Rroxscaffold_1G00007480

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0016297
Physical Location & Seq
Forward (+)
9566914 .. 9568831
1918 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00007480.1

Sequence Viewer

Length: 1353 bp
ATGGCCGGAAGAAGTACCTATTTGAAGCCATCATCATCTTCGTTGCGCTCTTCATCTGCAATGTTATCAAGTTTGAGTGAGGCATCAACCTCCTGGAATGATTTTGGCTCACACCTGAACAACAACAATGCAGCTAAATCTGCACAGACAGGTCAGCAGCTTCAATGGAGCCAACGCAGTGCAGAACAAGATCTGCAAGAAAATTGTAGCTTTCTAGAAATGCTTGGTGACGGCTGTAACCCATGGAACAAGAGTAGTACACTTCTGAGAAACGAACAACATCTTGATGACGTTAATTATTCTTTGGGAAGTAATGCATTTATGAGAAATGAACAACAGCTTGGAGAGGTTAATTGTCAATCGGGGAACAGTACATCAATGAGAAATGAACAACTGTTTGGTGAGGGTGGTGCTCAATGGGGGAGCAATACATTTCTGAGGAAAGAAAAACATCTTGCCGACAGTAACTTTCCTCTGGAAAACAATACATTTTTTAGGAAAAAACAACAGCTCAATGGGGGTAACTTTCTGTTGGGGAATGGTACATTTATGAGGAAAGAGCAACAGCTGGATGTGGAGAACTTTCACTTGGGGAATAGTACATGTTTGAGTAAAGAGAAACAGCCTGAGAATGGCAACTTCCAAGTGGGAAATAGCACATTTATGAGGAAAGACCAACTTGATGAAGCTAATTTCCAATTTGGGAATAGTACTTTCTTGAGGACAGCACAACAGTTTGATCAGGGTACTTTTCTACTGGGGAAGACTTCATTTATGAGCAAAGAACCACAGCTTGATCGTGGTAATGTTCAAATGGGGAATGGTACATTTATGAGCAAAGAACAACTGCTTAATGAGGGTACTTTCAGACAGGGGAACAGTACCTTTATGAGCAGAGGACAACAACTTGATGAAGGTTCTTTTCAATTGGGGAACAGTACATGTGTGTGGACAGAGCAACAGATTGATGAGGTCAATCACCAATGGTGGAATAGTACATTTTCGAGGACTAGTACATTTTCGAGGAAAGACCACCAATATCTGGGGAGGAGTGAACAACAGCAGCCTGTGGATGAGGGCGTAATGCCTGACTGGTACAGTGCAATATGTTCTGCCAATGAAAGAGCTACCAGTAGTAGTAGTAGTACTCAATGGTACCAAAAGGAGCCTATTGATCAATCTTTGCTGCCCTTGCTCTTGGTGAACAATCAGCAGCCTAACTTGGCTCTTCAGCTGCACGCCCTTATGGCGACATCGTCGGACGAGGCTAGAGGGGACCCTACGTACGGTGTTAATTGGTCCAATCGAAGTTTTGGTTGCAAGATGATGACGTTGTTGTCTTGGGCGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

450

Amino Acids

50.63

Weight (kDa)

5.41

Isoelectric Point (pI)

