Rroxscaffold_1G00028590

Protein of unknown function, DUF604

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005378
Physical Location & Seq
Reverse (-)
36432282 .. 36432824
543 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00028590.1

Sequence Viewer

Length: 543 bp
ATGAACCGCTCTGACTCCATTAACCACCTCATGAAAGCTGCAAAAGTTGATCCCTCTCGTCTTCTTCAACAAACCATATGCTACCACAGACAAAGCAATTGGTCTTTCTCAATCTCATGGGGTTATTCTGCTCACATTTATGAAAACATAATCCCCCGGAGCACTTTACGGAAGCCCATTGAGACATTCTCGCGTTGGAGGAACGCGAGCCGACCATTCTACATGTTCAACACCAGGTTGCCCTCCAAGGATCCATGTCGAGCTCCCCATGTGTTTTATTTTGATTCTATTGATCATCGTGTCGATGGAAGGAAGACTAAGACTGTTACAACTTATTCAAGAGTAGCTCCGCGTGGTTTGGTGTCATGTTCTTCAAATGGTAACCACTCTGCGGATTCCATCACCAAAATTGAGGTCTATACATCGCCAGAATTCCGTTTGGAGATGAAAGGAAAGGACTGCTGTGACATAGTACATGAAGCTGATATTCACACTACTCGAGTCATATACAGGTCTTGCATGGAGGGTGAAATTATCGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF604

Protein Analysis

180

Amino Acids

20.85

Weight (kDa)

9.32

Isoelectric Point (pI)

