Rmu_ssc0000333.1_g000011

Protein phosphatase 2C and cyclic nucleotide-binding kinase domain-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000333.1
OG0006775
Physical Location & Seq
Reverse (-)
46132 .. 59623
13492 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1281 bp
atgggttgcgtgtattcgagggtctgtattggagaggttagtagtagtagtagttcaagagatgcgagaaggagagaggcccggaatgcgaggaacactgagatcccagttttctcacccaactcttcaagtgaggacgatggtgagactggagatcagttcaaccaggcaaaccataccagagacgctgaaatggggatcactaggctgtcgagggtttcggcacagtttctacctccaaatgggtcgagaactgtcaaggttccctccggcaagtttgagttgaggtactcgtatttgtcccagagagggtattaccctgatgctctcgataaggccaaccaggacagcttttgcatccacactccgtttgggacgaacccgaatgaccattttttcggggtgtttgatggccatggcgagtttggaaccgagtgttcgcagtttgtcaagaggaagttgtgtgagaatttgcttaggcatggtaagtttcaagtggatgctgtcgaggcgtgtcgcgaggcgtttctcgccacgaattcgcagttgcatgatgacattgtggatgacagcatgagtgggacaacagccattacggttttggttagaggtaggaagatatacattgccaattcgggggattctagagctgttatagcggagaggaatggggaggagttggtggcggtggatctttcgattgatcagaccccgttcagggtggatgagctcgaaagggttaagatttgtggtgcaagagtgctcacattggatcagattgaagggctgaagaatcctgatgtgcagtgttggggcaccgaagaaggtgatgatggtgatccgcctaggctgtgggtgccgaatggaatgtatcctgggaccgcttttaccaggagtattggtgattcgattgctgagtcaattggagttgttgccaaccctgaaattgtggttctggagcttacacagaatcatcctttcttcgtccttgctagtgatggagtgtttgagtttatttctagccaaaccgtggttgatatgtctgtgtgcgcagtggttatgtgcgtgggatgccggattgtggcgaagcaagatcatggtgggatgagatcaatgactgcgggtcgaggtggattggattcgcggtggggcgagtttgggatgttgcgggtcgtggaggcttgggttgacgtaactccgtgtagctcgtggtttgggaggcgcaggggcgacgttgatcggagtaacactggtcgtgtacagcgtggtcgggcggcgtaa

Protein Analysis

426

Amino Acids

46.85

Weight (kDa)

5.34

Isoelectric Point (pI)