42.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016297)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g41110
malus_domestica MD03G1047400.v1.1 MD11G1049900.v1.1
pyrus_communis pycom11g04050
rosa_chinensis RchiOBHm_Chr5g0073891
rosa_laevigata RLG00000036422
rosa_multiflora Rmu_co8433661.1_g000001 Rmu_sc0000470.1_g000015
rosa_roxburghii Rroxscaffold_1G00007480
rosa_rugosa Rorug05G0427100
rosa_samantha Rh5AG484100 Rh5BG504800 Rh5CG528600 Rh5DG517100
rosa_wichuraiana Rw5G045000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1336
Acc65I GGTACC 1 cut(s) 1155
AccB1I GGYRCC 1 cut(s) 1155
AccB7I CCANNNNNTGG 1 cut(s) 1042
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 1214
AfiI CCNNNNNNNGG 3 cut(s) 632, 1042, 1286
AflIII ACRYGT 2 cut(s) 602, 941
AgsI TTSAA 4 cut(s) 25, 164, 812, 926
AhlI ACTAGT 1 cut(s) 1010
AjnI CCWGG 1 cut(s) 92
AjuI GAANNNNNNNTTGG 6 cut(s) 324, 356, 381, 413, 572, 604
Alw21I GWGCWC 1 cut(s) 415
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 6 cut(s) 131, 157, 1063, 1186, 1213, 1234
Asp718I GGTACC 1 cut(s) 1155
AspLEI GCGC 1 cut(s) 48
AspS9I GGNCC 2 cut(s) 1276, 1299
AsuHPI GGTGA 4 cut(s) 239, 413, 971, 1213
AvaII GGWCC 2 cut(s) 1276, 1299
BanI GGYRCC 1 cut(s) 1155
BbsI GAAGAC 1 cut(s) 770
Bbv12I GWGCWC 1 cut(s) 415
BbvI GCAGC 6 cut(s) 143, 169, 1075, 1173, 1221, 1225
BccI CCATC 1 cut(s) 37
BceAI ACGGC 1 cut(s) 247
BciT130I CCWGG 1 cut(s) 94
BclI TGATCA 2 cut(s) 739, 1174
BcuI ACTAGT 1 cut(s) 1010
BfaI CTAG 3 cut(s) 215, 1011, 1269
BglI GCCNNNNNGGC 1 cut(s) 1247
BglII AGATCT 1 cut(s) 190
BisI GCNGC 6 cut(s) 132, 158, 1064, 1187, 1214, 1235
BlsI GCNGC 6 cut(s) 133, 159, 1065, 1188, 1215, 1236
BmcAI AGTACT 2 cut(s) 712, 1147
Bme1390I CCNGG 1 cut(s) 94
Bme18I GGWCC 2 cut(s) 1276, 1299
BmgT120I GGNCC 2 cut(s) 1276, 1299
BmiI GGNNCC 5 cut(s) 170, 1157, 1167, 1277, 1278
BmrFI CCNGG 1 cut(s) 94
BmrI ACTGGG 1 cut(s) 767
BmsI GCATC 1 cut(s) 92
BmuI ACTGGG 1 cut(s) 767
BpiI GAAGAC 1 cut(s) 770
BpuEI CTTGAG 1 cut(s) 739
BsaAI YACGTR 1 cut(s) 1284
BsaJI CCNNGG 1 cut(s) 242
Bsc4I CCNNNNNNNGG 3 cut(s) 632, 1042, 1286
Bse1I ACTGG 3 cut(s) 762, 1097, 1131
Bse3DI GCAATG 1 cut(s) 66
BseBI CCWGG 1 cut(s) 94
BseDI CCNNGG 1 cut(s) 242
BseGI GGATG 2 cut(s) 577, 1078
BseLI CCNNNNNNNGG 3 cut(s) 632, 1042, 1286
BseMI GCAATG 1 cut(s) 66
