Rroxscaffold_1G00020680

Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0003760
Physical Location & Seq
Reverse (-)
25160035 .. 25160908
874 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00020680.1

Sequence Viewer

Length: 627 bp
ATGGAGGAGATGGTGGTGCTTCGGTTATCCACCAAAGTGAAACAAGAAAGTAGCCCATACCAGATCGGGGGGAGGTGCACACAATTAATCGCAGAATCTCTGTCACTCATGCTTGTCCCCGAGGAGGAACATCCCAATGCCATCAACAAGTTACTTGGAGGACTCGACTCGGAGAGACCAGCAAGTGTGCAGTGCAGTTCCGCAGCTCTTCCGGGGCTGCCGTACCTAGAGATGGCGAGGAAGATGCTAGTTGATGGTGAGCCGAGCAAACCGAATGAAGAGGAGGCCCACTATGACTTCGACTTGTTTGTTATTGGGGCTGGAAGCGGTGGCGTTCGTGCCTCCGCATTTGCAGCTAATTTCGGAGCTAAGGTTGCCATATGTGAGCTTCCATTTCATCCTATCGATTCCGAAGTGATTGGAGGAGTTGGTGGAACATGTGTCATTCGTGGTTGTGTTCCCAAAAAGATATTGGTATATGGAGCATCCTTTGGCGGTGAAATTCAGGATGCCAAGAATTATGGGTGGGAAGTGAATGACGAGTTGATTTTAACTCGGAAGAAACTTTTGCAGAAACAATTGGGTAATCTTCGTACCCTTTTCGGTTTGGAAATTGAATTGTTGTGA

Protein Analysis

208

Amino Acids

22.48

Weight (kDa)

5.09

Isoelectric Point (pI)

