Rroxscaffold_165G00437030

UDP-N-acetylglucosamine transferase subunit ALG13 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000165
OG0004463
Physical Location & Seq
Reverse (-)
132593 .. 142375
9783 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_165G00437030.1

Sequence Viewer

Length: 657 bp
ATGGGGAGGAAGAGACAGACTCATGATGGCGAGGTGGCGCCGTTGAAGGCCATCAAGGACAGGCTGCCGCCAACTTTTGCAAACTGCAGTAAGACGAACAAGATGGTGAAACGAGACAAGATGGGAGATACTGAGGTAACAATGAAGTCTAAGAAGACGGTTTTTGTAACTGTTGGAACTACTAGTTTTGATGCTCTAGTGAAAGCGGTGGACACACGAGAGGTTAAAGAAGAGCTGTCAAGAAGAGGGTACACTGAGATTCTCATTCAAATGGGCCGTGGCTCCTATATTCCCTCAAAGTCTGACGGAGGTGATGACTCCATTGCGGTAGATTACTTCACTTTTTCATCAAGCATTGCAGACTATCTGAGAGCAGCATCTCTCGTCATTAGCCATGCAGGGTCAGGGAGCATATTTGAGACATTGCGGCTTGGGAAGCCTTTAATTGTTGTGGTTAATGAAGATTTGATGGACAACCATCAAAGTGAGTTGGCAGAAGAACTAGCAGACAGGAAGCATCTGTACTATGCTCACCCAAAGACACTTCAACACATTATAGCAGATATGAAAGTGGAGTCCCTCATTCCATACCATCCAGGTGATGCCACACCAGTCGCCAAGCTTATCAATAGGTTTCTTGGTTTTTCAGAGGATTGA

Protein Analysis

218

Amino Acids

24.21

Weight (kDa)

7.07

Isoelectric Point (pI)

