Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0003560

Physical Location & Seq

Genomic Coordinates Reverse (-)

66045100 .. 66046469

Gene Structure 1370 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00046690.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 780 bp

ATGGGTCGCGTCATCAGAGCTCAACGTAAGGGAGCGGGCTCCGTCTTCAAGTCCCACACCCACCACCGCAAGGGCCCGGCCCGCTTCCGCTCCCTCGACTACGGCGAGCGCAACGGCTACCTCAAGGGCGTCGTCACCGACATCATCCACGACCCCGGTCGCGGTGCCCCGCTCGCTCGCGTCAGCTTCCGTCATCCCTTCCGGTACAAGAAGCAGAACGAGCTCTTCGTCGCCGCCGAGGGGCTTTACACCGGCCAGTTTATTTACTGCGGGAAGAAGGCCAATTTGGTCGTCGGAAATGTGTTGCCGGTCAGATCTATTCCCGAGGGAGCTGTGGTGTGTAATGTCGAGCACCACGTCGGCGACCGCGGAACCCTAGCTAGGGCTTCGGGGGATTACGCTGTGATTATCAGTCACAACCCTGATAATGATACCTCAAGAATCAAGCTTCCTTCTGGTGCCAAGAAGATTGTTCCAAGTGGTTGCCGTGCCATGATTGGTCAAGTTGCAGGTGGAGGCAGAACTGAGAAGCCTATGCTCAAGGCCGGTAATGCTTACCACAAGTTCAGAGTGAAGAGGAACTGCTGGCCTAAGGTGCGTGGTGTGGCTATGAATCCTGTTGAGCATCCTCACGGAGGAGGTAACCACCAGCATATTGGCCATGCTAGCACAGTCAGGCGTGATGCTCCTCCTGGGCAGAAGGTTGGTCTCATTGCTGCCAGGAGAACCGGTCGTCTCAGAGGACAAGCTGCTGCAACTGCCTCCAAGGAGAAGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002181 GO:0003674 GO:0003676 GO:0003723 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0015934 GO:0019538 GO:0022625 GO:0022626 GO:0032991 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0071704 GO:0097159 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03010 map03010

Pfam Domains

Protein Families

Ribosomal_L2 Ribosomal_L2_C

Protein Analysis

259

Amino Acids

28.04

Weight (kDa)

10.81

Isoelectric Point (pI)

