Rmu_ssc0000412.1_g000013

psbP domain-containing protein 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000412.1
OG0008432
Physical Location & Seq
Reverse (-)
99490 .. 101429
1940 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 759 bp
atggttattctctctccctctctttctctcttcacatccaaatccaatcactttcttgttcctccaaacctcttcttcttcagagagcacaacagagcagtggcgcaccagcagagcaaatgctcaaaacccaatttccattatgggttttgcagaagagacttattgctgtttggcctttcttctccactcttaacagttttgccatcttcagggtcccttgcagaagaagatgtgaaaatggcttcgtttgctgatgacataaattcttattcttatctctacccgctggagttgcaatctaagaaggtcatcttcaaatgggtggaatcgagaaagccggaacgctattcatcagctgctccattatctcctgatgcacgccttcgcattgtctctgagcgtgttgacattatcgataatctcataatttctatttcgataggtcccccaaattcaaagattttaaaatcaccggacaagagtacctggactgcaaaagatgttgctgattctgttttggctgacaagtctgcattgcgggttacctcaactcagcgaatggctgagagttcagttcttgatgcacatactagtcaaattgatggtcagccatattggaattttgagtaccttgtccgcaaagcacccacaaaaactgctcaagaatctaacagttaccggcactatgtggcttcaacaaccgaacgagatggtggggcaaatgcttcttctgtgtacatctattctaccgcttga

Protein Analysis

252

Amino Acids

28.16

Weight (kDa)

8.94

Isoelectric Point (pI)