37.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_tran_28_C PF04101 53 - 202 9.7e-35 Glycosyltransferase family 28 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 37
AciI CCGC 4 cut(s) 68, 206, 326, 427
AcyI GRCGYC 1 cut(s) 38
AfaI GTAC 2 cut(s) 251, 524
AgsI TTSAA 3 cut(s) 46, 269, 548
AhlI ACTAGT 1 cut(s) 182
AjnI CCWGG 1 cut(s) 595
AluBI AGCT 2 cut(s) 235, 622
AluI AGCT 2 cut(s) 235, 622
Alw26I GTCTC 3 cut(s) 7, 108, 413
AoxI GGCC 2 cut(s) 48, 274
ApeKI GCWGC 2 cut(s) 64, 374
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 1 cut(s) 274
AsuHPI GGTGA 4 cut(s) 118, 323, 524, 611
BaeI ACNNNNGTAYC 2 cut(s) 120, 153
BanI GGYRCC 1 cut(s) 37
BarI GAAGNNNNNNTAC 2 cut(s) 506, 538
BauI CACGAG 1 cut(s) 216
BbsI GAAGAC 1 cut(s) 161
BbvI GCAGC 2 cut(s) 51, 386
BccI CCATC 7 cut(s) 20, 59, 97, 115, 463, 486, 600
BceAI ACGGC 2 cut(s) 25, 261
BciT130I CCWGG 1 cut(s) 597
BcoDI GTCTC 3 cut(s) 7, 108, 413
BcuI ACTAGT 1 cut(s) 182
BfaI CTAG 3 cut(s) 183, 197, 503
BfmI CTRYAG 1 cut(s) 85
BfoI RGCGCY 1 cut(s) 41
BisI GCNGC 4 cut(s) 65, 68, 375, 428
BlsI GCNGC 4 cut(s) 66, 69, 376, 429
Bme1390I CCNGG 1 cut(s) 597
BmgT120I GGNCC 1 cut(s) 274
BmiI GGNNCC 2 cut(s) 39, 283
BmrFI CCNGG 1 cut(s) 597
BmsI GCATC 4 cut(s) 181, 386, 526, 592
BpiI GAAGAC 1 cut(s) 161
BplI GAGNNNNNCTC 1 cut(s) 36
BsaHI GRCGYC 1 cut(s) 38
BsaJI CCNNGG 1 cut(s) 277
Bse1I ACTGG 1 cut(s) 611
Bse3DI GCAATG 3 cut(s) 321, 354, 422
BseBI CCWGG 1 cut(s) 597
BseDI CCNNGG 1 cut(s) 277
BseGI GGATG 1 cut(s) 592
BseMI GCAATG 3 cut(s) 321, 354, 422
BseMII CTCAG 3 cut(s) 123, 246, 359
BseNI ACTGG 1 cut(s) 611
BseXI GCAGC 2 cut(s) 51, 386
BshFI GGCC 2 cut(s) 50, 276
BshNI GGYRCC 1 cut(s) 37
BslFI GGGAC 1 cut(s) 562
BsmAI GTCTC 3 cut(s) 7, 108, 413
BsmFI GGGAC 1 cut(s) 562
BsnI GGCC 2 cut(s) 50, 276
BspACI CCGC 4 cut(s) 68, 206, 326, 427
BspANI GGCC 2 cut(s) 50, 276
BspCNI CTCAG 3 cut(s) 124, 247, 360
BspHI TCATGA 1 cut(s) 22
BspLI GGNNCC 2 cut(s) 39, 283
BspMAI CTGCAG 1 cut(s) 89
BspQI GCTCTTC 1 cut(s) 225
BspT107I GGYRCC 1 cut(s) 37
BsrDI GCAATG 3 cut(s) 321, 354, 422
BsrI ACTGG 1 cut(s) 611
BssECI CCNNGG 1 cut(s) 277
BssNI GRCGYC 1 cut(s) 38
BssSI CACGAG 1 cut(s) 216
Bst2BI CACGAG 1 cut(s) 216
Bst2UI CCWGG 1 cut(s) 597
Bst4CI ACNGT 2 cut(s) 160, 172
Bst6I CTCTTC 3 cut(s) 5, 225, 238
BstACI GRCGYC 1 cut(s) 38
BstDEI CTNAG 4 cut(s) 132, 150, 255, 368
BstDSI CCRYGG 1 cut(s) 277
BstF5I GGATG 1 cut(s) 592
BstH2I RGCGCY 1 cut(s) 41
BstHHI GCGC 1 cut(s) 40
BstMAI GTCTC 3 cut(s) 7, 108, 413
BstMWI GCNNNNNNNGC 1 cut(s) 436
BstNI CCWGG 1 cut(s) 597
BstSCI CCNGG 1 cut(s) 595
BstSFI CTRYAG 1 cut(s) 85
BstV1I GCAGC 2 cut(s) 51, 386
BstV2I GAAGAC 1 cut(s) 161
BsuRI GGCC 2 cut(s) 50, 276
BtgI CCRYGG 1 cut(s) 277
BtsCI GGATG 1 cut(s) 592
BtsIMutI CAGTG 1 cut(s) 252
CciI TCATGA 1 cut(s) 22
CfoI GCGC 1 cut(s) 40
Cfr13I GGNCC 1 cut(s) 274
Csp6I GTAC 2 cut(s) 250, 523
CviAII CATG 2 cut(s) 23, 395
CviJI RGCY 9 cut(s) 50, 64, 235, 276, 282, 393, 430, 439, 622
CviKI_1 RGCY 9 cut(s) 50, 64, 235, 276, 282, 393, 430, 439, 622
CviQI GTAC 2 cut(s) 250, 523
DdeI CTNAG 4 cut(s) 132, 150, 255, 368
DinI GGCGCC 1 cut(s) 39
Eam1104I CTCTTC 3 cut(s) 5, 225, 238
EarI CTCTTC 3 cut(s) 5, 225, 238