24.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ribosomal_L2_N	PF00181	2 - 90	3.3e-22	Ribosomal Proteins L2, RNA binding N-terminal domain
Ribosomal_L2_C	PF03947	98 - 224	1.3e-45	Ribosomal Proteins L2, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003560)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G18020 AT3G51190 AT4G36130
fragaria_vesca	FvH4_3g11370 FvH4_3g11370 FvH4_3g19850
malus_domestica	MD05G1264000.v1.1 MD10G1241900.v1.1
prunus_persica	Prupe.1G202200_v2.0.a1 Prupe.4G101600_v2.0.a1
pyrus_communis	pycom05g24810 pycom10g20260 pycom10g20270
rosa_chinensis	RchiOBHm_Chr5g0018321 RchiOBHm_Chr5g0033291
rosa_laevigata	RLG00000032351 RLG00000033468
rosa_multiflora	Rmu_co8428655.1_g000001 Rmu_sc0002206.1_g000007 Rmu_sc0005702.1_g000002 Rmu_sc0009715.1_g000002 Rmu_sc0011919.1_g000007
rosa_roxburghii	Rroxscaffold_1G00046690
rosa_rugosa	Rorug05G0041300 Rorug05G0139200
rosa_samantha	Rh5AG134500 Rh5AG230600 Rh5BG133300 Rh5BG231600 Rh5CG145000 Rh5CG260300 Rh5DG134700 Rh5DG236700
rosa_wichuraiana	Rw5G011760 Rw5G021080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	500
Acc36I	ACCTGC	1 cut(s)	500
AccB1I	GGYRCC	2 cut(s)	164, 458
AccBSI	CCGCTC	3 cut(s)	35, 90, 172
AccII	CGCG	4 cut(s)	9, 162, 180, 369
AcoI	YGGCCR	2 cut(s)	253, 658
AcyI	GRCGYC	1 cut(s)	129
AfaI	GTAC	1 cut(s)	206
AfiI	CCNNNNNNNGG	6 cut(s)	70, 161, 240, 381, 382, 635
AgeI	ACCGGT	1 cut(s)	728
AgsI	TTSAA	1 cut(s)	49
AjiI	CACGTC	1 cut(s)	358
AjnI	CCWGG	2 cut(s)	691, 719
AjuI	GAANNNNNNNTTGG	2 cut(s)	269, 301
AluBI	AGCT	7 cut(s)	20, 186, 223, 332, 380, 448, 749
AluI	AGCT	7 cut(s)	20, 186, 223, 332, 380, 448, 749
Alw21I	GWGCWC	3 cut(s)	22, 225, 354
Alw26I	GTCTC	2 cut(s)	713, 740
Ama87I	CYCGRG	1 cut(s)	323
AoxI	GGCC	7 cut(s)	73, 78, 253, 279, 543, 587, 658
ApaI	GGGCCC	1 cut(s)	77
ApeKI	GCWGC	3 cut(s)	716, 749, 752
AsiGI	ACCGGT	1 cut(s)	728
AspLEI	GCGC	1 cut(s)	111
AspS9I	GGNCC	3 cut(s)	73, 74, 79
AsuC2I	CCSGG	2 cut(s)	77, 156
AsuHPI	GGTGA	1 cut(s)	127
AsuNHI	GCTAGC	1 cut(s)	665
AvaI	CYCGRG	1 cut(s)	323
AxyI	CCTNAGG	1 cut(s)	591
BaeGI	GKGCMC	2 cut(s)	77, 169
BalI	TGGCCA	1 cut(s)	660
BanI	GGYRCC	2 cut(s)	164, 458
BanII	GRGCYC	4 cut(s)	22, 41, 77, 225
BbsI	GAAGAC	1 cut(s)	37