38.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 122 - 347 1.1e-33 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1051
AccB1I GGYRCC 2 cut(s) 815, 856
AccB7I CCANNNNNTGG 1 cut(s) 1030
AccII CGCG 2 cut(s) 519, 1144
AciI CCGC 8 cut(s) 659, 686, 842, 882, 1121, 1144, 1168, 1274
AclWI GGATC 5 cut(s) 97, 206, 699, 780, 833
AcoI YGGCCR 1 cut(s) 412
AcsI RAATTY 2 cut(s) 469, 538
AcuI CTGAAG 1 cut(s) 809
AfaI GTAC 2 cut(s) 290, 1260
AfiI CCNNNNNNNGG 7 cut(s) 242, 309, 636, 717, 718, 1030, 1081
AgsI TTSAA 5 cut(s) 57, 129, 163, 494, 782
AhdI GACNNNNNGTC 1 cut(s) 1122
AjnI CCWGG 4 cut(s) 165, 342, 874, 890
AluBI AGCT 5 cut(s) 351, 650, 730, 961, 1206
AluI AGCT 5 cut(s) 351, 650, 730, 961, 1206
Alw21I GWGCWC 2 cut(s) 732, 765
Alw26I GTCTC 2 cut(s) 140, 177
AlwI GGATC 5 cut(s) 97, 206, 699, 780, 833
AoxI GGCC 3 cut(s) 78, 336, 412
ApoI RAATTY 2 cut(s) 469, 538
ArsI GACNNNNNNTTYG 2 cut(s) 380, 412
AspA2I CCTAGG 1 cut(s) 845
AspLEI GCGC 2 cut(s) 1052, 1224
AspS9I GGNCC 2 cut(s) 79, 879
AsuC2I CCSGG 1 cut(s) 82
AsuHPI GGTGA 5 cut(s) 108, 155, 839, 848, 914
AvaII GGWCC 1 cut(s) 879
AvrII CCTAGG 1 cut(s) 845
BaeGI GKGCMC 1 cut(s) 818
BalI TGGCCA 1 cut(s) 414
BanI GGYRCC 2 cut(s) 815, 856
BanII GRGCYC 1 cut(s) 732
BauI CACGAG 1 cut(s) 1207
Bbv12I GWGCWC 2 cut(s) 732, 765
BccI CCATC 4 cut(s) 134, 404, 827, 992
BciT130I CCWGG 4 cut(s) 167, 344, 876, 892
BciVI GTATCC 1 cut(s) 882
BclI TGATCA 1 cut(s) 703
BcnI CCSGG 1 cut(s) 82
BcoDI GTCTC 2 cut(s) 140, 177
BfaI CTAG 5 cut(s) 204, 645, 846, 993, 1020
BfuI GTATCC 1 cut(s) 882
BisI GCNGC 1 cut(s) 1275
BlnI CCTAGG 1 cut(s) 845
BlsI GCNGC 1 cut(s) 1276
Bme1390I CCNGG 5 cut(s) 82, 167, 344, 876, 892
Bme18I GGWCC 1 cut(s) 879
BmeRI GACNNNNNGTC 1 cut(s) 1122
BmgT120I GGNCC 2 cut(s) 79, 879
BmiI GGNNCC 5 cut(s) 264, 430, 817, 858, 880
BmrFI CCNGG 5 cut(s) 82, 167, 344, 876, 892
BmrI ACTGGG 1 cut(s) 101
BmsI GCATC 5 cut(s) 52, 313, 366, 490, 1061
BmuI ACTGGG 1 cut(s) 101
BpmI CTGGAG 2 cut(s) 171, 977
Bpu10I CCTNAGC 1 cut(s) 476
BpuMI CCSGG 1 cut(s) 82
BsaBI GATNNNNATC 1 cut(s) 837
BsaJI CCNNGG 4 cut(s) 415, 845, 875, 1029
BsaXI ACNNNNNCTCC 2 cut(s) 886, 916
Bsc4I CCNNNNNNNGG 7 cut(s) 242, 309, 636, 717, 718, 1030, 1081
Bse1I ACTGG 3 cut(s) 107, 154, 1255
Bse3DI GCAATG 1 cut(s) 624
Bse8I GATNNNNATC 1 cut(s) 837
BseBI CCWGG 4 cut(s) 167, 344, 876, 892
BseDI CCNNGG 4 cut(s) 415, 845, 875, 1029
BseGI GGATG 8 cut(s) 357, 505, 571, 730, 973, 1076, 1110, 1167
