Rmu_ssc0000445.1_g000001

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000445.1
OG0006535
Physical Location & Seq
Forward (+)
79 .. 1141
1063 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 582 bp
atgataggggaagccgaagccaatgccatattctctggcgccatccaccttcttagcgcaggaaccagcgatttcattcagaactactacatcaatccatttcttagaggctacacacctgaccaattctcagaaattctaatgagatcctactcctctttcattcagaagttgtatggactcggagcacgaaggattggagtcacaaacttgccacctataggatgtttgccagcagcaattaccctatttggctcaggaagcaaccagtggaacataagattgaatcaagatgccattttattcaataacaagcttaacaacacatctcaaactttgcagaagagtcttcccggactcaagctcgtagtttttgatatctaccagcccctcctggatatgatcacaaacccccgtgacaatggtacatttgaaacatctgtgctctgcaactctaggtctgtaggaacatgcaacaatgcaactggttatgtgttctgggatggatttcatccctctgaagctgccaatgaggtcttagctggtaatctacttgcacagggcatcgatctcatatcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

193

Amino Acids

21.09

Weight (kDa)

5.24

Isoelectric Point (pI)

30.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 10 - 181 1.3e-19 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 38
AclWI GGATC 1 cut(s) 141
AcsI RAATTY 1 cut(s) 135
AcuI CTGAAG 1 cut(s) 540
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 1 cut(s) 427
AfiI CCNNNNNNNGG 1 cut(s) 221
AgsI TTSAA 3 cut(s) 286, 307, 434
AjnI CCWGG 1 cut(s) 393
AjuI GAANNNNNNNTTGG 2 cut(s) 14, 46
AluBI AGCT 4 cut(s) 316, 364, 524, 542
AluI AGCT 4 cut(s) 316, 364, 524, 542
Alw21I GWGCWC 2 cut(s) 190, 447
AlwI GGATC 1 cut(s) 141
ApeKI GCWGC 2 cut(s) 236, 524
ApoI RAATTY 1 cut(s) 135
AspLEI GCGC 2 cut(s) 41, 59
AsuC2I CCSGG 1 cut(s) 354
BanI GGYRCC 1 cut(s) 38
BbsI GAAGAC 1 cut(s) 341
Bbv12I GWGCWC 2 cut(s) 190, 447
BbvI GCAGC 2 cut(s) 248, 511
BccI CCATC 2 cut(s) 50, 497
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BciT130I CCWGG 1 cut(s) 395
BclI TGATCA 1 cut(s) 402
BcnI CCSGG 1 cut(s) 354
BfaI CTAG 1 cut(s) 456
BfmI CTRYAG 2 cut(s) 219, 462
BfoI RGCGCY 1 cut(s) 42
BisI GCNGC 2 cut(s) 237, 525
BlsI GCNGC 2 cut(s) 238, 526
Bme1390I CCNGG 2 cut(s) 354, 395
BmiI GGNNCC 2 cut(s) 40, 64
BmrFI CCNGG 2 cut(s) 354, 395
BmsI GCATC 2 cut(s) 283, 573
BpiI GAAGAC 1 cut(s) 341
Bpu10I CCTNAGC 1 cut(s) 256
BpuEI CTTGAG 1 cut(s) 344
BpuMI CCSGG 1 cut(s) 354
Bsa29I ATCGAT 1 cut(s) 567
BsaHI GRCGYC 1 cut(s) 39
Bsc4I CCNNNNNNNGG 1 cut(s) 221
Bse1I ACTGG 2 cut(s) 268, 490
BseBI CCWGG 1 cut(s) 395
BseCI ATCGAT 1 cut(s) 567
BseGI GGATG 4 cut(s) 42, 230, 508, 511
BseLI CCNNNNNNNGG 1 cut(s) 221
BseMII CTCAG 2 cut(s) 144, 270
