774
Bme18I GGWCC 5 cut(s) 98, 1013, 1795, 3458, 4004
BmeRI GACNNNNNGTC 2 cut(s) 1981, 2410
BmeT110I CYCGRG 1 cut(s) 311
BmgT120I GGNCC 7 cut(s) 98, 500, 1013, 1795, 3458, 4004, 4081
BmiI GGNNCC 5 cut(s) 158, 501, 1344, 2587, 3384
BmrFI CCNGG 6 cut(s) 524, 1176, 1347, 1619, 3102, 3774
BmrI ACTGGG 2 cut(s) 1340, 1452
BmuI ACTGGG 2 cut(s) 1340, 1452
BoxI GACNNNNGTC 2 cut(s) 1355, 3568
BpiI GAAGAC 4 cut(s) 195, 2097, 2406, 2718
BplI GAGNNNNNCTC 4 cut(s) 1483, 1515, 4039, 4071
Bpu1102I GCTNAGC 2 cut(s) 236, 870
Bpu14I TTCGAA 4 cut(s) 167, 1432, 2750, 3192
BpuEI CTTGAG 3 cut(s) 974, 1509, 1589
BpuMI CCSGG 1 cut(s) 1619
Bsa29I ATCGAT 3 cut(s) 1041, 1269, 3307
BsaAI YACGTR 2 cut(s) 734, 1019
BsaBI GATNNNNATC 3 cut(s) 1170, 1265, 2368
BsaHI GRCGYC 1 cut(s) 200
BsaJI CCNNGG 9 cut(s) 255, 1159, 1346, 2572, 3300, 3352, 3772, 3882, 4084
BsaWI WCCGGW 3 cut(s) 141, 179, 3781
BsaXI ACNNNNNCTCC 3 cut(s) 371, 401, 4103
Bsc4I CCNNNNNNNGG 6 cut(s) 107, 186, 613, 1141, 1175, 3883
Bse118I RCCGGY 1 cut(s) 83
Bse1I ACTGG 9 cut(s) 702, 745, 1146, 1335, 1447, 1797, 1929, 2394, 4007
Bse21I CCTNAGG 1 cut(s) 1065
Bse3DI GCAATG 3 cut(s) 871, 1739, 3687
Bse8I GATNNNNATC 3 cut(s) 1170, 1265, 2368
BseAI TCCGGA 1 cut(s) 141
BseBI CCWGG 5 cut(s) 524, 1176, 1347, 3102, 3774
BseCI ATCGAT 3 cut(s) 1041, 1269, 3307
BseDI CCNNGG 9 cut(s) 255, 1159, 1346, 2572, 3300, 3352, 3772, 3882, 4084
BseJI GATNNNNATC 3 cut(s) 1170, 1265, 2368
BseLI CCNNNNNNNGG 6 cut(s) 107, 186, 613, 1141, 1175, 3883
BseMI GCAATG 3 cut(s) 871, 1739, 3687
BseNI ACTGG 9 cut(s) 702, 745, 1146, 1335, 1447, 1797, 1929, 2394, 4007
BseRI GAGGAG 3 cut(s) 653, 923, 3942
BseSI GKGCMC 2 cut(s) 1058, 2464
BsgI GTGCAG 3 cut(s) 7, 2472, 3033
Bsh1236I CGCG 5 cut(s) 28, 176, 187, 279, 620
Bsh1285I CGRYCG 1 cut(s) 180
BshFI GGCC 6 cut(s) 502, 522, 772, 3105, 3517, 4082
BshNI GGYRCC 1 cut(s) 2585
BshVI ATCGAT 3 cut(s) 1041, 1269, 3307
BsiEI CGRYCG 1 cut(s) 180
BsiHKAI GWGCWC 9 cut(s) 62, 73, 469, 876, 1058, 1825, 1908, 3601, 3765
BsiHKCI CYCGRG 1 cut(s) 311
BsiSI CCGG 7 cut(s) 66, 84, 142, 180, 1619, 3782, 4099
BslFI GGGAC 4 cut(s) 1363, 2931, 3222, 3576
BslI CCNNNNNNNGG 6 cut(s) 107, 186, 613, 1141, 1175, 3883
BsmAI GTCTC 4 cut(s) 308, 1363, 1968, 3954
BsmFI GGGAC 4 cut(s) 1363, 2931, 3222, 3576
BsmI GAATGC 5 cut(s) 175, 579, 2746, 2776, 3188
BsnI GGCC 6 cut(s) 502, 522, 772, 3105, 3517, 4082
BsoBI CYCGRG 1 cut(s) 311
Bsp119I TTCGAA 4 cut(s) 167, 1432, 2750, 3192