Bbv12I	GWGCWC	3 cut(s)	22, 225, 354
BbvI	GCAGC	3 cut(s)	703, 736, 739
BceAI	ACGGC	3 cut(s)	118, 130, 471
BciT130I	CCWGG	2 cut(s)	693, 721
BcnI	CCSGG	2 cut(s)	77, 156
BcoDI	GTCTC	2 cut(s)	713, 740
BfaI	CTAG	3 cut(s)	377, 381, 666
BfuAI	ACCTGC	1 cut(s)	500
BglII	AGATCT	1 cut(s)	314
BisI	GCNGC	4 cut(s)	234, 717, 750, 753
BlsI	GCNGC	4 cut(s)	235, 718, 751, 754
Bme1390I	CCNGG	4 cut(s)	77, 156, 693, 721
BmeT110I	CYCGRG	1 cut(s)	323
BmgBI	CACGTC	1 cut(s)	358
BmgT120I	GGNCC	3 cut(s)	73, 74, 79
BmiI	GGNNCC	5 cut(s)	40, 75, 166, 373, 460
BmrFI	CCNGG	4 cut(s)	77, 156, 693, 721
BmsI	GCATC	2 cut(s)	634, 673
BmtI	GCTAGC	1 cut(s)	669
BoxI	GACNNNNGTC	1 cut(s)	156
BpiI	GAAGAC	1 cut(s)	37
BpuEI	CTTGAG	3 cut(s)	107, 421, 524
BpuMI	CCSGG	2 cut(s)	77, 156
BsaHI	GRCGYC	1 cut(s)	129
BsaI	GGTCTC	1 cut(s)	713
BsaJI	CCNNGG	6 cut(s)	154, 237, 324, 367, 692, 765
BsaWI	WCCGGW	2 cut(s)	201, 728
BsaXI	ACNNNNNCTCC	4 cut(s)	321, 351, 715, 745
Bsc4I	CCNNNNNNNGG	6 cut(s)	70, 161, 240, 381, 382, 635
Bse118I	RCCGGY	4 cut(s)	251, 307, 545, 728
Bse1I	ACTGG	1 cut(s)	256
Bse21I	CCTNAGG	1 cut(s)	591
Bse3DI	GCAATG	1 cut(s)	711
BseBI	CCWGG	2 cut(s)	693, 721
BseDI	CCNNGG	6 cut(s)	154, 237, 324, 367, 692, 765
BseGI	GGATG	3 cut(s)	144, 193, 625
BseLI	CCNNNNNNNGG	6 cut(s)	70, 161, 240, 381, 382, 635
BseMI	GCAATG	1 cut(s)	711
BseMII	CTCAG	2 cut(s)	516, 751
BseNI	ACTGG	1 cut(s)	256
BseRI	GAGGAG	2 cut(s)	651, 678
BseSI	GKGCMC	2 cut(s)	77, 169
BseXI	GCAGC	3 cut(s)	703, 736, 739
Bsh1236I	CGCG	4 cut(s)	9, 162, 180, 369
Bsh1285I	CGRYCG	3 cut(s)	160, 367, 733
BshFI	GGCC	7 cut(s)	75, 80, 255, 281, 545, 589, 660
BshNI	GGYRCC	2 cut(s)	164, 458
BshTI	ACCGGT	1 cut(s)	728
BsiEI	CGRYCG	3 cut(s)	160, 367, 733
BsiHKAI	GWGCWC	3 cut(s)	22, 225, 354
BsiHKCI	CYCGRG	1 cut(s)	323
BsiSI	CCGG	7 cut(s)	77, 156, 202, 252, 308, 546, 729
BslFI	GGGAC	1 cut(s)	37
BslI	CCNNNNNNNGG	6 cut(s)	70, 161, 240, 381, 382, 635
BsmAI	GTCTC	2 cut(s)	713, 740
BsmBI	CGTCTC	1 cut(s)	740
BsmFI	GGGAC	1 cut(s)	37
BsnI	GGCC	7 cut(s)	75, 80, 255, 281, 545, 589, 660
Bso31I	GGTCTC	1 cut(s)	713
BsoBI	CYCGRG	1 cut(s)	323
Bsp120I	GGGCCC	1 cut(s)	73
Bsp1286I	GDGCHC	6 cut(s)	22, 41, 77, 169, 225, 354
Bsp143I	GATC	1 cut(s)	314
BspANI	GGCC	7 cut(s)	75, 80, 255, 281, 545, 589, 660