BseNI ACTGG 2 cut(s) 268, 490
BseRI GAGGAG 1 cut(s) 145
BseXI GCAGC 2 cut(s) 248, 511
BshNI GGYRCC 1 cut(s) 38
BshVI ATCGAT 1 cut(s) 567
BsiHKAI GWGCWC 2 cut(s) 190, 447
BsiSI CCGG 1 cut(s) 354
BslI CCNNNNNNNGG 1 cut(s) 221
Bsp1286I GDGCHC 2 cut(s) 190, 447
Bsp143I GATC 3 cut(s) 146, 402, 568
BspCNI CTCAG 2 cut(s) 143, 269
BspDI ATCGAT 1 cut(s) 567
BspLI GGNNCC 2 cut(s) 40, 64
BspPI GGATC 1 cut(s) 141
BspT107I GGYRCC 1 cut(s) 38
BsrI ACTGG 2 cut(s) 268, 490
BssMI GATC 3 cut(s) 146, 402, 568
BssNI GRCGYC 1 cut(s) 39
Bst2UI CCWGG 1 cut(s) 395
Bst6I CTCTTC 1 cut(s) 338
BstACI GRCGYC 1 cut(s) 39
BstC8I GCNNGC 1 cut(s) 234
BstDEI CTNAG 5 cut(s) 53, 104, 130, 256, 538
BstF5I GGATG 4 cut(s) 42, 230, 508, 511
BstH2I RGCGCY 1 cut(s) 42
BstHHI GCGC 2 cut(s) 41, 59
BstKTI GATC 3 cut(s) 149, 405, 571
BstMBI GATC 3 cut(s) 146, 402, 568
BstMWI GCNNNNNNNGC 1 cut(s) 261
BstNI CCWGG 1 cut(s) 395
BstNSI RCATGY 1 cut(s) 474
BstSCI CCNGG 2 cut(s) 352, 393
BstSFI CTRYAG 2 cut(s) 219, 462
BstV1I GCAGC 2 cut(s) 248, 511
BstV2I GAAGAC 1 cut(s) 341
BstX2I RGATCY 1 cut(s) 146
BstYI RGATCY 1 cut(s) 146
Bsu15I ATCGAT 1 cut(s) 567
BsuTUI ATCGAT 1 cut(s) 567
BtsCI GGATG 4 cut(s) 42, 230, 508, 511
BtsIMutI CAGTG 1 cut(s) 275
Cac8I GCNNGC 1 cut(s) 234
CfoI GCGC 2 cut(s) 41, 59
ClaI ATCGAT 1 cut(s) 567
Csp6I GTAC 1 cut(s) 426
CviAII CATG 1 cut(s) 471
CviJI RGCY 9 cut(s) 14, 20, 111, 255, 316, 364, 388, 524, 542
CviKI_1 RGCY 9 cut(s) 14, 20, 111, 255, 316, 364, 388, 524, 542
CviQI GTAC 1 cut(s) 426
DdeI CTNAG 5 cut(s) 53, 104, 130, 256, 538
DinI GGCGCC 1 cut(s) 40
DpnI GATC 3 cut(s) 148, 404, 570
DpnII GATC 3 cut(s) 146, 402, 568
Eam1104I CTCTTC 1 cut(s) 338
EarI CTCTTC 1 cut(s) 338
Eco32I GATATC 1 cut(s) 379
Eco57I CTGAAG 1 cut(s) 540
EcoRII CCWGG 1 cut(s) 393
EcoRV GATATC 1 cut(s) 379
EgeI GGCGCC 1 cut(s) 40
EheI GGCGCC 1 cut(s) 40
FaeI CATG 1 cut(s) 474
FaiI YATR 8 cut(s) 29, 177, 221, 278, 401, 472, 492, 575
FatI CATG 1 cut(s) 470
FbaI TGATCA 1 cut(s) 402
Fnu4HI GCNGC 2 cut(s) 237, 525
FokI GGATG 4 cut(s) 29, 237, 498, 515
Fsp4HI GCNGC 2 cut(s) 237, 525
FspBI CTAG 1 cut(s) 456
GlaI GCGC 2 cut(s) 40, 58
GluI GCNGC 2 cut(s) 237, 525
HaeII RGCGCY 1 cut(s) 42
HapII CCGG 1 cut(s) 354
HhaI GCGC 2 cut(s) 41, 59
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 1 cut(s) 474
Hin6I GCGC 2 cut(s) 39, 57
HinP1I GCGC 2 cut(s) 39, 57
HindIII AAGCTT 1 cut(s) 314
HinfI GANTC 5 cut(s) 180, 201, 286, 346, 357
HpaII CCGG 1 cut(s) 354