52.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_2 PF07992 100 - 181 1.8e-08 Pyridine nucleotide-disulphide oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 201, 327, 345, 495
AcsI RAATTY 1 cut(s) 501
AfaI GTAC 2 cut(s) 224, 595
AfiI CCNNNNNNNGG 3 cut(s) 67, 124, 232
AflIII ACRYGT 1 cut(s) 437
AgsI TTSAA 1 cut(s) 617
AleI CACNNNNGTG 1 cut(s) 35
AluBI AGCT 4 cut(s) 206, 356, 368, 388
AluI AGCT 4 cut(s) 206, 356, 368, 388
Alw21I GWGCWC 1 cut(s) 80
Alw26I GTCTC 1 cut(s) 169
Alw44I GTGCAC 1 cut(s) 76
Ama87I CYCGRG 1 cut(s) 119
AoxI GGCC 1 cut(s) 285
ApaLI GTGCAC 1 cut(s) 76
ApeKI GCWGC 3 cut(s) 203, 217, 353
ApoI RAATTY 1 cut(s) 501
AseI ATTAAT 1 cut(s) 86
AspS9I GGNCC 1 cut(s) 286
AsuC2I CCSGG 1 cut(s) 213
AsuHPI GGTGA 2 cut(s) 269, 509
AvaI CYCGRG 1 cut(s) 119
BaeGI GKGCMC 1 cut(s) 80
Bbv12I GWGCWC 1 cut(s) 80
BbvI GCAGC 3 cut(s) 204, 215, 365
BccI CCATC 4 cut(s) 4, 149, 226, 248
BceAI ACGGC 1 cut(s) 205
BcgI CGANNNNNNTGC 2 cut(s) 226, 260
BcnI CCSGG 1 cut(s) 213
BcoDI GTCTC 1 cut(s) 169
BfaI CTAG 2 cut(s) 227, 248
BisI GCNGC 3 cut(s) 204, 218, 354
BlsI GCNGC 3 cut(s) 205, 219, 355
Bme1390I CCNGG 1 cut(s) 213
BmeT110I CYCGRG 1 cut(s) 119
BmgT120I GGNCC 1 cut(s) 286
BmrFI CCNGG 1 cut(s) 213
BmsI GCATC 3 cut(s) 234, 494, 499
Bpu10I CCTNAGC 1 cut(s) 369
BpuMI CCSGG 1 cut(s) 213
Bsa29I ATCGAT 1 cut(s) 405
BsaI GGTCTC 1 cut(s) 169
BsaJI CCNNGG 2 cut(s) 120, 212
BsaXI ACNNNNNCTCC 2 cut(s) 357, 387
Bsc4I CCNNNNNNNGG 3 cut(s) 67, 124, 232
BseCI ATCGAT 1 cut(s) 405
BseDI CCNNGG 2 cut(s) 120, 212
BseGI GGATG 4 cut(s) 130, 397, 485, 514
BseLI CCNNNNNNNGG 3 cut(s) 67, 124, 232
BseRI GAGGAG 4 cut(s) 20, 137, 296, 438
BseSI GKGCMC 1 cut(s) 80
BseXI GCAGC 3 cut(s) 204, 215, 365
BsgI GTGCAG 2 cut(s) 209, 214
BshFI GGCC 1 cut(s) 287
BshVI ATCGAT 1 cut(s) 405
BsiHKAI GWGCWC 1 cut(s) 80
BsiHKCI CYCGRG 1 cut(s) 119
BsiSI CCGG 1 cut(s) 212
BslFI GGGAC 1 cut(s) 101
BslI CCNNNNNNNGG 3 cut(s) 67, 124, 232
BsmAI GTCTC 1 cut(s) 169
BsmFI GGGAC 1 cut(s) 101
BsnI GGCC 1 cut(s) 287
Bso31I GGTCTC 1 cut(s) 169
BsoBI CYCGRG 1 cut(s) 119
Bsp1286I GDGCHC 1 cut(s) 80
Bsp143I GATC 1 cut(s) 63
BspACI CCGC 4 cut(s) 201, 327, 345, 495
BspANI GGCC 1 cut(s) 287
BspDI ATCGAT 1 cut(s) 405
BspQI GCTCTTC 1 cut(s) 213
BspTNI GGTCTC 1 cut(s) 169
BssECI CCNNGG 2 cut(s) 120, 212
BssMI GATC 1 cut(s) 63
Bst6I CTCTTC 2 cut(s) 213, 273
BstDEI CTNAG 1 cut(s) 369
BstF5I GGATG 4 cut(s) 130, 397, 485, 514
BstKTI GATC 1 cut(s) 66
BstMAI GTCTC 1 cut(s) 169
BstMBI GATC 1 cut(s) 63
BstMWI GCNNNNNNNGC 2 cut(s) 353, 374
BstNSI RCATGY 1 cut(s) 441
BstSCI CCNGG 1 cut(s) 211
BstSLI GKGCMC 1 cut(s) 80
BstV1I GCAGC 3 cut(s) 204, 215, 365
Bsu15I ATCGAT 1 cut(s) 405
BsuRI GGCC 1 cut(s) 287
BsuTUI ATCGAT 1 cut(s) 405
BtsCI GGATG 4 cut(s) 130, 397, 485, 514
BtsI GCAGTG 1 cut(s) 197
BtsIMutI CAGTG 1 cut(s) 197
Cfr13I GGNCC 1 cut(s) 286
ClaI ATCGAT 1 cut(s) 405
Csp6I GTAC 2 cut(s) 223, 594
CviAII CATG 2 cut(s) 109, 438
CviJI RGCY 9 cut(s) 54, 206, 217, 262, 287, 320, 356, 368, 388
CviKI_1 RGCY 9 cut(s) 54, 206, 217, 262, 287, 320, 356, 368, 388
CviQI GTAC 2 cut(s) 223, 594
DdeI CTNAG 1 cut(s) 369
DpnI GATC 1 cut(s) 65
DpnII GATC 1 cut(s) 63
Eam1104I CTCTTC 2 cut(s) 213, 273