Bsp13I TCCGGA 1 cut(s) 141
Bsp1407I TGTACA 1 cut(s) 2216
Bsp1720I GCTNAGC 2 cut(s) 236, 870
Bsp19I CCATGG 3 cut(s) 255, 1159, 2572
BspANI GGCC 6 cut(s) 502, 522, 772, 3105, 3517, 4082
BspDI ATCGAT 3 cut(s) 1041, 1269, 3307
BspEI TCCGGA 1 cut(s) 141
BspFNI CGCG 5 cut(s) 28, 176, 187, 279, 620
BspLI GGNNCC 5 cut(s) 158, 501, 1344, 2587, 3384
BspMAI CTGCAG 1 cut(s) 2503
BspQI GCTCTTC 2 cut(s) 1896, 3606
BspT104I TTCGAA 4 cut(s) 167, 1432, 2750, 3192
BspT107I GGYRCC 1 cut(s) 2585
BsrBI CCGCTC 1 cut(s) 3657
BsrDI GCAATG 3 cut(s) 871, 1739, 3687
BsrFI RCCGGY 1 cut(s) 83
BsrGI TGTACA 1 cut(s) 2216
BsrI ACTGG 9 cut(s) 702, 745, 1146, 1335, 1447, 1797, 1929, 2394, 4007
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 9 cut(s) 255, 1159, 1346, 2572, 3300, 3352, 3772, 3882, 4084
BssNAI GTATAC 2 cut(s) 306, 4026
BssNI GRCGYC 1 cut(s) 200
BssSI CACGAG 4 cut(s) 1907, 1971, 2781, 3499
BssT1I CCWWGG 5 cut(s) 255, 1159, 2572, 3300, 3882
Bst1107I GTATAC 2 cut(s) 306, 4026
Bst2BI CACGAG 4 cut(s) 1907, 1971, 2781, 3499
Bst2UI CCWGG 5 cut(s) 524, 1176, 1347, 3102, 3774
Bst6I CTCTTC 8 cut(s) 130, 142, 630, 1896, 2652, 3606, 3726, 4014
BstACI GRCGYC 1 cut(s) 200
BstAUI TGTACA 1 cut(s) 2216
BstBAI YACGTR 2 cut(s) 734, 1019
BstBI TTCGAA 4 cut(s) 167, 1432, 2750, 3192
BstC8I GCNNGC 9 cut(s) 26, 290, 465, 520, 934, 1746, 1825, 2690, 3761
BstDSI CCRYGG 4 cut(s) 255, 1159, 2572, 3352
BstFNI CGCG 5 cut(s) 28, 176, 187, 279, 620
BstH2I RGCGCY 1 cut(s) 252
BstHHI GCGC 4 cut(s) 30, 251, 279, 1134
BstMAI GTCTC 4 cut(s) 308, 1363, 1968, 3954
BstMCI CGRYCG 1 cut(s) 180
BstMWI GCNNNNNNNGC 9 cut(s) 246, 285, 1382, 1745, 2459, 3146, 3155, 3194, 3887
BstNI CCWGG 5 cut(s) 524, 1176, 1347, 3102, 3774
BstNSI RCATGY 5 cut(s) 2725, 2870, 2941, 3227, 3684
BstPAI GACNNNNGTC 2 cut(s) 1355, 3568
BstSCI CCNGG 6 cut(s) 522, 1174, 1345, 1617, 3100, 3772
BstSFI CTRYAG 2 cut(s) 848, 2499
BstSLI GKGCMC 2 cut(s) 1058, 2464
BstUI CGCG 5 cut(s) 28, 176, 187, 279, 620
BstV2I GAAGAC 4 cut(s) 195, 2097, 2406, 2718
BstX2I RGATCY 6 cut(s) 799, 1165, 1511, 1609, 2738, 3548
BstXI CCANNNNNNTGG 2 cut(s) 2573, 3353
BstYI RGATCY 6 cut(s) 799, 1165, 1511, 1609, 2738, 3548
BstZ17I GTATAC 2 cut(s) 306, 4026
Bsu15I ATCGAT 3 cut(s) 1041, 1269, 3307
Bsu36I CCTNAGG 1 cut(s) 1065
BsuRI GGCC 6 cut(s) 502, 522, 772, 3105, 3517, 4082
BsuTUI ATCGAT 3 cut(s) 1041, 1269, 3307
BtgI CCRYGG 4 cut(s) 255, 1159, 2572, 3352
BtgZI GCGATG 1 cut(s) 342