Hpy188I TCNGA 5 cut(s) 81, 133, 168, 185, 520
Hpy188III TCNNGA 3 cut(s) 258, 290, 579
HpyAV CCTTC 2 cut(s) 59, 186
HpyCH4V TGCA 5 cut(s) 340, 450, 474, 482, 557
HpyF10VI GCNNNNNNNGC 1 cut(s) 261
HpyF3I CTNAG 5 cut(s) 53, 104, 130, 256, 538
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 1 cut(s) 474
HspAI GCGC 2 cut(s) 39, 57
KasI GGCGCC 1 cut(s) 38
Ksp22I TGATCA 1 cut(s) 402
Kzo9I GATC 3 cut(s) 146, 402, 568
LmnI GCTCC 1 cut(s) 185
Lsp1109I GCAGC 2 cut(s) 248, 511
LweI GCATC 2 cut(s) 283, 573
MaeI CTAG 1 cut(s) 456
MaeIII GTNAC 2 cut(s) 202, 416
MalI GATC 3 cut(s) 148, 404, 570
MboI GATC 3 cut(s) 146, 402, 568
MboII GAAGA 2 cut(s) 341, 355
MflI RGATCY 1 cut(s) 146
MhlI GDGCHC 2 cut(s) 190, 447
MluCI AATT 3 cut(s) 125, 135, 240
Mly113I GGCGCC 1 cut(s) 39
MlyI GAGTC 4 cut(s) 174, 210, 351, 355
MnlI CCTC 5 cut(s) 101, 166, 401, 526, 526
MseI TTAA 1 cut(s) 318
MspI CCGG 1 cut(s) 354
MspR9I CCNGG 2 cut(s) 354, 395
MvaI CCWGG 1 cut(s) 395
MwoI GCNNNNNNNGC 1 cut(s) 261
NarI GGCGCC 1 cut(s) 39
NciI CCSGG 1 cut(s) 354
NdeII GATC 3 cut(s) 146, 402, 568
NlaIII CATG 1 cut(s) 474
NlaIV GGNNCC 2 cut(s) 40, 64
NmuCI GTSAC 2 cut(s) 202, 416
NspI RCATGY 1 cut(s) 474
PfeI GAWTC 1 cut(s) 286
PfoI TCCNGGA 2 cut(s) 352, 393
PkrI GCNGC 2 cut(s) 238, 526
PleI GAGTC 4 cut(s) 174, 209, 351, 354
PluTI GGCGCC 1 cut(s) 42
PpsI GAGTC 4 cut(s) 174, 209, 351, 354
Psp6I CCWGG 1 cut(s) 393
PspGI CCWGG 1 cut(s) 393
PspN4I GGNNCC 2 cut(s) 40, 64
PsuI RGATCY 1 cut(s) 146
RsaI GTAC 1 cut(s) 427
RsaNI GTAC 1 cut(s) 426
SaqAI TTAA 1 cut(s) 318
SatI GCNGC 2 cut(s) 237, 525
Sau3AI GATC 3 cut(s) 146, 402, 568
SchI GAGTC 4 cut(s) 174, 210, 351, 355
ScrFI CCNGG 2 cut(s) 354, 395
SduI GDGCHC 2 cut(s) 190, 447
SetI ASST 9 cut(s) 51, 121, 220, 318, 366, 461, 526, 537, 544
SfaNI GCATC 2 cut(s) 283, 573
SfcI CTRYAG 2 cut(s) 219, 462
SfoI GGCGCC 1 cut(s) 40
SmlI CTYRAG 1 cut(s) 359
SmoI CTYRAG 1 cut(s) 359
Sse9I AATT 3 cut(s) 125, 135, 240
SspDI GGCGCC 1 cut(s) 38
SspMI CTAG 1 cut(s) 456
StyD4I CCNGG 2 cut(s) 352, 393
TaqI TCGA 1 cut(s) 567
TasI AATT 3 cut(s) 125, 135, 240
TfiI GAWTC 1 cut(s) 286
Tru1I TTAA 1 cut(s) 318
Tru9I TTAA 1 cut(s) 318
TscAI CASTG 1 cut(s) 275
TseFI GTSAC 2 cut(s) 202, 416
TseI GCWGC 2 cut(s) 236, 524
Tsp45I GTSAC 2 cut(s) 202, 416
TspDTI ATGAA 3 cut(s) 64, 151, 500
TspRI CASTG 1 cut(s) 275
XapI RAATTY 1 cut(s) 135
XceI RCATGY 1 cut(s) 474
XspI CTAG 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.