EcoRII CCWGG 1 cut(s) 595
EgeI GGCGCC 1 cut(s) 39
EheI GGCGCC 1 cut(s) 39
FaeI CATG 2 cut(s) 26, 398
FaiI YATR 8 cut(s) 24, 288, 396, 413, 528, 557, 566, 589
FaqI GGGAC 1 cut(s) 562
FatI CATG 2 cut(s) 22, 394
Fnu4HI GCNGC 4 cut(s) 65, 68, 375, 428
FokI GGATG 1 cut(s) 579
Fsp4HI GCNGC 4 cut(s) 65, 68, 375, 428
FspBI CTAG 3 cut(s) 183, 197, 503
GlaI GCGC 1 cut(s) 39
GluI GCNGC 4 cut(s) 65, 68, 375, 428
HaeII RGCGCY 1 cut(s) 41
HaeIII GGCC 2 cut(s) 50, 276
HhaI GCGC 1 cut(s) 40
Hin1I GRCGYC 1 cut(s) 38
Hin1II CATG 2 cut(s) 26, 398
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HindIII AAGCTT 1 cut(s) 620
HinfI GANTC 4 cut(s) 19, 259, 317, 575
HphI GGTGA 4 cut(s) 118, 323, 524, 611
Hpy166II GTNNAC 2 cut(s) 211, 252
Hpy188I TCNGA 3 cut(s) 304, 369, 649
Hpy188III TCNNGA 2 cut(s) 23, 240
Hpy8I GTNNAC 2 cut(s) 211, 252
HpyAV CCTTC 1 cut(s) 40
HpyCH4III ACNGT 2 cut(s) 160, 172
HpyCH4V TGCA 4 cut(s) 80, 87, 359, 398
HpyF10VI GCNNNNNNNGC 1 cut(s) 436
HpyF3I CTNAG 4 cut(s) 132, 150, 255, 368
Hsp92I GRCGYC 1 cut(s) 38
Hsp92II CATG 2 cut(s) 26, 398
HspAI GCGC 1 cut(s) 38
KasI GGCGCC 1 cut(s) 37
LguI GCTCTTC 1 cut(s) 225
LmnI GCTCC 2 cut(s) 287, 408
LpnPI CCDG 7 cut(s) 46, 384, 390, 496, 582, 609, 624
Lsp1109I GCAGC 2 cut(s) 51, 386
LweI GCATC 4 cut(s) 181, 386, 526, 592
MaeI CTAG 3 cut(s) 183, 197, 503
MaeIII GTNAC 2 cut(s) 136, 166
MboII GAAGA 6 cut(s) 22, 166, 242, 255, 473, 509
MluCI AATT 1 cut(s) 444
Mly113I GGCGCC 1 cut(s) 38
MlyI GAGTC 3 cut(s) 13, 311, 584
MmeI TCCRAC 1 cut(s) 154
MnlI CCTC 8 cut(s) 25, 127, 214, 239, 302, 304, 590, 643
MseI TTAA 3 cut(s) 225, 443, 456
MslI CAYNNNNRTG 3 cut(s) 269, 483, 597
MspR9I CCNGG 1 cut(s) 597
MvaI CCWGG 1 cut(s) 597
MwoI GCNNNNNNNGC 1 cut(s) 436
NarI GGCGCC 1 cut(s) 38
NlaIII CATG 2 cut(s) 26, 398
NlaIV GGNNCC 2 cut(s) 39, 283
PagI TCATGA 1 cut(s) 22
PciSI GCTCTTC 1 cut(s) 225
PfeI GAWTC 1 cut(s) 259
PkrI GCNGC 4 cut(s) 66, 69, 376, 429
PleI GAGTC 3 cut(s) 13, 311, 583
PluTI GGCGCC 1 cut(s) 41
PpsI GAGTC 3 cut(s) 13, 311, 583
Psp6I CCWGG 1 cut(s) 595
PspGI CCWGG 1 cut(s) 595
PspN4I GGNNCC 2 cut(s) 39, 283
PspPI GGNCC 1 cut(s) 274
PstI CTGCAG 1 cut(s) 89
RsaI GTAC 2 cut(s) 251, 524
RsaNI GTAC 2 cut(s) 250, 523
RseI CAYNNNNRTG 3 cut(s) 269, 483, 597
SapI GCTCTTC 1 cut(s) 225
SaqAI TTAA 3 cut(s) 225, 443, 456
SatI GCNGC 4 cut(s) 65, 68, 375, 428
Sau96I GGNCC 1 cut(s) 274
SchI GAGTC 3 cut(s) 13, 311, 584
ScrFI CCNGG 1 cut(s) 597
SetI ASST 8 cut(s) 36, 138, 225, 237, 313, 601, 624, 635
SfaNI GCATC 4 cut(s) 181, 386, 526, 592
SfcI CTRYAG 1 cut(s) 85
SfoI GGCGCC 1 cut(s) 39
SmiMI CAYNNNNRTG 3 cut(s) 269, 483, 597
SpeI ACTAGT 1 cut(s) 182
Sse9I AATT 1 cut(s) 444
SsiI CCGC 4 cut(s) 68, 206, 326, 427
SspDI GGCGCC 1 cut(s) 37
SspMI CTAG 3 cut(s) 183, 197, 503
StyD4I CCNGG 1 cut(s) 595
TaaI ACNGT 2 cut(s) 160, 172
TasI AATT 1 cut(s) 444
TatI WGTACW 1 cut(s) 522
TauI GCSGC 2 cut(s) 70, 430
TfiI GAWTC 1 cut(s) 259
Tru1I TTAA 3 cut(s) 225, 443, 456
Tru9I TTAA 3 cut(s) 225, 443, 456
TscAI CASTG 1 cut(s) 259
TseI GCWGC 2 cut(s) 64, 374
TspDTI ATGAA 4 cut(s) 158, 336, 474, 581
TspGWI ACGGA 1 cut(s) 321
TspRI CASTG 1 cut(s) 259
XspI CTAG 3 cut(s) 183, 197, 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.