BseJI GATNNNNATC 1 cut(s) 837
BseLI CCNNNNNNNGG 7 cut(s) 242, 309, 636, 717, 718, 1030, 1081
BseMI GCAATG 1 cut(s) 624
BseMII CTCAG 2 cut(s) 90, 906
BseNI ACTGG 3 cut(s) 107, 154, 1255
BseRI GAGGAG 1 cut(s) 689
BseSI GKGCMC 1 cut(s) 818
BsgI GTGCAG 1 cut(s) 824
Bsh1236I CGCG 2 cut(s) 519, 1144
BshFI GGCC 3 cut(s) 80, 338, 414
BshNI GGYRCC 2 cut(s) 815, 856
BsiHKAI GWGCWC 2 cut(s) 732, 765
BsiSI CCGG 3 cut(s) 82, 270, 1075
BslFI GGGAC 4 cut(s) 286, 388, 595, 892
BslI CCNNNNNNNGG 7 cut(s) 242, 309, 636, 717, 718, 1030, 1081
BsmAI GTCTC 2 cut(s) 140, 177
BsmBI CGTCTC 1 cut(s) 177
BsmFI GGGAC 4 cut(s) 286, 388, 595, 892
BsmI GAATGC 1 cut(s) 91
BsnI GGCC 3 cut(s) 80, 338, 414
Bsp1286I GDGCHC 3 cut(s) 732, 765, 818
Bsp1407I TGTACA 1 cut(s) 1258
Bsp19I CCATGG 1 cut(s) 415
Bsp68I TCGCGA 1 cut(s) 519
BspACI CCGC 8 cut(s) 659, 686, 842, 882, 1121, 1144, 1168, 1274
BspANI GGCC 3 cut(s) 80, 338, 414
BspCNI CTCAG 2 cut(s) 91, 907
BspFNI CGCG 2 cut(s) 519, 1144
BspLI GGNNCC 5 cut(s) 264, 430, 817, 858, 880
BspPI GGATC 5 cut(s) 97, 206, 699, 780, 833
BspT107I GGYRCC 2 cut(s) 815, 856
BsrDI GCAATG 1 cut(s) 624
BsrGI TGTACA 1 cut(s) 1258
BsrI ACTGG 3 cut(s) 107, 154, 1255
BssECI CCNNGG 4 cut(s) 415, 845, 875, 1029
BssSI CACGAG 1 cut(s) 1207
BssT1I CCWWGG 2 cut(s) 415, 845
Bst2BI CACGAG 1 cut(s) 1207
Bst2UI CCWGG 4 cut(s) 167, 344, 876, 892
Bst4CI ACNGT 4 cut(s) 228, 256, 598, 1030
Bst6I CTCTTC 1 cut(s) 130
BstAUI TGTACA 1 cut(s) 1258
BstDEI CTNAG 3 cut(s) 99, 476, 915
BstDSI CCRYGG 2 cut(s) 415, 1029
BstF5I GGATG 8 cut(s) 357, 505, 571, 730, 973, 1076, 1110, 1167
BstFNI CGCG 2 cut(s) 519, 1144
BstHHI GCGC 2 cut(s) 1052, 1224
BstMAI GTCTC 2 cut(s) 140, 177
BstMWI GCNNNNNNNGC 6 cut(s) 86, 509, 530, 656, 856, 1071
BstNI CCWGG 4 cut(s) 167, 344, 876, 892
BstSCI CCNGG 5 cut(s) 80, 165, 342, 874, 890
BstSLI GKGCMC 1 cut(s) 818
BstUI CGCG 2 cut(s) 519, 1144
BstX2I RGATCY 2 cut(s) 102, 691
BstYI RGATCY 2 cut(s) 102, 691
BsuI GTATCC 1 cut(s) 882
BsuRI GGCC 3 cut(s) 80, 338, 414
BtgI CCRYGG 2 cut(s) 415, 1029
BtsCI GGATG 8 cut(s) 357, 505, 571, 730, 973, 1076, 1110, 1167
BtsI GCAGTG 2 cut(s) 812, 1059
BtsIMutI CAGTG 4 cut(s) 96, 812, 1059, 1248
BtuMI TCGCGA 1 cut(s) 519
CfoI GCGC 2 cut(s) 1052, 1224
Cfr13I GGNCC 2 cut(s) 79, 879
CseI GACGC 1 cut(s) 194
Csp6I GTAC 2 cut(s) 289, 1259
CviAII CATG 5 cut(s) 416, 482, 551, 574, 1097
CviQI GTAC 2 cut(s) 289, 1259