BspCNI	CTCAG	2 cut(s)	517, 750
BspFNI	CGCG	4 cut(s)	9, 162, 180, 369
BspLI	GGNNCC	5 cut(s)	40, 75, 166, 373, 460
BspMI	ACCTGC	1 cut(s)	500
BspOI	GCTAGC	1 cut(s)	669
BspQI	GCTCTTC	1 cut(s)	230
BspT107I	GGYRCC	2 cut(s)	164, 458
BspTNI	GGTCTC	1 cut(s)	713
BsrBI	CCGCTC	3 cut(s)	35, 90, 172
BsrDI	GCAATG	1 cut(s)	711
BsrFI	RCCGGY	4 cut(s)	251, 307, 545, 728
BsrI	ACTGG	1 cut(s)	256
BssAI	RCCGGY	4 cut(s)	251, 307, 545, 728
BssECI	CCNNGG	6 cut(s)	154, 237, 324, 367, 692, 765
BssMI	GATC	1 cut(s)	314
BssNI	GRCGYC	1 cut(s)	129
BssT1I	CCWWGG	1 cut(s)	765
Bst2UI	CCWGG	2 cut(s)	693, 721
Bst4CI	ACNGT	1 cut(s)	673
Bst6I	CTCTTC	2 cut(s)	230, 569
BstACI	GRCGYC	1 cut(s)	129
BstC8I	GCNNGC	7 cut(s)	37, 82, 107, 174, 178, 587, 667
BstDEI	CTNAG	3 cut(s)	525, 591, 737
BstDSI	CCRYGG	1 cut(s)	367
BstEII	GGTNACC	1 cut(s)	641
BstENI	CCTNNNNNAGG	1 cut(s)	633
BstF5I	GGATG	3 cut(s)	144, 193, 625
BstFNI	CGCG	4 cut(s)	9, 162, 180, 369
BstHHI	GCGC	1 cut(s)	111
BstKTI	GATC	1 cut(s)	317
BstMAI	GTCTC	2 cut(s)	713, 740
BstMBI	GATC	1 cut(s)	314
BstMCI	CGRYCG	3 cut(s)	160, 367, 733
BstMWI	GCNNNNNNNGC	7 cut(s)	81, 173, 220, 551, 595, 666, 758
BstNI	CCWGG	2 cut(s)	693, 721
BstPAI	GACNNNNGTC	1 cut(s)	156
BstPI	GGTNACC	1 cut(s)	641
BstSCI	CCNGG	4 cut(s)	75, 154, 691, 719
BstSLI	GKGCMC	2 cut(s)	77, 169
BstUI	CGCG	4 cut(s)	9, 162, 180, 369
BstV1I	GCAGC	3 cut(s)	703, 736, 739
BstV2I	GAAGAC	1 cut(s)	37
BstX2I	RGATCY	1 cut(s)	314
BstXI	CCANNNNNNTGG	1 cut(s)	656
BstYI	RGATCY	1 cut(s)	314
Bsu36I	CCTNAGG	1 cut(s)	591
BsuRI	GGCC	7 cut(s)	75, 80, 255, 281, 545, 589, 660
BtgI	CCRYGG	1 cut(s)	367
BtrI	CACGTC	1 cut(s)	358
BtsCI	GGATG	3 cut(s)	144, 193, 625
BveI	ACCTGC	1 cut(s)	500
Cac8I	GCNNGC	7 cut(s)	37, 82, 107, 174, 178, 587, 667
CfoI	GCGC	1 cut(s)	111
Cfr10I	RCCGGY	4 cut(s)	251, 307, 545, 728
Cfr13I	GGNCC	3 cut(s)	73, 74, 79
Cfr42I	CCGCGG	1 cut(s)	370
CseI	GACGC	2 cut(s)	118, 169
Csp6I	GTAC	1 cut(s)	205
CspAI	ACCGGT	1 cut(s)	728
CviAII	CATG	2 cut(s)	493, 662
CviQI	GTAC	1 cut(s)	205
DdeI	CTNAG	3 cut(s)	525, 591, 737
DpnI	GATC	1 cut(s)	316
DpnII	GATC	1 cut(s)	314
EaeI	YGGCCR	2 cut(s)	253, 658
Eam1104I	CTCTTC	2 cut(s)	230, 569
EarI	CTCTTC	2 cut(s)	230, 569
Ecl136II	GAGCTC	2 cut(s)	20, 223
Eco130I	CCWWGG	1 cut(s)	765
