Rroxscaffold_1G00068590

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0012693
Physical Location & Seq
Forward (+)
89754794 .. 89755237
444 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00068590.1

Sequence Viewer

Length: 444 bp
ATGCTCAACCGTGGCTTCTCCGACTCCGCCTCTATTAACTGCTTGCTCGGCCTAGACTACTGCTGCGGCGCCGCCGTGAACAATTCAGATTCGAGAGGAGGAGAAGCAGAGGCTTCTGCGGCGGCGAATTGCAGGATCAGAAGTCGTTTAGGTCAACATCGTCGTCTCCGTCGCTGGCGAATTTGCCTCCGATTCGGGTCCACGTGGAAGACAACAGTGGGAGTAGTCGCTGTGGGAATAGAGCGCAGGATCAGAAGGTTGGGATTGGAGCAGTTTGCTCAGATGACGAACGATCCGATCACAGTGGATATCGGAAACTGCTTCCTCCTCAGCCTCAGCAATAGAGTCTACCTCCTCAATCGCAGCCCAAATCTACTGGAATTTTTTATTTTCCGTCGCCAGATTCAGTGTCAATGTATGCTTTGCTTGTCACCTACAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

147

Amino Acids

16.58

Weight (kDa)

9.79

Isoelectric Point (pI)