BtsI GCAGTG 1 cut(s) 3518
BtsIMutI CAGTG 7 cut(s) 738, 1126, 1800, 2401, 3378, 3518, 4074
Cac8I GCNNGC 9 cut(s) 26, 290, 465, 520, 934, 1746, 1825, 2690, 3761
CfoI GCGC 4 cut(s) 30, 251, 279, 1134
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 7 cut(s) 98, 500, 1013, 1795, 3458, 4004, 4081
ClaI ATCGAT 3 cut(s) 1041, 1269, 3307
CseI GACGC 1 cut(s) 189
Csp6I GTAC 5 cut(s) 764, 816, 1238, 1646, 2217
CviQI GTAC 5 cut(s) 764, 816, 1238, 1646, 2217
DraI TTTAAA 1 cut(s) 964
DrdI GACNNNNNNGTC 2 cut(s) 431, 3946
DriI GACNNNNNGTC 2 cut(s) 1981, 2410
DseDI GACNNNNNNGTC 2 cut(s) 431, 3946
EaeI YGGCCR 2 cut(s) 520, 3103
Eam1104I CTCTTC 8 cut(s) 130, 142, 630, 1896, 2652, 3606, 3726, 4014
Eam1105I GACNNNNNGTC 2 cut(s) 1981, 2410
EarI CTCTTC 8 cut(s) 130, 142, 630, 1896, 2652, 3606, 3726, 4014
EciI GGCGGA 2 cut(s) 52, 205
Ecl136II GAGCTC 6 cut(s) 60, 71, 874, 1823, 1906, 3599
Eco130I CCWWGG 5 cut(s) 255, 1159, 2572, 3300, 3882
Eco24I GRGCYC 6 cut(s) 62, 73, 876, 1825, 1908, 3601
Eco32I GATATC 1 cut(s) 3132
Eco47I GGWCC 5 cut(s) 98, 1013, 1795, 3458, 4004
Eco53kI GAGCTC 6 cut(s) 60, 71, 874, 1823, 1906, 3599
Eco57I CTGAAG 2 cut(s) 3354, 3618
Eco72I CACGTG 1 cut(s) 1019
Eco81I CCTNAGG 1 cut(s) 1065
Eco88I CYCGRG 1 cut(s) 311
EcoICRI GAGCTC 6 cut(s) 60, 71, 874, 1823, 1906, 3599
EcoO109I RGGNCCY 1 cut(s) 500
EcoRI GAATTC 2 cut(s) 4, 1396
EcoRII CCWGG 5 cut(s) 522, 1174, 1345, 3100, 3772
EcoRV GATATC 1 cut(s) 3132
EcoT14I CCWWGG 5 cut(s) 255, 1159, 2572, 3300, 3882
EcoT38I GRGCYC 6 cut(s) 62, 73, 876, 1825, 1908, 3601
ErhI CCWWGG 5 cut(s) 255, 1159, 2572, 3300, 3882
FalI AAGNNNNNCTT 4 cut(s) 936, 968, 3804, 3836
FaqI GGGAC 4 cut(s) 1363, 2931, 3222, 3576
FauI CCCGC 2 cut(s) 52, 941
FauNDI CATATG 4 cut(s) 1585, 2221, 2652, 2989
FbaI TGATCA 1 cut(s) 3253
FblI GTMKAC 6 cut(s) 305, 410, 847, 2899, 3961, 4025
FriOI GRGCYC 6 cut(s) 62, 73, 876, 1825, 1908, 3601
FspBI CTAG 9 cut(s) 246, 1025, 1682, 2523, 2709, 2736, 2760, 2766, 4043
GlaI GCGC 4 cut(s) 29, 250, 278, 1133
HaeII RGCGCY 1 cut(s) 252
HaeIII GGCC 6 cut(s) 502, 522, 772, 3105, 3517, 4082
HapII CCGG 7 cut(s) 66, 84, 142, 180, 1619, 3782, 4099
HgaI GACGC 1 cut(s) 189
HhaI GCGC 4 cut(s) 30, 251, 279, 1134
Hin1I GRCGYC 1 cut(s) 200
Hin6I GCGC 4 cut(s) 28, 249, 277, 1132
HinP1I GCGC 4 cut(s) 28, 249, 277, 1132
HincII GTYRAC 2 cut(s) 650, 3274
HindII GTYRAC 2 cut(s) 650, 3274
HindIII AAGCTT 3 cut(s) 899, 1889, 3241
HpaI GTTAAC 1 cut(s) 3274