44.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsbP PF01789 81 - 238 7.3e-06 PsbP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 287, 541, 640, 753
AcsI RAATTY 3 cut(s) 265, 454, 622
AcuI CTGAAG 2 cut(s) 64, 195
AdeI CACNNNGTG 1 cut(s) 691
AfaI GTAC 3 cut(s) 487, 632, 740
AfiI CCNNNNNNNGG 1 cut(s) 212
AgsI TTSAA 3 cut(s) 319, 459, 699
AhlI ACTAGT 1 cut(s) 593
AjnI CCWGG 1 cut(s) 488
AluBI AGCT 1 cut(s) 359
AluI AGCT 1 cut(s) 359
Alw21I GWGCWC 1 cut(s) 90
Alw26I GTCTC 2 cut(s) 153, 400
AoxI GGCC 1 cut(s) 175
ApeKI GCWGC 1 cut(s) 359
ApoI RAATTY 3 cut(s) 265, 454, 622
ArsI GACNNNNNNTTYG 2 cut(s) 592, 624
AspLEI GCGC 1 cut(s) 106
AspS9I GGNCC 2 cut(s) 216, 446
AsuHPI GGTGA 1 cut(s) 465
AvaII GGWCC 2 cut(s) 216, 446
BaeI ACNNNNGTAYC 2 cut(s) 469, 502
Bbv12I GWGCWC 1 cut(s) 90
BbvI GCAGC 1 cut(s) 346
BccI CCATC 3 cut(s) 214, 599, 707
BciT130I CCWGG 1 cut(s) 490
BcoDI GTCTC 2 cut(s) 153, 400
BcuI ACTAGT 1 cut(s) 593
BfaI CTAG 1 cut(s) 594
BisI GCNGC 1 cut(s) 360
BlsI GCNGC 1 cut(s) 361
Bme1390I CCNGG 1 cut(s) 490
Bme18I GGWCC 2 cut(s) 216, 446
BmgT120I GGNCC 2 cut(s) 216, 446
BmiI GGNNCC 3 cut(s) 217, 218, 448
BmrFI CCNGG 1 cut(s) 490
BmsI GCATC 2 cut(s) 367, 574
BpmI CTGGAG 1 cut(s) 311
BpuEI CTTGAG 1 cut(s) 648
Bsa29I ATCGAT 1 cut(s) 417
BsaWI WCCGGW 1 cut(s) 475
Bsc4I CCNNNNNNNGG 1 cut(s) 212
Bse118I RCCGGY 1 cut(s) 681
Bse3DI GCAATG 1 cut(s) 536
BseBI CCWGG 1 cut(s) 490
BseCI ATCGAT 1 cut(s) 417
BseGI GGATG 1 cut(s) 35
BseLI CCNNNNNNNGG 1 cut(s) 212
BseMI GCAATG 1 cut(s) 536
BseMII CTCAG 3 cut(s) 390, 558, 569
BseXI GCAGC 1 cut(s) 346
BshFI GGCC 1 cut(s) 177
BshVI ATCGAT 1 cut(s) 417
BsiHKAI GWGCWC 1 cut(s) 90
BsiSI CCGG 3 cut(s) 341, 476, 682
BslFI GGGAC 2 cut(s) 202, 432
BslI CCNNNNNNNGG 1 cut(s) 212
BsmAI GTCTC 2 cut(s) 153, 400
BsmFI GGGAC 2 cut(s) 202, 432
BsnI GGCC 1 cut(s) 177
Bsp1286I GDGCHC 1 cut(s) 90
Bsp1407I TGTACA 1 cut(s) 738
BspACI CCGC 4 cut(s) 287, 541, 640, 753
BspANI GGCC 1 cut(s) 177
BspCNI CTCAG 3 cut(s) 391, 559, 568
BspDI ATCGAT 1 cut(s) 417
BspLI GGNNCC 3 cut(s) 217, 218, 448
BsrDI GCAATG 1 cut(s) 536
BsrFI RCCGGY 1 cut(s) 681
BsrGI TGTACA 1 cut(s) 738
BssAI RCCGGY 1 cut(s) 681
Bst2UI CCWGG 1 cut(s) 490
Bst4CI ACNGT 2 cut(s) 199, 677
Bst6I CTCTTC 3 cut(s) 35, 77, 151
BstAUI TGTACA 1 cut(s) 738
BstC8I GCNNGC 1 cut(s) 382
BstDEI CTNAG 4 cut(s) 303, 399, 555, 567
BstEII GGTNACC 1 cut(s) 544
BstF5I GGATG 1 cut(s) 35
BstHHI GCGC 1 cut(s) 106
BstMAI GTCTC 2 cut(s) 153, 400
BstMWI GCNNNNNNNGC 2 cut(s) 251, 295
BstNI CCWGG 1 cut(s) 490
BstPI GGTNACC 1 cut(s) 544
BstSCI CCNGG 1 cut(s) 488
BstV1I GCAGC 1 cut(s) 346
Bsu15I ATCGAT 1 cut(s) 417
BsuRI GGCC 1 cut(s) 177
BsuTUI ATCGAT 1 cut(s) 417
BtsCI GGATG 1 cut(s) 35
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 1 cut(s) 105
Cac8I GCNNGC 1 cut(s) 382
CfoI GCGC 1 cut(s) 106
Cfr10I RCCGGY 1 cut(s) 681
Cfr13I GGNCC 2 cut(s) 216, 446
ClaI ATCGAT 1 cut(s) 417
Csp6I GTAC 3 cut(s) 486, 631, 739
CviJI RGCY 8 cut(s) 177, 245, 340, 359, 524, 566, 613, 695
CviKI_1 RGCY 8 cut(s) 177, 245, 340, 359, 524, 566, 613, 695
CviQI GTAC 3 cut(s) 486, 631, 739
DdeI CTNAG 4 cut(s) 303, 399, 555, 567