54.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 68
AccI GTMKAC 1 cut(s) 348
AciI CCGC 5 cut(s) 27, 66, 72, 119, 122
AclWI GGATC 3 cut(s) 143, 257, 287
AcsI RAATTY 2 cut(s) 180, 380
AcvI CACGTG 1 cut(s) 204
AcyI GRCGYC 1 cut(s) 69
Alw26I GTCTC 1 cut(s) 170
AlwI GGATC 3 cut(s) 143, 257, 287
AoxI GGCC 1 cut(s) 49
ApeKI GCWGC 2 cut(s) 63, 363
ApoI RAATTY 2 cut(s) 180, 380
AspLEI GCGC 2 cut(s) 71, 246
AspS9I GGNCC 1 cut(s) 198
AsuHPI GGTGA 1 cut(s) 423
AvaII GGWCC 1 cut(s) 198
BanI GGYRCC 1 cut(s) 68
BbrPI CACGTG 1 cut(s) 204
BbsI GAAGAC 1 cut(s) 215
BbvCI CCTCAGC 2 cut(s) 329, 335
BbvI GCAGC 2 cut(s) 50, 375
BceAI ACGGC 1 cut(s) 59
BcoDI GTCTC 1 cut(s) 170
BfaI CTAG 1 cut(s) 53
BfoI RGCGCY 1 cut(s) 72
BisI GCNGC 6 cut(s) 64, 67, 72, 120, 123, 364
BlsI GCNGC 6 cut(s) 65, 68, 73, 121, 124, 365
Bme18I GGWCC 1 cut(s) 198
BmgT120I GGNCC 1 cut(s) 198
BmiI GGNNCC 2 cut(s) 70, 199
BpiI GAAGAC 1 cut(s) 215
BplI GAGNNNNNCTC 2 cut(s) 336, 368
Bpu10I CCTNAGC 2 cut(s) 329, 335
BsaAI YACGTR 1 cut(s) 204
BsaHI GRCGYC 1 cut(s) 69
BsaJI CCNNGG 1 cut(s) 10
Bse1I ACTGG 1 cut(s) 381
BseDI CCNNGG 1 cut(s) 10
BseMII CTCAG 3 cut(s) 293, 343, 349
BseNI ACTGG 1 cut(s) 381
BseRI GAGGAG 4 cut(s) 111, 114, 317, 344
BseXI GCAGC 2 cut(s) 50, 375
BshFI GGCC 1 cut(s) 51
BshNI GGYRCC 1 cut(s) 68
BsmAI GTCTC 1 cut(s) 170
BsmBI CGTCTC 1 cut(s) 170
BsnI GGCC 1 cut(s) 51
Bsp143I GATC 4 cut(s) 135, 249, 292, 297
BspACI CCGC 5 cut(s) 27, 66, 72, 119, 122
BspANI GGCC 1 cut(s) 51
BspCNI CTCAG 3 cut(s) 292, 342, 348
BspLI GGNNCC 2 cut(s) 70, 199
BspPI GGATC 3 cut(s) 143, 257, 287
BspT107I GGYRCC 1 cut(s) 68
BsrI ACTGG 1 cut(s) 381
BssECI CCNNGG 1 cut(s) 10
BssMI GATC 4 cut(s) 135, 249, 292, 297
BssNI GRCGYC 1 cut(s) 69
Bst4CI ACNGT 3 cut(s) 11, 217, 304
BstACI GRCGYC 1 cut(s) 69
BstBAI YACGTR 1 cut(s) 204
BstC8I GCNNGC 2 cut(s) 44, 176
BstDEI CTNAG 3 cut(s) 279, 329, 335
BstDSI CCRYGG 1 cut(s) 10
BstH2I RGCGCY 1 cut(s) 72
BstHHI GCGC 2 cut(s) 71, 246
BstKTI GATC 4 cut(s) 138, 252, 295, 300
BstMAI GTCTC 1 cut(s) 170
BstMBI GATC 4 cut(s) 135, 249, 292, 297
BstMWI GCNNNNNNNGC 2 cut(s) 48, 119
BstV1I GCAGC 2 cut(s) 50, 375
BstV2I GAAGAC 1 cut(s) 215
BsuRI GGCC 1 cut(s) 51
BtgI CCRYGG 1 cut(s) 10
BtsIMutI CAGTG 3 cut(s) 222, 309, 413
Cac8I GCNNGC 2 cut(s) 44, 176
CfoI GCGC 2 cut(s) 71, 246
Cfr13I GGNCC 1 cut(s) 198
CviJI RGCY 5 cut(s) 15, 51, 113, 333, 366
CviKI_1 RGCY 5 cut(s) 15, 51, 113, 333, 366
DdeI CTNAG 3 cut(s) 279, 329, 335
DinI GGCGCC 1 cut(s) 70
DpnI GATC 4 cut(s) 137, 251, 294, 299
DpnII GATC 4 cut(s) 135, 249, 292, 297
EciI GGCGGA 1 cut(s) 16
Eco32I GATATC 1 cut(s) 310
Eco47I GGWCC 1 cut(s) 198
Eco72I CACGTG 1 cut(s) 204
EcoRV GATATC 1 cut(s) 310
EgeI GGCGCC 1 cut(s) 70
EheI GGCGCC 1 cut(s) 70
Esp3I CGTCTC 1 cut(s) 170
FaiI YATR 1 cut(s) 419
FblI GTMKAC 1 cut(s) 348
Fnu4HI GCNGC 6 cut(s) 64, 67, 72, 120, 123, 364