HpaII CCGG 7 cut(s) 66, 84, 142, 180, 1619, 3782, 4099
HphI GGTGA 5 cut(s) 905, 1315, 2900, 3713, 3761
Hpy99I CGWCG 4 cut(s) 106, 215, 437, 2135
HpyAV CCTTC 9 cut(s) 101, 432, 513, 1838, 2170, 2228, 3471, 3497, 3642
HpyCH4IV ACGT 5 cut(s) 733, 993, 1018, 1204, 2406
HpyF10VI GCNNNNNNNGC 9 cut(s) 246, 285, 1382, 1745, 2459, 3146, 3155, 3194, 3887
HpySE526I ACGT 5 cut(s) 733, 993, 1018, 1204, 2406
Hsp92I GRCGYC 1 cut(s) 200
HspAI GCGC 4 cut(s) 28, 249, 277, 1132
Kpn2I TCCGGA 1 cut(s) 141
Ksp22I TGATCA 1 cut(s) 3253
KspAI GTTAAC 1 cut(s) 3274
LguI GCTCTTC 2 cut(s) 1896, 3606
MaeI CTAG 9 cut(s) 246, 1025, 1682, 2523, 2709, 2736, 2760, 2766, 4043
MaeII ACGT 5 cut(s) 733, 993, 1018, 1204, 2406
MaeIII GTNAC 9 cut(s) 734, 1438, 2249, 2402, 2964, 3701, 3715, 3767, 3937
MbiI CCGCTC 1 cut(s) 3657
MfeI CAATTG 2 cut(s) 2856, 3477
MflI RGATCY 6 cut(s) 799, 1165, 1511, 1609, 2738, 3548
MlsI TGGCCA 2 cut(s) 522, 3105
MluNI TGGCCA 2 cut(s) 522, 3105
MlyI GAGTC 7 cut(s) 147, 385, 1125, 1516, 2420, 2707, 2833
Mox20I TGGCCA 2 cut(s) 522, 3105
MroI TCCGGA 1 cut(s) 141
MroXI GAANNNNTTC 2 cut(s) 453, 948
MscI TGGCCA 2 cut(s) 522, 3105
MslI CAYNNNNRTG 3 cut(s) 2571, 2946, 3351
Msp20I TGGCCA 2 cut(s) 522, 3105
MspA1I CMGCKG 1 cut(s) 698
MspI CCGG 7 cut(s) 66, 84, 142, 180, 1619, 3782, 4099
MspR9I CCNGG 6 cut(s) 524, 1176, 1347, 1619, 3102, 3774
MunI CAATTG 2 cut(s) 2856, 3477
Mva1269I GAATGC 5 cut(s) 175, 579, 2746, 2776, 3188
MvaI CCWGG 5 cut(s) 524, 1176, 1347, 3102, 3774
MvnI CGCG 5 cut(s) 28, 176, 187, 279, 620
MwoI GCNNNNNNNGC 9 cut(s) 246, 285, 1382, 1745, 2459, 3146, 3155, 3194, 3887
NciI CCSGG 1 cut(s) 1619
NcoI CCATGG 3 cut(s) 255, 1159, 2572
NdeI CATATG 4 cut(s) 1585, 2221, 2652, 2989
NlaIV GGNNCC 5 cut(s) 158, 501, 1344, 2587, 3384
NmuCI GTSAC 7 cut(s) 734, 2249, 2402, 2964, 3701, 3767, 3937
NspI RCATGY 5 cut(s) 2725, 2870, 2941, 3227, 3684
NspV TTCGAA 4 cut(s) 167, 1432, 2750, 3192
PciI ACATGT 2 cut(s) 2721, 2866
PciSI GCTCTTC 2 cut(s) 1896, 3606
PcsI WCGNNNNNNNCGW 4 cut(s) 173, 279, 365, 1275
PctI GAATGC 5 cut(s) 175, 579, 2746, 2776, 3188
PdmI GAANNNNTTC 2 cut(s) 453, 948
PflMI CCANNNNNTGG 2 cut(s) 1141, 1175
PfoI TCCNGGA 1 cut(s) 1174
PleI GAGTC 7 cut(s) 146, 385, 1124, 1516, 2419, 2706, 2833
PmaCI CACGTG 1 cut(s) 1019
PmlI CACGTG 1 cut(s) 1019
PpsI GAGTC 7 cut(s) 146, 385, 1124, 1516, 2419, 2706, 2833
Ppu21I YACGTR 2 cut(s) 734, 1019
PscI ACATGT 2 cut(s) 2721, 2866
PshAI GACNNNNGTC 2 cut(s) 1355, 3568