Eco24I	GRGCYC	4 cut(s)	22, 41, 77, 225
Eco31I	GGTCTC	1 cut(s)	713
Eco53kI	GAGCTC	2 cut(s)	20, 223
Eco81I	CCTNAGG	1 cut(s)	591
Eco88I	CYCGRG	1 cut(s)	323
Eco91I	GGTNACC	1 cut(s)	641
EcoICRI	GAGCTC	2 cut(s)	20, 223
EcoNI	CCTNNNNNAGG	1 cut(s)	633
EcoO109I	RGGNCCY	1 cut(s)	73
EcoO65I	GGTNACC	1 cut(s)	641
EcoRII	CCWGG	2 cut(s)	691, 719
EcoT14I	CCWWGG	1 cut(s)	765
EcoT38I	GRGCYC	4 cut(s)	22, 41, 77, 225
ErhI	CCWWGG	1 cut(s)	765
Esp3I	CGTCTC	1 cut(s)	740
FaeI	CATG	2 cut(s)	496, 665
FaiI	YATR	5 cut(s)	494, 536, 611, 654, 663
FaqI	GGGAC	1 cut(s)	37
FatI	CATG	2 cut(s)	492, 661
FauI	CCCGC	4 cut(s)	28, 89, 177, 263
Fnu4HI	GCNGC	4 cut(s)	234, 717, 750, 753
FokI	GGATG	3 cut(s)	131, 180, 612
FriOI	GRGCYC	4 cut(s)	22, 41, 77, 225
Fsp4HI	GCNGC	4 cut(s)	234, 717, 750, 753
FspBI	CTAG	3 cut(s)	377, 381, 666
GlaI	GCGC	1 cut(s)	110
GluI	GCNGC	4 cut(s)	234, 717, 750, 753
HaeIII	GGCC	7 cut(s)	75, 80, 255, 281, 545, 589, 660
HapII	CCGG	7 cut(s)	77, 156, 202, 252, 308, 546, 729
HgaI	GACGC	2 cut(s)	118, 169
HhaI	GCGC	1 cut(s)	111
Hin1I	GRCGYC	1 cut(s)	129
Hin1II	CATG	2 cut(s)	496, 665
Hin6I	GCGC	1 cut(s)	109
HinP1I	GCGC	1 cut(s)	109
HindIII	AAGCTT	1 cut(s)	446
HinfI	GANTC	2 cut(s)	441, 613
HpaII	CCGG	7 cut(s)	77, 156, 202, 252, 308, 546, 729
HphI	GGTGA	1 cut(s)	127
Hpy188I	TCNGA	5 cut(s)	17, 296, 314, 569, 740
Hpy188III	TCNNGA	2 cut(s)	323, 438
Hpy99I	CGWCG	4 cut(s)	134, 233, 296, 362
HpyAV	CCTTC	5 cut(s)	208, 271, 462, 694, 766
HpyCH4III	ACNGT	1 cut(s)	673
HpyCH4IV	ACGT	2 cut(s)	25, 357
HpyCH4V	TGCA	2 cut(s)	509, 755
HpyF10VI	GCNNNNNNNGC	7 cut(s)	81, 173, 220, 551, 595, 666, 758
HpyF3I	CTNAG	3 cut(s)	525, 591, 737
HpySE526I	ACGT	2 cut(s)	25, 357
Hsp92I	GRCGYC	1 cut(s)	129
Hsp92II	CATG	2 cut(s)	496, 665
HspAI	GCGC	1 cut(s)	109
KspI	CCGCGG	1 cut(s)	370
Kzo9I	GATC	1 cut(s)	314
LguI	GCTCTTC	1 cut(s)	230
LmnI	GCTCC	5 cut(s)	32, 44, 95, 329, 691
Lsp1109I	GCAGC	3 cut(s)	703, 736, 739
LweI	GCATC	2 cut(s)	634, 673
MaeI	CTAG	3 cut(s)	377, 381, 666
MaeII	ACGT	2 cut(s)	25, 357
MaeIII	GTNAC	3 cut(s)	133, 413, 641
MalI	GATC	1 cut(s)	316
MbiI	CCGCTC	3 cut(s)	35, 90, 172
MboI	GATC	1 cut(s)	314
MboII	GAAGA	5 cut(s)	37, 217, 286, 478, 586
MflI	RGATCY	1 cut(s)	314
MhlI	GDGCHC	6 cut(s)	22, 41, 77, 169, 225, 354