BseMII CTCAG 3 cut(s) 257, 428, 618
BseNI ACTGG 3 cut(s) 762, 1097, 1131
BseRI GAGGAG 1 cut(s) 1063
BseXI GCAGC 6 cut(s) 143, 169, 1075, 1173, 1221, 1225
BsgI GTGCAG 3 cut(s) 126, 201, 1220
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 1155
BsiHKAI GWGCWC 1 cut(s) 415
BsiSI CCGG 1 cut(s) 6
BsiWI CGTACG 1 cut(s) 1284
BslFI GGGAC 1 cut(s) 1289
BslI CCNNNNNNNGG 3 cut(s) 632, 1042, 1286
BsmFI GGGAC 1 cut(s) 1289
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 415
Bsp143I GATC 4 cut(s) 190, 739, 796, 1174
Bsp19I CCATGG 1 cut(s) 242
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 3 cut(s) 258, 429, 619
BspLI GGNNCC 5 cut(s) 170, 1157, 1167, 1277, 1278
BspQI GCTCTTC 2 cut(s) 55, 1233
BspT107I GGYRCC 1 cut(s) 1155
BsrDI GCAATG 1 cut(s) 66
BsrI ACTGG 3 cut(s) 762, 1097, 1131
BssECI CCNNGG 1 cut(s) 242
BssMI GATC 4 cut(s) 190, 739, 796, 1174
BssT1I CCWWGG 1 cut(s) 242
Bst2UI CCWGG 1 cut(s) 94
Bst4CI ACNGT 8 cut(s) 371, 396, 464, 735, 881, 938, 1100, 1289
Bst6I CTCTTC 2 cut(s) 55, 1233
BstBAI YACGTR 1 cut(s) 1284
BstC8I GCNNGC 1 cut(s) 1239
BstDEI CTNAG 3 cut(s) 266, 437, 627
BstDSI CCRYGG 1 cut(s) 242
BstF5I GGATG 2 cut(s) 577, 1078
BstHHI GCGC 1 cut(s) 48
BstKTI GATC 4 cut(s) 193, 742, 799, 1177
BstMBI GATC 4 cut(s) 190, 739, 796, 1174
BstMWI GCNNNNNNNGC 3 cut(s) 140, 1192, 1247
BstNI CCWGG 1 cut(s) 94
BstNSI RCATGY 2 cut(s) 606, 945
BstSCI CCNGG 1 cut(s) 92
BstSNI TACGTA 1 cut(s) 1284
BstV1I GCAGC 6 cut(s) 143, 169, 1075, 1173, 1221, 1225
BstV2I GAAGAC 1 cut(s) 770
BstX2I RGATCY 1 cut(s) 190
BstYI RGATCY 1 cut(s) 190
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 242
BtsCI GGATG 2 cut(s) 577, 1078
BtsI GCAGTG 1 cut(s) 184
BtsIMutI CAGTG 2 cut(s) 184, 1105
Cac8I GCNNGC 1 cut(s) 1239
CfoI GCGC 1 cut(s) 48
Cfr13I GGNCC 2 cut(s) 1276, 1299
CviAII CATG 3 cut(s) 243, 603, 942
DdeI CTNAG 3 cut(s) 266, 437, 627
DpnI GATC 4 cut(s) 192, 741, 798, 1176
DpnII GATC 4 cut(s) 190, 739, 796, 1174
DrdI GACNNNNNNGTC 1 cut(s) 1336
DseDI GACNNNNNNGTC 1 cut(s) 1336
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 55, 1233
EarI CTCTTC 2 cut(s) 55, 1233
Eco105I TACGTA 1 cut(s) 1284
Eco130I CCWWGG 1 cut(s) 242
Eco47I GGWCC 2 cut(s) 1276, 1299
Eco57I CTGAAG 1 cut(s) 1214
EcoO109I RGGNCCY 1 cut(s) 1276
EcoRII CCWGG 1 cut(s) 92