PshBI ATTAAT 1 cut(s) 3917
PsiI TTATAA 1 cut(s) 4106
Psp124BI GAGCTC 6 cut(s) 62, 73, 876, 1825, 1908, 3601
Psp6I CCWGG 5 cut(s) 522, 1174, 1345, 3100, 3772
PspCI CACGTG 1 cut(s) 1019
PspGI CCWGG 5 cut(s) 522, 1174, 1345, 3100, 3772
PspN4I GGNNCC 5 cut(s) 158, 501, 1344, 2587, 3384
PspPI GGNCC 7 cut(s) 98, 500, 1013, 1795, 3458, 4004, 4081
PsrI GAACNNNNNNTAC 2 cut(s) 1623, 1655
PstI CTGCAG 1 cut(s) 2503
PsuI RGATCY 6 cut(s) 799, 1165, 1511, 1609, 2738, 3548
PvuII CAGCTG 1 cut(s) 698
RsaI GTAC 5 cut(s) 765, 817, 1239, 1647, 2218
RsaNI GTAC 5 cut(s) 764, 816, 1238, 1646, 2217
RseI CAYNNNNRTG 3 cut(s) 2571, 2946, 3351
SacI GAGCTC 6 cut(s) 62, 73, 876, 1825, 1908, 3601
SapI GCTCTTC 2 cut(s) 1896, 3606
Sau96I GGNCC 7 cut(s) 98, 500, 1013, 1795, 3458, 4004, 4081
ScaI AGTACT 1 cut(s) 1647
SchI GAGTC 7 cut(s) 147, 385, 1125, 1516, 2420, 2707, 2833
ScrFI CCNGG 6 cut(s) 524, 1176, 1347, 1619, 3102, 3774
SfcI CTRYAG 2 cut(s) 848, 2499
SfuI TTCGAA 4 cut(s) 167, 1432, 2750, 3192
SinI GGWCC 5 cut(s) 98, 1013, 1795, 3458, 4004
SmiMI CAYNNNNRTG 3 cut(s) 2571, 2946, 3351
SmlI CTYRAG 3 cut(s) 953, 1524, 1568
SmoI CTYRAG 3 cut(s) 953, 1524, 1568
SspI AATATT 3 cut(s) 940, 2299, 3909
SspMI CTAG 9 cut(s) 246, 1025, 1682, 2523, 2709, 2736, 2760, 2766, 4043
SstI GAGCTC 6 cut(s) 62, 73, 876, 1825, 1908, 3601
StyD4I CCNGG 6 cut(s) 522, 1174, 1345, 1617, 3100, 3772
StyI CCWWGG 5 cut(s) 255, 1159, 2572, 3300, 3882
TaiI ACGT 5 cut(s) 736, 996, 1021, 1207, 2409
TaqII GACCGA 1 cut(s) 1892
TatI WGTACW 3 cut(s) 1237, 1645, 2216
TauI GCSGC 5 cut(s) 176, 190, 254, 282, 1681
TscAI CASTG 7 cut(s) 745, 1126, 1807, 2401, 3378, 3525, 4081
TseFI GTSAC 7 cut(s) 734, 2249, 2402, 2964, 3701, 3767, 3937
Tsp45I GTSAC 7 cut(s) 734, 2249, 2402, 2964, 3701, 3767, 3937
TspGWI ACGGA 7 cut(s) 27, 591, 803, 1841, 1971, 2376, 3414
TspRI CASTG 7 cut(s) 745, 1126, 1807, 2401, 3378, 3525, 4081
Van91I CCANNNNNTGG 2 cut(s) 1141, 1175
VneI GTGCAC 1 cut(s) 1054
VpaK11BI GGWCC 5 cut(s) 98, 1013, 1795, 3458, 4004
VspI ATTAAT 1 cut(s) 3917
XapI RAATTY 8 cut(s) 4, 887, 1145, 1396, 1562, 2538, 3409, 3863
XbaI TCTAGA 1 cut(s) 2765
XceI RCATGY 5 cut(s) 2725, 2870, 2941, 3227, 3684
XcmI CCANNNNNNNNNTGG 1 cut(s) 2579
XmiI GTMKAC 6 cut(s) 305, 410, 847, 2899, 3961, 4025
XmnI GAANNNNTTC 2 cut(s) 453, 948
XspI CTAG 9 cut(s) 246, 1025, 1682, 2523, 2709, 2736, 2760, 2766, 4043
ZrmI AGTACT 1 cut(s) 1647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.