MlsI	TGGCCA	1 cut(s)	660
MluCI	AATT	1 cut(s)	283
MluNI	TGGCCA	1 cut(s)	660
MmeI	TCCRAC	1 cut(s)	274
Mox20I	TGGCCA	1 cut(s)	660
MscI	TGGCCA	1 cut(s)	660
Msp20I	TGGCCA	1 cut(s)	660
MspA1I	CMGCKG	1 cut(s)	369
MspI	CCGG	7 cut(s)	77, 156, 202, 252, 308, 546, 729
MspR9I	CCNGG	4 cut(s)	77, 156, 693, 721
MvaI	CCWGG	2 cut(s)	693, 721
MvnI	CGCG	4 cut(s)	9, 162, 180, 369
MwoI	GCNNNNNNNGC	7 cut(s)	81, 173, 220, 551, 595, 666, 758
NciI	CCSGG	2 cut(s)	77, 156
NdeII	GATC	1 cut(s)	314
NheI	GCTAGC	1 cut(s)	665
NlaIII	CATG	2 cut(s)	496, 665
NlaIV	GGNNCC	5 cut(s)	40, 75, 166, 373, 460
NmeAIII	GCCGAG	1 cut(s)	262
NmuCI	GTSAC	2 cut(s)	133, 413
PaqCI	CACCTGC	1 cut(s)	500
PciSI	GCTCTTC	1 cut(s)	230
PcsI	WCGNNNNNNNCGW	4 cut(s)	102, 225, 234, 354
PfeI	GAWTC	2 cut(s)	441, 613
PinAI	ACCGGT	1 cut(s)	728
PkrI	GCNGC	4 cut(s)	235, 718, 751, 754
PshAI	GACNNNNGTC	1 cut(s)	156
Psp124BI	GAGCTC	2 cut(s)	22, 225
Psp6I	CCWGG	2 cut(s)	691, 719
PspEI	GGTNACC	1 cut(s)	641
PspGI	CCWGG	2 cut(s)	691, 719
PspN4I	GGNNCC	5 cut(s)	40, 75, 166, 373, 460
PspOMI	GGGCCC	1 cut(s)	73
PspPI	GGNCC	3 cut(s)	73, 74, 79
PsuI	RGATCY	1 cut(s)	314
RsaI	GTAC	1 cut(s)	206
RsaNI	GTAC	1 cut(s)	205
SacI	GAGCTC	2 cut(s)	22, 225
SacII	CCGCGG	1 cut(s)	370
SapI	GCTCTTC	1 cut(s)	230
SatI	GCNGC	4 cut(s)	234, 717, 750, 753
Sau3AI	GATC	1 cut(s)	314
Sau96I	GGNCC	3 cut(s)	73, 74, 79
ScrFI	CCNGG	4 cut(s)	77, 156, 693, 721
SduI	GDGCHC	6 cut(s)	22, 41, 77, 169, 225, 354
SfaNI	GCATC	2 cut(s)	634, 673
Sfr303I	CCGCGG	1 cut(s)	370
SgrBI	CCGCGG	1 cut(s)	370
SmlI	CTYRAG	3 cut(s)	122, 436, 539
SmoI	CTYRAG	3 cut(s)	122, 436, 539
Sse9I	AATT	1 cut(s)	283
SspMI	CTAG	3 cut(s)	377, 381, 666
SstI	GAGCTC	2 cut(s)	22, 225
StyD4I	CCNGG	4 cut(s)	75, 154, 691, 719
StyI	CCWWGG	1 cut(s)	765
TaaI	ACNGT	1 cut(s)	673
TaiI	ACGT	2 cut(s)	28, 360
TaqI	TCGA	2 cut(s)	96, 348
TasI	AATT	1 cut(s)	283
TauI	GCSGC	1 cut(s)	236
TfiI	GAWTC	2 cut(s)	441, 613
TseFI	GTSAC	2 cut(s)	133, 413
TseI	GCWGC	3 cut(s)	716, 749, 752
Tsp45I	GTSAC	2 cut(s)	133, 413
TspDTI	ATGAA	1 cut(s)	626
TspGWI	ACGGA	3 cut(s)	31, 179, 648
XagI	CCTNNNNNAGG	1 cut(s)	633
XcmI	CCANNNNNNNNNTGG	1 cut(s)	653
XspI	CTAG	3 cut(s)	377, 381, 666

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.