EcoT14I CCWWGG 1 cut(s) 242
EcoT22I ATGCAT 1 cut(s) 319
ErhI CCWWGG 1 cut(s) 242
FaeI CATG 3 cut(s) 246, 606, 945
FalI AAGNNNNNCTT 2 cut(s) 663, 695
FaqI GGGAC 1 cut(s) 1289
FatI CATG 3 cut(s) 242, 602, 941
FbaI TGATCA 2 cut(s) 739, 1174
Fnu4HI GCNGC 6 cut(s) 132, 158, 1064, 1187, 1214, 1235
FokI GGATG 2 cut(s) 584, 1085
Fsp4HI GCNGC 6 cut(s) 132, 158, 1064, 1187, 1214, 1235
FspBI CTAG 3 cut(s) 215, 1011, 1269
GlaI GCGC 1 cut(s) 47
GluI GCNGC 6 cut(s) 132, 158, 1064, 1187, 1214, 1235
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 6
HhaI GCGC 1 cut(s) 48
Hin1II CATG 3 cut(s) 246, 606, 945
Hin6I GCGC 1 cut(s) 46
HinP1I GCGC 1 cut(s) 46
HpaII CCGG 1 cut(s) 6
HphI GGTGA 4 cut(s) 239, 413, 971, 1213
Hpy166II GTNNAC 4 cut(s) 260, 951, 1055, 1204
Hpy188I TCNGA 4 cut(s) 267, 438, 869, 1261
Hpy188III TCNNGA 4 cut(s) 215, 284, 476, 718
Hpy8I GTNNAC 4 cut(s) 260, 951, 1055, 1204
Hpy99I CGWCG 1 cut(s) 1261
HpyAV CCTTC 1 cut(s) 908
HpyCH4III ACNGT 8 cut(s) 371, 396, 464, 735, 881, 938, 1100, 1289
HpyCH4IV ACGT 3 cut(s) 291, 1283, 1331
HpyCH4V TGCA 9 cut(s) 59, 131, 143, 182, 196, 317, 1103, 1237, 1320
HpyF10VI GCNNNNNNNGC 3 cut(s) 140, 1192, 1247
HpyF3I CTNAG 3 cut(s) 266, 437, 627
HpySE526I ACGT 3 cut(s) 291, 1283, 1331
Hsp92II CATG 3 cut(s) 246, 606, 945
HspAI GCGC 1 cut(s) 46
KflI GGGWCCC 1 cut(s) 1276
KpnI GGTACC 1 cut(s) 1159
Ksp22I TGATCA 2 cut(s) 739, 1174
Kzo9I GATC 4 cut(s) 190, 739, 796, 1174
LguI GCTCTTC 2 cut(s) 55, 1233
LmnI GCTCC 3 cut(s) 168, 423, 1165
Lsp1109I GCAGC 6 cut(s) 143, 169, 1075, 1173, 1221, 1225
LweI GCATC 1 cut(s) 92
MaeI CTAG 3 cut(s) 215, 1011, 1269
MaeII ACGT 3 cut(s) 291, 1283, 1331
MaeIII GTNAC 4 cut(s) 227, 236, 464, 521
MalI GATC 4 cut(s) 192, 741, 798, 1176
MboI GATC 4 cut(s) 190, 739, 796, 1174
MboII GAAGA 5 cut(s) 21, 30, 42, 775, 1220
MfeI CAATTG 1 cut(s) 926
MflI RGATCY 1 cut(s) 190
MhlI GDGCHC 1 cut(s) 415
MluCI AATT 7 cut(s) 202, 295, 352, 691, 698, 926, 1294
MmeI TCCRAC 1 cut(s) 1239
Mph1103I ATGCAT 1 cut(s) 319
MseI TTAA 4 cut(s) 294, 351, 852, 1293
MslI CAYNNNNRTG 3 cut(s) 285, 662, 946
MspA1I CMGCKG 2 cut(s) 568, 1234
MspI CCGG 1 cut(s) 6
MspR9I CCNGG 1 cut(s) 94
MunI CAATTG 1 cut(s) 926
MvaI CCWGG 1 cut(s) 94
MwoI GCNNNNNNNGC 3 cut(s) 140, 1192, 1247
NcoI CCATGG 1 cut(s) 242
NdeII GATC 4 cut(s) 190, 739, 796, 1174