Fsp4HI GCNGC 6 cut(s) 64, 67, 72, 120, 123, 364
FspBI CTAG 1 cut(s) 53
GlaI GCGC 2 cut(s) 70, 245
GluI GCNGC 6 cut(s) 64, 67, 72, 120, 123, 364
HaeII RGCGCY 1 cut(s) 72
HaeIII GGCC 1 cut(s) 51
HhaI GCGC 2 cut(s) 71, 246
Hin1I GRCGYC 1 cut(s) 69
Hin6I GCGC 2 cut(s) 69, 244
HinP1I GCGC 2 cut(s) 69, 244
HincII GTYRAC 1 cut(s) 155
HindII GTYRAC 1 cut(s) 155
HinfI GANTC 5 cut(s) 23, 89, 192, 345, 403
HphI GGTGA 1 cut(s) 423
Hpy166II GTNNAC 4 cut(s) 79, 155, 201, 349
Hpy188I TCNGA 8 cut(s) 22, 88, 140, 191, 254, 282, 297, 314
Hpy188III TCNNGA 1 cut(s) 93
Hpy8I GTNNAC 4 cut(s) 79, 155, 201, 349
Hpy99I CGWCG 3 cut(s) 165, 174, 399
HpyAV CCTTC 1 cut(s) 249
HpyCH4III ACNGT 3 cut(s) 11, 217, 304
HpyCH4IV ACGT 1 cut(s) 203
HpyCH4V TGCA 1 cut(s) 132
HpyF10VI GCNNNNNNNGC 2 cut(s) 48, 119
HpyF3I CTNAG 3 cut(s) 279, 329, 335
HpySE526I ACGT 1 cut(s) 203
Hsp92I GRCGYC 1 cut(s) 69
HspAI GCGC 2 cut(s) 69, 244
KasI GGCGCC 1 cut(s) 68
Kzo9I GATC 4 cut(s) 135, 249, 292, 297
LmnI GCTCC 1 cut(s) 268
LpnPI CCDG 5 cut(s) 118, 160, 232, 362, 413
Lsp1109I GCAGC 2 cut(s) 50, 375
MaeI CTAG 1 cut(s) 53
MaeII ACGT 1 cut(s) 203
MaeIII GTNAC 1 cut(s) 429
MalI GATC 4 cut(s) 137, 251, 294, 299
MboI GATC 4 cut(s) 135, 249, 292, 297
MboII GAAGA 1 cut(s) 220
MluCI AATT 4 cut(s) 82, 127, 180, 380
Mly113I GGCGCC 1 cut(s) 69
MlyI GAGTC 2 cut(s) 17, 354
MmeI TCCRAC 1 cut(s) 45
MseI TTAA 1 cut(s) 36
MwoI GCNNNNNNNGC 2 cut(s) 48, 119
NarI GGCGCC 1 cut(s) 69
NdeII GATC 4 cut(s) 135, 249, 292, 297
NlaIV GGNNCC 2 cut(s) 70, 199
NmeAIII GCCGAG 1 cut(s) 27
NmuCI GTSAC 1 cut(s) 429
PcsI WCGNNNNNNNCGW 2 cut(s) 166, 293
PfeI GAWTC 3 cut(s) 89, 192, 403
PkrI GCNGC 6 cut(s) 65, 68, 73, 121, 124, 365
PleI GAGTC 2 cut(s) 17, 353
PluTI GGCGCC 1 cut(s) 72
PmaCI CACGTG 1 cut(s) 204
PmlI CACGTG 1 cut(s) 204
PpsI GAGTC 2 cut(s) 17, 353
Ppu21I YACGTR 1 cut(s) 204
PspCI CACGTG 1 cut(s) 204
PspN4I GGNNCC 2 cut(s) 70, 199
PspPI GGNCC 1 cut(s) 198
SaqAI TTAA 1 cut(s) 36
SatI GCNGC 6 cut(s) 64, 67, 72, 120, 123, 364
Sau3AI GATC 4 cut(s) 135, 249, 292, 297
Sau96I GGNCC 1 cut(s) 198
SchI GAGTC 2 cut(s) 17, 354
SetI ASST 5 cut(s) 154, 206, 260, 354, 436
SfoI GGCGCC 1 cut(s) 70
SinI GGWCC 1 cut(s) 198
Sse9I AATT 4 cut(s) 82, 127, 180, 380
SsiI CCGC 5 cut(s) 27, 66, 72, 119, 122
SspDI GGCGCC 1 cut(s) 68
SspMI CTAG 1 cut(s) 53
TaaI ACNGT 3 cut(s) 11, 217, 304
TaiI ACGT 1 cut(s) 206
TaqI TCGA 1 cut(s) 92
TasI AATT 4 cut(s) 82, 127, 180, 380
TauI GCSGC 4 cut(s) 69, 74, 122, 125
TfiI GAWTC 3 cut(s) 89, 192, 403
Tru1I TTAA 1 cut(s) 36
Tru9I TTAA 1 cut(s) 36
TscAI CASTG 3 cut(s) 222, 309, 413
TseFI GTSAC 1 cut(s) 429
TseI GCWGC 2 cut(s) 63, 363
Tsp45I GTSAC 1 cut(s) 429
TspGWI ACGGA 2 cut(s) 158, 383
TspRI CASTG 3 cut(s) 222, 309, 413
VpaK11BI GGWCC 1 cut(s) 198
XapI RAATTY 2 cut(s) 180, 380
XmiI GTMKAC 1 cut(s) 348
XspI CTAG 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.