DdeI CTNAG 3 cut(s) 99, 476, 915
DriI GACNNNNNGTC 1 cut(s) 1122
EaeI YGGCCR 1 cut(s) 412
Eam1104I CTCTTC 1 cut(s) 130
Eam1105I GACNNNNNGTC 1 cut(s) 1122
EarI CTCTTC 1 cut(s) 130
EciI GGCGGA 1 cut(s) 831
Ecl136II GAGCTC 1 cut(s) 730
Eco130I CCWWGG 2 cut(s) 415, 845
Eco24I GRGCYC 1 cut(s) 732
Eco47I GGWCC 1 cut(s) 879
Eco53kI GAGCTC 1 cut(s) 730
Eco57I CTGAAG 1 cut(s) 809
EcoICRI GAGCTC 1 cut(s) 730
EcoRI GAATTC 1 cut(s) 538
EcoRII CCWGG 4 cut(s) 165, 342, 874, 890
EcoT14I CCWWGG 2 cut(s) 415, 845
EcoT38I GRGCYC 1 cut(s) 732
ErhI CCWWGG 2 cut(s) 415, 845
Esp3I CGTCTC 1 cut(s) 177
FaeI CATG 5 cut(s) 419, 485, 554, 577, 1100
FaqI GGGAC 4 cut(s) 286, 388, 595, 892
FatI CATG 5 cut(s) 415, 481, 550, 573, 1096
FauI CCCGC 2 cut(s) 1114, 1161
FbaI TGATCA 1 cut(s) 703
Fnu4HI GCNGC 1 cut(s) 1275
FokI GGATG 8 cut(s) 344, 512, 578, 737, 960, 1083, 1117, 1174
FriOI GRGCYC 1 cut(s) 732
Fsp4HI GCNGC 1 cut(s) 1275
FspBI CTAG 5 cut(s) 204, 645, 846, 993, 1020
FspI TGCGCA 1 cut(s) 1051
GlaI GCGC 2 cut(s) 1051, 1223
GluI GCNGC 1 cut(s) 1275
GsuI CTGGAG 2 cut(s) 171, 977
HaeIII GGCC 3 cut(s) 80, 338, 414
HapII CCGG 3 cut(s) 82, 270, 1075
HgaI GACGC 1 cut(s) 194
HhaI GCGC 2 cut(s) 1052, 1224
Hin1II CATG 5 cut(s) 419, 485, 554, 577, 1100
Hin6I GCGC 2 cut(s) 1050, 1222
HinP1I GCGC 2 cut(s) 1050, 1222
HincII GTYRAC 1 cut(s) 1189
HindII GTYRAC 1 cut(s) 1189
HinfI GANTC 6 cut(s) 641, 793, 905, 917, 970, 1139
HpaII CCGG 3 cut(s) 82, 270, 1075
HphI GGTGA 5 cut(s) 108, 155, 839, 848, 914
Hpy166II GTNNAC 2 cut(s) 1189, 1259
Hpy188I TCNGA 3 cut(s) 708, 777, 1242
Hpy188III TCNNGA 8 cut(s) 57, 249, 329, 451, 518, 645, 797, 956
Hpy8I GTNNAC 2 cut(s) 1189, 1259
Hpy99I CGWCG 1 cut(s) 1235
HpyAV CCTTC 3 cut(s) 63, 776, 818
HpyCH4III ACNGT 4 cut(s) 228, 256, 598, 1030
HpyCH4IV ACGT 2 cut(s) 1191, 1233
HpyCH4V TGCA 4 cut(s) 357, 550, 755, 805
HpyF10VI GCNNNNNNNGC 6 cut(s) 86, 509, 530, 656, 856, 1071
HpyF3I CTNAG 3 cut(s) 99, 476, 915
HpySE526I ACGT 2 cut(s) 1191, 1233
Hsp92II CATG 5 cut(s) 419, 485, 554, 577, 1100
HspAI GCGC 2 cut(s) 1050, 1222
Ksp22I TGATCA 1 cut(s) 703
LmnI GCTCC 1 cut(s) 958
LweI GCATC 5 cut(s) 52, 313, 366, 490, 1061
MaeI CTAG 5 cut(s) 204, 645, 846, 993, 1020
MaeII ACGT 2 cut(s) 1191, 1233
MaeIII GTNAC 2 cut(s) 1192, 1244
MboII GAAGA 5 cut(s) 117, 628, 802, 833, 973
MfeI CAATTG 1 cut(s) 921
MflI RGATCY 2 cut(s) 102, 691
MhlI GDGCHC 3 cut(s) 732, 765, 818
MlsI TGGCCA 1 cut(s) 414