NlaIII CATG 3 cut(s) 246, 606, 945
NlaIV GGNNCC 5 cut(s) 170, 1157, 1167, 1277, 1278
NmuCI GTSAC 1 cut(s) 227
NsiI ATGCAT 1 cut(s) 319
NspI RCATGY 2 cut(s) 606, 945
PciI ACATGT 2 cut(s) 602, 941
PciSI GCTCTTC 2 cut(s) 55, 1233
Pfl23II CGTACG 1 cut(s) 1284
PflFI GACNNNGTC 1 cut(s) 1255
PflMI CCANNNNNTGG 1 cut(s) 1042
PfoI TCCNGGA 1 cut(s) 92
PkrI GCNGC 6 cut(s) 133, 159, 1065, 1188, 1215, 1236
Ppu21I YACGTR 1 cut(s) 1284
PpuMI RGGWCCY 1 cut(s) 1276
PscI ACATGT 2 cut(s) 602, 941
Psp5II RGGWCCY 1 cut(s) 1276
Psp6I CCWGG 1 cut(s) 92
PspGI CCWGG 1 cut(s) 92
PspLI CGTACG 1 cut(s) 1284
PspN4I GGNNCC 5 cut(s) 170, 1157, 1167, 1277, 1278
PspPI GGNCC 2 cut(s) 1276, 1299
PspPPI RGGWCCY 1 cut(s) 1276
PsuI RGATCY 1 cut(s) 190
PsyI GACNNNGTC 1 cut(s) 1255
PvuII CAGCTG 2 cut(s) 568, 1234
RseI CAYNNNNRTG 3 cut(s) 285, 662, 946
SapI GCTCTTC 2 cut(s) 55, 1233
SaqAI TTAA 4 cut(s) 294, 351, 852, 1293
SatI GCNGC 6 cut(s) 132, 158, 1064, 1187, 1214, 1235
Sau3AI GATC 4 cut(s) 190, 739, 796, 1174
Sau96I GGNCC 2 cut(s) 1276, 1299
ScaI AGTACT 2 cut(s) 712, 1147
ScrFI CCNGG 1 cut(s) 94
SduI GDGCHC 1 cut(s) 415
SfaNI GCATC 1 cut(s) 92
SinI GGWCC 2 cut(s) 1276, 1299
SmiMI CAYNNNNRTG 3 cut(s) 285, 662, 946
SmlI CTYRAG 1 cut(s) 718
SmoI CTYRAG 1 cut(s) 718
SnaBI TACGTA 1 cut(s) 1284
SpeI ACTAGT 1 cut(s) 1010
Sse9I AATT 7 cut(s) 202, 295, 352, 691, 698, 926, 1294
SspMI CTAG 3 cut(s) 215, 1011, 1269
StyD4I CCNGG 1 cut(s) 92
StyI CCWWGG 1 cut(s) 242
TaaI ACNGT 8 cut(s) 371, 396, 464, 735, 881, 938, 1100, 1289
TaiI ACGT 3 cut(s) 294, 1286, 1334
TaqI TCGA 3 cut(s) 1004, 1022, 1306
TasI AATT 7 cut(s) 202, 295, 352, 691, 698, 926, 1294
TatI WGTACW 8 cut(s) 257, 371, 599, 710, 938, 995, 1013, 1145
Tru1I TTAA 4 cut(s) 294, 351, 852, 1293
Tru9I TTAA 4 cut(s) 294, 351, 852, 1293
TscAI CASTG 2 cut(s) 184, 1105
TseFI GTSAC 1 cut(s) 227
TseI GCWGC 6 cut(s) 131, 157, 1063, 1186, 1213, 1234
Tsp45I GTSAC 1 cut(s) 227
TspDTI ATGAA 7 cut(s) 42, 345, 402, 699, 759, 927, 1134
TspRI CASTG 2 cut(s) 184, 1105
Tth111I GACNNNGTC 1 cut(s) 1255
Van91I CCANNNNNTGG 1 cut(s) 1042
VpaK11BI GGWCC 2 cut(s) 1276, 1299
XbaI TCTAGA 1 cut(s) 214
XceI RCATGY 2 cut(s) 606, 945
XspI CTAG 3 cut(s) 215, 1011, 1269
ZrmI AGTACT 2 cut(s) 712, 1147
Zsp2I ATGCAT 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.