59.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF604 PF04646 1 - 145 4.4e-44 Protein of unknown function, DUF604
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 9
AccII CGCG 3 cut(s) 193, 206, 352
AciI CCGC 3 cut(s) 7, 350, 392
AclWI GGATC 3 cut(s) 44, 245, 258
AcsI RAATTY 1 cut(s) 431
AfaI GTAC 1 cut(s) 474
AfiI CCNNNNNNNGG 1 cut(s) 391
AflIII ACRYGT 1 cut(s) 222
AgsI TTSAA 4 cut(s) 68, 229, 339, 375
AjnI CCWGG 1 cut(s) 233
AluBI AGCT 4 cut(s) 38, 263, 347, 482
AluI AGCT 4 cut(s) 38, 263, 347, 482
Alw21I GWGCWC 2 cut(s) 164, 265
Alw26I GTCTC 1 cut(s) 176
AlwI GGATC 3 cut(s) 44, 245, 258
Ama87I CYCGRG 1 cut(s) 498
ApeKI GCWGC 1 cut(s) 38
ApoI RAATTY 1 cut(s) 431
ArsI GACNNNNNNTTYG 2 cut(s) 399, 431
AsuC2I CCSGG 1 cut(s) 157
AsuHPI GGTGA 2 cut(s) 394, 539
AvaI CYCGRG 1 cut(s) 498
BamHI GGATCC 1 cut(s) 250
BanII GRGCYC 1 cut(s) 265
BbsI GAAGAC 2 cut(s) 53, 320
Bbv12I GWGCWC 2 cut(s) 164, 265
BbvI GCAGC 1 cut(s) 25
BccI CCATC 2 cut(s) 299, 407
BciT130I CCWGG 1 cut(s) 235
BclI TGATCA 1 cut(s) 292
BcnI CCSGG 1 cut(s) 157
BcoDI GTCTC 1 cut(s) 176
BisI GCNGC 1 cut(s) 39
BlsI GCNGC 1 cut(s) 40
Bme1390I CCNGG 2 cut(s) 157, 235
BmeT110I CYCGRG 1 cut(s) 498
BmiI GGNNCC 1 cut(s) 252
BmrFI CCNGG 2 cut(s) 157, 235
BpiI GAAGAC 2 cut(s) 53, 320
BplI GAGNNNNNCTC 2 cut(s) 173, 205
BpuMI CCSGG 1 cut(s) 157
BsaJI CCNNGG 2 cut(s) 155, 246
Bsc4I CCNNNNNNNGG 1 cut(s) 391
BseBI CCWGG 1 cut(s) 235
BseDI CCNNGG 2 cut(s) 155, 246
BseLI CCNNNNNNNGG 1 cut(s) 391
BseXI GCAGC 1 cut(s) 25
Bsh1236I CGCG 3 cut(s) 193, 206, 352
BsiHKAI GWGCWC 2 cut(s) 164, 265
BsiHKCI CYCGRG 1 cut(s) 498
BsiSI CCGG 1 cut(s) 157
BslI CCNNNNNNNGG 1 cut(s) 391
BsmAI GTCTC 1 cut(s) 176
BsoBI CYCGRG 1 cut(s) 498
Bsp1286I GDGCHC 2 cut(s) 164, 265
Bsp143I GATC 3 cut(s) 49, 250, 292
BspACI CCGC 3 cut(s) 7, 350, 392
BspFNI CGCG 3 cut(s) 193, 206, 352
BspHI TCATGA 1 cut(s) 30
BspLI GGNNCC 1 cut(s) 252
BspPI GGATC 3 cut(s) 44, 245, 258
BsrBI CCGCTC 1 cut(s) 9
BssECI CCNNGG 2 cut(s) 155, 246
BssMI GATC 3 cut(s) 49, 250, 292
BssT1I CCWWGG 1 cut(s) 246
Bst2UI CCWGG 1 cut(s) 235
Bst4CI ACNGT 1 cut(s) 325
BstC8I GCNNGC 1 cut(s) 208
BstDEI CTNAG 1 cut(s) 318
BstEII GGTNACC 1 cut(s) 380
BstFNI CGCG 3 cut(s) 193, 206, 352
BstKTI GATC 3 cut(s) 52, 253, 295
BstMAI GTCTC 1 cut(s) 176
BstMBI GATC 3 cut(s) 49, 250, 292
BstNI CCWGG 1 cut(s) 235
BstNSI RCATGY 1 cut(s) 226
BstPI GGTNACC 1 cut(s) 380
BstSCI CCNGG 2 cut(s) 155, 233
BstUI CGCG 3 cut(s) 193, 206, 352
BstV1I GCAGC 1 cut(s) 25
BstV2I GAAGAC 2 cut(s) 53, 320
BstX2I RGATCY 1 cut(s) 250
BstYI RGATCY 1 cut(s) 250
BtgZI GCGATG 1 cut(s) 408
Cac8I GCNNGC 1 cut(s) 208
CciI TCATGA 1 cut(s) 30
CsiI ACCWGGT 1 cut(s) 233
Csp6I GTAC 1 cut(s) 473
CviAII CATG 8 cut(s) 31, 117, 223, 255, 269, 366, 476, 520
CviJI RGCY 6 cut(s) 38, 175, 210, 263, 347, 482
CviKI_1 RGCY 6 cut(s) 38, 175, 210, 263, 347, 482
CviQI GTAC 1 cut(s) 473
DdeI CTNAG 1 cut(s) 318
DpnI GATC 3 cut(s) 51, 252, 294
DpnII GATC 3 cut(s) 49, 250, 292
Ecl136II GAGCTC 1 cut(s) 263
Eco130I CCWWGG 1 cut(s) 246
Eco24I GRGCYC 1 cut(s) 265
Eco53kI GAGCTC 1 cut(s) 263
Eco88I CYCGRG 1 cut(s) 498
Eco91I GGTNACC 1 cut(s) 380
EcoICRI GAGCTC 1 cut(s) 263
EcoO65I GGTNACC 1 cut(s) 380
EcoRI GAATTC 1 cut(s) 431
EcoRII CCWGG 1 cut(s) 233