MluCI AATT 5 cut(s) 469, 538, 631, 921, 945
MluNI TGGCCA 1 cut(s) 414
MlyI GAGTC 1 cut(s) 926
Mox20I TGGCCA 1 cut(s) 414
MscI TGGCCA 1 cut(s) 414
MseI TTAA 1 cut(s) 741
Msp20I TGGCCA 1 cut(s) 414
MspI CCGG 3 cut(s) 82, 270, 1075
MspR9I CCNGG 5 cut(s) 82, 167, 344, 876, 892
MunI CAATTG 1 cut(s) 921
Mva1269I GAATGC 1 cut(s) 91
MvaI CCWGG 4 cut(s) 167, 344, 876, 892
MvnI CGCG 2 cut(s) 519, 1144
MwoI GCNNNNNNNGC 6 cut(s) 86, 509, 530, 656, 856, 1071
NciI CCSGG 1 cut(s) 82
NcoI CCATGG 1 cut(s) 415
NlaIII CATG 5 cut(s) 419, 485, 554, 577, 1100
NlaIV GGNNCC 5 cut(s) 264, 430, 817, 858, 880
NruI TCGCGA 1 cut(s) 519
NsbI TGCGCA 1 cut(s) 1051
PcsI WCGNNNNNNNCGW 1 cut(s) 1261
PctI GAATGC 1 cut(s) 91
PfeI GAWTC 5 cut(s) 641, 793, 905, 970, 1139
PflMI CCANNNNNTGG 1 cut(s) 1030
PkrI GCNGC 1 cut(s) 1276
PleI GAGTC 1 cut(s) 925
PpsI GAGTC 1 cut(s) 925
Psp124BI GAGCTC 1 cut(s) 732
Psp6I CCWGG 4 cut(s) 165, 342, 874, 890
PspGI CCWGG 4 cut(s) 165, 342, 874, 890
PspN4I GGNNCC 5 cut(s) 264, 430, 817, 858, 880
PspPI GGNCC 2 cut(s) 79, 879
PsrI GAACNNNNNNTAC 2 cut(s) 37, 69
PsuI RGATCY 2 cut(s) 102, 691
RruI TCGCGA 1 cut(s) 519
RsaI GTAC 2 cut(s) 290, 1260
RsaNI GTAC 2 cut(s) 289, 1259
SacI GAGCTC 1 cut(s) 732
SaqAI TTAA 1 cut(s) 741
SatI GCNGC 1 cut(s) 1275
Sau96I GGNCC 2 cut(s) 79, 879
SchI GAGTC 1 cut(s) 926
ScrFI CCNGG 5 cut(s) 82, 167, 344, 876, 892
SduI GDGCHC 3 cut(s) 732, 765, 818
SfaNI GCATC 5 cut(s) 52, 313, 366, 490, 1061
SinI GGWCC 1 cut(s) 879
Sse9I AATT 5 cut(s) 469, 538, 631, 921, 945
SsiI CCGC 8 cut(s) 659, 686, 842, 882, 1121, 1144, 1168, 1274
SspMI CTAG 5 cut(s) 204, 645, 846, 993, 1020
SstI GAGCTC 1 cut(s) 732
StyD4I CCNGG 5 cut(s) 80, 165, 342, 874, 890
StyI CCWWGG 2 cut(s) 415, 845
TaaI ACNGT 4 cut(s) 228, 256, 598, 1030
TaiI ACGT 2 cut(s) 1194, 1236
TaqI TCGA 9 cut(s) 17, 212, 248, 330, 507, 698, 732, 908, 1126
TasI AATT 5 cut(s) 469, 538, 631, 921, 945
TatI WGTACW 1 cut(s) 1258
TauI GCSGC 1 cut(s) 1277
TfiI GAWTC 5 cut(s) 641, 793, 905, 970, 1139
Tru1I TTAA 1 cut(s) 741
Tru9I TTAA 1 cut(s) 741
TscAI CASTG 4 cut(s) 103, 812, 1059, 1255
TspGWI ACGGA 2 cut(s) 357, 1188
TspRI CASTG 4 cut(s) 103, 812, 1059, 1255
Van91I CCANNNNNTGG 1 cut(s) 1030
VpaK11BI GGWCC 1 cut(s) 879
XapI RAATTY 2 cut(s) 469, 538
XbaI TCTAGA 1 cut(s) 644
XcmI CCANNNNNNNNNTGG 3 cut(s) 368, 422, 598
XmaJI CCTAGG 1 cut(s) 845
XspI CTAG 5 cut(s) 204, 645, 846, 993, 1020
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.