DraI TTTAAA 1 cut(s) 468
DraIII CACNNNGTG 1 cut(s) 691
Eam1104I CTCTTC 3 cut(s) 35, 77, 151
EarI CTCTTC 3 cut(s) 35, 77, 151
Eco47I GGWCC 2 cut(s) 216, 446
Eco57I CTGAAG 2 cut(s) 64, 195
Eco91I GGTNACC 1 cut(s) 544
EcoO109I RGGNCCY 2 cut(s) 216, 446
EcoO65I GGTNACC 1 cut(s) 544
EcoRII CCWGG 1 cut(s) 488
FaiI YATR 6 cut(s) 144, 263, 428, 591, 616, 690
FalI AAGNNNNNCTT 2 cut(s) 299, 331
FaqI GGGAC 2 cut(s) 202, 432
FauI CCCGC 2 cut(s) 294, 534
Fnu4HI GCNGC 1 cut(s) 360
FokI GGATG 1 cut(s) 22
Fsp4HI GCNGC 1 cut(s) 360
FspBI CTAG 1 cut(s) 594
GlaI GCGC 1 cut(s) 105
GluI GCNGC 1 cut(s) 360
GsuI CTGGAG 1 cut(s) 311
HaeIII GGCC 1 cut(s) 177
HapII CCGG 3 cut(s) 341, 476, 682
HhaI GCGC 1 cut(s) 106
Hin6I GCGC 1 cut(s) 104
HinP1I GCGC 1 cut(s) 104
HincII GTYRAC 1 cut(s) 409
HindII GTYRAC 1 cut(s) 409
HinfI GANTC 3 cut(s) 329, 512, 668
HpaII CCGG 3 cut(s) 341, 476, 682
HphI GGTGA 1 cut(s) 465
Hpy166II GTNNAC 2 cut(s) 409, 739
Hpy188I TCNGA 2 cut(s) 83, 400
Hpy188III TCNNGA 4 cut(s) 333, 374, 581, 665
Hpy8I GTNNAC 2 cut(s) 409, 739
HpyAV CCTTC 2 cut(s) 301, 395
HpyCH4III ACNGT 2 cut(s) 199, 677
HpyCH4V TGCA 7 cut(s) 153, 224, 298, 380, 497, 536, 587
HpyF10VI GCNNNNNNNGC 2 cut(s) 251, 295
HpyF3I CTNAG 4 cut(s) 303, 399, 555, 567
HspAI GCGC 1 cut(s) 104
KflI GGGWCCC 1 cut(s) 216
LmnI GCTCC 1 cut(s) 367
LpnPI CCDG 9 cut(s) 122, 198, 275, 354, 387, 475, 489, 502, 695
Lsp1109I GCAGC 1 cut(s) 346
LweI GCATC 2 cut(s) 367, 574
MaeI CTAG 1 cut(s) 594
MaeIII GTNAC 2 cut(s) 544, 677
MhlI GDGCHC 1 cut(s) 90
MluCI AATT 6 cut(s) 133, 265, 429, 454, 600, 622
MnlI CCTC 4 cut(s) 28, 72, 80, 559
MseI TTAA 2 cut(s) 194, 467
MspA1I CMGCKG 2 cut(s) 289, 359
MspI CCGG 3 cut(s) 341, 476, 682
MspR9I CCNGG 1 cut(s) 490
MvaI CCWGG 1 cut(s) 490
MwoI GCNNNNNNNGC 2 cut(s) 251, 295
NlaIV GGNNCC 3 cut(s) 217, 218, 448
PfeI GAWTC 3 cut(s) 329, 512, 668
PkrI GCNGC 1 cut(s) 361
PpuMI RGGWCCY 2 cut(s) 216, 446
Psp5II RGGWCCY 2 cut(s) 216, 446
Psp6I CCWGG 1 cut(s) 488
PspEI GGTNACC 1 cut(s) 544
PspGI CCWGG 1 cut(s) 488
PspN4I GGNNCC 3 cut(s) 217, 218, 448
PspPI GGNCC 2 cut(s) 216, 446
PspPPI RGGWCCY 2 cut(s) 216, 446
PvuII CAGCTG 1 cut(s) 359
RsaI GTAC 3 cut(s) 487, 632, 740
RsaNI GTAC 3 cut(s) 486, 631, 739
SaqAI TTAA 2 cut(s) 194, 467
SatI GCNGC 1 cut(s) 360
Sau96I GGNCC 2 cut(s) 216, 446
ScrFI CCNGG 1 cut(s) 490
SduI GDGCHC 1 cut(s) 90
SetI ASST 7 cut(s) 72, 312, 361, 448, 491, 551, 636
SfaNI GCATC 2 cut(s) 367, 574
SinI GGWCC 2 cut(s) 216, 446
SmlI CTYRAG 1 cut(s) 663
SmoI CTYRAG 1 cut(s) 663
SpeI ACTAGT 1 cut(s) 593
Sse9I AATT 6 cut(s) 133, 265, 429, 454, 600, 622
SsiI CCGC 4 cut(s) 287, 541, 640, 753
SspMI CTAG 1 cut(s) 594
StyD4I CCNGG 1 cut(s) 488
TaaI ACNGT 2 cut(s) 199, 677
TaqI TCGA 3 cut(s) 332, 417, 440
TasI AATT 6 cut(s) 133, 265, 429, 454, 600, 622
TatI WGTACW 1 cut(s) 738
TfiI GAWTC 3 cut(s) 329, 512, 668
Tru1I TTAA 2 cut(s) 194, 467
Tru9I TTAA 2 cut(s) 194, 467
TscAI CASTG 1 cut(s) 105
TseI GCWGC 1 cut(s) 359
TspDTI ATGAA 1 cut(s) 342
TspRI CASTG 1 cut(s) 105
VpaK11BI GGWCC 2 cut(s) 216, 446
XapI RAATTY 3 cut(s) 265, 454, 622
XspI CTAG 1 cut(s) 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.