EcoT14I CCWWGG 1 cut(s) 246
EcoT38I GRGCYC 1 cut(s) 265
ErhI CCWWGG 1 cut(s) 246
FaeI CATG 8 cut(s) 34, 120, 226, 258, 272, 369, 479, 523
FatI CATG 8 cut(s) 30, 116, 222, 254, 268, 365, 475, 519
FauNDI CATATG 1 cut(s) 77
FbaI TGATCA 1 cut(s) 292
Fnu4HI GCNGC 1 cut(s) 39
FriOI GRGCYC 1 cut(s) 265
Fsp4HI GCNGC 1 cut(s) 39
GluI GCNGC 1 cut(s) 39
HapII CCGG 1 cut(s) 157
Hin1II CATG 8 cut(s) 34, 120, 226, 258, 272, 369, 479, 523
HinfI GANTC 4 cut(s) 14, 284, 395, 501
HpaII CCGG 1 cut(s) 157
HphI GGTGA 2 cut(s) 394, 539
Hpy188I TCNGA 1 cut(s) 13
Hpy188III TCNNGA 2 cut(s) 31, 339
HpyAV CCTTC 1 cut(s) 303
HpyCH4III ACNGT 1 cut(s) 325
HpyCH4V TGCA 2 cut(s) 41, 519
HpyF3I CTNAG 1 cut(s) 318
Hsp92II CATG 8 cut(s) 34, 120, 226, 258, 272, 369, 479, 523
Ksp22I TGATCA 1 cut(s) 292
Kzo9I GATC 3 cut(s) 49, 250, 292
LmnI GCTCC 3 cut(s) 159, 268, 352
LpnPI CCDG 5 cut(s) 170, 220, 247, 441, 496
Lsp1109I GCAGC 1 cut(s) 25
MabI ACCWGGT 1 cut(s) 233
MaeIII GTNAC 3 cut(s) 325, 380, 464
MalI GATC 3 cut(s) 51, 252, 294
MbiI CCGCTC 1 cut(s) 9
MboI GATC 3 cut(s) 49, 250, 292
MboII GAAGA 4 cut(s) 53, 56, 325, 363
MfeI CAATTG 1 cut(s) 97
MflI RGATCY 1 cut(s) 250
MhlI GDGCHC 2 cut(s) 164, 265
MluCI AATT 4 cut(s) 97, 408, 431, 531
MlyI GAGTC 2 cut(s) 8, 510
MmeI TCCRAC 1 cut(s) 176
MnlI CCTC 6 cut(s) 38, 64, 192, 253, 406, 517
MseI TTAA 1 cut(s) 21
MslI CAYNNNNRTG 1 cut(s) 138
MspI CCGG 1 cut(s) 157
MspR9I CCNGG 2 cut(s) 157, 235
MunI CAATTG 1 cut(s) 97
MvaI CCWGG 1 cut(s) 235
MvnI CGCG 3 cut(s) 193, 206, 352
NciI CCSGG 1 cut(s) 157
NdeI CATATG 1 cut(s) 77
NdeII GATC 3 cut(s) 49, 250, 292
NlaIII CATG 8 cut(s) 34, 120, 226, 258, 272, 369, 479, 523
NlaIV GGNNCC 1 cut(s) 252
NmuCI GTSAC 1 cut(s) 464
NspI RCATGY 1 cut(s) 226
PaeR7I CTCGAG 1 cut(s) 498
PagI TCATGA 1 cut(s) 30
PciI ACATGT 1 cut(s) 222
PfeI GAWTC 2 cut(s) 284, 395
PkrI GCNGC 1 cut(s) 40
PleI GAGTC 2 cut(s) 8, 509
PpsI GAGTC 2 cut(s) 8, 509
PscI ACATGT 1 cut(s) 222
Psp124BI GAGCTC 1 cut(s) 265
Psp6I CCWGG 1 cut(s) 233
PspEI GGTNACC 1 cut(s) 380
PspGI CCWGG 1 cut(s) 233
PspN4I GGNNCC 1 cut(s) 252
PspXI VCTCGAGB 1 cut(s) 498
PsuI RGATCY 1 cut(s) 250
RsaI GTAC 1 cut(s) 474
RsaNI GTAC 1 cut(s) 473
RseI CAYNNNNRTG 1 cut(s) 138
SacI GAGCTC 1 cut(s) 265
SaqAI TTAA 1 cut(s) 21
SatI GCNGC 1 cut(s) 39
Sau3AI GATC 3 cut(s) 49, 250, 292
SchI GAGTC 2 cut(s) 8, 510
ScrFI CCNGG 2 cut(s) 157, 235
SduI GDGCHC 2 cut(s) 164, 265
SetI ASST 8 cut(s) 30, 40, 239, 265, 349, 417, 484, 515
SexAI ACCWGGT 1 cut(s) 233
Sfr274I CTCGAG 1 cut(s) 498
SlaI CTCGAG 1 cut(s) 498
SmiMI CAYNNNNRTG 1 cut(s) 138
SmlI CTYRAG 1 cut(s) 498
SmoI CTYRAG 1 cut(s) 498
Sse9I AATT 4 cut(s) 97, 408, 431, 531
SsiI CCGC 3 cut(s) 7, 350, 392
SstI GAGCTC 1 cut(s) 265
StyD4I CCNGG 2 cut(s) 155, 233
StyI CCWWGG 1 cut(s) 246
TaaI ACNGT 1 cut(s) 325
TaqI TCGA 3 cut(s) 259, 303, 499
TasI AATT 4 cut(s) 97, 408, 431, 531
TatI WGTACW 1 cut(s) 472
TfiI GAWTC 2 cut(s) 284, 395
Tru1I TTAA 1 cut(s) 21
Tru9I TTAA 1 cut(s) 21
TseFI GTSAC 1 cut(s) 464
TseI GCWGC 1 cut(s) 38
Tsp45I GTSAC 1 cut(s) 464
TspDTI ATGAA 5 cut(s) 17, 47, 156, 461, 492
TspGWI ACGGA 2 cut(s) 184, 425
XapI RAATTY 1 cut(s) 431
XceI RCATGY 1 cut(s) 226
XhoI CTCGAG 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.