EarI CTCTTC 2 cut(s) 213, 273
Eco31I GGTCTC 1 cut(s) 169
Eco88I CYCGRG 1 cut(s) 119
FaeI CATG 2 cut(s) 112, 441
FaiI YATR 9 cut(s) 58, 110, 294, 380, 382, 439, 478, 480, 522
FaqI GGGAC 1 cut(s) 101
FatI CATG 2 cut(s) 108, 437
FauNDI CATATG 1 cut(s) 380
Fnu4HI GCNGC 3 cut(s) 204, 218, 354
FokI GGATG 4 cut(s) 117, 384, 472, 521
Fsp4HI GCNGC 3 cut(s) 204, 218, 354
FspBI CTAG 2 cut(s) 227, 248
GluI GCNGC 3 cut(s) 204, 218, 354
HaeIII GGCC 1 cut(s) 287
HapII CCGG 1 cut(s) 212
Hin1II CATG 2 cut(s) 112, 441
HinfI GANTC 4 cut(s) 95, 162, 167, 407
HpaII CCGG 1 cut(s) 212
HphI GGTGA 2 cut(s) 269, 509
Hpy166II GTNNAC 1 cut(s) 78
Hpy188I TCNGA 4 cut(s) 172, 365, 412, 558
Hpy188III TCNNGA 1 cut(s) 506
Hpy8I GTNNAC 1 cut(s) 78
HpyCH4V TGCA 5 cut(s) 78, 190, 195, 353, 571
HpyF10VI GCNNNNNNNGC 2 cut(s) 353, 374
HpyF3I CTNAG 1 cut(s) 369
Hsp92II CATG 2 cut(s) 112, 441
Kzo9I GATC 1 cut(s) 63
LguI GCTCTTC 1 cut(s) 213
LmnI GCTCC 2 cut(s) 365, 482
LpnPI CCDG 5 cut(s) 74, 192, 225, 306, 491
Lsp1109I GCAGC 3 cut(s) 204, 215, 365
LweI GCATC 3 cut(s) 234, 494, 499
MaeI CTAG 2 cut(s) 227, 248
MaeIII GTNAC 2 cut(s) 102, 150
MalI GATC 1 cut(s) 65
MboI GATC 1 cut(s) 63
MboII GAAGA 5 cut(s) 200, 253, 290, 571, 581
MfeI CAATTG 1 cut(s) 578
MhlI GDGCHC 1 cut(s) 80
MluCI AATT 7 cut(s) 83, 358, 501, 517, 578, 612, 617
MlyI GAGTC 2 cut(s) 156, 161
MnlI CCTC 9 cut(s) 66, 115, 118, 152, 231, 274, 277, 352, 416
MseI TTAA 2 cut(s) 86, 551
MslI CAYNNNNRTG 2 cut(s) 35, 135
MspI CCGG 1 cut(s) 212
MspR9I CCNGG 1 cut(s) 213
MunI CAATTG 1 cut(s) 578
MwoI GCNNNNNNNGC 2 cut(s) 353, 374
NciI CCSGG 1 cut(s) 213
NdeI CATATG 1 cut(s) 380
NdeII GATC 1 cut(s) 63
NlaIII CATG 2 cut(s) 112, 441
NmeAIII GCCGAG 1 cut(s) 288
NmuCI GTSAC 1 cut(s) 102
NspI RCATGY 1 cut(s) 441
OliI CACNNNNGTG 1 cut(s) 35
PciI ACATGT 1 cut(s) 437
PciSI GCTCTTC 1 cut(s) 213
PfeI GAWTC 2 cut(s) 95, 407
PkrI GCNGC 3 cut(s) 205, 219, 355
PleI GAGTC 2 cut(s) 156, 161
PpsI GAGTC 2 cut(s) 156, 161
PscI ACATGT 1 cut(s) 437
PshBI ATTAAT 1 cut(s) 86
PspPI GGNCC 1 cut(s) 286
RsaI GTAC 2 cut(s) 224, 595
RsaNI GTAC 2 cut(s) 223, 594
RseI CAYNNNNRTG 2 cut(s) 35, 135
SapI GCTCTTC 1 cut(s) 213
SaqAI TTAA 2 cut(s) 86, 551
SatI GCNGC 3 cut(s) 204, 218, 354
Sau3AI GATC 1 cut(s) 63
Sau96I GGNCC 1 cut(s) 286
SchI GAGTC 2 cut(s) 156, 161
ScrFI CCNGG 1 cut(s) 213
SduI GDGCHC 1 cut(s) 80
SetI ASST 7 cut(s) 77, 208, 228, 358, 370, 375, 390
SfaNI GCATC 3 cut(s) 234, 494, 499
SmiMI CAYNNNNRTG 2 cut(s) 35, 135
Sse9I AATT 7 cut(s) 83, 358, 501, 517, 578, 612, 617
SsiI CCGC 4 cut(s) 201, 327, 345, 495
SspMI CTAG 2 cut(s) 227, 248
StyD4I CCNGG 1 cut(s) 211
TaqI TCGA 3 cut(s) 165, 300, 405
TasI AATT 7 cut(s) 83, 358, 501, 517, 578, 612, 617
TfiI GAWTC 2 cut(s) 95, 407
Tru1I TTAA 2 cut(s) 86, 551
Tru9I TTAA 2 cut(s) 86, 551
TscAI CASTG 1 cut(s) 197
TseFI GTSAC 1 cut(s) 102
TseI GCWGC 3 cut(s) 203, 217, 353
Tsp45I GTSAC 1 cut(s) 102
TspDTI ATGAA 2 cut(s) 291, 386
TspRI CASTG 1 cut(s) 197
VneI GTGCAC 1 cut(s) 76
VspI ATTAAT 1 cut(s) 86
XapI RAATTY 1 cut(s) 501
XceI RCATGY 1 cut(s) 441
XcmI CCANNNNNNNNNTGG 1 cut(s) 469
XspI CTAG 2 cut(s) 227, 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.